[Truncated abstract] Establishment of vegetation and ecosystem functioning is central to the mitigation of environmental impacts associated with mining operations. This study investigated the ecophysiological functioning of mature plants in natural vegetation and applied this knowledge to diagnose problems affecting plant health and causes of poor plant cover at a mine-rehabilitation site. Ecophysiological parameters, including plant water relations and mineral nutrition, were studied in conjunction with soil physical, hydraulic and chemical properties. The natural ecosystem at the study location in the Great Sandy Desert is characterised by sand dunes and interdunes with distinct plant communities on each. One of the most notable features of the vegetation is the presence of large Corymbia chippendalei trees high on the dunes and relatively small scattered shrubs in the interdunes. Triodia grasses (spinifex), dominate the vegetation in both habitats but different species occur in each; T. schinzii is restricted entirely to the dunes, and T. basedowii occurs only in the interdunes. It was hypothesised that the deep sandy dunes afford greater water availability but lower nutrient supply to plants in this habitat compared with those occurring in the lower landscape position of the interdunes. Water-relations parameters (leaf water potentials, stomatal conductance, d13C) revealed that dune plants, particularly woody species, displayed higher water status and water use than closely related and often congeneric plants in the interdunes. Nutrient concentrations in soils were significantly higher in the interdunes, but concentrations in foliage were similar for related species between habitats. It is concluded that the dunes provide a greater store of accessible water than the soil profile in the interdunes. ... Following an experimental wetting pulse equivalent to a summer cyclone event, A. ancistrocarpa plants displayed significant increases in stomatal conductance, leaf water potential and sap velocity in lateral roots within three days of irrigation at the natural site and two days at the rehabilitation site. Secondary sinker roots originating from distal sections of lateral roots were evidently supplying water to maintain hydraulic function in laterals, thus enabling a fast pulse response. This was accentuated at the rehabilitation site where roots were confined closer to the surface. These results indicate that plants at the rehabilitation site are more dependent on small pulses of water and have less access to deep reserves than plants at the natural site. It is concluded that high runoff losses and insufficient soil depth are major factors contributing to plant water stress, and combined with the direct impacts of erosion, are largely responsible for plant death and ultimately poor plant cover. These issues can be alleviated if cover soil depth is increased to more than 0.5 m and slope angles are reduced to <12o. This study demonstrates the value of an ecophysiological approach for diagnosing problems affecting plant establishment at mine-rehabilitation sites. Furthermore, it has provided recommendations that will improve the rehabilitation strategy and lead to the development of a well vegetated, resilient ecosystem on a stable and non-polluting land form.

[Truncated abstract] Recovery of soil nutrients, microbial populations and carbon (C) and nitrogen (N) cycling processes are critical to the success of rehabilitation following major ecosystem disturbance. Bauxite mining represents a major ecosystem disturbance to the jarrah (Eucalyptus marginata) forest in the south-west of Western Australia. Mining has created a mosaic of mined areas in various stages of succession surrounded by non-mined forest areas. Initial site preparations within rehabilitation areas such as contour ripping alter soil structure (creation of mound and furrows) and over time also influence the distribution of vegetation and litter. Current performance criteria developed by industry, government and other stakeholders have determined that before post-bauxite mined areas of jarrah forest can be integrated back into normal forest management practises they should be functional and demonstrate resilience to normal forest disturbances such as fire. Furthermore, resilience should be of a manner comparable to non-mined analogue forest sites. Currently little is known of the resilience of microbial communities and C and N cycling in rehabilitation sites to normal forest disturbances such as prescription burning. As such, before rehabilitated jarrah forests can be successfully integrated into broad scale forest management regimes, a more thorough knowledge of the potential impacts of burning practises on the soil microbial community and C and N cycling processes in these systems is required. ... While there are similar rates of C and N cycling the underlying microbial community structure was distinctly different; implying a high degree of functional redundancy with respect to C and N cycling. Differences in the C and N cycling and structure of the microbial communities were likely to be due to differences in soil environmental conditions (i.e. soil alkalinity/acidity, soil moisture) and C substrate availability which influence the physiological status of the microbial community and in turn are related to successional age of the forests. Results also suggest that the measurement of CLPP can be a useful approach for assessment of changes in the functional ability of microbial communities. However, the interpretation of how well these rehabilitation forests have recovered heterotrophic abilities was greatly affected by the methodological approach used (e.g. MicroRespTM or Degens and Harris, 1997). Importantly, results from Chapter 4 and 5 suggested that the effects of a moderate prescription fire on C and N processes, CLPP and microbial community structure of 18 year old rehabilitation forests are likely to be short-lived (< 2 years). Furthermore, the effects of the moderate spring prescription fire were not large enough to decouple C and N cycling processes over the short-term (< 1 years) which suggests that by 18 years of age rehabilitation forests demonstrate comparable functional resilience to a moderate prescription burn.

[Truncated abstract. Please see the pdf format for the complete text. Also, formulae and special characters can only be approximated here. Please see the pdf version for an accurate reproduction.] This thesis examines the water use, ecophysiology and hydraulic architecture of Eucalyptus marginata (jarrah) growing on bauxite mine rehabilitation sites in the jarrah forest of south-western Australia. The principal objective was to characterise the key environment and plant-based influences on tree water use, and to better understand the dynamics of water use over a range of spatial and temporal scales in this drought-prone ecosystem. A novel sap flow measurement system (based on the use of the heat pulse method) was developed so that a large number of trees could be monitored concurrently in the field. A validation experiment using potted jarrah saplings showed that rates of sap flow (transpiration) obtained using this system agreed with those obtained gravimetrically. Notably, diurnal patterns of transpiration were measured accurately and with precision using the newly developed heat ratio method. Field studies showed that water stress and water use by jarrah saplings on rehabilitation sites were strongly seasonal: being greatest in summer when it was warm and dry, and least in winter when it was cool and wet. At different times, water use was influenced by soil water availability, vapour pressure deficit (VPD) and plant hydraulic conductance. In some areas, there was evidence of a rapid decline in transpiration in response to dry soil conditions. At the end of summer, most saplings on rehabilitation sites were not water stressed, whereas water status in the forest was poor for small saplings but improved with increasing size. It has been recognised that mature jarrah trees avoid drought by having deep root systems, however, it appears that saplings on rehabilitation sites may have not yet developed functional deep roots, and as such, they may be heavily reliant on moisture stored in surface soil horizons. Simple predictive models of tree water use revealed that stand water use was 74 % of annual rainfall at a high density (leaf area index, LAI = 3.1), high rainfall (1200 mm yr-1) site, and 12 % of rainfall at a low density (LAI = 0.4), low rainfall (600 mm yr-1) site, and that water use increased with stand growth. A controlled field experiment confirmed that: (1) sapling transpiration was restricted as root-zone water availability declined, irrespective of VPD; (2) transpiration was correlated with VPD when water was abundant; and (3) transpiration was limited by soil-to-leaf hydraulic conductance when water was abundant and VPD was high (> 2 kPa). Specifically, transpiration was regulated by stomatal conductance. Large stomatal apertures could sustain high transpiration rates, but stomata were sensitive to hydraulic perturbations caused by soil water deficits and/or high evaporative demand. No other physiological mechanisms conferred immediate resistance to drought. Empirical observations were agreeably linked with a current theory suggesting that stomata regulate transpiration and plant water potential in order to prevent hydraulic dysfunction following a reduction in soil-to-leaf hydraulic conductance. Moreover, it was clear that plant hydraulic capacity determined the pattern and extent of stomatal regulation. Differences in hydraulic capacity across a gradient in water availability were a reflection of differences in root-to-leaf hydraulic conductance, and were possibly related to differences in xylem structure. Saplings on rehabilitation sites had greater hydraulic conductance (by 50 %) and greater leaf-specific rates of transpiration at the high rainfall site (1.5 kg m-2 day1) than at the low rainfall site (0.8 kg m-2 day1) under near optimal conditions. Also, rehabilitation-grown saplings had significantly greater leaf area, leaf area to sapwood area ratios and hydraulic conductance (by 30-50 %) compared to forest-grown saplings, a strong indication that soils in rehabilitation sites contained more water than soils in the forest. Results suggested that: (1) the hydraulic structure and function of saplings growing under the same climatic conditions was determined by soil water availability; (2) drought reduced stomatal conductance and transpiration by reducing whole-tree hydraulic conductance; and (3) saplings growing on open rehabilitation sites utilised more abundant water, light and nutrients than saplings growing in the forest understorey. These findings support a paradigm that trees evolve hydraulic equipment and physiological characteristics suited to the most efficient use of water from a particular spatial and temporal niche in the soil environment.

Thesis (MScFor )--Stellenbosch University, 2003. / ENGLISH ABSTRACT: Species-rich Renosterveld vegetation does not return to old agricultural fields even after many years (10-20) of abandonment. In most cases the fields are slowly taken over by non-indigenous species, particularly alien pasture grasses and African lawn grass. While poor survival of indigenous seedlings due to competition, grazing or any other establishment constraints, might explain the failure of natural vegetation to colonise old fields, here I am testing the hypothesis that recolonisation by indigenous plant species is limited by seed dispersal. I have quantified changes in seed density with distance from the natural vegetation into old fields using seed traps and soil seed bank assessment. I have also looked at seed dispersal in the dung of large herbivores. Shrubs (with the exception of Eytropappus rhinocerotis) were poorly represented in the seed rain indicating that they might be seed limited. Dicerothamnus rinocerotis (a dominant shrub in Renosterveld) and certain geophytes (that were recovered in the soil bank) have proven that seed dispersal is not the primary constraint to their return to ploughed fields. Dicerothamnus rinocerotis produces a large number of seeds that are dispersed long distances into the old field and a number of geophytes were well represented in the soil bank. Large herbivores are dispersing a lot of herbaceous forbs in their dung but make very little contribution to the dispersal of indigenous tussock grass and shrubs. Seeds of indigenous Renosterveld tussock grasses (e.g Tribolium hispidum) were restricted to the edge of the natural vegetation. My research has shown that some indigenous seeds, particularly those dispersed in wind and through large mammals, do reach the old ploughed field. I therefore conclude that seed dispersal is not the primary constraint to the natural recovery of populations of some indigenous plant species on old fields, but may limit recovery of certain indigenous geophytes, grasses and shrubs. / AFRIKAANSE OPSOMMING: Spesie-ryke Renosterveld plantegroei hervestig nie op ou landerye nie, selfs ná baie jare (l0-20) van verlating. Oor die algemeen word hierdie grond stadig oorgeneem deur uitheemse spesies, veral uitheemse weidingsgrasse en kweekgras. Terwyl swak oorlewing van inheemse saailinge as gevolg van kompetisie, weiding of enige ander vestigingsbeperkinge, die mislukte vestiging van natuurlike plantegroei op ou benutte grond kan verduidelik, toets ek hier die hipotese dat hervestiging deur inheemse plantspesies deur saadverspreiding beperk word. Ek het veranderinge in saaddigtheid oor afstand vanaf die natuurlike plantegroei na ou landerye gekwantifiseer, deur gebruik te maak van saadopvangsvalle en sand saadbank beraminge. Ek het ook gekyk na saadverspreiding in die mis van groot herbivore. Struike (behalwe Eytropappus rhinocerotis) was swak verteenwoordig in die "saadreën", wat mag toon dat struike saadbeperk is. Eytropappus rhinocerotis ('n dominante struik in Renosterveld) en sekere geofiete (wat in die saadbank gevind is) het bewys dat saadverspreiding nie die hoofbeperking is vir hulle terugkeer na geploegde lande nie. Eytropappus rhinocerotis produseer 'n groot hoeveelheid saad wat oor groot afstande oor die ou landerye versprei word en 'n aantal geofiete was goed verteenwoordig in die saadbank. Groot herbivore versprei 'n groot hoeveelheid kruidagtige forbe in hulle mis, maar maak 'n baie klein bydrae tot die verspreiding van inheemse polgras en struike. Saad van inheemse Renosterveld polgrasse (bv. Tribolium hispidum) was beperk tot die some van die natuurlike plantegroei. My navorsing het getoon dat sommige inheemse saad, veral dié wat deur die wind en deur groot herbivore versprei word, wel die ou geploegde landerye bereik. Ek maak dus die gevolgtrekking dat saadverspreiding nie die hoofbeperking is vir die natuurlike herstel van populasies van sommige inheemse plantspesies op ou landerye nie, maar mag die herstel van sekere inheemse geofiete, grasse en struike beperk.

Globally, ecosystems provide services of almost twice the value of global gross national product (Costanza et al., 2006). The Buffalo City Municipality (BCM), South Africa contains biodiversity of national and international importance (Pierce, 2003; Pierce et al., 2005). Despite this, the municipality continues to experience loss of both urban and rural biodiversity (Buffalo City Municipality, 2006a). This study sought to determine the status of biodiversity, and the potential for ecosystem services to contribute to conservation, within the BCM. Biodiversity features, including ecosystem type, species of special concern and biodiversity processes, were identified and mapped using a GIS to produce a biodiversity priority index for the BCM. Current transformation status was then mapped to determine the level of ecosystem degradation within the BCM. Priority biodiversity areas as well as individual biodiversity features were spatially overlain against current transformation status and protected areas and analysed using a GIS to determine the level of degradation and protection of BCM biodiversity. In total 3.5 % of total BCM biodiversity was protected. Of the 24 ecosystem types, 11 (45%) had less than 1% under protection, while 16 (67%) had less than five percent protected. Not restorable areas, thus completely lost to biodiversity conservation, comprised just less than a quarter of the total BCM area while un-impacted areas comprised just 12.3%. Twenty five ecosystem services were identified as being provided by intact natural ecosystems within the BCM. The natural capital providing these services was identified and mapped to produce an ecosystem service index (ESI) using a GIS. This ecosystem service index and the biodiversity priority index were overlain to determine their level of correlation. Overall ESI correlation with priority biodiversity was weak although several individual ecosystem services, including carbon sequestration, showed correlation. Using the above data layers an implementation plan and conservation framework was proposed to assist the coordination of local conservation action within the BCM. It is concluded that ecosystem services are a potentially useful tool for conservationists at the local level seeking to ensure that biodiversity has relevance to and receives protection from broader society.

There is little understanding of the implementation efficiency and effectiveness of restoring plant invaded landscapes within operational contexts. South Africa's Working for Water (WfW) programme is arguably the most ambitious alien plant control programme in the world, yet little is known about its cost-effectiveness and the challenges it faces in linking poverty and environmental objectives. My first aim was to assess the cost-effectiveness of invasive plant removal, and the factors that underpin its effectiveness over large spatial and temporal scales. The second aim was to compare the accuracy of evidence-based findings with managers' experience-based beliefs, and to assess whether managers are willing to change their beliefs after being exposed to it. The third aim was to assess the costs and benefits of removal versus removal followed by active native re-vegetation. My final aim is to assess the challenges and lessons learnt by managers linking ecological restoration with poverty alleviation objectives, specifically within the public works model. My study area was focused primarily on two WfW river catchment projects in the western region of the Eastern Cape province. I adopted an interdisciplinary approach drawing from a range of methods such as observational studies, statistical modelling and interviews with managers. The key findings were that control efforts in the two catchment projects are largely inadequate owing to many sites being re-invaded and not enough resources being allocated to the catchments. It would take between 54 and 695 years to clear the respective catchments. In terms of cost-effectiveness, my results exceeded previous estimates by 1.5 to 8.6 times for each catchment project. After being exposed to the evidence-based findings, the managers did not change their beliefs when it came to forecasting the future effectiveness. I found that active native re-vegetation after removal of invasive plants is very costly and that priority should be given to understanding the effectiveness of the removal treatments on native species recovery. The managers cited significant challenges in effectively and efficiently meeting the programmes dual objectives. Based on a broader review of the public works literature I recommend WfW re-examine the type of public works they currently use.

A restauração ecológica visa o estabelecimento de ecossistemas semelhantes aos que originalmente ocupavam uma determinada área que foi degradada. Todavia, os resultados dos esforços envolvidos na restauração e no retorno dos processos ecológicos naturais são difíceis de serem medidos, pois faltam métodos apropriados. Os bioindicadores são ferramentas baratas e confiáveis para o diagnóstico das características ambientais e ecológicas, cujo princípio consiste em medidas simples de grupos biológicos especialmente sensíveis às mudanças no meio. Os Arthropoda que ocorrem no solo e na serapilheira, em especial as formigas, mediam processos ecológicos importantes e apresentam características que os tornam bioindicadores potenciais, mas seu uso ainda necessita de conhecimentos específicos. Assim, o presente estudo teve como objetivo testar o potencial indicador da comunidade de Arthropoda edáfico-epígea na restauração ecológica, com maior enfoque sobre as formigas. Foram avaliadas as comunidades de um gradiente sucessional formado por doze reflorestamentos com diferentes idades e três remanescentes florestais, localizados nas bacias dos rios Pardo e Mogi-Guaçu. O objetivo foi identificar quais os parâmetros que melhor refletem a resposta dessas comunidades ao desenvolvimento florestal. Os resultados indicaram que tanto a mirmecofauna como o restante da comunidade são influenciadas pelos estádios de desenvolvimento florestal. As comunidades variaram em composição e estrutura. A evolução das comunidades mostrou-se direcional, formando um padrão convergente de acordo com o avanço da sucessão. Os resultados indicaram forte efeito da sazonalidade e das técnicas de coleta na interpretação dos resultados. A comunidade edáfico-epígea encontrada no período seco mostrou-se especialmente sensível ao desenvolvimento florestal, enquanto a resposta da mirmecofauna foi mais evidente durante o período chuvoso. Os resultados indicaram também que as comunidades de diferentes regiões, Mogi-Guaçu e Ribeirão Preto, responderam ao processo sucessional, embora tenham apresentado diferenças na composição e no padrão de resposta. As análises com formigas classificadas em grupos funcionais indicaram que a resposta da mirmecofauna ao desenvolvimento florestal ocorre também no nível funcional. As mudanças nos padrões da comunidade e seus módulos estão relacionadas às mudanças nos filtros ambientais e à disponibilidade de nichos. Em conclusão, (1) a riqueza de espécies da fauna edáfico-epígea encontrada na serapilheira do período seco foi um bom preditor geral do desenvolvimento florestal; (2) a riqueza de grupos raros proporcionou o melhor modelo de prognóstico do estádio sucessional, indicando ainda que os reflorestamentos tendem a atingir patamares semelhantes aos encontrados nos remanescentes florestais por volta dos 27 anos após o plantio; (3) na comunidade edáfico-epígea, o avanço no desenvolvimento florestal levou à convergência entre padrões de composição, (4) enquanto na mirmecofauna a convergência ocorreu para os padrões de estrutura, sendo que em ambos os casos (3 e 4), quanto mais avançado o estádio sucessional, mais parecidos entre si são os padrões de locais diferentes; (5) a Equabilidade J das formigas amostradas por iscas decresceu ao longo do desenvolvimento florestal, sendo considerado também um indicador do processo sucessional (6) as classificações funcionais das formigas também permitiram boa predição dos estádios sucessionais, com destaque para formigas capturadas com iscas quando classificadas pelo sistema proposto por Andersen (1995), devido a boa relação custo/benefício. Portanto, foi confirmado o potencial bioindicador da mirmecofauna de comunidade edáfico-epígea geral, que podem ser utilizados como ferramentas de diagnóstico e monitoramento do processo sucessional. O uso mais apropriado deve ser feito por métodos comparativos, através de parâmetros provenientes de ecossistemas referência, ou por medidas repetidas ao longo do tempo, pela observação do deslocamento dos padrões da comunidade e interpretação da proporção dos grupos. / Ecological restoration objective is to promote the return of chemical, physical and biological natural properties as those similar to the native features as possible. However, the restoration projects cannot be well evaluated due to the fact that there are no appropriated tools to do that. Plantations using native species are a prominent technique used in São Paulo state, promoting the fast rising of a plant community, but it is very expensive and hard to measure if ecological processes are returning. Bioindicators are cheap tools to evaluate environmental conditions and ecological processes because they respond to all ecosystem characteristics acting together and the responses of a single group make possible to infer properties of the total biodiversity or the full ecosystem health. Epigeic and hipogeic Arthropoda, especially ants, are potential bioindicators, due to their high abundance, ubiquitousness and their straight relationship to some important ecological processes, even to other community components, but they have not been used because it lacks information about the local scale responses to successional process and which standards can be used as parameters. Hence, we have tried to understand the main standards and responses from soil and litter community, as well as the mirmecofauna responses, under a successional gradient constituted by twelve planted forests in different ages and three forest remainders (semi-deciduous forest), all located at the hydrographic basins, Pardo and Mogi-Guaçu, searching for trustful bioindicators of forest development. General fauna was sampled from litter and soil and ants were additionally sampled by attractive baits, using glucose and sardine. Results have showed that forest development makes influence on structure and composition of general communities and ant communities found in restoration sites, so that over time, these community parameters progressively have become more similar to those found in forest remainders, consisting in a directional shifting. Epigeic and hipogeic general fauna showed to be more sensitive to forest development through dry season, while ants presented critical responses in wet season. Communities from different regions were influenced by successional process but they have presented differences among fauna compositions. The analyses using ants classified in functional groups indicated that forest development makes influence also on fauna functional pattern. Groups occupying specialized niches tend to increase their relative amount throughout forest development. The shifting of ant community functionality may be related to changes in environmental filters plus the increase of niche availability through forest development. As conclusions, under experiment features (1) species richness of litter fauna of dry season is a good predictor of forest development; (2) the richness of rare taxa allowed the best prognosis about sucessional stage, also indicating that fauna found in forest plantations reaches similar fauna standards of forest remainders close to 27 years after the deployment; (3) over time, the composition of general edaphic-epigeic community found in restoration sites becomes more similar to composition of communities found in forest remainders, (4) while for ant communities, the structure standards found in restoration sites progressively become more similar of respective parameter found in forest remainders. (5) the Equitability J of ants communities sampled using baits decreases over forest development and it may be considered a bioindicator of sucessional process; (6) ant functional groups also provided good prediction of sucessional stages, highlighting ant sampling using baits and ant functional classification according to Andersen (1995), due to best benefit-cost ratio. For appropriated application of all bioindicators, the use of comparative techniques may be considered, including ecosystem references, and rather than it uses fixed values of fauna parameters (as fixed goals to be achieved), it is better to consider which parameters of the community are changing over time and if their tendencies are in accordance to the expected.

O acúmulo significativo de conhecimento sobre a ecologia de restauração nos últimos anos tem conduzido a mudanças na orientação dos programas de manejo e restauração florestal. A escolha ou criação de um modelo de restauração é um processo em constante aprimoramento, exigindo a necessidade de estudos que não apenas diminuam os custos envolvidos, mas também, que garantam a efetividade do objetivo proposto. Neste sentido, este trabalho testa novas metodologias de restauração visando a obtenção de informações técnicas sobre: A) a expressão do banco de sementes autóctone e alóctone na restauração florestal de diferentes situações de degradação antrópica.; B) o resgate de plântulas e indivíduos de espécies arbustivo-arbóreas regenerantes; C) a eficiência de um modelo de plantio que utiliza conceitos de Preenchimento e Diversidade e; D) a evolução da paisagem. Para análise dos bancos de sementes autóctone e alóctone foram feitos levantamentos da densidade e das espécies arbustivo-arbóreas regeneradas. No resgate de indivíduos de espécies arbustivo-arbóreas regenerantes, as plântulas e indivíduos jovens foram resgatadas em uma floresta e levadas para adapatação em viveiro, onde tiveram seus indivíduos e espécies contabilizados em termos de densidade e sobrevivência. No modelo de plantio estudado, as espécies foram reunidas em grupos de Preenchimento e Diversidade e tiveram a cobertura de copa de cada indivíduo quantificada. A evolução da paisagem foi realizada através de fotointerpretação e principalmente checagem de campo periódica. Os principais resultados dessas metodologias foram: A) As situações identificadas na Fazenda Intermontes apresentaram diferentes expressões de regeneração natural em função do número de indivíduos germinados banco de sementes autóctone. O aproveitamento dessa expressão poderá ser realizado através de metodologias diferenciadas de restauração para cada situação estudada: B) No estudo do Banco de sementes alóctone, a densidade dos indivíduos germinados se foi de 409.334 indivíduos/ha, revelando a possibilidade de uso no programa de restauração. Os indivíduos arbóreos apresentaram na área adubada um acréscimo de 137,3 % em sua altura média em relação aos indivíduos da área não adubada. C) No resgate de plântulas e indivíduos de espécies arbustivo-arbóreas regenerantes, a densidade dos indivíduos foi muito próxima para as duas épocas do ano, sendo 38.700 ind./ha e 37.900 ind./ha, mas com uma diferença significante para os indivíduos sobreviventes que apresentaram 16.500 ind./ha e 26.600 ind./ha para os meses de julho e dezembro de 2002, respectivamente. A técnica de resgate utilizada se mostrou viável uma vez que foi possível alcançar até 70,9% de sobrevivência para os indivíduos resgatados; D) No estudo sobre o modelo de plantio foram amostrados 143 espécies, demonstrando que a função do grupo da diversidade vem sendo mantida e aumentando as chances de efetivação dos processos de sucessão. Os resultados mostraram que o grupo de Preenchimento apresentou-se com uma porcentagem de cobertura significativamente maior que a do grupo de Diversidade, sendo 73,6% e 83,3% para 1,5 e 2,5 anos respectivamente. / Significant accumulation of know-how about restoration ecology has been changing forest restoration management programs’ trends during the last years. Choosing or elaborating a restoration model is a process that requires constant improvement and ecological studies that not only reduces the costs involved but also warrants the achievement of the aimed proposal. This study explores new restoration methodologies, searching for technical information about: A) autochthonous and allochthonous seed bank expression on forest restoration at different situations degraded by anthropogenic disturbances; B) seedlings and woody growing individuals rescue; C) efficiency of a planting model based on fulfillment and diversity concept; D) landscape development. The autochthonous and allochthonous seed bank were analyzed through regenerating woody species and density scouting. For the rescue of regenerating woody species, seedling and young individuals were taken from a forest and transferred to a plant nursery for adjustment, where number and species were counted regarding density and survival. In this studied planting model, species were organized and gathered into a fulfillment or diversity group, and each individual had its crown area quantified. Landscape development was analyzed by photointerpretation and specially by periodical field checking. Results from these methodologies showed that: A) Identified degraded situations at Intermontes farm displayed distinguished natural regeneration expression, influenced by the number of germinated seeds of the autochthonous seed bank. Advantage can be taken from these different outcomes by using distinct restoration methodologies for each studied situation. B) For the autochthonous seed bank study, germinated seed density was 409,334 ind./ha, indicating its potential role on restoration programs. Woody individuals at fertilized sites had a medium height enhancement of 137,7% when compared to those at non fertilized sites. C) For seedlings and woody growing individuals rescue, individual density was similar for both seasons (38700ind./ha and 37900ind./ha) but significantly distinct for individual survivor, with values of 16500ind./ha for July and 2600ind./ha for December. Rescue technique turned out to be a viable possibility since it achieved survival of 70,9% for rescued individuals. D) This planting model study registered 143 species, indicating that the diversity group’s role has been maintained and enhances the chance of successional process success. The fulfilling group had a significant higher percentage of crown area than the diversity group, with 73,6% and 83,3% for 1,5 and 2,5 years, respectively.

La restauration écologique est considérée comme un des moyens susceptibles d’enrayer la perte de la biodiversité. Les changements d’occupation du sol peuvent être des opportunités pour restaurer des écosystèmes dégradés par les activités agricoles. C’est notamment le cas du projet participatif du domaine du Cassaïre, situé dans le delta du Rhône, qui vise à recréer sur d’anciennes rizières des écosystèmes méditerranéens favorables à l’activité cynégétique. Deux écosystèmes sont plus particulièrement visés, les marais temporaires et les pelouses méso-xériques. Les objectifs de la thèse sont de mettre en évidence les principaux mécanismes concourant à l’installation d’une communauté végétale, de tester des techniques de restauration et d'en évaluer les conséquences pour les communautés végétales mais aussi pour d'autres compartiments de l'écosystème. En l’absence d’espèces cibles dans le pool régional d’espèces, l’introduction de ces espèces est nécessaire en addition de la restauration des conditions abiotiques. L’étrépage et le transfert de sol pour la communauté des marais permettent une augmentation des espèces cibles et de la similarité avec la communauté de référence. Cette technique apparaît moins pertinente pour la communauté d’invertébrés aquatiques. Le succès contrasté du transfert de sol souligne les risques à ne pas privilégier qu’un nombre restreint d’indicateurs de restauration, ne reflétant pas l’ensemble de l’écosystème. L’étrépage de sol suivi d’un transfert de foin semble être une combinaison pertinente pour recréer la communauté végétale de pelouses, même si les résultats obtenus, probablement en raison d’une compétition élevée, sont moins convaincants que pour les marais temporaires. Nos résultats obtenus pour la grande majorité en mésocosmes, s’ils ne concernent que les premières étapes de la restauration, mettent cependant en évidence des techniques de restauration qui paraissent pertinentes pour installer certaines composantes des deux écosystèmes de référence. Ces résultats, par leurs limites, suggèrent néanmoins de privilégier la conservation in situ des habitats naturels plutôt que de chercher à les restaurer après qu’ils aient été détruits. / Ecological restoration is considered as one approach to slow down the loss of biodiversity. Changes in land-uses may be an opportunity to restore ecosystems degraded by agricultural activities. This is the case of the participatory project of the Cassaïre site, located in the Rhône delta, which aims at recreating Mediterranean ecosystems favorable to hunting on former ricefields. Two ecosystems are targeted, temporary wetlands and meso-xeric grasslands. The aims of the thesis are to highlight the main drivers of plant community establishment, to test restoration techniques and to evaluate their effects on plant communities but also on other compartments of the ecosystem. In the absence of target species in the regional species pool, the introduction of these species is necessary in addition to the restoration of abiotic conditions. Topsoil removal and soil transfer for wetland communities allow an increase of target species and of similarity with the reference community. This technique appears to be less relevant for aquatic invertebrate community. The contrasted successful of soil transfer highlights the risks of favoring some indicators of restoration success, as they may not reflect the entire ecosystem. Topsoil removal and hay transfer seem to be a relevant combination to recreate grassland plant community, although the results obtained are less convincing than for temporary wetland, probably due to high competition. Our results, obtained in mesocosms, even if they relate only to the early stages of recovery, provide restoration techniques that seem relevant to establish some components of the two reference ecosystems. These results, by their limitations, however, suggest focusing on in situ conservation of natural habitats rather than trying to restore them after they were destroyed.Keywords: Biodiversity, Community ecology, Restoration

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