Durch den Fortschritt der Digitalisierung sind Computer zu einem wesentlichen Bestandteil des Alltags ihrer Nutzer geworden. Folglich wächst die Bedeutung der nutzerzentrierten Entwicklung von soziotechnischen Systemen, die neben dem technischen System auch die komplexen Anforderungen des sozialen Systems berücksichtigt. Die vorliegende Dissertation untersucht in diesem Zusammenhang die nutzerzentrierte Gestaltung der Mensch-Computer Interkation (MCI) in soziotechnischen Systemen, damit sie den Nutzer bestmöglich und intuitiv unterstützt. In diesem Zusammenhang werden (a) die Integration der interdisziplinären Zusammenarbeit, die für die nutzerzentrierte Entwicklung benötigt wird, in den Entwicklungsprozess, (b) die nutzerzentrierte Erhebung von Anforderungen an die MCI, (c) die Gestaltung der MCI in soziotechnischen Systemen basierend auf den identifizierten Anforderungen und (d) die Evaluation soziotechnischer Systeme hinsichtlich der Erfüllung der Nutzeranforderungen untersucht. Durch die Erhebung von Anforderungen und Entwicklung von Gestaltungsprinzipien, Vorgehensmodellen und Prototypen sowie die rigorose Evaluation der Ergebnisse trägt die Dissertation zur gestaltungsorientierten Wirtschaftsinformatik bei und bietet der Praxis wertvolle Erkenntnisse über Nutzeranforderungen, Nutzungsverhalten und Gestaltungsprinzipien, die zur Realisierung und Verbesserung der MCI beitragen.

Sympathetic stimulation occurs in the heart after injuries such as ischemia/reperfusion (I/R) and myocardial infarction and affects myocardial remodeling. Prolonged sympathetic stimulation can result in myocardial dysfunction through its effects on cardiac myocyte apoptosis and myocardial fibrosis. Ubiquitin (UB) is well known for its role of tagging old or damaged proteins for degradation via the UB-proteosome pathway. The role of exogenous UB however, is not fully understood. Previously, our lab showed that β-adrenergic receptor (β-AR) stimulation increased levels of extracellular UB in the conditioned media of adult rat ventricular myocytes and that UB inhibits β-AR-stimulated apoptosis. This study investigates the role of extracellular UB after myocardial I/R injury in terms of infarct size, function, inflammation and proteomic changes in vivo as well as the effects of extracellular UB on cardiac fibroblast function in vitro. First, we validated a method of consistently measuring real-time myocardial ischemia and reperfusion in vivo. Second, cardiac function was studied 3 days post I/R injury in the presence or absence of UB infusion. Echocardiographic analysis determined UB infusion increased cardiac function after I/R injury in terms of ejection fraction and fractional shortening. UB decreased infarct size and infiltration of inflammatory cells including neutrophils and macrophages as well as reduced activity of neutrophils. UB increased protein levels of matrix metalloproteinase (MMP)-2 and transforming growth factor-β1 and increased activity of MMP-9. Third, in adult rat primary cardiac fibroblasts, we demonstrate that extracellular UB interacts with CXCR-4. UB treatment decreased serum-mediated increases in fibroblast proliferation and enhanced the contraction of fibroblast-populated collagen gels. Thus, extracellular UB likely interacts with CXCR-4 to influence fibroblast function and proliferation. Additionally, UB influences cardiac remodeling in terms of heart function, infarct size, inflammatory response and proteomic profile.

Adult obesity is associated with increased morbidity and mortality. Increasing success in weight loss maintenance will decrease the prevalence of overweight and obesity, and therefore help control the adverse health effects of excess weight. Much is known about the behavioral characteristics of successful long-term weight loss maintenance, but less is known about the cognitive processes behind weight loss maintenance. The purposes of this study were to (1) identify differences in visual attention to high-energy dense foods between individuals who are normal weight, weight loss maintainers, and overweight/obese in a high-risk (food-buffet) situation; (2) to evaluate differences in food choices from a food buffet between weight status groups; (3) to analyze correlations between food attention and food choice across weight status groups. No significant differences were found between groups with respect to food attention or food choice. Overall, findings from this study may have been limited by methodology, technology, and sample size. Future research is needed to better understand the interaction of cognitive processes and weight loss maintenance.

Sustaining behaviour change is fundamental for the effective uptake of policies and practices aimed at improving individual and collective health, yet it can be very difficult for individuals to adhere to new desired health-related behaviours. A prospective solution is to focus on instilling these behaviours as a habit. Once instilled as a habit, behaviours are performed automatically given specific cues, and they relieve the cognitive stress of having to make a volitional decision towards performing the behaviour - making it more resistant to relapse. Several information systems (IS) are being proposed to help individual users instil target behaviours as habits so that they can be performed even when intentions shift. However, these systems tend to be designed in an ad hoc manner and, as a result, their effectiveness can vary substantially. To better guide the design of information systems that can support users in forming health-related behaviours as a habit, we define a subclass of systems called Habit Formation Support Systems (HFSS) and adopt the design science research approach to develop two artifacts: (1) a design-relevant theory explaining and predicting how IS-supported habit formation can be achieved called the Theory for the Formation of IS-Supported Habits (T-FISSH); and (2) a suite of design principles to guide the design of systems that can effectively support users in forming a desired health-related behaviour as a habit. We contend that habit theory and IS continuance theory can be used to anchor the development of the two artifacts. The T-FISSH was refined and validated using exploratory and confirmatory focus groups with academics and health behaviour change practitioners respectively. The design principles were refined with systems design and development professionals and validated through a reusability check that involved a design activity and reusability questionnaire. The theoretical contribution of the thesis lies in moving the habit formation knowledge base into the design realm through the development of a design-relevant explanatory/predictive theory. From a practical perspective, this research presents a suite of theory-anchored design principles that are intended to guide the design and development of systems that can support users in forming desired health-related behaviours as a habit.

No description available.

<p>Cytochrome c (Cyt c) is a heme-containing protein that is a component of the electron transport chain as well as the mitochondrial apoptotic pathway. It is released from the mitochondrial intermembrane space to the cytosol during apoptosis and is also thought to be a biomarker for cancer and liver disease. Therefore, an efficient Cyt c biosensor would be a very useful tool for studying apoptosis. Here we show the process of development of Cyt c-dependent aptazymes, derived by <em>in vitro </em>selection. These aptazymes consist of 3 components: 1) a substrate with a cleavage site that consists of a single ribonucleotide flanked by a fluorophore and quencher; 2) a DNAzyme (catalytic DNA) motif capable of cleaving the substrate; 3) an aptamer, a short piece of single- stranded DNA that can specifically bind Cyt c. When Cyt c is absent, the aptamer occludes the catalytic core of the DNAzyme and the fluorophore of the intact substrate is quenched. However, when Cyt c is present, the aptamer binds Cyt c, allowing the DNAzyme to cleave the embedded ribonucleotide, separating the fluorophore and quencher, resulting in a fluorescent signal. Simulations of <em>in vitro </em>selection of Cyt c- dependent aptazymes were also performed. The simulations revealed several methods that can improve the success rate of future <em>in vitro </em>selections of aptazymes.</p> <p>Further analysis of the previously derived DNAzyme DEC22-18 was also performed. A detailed understanding of this DNAzyme will allow it to be developed into a biosensor.</p> / Master of Science (MSc)

INTRODUCTION: The detection of a second inorganic phosphate (Pi) resonance, a possible marker of mitochondrial content in vivo, using phosphorus magnetic resonance spectroscopy (31P- MRS) at 3T is technically challenging, which may prevent its reproducible quantification. PURPOSE: To determine the reproducibility of resting alkaline inorganic phosphate (Pialk) measurement using 31P-MRS in human skeletal muscle at 3 tesla (T). METHODS: Resting 31P- MRS of the quadriceps muscles was acquired on two separate visits, within seven days, in 13 healthy, sedentary to moderately active young adults using a whole-body 3T MR system. Measurement variability related to coil position, shimming procedure, and spectral analysis were also quantified. 31P-MRS data were acquired with a 31P/1H dual-tuned surface coil positioned on the quadriceps using a pulse-acquire sequence. Test-retest absolute and relative reproducibility were analyzed using coefficient of variation (CV) and intra-class correlation coefficients (ICC), respectively. RESULTS: Pialk demonstrated a within-subject reproducibility marginally greater then the 10% cutoff (CV: 10.6 ± 5.4%; ICC: 0.80), but still appropriate given its small concentration in relation to other 31P metabolites. Proximo-distal change in coil positioning along the length of the quadriceps induced large variability in Pialk quantification (CV: 21.1%). In contrast, measurement variability due to repeated shims on consecutive scans from the same muscle sample (CV: 6.6%), and the automated spectral processing procedure, were minor (CV: 2.3%). Both metrics of absolute and relative reproducibility of Pialk were of similar magnitude to other well-resolved metabolites (e.g., phosphocreatine, Pi, and phosphodiester). CONCLUSION: Using multiple metrics, the present study established the high reproducibility of Pialk quantification by 31P-MRS using a surface coil on the quadriceps muscle at 3T. However, large variability in Pialk quantification can originate from positioning the coil on the most distal part of the quadriceps, which should be avoided due to shimming inhomogeneity.

Die Broschüre bietet einen kompakten Überblick über die sächsische Wissenschaftslandschaft mit ihren Hochschulen und Forschungseinrichtungen. Redaktionsschluss: 01.10.2023 / The brochure provides a compact overview of the Saxon research landscape with its universities and research institutions. Redaktionsschluss: 01.10.2023

Empresas de manufatura tradicionalmente concentram os seus esforços em concepção, desenvolvimento, fabricação e comercialização de produtos físicos. No entanto, alguns fabricantes têm alterado suas estratégias de negócio, complementando a venda de produtos com o fornecimento de serviços, incorporando conhecimentos e atividades associados ao seu uso, e gerando maior percepção de valor por parte dos seus clientes. Com isso, a competição estratégica por meio de serviços tem se tornado uma marca distintiva das empresas de manufatura inovadoras, proporcionando à empresa um meio robusto para proteger o seu mercado dos concorrentes. Assim, observa-se um interesse crescente em pesquisas sobre o papel dos serviços para sustentar a competitividade da indústria. Abordagens orientadas a serviço, que incorporam esta mudança de foco do negócio, da oferta de produtos isolados para soluções integradas, têm sido tratadas na comunidade acadêmica como servitização, e tipicamente acarretam inovações no modelo de negócios (MN) da empresa. O objetivo deste trabalho é investigar como as empresas podem promover inovações em seus MNs para suportar suas estratégias de servitização, e propor um conjunto de artefatos para apoiá-las na implantação destas estratégias, a partir do seguinte problema de pesquisa: \"Como inovar o MN de uma empresa que adota a estratégia de servitização?\", que se desdobra nas seguintes questões: (i) quais são os principais fatores motivadores para uma empresa adotar a estratégia de servitização?; (ii) como representar o MN de uma empresa incorporando a lógica dominante de serviço, mais adequada para servitização?; (iii) como inovar o MN de uma empresa para implantar sua estratégia de servitização?; e (iv) como aplicar os artefatos propostos para apoiar a implantação da estratégia de servitização em uma empresa? Realiza-se uma pesquisa de natureza exploratória e prescritiva, baseada no método design scienceresearche amparada por uma revisão sistemática da literatura sobre os temas correlatos, visando propor artefatos em resposta às questões colocadas. A demonstração de um dos artefatos propostos é feita com suporte de um estudo de caso em uma empresa multinacional, fabricante de equipamentos médicos, seguida por uma avaliação empírica desta demonstração, suportada pelo método thinkingaloud. A partir das quatro questões de pesquisa, são obtidos os seguintes resultados: (i) identificação, análise e categorização dos principais fatores motivadores para adoção da servitização; (ii) proposição de uma arquitetura de MN incorporando a lógica dominante de serviço, para facilitar o estudo da servitização; (iii) proposição de um processo de inovação do MN da empresa, para implantar a servitização. e (iv) demonstração e avaliação da arquitetura proposta de MN para servitização. A pesquisa oferece contribuições à literatura de MN e servitização, e aos gestores de empresas, propondo os artefatos canvas do modelo de negócios para servitização (CMNS) e o processo IPIDI para inovação do MN para servitização, além de contribuições metodológicas relativas a design scienceresearch e thinking aloud. / Manufacturing companies traditionally are focused on designing, developing, manufacturing and marketing physical products. However, some manufacturers are changing their business strategies, complementing the sale of products by providing services, adding knowledge and activities associated with their use, and creating a higher perception of value by their customers.Strategic competition through service delivery has become a hallmark of the innovative manufacturing companies, providing the companies with a robust means to protect their market from competitors. Thus, there is a growing interest in researching the role of services in sustaining the competitiveness of manufacturing industry.Service-oriented approaches, which incorporate this shift in business\' focus from offering isolated products, to proposing integrated solutions, have been addressed in the literature as servitization, and typically entail innovations in the company\'s business model (BM). The goal of this research is investigating how companies can promote transformations in their BMs to support their servitization strategies, and proposing a set of artifacts to support them in the implementation of these strategies, since the following research problem: \"How to innovate the BM of a company which adopts the servitization strategy? \", which unfolds in the following questions: (i) what are the main motivating factors for a company to adopt the servitization strategy?; (ii) how to represent the BM of a company incorporating the service-dominant logic, more suitable for servitization?; (iii) how to innovate a company\'s BM to implement its servitization strategy ?; and (iv) how to apply the proposed artifacts to support the implementation of the servitization strategy in a company? An exploratory and prescriptive research is carried out, based on the design science research methodology, and supported by a systematic literature review on the related subjects, aiming at proposing artifacts in response to the questions posed. The demonstration of the proposed artifacts is done by means of a case study in a multinational company, which manufactures medical devices, followed by an assessment of this demonstration, supported by the thinking aloud method. From the four research questions, the following results are obtained: (i) identification, analysis and categorization of the main motivating factors for servitization adoption; (ii) proposing a BM architecture according to the service-dominant logic, to help the servitization study; (iii) proposing an innovation process for the company\'s BM, to implement the servitization; and (iv) demonstration and assessment of the proposed BM architecture for servitization. The research offers contributions to the literature of BM and servitization, and to practitioners, proposing the artifacts business model canvas for servitization (BMCS) and the IPIDI process for innovation of BM for servitization, besides some methodological contributions related to design science research and thinking aloud.

Muitos são os potenciais benefícios e obstáculos envolvendo a implantação de iniciativas de dados governamentais abertos (DGA). Dentro do universo de dados passíveis de serem abertos, os Diários Oficiais (DOs) constituem um exemplo ainda pouco estudado. Tendo em vista que os atos públicos se tornam válidos através da publicação em DOs, fica evidente a importância das informações contidas nesses documentos. Essas informações podem vir então a ser melhor reutilizadas uma vez que sejam publicadas seguindo os preceitos de DGA. Este trabalho teve como objetivos desenvolver uma ferramenta para a publicação de um DO em formato aberto, incluindo diversos grupos na definição de seus requisitos e documentando todo o processo. Utilizando uma metodologia inspirada na Design Science Research e na Pesquisa-Ação, foi firmada uma parceria com um órgão público, atuando no processo de abertura dos dados. O processo buscou permitir algum nível de participação através de eventos e questionários. O levantamento de requisitos considerou as necessidades dos gestores envolvidos, a literatura relacionada e as colocações feitas por participantes dos eventos e questionários, buscando assim uma aferição mais completa das necessidades relacionadas. O acompanhar do processo também permitiu algumas considerações sobre os interesses e desafios envolvidos no mesmo. A ferramenta desenvolvida tem recebido um número crescente de acessos e boas avaliações por parte de usuários tanto internos como externos à administração pública. Algumas tentativas de reúso automático dos dados publicados já vêm ocorrendo, mostrando os desafios e potencialidades dos mesmos / Many are the potential benefits and obstacles related to open government data (OGD) initiatives. Among all the data that could be opened, the Official Gazettes (OGs) are one example still demanding better studies. Noting that the public acts only become valid through publication in OGs, it is evident the importance of the information within these documents. This information can then be better reused if published following OGD principles. This work, adopting a methodology inspired by action research, and in partnership with a public body, acted in an OGD initiative and in the development of a tool complying with the requirements of its publication. The process sought to allow some level of participation through public events and questionnaires. The requirement analysis took into consideration the demands of the involved civil servants, related literature and the opinions of the participants of the events and questionnaires, seeking a more complete view about the related demands. Throughout the entire process the challenges and interests were explored, pursuing a better understanding of matter. The tool has received an increasing number of accesses and good reviews by users from the public administration and in general. Some attempts were made to automatically reuse the published data, demonstrating the potentialities and challenges of them