<p>This thesis describes results from fundamental studies of liposomes intended for drug delivery and pH or temperature triggered release. In addition, the effect of lipid composition on bilayer disc formation and a potential application of the bilayer discs were investigated.</p><p>The lower pH encountered by endocytosed liposomes can be utilized to trigger drug release. The mechanisms behind cytosolic drug delivery were investigated using two different kinds of pH-sensitive liposomes. The results indicate that incorporation of non-lamellar forming lipids into the endosome membrane may allow for drug escape into the cytosol.</p><p>Temperature-sensitive liposomes containing lysolipid (LTSL) release their content almost instantly when heated to temperatures close to the gel to liquid crystalline phase transition temperature (T<i>C</i>). Morphological changes of the liposomes in response to temperature cycling were studied. Temperature cycling induced liposome openings and disintegration of the liposomes into bilayer discs. Incubation of LTSL in the presence of multilamellar liposomes (MLVs) resulted in relocalisation of lysolipid into the MLVs, which affected the rapid release from LTSL. We propose that the presence of micelle-forming components, such as lysolipids and PEG-lipids, facilitates the formation of defects and membrane openings during the initial phase of membrane melting, resulting in the observed rapid release. Similar to added lysolipids, also hydrolysis generated lysolipids induce disc-formation upon heating through T<i>C</i> of the lipid mixture.</p><p>Two fundamentally different micelles may form in PEG-lipid/lipid mixtures. We found that discoidal structures are preferred over cylindrical micelles when the mixture contains components that reduce the spontaneous curvature, increase the monolayer bending modulus, or reduce PEG-lipid/lipid miscibility. The large discoidal micelles found at low PEG-lipid content are better described as bilayer discs. We evaluated such discs as model membranes in drug partitioning studies, and suggest that they, in some cases, produce more accurate data than liposomes.</p>

This thesis describes results from fundamental studies of liposomes intended for drug delivery and pH or temperature triggered release. In addition, the effect of lipid composition on bilayer disc formation and a potential application of the bilayer discs were investigated. The lower pH encountered by endocytosed liposomes can be utilized to trigger drug release. The mechanisms behind cytosolic drug delivery were investigated using two different kinds of pH-sensitive liposomes. The results indicate that incorporation of non-lamellar forming lipids into the endosome membrane may allow for drug escape into the cytosol. Temperature-sensitive liposomes containing lysolipid (LTSL) release their content almost instantly when heated to temperatures close to the gel to liquid crystalline phase transition temperature (TC). Morphological changes of the liposomes in response to temperature cycling were studied. Temperature cycling induced liposome openings and disintegration of the liposomes into bilayer discs. Incubation of LTSL in the presence of multilamellar liposomes (MLVs) resulted in relocalisation of lysolipid into the MLVs, which affected the rapid release from LTSL. We propose that the presence of micelle-forming components, such as lysolipids and PEG-lipids, facilitates the formation of defects and membrane openings during the initial phase of membrane melting, resulting in the observed rapid release. Similar to added lysolipids, also hydrolysis generated lysolipids induce disc-formation upon heating through TC of the lipid mixture. Two fundamentally different micelles may form in PEG-lipid/lipid mixtures. We found that discoidal structures are preferred over cylindrical micelles when the mixture contains components that reduce the spontaneous curvature, increase the monolayer bending modulus, or reduce PEG-lipid/lipid miscibility. The large discoidal micelles found at low PEG-lipid content are better described as bilayer discs. We evaluated such discs as model membranes in drug partitioning studies, and suggest that they, in some cases, produce more accurate data than liposomes.

The Neoproterozoic was a time of major change in Earth’s surficial history, including a major rise in atmospheric oxygen, the first appearance of complex metazoan life, and a series of worldwide glaciations. A particular interesting element of these so called “snowball Earth” glacial deposits is the presence of iron formation, a distinctive Precambrian rock type that is largely absent from the post-Paleoproterozoic record. Despite being relatively poorly studied with respect to their geochemistry and sedimentology, Neoproterozoic iron formations are used to support many models for the record of oxygen concentrations of the Earth. The classical example of Neoproterozoic iron formation is the Rapitan iron formation of northwestern Canada. This hematite-jasper iron formation is associated with glaciogenic turbidites and diamictites. Despite being the archetype, the Rapitan iron formation has not been studied in the context of recent ideas about the Neoproterozoic. In this thesis, the stratigraphy, geochemistry, and basin architecture of the Rapitan iron formation are reassessed. Using the REE+Y and the redox-sensitive elements Mo and U, it is shown that the Rapitan iron formation was deposited in a partially restricted basin from biogenically reduced iron under variable redox conditions. Elemental Re and Mo isotopes further show that although oxic and ferruginous conditions predominated during deposition of the iron formation, a transition towards a sulfidic water column locally terminated deposition. Finally, regional stratigraphy and geochemistry show that the iron formation was preferentially deposited in deep, newly formed basins that were protected from significant siliciclastic sedimentation. These basins were delimited by inferred crustal-scale faults trending roughly perpendicular to the axis of the rift basin, and allowed significant changes in thickness and sedimentological character over short distances along strike. These factors help build an overall geotectonic regime under which Neoproterozoic iron formations were deposited: young, deep rift basins that had undergone marine incursion, and were intermittently sealed by an ice shelf, allowing for the generation of an anoxic, iron-rich water column. The absence of the Eu anomaly and the heavy Mo isotopic signature indicate that the open ocean was fully oxygenated at the time of Rapitan iron formation deposition, as opposed to ferruginous as previously suggested.

Previous studies have shown that ethically loaded design sit- uations within design currently present themselves as an implicit and non-reflexive activity. Others promote a development of ethi- cal tools which are incorporated within the normal set of methods and tools used during the design process. Within the service de- sign discipline no such research has been identified. In order to shed a light on the ethics within service design this thesis explores the ethical design ecology of service design and gives a first sketch of an ethical baseline for the field. The data collected in the study represents five weeks of shadowing in-house and external service design consultants working in Scandinavia. The data was analyzed by means of the three major normative theories within ethics and the Value-Sensitive Design framework. The analysis tools were ap- plied through a three step process where situations first were iden- tified, then the value-sensitive situations were flagged by means of the VSD-framework. Finally these value-sensitive situations were looked at from an ethical perspective using the three major ethical normative theories, consequentialism, deontology and virtue ethics. The results demonstrate that service designers often approach eth- ical problems in an implicit and ethically consequentialist way and that when ethical situations are dealt with explicitly they are often of a nature in which the consequences of the proposed design solution easily can be foreseen.

Research tools to identify sensitive topics and thus new opportunities to design for have grown in popularity in the last twenty years within HCI, with many projects and areas to note. However, the research tools used are still underdeveloped (Crabtree, 2003), and many universal designs of the 20th century have failed to develop for more sensitive areas, bar the conventional young, non-disabled, white, cis-male (Clarkson, 2003). The topics discussed in the thesis are reviews and arguments for the use of an adapted cultural probe’s place to research sensitive topics, identify perhaps previously hidden “sensitive-HCI” (Waycott et al. 2015) design spaces. The focus is on the tools to gather data, and discover design opportunities, rather than the particular and actual findings from the study.

An innovative approach for characterization of one dimensional Photonic Band Gap structures comprised of metallic inclusions (i.e. subwavelength dipole elements or resonant ring elements) is presented. Through an efficient S- to T-parameters conversion technique, a detailed analysis has been performed to investigate the variation of the dispersion characteristics of 1-D PBG structures as a function of the cell element configuration. Also, for the first time, the angular sensitivity of the structure has been studied in order to obtain the projected band diagrams for both TE and TM polarizations. Polarization sensitivity of the subwavelength cell element is exploited to propose a novel combination of elements which allows achieving PBGs with simultaneous frequency and polarization selectivity. The proposed approach demonstrates that the dispersion characteristic of each orthogonal polarization can be independently adjusted with dipole elements parallel to that same polarization. Generally, the structure has potential applications in orthomode transducer, and generally whenever the polarization of the incoming signal is to be used as a means of separating it from another signal in the same frequency band that is of orthogonal polarization. The current distribution and the resonance behavior of the ring element is studied and the effect of resonance on dispersion characteristics of 1-D PBGs composed of rings is investigated for the first time, for both individual and coupled rings. Interestingly, it is observed that 1-D PBG composed of resonant elements consistently has a bandgap around the resonant frequency of the single layer structure.

Rendre les données multimédias disponibles en ligne devient moins cher et plus pratique sur une base quotidienne, par exemple par les utilisateurs eux-mêmes. Des phénomènes du Web comme Facebook, Twitter et Flickr bénéficient de cette évolution. Ces phénomènes et leur acceptation accrue conduisent à une multiplication du nombre d’images disponibles en ligne. La taille cumulée de ces images souvent publiques et donc consultables, est de l’ordre de plusieurs zettaoctets. L’exécution d’une requête de similarité sur de tels volumes est un défi que la communauté scientifique commence à cibler. Une approche envisagée pour faire face à ce problème propose d’utiliser un système distribué et hétérogène de recherche d’images basé sur leur contenu (CBIRs). De nombreux problèmes émergent d’un tel scénario. Un exemple est l’utilisation de formats de métadonnées distincts pour décrire le contenu des images; un autre exemple est l’information technique et structurelle inégale. Les métriques individuelles qui sont utilisées par les CBIRs pour calculer la similarité entre les images constituent un autre exemple. Le calcul de bons résultats dans ce contexte s’avère ainsi une tàche très laborieuse qui n’est pas encore scientifiquement résolue. Le problème principalement abordé dans cette thèse est la recherche de photos de CBIRs similaires à une image donnée comme réponse à une requête multimédia distribuée. La contribution principale de cette thèse est la construction d’un réseau de CBIRs sensible à la sémantique des contenus (CBIRn). Ce CBIRn sémantique est capable de collecter et fusionner les résultats issus de sources externes spécialisées. Afin d’être en mesure d’intégrer de telles sources extérieures, prêtes à rejoindre le réseau, mais pas à divulguer leur configuration, un algorithme a été développé capable d’estimer la configuration d’un CBIRS. En classant les CBIRs et en analysant les requêtes entrantes, les requêtes d’image sont exclusivement transmises aux CBIRs les plus appropriés. De cette fac ̧on, les images sans intérêt pour l’utilisateur peuvent être omises à l’avance. Les images retournées cells sont considérées comme similaires par rapport à l’image donnée pour la requête. La faisabilité de l’approche et l’amélioration obtenue par le processus de recherche sont démontrées par un développement prototypique et son évaluation utilisant des images d’ImageNet. Le nombre d’images pertinentes renvoyées par l’approche de cette thèse en réponse à une requête image est supérieur d’un facteur 4.75 par rapport au résultat obtenu par un réseau de CBIRs predéfini. / Making multimedia data available online becomes less expensive and more convenient on a daily basis. This development promotes web phenomenons such as Facebook, Twitter, and Flickr. These phenomena and their increased acceptance in society in turn leads to a multiplication of the amount of available images online. This vast amount of, frequently public and therefore searchable, images already exceeds the zettabyte bound. Executing a similarity search on the magnitude of images that are publicly available and receiving a top quality result is a challenge that the scientific community has recently attempted to rise to. One approach to cope with this problem assumes the use of distributed heterogeneous Content Based Image Retrieval system (CBIRs). Following from this anticipation, the problems that emerge from a distributed query scenario must be dealt with. For example the involved CBIRs’ usage of distinct metadata formats for describing their content, as well as their unequal technical and structural information. An addition issue is the individual metrics that are used by the CBIRs to calculate the similarity between pictures, as well as their specific way of being combined. Overall, receiving good results in this environment is a very labor intensive task which has been scientifically but not yet comprehensively explored. The problem primarily addressed in this work is the collection of pictures from CBIRs, that are similar to a given picture, as a response to a distributed multimedia query. The main contribution of this thesis is the construction of a network of Content Based Image Retrieval systems that are able to extract and exploit the information about an input image’s semantic concept. This so called semantic CBIRn is mainly composed of CBIRs that are configured by the semantic CBIRn itself. Complementarily, there is a possibility that allows the integration of specialized external sources. The semantic CBIRn is able to collect and merge results of all of these attached CBIRs. In order to be able to integrate external sources that are willing to join the network, but are not willing to disclose their configuration, an algorithm was developed that approximates these configurations. By categorizing existing as well as external CBIRs and analyzing incoming queries, image queries are exclusively forwarded to the most suitable CBIRs. In this way, images that are not of any use for the user can be omitted beforehand. The hereafter returned images are rendered comparable in order to be able to merge them to one single result list of images, that are similar to the input image. The feasibility of the approach and the hereby obtained improvement of the search process is demonstrated by a prototypical implementation. Using this prototypical implementation an augmentation of the number of returned images that are of the same semantic concept as the input images is achieved by a factor of 4.75 with respect to a predefined non-semantic CBIRn.

Beaucoup d’applications en ingénierie demandent l’utilisation de matériaux intelligents qui peuvent se déformer en réponse à un stimulus extérieur. C’est dans ce contexte, que s’est posé ce projet de recherche. Bénéficiant d’un environnement pluridisciplinaire, grâce à l’association de deux axes de l'Institut Pascal : l’axe MMS (Mécanique, Matériaux et Structures) et l’axe PHOTON (Axe Photonique, Ondes, Nanomatériaux), cette thèse s’intègre parfaitement dans l’action transversale "Matériaux et Modélisations multi-échelles" du laboratoire. La première partie de ce travail s'appuie sur un système expérimental mis au point par une équipe américaine [Chang_10] qui permet la mesure sans contact du pH d'une solution en exploitant les caractéristiques photoniques du système. Ce système est composé d'un réseau d'hydrogel fixé sur un substrat rigide. Un modèle numérique est développé dans le but de simuler le fonctionnement de l'ensemble et d'optimiser le réseau d'hydrogel en vue d'applications dans le domaine médical. La seconde partie de ce travail concerne le développement d'une théorie sur le comportement mécanique de polymères sensibles à la lumière. L'objectif est d'établir une relation liant la déformation du matériau à l’intensité lumineuse. Les résultats obtenus sont comparés avec les résultats expérimentaux issus de la littérature. L'influence des interactions entre les molécules d'azobenzènes sur la déformation du matériau est étudiée. / Many engineering applications involve stimuli-responsive materials that can change their shape under the action of an external stimulus. It is in this context that this project takes place. Thanks to a multidisciplinary environment with the association of two lines of research of the Institut Pascal: the Mechanical area (Mechanic, Materials and structure) and the Photonic area (Nanostructures and Nanophotonics), this PhD perfectly fits with the “Materials and multi-scale Modeling” transversal action of the laboratory. The first part of this work relies on an experimental system developed by an American team [Chang_10] which allows to measure the pH of a solution without contact, making use of its photonic characteristics. This system is composed of a hydrogel network fixed on a rigid substract. A numerical model is developed in order to simulate its behavior and optimize the hydrogel network with a view to applications in the medical domain. The second part of this PhD is related to the development of a theory on the mechanical behavior of photo-sensitive polymers. The aim is to establish a link between the material deformation and the light intensity. The obtained results are compared to experimental ones from literature. The interaction influence of the azobenzenes molecules on the material strain is studied.

An innovative approach for characterization of one dimensional Photonic Band Gap structures comprised of metallic inclusions (i.e. subwavelength dipole elements or resonant ring elements) is presented. Through an efficient S- to T-parameters conversion technique, a detailed analysis has been performed to investigate the variation of the dispersion characteristics of 1-D PBG structures as a function of the cell element configuration. Also, for the first time, the angular sensitivity of the structure has been studied in order to obtain the projected band diagrams for both TE and TM polarizations. Polarization sensitivity of the subwavelength cell element is exploited to propose a novel combination of elements which allows achieving PBGs with simultaneous frequency and polarization selectivity. The proposed approach demonstrates that the dispersion characteristic of each orthogonal polarization can be independently adjusted with dipole elements parallel to that same polarization. Generally, the structure has potential applications in orthomode transducer, and generally whenever the polarization of the incoming signal is to be used as a means of separating it from another signal in the same frequency band that is of orthogonal polarization. The current distribution and the resonance behavior of the ring element is studied and the effect of resonance on dispersion characteristics of 1-D PBGs composed of rings is investigated for the first time, for both individual and coupled rings. Interestingly, it is observed that 1-D PBG composed of resonant elements consistently has a bandgap around the resonant frequency of the single layer structure.

Syftet med den här studien är att undersöka vad som skiljer i personlighet mellan studenter som vaccinerade sig mot svininfluensan och studenter som inte vaccinerade sig. Studien genomfördes genom en kvantitativ enkätundersökning på 194 studenter från ett universitet i Svealand. Deltagarna fick besvara psykometriska personlighetstester som mätte känslighet, ångest, depression, sympati och pålitlighet. Därefter utfördes statistiska test i SPSS för att i huvudsak beräkna medelvärdesskillnader. Resultaten visar att vaccinerade studenter är signifikant känsligare än ovaccinerade studenter. Vaccinerade studenter är också mer ångestfyllda men skillnaderna är inte signifikanta. Det finns inga nämnvärda skillnader i depression, sympati eller pålitlighet. Typ av utbildning och kön predicerar heller inte vaccinering. Sammanfattningsvis ska känslighet därmed ses som den viktigaste prediktorn för vilka personer som vaccinerar sig vid pandemier. Resultatet kan generaliseras till den svenska befolkningen eftersom studiens andel vaccinerade motsvarar befolkningens andel vaccinerade. Studiens urval motsvarar däremot inte den svenska populationens genomsnittliga utbildningsnivå. Å andra sidan har utbildningsnivå inte visat sig vara en betydande faktor för hälsoskyddande beteenden i tidigare forskning, varför mitt resultat bör betraktas som betydelsefullt. / The purpose of this study is to examine which personality aspects that are differing from students who got vaccinated against the swine flu, to students who did not get vaccinated. This study carried out a quantitative survey on 194 students from a mid-region university in Sweden. The participants were to answer psychometric personality tests on sensitivity, anxiety, depression, sympathy and dependability. Thereafter, statistical tests were conducted through SPSS, mainly to calculate mean differences. The results conclude that vaccinated students are significantly more sensitive than un-vaccinated students. Vaccinated students are also more anxious but these differences are not significant. There are no notable differences in depression, sympathy or dependability. Type of education and sex do not either predict vaccination. Finally, sensitivity shall be viewed as the most important predictor in a person’s likeliness to get vaccinated at pandemics. The findings can be generalized to the Swedish population because the proportion of vaccinated students in this study corresponds to the proportion of vaccinated people of the Swedish population. However, the sample of this study does not correspond to the educational level of the Swedish population. On the other hand, educational level has not been shown as an important determinant in health protective behaviors in former research literature, why my result should be considered as meaningful.