Made available in DSpace on 2014-06-11T19:29:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-01-31Bitstream added on 2014-06-13T19:59:29Z : No. of bitstreams: 1 silva_glm_me_ilha.pdf: 203923 bytes, checksum: e21b04a78900de2d1a49b1afed03c08c (MD5) / Dados de crescimento em pesagens mensais e bimestrais de bovinos da raça Guzerá, nascidos e/ou criados no período de 1977 a 2000, pertencentes ao rebanho da Fazenda de Ensino e Pesquisa do Câmpus de Ilha Solteira, UNESP, situada no município de Selvíria-MS, foram analisados visando a avaliação dos touros que foram utilizados nesse período. Tal avaliação foi feita por três procedimentos estatísticos: Diferença predita (DEP), Provável valor genético (PVG) e BLUP(Melhor estimador linear não viciado). Através do DEP e do BLUP, obteve-se classificação praticamente idêntica. Já a classificação pelo método PVG foi distinta das demais. / Data of growth in monthly and bimonthly of bovines of Guzera race, born and grown from 1977 to 2000, belonging to flock of farm of teaching and research of Campus of Ilha Solteira, UNESP, located in Selviria MS city, was analyzed certifying get the selection of bulls. The evaluation of bulls was done by three statistical procedures, Predicted Difference (PD), Probable Genetic Value (PGV) and Best linear unbiased prediction (BLUP). The ranking of the bulls by BLUP and PD were pretty much the same. Although, when PGV was used, the ranking was different of the other methods.

Orientador: Evaristo Bianchini Sobrinho / Banca: João Francisco Pereira Bastos / Banca: Décio Barbin / Resumo: Dados de crescimento em pesagens mensais e bimestrais de bovinos da raça Guzerá, nascidos e/ou criados no período de 1977 a 2000, pertencentes ao rebanho da Fazenda de Ensino e Pesquisa do Câmpus de Ilha Solteira, UNESP, situada no município de Selvíria-MS, foram analisados visando a avaliação dos touros que foram utilizados nesse período. Tal avaliação foi feita por três procedimentos estatísticos: Diferença predita (DEP), Provável valor genético (PVG) e BLUP(Melhor estimador linear não viciado). Através do DEP e do BLUP, obteve-se classificação praticamente idêntica. Já a classificação pelo método PVG foi distinta das demais. / Abstract: Data of growth in monthly and bimonthly of bovines of Guzera race, born and grown from 1977 to 2000, belonging to flock of farm of teaching and research of Campus of Ilha Solteira, UNESP, located in Selviria MS city, was analyzed certifying get the selection of bulls. The evaluation of bulls was done by three statistical procedures, Predicted Difference (PD), Probable Genetic Value (PGV) and Best linear unbiased prediction (BLUP). The ranking of the bulls by BLUP and PD were pretty much the same. Although, when PGV was used, the ranking was different of the other methods. / Mestre

A estratégia de criação de grupos genéticos aditivos é uma técnica que permite que animais de paternidade desconhecida tenham seus valores genéticos preditos de forma mais adequada quando incluídos em programas de melhoramento genético. O modo como esses grupos são formados é ainda arbitrário, o que torna importante o estudo de metodologias de formação de grupos genéticos aditivos (GG) visando a uma estrutura apropriada para as avaliações dos rebanhos em programas de melhoramento genético animal. O objetivo do trabalho foi definir a estrutura de grupo genético adequada às avaliações genéticas, comparando-as em relação às mudanças efetivas na eficiência do processo seletivo dos animais com paternidade desconhecida. As características estudadas foram: peso ao desmame, peso ao sobreano, ganho de peso pós-desmama, perímetro escrotal ao sobreano e escore visual de musculosidade ao sobreano. Três cenários foram simulados a partir de um banco de dados composto apenas por animais com pedigree completo (grupo controle). O primeiro teve 30% dos animais com identificação de pai apagada, o segundo 50% e um terceiro com 70%. As estratégias de formação de GG foram: a fazenda de nascimento do animal com paternidade desconhecida; o ano de nascimento (SAFRA) e a concatenação de ano de nascimento e fazenda de nascimento (FAZSAFRA). Os componentes de variância foram calculados para o banco controle pelo software VCE e os valores genéticos foram preditos pelo software PEST, utilizando duas estruturas de modelo animal que se diferenciaram pela inclusão ou não do efeito fixo de GG. A definição da estrutura de grupo genético aditivo adequado à avaliação genética dos animais foi baseada na eficiência de seleção e na comparação entre os valores genéticos preditos quanto aos seus valores absolutos e quanto à classificação dos animais, sendo que animais do grupo controle foram assumidos como tendo o máximo em resposta à seleção genética. Os resultados demonstraram que os grupos genéticos aditivos proporcionam uma melhora na predição genética dos animais com paternidade desconhecida. As estratégias que proporcionaram valores mais próximos aos do grupo controle foram SAFRA e FAZSAFRA e, mesmo com similaridade de valores, a estratégia SAFRA foi superior na seleção dos melhores animais. / The structure of genetic groups is a technique that allows that animals with unknown paternity be included in breeding programs. The ways these groups are formed are still arbitrary, which makes it important to study formation methodologies of genetic groups aiming an appropriate framework for the evaluation of livestock in animal breeding programs. Therefore, the aim of this study was to define the structure of genetic group suited to genetic evaluations, comparing them regarding changes in the effective efficiency of the process of animals with unknown parentage. The characteristics studied were: weaning weight, post-weaning weight, post-weaning weight gain, scrotal circumference at 18 months of age and visual muscularity score at 18 months of age. Three scenarios were simulated from a database consisting only of complete pedigree animals (control group). The first had 30% of animals with identification of father off, the second with 50% and a third with 70%. The training strategies additive genetic groups were: the farm of birth of the animal with unknown parentage; birth year (SAFRA) and the concatenation of year of birth and birth farm (FAZSAFRA). The variance components were calculated for the data bank control program by VCE and breeding values were predicted using PEST software, with two structures of animal models that differ by the inclusion or not of the fixed effect of additive genetic group. The definition of the structure of genetic group suitable for genetic evaluation of animals was based on the efficiency of selection and comparison of estimated breeding values to the \"control\" breeding values and the classification of animals, while control animals were assumed to have the maximum response to selection whereas the choice of any other group of animals results in a reduction thereof. The results demonstrated that the additives genetic groups provide an improvement in the genetic prediction of animals with unknown paternity. Strategies that provided values closer to those of the control group were SAFRA and FAZSAFRA and even with similarity values, strategy SAFRA was superior in selecting the best animals.

Research to determine the impact of PTA<SUB>SCS</SUB> on incidence of mastitis, daughter response to infection, and other measures of daughter performance was conducted using data on 1st, 2nd, and 3rd lactation Holsteins obtained from the Virginia Tech herd and from VA DHI herds. Overall correlation of PTA<SUB>SCS</SUB> to lactation average SCS ranged from 0.13 to 0.17 across all data sets. Correlations between PTA<SUB>SCS</SUB> and 1st lactation SCS measures were higher than those between PTA<SUB>SCS</SUB> and SCS in later parities, but higher correlations were found between 2nd and 3rd lactation SCS measures than between 1st and later parities. Correlation of lactation average SCS and incidence of clinical mastitis was 0.41. Regression of lactation average SCS and averages of test day SCS measures on PTA<SUB>SCS</SUB> was significant in 1st, 2nd, and 3rd lactations. All significant relationships were linear and equal or close to 1.0. Relationships between PTA<SUB>SCS</SUB> and number of cases of clinical mastitis (.79), number of treatments (2.0), number of days treated (7.0), changes in SCS from beginning to end of a lactation (-.26), and the slope of changes in test day SCS with DIM (5.9x10-4) were significant only in 1st lactation. No significant relationships between PTA<SUB>SCS</SUB> and measures of clinical mastitis or variation in test day SCS measures were found in 2nd or 3rd lactations. Heavy cull rates imposed on 1st lactation cows in the Virginia Tech herd explained lack of significance in the later parities in the herd study, but results in following analyses indicated that measures of SCS in 1st and later parities may be two different, but correlated, traits. The greatest impact of PTA<SUB>SCS</SUB> on measures of daughter performance and profit was the negative relationship between PTA<SUB>SCS</SUB> and herd life. Increased PTA<SUB>SCS</SUB> resulted in the decreased ability to survive involuntary culling, and thus decreased opportunity for lifetime yield and profit. Selection on PTA<SUB>SCS</SUB> should be an effective method of reducing incidence of clinical mastitis, lactation average SCS, and variation in SCS, or response to infection. The response, however, may be different in 1st lactation than in later parities. / Master of Science

Female mammals are known to exhibit pregnancy failure when exposed to various stimuli during the implantation phase of pregnancy. When newly inseminated female mice are exposed to novel males in the absence of the sire, implantation is disrupted. This phenomenon is known as the Bruce effect. When females are exposed to novel males in the presence of the sire, pregnancy failure does not occur. This latter effect has been referred to as the sire protection effect. In these studies we examine the nature of female and sire behaviour in the context of novel males. Female interactions with novel males significantly decrease in the presence of the sire. This reduction in female-novel male interaction in seen irrespective of whether sires are free or confined within the female's cage. Novel-male exposed females exhibit pregnancy failure and this pregnancy block is removed when either free of corral-confined sires remain present in the cage. Finally, sires are highly motivated to engage in aggressive conflicts with novel males both in the presence and absence of the pregnant female. Sires were observed to behave aggressively towards novel males through a wire-mesh grid and in this context were able to inflict severe wounding upon novel males through the wire-mesh partition. In a direct exposure paradigm, sires were also witnessed to exhibit this aggression and were found to initiate and win all conflicts with novel males. The sire protection affect is likely to involve a complex of both pheromonal and behavioural cues. Sire aggression towards novel males, pheromonal communication and limited behavioural interaction between the sire and the female, and reduced femalenovel male interaction are all likely components of the effect. / Thesis / Master of Science (MSc)

Texte remanié de : Th. Ph. D. : University of Hull : 1985. / Le titre initial de la thèse était : "Form and meaning in two thirteenth-century verse romances : "La Manekine" and "Jehan et Blonde" by Philippe de Rémi.

During the work, results of ten sires of beef breeds had been observed in OPB Cunkov s.r.o. Over the years 2004 to 2007, 590 pieces of animals was evaluated. The highest number of all the evaluated sires was at the breed charolais (211 pieces), with average daily gain of 1883 grams, which was the highest daily gain from all the breeds evaluated. Of all the number of bulls included, 81,7% of them was included in breeding process and 18,3% of them was negative selected. Rearing house is an important means the other progress of beef cattle breeding.

Um dos grandes problemas que afetam a produção in vitro de embriões é a variação entre touros em relação à fertilidade e o maior nascimento de embriões do sexo masculino. Muitos fatores podem alterar a razão de 1:1 entre os gêneros tanto na produção in vitro de embriões, como no método de Inseminação Artificial. No sentido de tentar alterar esta proporção sexual in vitro, estudamos em um primeiro momento, a variação entre touros no desenvolvimento de embriões machos e fêmeas, distribuídos nas fases de blastocisto jovem, blastocisto, blastocisto expandido e blastocisto eclodido, bem como, a proporção macho: fêmea em relação a fase do blastocisto. Para tanto, oócitos foram coletados de ovários oriundos de matadouro e maturados em meio de maturação em incubadora por 24h. Espermatozóides viáveis de 17 touros do Programa de Melhoramento Genético da Raça Nelore, obtidos por centrifugação em gradiente de Percoll, foram utilizados para Fecundação in vitro. Após 12h, os supostos zigotos foram cultivados em meio de cultivo e células do cumulus em incubadora. Ao sétimo dia após a fertilização in vitro foi feita a seleção dos blastocistos viáveis que foram sexados com a utilização de primers Y-específico bovino e autossômico bovino, com visualização dos fragmentos amplificados em gel de agarose. Posteriormente, para obtenção de maior conhecimento a respeito dos animais testados, coletamos dados de progênie a campo destes animais e verificamos a proporção sexual de seus filhos nascidos pelo método da inseminação artificial, monta natural e possíveis correlações com características quantitativas. Diferenças entre touros foram observadas em relação à proporção dos sexos para as fases de blastocisto inicial e blastocisto expandido. Não foi observado desvio da proporção sexual em relação ao touro utilizado na produção in vitro de embriões e progênie a campo. Nosso dados sugerem que é possível alterar a proporção macho:fêmea em sistemas de produção in vitro de embriões pela seleção do estágio do blastocisto para transferência, e ainda, sugerem que touros apresentam variação em relação ao desenvolvimento in vitro de embriões machos e fêmeas de acordo com o estágio do blastocisto. / Some of the main problems that affect the in vitro production of embryos is the variation among bulls in relation to fertility and the greater birth of male embryos. Many factors can change the ratio 1:1 between the genders, both in the in vitro production of embryos and in the method of artificial insemination. To try to change this sex ratio in vitro, we studied at first, the variation among bulls in the development of female and male embryos, the blastocyst-stage distributed in young blastocyst, blastocyst, expanded blastocyst and hatched blastocyst and the proportion male: female in relation to the blastocyst stage. For this, oocytes were collected from slaughterhouse ovaries and matured in maturation medium in an incubator for 24h. Viable sperm from 17 bulls belonging to the Breeding Program of Nellore, obtained by Percoll gradient centrifugation were used for in vitro fertilization. After 12 hours, the presumptive zygotes were cultured in culture medium and cumulus cells in an incubator. After 168 hours of in vitro fertilization the viable embryos in the blastocyst stage were classified. The determination of the sex of the blastocysts was performed by Polymerase Chain Reaction (PCR) using a Y-specific sequence and bovine autosomal sequence visualized in agarose gel. Data analysis was conducted using the Statistical Analysis System software. Later, to obtain more knowledge about the animals tested, data was collected from the field progeny of these animals and established the sex ratio of the offspring produced by the method of artificial insemination, natural mating and possible correlations with quantitative traits. Differences between bulls were observed relating to the proportion of genders at the initial stages of blastocyst and expanded blastocyst. No deviation was observed in the sex ratio in relation to the bull used for in vitro production of embryos and offspring in the field. Our data suggest that it is possible to change the male to female proportion in production systems of in vitro embryos through the selection of the blastocyst stage for transfer, and also suggest that bulls show variation in relation to the in vitro development of male and female embryos, depending on the blastocyst stage.

Um dos grandes problemas que afetam a produção in vitro de embriões é a variação entre touros em relação à fertilidade e o maior nascimento de embriões do sexo masculino. Muitos fatores podem alterar a razão de 1:1 entre os gêneros tanto na produção in vitro de embriões, como no método de Inseminação Artificial. No sentido de tentar alterar esta proporção sexual in vitro, estudamos em um primeiro momento, a variação entre touros no desenvolvimento de embriões machos e fêmeas, distribuídos nas fases de blastocisto jovem, blastocisto, blastocisto expandido e blastocisto eclodido, bem como, a proporção macho: fêmea em relação a fase do blastocisto. Para tanto, oócitos foram coletados de ovários oriundos de matadouro e maturados em meio de maturação em incubadora por 24h. Espermatozóides viáveis de 17 touros do Programa de Melhoramento Genético da Raça Nelore, obtidos por centrifugação em gradiente de Percoll, foram utilizados para Fecundação in vitro. Após 12h, os supostos zigotos foram cultivados em meio de cultivo e células do cumulus em incubadora. Ao sétimo dia após a fertilização in vitro foi feita a seleção dos blastocistos viáveis que foram sexados com a utilização de primers Y-específico bovino e autossômico bovino, com visualização dos fragmentos amplificados em gel de agarose. Posteriormente, para obtenção de maior conhecimento a respeito dos animais testados, coletamos dados de progênie a campo destes animais e verificamos a proporção sexual de seus filhos nascidos pelo método da inseminação artificial, monta natural e possíveis correlações com características quantitativas. Diferenças entre touros foram observadas em relação à proporção dos sexos para as fases de blastocisto inicial e blastocisto expandido. Não foi observado desvio da proporção sexual em relação ao touro utilizado na produção in vitro de embriões e progênie a campo. Nosso dados sugerem que é possível alterar a proporção macho:fêmea em sistemas de produção in vitro de embriões pela seleção do estágio do blastocisto para transferência, e ainda, sugerem que touros apresentam variação em relação ao desenvolvimento in vitro de embriões machos e fêmeas de acordo com o estágio do blastocisto. / Some of the main problems that affect the in vitro production of embryos is the variation among bulls in relation to fertility and the greater birth of male embryos. Many factors can change the ratio 1:1 between the genders, both in the in vitro production of embryos and in the method of artificial insemination. To try to change this sex ratio in vitro, we studied at first, the variation among bulls in the development of female and male embryos, the blastocyst-stage distributed in young blastocyst, blastocyst, expanded blastocyst and hatched blastocyst and the proportion male: female in relation to the blastocyst stage. For this, oocytes were collected from slaughterhouse ovaries and matured in maturation medium in an incubator for 24h. Viable sperm from 17 bulls belonging to the Breeding Program of Nellore, obtained by Percoll gradient centrifugation were used for in vitro fertilization. After 12 hours, the presumptive zygotes were cultured in culture medium and cumulus cells in an incubator. After 168 hours of in vitro fertilization the viable embryos in the blastocyst stage were classified. The determination of the sex of the blastocysts was performed by Polymerase Chain Reaction (PCR) using a Y-specific sequence and bovine autosomal sequence visualized in agarose gel. Data analysis was conducted using the Statistical Analysis System software. Later, to obtain more knowledge about the animals tested, data was collected from the field progeny of these animals and established the sex ratio of the offspring produced by the method of artificial insemination, natural mating and possible correlations with quantitative traits. Differences between bulls were observed relating to the proportion of genders at the initial stages of blastocyst and expanded blastocyst. No deviation was observed in the sex ratio in relation to the bull used for in vitro production of embryos and offspring in the field. Our data suggest that it is possible to change the male to female proportion in production systems of in vitro embryos through the selection of the blastocyst stage for transfer, and also suggest that bulls show variation in relation to the in vitro development of male and female embryos, depending on the blastocyst stage.

Die Arbeit liefert eine umfassende Beschreibung der Managementmethoden von Milcherzeugerbetrieben unter dem Fokus Herdenfruchtbarkeit. Die Studie analysiert Einflüsse des Managements auf Reproduktions- und Milchleistung und verfolgt hierzu einen bio-sozialen Forschungsansatz. Die Daten wurden 2007 in Interviews mit Herdenmanagern und Stallrundgängen anhand von Fragebögen in 84 v.a. Brandenburger Milcherzeugerbetrieben erhoben. Die Fragen betrafen u.a. Haltungsumwelt, Stressvermeidung, Herden-, Reproduktions- und Personalmanagement. Die Leistungsdaten der Herden entstammen der Milchleistungsprüfung 2007. Die Datenanalyse umfasste qualitative und quantitative Methoden. Abhängige Variablen waren Zwischenkalbezeit (ZKZ) und 305-Tage-Milchleistung (ML). Im Mittel betrug die Herdengröße 306,3 Kühe (+/-238,3), die ZKZ 413,2d (+/-18,73) und die ML 8555kg (+/-1132,9). Die ZKZ sank tendenziell mit steigender ML (r=-0,188, p=0,10). Die ML stieg mit der Herdengröße (r=0,29, p=0,01). Die Laufgänge (LG) waren in 71,25% der Betriebe nass und rutschig. Hier war die ZKZ tendenziell länger (+16,6d, p=,055) als bei trockenen, rutschfesten LG. Die Liegeflächen (LF) waren in 29,9% der Betriebe trocken und elastisch. Hier war die ML höher (+1110kg, p=0,011) als bei nassen, harten LF. Eine eindeutige Zuordnung der Verantwortung für die Brunstkontrolle wirkte tendenziell positiv auf die ZKZ (-6,3d, p=0,129). Akademisch ausgebildete Herdenmanagerinnen erzielten eine höhere ML als gleichqualifizierte Männer (+752,9kg, p=0,005) bei gleicher ZKZ und damit eine günstigere Relation von Reproduktions- und Milchleistung. Motivierung durch materielle und soziale Anreize bzw. Verantwortungsübertragung und gute Kommunikation war mit einer höheren ML verbunden als Motivierung durch Leistungslohn (+ ca. 1000kg, p=0,009). Die Studie belegt die Notwendigkeit verbesserter Haltungsbedingungen und bietet eine Grundlage für vertiefende Studien zum Personalmanagement in Milchviehbetrieben. / This study provides a comprehensive characterization of current management methods of dairy farms, focusing on herd fertility. Relations of management factors to fertility and milk performance are analyzed following a bio-social approach. In 2007 a questionnaire survey including face-to-face interviews and direct observations was conducted in 84 East German dairy farms, mostly located in Brandenburg. Questions referred to housing, stress prevention, and management of herds, reproduction and personnel. Herd performance data stem from milk performance testing in 2007. Data analysis combined qualitative and quantitative methods. Calving interval (CI) and 305-day-milk yield (MY) were used as dependent variables. Mean values of herd size, CI and MY were, respectively, 306.3 cows (+/-238.3), 413.2d (+/-18.73d) and 8555kg (+/-1132.9). CI tended to decrease with increasing MY (r=-0.188, p=0.10). MY increased with rising herd size (r=0.29, p=0.01). Floors were wet and slippery in 71.25% of farms. In these farms CI tended to be longer (+16.6d, p=0.055) compared to farms with dry and non-slippery floors. Lying areas were in 29.9% of the farms dry and flexible. Here MY was higher (+1110kg, p=0.011) than in farms with wet and hard lying areas. A clear assignment of responsibility for heat detection showed a trend of decreasing CI (-6.3d, p=0.129). Female herd managers with academic qualification achieved a higher MY than likewise qualified men (+752.9kg, p=0.005), with no difference in CI. Thus, herds managed by highly qualified women showed a better MY:CI ratio. Employee motivation by material and social incentives or by allocating responsibility to workers and pursuing good communication was related to higher MY than motivation by performance pay alone (+1000kg, p=0.009); CI remained unaffected. Performance pay had no positive effect on targeted parameters. Results underscore the need for improved housing and recommend further study into personnel management in dairy farms.