Field pea is an important crop in western Canada. The current recommended seeding rate in field pea is 88 plants m-2. As certain pea genotypes have the ability for increased branching, it may be possible for a producer to seed at a lower plant population without reduced yield or to choose a highly branched cultivar to have reduced risk of yield loss under conditions of poor emergence. The objective of this research was to determine how differences in branching among seven representative pea cultivars affected crop yield at different seeding rates, and to determine if branching affected the competitive ability of pea cultivars. In the plant population experiment, seven pea cultivars were seeded at five target plant populations (10, 30, 90, 120, and 150 plant m-2) during 2005 and 2006 at Rosthern and Saskatoon, Saskatchewan. The competition experiment involved eight cultivars being seeded at 50 plants m-2 to measure competitiveness with weeds. Plant emergence, number of branches, light interception, harvest index and grain yield were measured. Growth, seasonal temperature and rainfall were near normal in 2005. Severe terminal drought occurred in 2006 which may have lead to decreased yields. Branching was greatest at low plant densities and decreased as plant density increased. Grain yield increased as plant density increased until it plateaued at 80 100 plants m-2. The response of yield to plant density differed to some extent among cultivars, with CDC Acer and CDC Bronco achieving more of their potential yield at lower densities, while Carrera and Courier required higher densities to reach the same proportion of potential yield. Weed biomass was lowest in plots sown to longer vined cultivars with normal leaf type. Branching habit did not affect the competitiveness of pea cultivars. Potential exists to plant highly branched cultivars to reduce risk of yield loss in situations where low plant emergence might occur.

There is a demand for new recommendations for pulse seeding rates that will meet the needs of organic farmers. This study was conducted to determine the impact of seeding rate on N2 fixation and N accumulation in lentil and pea and to examine the impact of different seeding rates of lentil and pea on the productivity and N-uptake (i.e., N benefit) in a subsequent wheat crop.<p> The study was performed between 2005 and 2007. Two sites were selected each year of the two-year experiment on certified organic farms in central Saskatchewan. At each location, lentil (<i>Lens culinaris</i> L.) cultivar CDC Sovereign and field pea (<i>Pisum sativum</i> L.) cultivar CDC Mozart were each seeded at five different rates. Wheat (<i>Triticum aestivum</i> L.) cultivar AC Elsa was sown as a non-fixing reference crop at a plant population density of 250 seeds m-2. In the following year, wheat was sown to assess the effect of the pulse seeding rate treatments on the succeeding crop.<p> The pulse crop seeding rates significantly affected the quantity of N2 fixed of lentil and field pea, although %Ndfa (80 to 88% and 79 to 85% for lentil and pea, respectively) typically was unaffected by seeding rate. Yield parameters of following wheat crop were not affected by the seeding rates of the previous pulses. Typically, N contributions increased with increasing seeding rates of both lentil and pea, but there was no detectable difference in N uptake by the following wheat grown on the both pulse stubble. The different seeding rates of organically grown lentil and field pea have impacts on the amount of N2 fixed and N contribution to the soil. However, the differences in N remaining in the soil at different seeding rates of the pulse crops were not detectable in the following wheat crop and the soil N in the following year.

The inability to control wild oat (Avena fatua L.) in oat (Avena sativa L.) crops by chemical means limits growers to the use of cultural control methods. Delayed seeding is the most commonly used measure; however, both crop yield and quality may suffer as a result. The objectives of this research were to i) determine if western Canadian oat genotypes differ in competitive ability, ii) determine the effect of increased plant populations on oat wild oat competition, iii) determine the effect of wild oat competition on oat quality, and iv) establish whether or not oat genotype and seed size affect germination characteristics under low temperature and moisture stress. These objectives were tested using field and laboratory experiments. Morphologically diverse oat genotypes differed in their ability to both tolerate wild oat competition and interfere with wild oat growth. Although low yielding under weed-free conditions, when subject to wild oat competition CDC Bell was able to maintain yield, reduce wild oat seed production and was the most competitive of the genotypes examined. Increased plant populations achieved through higher seeding rates provide an effective means by which to enhance the competitive ability of oat genotypes resulting in reduced yield loss and wild oat seed production. With the exception of the percentage of wild oat seed in harvested oat samples, wild oat competition had minimal effect on oat quality. Differences in germination characteristics were observed among the genotypes examined. Conclusions that emerge from this research are that i) oat genotypes differ in their ability to tolerate and interfere with wild oat competition, ii) increased plant populations may provide a long-term control measure that may reduce weed seed contribution to the soil seedbank as well as enhance the competitive ability of oat, iii) wild oat competition has minimal effect on milling oat quality with the exception of percentage of wild oat seed in harvested samples and iv) that median germination time varies among oat genotypes.

The inability to control wild oat (Avena fatua L.) in oat (Avena sativa L.) crops by chemical means limits growers to the use of cultural control methods. Delayed seeding is the most commonly used measure; however, both crop yield and quality may suffer as a result. The objectives of this research were to i) determine if western Canadian oat genotypes differ in competitive ability, ii) determine the effect of increased plant populations on oat wild oat competition, iii) determine the effect of wild oat competition on oat quality, and iv) establish whether or not oat genotype and seed size affect germination characteristics under low temperature and moisture stress. These objectives were tested using field and laboratory experiments. Morphologically diverse oat genotypes differed in their ability to both tolerate wild oat competition and interfere with wild oat growth. Although low yielding under weed-free conditions, when subject to wild oat competition CDC Bell was able to maintain yield, reduce wild oat seed production and was the most competitive of the genotypes examined. Increased plant populations achieved through higher seeding rates provide an effective means by which to enhance the competitive ability of oat genotypes resulting in reduced yield loss and wild oat seed production. With the exception of the percentage of wild oat seed in harvested oat samples, wild oat competition had minimal effect on oat quality. Differences in germination characteristics were observed among the genotypes examined. Conclusions that emerge from this research are that i) oat genotypes differ in their ability to tolerate and interfere with wild oat competition, ii) increased plant populations may provide a long-term control measure that may reduce weed seed contribution to the soil seedbank as well as enhance the competitive ability of oat, iii) wild oat competition has minimal effect on milling oat quality with the exception of percentage of wild oat seed in harvested samples and iv) that median germination time varies among oat genotypes.

Field pea is an important crop in western Canada. The current recommended seeding rate in field pea is 88 plants m-2. As certain pea genotypes have the ability for increased branching, it may be possible for a producer to seed at a lower plant population without reduced yield or to choose a highly branched cultivar to have reduced risk of yield loss under conditions of poor emergence. The objective of this research was to determine how differences in branching among seven representative pea cultivars affected crop yield at different seeding rates, and to determine if branching affected the competitive ability of pea cultivars. In the plant population experiment, seven pea cultivars were seeded at five target plant populations (10, 30, 90, 120, and 150 plant m-2) during 2005 and 2006 at Rosthern and Saskatoon, Saskatchewan. The competition experiment involved eight cultivars being seeded at 50 plants m-2 to measure competitiveness with weeds. Plant emergence, number of branches, light interception, harvest index and grain yield were measured. Growth, seasonal temperature and rainfall were near normal in 2005. Severe terminal drought occurred in 2006 which may have lead to decreased yields. Branching was greatest at low plant densities and decreased as plant density increased. Grain yield increased as plant density increased until it plateaued at 80 100 plants m-2. The response of yield to plant density differed to some extent among cultivars, with CDC Acer and CDC Bronco achieving more of their potential yield at lower densities, while Carrera and Courier required higher densities to reach the same proportion of potential yield. Weed biomass was lowest in plots sown to longer vined cultivars with normal leaf type. Branching habit did not affect the competitiveness of pea cultivars. Potential exists to plant highly branched cultivars to reduce risk of yield loss in situations where low plant emergence might occur.

There is a demand for new recommendations for pulse seeding rates that will meet the needs of organic farmers. This study was conducted to determine the impact of seeding rate on N2 fixation and N accumulation in lentil and pea and to examine the impact of different seeding rates of lentil and pea on the productivity and N-uptake (i.e., N benefit) in a subsequent wheat crop.<p> The study was performed between 2005 and 2007. Two sites were selected each year of the two-year experiment on certified organic farms in central Saskatchewan. At each location, lentil (<i>Lens culinaris</i> L.) cultivar CDC Sovereign and field pea (<i>Pisum sativum</i> L.) cultivar CDC Mozart were each seeded at five different rates. Wheat (<i>Triticum aestivum</i> L.) cultivar AC Elsa was sown as a non-fixing reference crop at a plant population density of 250 seeds m-2. In the following year, wheat was sown to assess the effect of the pulse seeding rate treatments on the succeeding crop.<p> The pulse crop seeding rates significantly affected the quantity of N2 fixed of lentil and field pea, although %Ndfa (80 to 88% and 79 to 85% for lentil and pea, respectively) typically was unaffected by seeding rate. Yield parameters of following wheat crop were not affected by the seeding rates of the previous pulses. Typically, N contributions increased with increasing seeding rates of both lentil and pea, but there was no detectable difference in N uptake by the following wheat grown on the both pulse stubble. The different seeding rates of organically grown lentil and field pea have impacts on the amount of N2 fixed and N contribution to the soil. However, the differences in N remaining in the soil at different seeding rates of the pulse crops were not detectable in the following wheat crop and the soil N in the following year.

The importance of shear stress in vascular biology and pathophysiology has been highlighted by the focal development patterns of atherosclerosis, abdominal aortic aneurysms, and heart valve disease in regions exposed to disturbed flow leading to low or oscillatory shear stress at the wall of the blood vessel or the surface of the valve leaflet. The novel and significant finding of this study is that mouse aortic endothelial cell exposure to pro-atherogenic oscillatory shear stress (OS) (+/- 5 dynes/cm2) increased their production of cathepsins, the family of lysosomal cysteine proteases that are potent elastases and collagenases leading to protease degradation and remodeling of the extracellular matrix structural components. Conversely, atheroprotective unidirectional laminar shear stress (LS) (15 dynes/cm2) decreased elastase and gelatinase activities of endothelial cells through a shear stress mediated reduction in cathepsins K, L, and S activity. Their endogenous inhibitor, cystatin C, was found to be inversely regulated by shear stress; LS increased its secretion by endothelial cells while OS decreased it. Binding of free cystatin C in the conditioned media to carboxymethylated papain coated agarose beads led to an increase in cathepsin activity since the available cathepsin was not inhibited. To verify these findings in human samples, immunohistochemical analysis of cystatin C and cathepsin K was performed on human coronary arteries. Cathepsin K stained strongly in the endothelial layer of vessels with degraded internal elastic lamina while cystatin C staining intensity was strongest overlying minimally diseased vessels. Additional roles for cathepsins K, L, and S were found in endothelial cell alignment in response to unidirectional laminar shear stress, endothelial cell migration, and programmed cell death. We conclude that there is an inverse regulation of cathepsins and cystatin C in endothelial cells by LS and OS and identify the cathepsin family of proteases as potential targets for therapeutic intervention of cardiovascular disease development at sites of disturbed flow.

BACKGROUND: An important characteristic of transitional cell carcinoma (TCC) is the formation of tumors in multiple sites throughout the whole urinary tracts. Two theories explain the pathophysiologic mechanisms of multifocal tumors: (1) intraluminal seeding, it indicates the multiple tumors come from a single transformed malignant cell with secondary seeding or migration at different sites, and (2) the field cancerization, carcinogens affect the urothelium at multiple sites, leading to numerous mutation and independent growth of multifocal polyclonal tumors. Multifocal urothelial carcinomas could come from intraluminal seeding or from field cancerization. However, the data of clinical behaviors between the two different tumor types are lacking. METHODS: Bilateral synchronous and metachronous primary TCC of the upper urinary tracts were derived from field cancerization. Recurrent bladder cancers following upper-tract tumors mostly come from intraluminal seeding. The recurrence, progression, and prognosis of the two different tumors were analyzed. RESULTS: Bilateral upper-tract urothelial carcinomas derived from field cancerization were frequently associated with renal insufficiency, which were more invasive and had poor prognosis than bladder tumors derived from intraluminal seeding. CONCLUSION: The clinical behaviors of the multiple urothelial tumors derived from field cancerization and from intraluminal seeding should be different.

Den här studien är en uppföljning av föryngringsresultatet efter maskinell sådd av contorta ( Pinus contorta Dougl. var. latifolia Engelm.) i Härjedalen. Efter tre växtsäsonger uppgick det totala antalet såddplantor till 3 700 per ha och antalet huvudplantor uppgick till 1 800 per ha. Andelen nollytor uppgick till 2 %. Det var ingen skillnad i föryngringsresultat mellan låg och hög altitud. Generellt gav maskinell sådd av contorta ett tillfredsställande föryngringsresultat. På mindre bördig mark var såväl det totala antalet såddplantor som antalet huvudplantor högre än på bördigare mark. / Mechanical regeneration by seeding of lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) in the province of Härjedalen in Sweden was studied. After three growing seasons the total number of seedlings were 3 700 per ha and the number of main seedlings were 1 800 per ha. 2% of the sample plots had no plants within 3 m. No difference could be found between objects on high and low altitudes. In general mechanical seeding gave satisfactory regeneration. On low fertile soils the total number of seedlings as well as the future main seedlings was higher than on more fertile soils.

The misfolding and aggregation of the beta cell hormone islet amyloid polypeptide (IAPP) into amyloid fibrils is the main pathological finding in islets of Langerhans in type 2 diabetes. Pathological assemblies of IAPP are cytotoxic and believed to contribute to the loss of insulin-producing beta cells. Changes in the microenvironment that could trigger the aggregation of IAPP are largely unknown. So is the possibility that islet amyloid can spread within or between tissues. The present thesis have explored the roles of glycosaminoglycan heparan sulfate (HS) and the novel anti-amyloid chaperone Bri2 BRICHOS domain in the assembly of IAPP amyloid and cytotoxic IAPP aggregates. Furthermore, cross-seeding as a molecular interaction between the observed connection of type 2 diabetes and Alzheimer disease has been examined. The N-terminal region of IAPP was required for binding to HS structures and induction of binding promoted amyloid formation. Interference in the HS-IAPP interaction by heparanase degradation of HS or by introducing short, soluble HS-structure fragments reduced amyloid deposition in cultured islets. Cytotoxicity induced by extracellular, aggregating IAPP was mediated via interactions with cell-surface HS. This suggests that HS plays an important role in islet amyloid deposition and associated toxicity. BRICHOS domain containing protein Bri2 was highly expressed in human beta cells and colocalized with IAPP intracellularly and in islet amyloid deposits. The BRICHOS domain effectively attenuated both IAPP amyloid formation and IAPP-induced cytotoxicity. These results propose Bri2 BRICHOS as a novel chaperone preventing IAPP aggregation in beta cells. The intravenous injection of IAPP, proIAPP or amyloid-β (Aβ) fibrils enhanced islet amyloidosis in transgenic human IAPP mice, demonstrating that both homologous- and heterologous seeding of islet amyloid can occur in vivo. IAPP colocalized with Aβ in brain amyloid from AD patients, and AD patients diagnosed with T2D displayed increased proportions of neuritic plaques, the more pathogenic plaque subtype. In conclusion, both IAPP amyloid formation and the cytotoxic effects of IAPP is dependent on interactions with HS whereas interactions with Bri2 BRICHOS is protective. Cross-seeding between Aβ and IAPP can occur in vivo and the two peptides colocalize in brain amyloid in AD patients.