Two trial were conducted to determine the possible effects of season, protein supplementation, age and birth status on the reproduction rate (ovulation rate and rate of twinning) of ewes. In Trial 1 the weight, age and birth status if ewes were recorded. 144 ewes were randomly allocated in two treatment groups (urea and mix protein group) synchronised, mated and the number of corpora lutea, foetuses observed, lambs born per ewe and the mass of the ewe after lambing were also recorded. Lambing status or the 1-year-old (0.993 ± 0.316) and 2-year-old (1.233 ± 0.134) ewes were lower (p < 0.05) than that of the 6- year-old ewes (1.897 ± 0.248). The lambing status and the number of corpora lutea of the single born ewes (1.179 ± 0.131; 1.274 ± 0.138) were lower (p < 0.0001) than that of the twin born ewes (1.614 ± 0.139; 1.782 ± 0.147), within the urea treatment. In Trial 2, 75 ewes were randomly allocated in four treatment groups (raw lupins, cooked lupins, cottonseed oil-cake and Fescue grass), synchronised and the number of corpora lutea were recorded. The weight, age and birth status of the ewes were also recorded. The number of corpora lutea from the cooked lupin group (1.815 ± 0.184) was significantly higher than that from the cottonseed oilcake group (1.048 ± 0.209), within the twin born ewe group. It was concluded that season, protein supplementation, age and birth status influenced the reproduction rate of ewes. Copyright / Dissertation (Msc)--University of Pretoria, 2009. / Animal and Wildlife Sciences / unrestricted

Ninety Merino ewes, divided into three equal groups, were kept on natural highveld grazing for 42 weeks. Group M received a mineral supplement continuously, averaging 28 g per day. The other groups received commercial protein supplements, group RDP a mainly rumen degradable supplement and group RUP, a mainly rumen undegradable supplement. These supplements had crude protein (CP) levels of 29% and 28% respectively and were supplied at strategic times during the reproductive cycle, at 250 g per ewe per day for 14 days before mating, at 350 g per ewe per day for 42 days, starting 21 days before lambing and at 500 g per ewe per day for 56 days, starting 21 days after lambing. Grazing was randomized to minimize differences in nutrition and parasite challenge, and had an average CP of 8.8%. Lambing rates were: RUP 96%, RDP 89% and M 76%. Lamb survival rates at 11 and 17 weeks post lambing were 75%&63% for RUP, 64%&57% for RDP and 55% and 48% for M respectively (P< 0.05). Wool production parameters were similar for all groups, as were mean faecal egg counts: 685 (RUP), 371 (RDP) and 465 (M). Body weights, body condition scores and FAMACHA scores were also similar for all three groups. Income per ewe, calculated at 11 and 17 weeks post lambing, was highest for RUP at R147.80&R132.87, lowest for M at R117.86&R111.13, and in between for RDP (R129.85&R121.38). However, the gross margin was the highest for M at both points (R114.35&R107.77) compared to RUP (R70.43&R54.93 – P < 0.03&P < 0.008 respectively), as well as RDP (R82.96&R74.12). Strategic supplementation with protein improved performance but the additional income was not sufficient to cover feed costs under prevailing conditions and neither supplement could therefore be economically justified. / Dissertation (MMedVet)--University of Pretoria, 2002. / Production Animal Studies / unrestricted

Made available in DSpace on 2015-03-26T13:54:49Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1125500 bytes, checksum: 600a602e4ac83076fa059b11cbb583f5 (MD5) Previous issue date: 2008-07-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The scope of this work was to evaluate the use of different sulfur sources in protein supplements for bovines. The experiments were carried out at the Unit of Teaching, Research and Extension on Beef Cattle and Laboratory of Animal Health in the Department of Animal Science at the Federal University of Viçosa, MG, Brazil. In the first experiment, crossbred calves were fed with Brachiaria dictyoneura grass hay, with different sulfur sources in the protein supplement: 70S elemental sulfur; 98S elemental sulfur; calcium sulfate (hydrated gypsum); calcium sulfate (anhydrous gypsum) and ammonium sulfate. The calf response to the different sulfur sources was evaluated by means of the nutrient intake and apparent digestibility, ruminal pH and N-NH3, amino acid profile in the abomasal digesta, fiber degradability and particle flow-rate. An 11:1 nitrogen:sulfur ratio was used. Five Holstein/Zebu male calves fistulated in the rumen and abomasum were used distributed in a 5 x 5 Latin square. The experiment had five 16-day periods, in which the first ten days were used as the adaptation period and the other days were used for collecting the data. The scope of the second experiment was to evaluate the &#8220;in vitro&#8221; microbial protein production regarding the different ratios of calcium sulfate and elemental sulfur in the ruminal liquid of a beef cattle calf at growing stage and a lactating cow. The different sulfur sources in the supplement did not affect (P>0.05) the intakes of hay dry matter and protein supplement, crude protein (CP), neutral detergent fiber corrected for ash an protein (NDFap), organic matter (OM), nonfiber carbohydrate (NFC), ether extract (EE) and total digestible nutrients (TDN), digestibility coefficients of NDFap and CP, ruminal pH and the ruminal ammonia concentration. In this study, the degradation profiles of NDF achieved were interpreted according to the solid transit kinetics parameters model and estimations. The data was adjusted to different double-compartment models (G1G1, G2G1, G3G1, G4G1, G5G1 and G6G1). The models G2G1, G3G1, G5G1, G4G1 and G3G1 showed to be, respectively, more efficient on determining the estimations of the following treatments: 70S elemental sulfur, 98S elemental sulfur, calcium sulfate (hydrated gypsum), calcium sulfate (anhydrous gypsum) and ammonium sulfate. The sulfur source used influenced slowly the ruminal fiber degradation. The concentration of available amino acids in the abomasal digesta (g/kg DM, where DM is dry matter) did not change (P>0.05) in the evaluated diets. The availability of the amino acids evaluated in the abomasal digesta did not vary and their profile did not depend on the sulfur sources found in the diets. In the second experiment, differences in the rates of &#8220;in vitro&#8221; microbial protein for the evaluated ratios were not found (P>0.05). The sulfur sources evaluated may be used to formulate beef cattle supplements, as long as an 11:1 nitrogen:sulfur ratio is used. / Objetivou-se avaliar a utilização de diferentes fontes de enxofre em suplementos proteinados para bovinos. Os experimentos foram realizados na Unidade de Ensino, Pesquisa e Extensão em Gado de Corte e no Laboratório de Nutrição Animal do Departamento de Zootecnia, na Universidade Federal de Viçosa. No primeiro experimento avaliou-se a resposta de novilhos mestiços alimentados com feno de capim Brachiaria dictyoneura, variando-se a fonte de enxofre no suplemento proteinado, sendo: enxofre elementar 70S; enxofre elementar 98S; sulfato de cálcio (Gesso hidratado); sulfato de cálcio (Gesso anidro) e sulfato de amônio, sobre o consumo e a digestibilidade aparente dos nutrientes, pH e amônia (N-NH3) ruminal, perfil de aminoácidos na digesta abomasal, degradabilidade da fibra e taxa de passagem de partículas, mantendo-se uma relação nitrogênio:enxofre de 11:1. Utilizou-se cinco novilhos mestiços Holandês/Zebu fistulados no rúmen e no abomaso, distribuídos em um quadrado latino 5 X 5. O experimento constou de cinco períodos com duração de 16 dias cada um, sendo os dez primeiros para adaptação e os demais para a coleta de dados. No segundo experimento objetivou-se avaliar a produção de proteína microbiana &#8220;in vitro&#8221; em função de diferentes relações entre sulfato de cálcio e enxofre elementar em líquido ruminal proveniente de um novilho de corte em crescimento e de uma vaca em lactação. As diferentes fontes de enxofre no suplemento não afetaram (P>0,05) os consumos de matéria seca do feno e do suplemento proteinado, proteína bruta (PB), fibra em detergente neutro corrigido para cinzas e proteína (FDNcp), matéria orgânica (MO), carboidratos não fibrosos (CNF), extrato etéreo (EE) e nutrientes digestíveis totais (NDT), os coeficientes de digestibilidade da FDNcp e da PB, o pH ruminal e a concentração de amônia no rúmen. Neste estudo os perfis de degradação da FDN obtidos foram interpretados utilizando o modelo e as estimativas dos parâmetros da cinética de passagem de partículas ajustando os dados a diferentes modelos bicompartimentais (G1G1, G2G1, G3G1, G4G1, G5G1 e G6G1). Os modelos G2G1, G3G1, G5G1, G4G1 e G3G1 mostraram-se mais eficientes na determinação das estimativas dos parâmetros de cinética de passagem de partículas para os tratamentos: enxofre elementar 70S, enxofre elementar 98S, sulfato de cálcio (Gesso hidratado), sulfato de cálcio (Gesso anidro) e sulfato de amônio, respectivamente. A degradação ruminal da fibra sofreu pouca influência da fonte de enxofre utilizada. As concentrações dos aminoácidos disponíveis na digesta abomasal (g/kg MS) não diferiram (P>0,05) entre as dietas avaliadas. A disponibilidade dos aminoácidos avaliados na digesta abomasal não variou e seu perfil não dependeu das fontes de enxofre presente nas dietas. No segundo experimento não foram verificadas diferenças nos teores de proteína microbiana &#8220;in vitro&#8221; para as relações avaliadas (P>0,05). As fontes de enxofre avaliadas podem ser utilizadas na formulação de suplementos para bovinos de corte, mantendo-se a relação nitrogênio:enxofre de 11:1.

As práticas de manejo das pastagens tropicais recomendadas para utilização intensiva da planta permitem ao animal colher material de boa qualidade. O objetivo do trabalho foi avaliar se a proteína bruta (PB) proveniente do capim seria suficiente para atender as exigências de vacas leiteiras no terço médio da lactação, tornando desnecessária a utilização de suplementos protéicos no concentrado. Buscou-se também a caracterização da PB do capim e a adequação protéica da dieta, visando otimizar a eficiência de utilização do nitrogênio. No primeiro estudo, 33 vacas holandesas e mestiças (HPBxJersey), no terço médio da lactação, produzindo em média 20 L dia-1, foram agrupadas em 11 blocos, de acordo com composição racial, dias em lactação e produção de leite e receberam suplemento concentrado com três níveis de PB durante 76 dias. Os concentrados continham milho moído fino, minerais, vitaminas e os três níveis de proteína (T1: 8,7%; T2: 13,4%; T3: 18,1% MS) foram obtidos substituindo-se parte do milho por farelo de soja. O capim elefante foi adubado com 50 kg N ha-1 por pastejo e apresentou teor médio de PB de 18,5%. As produções de leite e de leite corrigido para 3,5% de gordura, os teores e produções de gordura, proteína e caseína no leite, a síntese de proteína microbiana e o consumo de matéria seca (CMS) não foram influenciados (P>0,05) pelos teores de PB dos concentrados. A concentração de nitrogênio uréico no leite aumentou linearmente (P<0,01) com o aumento dos teores de PB dos concentrados. A excreção de N pela urina foi maior (P<0,05) para os concentrados contendo farelo de soja (T2 e T3) em relação ao concentrado contendo apenas milho (T1). No segundo estudo, 4 vacas holandesas secas, fistuladas no rúmen, foram distribuídas em um quadrado latino 4x4 e submetidas aos mesmos tratamentos do primeiro estudo, acrescidos de um quarto concentrado (T4) com 13,4% de PB, tendo uréia como fonte de nitrogênio. O CMS foi maior para o T4 (P<0,05), sugerindo deficiência de PDR nos outros tratamentos. Entretanto, essa hipótese não foi confirmada pelos parâmetros ruminais (pH, e ácidos graxos de cadeia curta) e síntese microbiana, que não sofreram efeito dos teores e fontes de PB no concentrado. A concentração de N amoniacal no rúmen foi menor (P<0.05) para T1 do que para os demais tratamentos. A excreção total de N no ambiente acompanhou numericamente o teor de PB dos concentrados, mas as diferenças não foram detectadas pela análise estatística. O terceiro estudo utilizou os mesmos animais e tratamentos do segundo para avaliar a cinética de degradação ruminal da MS, PB e FDN do capim por meio de ensaio in situ. Os concentrados protéicos (T2, T3 e T4) não melhoraram a degradação ruminal do capim, em relação ao concentrado energético (T1), confirmando que a proteína do pasto foi suficiente para maximizar a fermentação ruminal. O fornecimento de farelo de soja no concentrado de vacas em terço médio de lactação, mantidas em pastagens tropicais manejadas intensivamente, não resultou em nenhum benefício produtivo e aumentou a excreção de N no ambiente. / Grazing management techniques are improving the quality of the forage consumed by grazing dairy cows. The objective of this study was to determine if forage crude protein (CP) would be sufficient to achieve the nutritional requirements of midlactating dairy cows, making unnecessary the use of protein supplements on the concentrate. It also aimed to characterize the forage CP fractions and to balance the diet protein content to optimize nitrogen use efficiency. In the first trial, 33 Holstein and crossbred (Holstein X Jersey) mid-lactating dairy cows, producing 20 L d-1, were grouped in 11 blocks, according to breed, days in milk and milk yield and were fed three levels of CP on the concentrate supplement for 76 days. Concentrates contained fine ground corn, minerals, vitamins and three CP levels (T1:8.7, T2:13.4 and T3:18.1% DM) that were achieved by replacing corn with soybean meal. Pasture was fertilized with 50 kg N ha-1 after each grazing cycle and averaged 18.5% CP. There was no difference (P>0.05) in milk yield, fat corrected milk, fat, protein and casein content and yield, microbial synthesis and dry matter (DM) intake among treatments. Milk urea nitrogen increased linearly (P<0.01) as the concentrate CP content increased. The N urine excretion was greater (P<0.05) with the soybean meal concentrates (T2 and T3) than with the corn concentrate (T1). In the second trial, 4 rumen cannulated Holstein dry cows were used in a 4x4 Latin square and assigned to the same treatments used in the first study plus a fourth concentrate (T4) with 13,4% CP, containing urea instead of soybean meal as the N source. Dry matter intake was greater for T4 (P<0.05), suggesting a PDR deficiency in the other treatments. However, this hypothesis was not confirmed by ruminal parameters (pH and short chain fatty acids concentration) neither by microbial synthesis, that were not affected by the levels or sources of CP on the concentrate. Ruminal ammonia N content numerically increased as the concentrate CP content increased, but no differences were detected by the statistic analysis. The third study used the same animals and treatments of the second study to evaluate the ruminal kinetics of DM, CP and NDF of Elephant grass by an in situ assay. Protein concentrates (T2, T3 and T4) didnt improve forage ruminal degradation in relation to the energy concentrate (T1), confirming that the forage protein content was sufficient to optimize ruminal fermentation. The inclusion of soybean meal in the concentrate of midlactating dairy cows grazing intensively managed tropical grasses didnt result in productive advantage and increased the N excretion to the environment.

Fontes alternativas de alimentos vem sendo estudadas em substituição à farinha de peixe na aquicultura, podendo acarretar diferença na incorporação de carbono e nitrogênio decorrentes da disponibilidade e do valor nutricional da fonte. Três experimentos tiveram o objetivo de quantificar a participação das diferentes fontes protéicas nos tecidos de juvenis e pós-larvas através da composição isotópica de C e N e sua influência no crescimento. Além disso, a digestibilidade aparente das dietas contendo mistura de plantas C3 e C4 foi estudada usando o 13C nas fezes como traçador. As dietas consistiram de quatro ingredientes protéicos. Farelo de soja FS, farelo de coco FC, farinha de vísceras de aves FVA e farinha de peixe FP que compuseram cinco combinações diferentes: FS100; FS60FC40; FS60FVA40; FS60FP40; FS19F10FP10FVA11. No primeiro experimento duzentos juvenis de tilápia com peso médio de 3,4 ± 0,20 g foram distribuídos em 20 gaiolas flutuantes e após serem alimentados com as dietas experimentais, foram coletados amostras aos 0, 22, 44, 66 e 88 dias para estimar a contribuição de C e N das dietas no tecido. Foi observada diferença significativa (P<0,05) entre peso final (PF), ganho em peso (GP), conversão alimentar (CA), rendimento de filé (RFL), taxa de eficiência protéica (TEP) e fator de condição (FC). No entanto, não observou diferença entre o consumo alimentar (CO), peso do filé (PF) e índice de perfil (IPERF). Para o experimento dois, trezentas pós-larvas de 20 dias com peso médio inicial de 0,07 ± 0,020 g, foram distribuídas aleatoriamente, perfazendo cada unidade experimental com quinze animais. Foram realizadas coletas aos 0, 6, 12, 18, 24 e 30 dias para C e N. Neste experimento foi demonstrada diferença significativa (P<0,05) entre as variáveis PF, GP, CO, CA e TEP. Para o comprimento total e padrão; IPERF e FC n(Pão foi observado diferença (P>0,05). Juvenis e pós-larvas atingiram o equilíbrio isotópico de 13C e 15N com suas respectivas dietas, permitindo assim que estes sejam usados para estimar a quantificação de nitrogênio e carbono, com excessão para a dieta FS60FC40. Em ambos os experimentos, houve aumento percentual de ganho de peso em juvenis e pós-larvas, sendo observada maior porcentagem de nitrogênio no músculo e carcaça total pelas dietas com farinha de peixe FS60FP40 e farinha de vísceras de aves FS60FVA40. Posteriormente, para o experimento de digestibilidade, 2500 juvenis com peso médio de 5,0 ± 1,0 g foram utilizados para determinação do coeficiente de digestibilidade aparente CDA,% de componentes de origem C3 e C4 contidos nas dietas experimentais. Os valores de CDA apresentaram valores inferiores para os componentes de origem C3 em relação aos de origem C4. A avaliação dos isótopos estáveis do C e do N nas dietas permitiu estimatimar a contribuição entre as diferentes fontes, o que sugeriu que a ingestão de nutrientes está relacionados com a qualidade da dieta. Verificou-se que fontes de proteína, quando combinadas, podem apresentar resultados superiores do que quando usada sozinha na composição. / Alternative sources of food have been studied in substitution for fishmeal in aquaculture, and it may result in a difference in incorporation of carbon and nitrogen, depending on the nutritional value and availability of the source. Two experiments were designed to quantify the participation of different protein sources in the muscle tissue in juvenile and post larvae through isotopic composition and its influence on growth and body composition. In addition, the apparent digestibility of feedingstuffs containing mixture of C3 and C4 plants has been studied using 13C as tracer. The experiments were conducted in a random design with five treatments and four repetitions. Diets consisted of by four sources of protein ingredients. soybean meal SBM, coconut meal CM, poultry product meal PPM, meal and fish meal FM, composed five different combinations of diets: SBM100 SBM60CM40 SBM60PPM40 SBM60FM40 SBM19CM10FM10PPM11. In the first experiment, it used 200 juveniles with an average weight of 3.4 ± 0.20 g, distributed in 20 vinyl cages. Fish collections were held after being fed the experimental diets for 0, 22, 28, 38, 44, 66 and 88 days. In this assay, it was observed significant difference (P<0.05) for final weight, weight gain, feed conversion, fillet yield, protein efficiency ratio and condition factorHowever, there was no difference between food consumption, weight and content of the fillet profile. For the experiment two, 300 post-larvae, 20 days old and with average weight starting at 0.07 ± 0.020g, were randomly distributed in the five treatments, making each experimental unit with twenty animals. Six fish collections were held at 0, 6, 12, 18, 24 and 30 days. This assay also showed significant difference (P<0.05) for final weight, weight gain, food intake, feed conversion and protein efficiency ratio. It was observed that juvenile and post larvae did reach the isotopic equilibrium of 13C and 15N with their own diets, thus allowing it to be used for estimating the quantification of nitrogen and carbon incorporated into fish tissue, with exception for the SBM60CM40 diet. The higher percentage of nitrogen in the muscle and carcass were obtained by total diets with fish meal SBM60FM40 and poultry product meal SBM60PPM40To test the digestibility of the diets, 2,500 juvenile with average weight 5.0 ± 1.0 g were used for determination of apparent digestibility coefficient ADC, % of source components C3 and C4 contained in the experimental diets. The values of CDA constituents of experimental diets of fingerling showed lower values for source components C3 in respect of C4 origin. The evaluation of stable isotopes of carbon and nitrogen contained in protein ingredients permitted the estimation of contributions among the different sources, which suggests that the intakes of nutrients are related to the higher quality of the diet. It was noted that protein sources when combined can present superior results when used alone in the composition. The contribution of different protein sources in feed of Nile Tilapia, through the natural variation of stable isotopes of carbon and nitrogen, proved promising.

Recent research suggests that adding protein (PRO) to a carbohydrate (CHO) supplement can have substantial benefits for endurance exercise performance and recovery beyond that of CHO alone. CHO+PRO supplements are often commercially available formulations consisting of carbohydrates (dextrose, maltodextrin) and whey protein. The effects of a supplement containing moderate protein and a low-CHO mixture on endurance performance has not been investigated. Also, the effects of CHO+PRO supplementation in the form of a natural food, flavored milk, on measures of recovery from acute endurance exercise, as well as on chronic aerobic exercise training adaptations, have not been characterized. Therefore, in this series of four studies, the effects of CHO+PRO supplementation on the following areas of endurance exercise performance, recovery, and adaptation are investigated: acute endurance exercise performance, inflammatory and muscle damage markers, muscle glycogen resynthesis, activation of signaling proteins involved in the initiation of protein synthesis and degradation, subsequent endurance exercise performance, and chronic aerobic training adaptations (maximal oxygen consumption, oxidative enzyme activity, body composition, immune cell levels, and inflammatory markers). Study 1 demonstrated that a supplement containing a low-CHO mixture plus moderate protein significantly improved aerobic endurance when cycling at or below the ventilatory threshold, despite containing 50% less CHO and 30% fewer calories relative to a higher CHO beverage. Study 2 demonstrated that CHO+PRO supplementation in the form of chocolate milk (CM) is an effective post-exercise supplement that can improve subsequent performance and provide a greater intracellular signaling stimulus for protein synthesis compared to CHO and placebo. Study 3 found that post-exercise CM supplementation during 4.5 wks of aerobic exercise training improves the magnitude of cardiovascular adaptations more effectively than isocaloric CHO or placebo, while the fourth study demonstrated that post-exercise CM supplementation during 4.5 wks of aerobic training improves body composition more effectively than isocaloric CHO or placebo. The fourth study also demonstrated that 4.5 wks of training does not appear to perturb resting immune cell concentrations or markers of inflammation and muscle damage. Taken together, the results of this research series suggest that CHO+PRO supplementation extends endurance performance, improves recovery, and increases training adaptations more effectively than CHO or placebo. / text

Fontes alternativas de alimentos vem sendo estudadas em substituição à farinha de peixe na aquicultura, podendo acarretar diferença na incorporação de carbono e nitrogênio decorrentes da disponibilidade e do valor nutricional da fonte. Três experimentos tiveram o objetivo de quantificar a participação das diferentes fontes protéicas nos tecidos de juvenis e pós-larvas através da composição isotópica de C e N e sua influência no crescimento. Além disso, a digestibilidade aparente das dietas contendo mistura de plantas C3 e C4 foi estudada usando o 13C nas fezes como traçador. As dietas consistiram de quatro ingredientes protéicos. Farelo de soja FS, farelo de coco FC, farinha de vísceras de aves FVA e farinha de peixe FP que compuseram cinco combinações diferentes: FS100; FS60FC40; FS60FVA40; FS60FP40; FS19F10FP10FVA11. No primeiro experimento duzentos juvenis de tilápia com peso médio de 3,4 ± 0,20 g foram distribuídos em 20 gaiolas flutuantes e após serem alimentados com as dietas experimentais, foram coletados amostras aos 0, 22, 44, 66 e 88 dias para estimar a contribuição de C e N das dietas no tecido. Foi observada diferença significativa (P<0,05) entre peso final (PF), ganho em peso (GP), conversão alimentar (CA), rendimento de filé (RFL), taxa de eficiência protéica (TEP) e fator de condição (FC). No entanto, não observou diferença entre o consumo alimentar (CO), peso do filé (PF) e índice de perfil (IPERF). Para o experimento dois, trezentas pós-larvas de 20 dias com peso médio inicial de 0,07 ± 0,020 g, foram distribuídas aleatoriamente, perfazendo cada unidade experimental com quinze animais. Foram realizadas coletas aos 0, 6, 12, 18, 24 e 30 dias para C e N. Neste experimento foi demonstrada diferença significativa (P<0,05) entre as variáveis PF, GP, CO, CA e TEP. Para o comprimento total e padrão; IPERF e FC n(Pão foi observado diferença (P>0,05). Juvenis e pós-larvas atingiram o equilíbrio isotópico de 13C e 15N com suas respectivas dietas, permitindo assim que estes sejam usados para estimar a quantificação de nitrogênio e carbono, com excessão para a dieta FS60FC40. Em ambos os experimentos, houve aumento percentual de ganho de peso em juvenis e pós-larvas, sendo observada maior porcentagem de nitrogênio no músculo e carcaça total pelas dietas com farinha de peixe FS60FP40 e farinha de vísceras de aves FS60FVA40. Posteriormente, para o experimento de digestibilidade, 2500 juvenis com peso médio de 5,0 ± 1,0 g foram utilizados para determinação do coeficiente de digestibilidade aparente CDA,% de componentes de origem C3 e C4 contidos nas dietas experimentais. Os valores de CDA apresentaram valores inferiores para os componentes de origem C3 em relação aos de origem C4. A avaliação dos isótopos estáveis do C e do N nas dietas permitiu estimatimar a contribuição entre as diferentes fontes, o que sugeriu que a ingestão de nutrientes está relacionados com a qualidade da dieta. Verificou-se que fontes de proteína, quando combinadas, podem apresentar resultados superiores do que quando usada sozinha na composição. / Alternative sources of food have been studied in substitution for fishmeal in aquaculture, and it may result in a difference in incorporation of carbon and nitrogen, depending on the nutritional value and availability of the source. Two experiments were designed to quantify the participation of different protein sources in the muscle tissue in juvenile and post larvae through isotopic composition and its influence on growth and body composition. In addition, the apparent digestibility of feedingstuffs containing mixture of C3 and C4 plants has been studied using 13C as tracer. The experiments were conducted in a random design with five treatments and four repetitions. Diets consisted of by four sources of protein ingredients. soybean meal SBM, coconut meal CM, poultry product meal PPM, meal and fish meal FM, composed five different combinations of diets: SBM100 SBM60CM40 SBM60PPM40 SBM60FM40 SBM19CM10FM10PPM11. In the first experiment, it used 200 juveniles with an average weight of 3.4 ± 0.20 g, distributed in 20 vinyl cages. Fish collections were held after being fed the experimental diets for 0, 22, 28, 38, 44, 66 and 88 days. In this assay, it was observed significant difference (P<0.05) for final weight, weight gain, feed conversion, fillet yield, protein efficiency ratio and condition factorHowever, there was no difference between food consumption, weight and content of the fillet profile. For the experiment two, 300 post-larvae, 20 days old and with average weight starting at 0.07 ± 0.020g, were randomly distributed in the five treatments, making each experimental unit with twenty animals. Six fish collections were held at 0, 6, 12, 18, 24 and 30 days. This assay also showed significant difference (P<0.05) for final weight, weight gain, food intake, feed conversion and protein efficiency ratio. It was observed that juvenile and post larvae did reach the isotopic equilibrium of 13C and 15N with their own diets, thus allowing it to be used for estimating the quantification of nitrogen and carbon incorporated into fish tissue, with exception for the SBM60CM40 diet. The higher percentage of nitrogen in the muscle and carcass were obtained by total diets with fish meal SBM60FM40 and poultry product meal SBM60PPM40To test the digestibility of the diets, 2,500 juvenile with average weight 5.0 ± 1.0 g were used for determination of apparent digestibility coefficient ADC, % of source components C3 and C4 contained in the experimental diets. The values of CDA constituents of experimental diets of fingerling showed lower values for source components C3 in respect of C4 origin. The evaluation of stable isotopes of carbon and nitrogen contained in protein ingredients permitted the estimation of contributions among the different sources, which suggests that the intakes of nutrients are related to the higher quality of the diet. It was noted that protein sources when combined can present superior results when used alone in the composition. The contribution of different protein sources in feed of Nile Tilapia, through the natural variation of stable isotopes of carbon and nitrogen, proved promising.

As práticas de manejo das pastagens tropicais recomendadas para utilização intensiva da planta permitem ao animal colher material de boa qualidade. O objetivo do trabalho foi avaliar se a proteína bruta (PB) proveniente do capim seria suficiente para atender as exigências de vacas leiteiras no terço médio da lactação, tornando desnecessária a utilização de suplementos protéicos no concentrado. Buscou-se também a caracterização da PB do capim e a adequação protéica da dieta, visando otimizar a eficiência de utilização do nitrogênio. No primeiro estudo, 33 vacas holandesas e mestiças (HPBxJersey), no terço médio da lactação, produzindo em média 20 L dia-1, foram agrupadas em 11 blocos, de acordo com composição racial, dias em lactação e produção de leite e receberam suplemento concentrado com três níveis de PB durante 76 dias. Os concentrados continham milho moído fino, minerais, vitaminas e os três níveis de proteína (T1: 8,7%; T2: 13,4%; T3: 18,1% MS) foram obtidos substituindo-se parte do milho por farelo de soja. O capim elefante foi adubado com 50 kg N ha-1 por pastejo e apresentou teor médio de PB de 18,5%. As produções de leite e de leite corrigido para 3,5% de gordura, os teores e produções de gordura, proteína e caseína no leite, a síntese de proteína microbiana e o consumo de matéria seca (CMS) não foram influenciados (P>0,05) pelos teores de PB dos concentrados. A concentração de nitrogênio uréico no leite aumentou linearmente (P<0,01) com o aumento dos teores de PB dos concentrados. A excreção de N pela urina foi maior (P<0,05) para os concentrados contendo farelo de soja (T2 e T3) em relação ao concentrado contendo apenas milho (T1). No segundo estudo, 4 vacas holandesas secas, fistuladas no rúmen, foram distribuídas em um quadrado latino 4x4 e submetidas aos mesmos tratamentos do primeiro estudo, acrescidos de um quarto concentrado (T4) com 13,4% de PB, tendo uréia como fonte de nitrogênio. O CMS foi maior para o T4 (P<0,05), sugerindo deficiência de PDR nos outros tratamentos. Entretanto, essa hipótese não foi confirmada pelos parâmetros ruminais (pH, e ácidos graxos de cadeia curta) e síntese microbiana, que não sofreram efeito dos teores e fontes de PB no concentrado. A concentração de N amoniacal no rúmen foi menor (P<0.05) para T1 do que para os demais tratamentos. A excreção total de N no ambiente acompanhou numericamente o teor de PB dos concentrados, mas as diferenças não foram detectadas pela análise estatística. O terceiro estudo utilizou os mesmos animais e tratamentos do segundo para avaliar a cinética de degradação ruminal da MS, PB e FDN do capim por meio de ensaio in situ. Os concentrados protéicos (T2, T3 e T4) não melhoraram a degradação ruminal do capim, em relação ao concentrado energético (T1), confirmando que a proteína do pasto foi suficiente para maximizar a fermentação ruminal. O fornecimento de farelo de soja no concentrado de vacas em terço médio de lactação, mantidas em pastagens tropicais manejadas intensivamente, não resultou em nenhum benefício produtivo e aumentou a excreção de N no ambiente. / Grazing management techniques are improving the quality of the forage consumed by grazing dairy cows. The objective of this study was to determine if forage crude protein (CP) would be sufficient to achieve the nutritional requirements of midlactating dairy cows, making unnecessary the use of protein supplements on the concentrate. It also aimed to characterize the forage CP fractions and to balance the diet protein content to optimize nitrogen use efficiency. In the first trial, 33 Holstein and crossbred (Holstein X Jersey) mid-lactating dairy cows, producing 20 L d-1, were grouped in 11 blocks, according to breed, days in milk and milk yield and were fed three levels of CP on the concentrate supplement for 76 days. Concentrates contained fine ground corn, minerals, vitamins and three CP levels (T1:8.7, T2:13.4 and T3:18.1% DM) that were achieved by replacing corn with soybean meal. Pasture was fertilized with 50 kg N ha-1 after each grazing cycle and averaged 18.5% CP. There was no difference (P>0.05) in milk yield, fat corrected milk, fat, protein and casein content and yield, microbial synthesis and dry matter (DM) intake among treatments. Milk urea nitrogen increased linearly (P<0.01) as the concentrate CP content increased. The N urine excretion was greater (P<0.05) with the soybean meal concentrates (T2 and T3) than with the corn concentrate (T1). In the second trial, 4 rumen cannulated Holstein dry cows were used in a 4x4 Latin square and assigned to the same treatments used in the first study plus a fourth concentrate (T4) with 13,4% CP, containing urea instead of soybean meal as the N source. Dry matter intake was greater for T4 (P<0.05), suggesting a PDR deficiency in the other treatments. However, this hypothesis was not confirmed by ruminal parameters (pH and short chain fatty acids concentration) neither by microbial synthesis, that were not affected by the levels or sources of CP on the concentrate. Ruminal ammonia N content numerically increased as the concentrate CP content increased, but no differences were detected by the statistic analysis. The third study used the same animals and treatments of the second study to evaluate the ruminal kinetics of DM, CP and NDF of Elephant grass by an in situ assay. Protein concentrates (T2, T3 and T4) didnt improve forage ruminal degradation in relation to the energy concentrate (T1), confirming that the forage protein content was sufficient to optimize ruminal fermentation. The inclusion of soybean meal in the concentrate of midlactating dairy cows grazing intensively managed tropical grasses didnt result in productive advantage and increased the N excretion to the environment.

El presente trabajo tiene como objetivo principal la elaboración y planificación correspondiente de las diferentes tareas y actividades base para iniciar la producción y comercialización de un complemento proteico en polvo especializado para cada deporte o carga física correspondiente, asimismo, será la de comprobar que la idea de negocio es rentable. Actualmente, se pueden encontrar diferentes complementos proteicos en polvo de diferentes marcas con beneficios relativamente similares. El objetivo de estos se basa en la mejoría del estado físico del individuo quien las consume. Sin embargo, se puede apreciar que estas proteínas contienen una composición regular. Por lo que, su composición no se adecua a diferentes cantidades de ejercicio o cargas físicas. Por este motivo, nuestro producto tiene como objetivo principal adecuarse a las diferentes cantidades de carga física de los diferentes tipos de consumidores, clasificándose en deportes o actividades de bajo consumo calórico como de alto consumo calórico de igual manera. Cabe mencionar, que nuestro producto está dirigido a hombres y mujeres cuyo rango de edad se encuentre entre los 18 a 45 años y que hagan deporte regularmente. Para demostrar que la idea de negocio será rentable, se realizaron diferentes análisis de factores internos y externos que pudieran beneficiar o perjudicar a nuestro proyecto. En adición a esto, se realizó el cálculo del valor actual neto proyectado del negocio para comprobar que este generará ganancias en el futuro. / The main objective of the present work is the elaboration and corresponding planning of the different tasks and activities that are the base to start the production and commercialization of a specialized protein powder supplement for each sport or corresponding physical load, as well as to demonstrate that the business idea is profitable. Nowadays, we can find different protein powder supplements from different brands with relatively similar benefits. This objective is based on the improvement of the physical state of the individual who consumes them. However, we can appreciate that these proteins contain a regular composition. Thus, their composition is not adapted to different amounts of exercise or physical loads. For this reason, our product has, as the main objective, to adapt to the different amounts of physical load of the different consumers, being classified in sports or activities of low caloric consumption as of high caloric consumption in the same way. It is worth mentioning that our product is aimed at those men and women whose age is between 18 and 45 years old and who do sports regularly. In order to demonstrate that our business idea will be profitable, different analyses of internal and external factors that could benefit or harm our project were carried out. In addition, we calculated the projected net present value of the business, to prove that it will generate profits in the future. / Trabajo de investigación

The aim of the diploma thesis was to determine, which nutritional supplements are selected by athletes of strength and endurance sports branches and what is the reason for their option. The theoretical part characterizes the 8 selected sports sector from point of view of the sport performance structure. There is also mentioned the issue of nutritional supplements, their division, legislation or general recommendations for the use in sport. The last chapter deals with the requirements for the use of nutritional supplements in different periods with respect to the branche of sport. Research part has specifically determined the nutritional strategy for athletes in the field of mentioned food supplements at strength and endurance-oriented sports disciplines by using the questionnaire. The research is supported by an interview with an expert nutritionist and sports through collaboration with the staff of the Centre for Sports of the Ministry of Interior