Despite drought accounting for over 80% of agricultural losses, little progress has been made towards improving drought tolerance in crops. My approach to identifying traits underlying drought tolerance involved a comparison between two accessions of the crucifer, Eutrema salsugineum, that display differential tolerance to water deficits. The accessions, originating from the semi-arid Yukon, Canada, and a monsoonal region of Shandong, China, were subjected to a two-step, water deficit and recovery protocol to identify physiological characteristics that discern their drought-responsive behaviour. Traits that discriminate between the ecotypes were used to screen recombinant inbred lines (RILs) that were generated by crossing Yukon and Shandong parent plants. Selected physiological measurements were: anthocyanin accumulation, cut rosette water loss (CRWL), solute potential, relative water content (RWC), static leaf water content (SLWC), specific leaf area (SLA), and OJIP fluorescence emission. Of the measurements taken, CRWL measurements and anthocyanin content distinguished the Yukon ecotype from the Shandong ecotype during the first drought exposure whereas SLA and fluorescence responses differentiated these accessions better after plants that experienced the first drought were rewatered and recovering or undergoing a second drought treatment. Sixty-eight RILs were screened using SLA and OJIP fluorescence emission. SLA and OJIP measurements varied among the recombinant inbred lines (RILs) with many lines showing responses to water deficit intermediate to those of the parental lines. Evidence of heritability in SLA and/or OJIP responses to water deficits would make them useful phenotypic markers for identifying quantitative trait loci (QTLs) associated with drought tolerance in future work. / Thesis / Master of Science (MSc)

Master of Science / Department of Agronomy / Allan K. Fritz / Fusarium head blight (FHB), caused by Fusarium graminearum, is an important cereal disease in humid and semi-humid wheat growing regions. In recent FHB epidemics in the USA, FHB dramatically reduced wheat yields and grain quality due to mycotoxin contamination. Five types of FHB resistance have been reported, but resistance to disease spread within a spike (Type II) and low deoxynivalenol (DON) accumulation in infected kernels (Type III) have drawn the most attention. A Chinese Spring-Sumai3 chromosome 7A substitution line (CS-SM3-7ADSL) was reported to have a high level of Type II resistance, but quantitative trait locus (QTL) on chromosome 7A has never been mapped. To characterize QTL on chromosome 7A, we developed 191 Chinese Spring-Sumai3-7A chromosome recombinant inbred lines (CRIL) from a cross between Chinese Spring and CS-SM3-7ADSL and evaluated the CRIL in a greenhouse for both types of resistance in three experiments. Two major QTL with Sumai 3 (SM3) origin, conditioning Type II and Type III resistance were mapped in chromosomes 3BS and 7AC. QTL on chromosome 3BS corresponds to Fhb1, previously reported from SM3, whereas 7AC QTL, designated as Fhb5, is a novel QTL identified from SM3 in this study. Fhb5 explains 22% phenotypic variation for Type II resistance and 24% for Type III resistance. Marker Xwmc17 is the closest marker to Fhb5 for both types of resistance. Fhb1 and Fhb5 were additive and together explained 56% variation for Type II and 41% for Type III resistance and resulted in 66% reduction in FHB severity and 84% in DON content. Both QTL showed significant pleiotropy effects on Type II and Type III resistance, suggesting both types of resistance may be controlled by the same gene(s). Haplotype analysis of SM3’s parents revealed that Fhb5 originated from Funo, an Italian cultivar. A survey of worldwide germplasm collection of 400 accessions showed that Fhb5 is present mainly in Chinese cultivars, especially in Funo-related accessions. Further, Fhb5 is the second major QTL from SM3 and have potential to be used in improving wheat cultivars for both types of resistance.

The majority of genetic differences between species and individuals have been hypothesised to impact on the regulation, rather than the structure, of genes. As the details of genetic variation are uncovered by the various genome sequencing projects, understanding the functional effects on gene regulation will be key to uncovering the molecular mechanisms underying the genesis and inheritance of common phenotypes, such as complex human disease and commercially important traits in plants and animals. Unlike coding sequence polymorphisms, genetic variants affecting gene expression will reside in the transcriptional machinery and its regulatory inputs. As these are largely specific to cell- or tissue-types, we would expect that regulatory variants will also affect final mRNA levels in a tissue specific manner. Genetic variation between individuals may therefore be more complex than the sum total of sequence differences between them. Demonstrating this hypothesis is the main focus of this thesis. We use microarrays to measure mRNA levels of approximately 22,000 transcripts in inbred and recombinant inbred strains of mice, and present compelling evidence that the genetic influences on these levels are tissue-specific in at least 85% of cases. We uncover two loci which apparently influence transcript levels of multiple genes in a tissue-specific manner. We also present evidence that failure of microarray data normalisation may cause spurious linkage of expression phenotypes leading to erroneous biological conclusions, and detail a novel, extensible mathematical framework for performing tailored normalisation which can remove such systematic bias. The wider context of these results is then discussed.

Stem rust and Yellow rust diseases are the two major wheat fungal diseases causing considerable yield losses in Turkey and all around the world. There are studies which are carried out to identify and utilize resistance sources in order to obtain resistant lines of wheat. However, virulent pathotypes are continuously being important threats to wheat production and yield. For that reason, new approaches for rapid identification are needed. The aim of this study was to investigate and to understand the structural and functional differences between the resistant and sensitive durum wheat cultivars to the plant fungal diseases of stem and yellow (stripe) rusts. To aim this, forty durum wheat recombinant inbred lines (RILs), which were previously determined to be resistant or sensitive to stem and yellow rust diseases, were investigated by the noninvasive Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy. Also, classification of the resistant and sensitive lines depending on the structural and functional differences has been attempted. The FTIR spectra for stem rust disease showed that, resistant durum wheat lines had a significant increase in the population of unsaturation in acyl chains of lipid molecules, an increase in lipid and in total protein content and also an increase in carboxylic acids and alcohols. For yellow rust disease, resistant lines had a significant increase in hydrogen bonding and they had also a more ordered membrane structure. In Principal Component Analysis for stem rust disease, according to 3700-650 cm-1 region, amide III band (1213-1273 cm-1 region) and C-H stretching region (3020- 2800 cm-1), the resistant and sensitive groups were separated successfully. For yellow rust disease, according to 3700-650 cm-1 region, Amide A and Amide III bands, the resistant and sensitive lines were grouped distinctly. FTIR spectroscopy provides a useful approach to determine the differences in molecular structure of durum wheat RILs regarding resistance of lines to fungal diseases. However, further research is still needed to ensure if the structural and functional differences in biomolecules of the samples could be used as molecular markers for discrimination of rust resistant materials from rust sensitive ones.

Orientadores: Luciana Lasry Benchimol Reis, Matthew Ward Blair / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-23T08:28:45Z (GMT). No. of bitstreams: 1 BrinezRodriguez_Boris_D.pdf: 6826443 bytes, checksum: 0422d2a5e7a57388d0cc9defcf04a3f2 (MD5) Previous issue date: 2013 / Resumo: O feijão comum (Phaseolus vulgaris L.) é uma cultura importante economicamente tanto para o consumo nacional como para a exportação. A seca é um dos principais estresses abióticos em todo o mundo e afeta cerca de 60% da área de cultivo de feijão. O avanço nas tecnologias de marcadores moleculares oferecem poderosos métodos para examinar as relações entre as características, gerando um grande volume de informações potencialmente úteis para assessorar os programas de melhoramento. O presente projeto teve como objetivo o desenvolvimento da Plataforma DArT para feijão comum junto à empresa DArT Pty Ltd, e o mapeamento destes marcadores juntamente com microssatélites e SNPs na população AND 277 x SEA 5 proveniente do CIAT (Colômbia), a fim de localizar os QTLs associados à tolerância à seca. O genitor SEA 5 é uma linhagem avançada do BAT 477, é tolerante à seca e de origem Mesoamericano e o genitor AND 277 é um genótipo resistente à mancha angular e antracnose e de origem Andina. Um total de 4.468 marcadores DArTs, 288 marcadores SNPs e 180 marcadores microssatélites polimórficos foram identificados na população e utilizados na genotipagem para construir um mapa genético saturado. A fenotipagem das 105 linhagens endogâmicas recombinantes (RILs) na geração F8 mais os dois genitores foi realizada avaliando 18 características associadas à tolerância a seca utilizando um delineamento inteiramente casualizado com quatro repetições, aplicando um estresse terminal na fase vegetativa V3/V4. Dois mapas foram construídos, um integrando 80 SSR e 251 SNPs e outro com cinco SSR, 91 SNPs e 4.468 DArTs. A identificação dos QTLs foi realizada através da análise de mapeamento por intervalo composto (CIM) para o mapa SSR - SNPs e mapeamento de precisão (SML) para o mapa SSR-SNPs-DArT. Um total de 12 QTLs foram identificados para o tratamento não irrigado e 29 QTLs para o tratamento irrigado pela análise CIM. Para as análises SML, 23 QTLs foram identificados para o tratamento não irrigado e 11 QTLs para o irrigado. QTLs de maior efeito foram encontrados para clorofila, biomassa fresca do caule e da folha, Massa seco da folia, temperatura da folha, número de vagens, número de sementes, massa de sementes, dias para florescimento, massa seca das vagens e produtividade nos dois tratamentos. Todos os QTLs detectados sob condições de seca apresentaram o alelo do genitor SEA 5. Este estudo é importante para o melhoramento genético não só para entender melhor a herança genética de uma característica tão complexa como a tolerância à seca, bem como para encontrar ferramentas moleculares a serem utilizados para a seleção assistida por marcadores / Abstract: Common bean (Phaseolus vulgaris L.) is the most important food legume for consumption and for exportation. Drought is one of the main abiotic stresses in the world and affects about 60% of bean growing area across the world. The advance in technologies of molecular markers provide a powerful method to examine the relationships between traits, generating large amount of potentially useful information to assist the breeding programs. The objective of this project was the development of DArT platform for common beans with DArT Pty Ltd and the mapping of these markers with microsatellites and SNPs in the population AND 277 x SEA 5 from CIAT (Colombia), in order to locate the QTLs associated with drought tolerance. The SEA 5 parent is a drought tolerant advanced line (Mesoamerican) and the AND 277 is resistant to the angular leaf spot and antracnose (Andean). A total of 4.468 DArT markers, 288 SNP and 180 SSR polymorphic markers were identified in the population and used in genotyping to constructed a saturated genetic map. Phenotyping of 105 recombinant inbred lines (RILs) in F8 generation plus the genitors were performed evaluating 18 traits associated with drought tolerance using a completely randomized design with four replicates, applying terminal stress at vegetative phase V3/V4. Two maps were constructed, one integrating 80 SSR and 251 SNPs and another with five SSR, 91 SNPs and 4,468 DArTs. The identification of QTL analysis was performed by composite interval mapping (CIM) for the SSR - SNPs map and the precision mapping (SML) to map DArT-SSR-SNPs. A total of 12 QTLs were identified for the non-irrigated treatment and 29 QTLs for the irrigated treatment by CIM analysis. For SML analysis, 23 QTLs were identified for the non-irrigated and 11 QTLs for irrigated treatment. QTLs of major effect was found for chlorophyll, fresh biomass of stem and leaf dry weight, leaf temperature, number of pods, number of seeds, seed weight, days to flowering, dry weight of pods and yield in both treatments. All QTLs detected under dry conditions showed the allele of parent SEA 5. This study is important for genetic improvement not only to better understand the genetic inheritance of a trait as complex as drought tolerance, as well as to find molecular tools to be used for marker assisted selection / Doutorado / Genetica Vegetal e Melhoramento / Doutor em Genetica e Biologia Molecular

Submitted by Onia Arantes Albuquerque (onia.ufg@gmail.com) on 2018-10-08T13:49:50Z No. of bitstreams: 2 Tese - Stella Cristina Dias Valdo - 2017.pdf: 4757114 bytes, checksum: 62d9148c9c6315952ac5a86bdb1f9417 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Rejected by Luciana Ferreira (lucgeral@gmail.com), reason: Olhe o espaço a mais na citação: VALDO, S. C. D. Estudo de resistência à murcha-de-fusarium e identificação de QTLs em feijeiro-comum. 2017. 178 f. Tese (ESPAÇO A MAIS Doutorado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2017. on 2018-10-09T10:59:43Z (GMT) / Submitted by Onia Arantes Albuquerque (onia.ufg@gmail.com) on 2018-10-09T11:17:13Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese - Stella Cristina Dias Valdo - 2017.pdf: 4757114 bytes, checksum: 62d9148c9c6315952ac5a86bdb1f9417 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-10-09T11:41:12Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese - Stella Cristina Dias Valdo - 2017.pdf: 4757114 bytes, checksum: 62d9148c9c6315952ac5a86bdb1f9417 (MD5) / Made available in DSpace on 2018-10-09T11:41:12Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese - Stella Cristina Dias Valdo - 2017.pdf: 4757114 bytes, checksum: 62d9148c9c6315952ac5a86bdb1f9417 (MD5) Previous issue date: 2017-04-27 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / The common bean (Phaseolus vulgaris) crop plays an important role in the culture and economy of Brazil. It is cultivated in all Brazilian regions and is affected by several diseases like fusarium wilt which is caused by Fusarium oxysporum f. sp. phaseoli (soil-born fungus). This disease brings significant losses in common bean culture and genetic resistance is the primary form of control. One of the core goals of breeding programs is the development of resistant cultivars, therefore the objectives of this work are: i) To select F. oxysporum f. sp. phaseoli resistant F5:7 lines resulted from the crossing between Ouro Branco X CNFP10132, under controlled field and environment conditions ii) To identify SSR markers and QTL-linked SNPs associated with the resistance of common bean to fusarium wilt using 92 recombinat inbred lines(RILs) resulted from the crossing between Ouro Branco x CNFP10132. In the first study, 140 lines, the breeders Ouro Branco and CNFP10132, BRS Esplendor (resistant) and BRS Supremo (susceptible) as controls were evaluated. Field trials were conducted in a center pivot area where natural infestation of the pathogen occurs. The treatments were evaluated in summer and winter crop and the experimental design used was 12x12 triple lattice. The two controlled environment trials were conducted in a completely randomized design. The treatments were inoculated by cutting and immersing the roots in a conidial suspension, which was adjusted to 1x106 conidia/ml for five minutes. The evaluation was performed using a scale of nine grades that represent the severity of the disease: 1 – absence of symptoms and 9 – over 75% of foliage with wilt symptoms. Data were submitted to analysis of variance and Scott-Knott test for both environments. The area under the disease progress curve (AUDPC) and genetic parameters were estimated for controlled environment tests. Significant differences were observed for crops and for controlled environment trials, indicating that environment influences directly the severity of the disease. Highly significant differences were found for lines in all environments evaluated, demonstrating the existence of genetic variability, which allows the selection of resistant lines resistant to fusarium wilt. Treatments were classified in different groups according to the Scott-knott test. When considering the lowest averages in field, controlled environment and AUDPC, the strains Ouro Branco x CNFP 10132.140, Ouro Branco x CNFP 10132.49, Ouro Branco x CNFP 10132.12, Ouro Branco x CNFP 10132.90 and Ouro Branco x CNFP 10132.48 were prominent and are candidates to produce a breeding program. Heritability estimates were high for all environments, mean of 85.48% for field and 95.47% for controlled environment. Therefore, selection for resistance to F. oxysporum f. sp. phaseoli of these lines, will be successful. In the second study it was extracted DNA from 92 lines and from genitors for genotyping with SSRs and SNPs. In order to obtain the localization of these markers, sequences of the primers were aligned to the andean genome of the common bean. The method of single marker (analysis of QTLs based on linear regression) was used to identify QTLs associated with fusarium wilt resistance. These markers were considered significant when brought up p-value <0.05. Ninety-three markers were linked to 104 QTLs associated with fusarium wilt resistance and among these, were considered significant in more than one environment PV 115, PV 251, BARC-PV-0004089, BARC-PV-0004548, BARC-PV-0003450, BARC-PV-0006051, BARC-PV-0003368 , BARC-PV-0005477 and BARC-PV-0004897. However only the BARC-PV-0003450 marker was highly significant in the two environment controled trials (p <0.001) and winter crop (p <0.01) and explained up to 21.5% of the phenotypic variance. Subsequently, the gene annotation was made considering the location of all markers that were significant at p <0.01 comprising 500 kb before and after the localization. 960 coded transcripts were annotated. It was observed in gene annotation that BARC-PV-0003450 marker is located on the chromosome 8, 338.54 kb distant of the gene Phvul.008G014700 which is associated with the putative protein RPP13 related to disease resistance, identified in Arabidopsis thaliana. This protein belongs to the third class of resistance genes that encloses the domain called Leucine-Rich Repeats (LRR). This domain is involved in the recognition of the pathogen by the host during the infection process. Therefore, this marker is suitable for marker- assisted selection aiming the development of cultivars resistant to fusarium wilt. / A cultura do feijoeiro-comum (Phaseolus vulgaris) tem importância cultural e econômica no Brasil. O feijoeiro-comum é cultivado em todas as regiões brasileiras e é acometido por várias doenças, como a murcha-de-fusarium, causada pelo fungo habitante de solo Fusarium oxysporum f. sp. phaseoli. Esta doença causa significativas perdas na cultura e a principal forma de controle é a resistência genética. Desenvolver cultivares resistentes é um dos alvos dos programas de melhoramento, portanto os objetivos deste trabalho foram: i) selecionar linhagens resistentes obtidas de população F5:7 oriunda do cruzamento entre Ouro Branco e CNFP10132 para F. oxysporum f. sp. phaseoli, em condições de campo e de ambiente controlado e ii) identificar marcadores SSR e SNP's ligados a QTLs associados à resistência do feijoeiro-comum à murcha-de-fusarium utilizando 92 linhagens recombinantes endogâmicas (RILs) derivadas do cruzamento Ouro Branco x CNFP10132. No primeiro estudo 140 linhagens, os genitores Ouro Branco e CNFP10132, duas testemunhas BRS Esplendor (resistente) e BRS Supremo (suscetível) foram avaliados. Os ensaios de campo foram conduzidos em área de pivô central onde ocorre infestação natural do patógeno. Os tratamentos foram avaliados em duas safras (safra das águas e de inverno) em delineamento de látice triplo 12x12. Os dois ensaios em ambiente controlado foram conduzidos em delineamento inteiramente causalizado. As plantas foram inoculadas utilizando o método de corte de raiz e imersão destas na suspensão de conídios, que foi ajustada para 1x106 conídeos/mL durante cinco minutos. A avaliação foi feita utilizando uma escala de notas de nove graus que representam a severidade da doença: sendo 1 - ausência de sintomas e 9 - acima 75% da folhagem com sintomas de murcha. Os dados foram submetidos à análise de variância e teste de Scott-Knott para os ambos ambientes. Para os ensaios em ambiente controlado foram estimados área abaixo da curva do progresso da doença (AACPD) e parâmetros genéticos. Foram observadas diferenças significativas para safras e para ensaios de ambiente controlado, indicativo de que o ambiente influencia diretamente na severidade da doença. Foram encontradas diferenças altamente significativas para linhagens em todos os ambientes avaliados, evidenciando a existência de variabilidade genética, o que possibilita seleção de linhagens resistentes à murcha-de-fusarium. Ao considerar as menores médias em campo, ambiente controlado e ACCPD as linhagens Ouro Branco x CNFP 10132.140, Ouro Branco x CNFP 10132.49, Ouro Branco x CNFP 10132.12, Ouro Branco x CNFP 10132.90 e Ouro Branco x CNFP 10132.48 se destacaram e são candidatas para compor o programa de melhoramento. As estimativas de herdabilidade foram altas para todos os ambientes, média de 85,48% para campo e 95,47% para ambiente controlado. Portanto, a seleção para resistência à F. oxysporum f. sp. phaseoli dentre estas linhagens, será bem sucedida. No segundo estudo foi extraído o DNA de 92 linhagens e dos genitores para genotipagem com marcadores SSRs e SNPs. Para obtenção da localização destes marcadores as sequências dos primers foram alinhadas no genoma andino do feijoeiro-comum. O método de mapeamento por marcas simples (análise de QTLs por meio da regressão linear) foi utilizado para identificar QTLs associados à resistência à murcha-de-fusarium. Foram considerados marcadores significativos os que apresentaram p-valor<0,05. Noventa e três marcadores foram identificados ligados a 104 QTLs associados à resistência à murcha-de-fusarium. Dentre estes marcadores destaca-se os que foram significativos em mais de um ambiente PV 115, PV 251, BARC-PV-0004089, BARC-PV-0004548, BARC-PV-0003450, BARC-PV-0006051, BARC-PV-0003368, BARC-PV-0005477 e BARC-PV-0004897. Dentre os marcadores, somente o marcador BARC-PV-0003450 foi altamente significativo nos dois ensaios, em ambiente controlado (p<0,001) e na safra de inverno (p<0,01), e explicou até 21,5% da variância fenotípica. Foi feita a anotação gênica considerando a localização de todos os marcadores que foram significativos à p<0,01 e abrangeu 500 kb anterior e posterior à localização. Foram anotados 960 transcritos codificados. Ainda observou-se que o marcador BARC-PV-0003450 está localizado no cromossomo 8 distante 338,54 kb do gene Phvul.008G014700 o qual está associado à proteína putativa RPP13 relacionada com resistência à doenças, identificada em Arabidopsis thaliana. Esta proteína pertence à terceira classe de genes de resistência que engloba o domínio denominado de Repetições Ricas em Leucina (LRR; Leucine Rich Repeats). Este domínio está envolvido no reconhecimento do patógeno pelo hospedeiro durante o processo de infecção. Portanto há a possibilidade de selecionar linhagens resistentes à murcha-de-fusarium e identificar QTLs que possivelmente estão ligados aos marcadores utilizados

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2017-05-15T13:58:20Z No. of bitstreams: 2 Dissertação - Ana Laura Pereira Passos - 2016.pdf: 6140952 bytes, checksum: 715de51ee1d8b3b7ffd295f5d7121216 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-05-15T13:58:44Z (GMT) No. of bitstreams: 2 Dissertação - Ana Laura Pereira Passos - 2016.pdf: 6140952 bytes, checksum: 715de51ee1d8b3b7ffd295f5d7121216 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-05-15T13:58:44Z (GMT). No. of bitstreams: 2 Dissertação - Ana Laura Pereira Passos - 2016.pdf: 6140952 bytes, checksum: 715de51ee1d8b3b7ffd295f5d7121216 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-04-26 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / The common bean (Phaseolus vulgaris) is grown in Brazil in various locations, soil and climatic conditions. The diseases are among the leading causes of losses in productivity of this legume, and the common bacterial blight (CBB) is the most important bacterioses that affects the culture. The resistance of CBB in common bean is a complex quantitative trait that results from the interaction of several genes. Genetic maps are tools that optimize the search for loci associated with this type of feature, and the most commonly used molecular markers available for this type of study are the SNPs (Single Nucleotide Polymorphism). In this sense, this study aimed to: (i) develop a robust genetic map for common bean using SNP markers and the RIL (Recombinant Inbreed Lines) mapping population derived from Ruda × AND 277; (ii) characterize this RIL population and their parents about the reaction to common bacterial blight in field and greenhouse; and (iii) identifying genomic regions (major genes and/or QTL) that control the bacterial blight in this population. We used 393 individuals of the Ruda × AND 277 RIL population, evaluated for reaction to CBB in two field trials in Ponta Grossa - PR, in the rain growing season of 2012 and 2014 and in an inoculation test at the greenhouse, in Santo Antônio de Goiás - GO. The population was genotyped with 5,398 SNP markers and mapping was performed using the R-OneMap and MapDisto programs. Statistical analyzes were performed in the Genes program, and the Scott-Knott method was used for averages groupingin R platform. The QTL analysis was conducted in QTLCartographer program. Using the chi-square test (1:1), 2,062 markers were selected for mapping. Three genetic maps with high strengt, saturation and resolution were built. Statistical analysis showed that there is genetic variability for the CBB resistance in the population of RILs. The QTL analysis identified 10 QTLs linked to resistance of CBB in the Ruda × AND 277 RIL mapping population, in the chromosomes PV01, PV02, Pv07, Pv09 and PV11, based on results from evaluations carried out in the field and greenhouse. The maps constructed for this population have high strength and resolution and may be used for future work on integrative mapping. The statistical analysis evidenced the quantitative character of resistance to CBB in common bean and showed that the parent Rudá has the CBB resistance alleles. It is expected that the markers linked to these QTLs identified can be used in future studies of marker assisted selection. / O feijoeiro-comum (Phaseolus vulgaris) é cultivado no Brasil em vários locais e diversas condições edafoclimáticas. As doenças estão entre as principais causas de prejuízos na produtividade dessa leguminosa, sendo o crestamento bacteriano comum (CBC) a principal bacteriose que afeta essa cultura. A resistência ao CBC no feijoeiro-comum é uma característica complexa, quantitativa, que resulta da interação de vários genes. Os mapas Genéticos são ferramentas que otimizam a busca de locos associados a esse tipo de característica, e os marcadores moleculares mais utilizados disponíveis para esse tipo de estudo são os SNPs (Single Nucleotide Polymorphism). Neste sentido, o presente trabalho teve como objetivos: (i) construir um mapa genético robusto para o feijoeiro-comum, utilizando marcadores SNP e a população de RILs (Recombinant Inbred Lines, ou linhagens endogâmicas recombinantes) derivada do cruzamento Rudá × AND 277; (ii) caracterizar esta população de RILs e seus genitores quanto à reação ao crestamento bacteriano comum, em campo e em casa de vegetação; e (iii) identificar regiões genômicas (genes de efeito principal e/ou QTLs) que controlam a reação ao crestamento bacteriano comum nesta população. Foram utilizados 393 indivíduos da população de RILs Rudá × AND 277, avaliados quanto à reação ao CBC em dois ensaios de campo em Ponta Grossa – PR, nas águas de 2012 e 2014, e em um ensaio de inoculação em casa de vegetação, em Santo Antônio de Goiás - GO. A população foi genotipada com 5.398 marcadores SNP e o mapeamento das RILs foi realizado utilizando os programas R-OneMap e MapDisto. As análises estatísticas foram realizadas no programa Genes, sendo o agrupamento de médias de Scott-knott realizado na plataforma R. A análise de QTL foi realizada no programa QTLCartographer. Por meio do teste de quiquadrado (1:1) foram selecionados 2.062 marcadores para o mapeamento. Foram construídos três mapas genéticos com elevada robustez, saturação e resolução. As análises estatísticas evidenciaram que há variabilidade genética para a característica de resistência ao CBC na população de RILs. A análise de QTL identificou 10 QTLs ligados à resistência ao CBC na população de RILs Rudá × AND 277 nos cromossomos Pv01, Pv02, Pv07, Pv09 e PV11 com base em dados obtidos a partir de avaliações em campo e casa de vegetação. Os mapas construídos para essa população apresentam elevada robustez e resolução e poderão ser utilizados para futuros trabalhos de mapeamento integrativo. As análises estatísticas evidenciaram o caráter quantitativo da resistência ao CBC em feijoeiro-comum e mostraram que o genitor Rudá possui alelos de resistência ao CBC. Espera-se que os marcadores ligados a esses QTLs identificados possam ser utilizados em futuros trabalhos de seleção assistida por marcadores.