High nitrogen discharge from the municipal wastewater is a major concern for the South Australian Government, primarily due to negative impacts on the marine environment. Therefore, under the South Australian Environmental Improvement Program, (SA EIP), all metropolitan wastewater treatment plants have been reconfigured to achieve enhanced nitrogen removal. Secondary treatment (denitrification process) at the metropolitan wastewater treatment plants must be optimised to meet the discharge guideline of 10 mg/L total nitrogen. However, secondary treatment at some plants is carbon limited (low C/N ratio), and external carbon supplementation is required to meet this discharge guideline. Molasses provides the current external carbon source at two plants. It is relatively inexpensive, but other carbon sources, particularly industrial waste streams, may be more attractive, due to the potentially lower material cost, as it is practically free, and environmentally friendly. Potato starch and sonicated return activated sludge (RAS) were considered. In this study, the bioavailability of the soluble carbon in potato starch and ultrasound treated RAS were assessed. The associated objective was to investigate the potential of both carbon sources as an external carbon donor for the denitrification zone of wastewater treatment plants to economically improve biological nitrogen removal. The economic analysis was performed using mainly United States dollars and the fixed capital investments and total capital costs were converted to Australian dollars. This was due to the United States dollars currency quotes obtained for the materials and unit operations required. SCOD from the three sources was quantified and preliminary results were presented. Molasses provided the highest SCOD release of 1.1285 x 10⁶ mg-SCOD/L, sonicated RAS produced 5.6 to 68.4 times the SCOD release of the untreated RAS (35.6 mg-SCOD/L) depending on the ultrasound intensity and treatment time, while the highest soluble carbon release obtained using potato starch was 809 mg-SCOD/L (using 20.9 g/100 mL potato starch concentration). Based on the experimental SCOD results, batch denitrification tests using the proposed carbon sources were carried out. The nitrogen removal efficiency at low dose (12.48 mg-SCOD/L) using molasses, potato starch and sonicated RAS were 77.54%, 57.24%, and 72.76% respectively, whilst at high dose (124.80 mg-SCOD/L) were 94.04%, 66.32%, and 92.10% correspondingly. In similar order of the proposed carbon sources, the nitrate removal rates for the first phase denitrification with low dose were 1.44, 1.16, and 1.18 mg-NO₃ − /h respectively, whilst the nitrate removal rate of the first phase denitrification with high dose improved to 2.01, 1.26, and 1.96 mg-NO₃ −/h correspondingly. From the denitrification test results, molasses proved to be the optimal carbon source in terms of nitrate removal. However sonicated RAS possesses similar denitrification performance and may be a suitable alternative. An economic analysis for sonicated RAS Option 2 confirmed it as the most viable substitute. The time to recover the initial investment (payback period) is approximately 6.5 years and the breakeven point is approximately 8 years. Both denitrification tests and economic analyses demonstrate that sonicated RAS may be a viable and attractive substitute for the molasses. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1337059 / Thesis (M.Eng.Sc.) - University of Adelaide, School of Chemical Engineering, 2008

High nitrogen discharge from the municipal wastewater is a major concern for the South Australian Government, primarily due to negative impacts on the marine environment. Therefore, under the South Australian Environmental Improvement Program, (SA EIP), all metropolitan wastewater treatment plants have been reconfigured to achieve enhanced nitrogen removal. Secondary treatment (denitrification process) at the metropolitan wastewater treatment plants must be optimised to meet the discharge guideline of 10 mg/L total nitrogen. However, secondary treatment at some plants is carbon limited (low C/N ratio), and external carbon supplementation is required to meet this discharge guideline. Molasses provides the current external carbon source at two plants. It is relatively inexpensive, but other carbon sources, particularly industrial waste streams, may be more attractive, due to the potentially lower material cost, as it is practically free, and environmentally friendly. Potato starch and sonicated return activated sludge (RAS) were considered. In this study, the bioavailability of the soluble carbon in potato starch and ultrasound treated RAS were assessed. The associated objective was to investigate the potential of both carbon sources as an external carbon donor for the denitrification zone of wastewater treatment plants to economically improve biological nitrogen removal. The economic analysis was performed using mainly United States dollars and the fixed capital investments and total capital costs were converted to Australian dollars. This was due to the United States dollars currency quotes obtained for the materials and unit operations required. SCOD from the three sources was quantified and preliminary results were presented. Molasses provided the highest SCOD release of 1.1285 x 10⁶ mg-SCOD/L, sonicated RAS produced 5.6 to 68.4 times the SCOD release of the untreated RAS (35.6 mg-SCOD/L) depending on the ultrasound intensity and treatment time, while the highest soluble carbon release obtained using potato starch was 809 mg-SCOD/L (using 20.9 g/100 mL potato starch concentration). Based on the experimental SCOD results, batch denitrification tests using the proposed carbon sources were carried out. The nitrogen removal efficiency at low dose (12.48 mg-SCOD/L) using molasses, potato starch and sonicated RAS were 77.54%, 57.24%, and 72.76% respectively, whilst at high dose (124.80 mg-SCOD/L) were 94.04%, 66.32%, and 92.10% correspondingly. In similar order of the proposed carbon sources, the nitrate removal rates for the first phase denitrification with low dose were 1.44, 1.16, and 1.18 mg-NO₃ − /h respectively, whilst the nitrate removal rate of the first phase denitrification with high dose improved to 2.01, 1.26, and 1.96 mg-NO₃ −/h correspondingly. From the denitrification test results, molasses proved to be the optimal carbon source in terms of nitrate removal. However sonicated RAS possesses similar denitrification performance and may be a suitable alternative. An economic analysis for sonicated RAS Option 2 confirmed it as the most viable substitute. The time to recover the initial investment (payback period) is approximately 6.5 years and the breakeven point is approximately 8 years. Both denitrification tests and economic analyses demonstrate that sonicated RAS may be a viable and attractive substitute for the molasses. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1337059 / Thesis (M.Eng.Sc.) - University of Adelaide, School of Chemical Engineering, 2008

The inflammatory arthritis Ankylosing Spondylitis (AS) is linked to the human leucocyte antigen HLA-B27. HLA-B27 is thought to drive AS because it misfolds during assembly in the endoplasmic reticulum (ER), inducing ER cell stress. Modulating HLA-B27 folding in the ER is therefore a therapeutic target pathway. The recent discovery of polymorphisms in the ER-resident peptidase ERAP1 that can impact on HLA-B27 and AS, makes ERAP1 one such target. Exosomes are small, typically 50-200 nm sized particles, formed in the endosomal recycling pathway, which can be released into the extracellular environment. Exosomes have a wide range of biological activities depending on the cell type of origin, and on the delivered cargo, which can include bio-active proteins, lipids, mRNA and miRNA. There is interest in the use of exosomes as drug delivery agents. Here, exosomes were studied as a delivery agent to modulate ERAP1, as a potential therapeutic tool for the treatment of AS. Exosomes, isolated from cell lines including CEM and Jurkat (T cell lineage), Jesthom (B cell lineage), U937 (monocyte lineage) and the epithelial HeLa cell line, were characterized by nanoparticle tracking analysis, flow cytometry and immunoblotting using markers including CD9, CD63, CD81 and TSG101. Differential expression of these markers in the immune cell lines indicated the complexity of defining exosomes. EVs were then tested using cell penetrating peptides, electroporation, lipid transfection and sonication for their ability to load FITC-siRNA or FITC-antibody as cargo. Significantly, post-loading RNase A or trypsin incubation demonstrated that many techniques do not lead to efficient cargo loading of exosomes. Sonication proved the most effective technique, with up to 30% efficiency. Loading of exosomes with ERAP1-targetted siRNA did not however lead to notable ERAP1 inhibition. The data indicates that external loading of exosomes with cargo remains a significant challenge in developing exosomes as therapeutic tools.

SANTOS, Jessika Gonçalves dos. Aplicação de ultrassom em mangas ‘Tommy Atkins’ minimamente processadas: aspectos fisiológicos e de qualidade. 2013. 80 f. Dissertação (Mestrado) - Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Bioquímica. Fortaleza-CE, 2013. / Submitted by Eric Santiago (erichhcl@gmail.com) on 2016-05-25T13:54:48Z No. of bitstreams: 1 2013_dis_jgsantos.pdf: 1622822 bytes, checksum: 979c22c014ca659c040db7ab775062a8 (MD5) / Approved for entry into archive by José Jairo Viana de Sousa (jairo@ufc.br) on 2016-05-25T22:47:10Z (GMT) No. of bitstreams: 1 2013_dis_jgsantos.pdf: 1622822 bytes, checksum: 979c22c014ca659c040db7ab775062a8 (MD5) / Made available in DSpace on 2016-05-25T22:47:10Z (GMT). No. of bitstreams: 1 2013_dis_jgsantos.pdf: 1622822 bytes, checksum: 979c22c014ca659c040db7ab775062a8 (MD5) Previous issue date: 2013 / Currently is growing to search for healthier eating with that comes the possibility for inclusion of fruits and vegetables in the diet. Mango fruit is widely consumed and produced in Brazil and fresh – cut (FC) represents convenience because them are products that comes ready for consumption without loss of time with operations peeling, cutting, etc., and these steps there is a stress tissue leading to generation of free radicals that can lead to a degradation fruit, so it is necessary a type of processing that minimize the loss of quality and yet ensures the safety of the final product. In this context arises the ultrasound (US) that is an emerging technology that has potential for exploitation and utilization. The ultrasonic waves cause primarily tissue compression and expansion, whose the results will depend on the food matrix. This study aimed to evaluate the effect of US in quality of FC 'Tommy Atkins' mangoes, analyzing the antioxidant defense system, metabolism oxidative compounds and the activity of cell wall hydrolytic enzymes. The samples were exposed to 25 kHz frequency sonication for 30 minutes in US bath. It was observed tha t the treatment caused a reduction in titratable acidity (TA) and soluble solids (SS), but after seven days of storage at 4 o C, maintaining the high SS/TA ratio (46.54). The sugars was also reduced (15%) and water incorporated (4.6%), which indicates a change in cell structure, that can be evidenced by decreased in firmness after seven days (from 241.73 to 139.38 N) and increased activity of pectinmethylesterase. The color wa s degraded after seven days (ΔL = 3.24 and ΔC = 4.42), resulting in darkening, but the control samples also darkened. In relation to the antioxidant system, there were no significant changes in order to be a beneficial factor compared to other preservation methods, particularly only to the loss of vitamin C (328.10 to 190.18 mg ascorbic acid/kg weight fresh (WF), still there was a reduction in lipid peroxidation of membrane (from 23.58 to 18 η mol MDA/g WF). It concluded to base on this study, FC 'Tommy Atkins' mango has its drawbacks on firmness and color, however, did not affect severely the enzymatic and non – enzymatic antioxidant x systems, and lead to a reduction in sugar content. However, it is clear the necessitate to explore the application of this technology in post harvest fruits physiology as well as to optimize and to combine the technique with other processes that reduce their negative effects and promote the positives. / Atualmente é crescente a busca por uma alimentação mais saudável e com isso surge a possibilidade da inclusão de frutos e hortaliças nos hábitos alimentares. A manga é um fruto bastante consumido e produzido no Brasil e os minimamente processados (MP) representam praticidade porque são produtos que já vem prontos para consumo, sem perda de tempo com operações de descasque, corte, etc. Durante as etapas de processamento há um estresse dos tecidos, que leva à geração de radicais livres que podem causar a degradação do fruto, por isso, se faz necessário a utilização de tratamentos capazes de minimizar as perdas de qualidade. Nesse contexto surge o ultrassom (US) que é uma tecnologia emergente com potencial de exploração e utilização. As ondas ultrassônicas provocam principalmente a compressão e expansão dos tecidos, cujos resultados vão depender da matriz alimentar. Este trabalho teve como objetivo avaliar o efeito do US na qualidade de mangas ‘Tommy Atkins’ MP, analisando o sistema de defesa antioxidante, componentes do metabolismo oxidativo e a atividade de enzimas hidrolíticas da parede celular. Para tanto, as amostras foram expostas à sonicação na frequência de 25 kHz por 30 minutos em banho de US. Observou–se que o tratamento causou uma redução na acidez titulável (AT) e nos sólidos solúveis (SS), porém após sete dias de armazenamento a 4 oC, manteve elevada a relação SS/AT (46,54). Os teores de açúcares também foram reduzidos (15%), bem como água foi incorporada (4,6%), o que mostra uma modificação na estrutura celular, que pode ser comprovada pelo decréscimo na firmeza após sete dias (de 241,73 para 139,38 N) e aumento na atividade da pectinametilesterase. A cor foi degradada após sete dias (∆L = 3,24 e ∆C = 4,42), resultando em escurecimento, contudo as amostras controle também escureceram. Em relação ao sistema antioxidante, não houve grandes modificações, tendo em vista ser um fator benéfico se comparado a outros métodos de conservação, com destaque apenas para a perda de vitamina C (de 328,10 para 190,18 mg ácido ascórbico/kg matéria fresca (MF)). Houve ainda redução na peroxidação de lipídios de membrana com o tratamento (de 23,58 para 18 ηmol MDA/g MF). Conclui–se com base neste estudo, que a aplicação de US em mangas ‘Tommy Atkins’ MP tem seus pontos negativos na firmeza e na cor, contudo, não afetou drasticamente os sistemas antioxidante enzimático e não enzimático, além de levar a uma redução no teor de açúcar. Todavia, fica claro a necessidade de explorar esta tecnologia na fisiologia pós–colheita de frutos, assim como, otimizar e combinar a técnica com outros processos que diminuam seus efeitos negativos e favoreçam os positivos.

This thesis presents the use of ultrasonic radiation for the digestion of biodiesel samples (B100) for the determination of metal ions by electrochemical stripping techniques (potentiometric and voltammetric) using a glassy-carbon electrode modified with a mercury film. Biodiesel samples (50 mg) were subjected to digestion in glass tubes with a mixture of 3.0 mL of concentrated HCl and 4.4 ml H2O2 (30%), placed in an ultrasonic bath (40 kHz and 120 W) exactly on the higher intensity region (piezoelectric) of the ultrasound and sonicated for 40 min at room temperature (~ 20°C). These parameters were optimized using the chemometric technique of factorial design for the analysis of biodiesel samples spiked with ethanolic standard solutions of zinc, cadmium, lead and copper. Recovery values between 97 and 107% were observed in the optimal conditions found to zinc, cadmium, lead and copper. Residual carbon content (RCC) values after digestions were in the range of 3.05 to 3.75%. The simultaneous determination of zinc, cadmium, lead and copper using anodic stripping voltammetry revealed limit of detection (LD) values of 44 ng kg -1; 30 ng kg -1; 145 ng kg -1; 96 ng kg -1, respectively. Additionally, biodiesel samples (100 mg) were also treated in glass tubes with a mixture of 1.59 mL concentrated HCl and 3.0 ml H2O2 (30%) under sonication of an ultrasonic probe (sonication time 12.5 min, 25% amplitude, pulse of 5.0 s and time interval of 2.0 s). The parameters were also optimized employing the chemometric technique of factorial design for the analysis of spiked samples. Recovery values between 95 and 104% were observed in the satisfactory condition for zinc, cadmium, lead and copper. RCC values after digestions comprised values between 0.43 to 0.54%. For the simultaneous determination of zinc, cadmium, lead and copper using anodic stripping voltammetry obtained in the digestates employing the ultrasonic probe, the LD values found respectively for zinc, cadmium and copper were 25 ng kg -1 and for lead 56 ng kg -1. All results obtained using assisted digestion by ultrasonic bath and / or ultrasonic probe indicate that the proposed methods for the biodiesel sample preparation presents a comparable performance to traditional methods of digestion using acid blends, or yet digestions assisted by microwave radiation. / Esta tese apresenta o emprego da radiação ultrassônica para a digestão de amostras de biodiesel (B100) para a determinação dos íons metálicos por técnicas eletroquímicas de redissolução (potenciomérica e voltamétrica) empregando eletrodo de carbono vítreo modificado com filme de mercúrio. Amostras de biodiesel (50 mg) foram submetidas a digestão em tubos de vidro, com uma mistura de 3,0 mL de HCl concentrado e 4,4 ml de H2O2 (30%), colocados em um banho ultrassônico (40 kHz e 120 W) exatamente sobre a região de intensidade mais elevada (piezoelétricos) do ultrassom e sonicada durante 40 min a temperatura ambiente (~ 20 ºC). Estes parâmetros foram otimizados empregando-se a técnica quimiométrica de planejamento fatorial para a análise de amostras de biodiesel enriquecidas com soluções padrão etanólicas de zinco, cádmio, chumbo e cobre. Valores de recuperação entre 97 e 107 % foram verificados na condição de ótimo encontrada para zinco, cádmio, chumbo e cobre. Valores de RCC (teor de carbono residual) nos digeridos encontraram-se na faixa de 3,05-3,75%. A determinação simultânea de zinco, cádmio, chumbo e cobre empregando voltametria de redissolução anódica revelou valores de limite de detecção (LD) de 44 ng Kg-1; 30 ng Kg-1; 145 ng Kg-1 e 96 ng Kg-1, respectivamente. Adicionalmente, amostras de biodiesel (100 mg) também foram tratadas em tubos de vidro com uma mistura de 1,59 mL de HCl concentrado e 3,0 ml de H2O2 (30%), sob sonicação de uma sonda ultrassônica (tempo de sonicação de 12,5 minutos, amplitude de 25 %, tempo de pulso de 5,0 s em intervalo 2,0 s). Os parâmetros também foram otimizados empregando-se técnica quimiométrica de planejamento fatorial para a análise de amostras enriquecidas. Valores de recuperação entre 95 e 104% foram verificados nas condições satisfatórias para os íons metálicos zinco, cádmio, chumbo e cobre. Valores de RCC após as digestões compreendem valores entre 0,43-0,54 %. Na determinação simultânea de zinco, cádmio, chumbo e cobre empregando voltametria de redissolução anódica nos digeridos obtidos após tratamento com a sonda ultrassônica, os valores de LD encontrados respectivamente para os íons metálicos zinco, cádmio e cobre foram de 25 ng Kg-1 e para íon chumbo de 56 ng Kg-1. Todos os resultados obtidos empregando digestão assistida por banho de ultrassom e /ou sonda de ultrasssom indicam que os métodos propostos para o preparo de amostra de biodiesel apresentam um desempenho comparável aos metodos tradicionais de digestão que utilizam misturas ácidas, ou ainda as digestões assistidas por radiações de microondas. / Doutor em Química

This Master’s thesis deals with isolating carbon nanoparticles from South Moravian lignite using "green" – environmentally friendly methods. This method consists of mechanical-chemical-thermal stressing of lignite using ultrasound, hydrogen peroxide and high temperatures. This work aims to develop recommendations on coal as a source of carbon nanoparticles and based on these recommendations, to design procedures for isolating nanoparticles from South Moravian lignite using environmentally friendly methods. The identified procedures were designed to meet carbon-based particles with an oxidized surface. From the results we can deduct that it is possible to use South Moravian lignite for the preparation of carbon nanoparticles. However, it will be necessary to modify the process further and also to optimize the purification of the obtained particles.

La méthanisation est un processus biologique de transformation des matières organiques libérant principalement du méthane et du dioxyde de carbone. Cette technologie connaît un essor important pour la production de biométhane, source d’énergie renouvelable. Elle présente cependant des rendements de dégradation faibles lorsque de la biomasse lignocellulosique est utilisée comme matière première. Pour optimiser son rendement, la paille de blé a été sélectionnée comme biomasse représentative et l’impact sur la méthanisation de chacune des fractions (extractibles, hémicelluloses, cellulose et lignine) a été évalué. Une biomasse de synthèse a été construiteà partir des constituants pures de la paille de blé afin d’évaluer l’impact des interactions lignine-holocellulose. Le potentiel de méthanisation de différentes molécules phénoliques,provenant de la dégradation de la lignine, a été déterminé. Elles inhibent la méthanisation à l’exception de trois d’entres elles qui présentent un rendement de méthanisation élevé : les acides vanillique, l’acide férulique et le syringaldéhyde. Différents prétraitements physique (le chauffage par échangeur thermique ou par irradiation aux micro-ondes ainsi que la sonication et le raffinage papetier) et chimique (la soude, l’ammoniaque et l’ozone) ont ensuite été sélectionnés, et leurs impacts sur lacomposition de la paille et sa méthanisation ont été mesurés. Les meilleurs rendements de méthanisation ont été obtenus suite à l’exposition aux micro-ondes en présence de soude. / Methanization or anaerobic digestion is a biological process to transform organicmatter into a gas mixture composed by a majority of methane and carbon dioxide. Thistechnology is developing rapidly for the production of biomethane as renewable energysource. However this biotechnological route has low performances when lignocellulosicbiomass is used as raw material.Wheat straw has been chosen as typical biomass and the role of each lignocellulosicfraction (extractives, cellulose, hemicelluloses and lignin) has been determined on theperformance of anaerobic digestion. A synthetic biomass has been built with different pureconstituents of the wheat straw to assess the impact of holocellulose-lignin interactions onmethanization. Then methane potential of various lignin degradation products (phenolicmolecules) has been studied. Majority of them have been shown an inhibitory effect butthree of them have been converted to methane: ferulic and vanillic acids andsyringaldehyde.Various physical pretreatments (heating, microwave irradiation, sonication andrefining) and chemical pretreatments (sodium hydroxide, ammonia and ozone) have beenselected to prepare the biomass to anaerobic digestion and their impacts on wheat strawcomposition have been evaluated. The best methanization yield has been obtained afterpretreatments by sodium hydroxide heating by microwave irradiation.

In this study, the effect of ultrasonic treatment (UST) parameters such as amplitude, sonication time, and melt temperature on microstructure and microhardness of Al 6061 alloy is evaluated. The effect of UST on the dispersion of tungsten disulfide (WS2) and carbon nanotubes (CNT) as reinforcement particles in Al 6061 during casting is also studied. The cast Al 6061 with UST demonstrated 32% grain size reduction and 8% increase in the microhardness for optimum processing conditions. The cavitation process induced by UST is responsible for the refinement in microstructure and increase of hardness by enhancing the degassing and nucleation process. UST treated 6061 Al alloy demonstrated Hall-Petch relationship for all processing conditions. The UST process also aids in excellent dispersion of WS2 and CNT as reinforcement particles. UST treated WS2 and CNT reinforced Al 6061 composites displayed improved wear resistance as compared to samples without cavitation.

No description available.

The aim of this study was to evaluate the effect of ultrasound application (US, bath 25 kHz, 500 W) on physical, chemical and microbiological properties of Italian-type fermented sausage during processing and storage. The US was used after mass stuffing in synthetic packaging during 0 min, 3 min, 6 min and 9 min of application. Completely randomized design was adopted (DIC) and the data submitted to analysis of variance (ANOVA) to test the significance of the variables. Further, a Pearson´s correlation analysis was performed and the differences among the averages were calculated by using the Tukey s test (p≤0.05). Some data were subjected to multivariate analysis of variance (MANOVA), complemented by the technique of principal components analysis. The parameters of fermented sausage evaluated included counts of lactic acid bacteria (Pediococcus pentosaceus and Pediococcus acidilactici) and Microccocaceae (Staphylococcus xylosus and Staphylococcus carnosus), aw, pH, color (CIE L * a * b *), lipid oxidation (peroxide value and TBARS ), protein oxidation (thiol), total heme pigments, non-heme iron and metmyoglobin, during processing (days 0, 2, 15 and 28) and along the shelf-life (after 1, 30 and 120 days). Texture parameters (cohesiveness, flexibility, chewiness and hardness) were evaluated in fermented sausages after 90 days of storage and the formation of volatile compounds at the beginning (day 1) and the end of storage (after 120 days). US had a positive effect on the growth of bacteria in some processing steps and storage conditions (p<0.05), especially on Micrococcaceae (Staphylococcus xylosus and Staphylococcus carnosus) with 9 min of US exposure. The pH, aw and color parameters were not significantly affected by the US (p>0.05). The oxidation of lipids and proteins was accelerated with US application, but maintaining acceptable values in the final product. The total heme pigments, non-heme iron and metmyoglobin were affected by sonication (p<0.05) and influenced the oxidative reactions and vice versa. The canonical discriminant analysis separated the samples into groups according to the day of evaluation and not according to the treatments. Therefore, due to the predominant effect of processing time / storage in fermented sausage characteristics a lack of significant effect of sonication time was observed. The US did not significantly affect texture profile parameters (p>0.05), but hardness and chewiness tended to reduce with sonication. Regarding the volatiles were no statistical differences between treatments (p>0.05), except for ethanol and hexanol (p<0.05) at the beginning of storage. The US has affected the growth curve of bacteria, accelerating or inhibiting their growth that some points, and has the potential to be explored in improving the texture and red color formation of fermented sausages. The lack of pronounced effect on US some characteristics was primarily attributable to acoustic parameters adopted, low exposure time to the US, fermented sausage consistency, mass heterogeneity and quantity / fat composition, among other factors. Thus, it is suggested to further studies to assess the potential of using the US as emerging technology in the fermented sausage manufacturing. / O objetivo deste trabalho foi avaliar o efeito do uso de banho de ultrassom (US, 25 kHz, 500 W) sobre características físicas, químicas e microbiológicas de salame tipo Italiano durante seu processamento e no armazenamento. O US foi usado logo após embutimento da massa na tripa durante diferentes tempos de exposição (0 min controle não sonicado, 3 min, 6 min e 9 min). Foi adotado delineamento inteiramente casualizado (DIC) e os dados submetidos à análise de variância univariada (ANOVA) para testar a significância das variáveis. Posteriormente foi feita análise de correlação de Pearson e as diferenças entre as médias calculadas pelo teste de Tukey (p≤0,05). Alguns dados foram sujeitos à análise de variância multivariada (MANOVA), complementada pela técnica de componentes principais. As avaliações realizadas incluíram contagens das bactérias lácticas (Pediococcus pentosaceus e Pediococcus acidilactici) e Microccocaceae (Staphylococcus xylosus e Staphylococcus carnosus), aw, pH, cor (CIE L*a*b*), oxidação lipídica (índice de peróxidos e TBARS), oxidação proteica (tiol), pigmentos heme totais, ferro não heme e metamioglobina durante o processamento (dias 0, 2, 15 e 28) e ao longo da vida de prateleira (após 1, 30 e 120 dias). Parâmetros de textura (coesividade, flexibilidade, mastigabilidade e dureza) foram avaliados nos salames após 90 dias de armazenamento e a formação de compostos voláteis no início (1º dia) e fim do armazenamento (120º dia). O US exerceu efeito positivo no crescimento das bactérias em algumas etapas do processamento e do armazenamento (p<0,05), principalmente sobre Micrococcaceae (Staphylococcus xylosus e Staphylococcus carnosus) e com 9 min de exposição. O pH, aw e parâmetros de cor não foram afetados de maneira significativa pelo US (p>0,05). A oxidação de lipídios e de proteínas foi acelerada com o US, contudo mantendo valores aceitáveis. Os pigmentos heme totais, ferro não heme e metamioglobina foram afetados pela sonicação (p<0,05) e influenciaram nas reações oxidativas. A análise discriminatória canônica no processamento e no armazenamento separou as amostras em grupos de acordo com o dia de análise e não de acordo com os tratamentos, tanto em função do efeito preponderante do tempo de processamento/armazenamento nas características do salame quanto por falta de efeito expressivo do tempo de sonicação. O US não afetou significativamente parâmetros do perfil de textura (p>0,05), contudo dureza e mastigabilidade mostraram tendência a reduzirem com a sonicação. Com relação aos compostos voláteis, não foram encontradas diferenças estatísticas entre os tratamentos (p>0,05), com exceção de etanol e hexanol (p<0,05) no início do armazenamento. O US afetou a curva de crescimento das bactérias, acelerando ou inibindo seu crescimento em alguns pontos, e apresenta potencial a ser explorado na melhora da textura e na formação de cor vermelha do salame. A falta de efeito pronunciado do US sobre algumas características foi atribuída principalmente aos parâmetros acústicos adotados, baixo tempo de exposição ao US, consistência do salame, heterogeneidade da massa e quantidade/composição da gordura, entre outros fatores. Com isso, sugere-se a realização de mais estudos que avaliem as potencialidades do uso do US como tecnologia emergente na fabricação de salame.