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EVALUATION OF SOYBEAN RECOMBINANT INBRED LINES FOR YIELD POTENTIAL AND RESISTANCE TO SUDDEN DEATH SYNDROMEAnderson, James Arthur 01 May 2012 (has links)
Evaluation of soybean recombinant inbred lines for seed weight yield, agronomic traits, and resistance to sudden death syndrome Sudden death syndrome (SDS) caused by Fusarium virguliforme is a devastating disease in soybean (Glycine max (L.) Merr.) that causes up to 70% of yield losses depending on the developmental stage when the plant become infected. The characterization of resistance is greatly significant for disease management. Therefore, three populations were developed by crossing three resistant lines, `Hamilton', LS90-1920 and LS97-1610 with a susceptible line to SDS, `Spencer'. Ninety-four F5:6 recombinant inbred lines from each population (Hamilton x Spencer, LS90-1920 x Spencer, and LS97-1610 x Spencer) were evaluated for two years (2009 and 2010) at two locations (Carbondale and Valmeyer) in southern Illinois. Population statistics, genotype x environment interaction, and broad-sense heritability were used to reveal any major resistance genes. Genetic correlation coefficients of SDS resistance with important agronomic traits such as lodging, pubescence, growth habit, and plant height were also calculated. The information from this study will be helpful to breeders in developing populations for genetic analyses and enforcing selection practices.
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Mission Analysis and Reporting System (MARS) - EW Analysis and Reporting On A Personal ComputerBurton, Ken 10 1900 (has links)
International Telemetering Conference Proceedings / October 28-31, 1996 / Town and Country Hotel and Convention Center, San Diego, California / In response to the need to analyze and report upon Electronic Warfare (EW) test
data results in a comprehensive and uniform manner, the Mission Analysis and
Reporting System (MARS) has been developed.
MARS is a government owned PC based Windows application designed for rapid
analysis and reporting upon EW test mission data. MARS currently performs
Jammer Effectiveness ( Reduction In Lethality, Increase In Survivability,
Reduction In Shot, and Reduction In Hit), Radar Warning Receiver (RWR)
System performance (Threat ID, Response Time/Ageout, and Direction Finding
(DF) Accuracy), and Tracking Error Statistics. Additionally, MARS produces
several graphical outputs including polar plotting, dynamic strip charting,
Cumulative Distribution Functions (CDF), and RWR Simulated Scope. Continual
development and maintenance of MARS at the Air Force Development Test
Center, Eglin Air Force Base, Florida, has provided a proven product used by
numerous DT&E and OT&E test projects over the last four years.
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Quantitative trait loci affecting the agronomic performance of a Sorghum bicolor (L.) Moench recombinant inbred restorer line populationMoran Maradiaga, Jorge Luis 30 September 2004 (has links)
Lately the rate of genetic gain in most agronomic crop species has been reduced due to several factors that limit breeding efficiency and genetic gain. New genetic tools and more powerful statistical analyses provide an alternative approach to enhance genetic improvements through the identification of molecular markers linked to genomic regions or QTLs controlling quantitative traits. The main objective of this research was to identify genomic regions associated with enhanced agronomic performance in lines per se and hybrid combination in Sorghum bicolor (L.) Moench. A population composed of 187 F5:6 recombinant inbred lines (RIL) was derived from the cross of restorer lines RTx430 and RTx7000. Also, a testcross hybrid population (TCH) was developed by using each RIL as a pollinator onto ATx2752. A linkage map was constructed using 174 marker loci generated from AFLP and SSR primer combinations. These markers were assigned to 12 different linkage groups. The linkage map covers 1573 cM with marker loci spaced at an averaged 9.04 cM. In this study, 89 QTL that control variation in seven different morphological traits were identified in the recombinant inbred line population, while in the testcross hybrid population, 79 QTL were identified. These traits included grain yield, plant height, days to mid-anthesis, panicle number, panicle length, panicle exsertion and panicle weight. These putative QTL explained from 4 to 42% of the phenotypic variation observed for each trait. Many of the QTL were not consistent across populations and across environments. Nevertheless, a few key QTL were identified and the source of the positive additive genetics isolated. RTx7000 was consistently associated with better agronomic performance in RIL, while in testcrosses, RTx430 was. Some genomic regions from RTx7000 may be utilized to improve RTx430 as a line per se. However, it is very unlikely that such regions will have a positive effect on the combining ability of RTx430 since testcross results did not reveal any transgressive segregants from the RIL population.
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Quantitative trait loci affecting the agronomic performance of a Sorghum bicolor (L.) Moench recombinant inbred restorer line populationMoran Maradiaga, Jorge Luis 30 September 2004 (has links)
Lately the rate of genetic gain in most agronomic crop species has been reduced due to several factors that limit breeding efficiency and genetic gain. New genetic tools and more powerful statistical analyses provide an alternative approach to enhance genetic improvements through the identification of molecular markers linked to genomic regions or QTLs controlling quantitative traits. The main objective of this research was to identify genomic regions associated with enhanced agronomic performance in lines per se and hybrid combination in Sorghum bicolor (L.) Moench. A population composed of 187 F5:6 recombinant inbred lines (RIL) was derived from the cross of restorer lines RTx430 and RTx7000. Also, a testcross hybrid population (TCH) was developed by using each RIL as a pollinator onto ATx2752. A linkage map was constructed using 174 marker loci generated from AFLP and SSR primer combinations. These markers were assigned to 12 different linkage groups. The linkage map covers 1573 cM with marker loci spaced at an averaged 9.04 cM. In this study, 89 QTL that control variation in seven different morphological traits were identified in the recombinant inbred line population, while in the testcross hybrid population, 79 QTL were identified. These traits included grain yield, plant height, days to mid-anthesis, panicle number, panicle length, panicle exsertion and panicle weight. These putative QTL explained from 4 to 42% of the phenotypic variation observed for each trait. Many of the QTL were not consistent across populations and across environments. Nevertheless, a few key QTL were identified and the source of the positive additive genetics isolated. RTx7000 was consistently associated with better agronomic performance in RIL, while in testcrosses, RTx430 was. Some genomic regions from RTx7000 may be utilized to improve RTx430 as a line per se. However, it is very unlikely that such regions will have a positive effect on the combining ability of RTx430 since testcross results did not reveal any transgressive segregants from the RIL population.
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Development of Genetic Linkage Maps and Identification of Quantitative Trait Loci Influencing Seed Oil Content, Fatty Acid Profile and Flowering Time in Brassica napus L.Javed, Nasir January 2014 (has links)
Identification of allelic variation through quantitative trait loci (QTL) mapping offers possibilities for the improvement of quantitatively inherited traits. This requires a genetic map along with the phenotypic characterization of a mapping population. A doubled haploid (DH) Polo X Topas population consisting of 194 lines and a recombinant inbred line population of 92 lines was developed. Individual genetic maps derived from each population were integrated into a consensus map. The DH-based genetic map was used for QTL mapping. The DH-based map was comprised of 620 loci that were assembled into 19 linkage groups that were anchored to the B. napus chromosomes. The DH-based map covered 2244.1 cM genomic distance with an average marker interval of 3.7 cM.
The DH population was phenotyped in four environments with each line replicated twice in a randomized complete block design. Days to flowering was recorded and oil content and fatty acid composition were determined using Near Infrared spectroscopy (NIR) and Gas Chromatography, respectively.
Fourteen QTL were identified for oil content, 33 QTL for palmitic acid content, 18 QTL for stearic acid content, 21 QTL for oleic acid content, 20 QTL for linoleic acid content, 23 QTL for linolenic acid content, 16 QTL for arachidic acid content and 14 QTL for flowering time.
Oil content QTL were identified on five linkage groups, A3, A10, C1, C5, and C6. An oil content QTL, qOIL-A10c appeared in all four environments, whereas qOIL-A10a appeared in only one environment but explained 26.99% variation. The oil content in the population ranged from 35% to 55.5% with the parents having values of 42% to 46%.
Two genomic regions on C3, with map positions at 147.83 cM and 154.55 cM harbored QTL (rQTL) for all the fatty acids studied. The additive effects of the rQTL reveal a correlation pattern which is supported by the phenotypic correlation observed between the fatty acids. This suggests rQTL have role in the fatty acid composition and possibly determine total seed oil content. The rQTL and flanking markers of the identified QTL offer utility in further development of B. napus. / October 2015
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Bacterial and Fungal composition of Sorghum bicolor: a metagenomics and transcriptomics analysis using next-generation sequencingMasenya, Kedibone 09 1900 (has links)
Sorghum crop has become attractive to breeders due to its drought tolerance, and many uses
including a human food source, animal feed, industrial fibre and bioenergy crop. Sorghum,
like any other plant, is a host to a variety of microbes that can have neutral, negative or positive
effects on the plant. While the majority of microorganisms are beneficial, pathogens colonize
plant tissues and overwhelm its defence mechanisms. This colonization is a direct threat to the
sorghum productivity. The development of microbiome-based approaches for sustainable crop
productivity and yield is hindered by a lack of understanding of the main biotic factors
affecting the crop microbiome. Metabarcoding has proven to be a valuable tool which has been
widely used for characterizing the microbial diversity and composition of different
environments and has been utilized in many research endeavours. This study analyses the
relationship between the microbiota and their response to natural pathogen infection in
sorghum disease groups (R, MR, S and HS) and identifies the most dominant pathogen in the
highly susceptible disease group. The study also, assesses the spore viability through the use
of the automated cell counter and confirms Fusarium graminearum (dominant pathogen linked
to the HS disease group) through sequencing of the marker genes, to subsequently characterize
pathways likely to be involved in pathogen infection resistance. To achieve the objectives, a
combination of 16S rRNA (V3/V4 regions) and ITS (ITS1/ITS4) of the internal transcribed
spacer regions were amplified and sequenced using NGS technologies to study the microbiota
in response to natural infection. Additionally, comparative transcriptional analysis of sorghum
RILs in response to Fusarium graminearum infection was conducted through RNA-Seq.
Upon natural infection, the foliar symptoms assessment of the RILs was conducted and four
disease groups; resistant (R), moderately resistant (MR), susceptible (S) and highly susceptible
(HS) were designated. The results of the present metabarcoding study indicate that resistant
sorghum leaves (R group) supported a large diversity of fungal and bacterial microbes. The
genera Methylorubrum, Enterobacter and Sphingomonas with reported plant growth
promoting traits were more abundant and highly enriched in the R and MR group, with
members of the latter genus significantly enriched in the R group. The resistant fungal group
had a majority of OTUs showing similarity to well-known plant growth-promoting fungal genus including Papiliotrema (Tremellaceae family), which are known biocontrol agents. The
yeast Hannaella was also highly linked with the resistant plants. Some Hannaella species are
known to produce indole acetic acid (IAA) for promoting plant growth.
Metabarcoding was also used to assess the major potential disease-causing taxa associated with
the highly diseased group. It identified fungal pathogenic species, that have not previously
been identified as pathogens of sorghum such as Ascochyta paspali and Ustilago kamerunensis
(which are known pathogenic fungi of grass species) and were associated with the susceptible
disease groups (S and HS). These analyses revealed the potential sorghum fungal pathogen
Epicoccum sorghinum, and was highly linked with the S disease group. It further expanded the
identification of a reportedly economically importance species causing sorghum related
diseases Fusarium graminearum (anamorph Gibberella zeae). This species has also been
identified in this study to be highly associated with the RILs showing major disease symptoms.
Fusarium graminearum a significant pathogen in winter cereals and maize has been associated
with stalk rot of sorghum and sorghum grain mould. The presence of Fusarium graminearum
in sorghum can be a toxicological risk, since this species has the potential to produce
mycotoxins. It was further shown that natural pathogen infection results in distinct foliar
microbial communities in sorghum RILs. The co-occurrence taxa represented by
Tremellomycetes and Dothiomycetes fungal classes and Bacillaceae and Sphingomonadaceae
bacterial family had more central roles in the network. The modules which are located centrally
on the network have been expected to play important ‘topological roles’ in interconnecting
pairs of other fungal and bacterial taxa in the symbiont–symbiont co-occurrence network.
These taxa having a central role, are considered to be keystone microbes, and have been
suggested to be drivers of microbiome structure and functioning. The results of bacterial and
fungal community composition, community co-occurrences further suggested the importance
of keystone taxa which may disproportionately shape the structure of foliar microbiomes. The
foliar disease symptom assessments revealed that sorghum RIL 131 was highly diseased and
RIL 103 did not show any visible disease symptoms and were subsequently used for
transcriptomic analysis. Gene expression patterns were studied between the identified RIL that did not show visible
symptoms (resistant RIL no 103) and the RIL that showed major disease symptoms
(susceptible RIL no 131). Fusarium graminearum the dominant potential pathogen found in
this study to be associated with the highly susceptible plants was used to inoculate RILs at
seedling stage in a greenhouse and samples were collected in triplicates at 24 hours post
infection (hpi), 48 hpi, 7 days post infection (dpi) and 14 dpi. Prior to that, ITS and UBC genes
confirmed the identity of Fusarium graminearum, and the automated haemocytometer
confirmed the cell/spore viability. Using RNA-Seq analysis it was shown that the resistant RIL
had defence related pathways from early response (24- 48 hpi) to late response (7-14 dpi). And
the more the infection progressed, the more the defence related genes were up-regulated in
terms of fragments per kilobase of exon model per million reads mapped (FPKM) and False
Discovery Rate (FDR ≤ 0.05) values.
Transcriptome time series expression profiling was used to characterize the plant response to
Fusarium graminearum with the Dirichlet Process Gaussian Process mixture model software
(DPGP) in susceptible and resistant RILs. The susceptible RIL (number 131) transcriptional
response upon Fusarium graminearum infection presented differences of the closely related
clustered expression profiles across all timepoints in both RILs. Group 2 exclusively clustered
the genes encoding the sesquiterpene metabolism pathway, which is one of the major
physiological change occurring in response to fungal infection and has been previously
reported to produce the mycotoxins associated with Fusarium head blight (FHB) of cereals.
This pathway presented an increase from the initial infection phase to the late infection phase
in group 4, the genes encoding starch sucrose, metabolism and cyanoamino acid pathways
presented a pattern that had a sharp decline from 48 hpi -14 dpi (at a later stage of infection).
This could suggest that, as the time progresses in the susceptible RIL the pathways which are
important in plant defence declines at a late infection stage. Group 3 presented a pattern
increase of the 5-lipoxygenase (LOX 5) gene expressed from 48 hpi-14 dpi timepoints. The
loss and silencing of LOX5 function have in the past described to be linked with enhanced
disease resistance. In this study the LOX5 was expressed and this could suggest that LOX5
might have a function as a susceptibility factor in disease caused by Fusarium graminearum
in sorghum RILs. CBL-interacting protein kinase 6 (CIPK6) gene was also associated with this group. This gene has been associated with negative regulation of immune response to
Pseudomonas syringae in Arabidopsis as plants overexpressing CIPK6 were more susceptible
to Pseudomonas syringae.
Transcriptional response of a resistant RIL (number 103) to infection with Fusarium
graminearum presented an increase in genes encoding metabolic and biosynthesis of
metabolites pathways in group 1 and group 4 at early infection phase and a sharp decline in
the late infection phase. An increase in the genes encoding pathways in earlier infection state
could suggest the establishment of a beneficial energy balance for defence. Additionally, genes
encoding phenylpropanoid (PAL), galactose and glycolysis pathway were amongst the genes
increased at early stages of infection in group 1. Sugar can play a significant role in resistance
to fungal pathogens through phenylpropanoid metabolism stimulation, and previous studies
showed that the phenylpropanoid pathway could play a role in resistance of wheat to Fusarium
graminearum and deoxynivalenol.
Overall, this study represents a first step in understanding the molecular mechanisms involved
in resistance to Fusarium graminearum. This analysis has also identified the reported
beneficial microbes and defence related genes and pathways. Together, the current findings
suggest that different ‘resident’ consortia found in naturally infected and uninfected sorghum
plants may be viable biocontrol and plant-growth promoting targets. Cultivation studies may
shed light on the nature of the putative symbiotic relationships between bacteria and fungi.
These results have consequences for crop breeding, and the analysis of microbial diversity and
community composition can be useful biomarkers for assessing disease status in plants. The
transcriptome and metabarcoding data generated will help guide further research to develop
novel strategies for management of disease in sorghum RILs through the integrative approach
considering both beneficial microbes and defence related genes. This provides the baseline
information and will positively impact in the development of Fusarium graminearum resistant
genotypes in future through the integration/incorporation of beneficial microorganisms
(bacteria and fungi) and resistant genes in breeding strategies. / Life and Consumer Sciences / D. Phil. (Life Sciences)
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Leveraging genomic mapping and QTL analysis to enhance drought tolerance of cultivated peanut (Arachis hypogaea L.)Kumar, Naveen 19 September 2022 (has links)
Peanut (Arachis hypogaea L.) is second major legume crop grown after soybean in the United States, and its productivity is often limited by drought stress. Drought negatively impacts the yield and quality of peanut. Drought stress in peanut causes an annual loss of approximately $520 million in the United States. Improving peanut yield under water deficit conditions is crucial for peanut growers to maintain their profitability in the market. To achieve this, it is essential to either breed or adopt already available drought tolerant cultivars that can produce higher yield under water deficit conditions. Therefore, the objectives of this research were to (1) evaluate five commercially available virginia and runner type peanut cultivars for pod yield stability using multilocation trials by studying G x E interaction across 13 environments including year, location, and irrigation regime. Linn and Binns, AMMI, Shukla, Wricke's, Finlay and Wilkinson stability models were used to determine pod yield stability. Bailey and Sullivan showed higher stability and adaptability across all stability indices whereas Wynne and TUFRunner presented high mean productivity with lesser stability across environments reflecting specific adaptation to just a few environments. Bailey and Sullivan are recommended for sustainable production across the growing region of Virginia and Carolinas. The second objective (2) was identification of drought tolerance related quantitative trait loci (QTL) and genetic markers to facilitate the development of drought tolerant cultivars. Three diverse recombinant inbred line (RIL) populations, derived from crossing lines N05006 x N04074FCT (Pop-1), line N05006 x Phillips, an old virginia-type cultivar (Pop-2), and lines N08086olJCT x PI 585005 (Pop-3) were phenotyped for the Normalized Difference Vegetation Index (NDVI), Canopy Temperature Depression (CTD), SPAD-meter relative chlorophyll content of the leaves (SPAD) and wilting for QTL mapping. Mapping identified 27 minor QTL on eight chromosomes for all physiological characteristics, i.e NDVI, CTD, SPAD and wilting, with logarithmic of odds values ranging from 2.5 to 38.5 and the phenotypic variance explained by these traits from 1.04 to 11.46 %. There were 4 loci on chromosome 2 associated with NDVI in Pop-1 and Pop-3, explaining 1.8 to 10.38% of the phenotypic variation. These genomic regions may be important resources in peanut breeding programs to improve drought tolerance. Further research is needed to increase the marker density in order to fine map the identified QTL and validate markers linked with these regions. / Doctor of Philosophy / Peanut is a multi million-dollar industry in the United States, but water limitations have a detrimental impact on yield, quality, and grower income. Drought along with aflatoxin contamination are two major challenges faced by U.S peanut industry. Annual losses to peanut caused by drought are around $520 million in the United States. Irrigation can alleviate water shortage in drought prone regions, but around 65% of peanut production in U.S is under rainfed condition, meaning that only rainfall can satisfy peanut crop water requirements. The most feasible and economical solution to peanut growers under these circumstances is to adopt drought tolerant varieties. In this research, our goal was to facilitate breeding drought tolerant cultivars through identification of molecular markers associated with drought tolerance and to identify already available drought tolerant peanut cultivars that could be a game changer for the producers. Therefore, the objectives of my research were to (1) evaluate in multiple environments five commercially available virginia and runner type peanut cultivars for pod yield stability and grade factors. In this study, we considered 13 environments, including 4 years, 4 locations, and 2 water regime. Statistical tools including Linn and Binns, AMMI, Shukla, Wricke's, and Finlay and Wilkinson were used to determine pod yield stability. These stability indices showed that Bailey and Sullivan are more stable and adaptable across different locations in terms of yield, whereas Wynne and TUFRunner presented high mean yield with lesser stability showing specific adaptation to only few environments. Based on stability analysis, Bailey and Sullivan are recommended for sustainable production across different growing region of Virginia and Carolinas. The second objective (2) was to identify drought tolerance related genomic regions using three mapping populations. Phenotyping and genotyping of three diverse recombinant inbred line (RIL) populations, derived from crossing lines N05006 x N04074FCT (Pop-1), lines N05006 x Phillips, an old virginia-type cultivar (Pop-2), and lines N08086olJCT x PI 585005 (Pop-3) were done to find quantitative trait loci (QTL) for drought related traits. These population were phenotyped for the Normalized Difference Vegetation Index (NDVI), Canopy Temperature Depression (CTD), SPAD-meter relative chlorophyll content of the leaves (SPAD) and wilting for QTL mapping. These surrogate traits are related to trait of interest for drought tolerance. NDVI is effective in predicting biomass and yield. Similarly, CTD is associated with transpiration efficiency and carbon dioxide assimilation. Mapping identified 27 minor QTL on eight chromosomes for all physiological characteristics, i.e NDVI, CTD, SPAD and wilting with logarithmic of odds values range from 2.5 to 38.5 and the phenotypic variance explained by these traits ranging from 1.04 to 11.46 %. There were 4 loci on chromosome 2 associated with NDVI in Pop-1 and Pop-3, explaining 1.8 to 10.38% of the phenotypic variation. These genomic regions may be important resources in peanut breeding programs to improve drought tolerance. Further research is needed to increase the marker density in order to fine map the identified QTL and validate markers linked with these regions.
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Modelling socio-economic effects of implementing reduced-impact logging : a case study of Berau District East Kalimantan Province Indonesia.Martana, Kadim January 2013 (has links)
Reduced-impact logging (RIL) was identified as a measure, complementary to other identified measures, which can contribute to Indonesia reducing emissions from forestry and forest degradation (REDD+). In this light, the research was aimed to investigate the economy-wide impact of implementing RIL on the economy, which was studied at the district level. For this purpose, an expert opinion survey method was integrated with Berau computable general equilibrium (CGE) model. The expert opinion survey was utilised to: (i) generate information
and confirm the impact of implementing RIL on logging costs, and (ii) obtain an estimate of the incentive required by logging companies to maintain the practice of RIL. Results of this approach provided input to the Berau CGE model. In addition, multiple ways of data gathering were employed to develop the dataset required for the general equilibrium analysis.
Simulation results suggest that the economic impact of implementing RIL policy is negative but small on the Berau economy compared to a situation where the RIL is not implemented. Worker households (particularly agricultural worker households) are worse off and non-agricultural households are better off. Furthermore, providing compensation, which was simulated as a logging output- based subsidy, can improve the Berau economy, although to only a lesser degree.
The RIL policy causes a significant negative impact on logging output which further leads to output reduction in forest-based and pulp & paper industries. The policy implementation, however, simulates production increase in other agricultural activities, notably in oil palm plantation. Furthermore, results simulations with the logging output-based subsidy suggest the magnitude of economic impacts is reduced from what would otherwise occur in the scenario of implementing RIL only (no subsidy is provided).
The RIL policy also seems to result in ̳unexpected‘ emissions leakage indicated by increases in output of some agricultural-based activities such as oil palm plantation, other estate crops, and food crops. Increase in emissions is also expected to occur outside the Berau District stimulated by the increase in the District‘s import of timber.
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Genetic Basis of Tocopherol Accumulation in Soybean (Glycine Max [L.] Merr.) SeedsShaw, Eric 23 November 2012 (has links)
This thesis is an investigation of the genetic basis of tocopherol accumulation in soybean (Glycine max [L.] Merrill) seeds. Soybean is the world’s most widely grown protein and oilseed crop and the principle source of vitamin E (tocopherols) as a supplement. Tocopherols (α-, β-, γ- and δ-isomers) are powerful antioxidants that contain human health benefits, including a decrease in the risk of lung cancer, heart disease and osteoporosis. The purpose of this research was to identify genetic and biochemical components affecting tocopherol accumulation in soybean seeds. The objectives were to: 1) investigate location and year effects on soybean seed tocopherol levels in the field; 2) determine environmental factors affecting soybean seed tocopherol levels under controlled conditions; 3) identify simple sequence repeat (SSR) markers that tag quantitative trail loci (QTL) for individual and total tocopherols; and 4) evaluate the potential role of VTE1, VTE3 and VTE4 genes in tocopherol accumulation using the candidate gene approach. Seventy nine recombinant inbred lines (RILs) derived from the cross between OAC Bayfield and OAC Shire were grown in the field at Elora, Woodstock and St. Pauls, ON, in 2009 and 2010. The tocopherol components were quantified using high performance liquid chromatography (HPLC). The results showed a significant (p < 0.001) genotype, environment and genotype x environment effect for each tocopherol component. It was discovered that a 2 x phosphate fertilizer (K2SO4 at 1.0M/150mL) and 30 ˚C temperature treatment increased each tocopherol component, whereas drought had no effects. Single marker analysis identified 42 QTL and interval mapping identified 26 QTL across 17 chromosomes. Significant two-locus epistatic interactions were found with a total of 122 and 152 in the 2009 and 2010 field seasons, respectively. The multiple locus models explained 18.4% to 72.2% with an average of 45.7% of the total phenotypic variation. The candidate gene approach using nucleotide sequences from the coding regions identified two single nucleotide polymorphisms (SNPs) in VTE1, five SNPs in VTE4 and none in VTE3. The SNPs were predicted to cause functional protein changes and the genes co-localized with some of the identified QTL. The results of this study provide a better understanding of the environmental factors and genetic mechanisms that influence the accumulation of tocopherols in soybean seeds. / Grain Farmers of Ontario, Vitamin Research Award
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Islam et Chiisme politique / le cas du LibanWehbe, Rabih 07 March 2018 (has links)
Dans un essai qui aurait tout aussi bien pu s'intituler « L'islam chiite entre la politique et la religion, le cas du Liban », une analyse de l'islam politique demeure nécessaire pour mettre la lumière sur la différence entre islamisme et religion musulmane. Avec un peu moins de deux milliards de musulmans dans le monde, la religion musulmane est devenue la première religion pratiquée dans le monde actuel. Le dynamique de cette religion a permis la création d'un immense empire aux populations hétérogènes. Ainsi que, la coexistence entre religion et régime politique a provoquée de véritables luttes armées entre les grandes familles politico-religieuses, notamment le sunnisme et le chiisme. Le sunnisme ayant souvent l'étiquette d'orthodoxie a gardé cette aspect alors que le chiisme devint autres chose ce qu'il était à l'origine, lorsqu'on y voyait seulement le parti qui s'était rassemblé autour d'Ali ibn Abî Tâlib, cousin et gendre du prophète Mohammad. Dans ses quelques traitements de la doctrine islamique nous constatons que ces familles politico-religieuses, tant sunnites que chiites, proliférèrent les unes à côté des autres en même temps qu'elles se combattirent et souvent se condamnèrent réciproquement. Ceci est dû au fait qu'en l'islam, il n'y a jamais eu de pouvoir interprétatif qualifié, individuel ou collégial capable de s'imposer sans conteste.L'effondrement de l'Empire Ottoman donna l'occasion à la France et la Grande-Bretagne de partager le monde arabe sur la base des fameux accords Sykes-picot. La France va restructurer les territoires syrien et libanais, elle établit la structure constitutionnelle confessionnelle complexe du Liban, faisant du pays de Cèdre le plus grand laboratoire du communautarisme. Dans le Liban d'après-guerre, le communautarisme va de soi, il reflète l'état de la société et celle de la conciliation entre spécificités confessionnelle et principe fondamental de l'Etat nation. Le communautarisme libanais va évoluer à travers des mutations économiques, sociales et politiques, notamment chez la communauté chiite. Nous présenterons l'évolution de la communauté chiite dans cet environnement, ainsi que le rôle fondamental joué par Moussa el-Sadr dans la libération de la communauté chiite. Son objectif étant une réaction à la conscience politique du «Metwali». Sa première action était la lutte contre les inégalités sociales et devait s'engager avec l'Etat libanais dans une série d'affrontements qui couvraient souvent un aspect spectaculaire: grève générale en 1970, avertissement au gouvernement et à la réunion de 1974 à Baalbeck que Moussa-El -Sadr a annoncé la naissance du mouvement AMAL. Ce mouvement joue un rôle essentiel dans la vie politique libanaise. Enfin, nous consacrons une partie de ce travail à l'émergence d'une milice radicale pro-iranienne chiite, le Hezbollah, qui a pénétré le système politique libanais. Sa place est privilégiée à cause de ses succès dans la résistance contre Israël, de ses actions sociales et humaines et de ses organisations. La timide participation du « parti de Dieu » au parlement libanais constituait un premier pas vers la « libanisation » du parti. En 2012, le parti chiite annonce sa participation aux combats en Syrie à côté de l'armée de Bachar el-Assad, freinant ainsi le processus de libanisation. Le Hezbollah devient un des acteurs incontournables de la géopolitique du Moyen Orient et retourne en force sur la scène politique libanaise pour s'inscrire dans le cadre d'un chiisme politique international. / In an essay that might as well have been entitled "Shia Islam between politics and religion, the case of Lebanon", an analysis of political Islam remains necessary to shed light on the difference between Islamism and religion Muslim. With just under two billion Muslims in the world, the Muslim religion has become the first religion practiced in the world today.The dynamics of this religion allowed the creation of an immense empire with heterogeneous populations. As well as, the coexistence between religion and political regime provoked real armed struggles between the big politico-religious families, notably Sunnism and Shiism.Sunnism often had the label of orthodoxy, but Shiism became something else that it was originally when one saw only the party that had gathered around Ali ibn Abi. Tâlib, cousin and son-in-law of the prophet Mohammad. In his few treatments of Islamic doctrine we find that these politico-religious families, both Sunni and Shiite, proliferated alongside each other at the same time that they fought each other and often condemned each other. This is due to the fact that in Islam there has never been a qualified, individual or collegiate interpretative power capable of imposing itself unquestionably.The collapse of the Ottoman Empire gave France and Britain the opportunity to share the Arab world on the basis of the famous Sykes-picot agreements. France will restructure the Syrian and Lebanese territories, it establishes the complex confessional constitutional structure of Lebanon, making the country of Cedar the largest laboratory of communitarianism. In post-war Lebanon, communitarianism is self-evident, reflecting the state of society and the reconciliation of confessional specificities with the fundamental principle of the nation-state. Lebanese communitarianism will evolve through economic, social and political changes, especially among the Shia community.We will present the evolution of the Shiite community in this environment, as well as the fundamental role played by Moussa el-Sadr in the liberation of the Shia community. His goal is a reaction to the political conscience of "Metwali". Its first action was the fight against social inequalities and was to engage with the Lebanese State in a series of clashes that often covered a spectacular aspect: general strike in 1970, warning to the government and to the 1974 meeting in Baalbeck that Moussa-El -Sadr announced the birth of the AMAL movement. This movement plays a vital role in Lebanese politics. Finally, we devote part of this work to the emergence of a radical pro-Iranian Shiite militia, Hezbollah, which has penetrated the Lebanese political system. His place is privileged because of his successes in the resistance against Israel, his social and human actions and his organizations. The timid participation of the "party of God" in the Lebanese parliament was a first step towards the "libanization" of the party. In 2012, the Shiite party announced its participation in the fighting in Syria next to the army of Bashar al-Assad, thus curbing the process of Lebanization. Hezbollah becomes one of the key players in the geopolitics of the Middle East and returns in force on the Lebanese political scene to be part of an international political Shiism.
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