Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:36Z No. of bitstreams: 5 54.pdf: 760236 bytes, checksum: c96473cb1f96d0ebd32276b921d2f10d (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:26:35Z (GMT) No. of bitstreams: 5 54.pdf: 760236 bytes, checksum: c96473cb1f96d0ebd32276b921d2f10d (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:26:35Z (GMT). No. of bitstreams: 5 54.pdf: 760236 bytes, checksum: c96473cb1f96d0ebd32276b921d2f10d (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / A cultura do crambe (Crambe abyssinica Hochst) tem se destacado no cen?rio nacional pelo seu potencial para produ??o de biodiesel devido ? facilidade de cultivo, qualidade do ?leo e possibilidades de uso dos subprodutos da extra??o do ?leo. Para o estabelecimento da cultura no pa?s ? necess?rio que sejam usadas sementes de qualidade. No entanto, as informa??es sobre metodologias para avalia??o da qualidade de sementes dessa cultura s?o escassas. Dessa forma, objetivou-se adequar as metodologias dos testes de envelhecimento acelerado e condutividade el?trica para avalia??o do vigor de sementes de crambe e investigar a atividade enzim?tica em rela??o ?s diferen?as de vigor. Foram utilizados cinco lotes de sementes da cultivar FMS Brilhante das safras 2008, 2009, 2010 e 2011. Foram realizadas a caracteriza??o morfol?gica de sementes e pl?ntulas e a composi??o centesimal da semente de crambe. Para caracteriza??o do perfil dos lotes realizou-se a determina??o do grau de umidade e os testes de primeira contagem de germina??o, germina??o, ?ndice de velocidade de germina??o, emerg?ncia, estande inicial, ?ndice de velocidade de emerg?ncia e sanidade. Foi tamb?m realizada a an?lise eletrofor?tica das isoenzimas super?xido dismutase, esterase, catalase, ?lcool desidrogenase e malato desidrogenase. Para o teste de envelhecimento acelerado, as sementes foram submetidas ao m?todo tradicional e com solu??o saturada de NaCl, pelos per?odos de envelhecimento de 0; 24; 48; 72 e 96 horas. No teste de condutividade el?trica, as sementes foram submetidas aos per?odos de 2; 4; 6; 8; 10; 12; 14; 16 e 18 horas de embebi??o utilizando-se 25 sementes em 25 mL e 50 mL e 50 sementes em 50 mL e 75 mL. Concluiu-se que ? poss?vel avaliar o vigor de sementes de crambe pelo m?todo tradicional do teste de envelhecimento acelerado a 42 ?C por 96 horas. O teste de condutividade el?trica n?o foi adequado para avalia??o da qualidade fisiol?gica de crambe. Quando associado ? atividade das isoenzimas observou-se que o lote de maior vigor teve maior atividade dos grupos enzim?ticos super?xido dismutase, catalase e esterase. O lote de menor vigor n?o teve atividade das enzimas isocitrato liase e ?lcool desidrogenase. N?o houve altera??o na atividade da isoenzima malato desidrogenase. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Crambe (Crambe abyssinica Hochst) has been highlighted in the national scenery for its potential in producing biodiesel due to its ease of cultivation, quality of the oil and possibilities of use of the oil extraction byproducts. In order to establish the culture in the Country, the use of quality seeds is necessary. However, the information on methodologies for seed quality evaluation for this culture is scarce. Thus, we aimed at adapting the methodologies to the accelerated aging and electric conductivity tests to evaluate the vigor of crambe seeds and investigate the enzymatic activity in relation to the vigor differences. Five seed lots of cultivar FMS Brilhante of the 2008, 2009, 2010 and 2011 harvests were used. We performed the morphologic characterization of seeds and seedlings and the centesimal composition of crambe seeds. For the profile characterization of the lots, we performed the determination of the humidity degree and the tests for first germination count, germination, germination speed index, emergence, initial stand, emergence speed index and sanity. We also performed the electrophoretic analysis of the superoxide dismutase, esterase, catalase, alcohol dehydrogenase and malate dehydrogenase isoenzymes. For the accelerated aging test, the seeds were submitted to the traditional method and with NaCl saturated solution, for the aging periods of 0; 24; 48; 72 and 96 hours. In the electrical conductivity test, the seeds were submitted to the periods of 2; 4; 6; 8; 10; 12; 14; 16 and 18 hours of soaking using 25 seeds in 25 mL and 50 mL, and 50 seeds in 50 mL and 75 mL. We concluded that it is possible to evaluate crambe seed vigor by the traditional method of the accelerated aging test at 42 oC for 96 hours. The electric conductivity test was not adequate for evaluating crambe physiological quality. When associated with isoenzymes activity, we observed that the lot with highest vigor presented the highest activity of the superoxide dismutase, catalase and esterase isoenzymes groups. The lot of lowest vigor did not present activity of the isocitrate liase and alcohol dehydrogenase enzymes. There was no interaction of the activity of the malate dehydrogenase isoenzymes.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:36Z No. of bitstreams: 5 55.pdf: 569820 bytes, checksum: b67e70762aca5d283f8f7ed1df3d96f0 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:29:22Z (GMT) No. of bitstreams: 5 55.pdf: 569820 bytes, checksum: b67e70762aca5d283f8f7ed1df3d96f0 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:29:22Z (GMT). No. of bitstreams: 5 55.pdf: 569820 bytes, checksum: b67e70762aca5d283f8f7ed1df3d96f0 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2011 / O presente estudo teve por objetivo avaliar mudas de Hymenea courbaril L. (jatob?) quanto: (a) ? capacidade competitiva com plantas consortes e esp?cies daninhas referentes a aloca??o de mat?ria seca, ?rea foliar e concentra??o de macronutrientes; (b) ? seletividade aos herbicidas sulfentrazone e glyphosate e; (c) aos efeitos de diferentes doses de sulfentrazone sobre a atividade microbiana de substratos cultivados com as mudas. Os experimentos foram conduzidos em casa-de-vegeta??o, viveiro de produ??o de mudas e em laborat?rio na Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina-MG. No ano de 2009, foram conduzidos dois experimentos sendo os tratamentos compostos pela combina??o de mudas de jatob?, se desenvolvendo isoladamente ou em competi??o com cada uma das seguintes esp?cies Brachiaria humidicola, Brachiaria brizantha, Brachiaria decumbens, Panicum maximum, Cajanus cajan, Canavalia ensiformis e Mucuna aterrima (experimento 1) e Cenchrus echinatus L., Bidens pilosa L., Euphorbia heterophylla L., Solanum americanum Mill e Lolium multiflorum Lam. (experimento 2), mais o cultivo isolado de cada planta daninha e consorte, por 60 dias. Al?m do efeito das plantas nas caracter?sticas de altura e mat?ria seca das plantas de jatob?, avaliou-se o ac?mulo de nitrog?nio, f?sforo e pot?ssio em todas as plantas. Em 2010, tamb?m foram conduzidos dois experimentos sendo os tratamentos compostos por dois herbicidas (glyphosate e sulfentrazone) e cinco doses desses herbicidas (0,00; 0,09; 0,18; 0,35 e 0,70 L ha-1) (experimento 1); cinco doses de sulfentrazone (0,00; 0,04; 0,15; 0,30 e 0,60 L ha-1) e dois tipos de substratos (rizosf?rico e n?o rizosf?rico cultivados com mudas de jatob?) (experimento 2), sendo avaliado o potencial de intoxica??o desses produtos sobre a esp?cie vegetal e a comunidade microbiana rizosf?rica. Observou-se que a competi??o entre as plantas n?o promoveu altera??es na produ??o de mat?ria seca ou ?rea foliar do jatob?. Sobre a conviv?ncia das plantas daninhas com o jatob?, constatou-se efeito positivo no ac?mulo de nutrientes por estas. Os herbicidas estudados apresentaram-se com elevado potencial para uso no controle de plantas daninhas em ?reas de plantios de jatob? em fase inicial de desenvolvimento. Quanto aos indicadores microbiol?gicos, verifica-se que a evolu??o do C-CO2 e o carbono da biomassa microbiana foram sens?veis ? presen?a dos herbicidas podendo constituir ferramentas auxiliares no monitoramento do impacto desses produtos no ambiente. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT The objective of this study was to evaluate Hymenaea courbaril L (jatoba) seedling in relation to: a) the competitive capability with intercropped plants and weed regarding to dry matter allocation, leaf area and macronutrients concentration. b) The selectivity of herbicides sulfentrazone and glyphosate and, c) the effects of different doses of sulfentrazone on microbial activity of substrates cultivated with the seedling. The experiments were conducted in greenhouse, nursery and laboratory of UFVJM. In 2009, two experiments were carried out, being the treatments a combination of jatoba seedling that had developed alone and/or in competition with one of the following species: Brachiaria humidicola, Brachiaria brizantha, Brachiaria decumbens, Panicum maximum, Cajanus cajan, Canavalia ensiformis and Mucuna aterrima (experiment 1) and Cenchrus echinatus L., Bidens pilosa L., Euphorbia heterophylla L., Solanum americanum Mill and Lolium multiflorum Lam. (experiment 2), plus the isolated cultivation of every weed and plant that was intercropped for 60 days. Besides the effects of the weed plants on height and dry matter of jatoba plants, it was assessed the accumulation of nitrogen, phosphorus and potassium in all of plants. In 2010, it is also made two experiments and the treatment was made with two herbicides (glyphosate and sulfentrazone) and five doses of these herbicides (0,00; 0,09; 0,18; 0,35 e 0,70 L ha-1) (experiment 1); five doses of sulfentrazone (0,00; 0,04; 0,15; 0,30 e 0,60 L ha-1) and two types of substrates (rhizospheric and non-rhizospheric cultivated with jatoba seeding) (experiment 2). It was assessed the poisoning potential of these products on vegetal species and the rhizospheric microbial community. It was observed that the competition between the plants did not trigger changes on production of dry matter or leaf area of jatoba. It was verified a positive effect of nutrients accumulation by weed that lived together with jatoba. These herbicides have showed high potential for use on weed control in areas of jatoba planting in initial phase of development. Regarding to microbiological indicators, it was verified that the C-CO2 and microbial biomass carbon evolution was sensitive to the presence of herbicides that be constitute auxiliary tools on monitoring of the impact of these products.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:36Z No. of bitstreams: 5 57.pdf: 589227 bytes, checksum: c5ece0beb9b99077696ceef16ab4aa71 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:31:27Z (GMT) No. of bitstreams: 5 57.pdf: 589227 bytes, checksum: c5ece0beb9b99077696ceef16ab4aa71 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:31:27Z (GMT). No. of bitstreams: 5 57.pdf: 589227 bytes, checksum: c5ece0beb9b99077696ceef16ab4aa71 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2011 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / O presente trabalho teve como objetivo avaliar resposta de diferentes l?minas de irriga??o definidas a medi??o di?ria pela ETo em minijardim clonal para produ??o de mudas de eucalipto h?brido de E. urophylla S.T. Blake e E. grandis W. Hill ex Maiden. O experimento foi conduzido no viveiro da Empresa Sada Bio Energia e Agricultura LTDA, Sete Lagoas (MG), no per?odo de abril a novembro de 2010. No minijardim clonal, adotou-se o delineamento em blocos casualizados, contendo sete l?minas de irriga??o e quatro blocos, totalizando em 28 unidades experimentais. Nos demais setores do viveiro (casa de vegeta??o, casa de sombra e aclimata??o a c?u aberto), o delineamento foi de blocos casualizados, contendo sete l?minas de irriga??o, quatro blocos e dois n?veis de redu??o foliar, totalizando em 56 unidades experimentais. Os tratamentos foram definidos de acordo com a leitura diariamente do Tanque Classe A (T1 = 50% da ETo - freq??ncia ?nica ; T2= 75% da ETo ? duas vezes ao dia; T3 = 100% da ETo ? duas vezes ao dia; T4 = 125% da ETo ? tr?s vezes ao dia; T5 = 150% da ETo ? tr?s vezes ao dia; T6 = 100% da ETo - freq??ncia ?nica e T7- operacional da empresa - l?mina 10,66 mm dia-1, fertirriga??o oito vezes ao dia, durante cinco minutos com uma vaz?o de 0,8 L h-1. A l?mina 1,46 mm dia-1 (T1 - 50% da ETo) ? recomendada para irriga??o de minijardim clonal para produ??o de mudas de eucalipto. Sem redu??o foliar da miniestaca pode ser utilizado na produ??o de mudas de eucalipto. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT This work had as its main objective to evaluate the response from different irrigation water levels, defined the daily measurement through ETo in clonal mini gardens to the hybrid eucalyptus E. urophylla S.T. Blake and E. grandis W. Hill ex Maiden plants production. The experiment was conducted at the Sada Bio Energia e Agricultura Ltda Company, in the city of Sete Lagoas, Minas Gerais state, Brazil, between April and November, 2010. In the mini-clonal garden, it had been adopted the randomized block design, with seven irrigation water levels and four blocks, totaling 28 experimental units. In the other sectors of the nursery (greenhouse, shade house and acclimation to the open air), the design was in randomized blocks with seven irrigation levels, four blocks and two levels of reduction of foliar area, totaling 56 experimental units. The treatments were defined according to the daily reading of the Class A Tank (T1 = 50% of ETo ? single frequency ; T2= 75% of ETo ? twice a day; T3 = 100% of ETo ? twice a day; T4 = 125% of ETo ? three times a day; T5 = 150% of ETo ? three times a day; T6 = 100% of ETo ? single frequency e T7- company?s operational - level 10,66 mm day- fertilizer and irrigation eight times a day for five minutes with a flow rate of 0.8 L h-1. The level 1.46mm a day -1 (T1 ? 50% of ETo) is recommended for irrigation in clonal mini gardens for eucalyptus sprouts production. Without reduction of the mini sprouts foliar area, can be used for the production of eucalyptus plants.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:37Z No. of bitstreams: 5 60.pdf: 417283 bytes, checksum: 6813899edea0a249245bbb9ba9654b3b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:38:37Z (GMT) No. of bitstreams: 5 60.pdf: 417283 bytes, checksum: 6813899edea0a249245bbb9ba9654b3b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:38:37Z (GMT). No. of bitstreams: 5 60.pdf: 417283 bytes, checksum: 6813899edea0a249245bbb9ba9654b3b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Empresa de Pesquisa Agropecu?ria de Minas Gerais (EPAMIG) / O teste LERCAF? consiste na imers?o de sementes de caf? em solu??o de hipoclorito de s?dio. O cloro ativo, princ?pio ativo da solu??o, reage com o endosperma das sementes, identificando regi?es mortas ou lesionadas, colorindo-as de verde escuro. A partir da avalia??o da localiza??o da regi?o colorida, ? poss?vel classificar as sementes como vi?veis ou n?o vi?veis. O teste ? r?pido e de opera??o simples, mas a metodologia necessita ser testada para obter melhor precis?o e exatid?o dos resultados. Objetivou-se com este trabalho, adequar a metodologia do teste LERCAF? na determina??o da viabilidade de sementes de caf? (Coffea arabica L.), al?m de avaliar o perfil isoenzim?tico em sementes submetidas ao teste LERCAF?. Em um primeiro experimento, avaliou-se a efici?ncia do teste LERCAF? na determina??o da viabilidade em sementes de caf? das cultivares Catua? Amarelo IAC 44, Mundo Novo IAC 376-4, Travessia MGS, e Rubi MG 1192, para isso utilizaram-se solu??es de hipoclorito de s?dio com teores de 2,5%; 3,5%; 5,0% e 6% de cloro ativo e os per?odos de imers?o de 2, 3 e 6 horas, a 30 ?C. Observou-se pela caracteriza??o do perfil das cultivares, que a velocidade de germina??o n?o variou entre as cultivares, no entanto houve superioridade na germina??o da cultivar Rubi em rela??o ? Catua? Amarela e Travessia. No entanto, pelo teste LERCAF? foi poss?vel apenas ? separa??o das cultivares em dois n?veis de qualidade, por meio dos tratamentos 2,5% por 3 h, 3,5% por 2 h e 3 h, sendo as cultivares Rubi, Travessia e Mundo Novo de qualidade superior em rela??o a cultivar Catua? Amarelo. Na concentra??o de 2,5% de hipoclorito de s?dio por 2 horas, as sementes n?o apresentaram colora??o esverdeada no endosperma. J? nas concentra??es de 2,5% por 6 horas, 5% e 6% por 2h e 3h foi observada colora??o intensa dificultando a avalia??o das sementes. Na busca da adequa??o da metodologia do teste LERCAF?, foi realizado um segundo experimento, utilizando um lote de sementes de caf? da cultivar Catua? Vermelho IAC 99, neste experimento foi realizado a quantifica??o do teor de cloro ativo da solu??o de hipoclorito de s?dio e posteriormente avaliada a efici?ncia do teste, utilizando-se concentra??es de 1%, 2%, 3%, 4% e 5% de cloro ativo e per?odos de 1, 2, 3, 4 e 5 horas, a 30 ?C, tamb?m foi avaliado o perfil isoenzim?tica para enzimas Esterase (EST), Malato Desidrogenase(MDH), Super?xido Dismutase (SOD), Catalase (CAT) e ?lcool Desidrogenase (ADH). O teste LERCAF? permite a determina??o do potencial fisiol?gico das sementes de caf?, quando se utiliza solu??o de hipoclorito de s?dio quantificada, pelos tratamentos onde as sementes s?o imersas em solu??o com teor de 2% de cloro ativo pelo per?odo de 5 horas e 3% de cloro ativo pelo per?odo de 3 horas, a 30?C. As sementes de caf? submetidas ao teste LERCAF? apresentam altera??es na atividade das enzimas EST, MDH, SOD, CAT e ADH, sendo que a ativa??o ou desativa??o destes sistemas enzim?ticos s?o vari?veis com a concentra??o e tempo de imers?o na solu??o de cloro ativo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT The LERCAF? test consists in the immergence of coffee seeds in sodium hypochlorite solution. The active chloride, active component of the solution, reacts with the endosperm of the seeds, identifying dead or injured regions, staining them dark green. From the colored region location evaluation, it is possible to classify the seeds as viable or non-viable. The test is quick and of simple transaction, however, the methodology needs to be tested in order to obtain better result precision and accuracy. The objective of this work was to adjust the methodology of the LERCAF? test in determining the viability of coffee (Coffea Arabica L.) seeds, in addition to evaluating the isoenzymatic profile of seeds submitted to the LARCAF? test. A first experiment evaluated the efficiency of the LERCAF? test in determining the viability of coffee seeds of cultivars Yellow Catuai IAC 44, Novo Mundo IAC 376-4, Travessia MGS and Rubi MG 1192. In order to do this, we used sodium hypochlorite solutions with active chloride contents of 2.5%, 3.5%, 5.0% and 6.0% and immersion periods of 2, 3 and 6 hours, at 30 oC. By the characterization of the cultivar profiles, we observed that germination speed did not vary between the cultivars, however, there was superiority in cultivar Rubi germination in relation to Yellow Catuai and Travessia. However, by the LERCAF? test, only the separation of the cultivars in two quality levels was possible, with the treatments 2.5% for 3 h, 3.5% for 2 h and 3 h, with cultivars Rubi, Travessia and Mundo Novo of superior quality in relation to Yellow Catuai cultivar. At the concentration of 2.5% of sodium hypochlorite for 2 hours, the seeds did not present greenish coloring on the endosperm. In the concentrations of 2.5% for 6 hours, 5% and 6% for 2 h and 3 h, intense coloration was observed, making seed evaluation difficult. Seeking to adjust the LERCAF? test methodology, a second experiment was conducted, using a lot of coffee seeds of cultivar Red Catuai IAC 99. This experiment quantified the sodium hypochlorite solution?s content of active chloride and, subsequently evaluated the efficiency of the test, using active chloride concentrations of 1%, 2%, 3%, 4% and 5% and the periods of 1,2,3,4 and 5 hours, at 30 oC. The isoenzymatic profile for enzymes Esterase (EST), Malate Dihydrogenase (MDH), Superoxide Dismutase (SOD), Catalase (CAT) and Alcohol Dehydrogenase (ADH) was also evaluated. The LERCAF? test allows the determination of physiological potential of the coffee seeds, when using quantified sodium hypochlorite solution, by the treatments in which the seeds are immersed in solution with 2% active chloride content for the period of 5 hours, and 3% active chloride for the period of 3 hours, at 30 oC. The coffee seeds submitted to the LERCAF? test presented alterations in the activity of enzymes EST, MDH, SOD, CAT and ADH, being that the activation of deactivation of these enzymatic systems vary with the concentration and time of immersion in active chloride solution.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:37Z No. of bitstreams: 5 61.pdf: 450363 bytes, checksum: cd83469eae8b523d2f8e1536e42d83d6 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:40:11Z (GMT) No. of bitstreams: 5 61.pdf: 450363 bytes, checksum: cd83469eae8b523d2f8e1536e42d83d6 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:40:11Z (GMT). No. of bitstreams: 5 61.pdf: 450363 bytes, checksum: cd83469eae8b523d2f8e1536e42d83d6 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / O herbicida glyphosate n?o ? seletivo e de largo espectro de controle de plantas daninhas, seu mecanismo de a??o ocorre com a inibi??o da enzima 5-enolpiruvilchiquimato-3-fosfato sintase (EPSPs), acontecendo o bloqueio da rota do ?cido chiqu?mico, precursor de amino?cidos arom?ticos e de outros metabolitos secund?rios. O objetivo deste trabalho foi avaliar as altera??es nas caracter?sticas fotossint?ticas e do uso eficiente da ?gua por plantas de cafeeiro submetidas ? aplica??o de glyphosate. O experimento foi conduzido em casa de vegeta??o utilizando-se tr?s cultivares de caf? (Coffea arabica): Acai? (MG-6851), Catuca? Amarelo (2 SL) e Top?zio (MG-1190) e tr?s subdoses do glyphosate (0,0; 115,2 e 460,8 g ha-1), em esquema fatorial 3x3, com 4 repeti??es. Os cultivares de caf? se diferiram quanto ? atividade fotossint?tica. Com o aumento das subdoses do herbicida, observou-se maiores consequ?ncias negativas sobre as vari?veis fotossint?ticas. Tais efeitos podem ser atribu?dos aos danos diretos na atividade fotossint?tica ou pelos indiretos, afetando o metabolismo da planta. Com a aplica??o do herbicida, as plantas de cafeeiro apresentaram redu??es de taxa transpirat?ria e condutividade estom?tica, por?m menor efici?ncia do uso da ?gua apenas aos 15 DAA na quarta folha. Os cultivares apresentaram efeitos negativos com a aplica??o das subdoses de glyphosate, quanto a transpira??o e condut?ncia estom?tica. Pode-se concluir que o cultivar Acai? apresentou-se mais tolerante, pois n?o mostrou efeitos prejudiciais na efici?ncia do uso da ?gua. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT The herbicide glyphosate is nonselective and of wide-spectrum weed control; its mechanism of action is the inhibition of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs), blocking shikimic acid route, a precursor of aromatic amino acids and other secondary metabolites. The objective of this study was to evaluate the changes in photosynthetic characteristics and of water use efficiency for coffee plants submitted to glyphosate application. The experiment was conducted in a greenhouse using three varieties of coffee (Coffea Arabica): Acai? (MG-6851), Catuca? Amarelo (2 SL) e Top?zio (MG-1190) and, three subdoses of glyphosate (0,0; 115,2 and 460,8 g ha-1), in a factorial 3x3, with four replicates. The coffee cultivars subjected to reduced rates of glyphosate to differ in terms of photosynthetic activity. With the increase in sub dosage of the herbicide, there have been observed more negative consequences on the photosynthetic variables. These effects can be attributed to the direct damages on photosynthetic activity or to the indirect ones, affecting the metabolism of the plant. With the herbicide application, coffee plants presented reduction of perspiration rate and stomatal conductivity, however less efficiency in water use only at 15 DAA, on the fourth leaf. The cultivars presented negative effects with the application of a sub dosage of glyphosate regarding perspiration and stomatal conductivity. It can be concluded that the cultivar Acai? showed to be more tolerant, as it did not show prejudicial effects in water use efficiency.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:37Z No. of bitstreams: 5 62.pdf: 876239 bytes, checksum: 8956f78b3fdb90e76e402bae0772e928 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:42:59Z (GMT) No. of bitstreams: 5 62.pdf: 876239 bytes, checksum: 8956f78b3fdb90e76e402bae0772e928 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:42:59Z (GMT). No. of bitstreams: 5 62.pdf: 876239 bytes, checksum: 8956f78b3fdb90e76e402bae0772e928 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / A fitorremedia??o, que ? um processo de extra??o de elementos potencialmente t?xicos atrav?s da utiliza??o de plantas, tem se mostrado um m?todo promissor. Objetivou-se com este trabalho avaliar em gram?neas forrageiras a absor??o, o ac?mulo e a toler?ncia ao chumbo (Pb), cultivadas em solo Latossolo Vermelho-Amarelo distr?fico e solu??o nutritiva, e avaliar a disponibilidade de Pb pelos m?todos de extra??o Mehlich 1, DTPA pH 7,3, USEPA 3051 e 3052 nas amostras de solos. Instalaram-se os experimentos em condi??es de casa de vegeta??o do Departamento de Agronomia da Faculdade de Ci?ncias Agr?rias da UFVJM, Diamantina (MG). Foram avaliadas as forrageiras: Panicum maximum cv. Aruana e cv. Tanz?nia; Brachiaria decumbens cv. Basilisk; Brachiaria brizantha cv. Xara?s e cv. Marandu, e doses de Pb em solu??o nutritiva de 0, 40, 120, e 360 mg L-1 e em solo de 0, 45, 90 e 270 mg kg-1. O delineamento experimental foi inteiramente casualizado, com quatro repeti??es e per?odo experimental de 90 dias, em ambos os experimentos. Determinaram-se a massa seca e os teores de Pb na parte a?rea, coleto e ra?zes das forrageiras. Os conte?dos de Pb foram calculados com base nos teores e nas produ??es de massa seca em cada parte da planta. Para aferi??o do teor de Pb no solo foram utilizados os extratores Mehlich 1, DTPA pH 7,3 para teor dispon?vel, teor semitotal - USEPA 3051 e teor total - USEPA 3052. As forrageiras estudadas reduziram o crescimento com aumento das doses de Pb aplicadas, tanto na solu??o nutritiva quanto no solo, configurando a fitotoxidade do Pb nessas plantas. Por?m, a suscetibilidade foi diferenciada entre os experimentos, sendo que, na solu??o nutritiva, as cultivares Marandu e Basilisk foram mais promissoras para a fitorremedia??o, devido a menor influ?ncia do Pb em seus crescimentos relativos, enquanto no solo a cv. Basilisk apresentou maior toler?ncia ao elemento t?xico em rela??o ?s demais forrageiras. Em ambos os experimentos, o teor de Pb nas forrageiras aumentou com as doses crescentes de Pb. Entretanto, a presen?a de Pb nas duas condi??es de cultivo fez com que o ac?mulo e ?ndice de transloca??o nas diferentes partes da planta apresentassem respostas diferenciadas para as forrageiras. N?o houve correla??o entre o crescimento das forrageiras com a avalia??o do Pb no solo pelos m?todos de extra??o Mehlich e DTPA pH 7,3, indicando a inefici?ncia destes quanto a fitodisponibiliza??o do Pb. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Phytoremediation, which is a process of extracting potentially toxic elements through the use of plants, has shown to be a promising method. The objective of this project was to evaluate in forage grasses the absorption, accumulation and tolerance to lead (Pb) in dystrophic Red-Yellow Latosol soil and nutrient solution, and to evaluate the availability of Pb through extraction methods Mehlich 1, DTPA pH 7.3, USEPA 3051 and 3052 in samples soil. The experiments were settled under conditions of a greenhouse in the Department of Agronomy of the College of Agricultural Sciences of the Universidade Federal dos Vales do Jequitinhonha e Mucuri, (UFVJM), Diamantina (MG). The forages Panicum maximum cv. Aruana and cv. Tanz?nia and Brachiaria decumbens cv. Basilisk, Brachiaria brizantha cv. Xara?s and cv. Marandu and four rates of Pb in nutrient solution of 0, 40, 120, and 360 mg L-1 and in soil of 0, 45, 90 e 270 mg kg-1, have been evaluated. The experimental design was completely randomized with four replications, and a trial period of 90 days in both experiments. The dry weight and concentration of Pb in the shoot, in the stem base and roots of forages were assessed. The contents of Pb were calculated based on the amounts and on the production of dry mass in each part of the plant. In the case of the soil experiment, to measure the levels of Pb were used Mehlich 1, DTPA pH 7,3 for available content; semi total content - USEPA 3051 and total content - USEPA 3052. The forages studied reduced growth when increasing rates of Pb were applied, both in the nutrient solution and on the soil, setting the phytotoxicity of Pb in these plants. However the susceptibility was different for the experiments, because in the nutrient solution, the Marandu and Basilisk forages proved to be promising for phytoremediation because of the lowering influence of Pb for the last one mentioned and the higher accumulation of metal for the first one, while on the soil the forage Basilisk did better than the other, proving to be more tolerant to the toxic element. In both experiments (soil and nutrient solution) the Pb content in the forage increased due to increasing rates of Pb. However, the presence of Pb in the cultivation conditions has made the accumulation and translocation index in different parts of the plants to respond differently to the forages. There was not correlation between the forage growth with the admeasurement of Pb in soil by extraction methods DTPA pH 7,3 e Mehlich 1, proving the inefficiency of the same for the phytoavailability of Pb.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:37Z No. of bitstreams: 5 64.pdf: 928043 bytes, checksum: 9eff581b95268e24883923008dfea1b5 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:46:09Z (GMT) No. of bitstreams: 5 64.pdf: 928043 bytes, checksum: 9eff581b95268e24883923008dfea1b5 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:46:09Z (GMT). No. of bitstreams: 5 64.pdf: 928043 bytes, checksum: 9eff581b95268e24883923008dfea1b5 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2012 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Minist?rio do Desenvolvimento Agr?rio/Secretaria de Agricultura Familiar / O m?dio vale do Jequitinhonha apresenta condi??es clim?ticas adversas, tendendo para a semiaridez, com precipita??es anuais abaixo de 1.000 mm, demandando estrat?gias de conviv?ncia com essas condi??es. Os solos agr?colas nessas regi?es tropicais, por estarem expostos aos fen?menos clim?ticos, t?rmicos e h?dricos, necessitam de prote??o cont?nua, alcan?ada atrav?s da cobertura, viva ou morta, proporcionada principalmente por leguminosas herb?ceas perenes utilizadas na aduba??o verde. Este trabalho foi desenvolvido com o objetivo de avaliar o comportamento e as potencialidades de leguminosas herb?ceas perenes para uso como aduba??o verde, em recupera??o ao per?odo de seca, e avaliar a produ??o do quiabeiro em cultivo sobre a cobertura viva dessas leguminosas, sob manejo org?nico, na regi?o do m?dio vale do Jequitinhonha/MG. Dois experimentos foram conduzidos: o primeiro com as leguminosas cudzu tropical (Pueraria phaseoloides), calopog?nio (Calopogonium mucunoides), amendoim forrageiro (Arachis pintoi), soja perene (Glycine wightii), estilosantes campo grande (Stylosanthes capitata, Stylosanthes macrocephala) e com plantas espont?neas (controle); e o segundo com o quiabeiro cultivado em cons?rcio com essas leguminosas. O delineamento experimental utilizado nos dois experimentos foi o de blocos, nesse caso com quatro repeti??es. Observou-se que no primeiro experimento o cudzu e a soja perene restabeleceram-se na ?rea, mais influenciados pela rebrota do que pelo ressemeio; o amendoim forrageiro e o estilosantes restabeleceram-se pelos dois m?todos propagativos citados; e o calopog?nio se restabeleceu praticamente por ressemeio. As esp?cies cudzu tropical, calopog?nio, amendoim forrageiro e soja perene se destacaram para a cobertura do solo. O uso das leguminosas como cobertura permanente promoveu inibi??o e mudan?as na composi??o das esp?cies de plantas espont?neas ao longo do tempo, com destaque para o cudzu. Todas as leguminosas proporcionaram menor temperatura do solo em rela??o ao controle, com destaque para o cudzu e amendoim forrageiro. O calopog?nio se destacou entre as leguminosas com maior capacidade de reten??o da umidade do solo. O uso de leguminosas perenes como calopog?nio, cuduz tropical e soja perene, pode contribuir para o incremento de N, e a ciclagem dos macronutrientes, al?m do aumento da mat?ria org?nica sobre o solo, por meio do material senescente. Independentemente do tratamento, foram encontrados maiores valores de P, K, Mg, SB, pH e mat?ria org?nica nos primeiros 5 cm de profundidade. Os tratamentos calopog?nio, cudzu e soja perene se destacaram, para o teor de K, Mg, SB, H+Al e T em todas as profundidades do solo, com o controle tamb?m se destacando para o teor de K. Em todas as profundidades, o solo sob cudzu revelou o menor valor de pH. Amendoim forrageiro, calopog?nio e soja perene se destacaram para o teor de P na camada de 10 a 20 cm de profundidade do solo. O calopog?nio apresentou os maiores teores de mat?ria org?nica em todas as profundidades, e independentemente do tratamento, na medida em que se aumenta a profundidade do solo, observam-se valores decrescentes para o teor de mat?ria org?nica. O controle, calopog?nio, cudzu e estilosantes apresentaram o maior valor de carbono da mat?ria org?nica leve. No segundo experimento observou-se que ap?s estiagem, com in?cio do restabelecimento, cudzu tropical, soja perene e amendoim forrageiro, demonstraram consider?vel potencial de ac?mulo de mat?ria seca na parte a?rea. Ap?s o corte, o calopog?nio e o amendoim forrageiro restabeleceram-se bem, principalmente atrav?s de germina??o, proporcionada pelo banco de sementes depositado no solo e tamb?m por rebrota, no caso do amendoim forrageiro. O cudzu e a soja perene promoveram menor presen?a de plantas espont?neas. Amendoim forrageiro e calopog?nio se destacaram em proporcionar menor temperatura do solo e todas as leguminosas promoveram maior reten??o de umidade do solo, com exce??o do estilosantes, quando comparados com o controle. O quiabeiro cultivado sobre soja perene e cudzu tropical apresentou maiores alturas. A aduba??o verde com soja perene, estilosantes e cudzu tropical, proporcionou aumento no n?mero e produtividade de frutos de quiabeiro por colheita, por somat?rio de colheitas, e por classe. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The m?dio vale do Jequitinhonha is severely punished by adverse climate conditions, tending to the semiarid, with annual rainfall below 1.000 mm, then it is required strategies for dealing with these conditions. Soils in these tropical regions need continuous protection because they are exposed to extreme climate, temperature and water. Bad consequences can be minimized by alive or dead coverage which came mainly from perennial herbaceous leguminous used for green manure. This study aimed to evaluate the performance and potential of perennial herbaceous leguminous to be used as green manure on recovery at dry period, and the production of okra cultivated on these leguminous living coverage, under organic management in the region of m?dio vale do Jequitinhonha/MG. Two experiments were conducted: the first one with tropical kudzu (Pueraria phaseoloides), calopo (Calopogonium mucunoides), forage peanut (Arachis pintoi), perennial soybeans (Glycine wightii), stylosanthes (Stylosanthes capitata, Stylosanthes macrocephala) and control group; and in the second one, okra was intercropped with these leguminous. A randomized block design was used with four replicates. It was observed that in the first experiment, the perennial soybean and kudzu had re-established themselves in the area, more influenced by re-growth than by re-seeding. Forage peanut and stylosanthes were restored by the two propagation methods mentioned, and calopo was restored by re-seeding. The tropical kudzu, calopo, forage peanut and perennial soybean species stood out on soil coverage. The use of leguminous plants as permanent coverage promoted inhibition and changes in the weed species composition of the species over time, emphasing kudzu. All leguminous plants provided lower soil temperature as compared to the control group, especially the kudzu and forage peanut. The calopo stood out among the leguminous plants with a greater capacity to retain soil humidity. The use of these perennial leguminous may contribute to the increase of N, and the cycling of macronutrients besides increasing organic matter on the soil through senescent material. Regardless of the treatment, higher values of P, K, Mg, SB, pH and organic matter were found in the first 5 cm depth. The calopo, kudzu and perennial soybean treatments stood out for the content of K, Mg, SB, T and H + Al in all soil depths, with the control group also standing out for the content of K. In all depths, the soil under kudzu showed the lowest pH value. Peanut forage, calopo and perennial soybean stood out for P content from 10 to 20 cm of soil depth. The calopo showed the highest levels of organic matter at all depths, regardling of the treatment. As soil depth increases, values of organic matter content decreases. The control group, calopo, kudzu and stylosanthes showed the greatest amount of carbon from light organic matter. In the second experiment it was observed that after dry time, beginning the restoration, kudzu tropical, perennial soybean and forage peanut, showed considerable potential for dry matter accumulation in shoots. After cutting, forage peanut and calopo had a great recoverage, mainly through germination, provided by the seed bank in the soil and also in the case of forage peanut re-growth. The kudzu and perennial soybean promoted lower re-infestation of weeds. Calopo and the forage peanut were better in providing lower soil temperature and all leguminous plants promoted a greater retention of soil humidity, except for stylosanthes, when compared to control group. The okra grown on soybeans and tropical kudzu presented higher heights. Green manure with perennial soybean, Kudzu and stylosanthes increased the number and the productivity of okra by harvest, by the sum of harvests, and by class.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:37Z No. of bitstreams: 5 65.pdf: 1478609 bytes, checksum: 3267d3f03abffcb32e457026cf296e53 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:47:24Z (GMT) No. of bitstreams: 5 65.pdf: 1478609 bytes, checksum: 3267d3f03abffcb32e457026cf296e53 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:47:24Z (GMT). No. of bitstreams: 5 65.pdf: 1478609 bytes, checksum: 3267d3f03abffcb32e457026cf296e53 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2012 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / A fam?lia Leguminosae ? composta por cerca de 727 g?neros e 19.325 esp?cies e, ? bem representada na Serra do Espinha?o, considerada Reserva da Biosfera pela UNESCO, portadora de alto grau de endemismos e esp?cies raras, que vem sofrendo com a perda de habitats pela a??o antr?pica. Muitas esp?cies de leguminosas apresentam potencial para recupera??o de ?reas degradadas, uma vez que possuem associa??o com bact?rias fixadoras de nitrog?nio, principalmente as do g?nero Chamaecrista. Contudo, existem poucas informa??es a respeito das esp?cies desse g?nero. E, considerando a import?ncia biol?gica dos campos rupestres, a ecol?gica das leguminosas e a lacuna de conhecimento que se tem sobre as esp?cies da flora na Cadeia do Espinha?o no Planalto de Diamantina, MG, esse estudo teve como objetivo conhecer os aspectos relacionados ? fenologia e germina??o de sementes de Chamaecrista debilis (Vogel) Irwin e Barneby. As observa??es fenol?gicas foram realizadas em ?reas de campos rupestres no campus da Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina-MG. Os testes de germina??o foram conduzidos no Laborat?rio de Sementes da UFVJM. Foram marcados e monitorados 30 indiv?duos da esp?cie, onde avaliou-se quinzenalmente, de abril de 2010 a mar?o de 2011, as seguintes fenofases: caducifolia, brota??o, flora??o e dispers?o. Verificou-se que a brota??o e flora??o, estiveram associadas com a esta??o ?mida enquanto a dispers?o e a queda de folhas com a esta??o seca. Para avaliar a germina??o foi efetuado um teste de germina??o com 12 tratamentos: testemunha, escarifica??o com lixa d??gua n.80, imers?o em ?gua a 100oC por 5, 10, 15 , 30 e 60 segundos e imers?o em ?cido sulf?rico concentrado por 5, 10, 15, 30 e 60 segundos. Foram avaliadas as porcentagens de germina??o das sementes, o ?ndice de velocidade de germina??o e do teor de ?gua das sementes de Chamaecrista debilis. Utilizou-se o delineamento inteiramente casualizado com quatro repeti??es, e as m?dias foram comparadas pelo teste de Scott-Knott, a 5% de probabilidade. Os dados indicaram que a sazonalidade clim?tica da regi?o ? determinadora dos padr?es fenol?gicos em Chamaecrista debilis. Para a germina??o verificou-se que as sementes apresentaram teor de ?gua de 11,71%. Os tratamentos com ?gua a 1000C a 5, 10 e 15 segundos e ?cido sulf?rico a 15 segundos foram os mais eficientes em promover a germina??o. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The family Leguminosae comprises about 727 genera and 19,325 species, and is well represented in the Espinha?o considered a Biosphere Reserve by UNESCO, the bearer of a high degree of endemic and rare species, which has suffered the loss of habitats by human activity. Many species of legumes have potential for recovery of degraded areas, as they have an association with nitrogen fixing bacteria, especially those of the genus Chamaecrista. However, little information exists about the species of this genus. And considering the biological importance of the stony fields, the leguminous plants and ecological knowledge gap that exists about the species of flora in the Espinha?o of the Diamantina Plateau, Minas Gerais (MG), Brazil, this study aimed to understand the aspects related to phenology and germination seeds of Chamaecrista debilis (Vogel) Irwin and Barneby. The phenological observations were carried out in areas of rocky fields on the campus of University of the Valleys of Jequitinhonha and Mucuri (UFVJM), Diamantina, MG. Germination tests were conducted at the Laboratory of Seeds of UFVJM. Were marked and monitored 30 individuals of the species, which we assessed biweekly from April 2010 to March 2011, the following phenophases: shedding, budding, flowering and seed dispersal. It was found that the budding and flowering, were associated with the wet season while the dispersion and fall of leaves in the dry season. To evaluate the germination, a germination test conducted with 12 treatments: control, scarification with sandpaper n.80, in water at 1000C for 5, 10, 15, 30 and 60 seconds and immersion in concentrated sulfuric acid for 5, 10, 15, 30 and 60 seconds. We evaluated the germination of seeds, the germination speed index and water content of seeds of Chamaecrista debilis. We used a completely randomized design with four replications and means were compared by Scott-Knott test at 5% probability. The data indicated that the climate in the region is the determinant of phenological patterns in Chamaecrista debilis. For germination was found that the seeds had a water content of 11.71%. The treatment with water at 1000C to 5, 10 and 15 seconds and 15/2 sulfuric acid were more effective in promoting germination.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:38Z No. of bitstreams: 5 9.pdf: 426387 bytes, checksum: 43d0eba992e9c2abb675c693cabf2496 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:56:25Z (GMT) No. of bitstreams: 5 9.pdf: 426387 bytes, checksum: 43d0eba992e9c2abb675c693cabf2496 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:56:26Z (GMT). No. of bitstreams: 5 9.pdf: 426387 bytes, checksum: 43d0eba992e9c2abb675c693cabf2496 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2010 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Este trabalho teve como objetivo comparar em tr?s cultivos a produtividade, componentes de rendimento, atributos qu?micos do solo e a nutri??o do feijoeiro em quatro sistemas de preparo do solo de um Neossolo Quartzar?nico. O primeiro cultivo do feijoeiro foi realizado de abril a junho de 2006, o segundo, de novembro de 2006 a janeiro de 2007, e o terceiro de dezembro de 2008 a fevereiro de 2009. O delineamento experimental utilizado foi o de blocos casualizados no esquema de experimento em faixas, com cinco repeti??es. Os tratamentos foram os seguintes preparos do solo: arado de disco, arado de aiveca, grade pesada e plantio direto com o uso de dessecante na vegeta??o. Os sistemas de preparo influenciaram nos atributos qu?micos do solo, na produtividade e nutri??o do feijoeiro nas condi??es de Campo Limpo na Serra do Espinha?o Meridional. O plantio direto e grade pesada s?o os sistemas de preparo do solo mais adequados para a produ??o do feijoeiro, quando cultivado sob um Neossolo Quartzar?nico. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010. / ABSTRACT This study aimed to compare three crop yields, yield components, soil chemical properties and nutrition of common bean in four tillage systems on soil conditions in Quartzarenic Neossol. The first crop of the common bean was in April-June, 2006, the second was from November, 2006 to January, 2007 and the third was from December, 2008 to February, 2009. The experimental design was randomized blocks of the scheme in split plot, with five replicates. The treatments were the following soil tillage: disk plow, moldboard plow, heavy offset disk harrow and no-tillage with the use of desiccant in vegetation. The tillage systems influenced the chemical properties of soil, yield and nutrition of bean plants under field conditions in the Campo Limpo in Serra of Espinha?o Meridional. No-till and heavy offset disk harrow are the most suitable soil tillage for the bean productivity when the growth is in a sandy soil.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-09T19:14:10Z No. of bitstreams: 1 2009 - Evandro Silva Pereira Costa.pdf: 2009437 bytes, checksum: cf64a3e8f7e3e004841a52c65f4c6626 (MD5) / Made available in DSpace on 2017-02-09T19:14:10Z (GMT). No. of bitstreams: 1 2009 - Evandro Silva Pereira Costa.pdf: 2009437 bytes, checksum: cf64a3e8f7e3e004841a52c65f4c6626 (MD5) Previous issue date: 2009-09-21 / CNPq / Defining methods of quantification of tomato late blight, caused by Phytophthora infestans, is important to many researches about strategies of management of the disease. The aim of this present study was to assess and to validate the diagrammatic grading keys - Simplified, James-modified and Broad - and to determinate the possible correlations between the measures of severity and plants? physiological and nutritional state and biomass accumulation. Two simultaneous experiments were carried out: one in greenhouse and the other in field conditions. Two cultivars were studied in greenhouses, Super Sweet and ?Perinha ?gua Branca?, a resistant and a susceptible to late blight genotypes, respectively. The plants were inoculated or not with a sporangia suspension, and submitted to ten evaluations: 1, 2, 3, 4, 6, 7, 9, 10, 17 and 24 days after inoculation. The avaliations were performed in the fifth oldest leaf, and eighth and eleventh leaves, by visual estimation of severity, using three diagrammatic grading keys, Simplified, Broad and James-modified, but also measuring photosynthetic potential (Fv/Fm), by chlorophyll ?a? fluorescence emission. In field conditions, four cultivars were evaluated ? ?Perinha ?gua Branca?, Super Sweet, Santa Clara and Carmen F1. Periodically, late blight severity was quantified using the same three grading keys in leaves of different plant parts, at 51, 62, 74, 94 and 102 days after transplant (DAT). At the day following each severity disease evaluation, one plant was harvested per plot to measure the accumulation of biomass and macronutrients of different plant organs. Fruit yield was determined by summing fruits harvested between 68 and 114 DAT. In greenhouse conditions, the estimative of late blight severity with the three diagrammatic grading keys proved to be adequate to the disease?s quantification, enabling the discrimination between inoculation effects and differences between the cultivars in four and in nine days after inoculation, respectively, which were confirmed by measures of the photosynthetic potential of the leaves. In field conditions, the diagrammatic grading keys Simplified and Broad were more adequate to tomato late blight quantification. Quantifications in the youngest leaves, in the medium and superior third part of the plant, were the most representative of the disease progress and also discriminated the cultivars regarding disease resistance. Negative correlations were observed between severity, estimated with the three grading keys considering the whole plant and its medium third part, and leaf dry mass and leaf concentrations of K and P. The cultivar ?Perinha ?gua Branca? showed the lowest stem and leaf dry mass at the beginning of plant evaluations, and also late fruit yield, but had the higher stem and leaf mass and at the end of the experiment. The cultivar Perinha ?gua Branca and the hybrid Carmen F1 had higher commercial fruit yield. Based in the results, it is recommended the use of Simplified diagrammatic grading key and evaluations in the medium or superior tomato third to quantify the late blight disease. / A defini??o de m?todos de quantifica??o da requeima no tomateiro, causada por Phytophthora infestans, ? importante para v?rias pesquisas sobre estrat?gias de manejo da doen?a. Esse trabalho teve como objetivo aferir e validar as escalas diagram?ticas, Simplificada, James-modificada e Detalhada e determinar as poss?veis correla??es entre as medidas de severidade e o estado fisiol?gico e nutricional da planta e o ac?mulo de biomassa. Realizaram-se dois experimentos simult?neos, um sob condi??es de ambiente protegido e o outro sob condi??es de campo. Em casa de vegeta??o, utilizaram-se duas cultivares de tomate cereja, ?Perinha ?gua Branca? e Super Sweet, suscet?vel e resistente ? requeima, respectivamente. As plantas foram inoculadas ou n?o com suspens?o de espor?ngios de P. infestans e em seguida, avaliadas aos 1, 2, 3, 4, 6, 7, 9, 10, 17 e 24 dias ap?s a inocula??o. As avalia??es foram feitas na quinta, oitava e d?cima primeira folha, contadas do ?pice para a base, usando as tr?s escalas acima citadas e medi??es do potencial fotossint?tico (Fv/Fm) pela emiss?o da clorofila ?a?. No campo, utilizaram-se quatro cultivares, ?Perinha ?gua Branca?, Super Sweet, Santa Clara e Carmen F1. Periodicamente, quantificou-se a severidade da requeima utilizando-se as mesmas escalas acima citadas em folhas de diferentes partes da planta, aos 51, 62, 74, 94 e 102 dias ap?s o transplante (DAT). No dia seguinte a cada avalia??o de severidade, foi coletada uma planta por parcela para mensurar o ac?mulo de biomassa e de macronutrientes nos diferentes ?rg?os da planta. A produ??o de frutos foi determinada pelo somat?rio das colheitas realizadas entre 68 e 114 DAT. Em condi??es de casa de vegeta??o, a estimativa da severidade da requeima com as tr?s escalas se mostrou adequada para quantifica??o da doen?a tendo permitido discriminar efeito de inocula??o e diferen?as entre as cultivares aos quatro e nove dias ap?s a inocula??o, respectivamente, que foram confirmadas pelas medidas do potencial fotossint?tico das folhas. Em condi??es de campo, as escalas Simplificada e Detalhada, foram mais adequadas para a quantifica??o da requeima do tomateiro. Quantifica??es feitas nas folhas mais jovens, no ter?o m?dio e superior da planta, foram as que melhor representaram o progresso da doen?a e discriminaram as cultivares quanto a resist?ncia ? doen?a. Observaram-se correla??es negativas entre a severidade, estimada com as tr?s escalas, considerando a planta inteira e o ter?o m?dio, e a massa seca de folhas e os teores foliares de K e P nas quatro cultivares. A cultivar ?Perinha ?gua Branca? apresentou menor massa seca de caule e de folha no in?cio das avalia??es e produ??o de frutos mais tardia, por?m ao final do ciclo destacou-se pela maior massa seca de caule e de folha. A cultivar ?Perinha ?gua Branca? e o h?brido Carmen F1 apresentaram maior produ??o de frutos comerciais. Com base nos resultados pode-se recomendar o uso da escala simplificada e avalia??es no te?o m?dio ou superior do tomateiro para quantifica??o da requeima.