Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-18T10:52:37Z No. of bitstreams: 1 2015 - Bruna Rodrigues Pereira.pdf: 2671756 bytes, checksum: b38e36947fda465d9d78272a81b2e947 (MD5) / Made available in DSpace on 2017-10-18T10:52:37Z (GMT). No. of bitstreams: 1 2015 - Bruna Rodrigues Pereira.pdf: 2671756 bytes, checksum: b38e36947fda465d9d78272a81b2e947 (MD5) Previous issue date: 2015-09-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This study aimed to evaluate the quality of 'Sweet Celebration' and 'Sweet Globe' grapes, subjected to minimally processing, as well as the effectiveness of three rinsing solutions (water, sodium metabisulfite and chlorine) and pedicel cut intensity (with and without pedicel), in the conservation of these fruits during 12 days refrigerated storage to temperature at 8 ? C . The experiments were performed at Embrapa Agroind?stria de alimentos. For both experiments were applied rinse solutions, with water, sodium metabisulfite (20 mg. L-1) and chlorine (8 mg. L-1), that characterized the treatments, during the rinse step of minimally processing grapes, it being used two pedicel cut intensities:full cut and maintenance of a little fragment about 0,5 cm. Quality analyzes were performed at 0, 3, 6, 9, and 12 days of storage, and it was determined fresh weight loss cumulative , fruit firmness, color analysis, acidity, pH, total soluble solids, SST/ATT rate , sugars (sucrose , fructose and glucose) and enzymatic activity of pectinametilesterase for both cultivars. For Sweet Celebration grapes were also carried out the percentage fruit loss (9th and 12th day of storage) and anthocyanins. In 'Sweet Globe' grapes were realized total carotenoids, chlorophylls (a, b and total) and microbiological analyses. To the cultivate Sweet Celebration was possible identify berries out of commercial standard in the 9? day of storage. In the fresh weight loss cumulative, berries without pedicel had higher loss in the rinse with water from the 6th day of storage. For berries with pedicel the rinse with chlorine had the lowest fresh weight loss cumulative up 9th day of storage. For the firmness , berries without pedicel (4.44 N) had lower firmness compared to berries with pedicel (5.04 N), there wasn't significant difference between the rinsing solutions (p> 0.05). To cultivate Sweet Globe, berries with pedicel and rinsed with water had higher fresh weight loss cumulative from the 9th day of storage. In the berries without pedicel the rinse with water led to lower fresh weight loss cumulative from the 6th day of storage. There was no difference between the isolated factors rinse solutions and pedicel cut intensit to the firmness analysis. All rinse solutions were efficient in the microbiological analysis, so the minimally processed Sweet Globe grapes were in the standard established by law for human consumption / O presente trabalho objetivou avaliar a qualidade de uvas ?Sweet Celebration? e ?Sweet Globe?, submetidas ao processamento m?nimo, assim como a efetividade de tr?s solu??es enxaguantes (?gua, metabissulfito de s?dio e cloro) e da intensidade de corte do pedicelo (com e sem pedicelo), na conserva??o desses frutos, durante 12 dias de armazenamento refrigerado a 8?C. Os experimentos foram realizados na Embrapa agroind?stria de alimentos. Para ambos experimentos, foram aplicadas solu??es de enx?gue, com ?gua, metabissulfito de s?dio (20 mg. L-1) e cloro (8 mg. L-1), que caracterizaram os tratamentos, durante a etapa de enx?gue do processamento m?nimo das uvas, sendo adotadas duas intensidades de corte do pedicelo: retirada total do mesmo e manuten??o de um pequeno fragmento com aproximadamente 0,5 cm. Foram realizadas an?lises de qualidade nos 0, 3, 6, 9, e 12 dias de armazenamento, sendo determinadas a perda acumulada de massa fresca, firmeza dos frutos, an?lise de cor, acidez, pH, s?lidos sol?veis totais, rela??o SST/ATT, a??cares (sacarose, frutose e glicose) e atividade enzim?tica da pectinametilesterase para as duas cultivares. Para as uvas Sweet Celebration, foram tamb?m realizadas a porcentagem de perda de frutos (9? e 12? dia de armazenamento) e antocianinas totais. Para as uvas ?Sweet Globe?, foram realizadas as an?lises de caroten?ides totais, clorofilas (a, b e total) e an?lise microbiol?gica. Para a cultivar Sweet Celebration, foi poss?vel detectar bagas fora de padr?o comercial no 9?dia de armazenamento. Na perda acumulada de massa fresca, bagas sem pedicelo apresentaram maior perda no enx?gue com ?gua a partir do 6? dia de armazenamento. Para bagas com pedicelo o enx?gue com cloro apresentou a menor perda acumulada de massa fresca at? o 9? dia de armazenamento. Para a firmeza das bagas, bagas sem pedicelo (4,44 N) apresentaram menor firmeza, quando comparadas ?s bagas com pedicelo (5,04 N), n?o havendo diferen?a significativa entre as solu??es de enx?gue (p> 0,05). Para a cultivar Sweet Globe, bagas com pedicelo e enxaguadas com ?gua, apresentaram maior perda acumulada de massa fresca , a partir do 9? dia de armazenamento. Para bagas sem pedicelo o enx?gue com ?gua levou a menor perda acumulada de massa fresca a partir do 6? dia de armazenamento. N?o houve diferen?a entre os fatores isolados solu??es de enx?gue e intensidade de corte do pedicelo para a an?lise de firmeza. Todas as solu??es de enx?gue foram eficientes do ponto de vista microbiol?gico, estando as uvas Sweet Globe minimamente processadas dentro do padr?o estabelecido pela legisla??o para consumo humano

A dinâmica sedimentar sobre um trecho da plataforma continental leste brasileira foi analisada através da abordagem combinada entre a análise do clima de ondas (WAVEWATCH III), modelagem da propagação das ondas para águas rasas (MIKE 21 SW) e aplicação de equações que estimam o transporte de partículas em função da tensão de cisalhamento causada pelas ondas sobre o fundo marinho. Assim, baseado no clima de ondas da região, são estimados os potenciais de mobilidade sedimentar como resposta a diversas condições forçantes. O clima de ondas em frente a Ilhéus é caracterizado pela predominância de ondas do quadrante leste (60%), com 20% provenientes de sudeste e 15% de sul. Em frente a Caravelas, o espectro de ondas é caracterizado por 40% de ondas de leste, 15% do quadrante sudeste e 35% provenientes de sul. O clima de ondas é influenciado pelas descontinuidades na largura da plataforma continental nesse trecho da costa, atenuando a influência das ondas de swell provenientes do quadrante sul em frente a Ilhéus. Os resultados indicam mobilidade sedimentar até 50 m de profundidade na plataforma de Ilhéus e até 20 m na plataforma de Abrolhos ao longo do ano, no entanto, eventos esporádicos mais energéticos são capazes de mobilizar sedimentos até cerca de 200 m de profundidade na plataforma de Ilhéus e até 100 m na plataforma de Caravelas. A mobilidade de partículas de maiores dimensões, como os rodolitos da plataforma de Abrolhos, maior campo de rodolitos conhecido do mundo, foi analisada através do parâmetro de Shields, que leva em consideração tamanho e densidade das partículas. Os resultados indicam que a mobilidade dos rodolitos de Abrolhos está principalmente condicionada pela ocorrência de eventos mais energéticos, especialmente no outono. As regiões com maior mobilidade são a porção norte, próxima a quebra da plataforma e a porção centro-sul da plataforma, ambas com profundidades entre 20 e 40 m. O detalhamento do conhecimento sobre o transporte de sedimentos na plataforma continental leste brasileira contribui para o entendimento na dinâmica sedimentar na região. A morfologia complexa da plataforma, combinada com o clima de ondas incidente, são fatores fundamentais no potencial de mobilização de sedimentos. / The sedimentary dynamics on a stretch of the Brazilian continental shelf was analyzed through a combined approach between wave climate analysis (WAVEWATCH III), shallow water wave propagation modeling (MIKE 21 SW) and equations estimating the transport of particles as a function of bed shear stress by the waves. Thus, based on the wave climate of the region, sediment mobility potentials are estimated in response to several forcing conditions. Wave climate is characterized by the predominance of east waves in front of Ilhéus (60%), 20% from southeast quadrant and 15% from south. In front of Caravelas a wave spectrum is characterized by 40% from east quadrant, 15% from southeast and 35% from south. Continental shelf width discontinuity influences wave climate at Ilhéus, attenuating waves from south quadrant. The results indicate sediment mobility up to 50 m depth on the Ilhéus shelf and up to 20 m on the Abrolhos shelf along the entire year; however, more sporadic energetic events are able to mobilize sediments up to 200 m depth on the Ilhéus shelf and up to 100 m on the Caravelas shelf. Larger particle mobility, such as the rhodoliths of the Abrolhos shelf, the largest known rhodolite field in the world, was analyzed using the Shields parameter, which takes into account particle size and density. The results indicate that the mobility of Abrolhos rhodolites is mainly conditioned by the occurrence of more energetic events, especially in autumn. The regions with the greatest mobility are the northern portion, near the shelf break and the center-south portion of the shelf, both with depths between 20 and 40 m. The detailed knowledge on sediment transport in the Brazilian continental shelf contributes to the understanding of sedimentary dynamics in the region. The complex morphology of the continental shelf, combined with the incident wave climate, are fundamental factors in sediment mobilization potential.

Este trabalho foi desenvolvido dentro do contexto da hidrodinâmica da plataforma continental, com o objetivo de estudar a variabilidade subinercial das correntes no extremo norte na Plataforma Continental Sudeste brasileira. A região de estudo está localizada nas proximidades de Cabo Frio (RJ) e Arraial do Cabo (RJ) (aproximadamente 23º S / 42º W). Apesar de ser uma região bastante conhecida e estudada, em função do fenômeno da ressurgência costeira que ocorre em suas proximidades, pouco se conhece sobre a circulação subinercial nessa localidade. Devido à importância das correntes subinerciais, por serem responsáveis pelo transporte de substâncias dissolvidas ou em suspensão nas águas, tais como sedimentos, nutrientes e poluição, este trabalho tenta contribuir com um maior conhecimento desses campos de corrente, a partir da análise, no domínio do tempo e da frequência, de séries temporais de intensidade e direção das correntes e do vento local. Foram utilizados dados coletados no Projeto DEPROAS (Dinâmica do Ecossistema de Plataforma da Região Oeste do Atlântico Sul) a partir de correntógrafos e ADCP fundeados, numa radial em frente a Cabo Frio, sobre as isóbatas de 50, 100 e 200 m, bem como de uma bóia e de uma estação meteorológicas. Trabalhamos com as séries temporais do inverno de 2001 e do inverno e verão de 2003. A partir desse estudo foi possível verificar que a variabilidade subinercial domina o fluxo das correntes na região, principalmente na direção paralela à batimetria. Como no restante da PCSE, verificou-se um balanço geostrófico na direção normal à topografia, responsável por um fluxo paralelo às isóbatas, em especial na PCM e nas profundidades intermediárias afastadas das camadas limite. Foi observado que a corrente costeira, forçada por processos baroclínicos e pelo vento, típica da parte central da PCSE, atinge o extremo norte da plataforma continental, induzindo um fluxo mais frequente com sentido SE, nas camadas intermediária e de fundo, principalmente no inverno. Verificou-se também que os ventos locais têm baixa correlação com as correntes, exceto nos níveis superficiais, evidenciando a importância das forçantes remotas na dinâmica da região, somado à influência da atividade vortical ciclônica gerada na região pela Corrente do Brasil, com influência até a PCM. Evidências de eventos de ressurgência/subsidência apareceram ao longo de todos os períodos estudados, com ocorrência preferencial a W desta localidade. / This work was developed within the context of the continental shelf hydrodynamics, with the aim of studying the subtidal variability of the currents in the far north of the South Brazil Bight (SBB). The area of the study is located in the vicinity of Cabo Frio (RJ) and Arraial do Cabo (RJ) (approximately 23º S / 42º W). Despite being a well known and studied region, due to the coastal upwelling phenomenon that occurs in its vicinity, little is known about the subtidal variability in this location. Given the importance of the subtidal currents, that are responsible for the transport of substances dissolved or suspended in water, such as sediments, nutrients and pollution, this work tries to contribute to a better understanding of these current~ fields, by the analysis in the time and frequency domain, from time series of the currents and local wind intensity and direction. We used data collected in DEPROAS Project (Western South Atlantict Platform Ecosystem Dynamics) from current meters and an ADCP moored in front of Cabo Frio, on the isobaths of 50, 100 and 200 m, and from a buoy and a weather station. We worked with time series of 2001 winter and 2003 winter and summer. From this study it was found that the subtidal variability dominates the flow in the region, mainly in the parallel direction of the bathymetry. As in the rest of the SBB, there was a geostrophic balance in the normal direction of the topography, which accounts for a flow parallel to isobaths, particularly in the middle shelf and in intermediate depths of the remote boundary layer. It was observed that the coastal current forced by baroclinic processes and by the wind, typical of the central part of the SBB reaches the northern edge of the SBB, leading to a more frequent flow in the southeast direction, in the intermediate layers and the bottom, especially in the winter. It was also found that the local winds have low correlation with the currents, except on the superficial layers, indicating the importance of remote forcing in the dynamics of the region, coupled with the influence of cyclonic vortical activity in the region, generated by the Brazil Current, reaching the middle shelf. Upwelling and downwelling evidences were observed in all time series, with the preferential location occurring west of Cabo Frio.

The initial aim of this work was to evaluate processing conditions for extended shelf life (ESL) milk to have a shelf life at refrigeration temperature of 60 d. Milk was processed on a pilot-scale ultra-high-temperature processing plant and evaluated for microbial and sensory quality over 60 d at 7°C storage. Results of this study showed that lower process temperatures were preferable to minimize cooked flavors and that the minimum safe processing temperature was 134°C for 4 s as determined by the destruction of bacterial spores in the processed milk. Consumer preference panel results indicated that consumers preferred milk processed at 134°C for 4 s (those recommended in this study for ESL processing) to commercial UHT milk although there was a slight preference for pasteurized milk. The critical sensory characteristic of the processed milk was a cooked flavor, which decreased with lower processing temperature and shorter storage time; however, a significant increase in flavors that could be associated with lipolytic activity was also noted. This study highlighted deficiencies in existing methods for determining heat-stable bacterial products in thermal-processed foods. No rapid, sensitive assay for detection of heat-stable spores or lipases in milk exists. If such assays were available, it would allow processors to determine Lipase activity and bacterial spore counts before processing and direct raw milk with low spore counts and low lipolytic activity into long-shelf-life products. To this end, assays to rapidly quantitate spores and lipolytic activity in milk were developed. The lipase assay relies on the hydrolysis of p-nitrophenyl caprylate liberating a yellow color that is detected using reflectance colorimetry. The assay is sensitive to 5 mUnits/ml and is linearly correlated to spectrophotometry (r2 = 0.93) and release of titratable free fatty acids (r2 = 0.92 to 0.97). An immunocapture, enzyme-linked immunoassay coupled with a fluorescent detection system was developed for and resulted in a prototype spore assay using Bacillus stearothermophilus spores. This organism was selected because it is extremely heat resistant, is commonly found in milk, and is associated with spoilage of milk and milk products. The assay was able to quantitate spores down to 103 cfu/ml in milk and other products in about 1.5 h. Other detection limits could be set if needed.

藥品安定性試驗的目的乃在於確保該藥品具有優良藥品之特性，以保障消費者之權益。而藥品安定性試驗的品質良窳實取決於當地政府機關對其試驗之要求準則而定。近年來，我國不斷的在藥品安定性試驗方面進行研究和推擴，以提升國內市面上藥品之品質。本論文主要探討兩個主題：一是有關台灣在進行藥品安定性研究時，所規定藥品存放環境的溫度及溼度，以擬定出適合台灣環境之藥品儲存的溫溼度存放標準。二是針對藥品長期安定性試驗有效期限估算之軟體開發，使藥廠對藥品安定性試驗有效期限估算之研究分析更加便利。

Outgrowth of spoilage yeasts and moulds and post-processing acidification can limit the shelf-life of some fermented dairy products including fresh lactic curd cheeses. The possibility of using high pressure processing (HPP) for controlling these problems was investigated in a commercially manufactured fresh lactic curd cheese (pH 4.3-4.4) and fermented milk models (pH 4.3-6.5). The effects of HPP at 300 and 600 MPa on inactivation of glycolytic enzymes of lactic acid bacteria were also evaluated. Fresh cheeses made from pasteurised bovine milk using a commercial Lactococcus starter preparation were treated with high pressures ranging from 200 to 600 MPa (less than or equal to 22°C, 5 min) under vacuum packaging conditions and subsequently stored at 4°C for 8 weeks. Treatment at greater than or equal to 300 MPa substantially reduced the viable count of Lactococcus and effectively prevented the outgrowth of yeasts and moulds for 6 to 8 weeks without adversely affecting the sensory and textural attributes of the product. However, it had no significant effects (p less than 0.01) on variation of titratable acidity during storage. Fermented milk models were prepared by individually growing Lactococcus lactis subsp. lactis C10, Lactococcus lactis subsp. cremoris BK5, Streptococcus thermophilus TS1, Lactobacillus acidophilus 2400 and Lactobacillus delbrueckii subsp. bulgaricus 2517 in UHT skim milk and diluting the resulting fermented milk with UHT skim milk up to pH 6.5. Pressure treatment of the milk models at pH 5.2 resulted in substantial inhibition of post-processing acidification during storage and markedly reduced the viable count of Lactococcus at both 300 and 600 MPa and other bacteria only at 600 MPa. Treatment of the milk model at 600 MPa decreased the viable counts of Candida zeylanoides and Candida lipolytica (wildtype spoilage yeasts of lactic curd cheese, added as challenge cultures) from 105 CFU mL-1 to below the detection limit (log 0 CFU mL-1) at all pH levels tested (pH 4.3-6.5) and effectively controlled their outgrowth for 8 weeks. Treatment of milk model at 300 MPa had a similar effect only on C. zeylanoides. The viable count of C. lipolytica was reduced by 2.6, 2.4 and 2.3 logs by treatment at 300 MPa at pH levels of 4.3, 5.2 and 6.5, respectively, which subsequently recovered by 2.9, 2.8 and 3.2 logs within 3 weeks. Glycolytic enzymes of various starter bacteria showed different responses to pressure treatment. The lactate dehydrogenase in L. lactis subsp. lactis and Lb. acidophilus was quite resistant to pressures up to 600 MPa, but it was almost completely inactivated in S. thermophilus at pressure levels as low as 300 MPa. The â-galactosidase in Lb. acidophilus was more pressure stable than â-galactosidase in S. thermophilus and Phospho-â-galactosidase in L. lactis subsp. lactis. The findings of this study suggests HPP at 300-600 MPa as an effective method for controlling the outgrowth of some spoilage yeasts and moulds in fresh lactic curd cheeses. The results obtained with selected lactic acid bacteria in fermented milk models can be used to assist in establishing HPP operating parameters for development of new generation cultured dairy products, of reduced acidity and extended shelf-life.

<p>Detta examensarbete beskriver problem och hinder som uppstår vid nyföretagande och entreprenörskap. Arbetet initierades i en period som karaktäriserades av stora nedskärningar inom den svenska högteknologi industrin och väldigt få examensarbeten var tillgängliga.</p><p>Den entreprenöriella processen och tankesättet har varit väldigt tongivande för det svenska samhället. Denna anda har producerat flera stora internationella storföretag. Även om inte alla entreprenörer når de internationella företag i storlek så har småföretagen en minst lika viktig roll för utvecklingen av samhället med det kreativa tankesättet och synen på affärsmöjligheter.</p><p>Rapporten består av litteraturstudie och en fallstudie Hermes Technologies. Med hjälp av modeller kring den entreprenöriella processen, teorier om de psykografiska förhållandena på högteknologiska marknader samt teorier kring skapandet av en markand och prissättning samt analysverktygen Affärsplattformen har en teoretisk bas byggts upp för att sedan appliceras på en fallstudie.</p><p>Teorin framhäver hur viktigt upptäckten av affärsmöjligheten är för framgång tillsammans med ett kreativt hanterande av resurserna.</p><p>Fallstudien består av uppstarten till grundandet av ett högteknologiskt utvecklingsföretag inom marknaden för Electronic Shelf Labeling system, ESL-system. Casestudien genomfördes vid ProNova Science Park i Norrköping som en del i pilotprojektet ”FörInkubatorn” vilket senare utvecklades till Drivbänken. Genom att tillämpa teorin på fallstudien framkom det att flera faktorer för att med framgång genomföra grundandet av Hermes Technologies saknades eller var bristfälliga.</p><p>Här framgår även hur grundandet av Hermes Technologies har genomförts. Vidare framförs hur detaljhandeln ställer sig inför införandet av ESL-system istället för dagens pappersmarkeringar.</p><p>Analysen klargjorde vilka faktorer som låg till grund för ett misslyckade samt vilka åtgärder som krävdes för att uppnå en mer tillfredsställande nivå. Erfarenhet var den ensamt största bristfälliga faktorn som resulterade i att projektet inte lyckades. För att kunna påverka denna faktor krävs det ofta flertaliga försök inom nyföretagande.</p><p>Resultatet påvisar att de teorier, som används i litteraturstudien, bör användas tillsammans med varandra för att uppnå bästa resultat vid nystartandet av ett företag. Detta kommer av att man på detta vis belyser problemet från flera infallsvinklar vilket ytterligare klargör de hinder som kan uppkomma eller har uppstått.</p>

<p>Systems development projects and their documents are more or less standardized and can mainly be applied on systems that are supposed to be built from scratch, or updated. In pace with the number of IT-systems are increasing worldwide there is no need for every organization to build their own IT-system. Nowadays it is also possible to purchase licenses which allow the purchaser to modify or add functions to the system. Along with those changes, there have been an increased amount of “rapid development methods” such as Agile and “Quick and Dirty” solutions, but these methods and perspectives are mainly focusing on entire systems development processes, as the old ones, but quicker.</p><p>If a company purchases an off-the-shelf system with source code available, there is no real need to go through a proper systems development process. During interviews with a small company that has acquired a system as mentioned above, the researcher realized that only one single document is needed, the requirements specification. Today’s requirements specifications can be either well detailed or less, but a project still needs the details specified. Combining a known agile development process with IEEE’s standardized requirements specification, a new way to proceed with projects based on one single document (the requirements specification) has been made. This document also has a focus on simplicity for the inexperienced readers, but with the depth that every developer has got a use for.</p>

Detta examensarbete beskriver problem och hinder som uppstår vid nyföretagande och entreprenörskap. Arbetet initierades i en period som karaktäriserades av stora nedskärningar inom den svenska högteknologi industrin och väldigt få examensarbeten var tillgängliga. Den entreprenöriella processen och tankesättet har varit väldigt tongivande för det svenska samhället. Denna anda har producerat flera stora internationella storföretag. Även om inte alla entreprenörer når de internationella företag i storlek så har småföretagen en minst lika viktig roll för utvecklingen av samhället med det kreativa tankesättet och synen på affärsmöjligheter. Rapporten består av litteraturstudie och en fallstudie Hermes Technologies. Med hjälp av modeller kring den entreprenöriella processen, teorier om de psykografiska förhållandena på högteknologiska marknader samt teorier kring skapandet av en markand och prissättning samt analysverktygen Affärsplattformen har en teoretisk bas byggts upp för att sedan appliceras på en fallstudie. Teorin framhäver hur viktigt upptäckten av affärsmöjligheten är för framgång tillsammans med ett kreativt hanterande av resurserna. Fallstudien består av uppstarten till grundandet av ett högteknologiskt utvecklingsföretag inom marknaden för Electronic Shelf Labeling system, ESL-system. Casestudien genomfördes vid ProNova Science Park i Norrköping som en del i pilotprojektet ”FörInkubatorn” vilket senare utvecklades till Drivbänken. Genom att tillämpa teorin på fallstudien framkom det att flera faktorer för att med framgång genomföra grundandet av Hermes Technologies saknades eller var bristfälliga. Här framgår även hur grundandet av Hermes Technologies har genomförts. Vidare framförs hur detaljhandeln ställer sig inför införandet av ESL-system istället för dagens pappersmarkeringar. Analysen klargjorde vilka faktorer som låg till grund för ett misslyckade samt vilka åtgärder som krävdes för att uppnå en mer tillfredsställande nivå. Erfarenhet var den ensamt största bristfälliga faktorn som resulterade i att projektet inte lyckades. För att kunna påverka denna faktor krävs det ofta flertaliga försök inom nyföretagande. Resultatet påvisar att de teorier, som används i litteraturstudien, bör användas tillsammans med varandra för att uppnå bästa resultat vid nystartandet av ett företag. Detta kommer av att man på detta vis belyser problemet från flera infallsvinklar vilket ytterligare klargör de hinder som kan uppkomma eller har uppstått.

Early 1990s to late 2000s freshening (ΔS ≈ -0.001–0.002) and warming (Δθ ≈ 0.02°C–0.035°C) of bottom waters was detected in the southern Pacific Ocean, and Ross Sea source waters progressively freshened during the past four decades. This study investigates potential freshwater anomaly sources and quantifies their effect. Glacial melt water inputs to the GCT increased by 1.3 km^3 per decade (1976– 2007), more rapidly so after 2000 (6.8 km^3 per decade), freshening local Shelf Water by 0.0004 per decade. Lighter basal melt inputs to the LAT started in 1994 and also picked up after 2000 to 14.9 km^3 per decade, lowering the local Antarctic Surface Water salinity by -0.017 per decade. Upstream in the Amundsen Sea surface water freshened by -0.03 per decade (1994–2007) mostly (50%) from larger melt water inputs from the Pine Island (17.7 km^3 per decade) and Dotson (14.8 km^3 per decade) glaciers. Two decades of steady (1978-2000) strengthening of sea ice productivity (200 km^3 per decade) within the Ross Sea Polynya suddenly reversed to weakening (-98.6 km^3 per decade) and resulted in Shelf Water freshening (-0.02 per decade) thereafter. To fully account for the observed variability in Ross Sea waters, the progressive (1992- 2011) adjustment of the density field and induced advective contributions are estimated based on a simplified three-layer stratification. Eastern (western) inflow (outflow) of light surface (dense shelf) water increased by 28% (15%) to 1.11 Sv (1.01 Sv) by 2011; whereas a sluggish intermediate inflow (0.02 Sv) of Modified Circumpolar Deep Water turned into outflow after 2007, thus contributing 0.09 Sv by 2011 to the ventilation of deep waters farther offshore. The estimated evolution of overturning and advective salt fluxes in the Ross Sea yield overall freshening of water masses similar to those derived from observations. Volumetric mean salinities declined at -0.07 per decade for Antarctic Surface Water, -0.05 per decade for Modified Circumpolar Water, and -0.03 per decade for Shelf Water. Outflow intensification of Shelf Water mixtures is also consistent with bottom water property changes (freshening and warming) measured farther downstream in the southern Pacific Ocean.