Galaxy redshift surveys are powerful probes of cosmology. Yet, in order to fully exploit the information contained in galaxy surveys, we need to improve upon our understanding of the structure formation in the Universe. Galaxies are formed/observed at late times when the density field is no longer linear so that understanding non-linearities is essential. In this thesis, we show that, at high redshifts, we can accurately model the galaxy power spectrum in redshift space by using the standard cosmological perturbation theory. Going beyond the power spectrum, we can use the three-point function, or the bispectrum, to gain important information on the early universe as well as on the galaxy formation via measurements of primordial non-Gaussianity and galaxy bias. We show that the galaxy bispectrum is more sensitive to primordial non-Gaussianities than previously recognized, making high-redshift galaxy surveys a particularly potent probe of the physics of inflation. Weak lensing offers yet another way of probing cosmology. By cross correlating the angular position of galaxies with the shear measurement from galaxy lensing or CMB lensing, we also show that one can obtain the information on cosmological distance scale, the galaxy bias, and the primordial non Gaussianity from weak lensing method. / text

Submitted by PPG Letras (letraspg@pucrs.br) on 2018-02-06T11:39:42Z No. of bitstreams: 1 Tese_NadjaVoss.pdf: 3725438 bytes, checksum: bfafd5360bdd935c77abbcbb31e2889a (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-02-07T13:19:51Z (GMT) No. of bitstreams: 1 Tese_NadjaVoss.pdf: 3725438 bytes, checksum: bfafd5360bdd935c77abbcbb31e2889a (MD5) / Made available in DSpace on 2018-02-07T13:24:31Z (GMT). No. of bitstreams: 1 Tese_NadjaVoss.pdf: 3725438 bytes, checksum: bfafd5360bdd935c77abbcbb31e2889a (MD5) Previous issue date: 2018-01-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This essay, which is the second volume of the thesis, deals with the analysis of the documents of process of the novel Dentro, by my authorship. It is a hybrid novel between literature and visual arts, inscribed in Liberature, a Polish movement where the wholeness of the book is fundamental. No longer is the author's role only the literary text, but all of the questions relating to the object book. In Liberature, images, typography, cover, bookbinding, etc., have as much authorial meaning as the linguistic and, for such, they should be taken care of by the author. This essay also includes the mapping of the Primordial Image ? a concept that I have being theorizing since 2012 ?, a fractal and rhizomatic gear, that is denominator of creation, within this hybrid work. Thus, the process of creating a hybrid, liberatic work that leads to the extreme of Liberature?s concept, encompassing the whole process under a singe person is analyzed. As so how this Primordial Image drives the creation, mainly in a liberatic creation which, as such, does not end with the linguistic, but extendsand unfolds in each gesture. / Este ensaio, segundo volume da tese, traz a an?lise dos documentos de processo da novela Dentro, de autoria pr?pria. Uma novela h?brida entre literatura e artes visuais, inscrita na Libertura, movimento polon?s onde a integralidade do livro ? fundamental. N?o ? mais papel do autor apenas o texto liter?rio, mas sim todas as quest?es acerca do objeto livro. Na Liberatura, imagens, tipografia, capa, formas de encaderna??o, etc., possuem tanta significa??o autoral quanto o lingu?stico e, por tal, devem ficar a encargo do autor. Este ensaio traz ainda o mapeamento da Imagem Primordial ? conceito que venho teorizando desde 2012 ?, uma engrenagem fractal e rizom?tica, denominadora da cria??o, no seio dessa obra h?brida. Assim, ? analisado aqui o processo de cria??o de uma obra h?brida, liber?tica, que leva ao extremo o conceito de Liberatura, abarcando absolutamente todo o processo sob uma ?nica pessoa. E, como essa Imagem Primordial conduz a cria??o, principalmente uma cria??o liber?tica que, por tal, n?o finda com o lingu?stico, mas se estende e descortina em cada gesto.

Introdução: a alta prevalência do câncer e o aumento significativo da sobrevivência em longo prazo geraram interesse quanto à preservação da fertilidade em mulheres jovens expostas a quimioterapia e radioterapia. Neste sentido estudos de congelamento de tecido ovariano para posterior transplante, abriram uma nova perspectiva de aplicação no tratamento e prevenção da infertilidade feminina. Objetivos: comparar dois protocolos de congelamento de tecido ovariano, um lento não automatizado e um por vitrificação, com o intuito de avaliar a viabilidade dos tecidos para posterior transplante autólogo. Método: Foram utilizadas 30 camundongos fêmea CF1 com aproximadamente 8 semanas e pesando 29,29g±2,9. •Os ovários extraídos foram vitrificados ou congelados, mantidos em nitrogênio líquido por 30 dias e descongelados. Após o descongelamento, o ovário esquerdo foi destinado às análises histológicas e caracterização por imuno histoquímica para o marcador mouse vasa homologue (MVH) e o ovário direito foi utilizado para os testes de viabilidade celular com exclusão por azul de trypan. Resultados: Nas análises de Hematoxilina e Eosina (HE) foram contados folículos primordiais, primários, pré-antrais e antrais. Não houve diferença significativa na proporção de folículos primordiais, primários e pré-antrais após descongelamento entre os grupos testados. A contagem de folículos antrais foi significativamente maior no grupo de vitrificação (p = 0,004). No ensaio de imunohistoquímica para o marcador MVH, folículos MVH + e MVH- foram contados e comparados com o número total de folículos. O grupo congelamento lento apresentou maior número de células não marcadas (p = 0,012). Conclusão: Embora ambos os protocolos tenham apresentado resultados semelhantes na análise histológica das contagens foliculares, o protocolo de vitrificação foi significativamente melhor para preservar a população de células tronco ovarianas. / Introduction: The high prevalence of cancer and the significant increase in long-term survival have generated interest as the preservation of fertility in young women exposed to chemotherapy and radiotherapy. Experimental techniques have been tried in an attempt to reverse the ovarian failure induced by these treatments. In this regard studies of ovarian tissue freezing for subsequent transplantation disclose a new application perspective in the treatment and prevention of female infertility. Objective: two ovarian tissue freezing protocols were tested, a non-automated slow-freezing and by vitrification, in order to assess the viability of the tissues for subsequent autologous transplantation. Methods: as ovaries donors, were used 30 female CF1 mice approximately 8 weeks and weighing 29,29g±2,9. • The ovaries were vitrified or frozen, stored in liquid nitrogen for 30 days and thawed. After thawing, the left ovary was intended for histological and immunohistochemical characterization by histochemical marker for MVH and right ovary was used for the tests with cell viability by trypan blue exclusion. Results: In HE slides was counting primordial, primary, pre antral and antral follicles. No significant difference was found in the proportion of high-quality primordial, primary and pre antral follicles after thawing/warming in the slow-freezing and vitrification group, respectively. The antral follicle counting was significant higher in vitrification group (p=0,004). In immunohistochemistry assay for MVH Antibody , MVH+ and MVH- follicles were counted and compared with the total number of follicles and slow freeze group had a higher number of not marked cells (p=0,012). Conclusion: Although both protocols showed similar results in the histological analysis for follicular counts, the vitrification protocol was significantly better for preserve the ovarian stem cell population.

The inflationary paradigm is one which was designed to answer questions that arose from classical Hot Big Bang cosmology. The period of rapid expansion in the early Universe provides a mechanism to solve the flatness, horizon and relic problems. More importantly, since the theory was first introduced it has been realised that it also provides a mechanism to generate the initial perturbations from which structure in the Universe can grow. In the zoo of potential inflationary models there is a dominant class: slow-roll inflation. The idea that the energy density of the inflationary field is dominated by its potential highly simplifies the calculations required to predict observable quantities. This simplification relies on all the information required to know the subsequent dynamics of the field to be encoded in the space Φ-Φ̇; it must be an effective phase space. I show that Φ-Φ̇ can be considered to be such a space for the most general scalar-tensor theory which gives second-order equations of motion: Horndeski theory. There are theoretical issues associated with this reduction that are illuminated through specific examples in which they occur. A theoretical issue with inflation is that there is an overabundance of models, with some capable of predicting any value of the possible observables. The second block of work in this thesis looks at a particular set of models that make the same observational prediction. These 'attractor' models utilise a non-minimal coupling between the inflationary fields and gravity and are studied in depth, both in the case of one and several fields. Firstly, I examine the Universal Attractors, a single field subset of these models. I show, in detail, the observational prediction such a model makes in the case of a strong non-minimal coupling and then examine the constraints it would be possible to put on such a coupling if a confirmed detection of primordial gravitational waves was made. Despite the discussion existing in the literature there is a small deviation of the Universal Attractor models from the predictions of the Starobinsky model. Furthermore, the coupling, ξ is found to be constrained so that |ξ| < 1 in the case where there a level of detectable primordial tensor modes. While the attractor models have an effective one-field description in reality there are several other fields that are assumed to be fixed during the inflationary phase. This claim requires careful examination as the field-space of the models generally is not flat. This curvature can cause a destabilising effect with certain parameters and so I investigate how susceptible the α-attractors and related models are to the destabilisation. A key result of this chapter is to highlight how important it is to not rely on the slow-roll approximation when assessing the effect of the instability, as the region where the effect begins to become large corresponds with the region where slow-roll begins to break down. Assuming the slow-roll approximation is valid leads to an over-estimation of the effect that the instability mechanism has. Despite this, some of the models considered are seen to experience the instability for certain ranges of model parameters. Making the assumption that any occurrence of the instability will, at the very least, move the observational prediction of the model outside the currently constrained range allows a constraint on the model parameter in question which directly translates to a theoretical lower bound on the tensor-scalar ratio, r > 0.0005.

The studies in this thesis examined piglet gonocyte identification, isolation, purification, preservation and potential for initiation of spermatogenesis after transplantation into irradiated recipient testes. As a first step, we characterized a previously non-described auto-fluorescence in the piglet testis tissue. This auto-fluorescence mainly originated from granules among the testis interstitial cells, and we found that its interference with immuno-fluorescence can be overcome using Sudan black staining. We also showed that porcine gonocytes can be specifically labelled with the lectin Dolichos biflorus agglutinin (DBA). To optimize gonocyte isolation, we found that ~9-fold more live cells could be harvested by enzymatic digestion of testis tissues than with mechanical methods. However, the proportion of gonocytes (~7%) did not differ between the mechanical and enzymatic methods of testis cell isolation. We then developed a novel three-step strategy for isolation of gonocytes by combining enzymatic digestion and vortexing, resulting in a gonocyte proportion of ~40% (~5-fold more than that from conventional methods). For short-term preservation of testis cells, we found that the survival of testis cells under hypothermic conditions was dependent on the cell type, and affected by storage duration, temperature and medium used. More than 80% of live testis cells survived the 6-day hypothermic preservation period in 20% FBS-L15, without visible changes to the cell culture potential or gonocyte proportion. In another experiment where testis tissues were maintained under hypothermic conditions, we found that ~25% of testis cells could survive for 6 days if preserved in HypoThermosol-FRS solution (HTS-FRS), without morphological changes. To purify gonocytes, we showed that centrifugation of testis cells using 17% Nycodenz can lead to precipitation of gonocytes in pellets (with a purity of > 80%). We also found that pre-coating tissue culture plates with both fibronectin and poly-D-lysine can result in the negative selection of gonocytes (with a purity of up to 85%). We subsequently showed that further purification of gonocytes (to > 90%) could be achieved by combining the two latter approaches. To prepare recipients for germ cell transplantation, we used local irradiation of piglet testes which reduced testis growth, decreased seminiferous tubule diameters and completely eliminated spermatogenesis at 4 months post-irradiation. Compared with the absence of endogenous spermatogenesis in the control testes, spermatogenesis up to elongating spermatids was observed in the irradiated testes after gonocyte transplantation. In summary, we investigated several critical elements in the study and manipulation of gonocytes in a large animal model.

The studies in this thesis examined piglet gonocyte identification, isolation, purification, preservation and potential for initiation of spermatogenesis after transplantation into irradiated recipient testes. As a first step, we characterized a previously non-described auto-fluorescence in the piglet testis tissue. This auto-fluorescence mainly originated from granules among the testis interstitial cells, and we found that its interference with immuno-fluorescence can be overcome using Sudan black staining. We also showed that porcine gonocytes can be specifically labelled with the lectin Dolichos biflorus agglutinin (DBA). To optimize gonocyte isolation, we found that ~9-fold more live cells could be harvested by enzymatic digestion of testis tissues than with mechanical methods. However, the proportion of gonocytes (~7%) did not differ between the mechanical and enzymatic methods of testis cell isolation. We then developed a novel three-step strategy for isolation of gonocytes by combining enzymatic digestion and vortexing, resulting in a gonocyte proportion of ~40% (~5-fold more than that from conventional methods). For short-term preservation of testis cells, we found that the survival of testis cells under hypothermic conditions was dependent on the cell type, and affected by storage duration, temperature and medium used. More than 80% of live testis cells survived the 6-day hypothermic preservation period in 20% FBS-L15, without visible changes to the cell culture potential or gonocyte proportion. In another experiment where testis tissues were maintained under hypothermic conditions, we found that ~25% of testis cells could survive for 6 days if preserved in HypoThermosol-FRS solution (HTS-FRS), without morphological changes. To purify gonocytes, we showed that centrifugation of testis cells using 17% Nycodenz can lead to precipitation of gonocytes in pellets (with a purity of > 80%). We also found that pre-coating tissue culture plates with both fibronectin and poly-D-lysine can result in the negative selection of gonocytes (with a purity of up to 85%). We subsequently showed that further purification of gonocytes (to > 90%) could be achieved by combining the two latter approaches. To prepare recipients for germ cell transplantation, we used local irradiation of piglet testes which reduced testis growth, decreased seminiferous tubule diameters and completely eliminated spermatogenesis at 4 months post-irradiation. Compared with the absence of endogenous spermatogenesis in the control testes, spermatogenesis up to elongating spermatids was observed in the irradiated testes after gonocyte transplantation. In summary, we investigated several critical elements in the study and manipulation of gonocytes in a large animal model.

Diss. (sammanfattning) Umeå : Umeå universitet, 2009. / Härtill 2 uppsatser. Även tryckt utgåva.

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Primordial follicles are the first small follicles to appear in the mammalian ovary. Women are born with a fixed number of primordial follicles in the ovaries. Once formed, the pool of primordial follicles serves as a source of developing follicles and oocytes. The first aim of this thesis was to investigate the functional role of the intra-oocyte signaling pathways, especially the phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin complex 1 (mTORC1) pathways in the regulation of primordial follicle activation and survival. We found that a primordial follicle remains dormant when the PI3K and mTORC1 signaling in its oocyte is activated to an appropriate level, which is just sufficient to maintain its survival, but not sufficient for its growth initiation. Hyperactivation of either of these signaling pathways causes global activation of the entire pool of primordial follicles leading to the exhaustion of all the follicles in young adulthood in mice. Mammalian oocytes, while growing within the follicles, remain arrested at prophase I of meiosis. Oocytes within the fully-grown antral follicles resume meiosis upon a preovulatory surge of leutinizing hormone (LH), which indicates that LH mediates the resumption of meiosis. The prophase I arrest in the follicle-enclosed oocyte is the result of low maturation promoting factor (MPF) activity, and resumption of meiosis upon the arrival of hormonal signals is mediated by activation of MPF. MPF is a complex of cyclin dependent kinase 1 (Cdk1) and cyclin B1, which is essential and sufficient for entry into mitosis. Although much of the mitotic cell cycle machinery is shared during meiosis, lack of Cdk2  in mice leads to a postnatal loss of all oocytes, indicating that Cdk2 is important for oocyte survival, and probably oocyte meiosis also. There have been conflicting results earlier about the role of Cdk2 in metaphase II arrest of Xenopus  oocytes. Thus the second aim of the thesis was to identify the specific Cdk that is essential for mouse oocyte meiotic maturation. We generated mouse models with oocytespecific deletion of Cdk1  or Cdk2  and studied the specific requirements of Cdk1 and Cdk2 during resumption of oocyte meiosis. We found that only Cdk1 is essential and sufficient for the oocyte meiotic maturation. Cdk1 does not only phosphorylate the meiotic phosphoproteins during meiosis resumption but also phosphorylates and suppresses the downstream protein phosphatase 1, which is essential for protecting the Cdk1 substrates from dephosphorylation.