Maintenance of hematopoiesis depends upon rare hematopoietic stem cells (HSCs), which can persist over an organism’s lifetime. It is conceivable that they must maintain a high degree of genetic stability; otherwise recurring exposure to genotoxins and accumulation of genetic changes could result in genomic instability and malignancy or cell death. We have focused on the response of HSCs and primitive hematopoietic cells to highly toxic DNA double-strand breaks (DSBs). Using assays to detect break rejoining and kinetics of early DSB response foci, we determined that non-cycling human HSC-containing cells display delayed break rejoining kinetics and persistent γH2AX and 53BP1 foci compared to cycling counterparts, more differentiated hematopoietic cells and human primary fibroblasts. In contrast, when stimulated to cycle, these HSC-containing cells are quite efficient at repairing breaks and resolving foci. These data suggest that the DNA damage response may be unusually prolonged in non-cycling primitive hematopoietic cells.

Maintenance of hematopoiesis depends upon rare hematopoietic stem cells (HSCs), which can persist over an organism’s lifetime. It is conceivable that they must maintain a high degree of genetic stability; otherwise recurring exposure to genotoxins and accumulation of genetic changes could result in genomic instability and malignancy or cell death. We have focused on the response of HSCs and primitive hematopoietic cells to highly toxic DNA double-strand breaks (DSBs). Using assays to detect break rejoining and kinetics of early DSB response foci, we determined that non-cycling human HSC-containing cells display delayed break rejoining kinetics and persistent γH2AX and 53BP1 foci compared to cycling counterparts, more differentiated hematopoietic cells and human primary fibroblasts. In contrast, when stimulated to cycle, these HSC-containing cells are quite efficient at repairing breaks and resolving foci. These data suggest that the DNA damage response may be unusually prolonged in non-cycling primitive hematopoietic cells.

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Cloud computing has been developed rapidly in recent years, and offers novel concepts and innovations in computer use. One application of cloud computing is that people can designate a proxy to help them to execute a number of tasks in certain situations instead of undertaking all tasks themselves. With this application, people can benefit from the proxy; however, some information is revealed to the proxy, such as their activities, and private data. That is, the proxy is aware of the actions of people through delegation processes, and proxy re-encryption which is a cryptographic primitive has been proposed to solve this problem. In the proxy re-encryption system, when a user (e.g., Alice) wants to send a ciphertext that is encrypted by her secret key and stored in the cloud to another user (e.g., Bob), she can designate a proxy to transform the ciphertext into a different ciphertext that can be decrypted by Bob¡¦s private key. Based on attribute-based encryption and proxy re-encryption, we propose attribute-based proxy re-encryption with bilinear pairing. Furthermore, in the proposed scheme, third paries cannot decrypt the ciphertext if they do no have matching attributes, regardless of being helped by proxy. Finally, we offer security proofs to demonstrate that the proposed scheme satisfies the essential requirements of attribute-based encryption schemes and proxy re-encryption schemes.

Diss.--Freiburg im Breisgau. / Bibliogr. p. 344-357. Index.

Dissertation--München--Ludwig-Maximilians-Universität, 1998-1999. Titre de soutenance : Die Tugend der koptischen Mönche. Christliches Gedankengut oder kulturelles Erbe Altägyptens ? / Contient des textes coptes avec leur traduction allemande, et des textes égyptiens traduits en allemand. Commentaire en allemand. Bibliogr. p. 290-298.

Texte remanié de: Thèse de doctorat--Philosophie--Lille 3, 2003. / Bibliogr. p. 257-270. L'ISSN exact est : 1292-430X.

Thèse d'exercice : Médecine. Médecine générale : Paris 12 : 2006. / Titre provenant de l'écran-titre. Bibliogr. f. 101-116.

Thèse de doctorat : Philosophie : Paris 12 : 2005. / Version électronique uniquement consultable au sein de l'Université Paris 12 (Intranet). Titre provenant de l'écran-titre. Bibliogr. : 612 réf.