Both Candida albicans and lactobacilli are common colonizers of carious lesions in children and adolescents. The purpose of this study is to compare the velocity of acid production between C. albicans and several Lactobacillus species at different pH levels and concentrations of glucose. Washed, pure resting-cell suspensions were obtained by culturing a total of 28 oral isolates comprising the species C. albicans, Lactobacillus rhamnosus, Lactobacillus paracasei paracasei, Lactobacillus paracasei tolerans and Lactobacillus delbrueckii lactis. Acid production from glucose was determined at a constant pH of 7.0, 5.5, 5.0 and 4.0 by repeated titrations with NaOH in an automated pH-stat system. Acid formation rates of yeast and lactobacilli proved to be similar at both neutral and low pH, while in a moderately acidic environment C. albicans produced less acid than the lactobacilli. Ion chromatographic analysis of the cell-free medium after titration revealed pyruvate to be the predominant organic acid anion secreted by C. albicans. The proportion of organic acids to overall acid production by the yeast was below 10% at neutral conditions, in contrast to 42–66% at pH 4.0. Compared to lactobacilli, yeast required a concentration of glucose that was about 50 times higher to allow acid production at half the maximum speed. Considering the clinical data in the literature about the frequency and proportions of microorganisms present in early childhood caries lesions, the contribution of oral lactobacilli as well as C. albicans to overall microbial acid formation appears to be important. / Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.

Both Candida albicans and lactobacilli are common colonizers of carious lesions in children and adolescents. The purpose of this study is to compare the velocity of acid production between C. albicans and several Lactobacillus species at different pH levels and concentrations of glucose. Washed, pure resting-cell suspensions were obtained by culturing a total of 28 oral isolates comprising the species C. albicans, Lactobacillus rhamnosus, Lactobacillus paracasei paracasei, Lactobacillus paracasei tolerans and Lactobacillus delbrueckii lactis. Acid production from glucose was determined at a constant pH of 7.0, 5.5, 5.0 and 4.0 by repeated titrations with NaOH in an automated pH-stat system. Acid formation rates of yeast and lactobacilli proved to be similar at both neutral and low pH, while in a moderately acidic environment C. albicans produced less acid than the lactobacilli. Ion chromatographic analysis of the cell-free medium after titration revealed pyruvate to be the predominant organic acid anion secreted by C. albicans. The proportion of organic acids to overall acid production by the yeast was below 10% at neutral conditions, in contrast to 42–66% at pH 4.0. Compared to lactobacilli, yeast required a concentration of glucose that was about 50 times higher to allow acid production at half the maximum speed. Considering the clinical data in the literature about the frequency and proportions of microorganisms present in early childhood caries lesions, the contribution of oral lactobacilli as well as C. albicans to overall microbial acid formation appears to be important. / Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.

Durch die Flutung stillgelegter Tagebaue entstehen zahlreiche Seen in den ostdeutschen Braunkohlerevieren. Sie sind überwiegend groß, oligo- bis mesotroph, und ihr Litoral ist nur in geringem Umfang durch Makrophyten strukturiert. Viele von ihnen sind versauert oder versauerungsgefährdet. In einer Feldstudie wurden (1) die Auswirkung von Seemorphologie und Habitatstruktur auf die Fischgemeinschaft des Senftenberger Sees, eines 1050 ha großen mesotrophen Tagebausees, und (2) die Säuretoleranz einheimischer Fischarten unter den hydrochemischen Bedingungen der geogen versauerten Tagebauseen untersucht. Die Fischgemeinschaft des Senftenberger Sees wurde von Barschen (Perca fluviatilis) und Plötzen (Rutilus rutilus) dominiert, wobei die verschiedenen Barsch- und Plötzengrößenklassen ihre Habitate durch Nutzung verschiedener Tiefenbereiche des Litorals segregierten. Innerhalb der flachen Litoralbereiche waren juvenile Plötzen und juvenile Güstern deutlich stärker auf die wenigen Makrophytenbestände fixiert als juvenile Barsche. Die Habitatwahl der Barsche zeigt, dass diese in mesotrophen Seen wahrscheinlich eher von den guten Sichtverhältnissen als von der strukturellen Komplexität submerser Makrophytenbestände profitieren. Als Ursache für die unterschiedliche Habitatwahl juveniler Barsche, Plötzen und Güstern wird postuliert, dass diese generell verschiedene Habitatwahlstrategien verfolgen. Während der entscheidende Parameter für die Habitatwahl juveniler Barsche die Optimierung der Energieaufnahme ist, folgen juvenile Plötzen und Güstern vorrangig einer Räubervermeidungsstrategie und sind daher sehr viel stärker auf strukturreiche Bereiche angewiesen. Die kritischen pH-Untergrenzen für die einzelnen Fischarten entsprachen weitgehend den publizierten Ergebnissen aus regenversauerten Weichwasserseen. Vermutlich wurde in den Tagebauseen der Säurestress, den die erhöhte Al-Konzentration von bis zu 0,6 mg/L verursachte, durch die ebenfalls erhöhte Ca-Konzentration kompensiert. / Numerous post-mining lakes are created by flooding the pits remaining from open-cast lignite mining in eastern Germany. They are typically large, oligo- or mesotrophic and only sparsely structured by macrophytes. Many of them are acidified or at risk of acidification. The aim of this study was (1) to determine the effects of lake morphology and habitat structure on the fish community of Lake Senftenberg, a large mesotrophic post-mining lake, and (2) to determine the acid tolerance of indigenous fish species under the hydrochemical conditions of geogenically acidified post-mining lakes. The most abundant fish species in Lake Senftenberg were Eurasian perch (Perca fluviatilis) and roach (Rutilus rutilus). Different size classes of both species segregated along a depth gradient within the littoral zone. Unvegetated shallow littoral areas (depth 1-3 m) were mainly used by juvenile perch, whereas juvenile roach and juvenile white bream (Blicca bjoerkna) preferred macrophyte stands. This indicates that the structural complexity of submersed macrophytes is not the key factor favouring perch over roach under mesotrophic conditions. Instead, low turbidity is probably the most important factor. The habitat choice of juvenile perch, roach and white bream is suggested to reflect different habitat selection strategies. Whereas habitat selection of juvenile perch is predominantly driven by maximisation of food consumption rates, juvenile roach and juvenile white bream aim at minimising predation risk. Consequently, they are much more dependent on structurally complex habitats. The critical lower pH-thresholds of the fish species investigated in this study were similar to the values derived from soft water lakes affected by acid rain. The deleterious effects of elevated Al-concentrations (up to 0.6 mg/L) in the post-mining lakes were probably mitigated by high Ca-concentrations.