The purple sandpipers (Calidris maritima) are the most common waders in the high arctic archipelago of Svalbard, Norway. There they have to cope with a very short summer season and high metabolic costs of migrating far north and breeding in an arctic environment. The food on land is usually scarce, whereas there are rich feeding grounds in the littoral zone, such as in the intertidal zone of river flats. These feeding grounds are though only available to the purple sandpipers during low tide and as long as the estuary is not covered by sea ice. One of these intertidal flats was used as the fieldwork area in this study. To study when the birds are coming to this intertidal flat for feeding, a count study was performed during the entire stay of the purple sandpipers in Svalbard in summer 2010. Point counts were performed at low tide during 118 different days. Additionally, point counts were performed at twenty days during the six hours of the entire low tide period, to study when during the tidal cycle most sandpipers were feeding at the estuary. Most sandpipers were counted at the intertidal flat at the beginning of June with the highest number, 921 individuals, on 8th June. When the tundra was free of snow and the birds could start breeding, numbers where rapidly declining with very few sandpipers left in the estuary in July and the first part of August. From the end of August numbers were increasing again with a second but lower peak in the end of September and beginning of October. By the end of October all sandpipers had left the estuary. The study on the appearance of purple sandpipers at the estuary at the different periods of low tide showed that there were significantly more sandpipers between low tide and half an hour later than at the rest of the low tide period. This might be due to better access to their prey at that time. This knowledge could be used in future studies aiming at recording the maximum numbers. The result of the phenologic study could be included in a long term monitoring to see if the numbers and the timing of purple sandpipers are stable in this area or not: Are the peak numbers differing significantly? Is the timing of the arrival, the stay on the tundra and the timing of leaving the archipelago in the fall changing? Long-term monitoring would be especially interesting in the view of possible influences of the climate change on the purple sandpipers. Rising sea level as a result of the climate change would change the morphology of the estuaries and thereby influence the food resources available for sandpipers.

Purple nonsulfur bacteria are a group of extraordinary metabolic diverse bacteria. They can grow photoautotrophically, photoheterotrophically , chemoheterotrophically or chemoautotrophically. Under various conditions, they can enjoy exceptional flexibility within each of these modes of metabolism. Due to the special physical characteristics properties, they had attracted scientist¡¦s attention in resent years. These bacteria are widely distributed in nature such as lakes, water ponds, coastal lagoons or high concentration organic waste lagoons. Lotus Pond, located in northern Kaohsiung City, is a serious eutrophied artificial lake. Because of receiving sufficient light and having been polluted by significant amounts of soluble organic matter, the ecology of the lake is suitable for the growth of purple nonsulfur bacteria. In the study, the lake water and sediments by using a Winograsdsky column, we successfully isolated 16 strains bacteria from the Lotus Pond. We also amplified the 16S-rDNA fragments of these strains by PCR and sequenced these PCR products, then aligned these sequences with the data of GeneBank. We affirmed that the 16 isolated strains belong to purple nonsulfur bacteria. From phylogenetic analysis, these 16 strains belong to the following three groups of bacteria: Rhodopseudomonas palustris, Rubrivivax gelatinosus, and Rhodobacter sphaeroides. Characteristic studies of these strains, we found that all isolated strains are Gram negative bacteria and contain bacteriochlorophyll a. The strains that belong to R. palustris and R. sphaeroides group can use several different types of short chain organic acid as their carbon source and have denitrification ability. However, only the strains belong to R. palustris group are able to use the aromatic compound benzoate. From salt tolerant studies, we found the strains in R. sphaeroides group can grow well in 3% NaCl, and both R. palustris and R. gelatinosus group can only grow in 1% NaCl.

This thesis examines the influence of nutritional phosphate (Pi) deprivation on extracellular proteins secreted by the model plant Arabidopsis thaliana. Initial studies compared the secretome of Pi-sufficient (+Pi) versus Pi-deficient (-Pi) Arabidopsis cell cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationship between mRNA levels and relative amounts of selected secretome proteins was assessed. The results indicate that transcriptional control is but one of many factors contributing to Arabidopsis Pi starvation responses and highlight the importance of parallel biochemical and proteomic studies of –Pi plants. Three purple acid phosphatase (APase) isoforms were fully purified from the culture media of –Pi Arabidopsis cells and identified as AtPAP12 (At2g27190) and two AtPAP26 (At5g34850) glycoforms. As each purple APase exhibited broad substrate specificities and pH-activity profiles, it is hypothesized that their combined activities facilitate Pi scavenging from soil-localized organophosphates during nutritional Pi deprivation. AtPAP26 is dual-targeted during Pi stress since an earlier report demonstrated that it is also the principal intracellular (vacuolar) APase upregulated by -Pi Arabidopsis. The results indicate that differential glycosylation influences AtPAP26’s substrate specificity and subcellular targeting. An atpap26 T-DNA insertional mutant lacking AtPAP26 transcripts and immunoreactive AtPAP26 polypeptides exhibited: (i) 9- and 5-fold lower shoot and root APase activity, respectively, which did not change in response to Pi starvation, (ii) a 40% reduction in secreted APase activity during Pi deprivation, (iii) 35 and 50% reductions in free and total Pi concentration, respectively, in shoots of –Pi plants, and (iv) impaired shoot and root development when subjected to Pi deficiency. By contrast, no deleterious influence of AtPAP26 loss of function was apparent in +Pi plants. The results establish a firm role for AtPAP26 in the acclimation of Arabidopsis to Pi deficiency. The identification and functional characterization of secreted proteins upregulated by –Pi Arabidopsis is relevant to applied efforts to engineer Pi-efficient transgenic plants, needed to minimize the input of expensive, unsustainable, and polluting Pi fertilizers in crop production. / Thesis (Ph.D, Biology) -- Queen's University, 2010-04-28 17:20:46.892

Upregulation of intracellular and secreted acid phosphatases (APases) is a universal response of orthophosphate-starved (-Pi) plants. APases hydrolize Pi from a broad spectrum of phosphomonoesters at an acidic pH. Plant APases belong to a relatively large multigene family whose specific functions in Pi metabolism are poorly understood. This study focuses on the identification and characterization of cell wall (CW) localized purple acid APases (PAPs) upregulated by -Pi Arabidopsis thaliana. Three glycosylated PAP isozymes secreted into the CW of -Pi Arabidopsis suspension cells were purified and identified by peptide mass fingerprinting using mass spectrometry (MALDI-TOF MS) and N-terminal microsequencing as AtPAP12 (At2g27190; subunit size 60-kDa), AtPAP25 (At4g36350; subunit size 55-kDa) and AtPAP26 (At5g34850; subunit size 55-kDa). Both AtPAP12 and AtPAP26 were previously shown to be upregulated and secreted by –Pi Arabidopsis to scavenge Pi from extracellular organic-P. However, the novel AtPAP25 has never been suggested to be involved in the plant Pi-starvation response. Biochemical characterization of AtPAP25 revealed a monomeric 55 kDa protein. Similar to other PAPs it was purple-in-solution and insensitive to tartrate. Glycoprofiling via LC MS/MS revealed highly complex NXS/T glycosylation motifs at Asn172, Asn367 and Asn424. I hypothesize that these motifs play a role in AtPAP25 targeting and function. Kinetic characterization revealed a broad pH optimum centered at 5.6 and inhibition of activity by several common APase inhibitors. AtPAP25 exhibited broad substrate selectivity, low Vmax, and a Km (phosphoenolpyruvate) value of 0.52 mM. Immunoblot and semi-quantitative RT-PCR transcript analysis indicated that AtPAP25 is exclusively synthesized under –Pi conditions. Deduced amino acid sequences were compared using multiple sequence alignment and phylogenetic analysis. Growth of atpap25 T-DNA insertion mutant knockout seedlings was completely arrested when transferred to a soluble Pi deficient organic-P containing soil mix, pointing to a potential regulatory function of AtPAP25 during nutritional Pi stress. Overall, this research is helping to shed light on the functional importance of specific PAP isozymes in facilitating plant acclimation to nutritional Pi deficiency. This is important because there is an urgent need to engineer Pi-efficient transgenic crops to minimize the huge input of expensive, non-renewable, and polluting Pi fertilizers in agriculture. / Thesis (Master, Biology) -- Queen's University, 2012-09-10 08:28:21.631

The following research developed an approach and methodology to simultaneously gather and integrate social and natural science farm system data of developing countries into one data base. The overall approach was based on Weber's theory of abstraction, which requires the identification of the broadest number of variables as possible. The first step to understanding the farm system was to overview a number of the key variables which represented a number of key farm components; the second step was to juxtapose and blend together the various forms of data in linear forms against a test variable of Striga infestation levels; the third step was to evaluate if the amount of knowledge gained in predicting Striga infestation levels was statistically significant by cross correlating soil nutrient levels, crop management approaches, farmers' perceptions of Striga infestation and spatial distances; the fourth step was to use parametric and non-parameterc analytical tools in conjunction with data compression to locate the best combination of parameters to better manage Striga. The final part of the process was to identify and integrate the crop, field and social data into a profile of farmer's who have the highest and lowest likelihood of being infested by Striga by using a soil nutrient concentration baseline as the indicator. The results were that natural and social science data could be successfully combined, integrated and have statistically significant cross correlations. These correlations indicate that specific spatial parameters combined with specific soil components, farmer's management and crop placement could be used as predictors of Striga infestation levels. As well the farmers' perception could be validated using natural science data.

Feminism in the early 1980's in the United States revolved much around social and cultural matters such as sexual liberation, self- definition and self- realization for women. Derived from these ideas within feminism comes Alice Walker's Womanism, that is the writer's own definition of the strong and independent woman of color. This paper investigates the character Shug Avery, in The ColorPurple (1983), in relation to feminism and Womanism. It is argued that she is an empowered female because of the characteristics and attributes that come along with being a Womanist, despite moral,cultural and societal conditions that indicate marginalization for Shug and all women.

Decreases in ocean pH through ocean acidification has shown to have direct negative impacts on the early life stages of the Pacific purple sea urchin, Strongylocentrotus purpuratus. Research has suggested that multiple stressors could exacerbate, cancel, or even alleviate the impacts of ocean acidification on echinoderms. This study assessed the combined effects of changes in pCO2 concentrations (390, 800, 1500 ppm), salinities (28, 31, 34 ppt) and temperatures (12, 15, 18°C) on fertilization and larval development in S. purpuratus. Increased pCO2 was the predominant stressor, with additive and antagonistic effects from temperature changes, and no effect from salinity changes. Stressor combinations significantly decreased the rate of normal larval development by 28 – 68%, whereas fertilization and larval survival were unaffected. The strong impact on normal larval development likely indicates that later development stages could be detrimentally affected and could influence the population dynamics of S. purpuratus.

No description available.

A three-year (1991-1993) investigation was done in the field and laboratory to study the bionomics of Smicronyx guineanus Voss and Sm. umbrinus Hustache and the spatial distribution of Striga hermonthica (Del.) Benth. Studies on the life history of the weevils were undertaken. Seasonal activity and habits were established by monitoring life stages in the field. A behavioral Time Budget of Smicronyx adults was established in semi-field conditions. Dispersion patterns and the optimum sample size for Smicronyx and Striga were determined. Smicronyx adults as well as Striga plants were aggregated in the field. Smicronyx larvae were located in the upper stratum of the witchweed (93.2%) where they make galls and destroy large number of seeds. The effect of precipitation on emergence of Smicronyx adults was investigated in the insectary. The optimum rainfall required for Smicronyx adult emergence ranged from 30 to 40 mm. Good synchrony and positive association of Sm. guineanus and Sm. umbrinus with S. hermonthica were observed. There are good prospects for augmentation and/or conservation of Smicronyx populations as part of an integrated control strategy of S. hermonthica.

Thesis (M.S.)--Oregon State University, 2004. / Printout. Includes bibliographical references (leaves 84-90). Also available via the World Wide Web.