An inverted base pavement is a new pavement structure that consists of an unbound aggregate base between a stiff cement-treated foundation layer and a thin asphalt cover. Unlike conventional pavements which rely on upper stiff layers to bear and spread traffic loads, the unbound aggregate inter-layer in an inverted base pavement plays a major role in the mechanical response of the pavement structure. Traditional empirical pavement design methods rely on rules developed through long-term experience with conventional flexible or rigid pavement structures. The boundaries imposed on the unbound aggregate base in an inverted pavement structure change radically from those in conventional pavements. Therefore, current empirically derived design methods are unsuitable for the analysis of inverted base pavements. The present work documents a comprehensive experimental study on a full-scale inverted pavement test section built near LaGrange, Georgia. A detailed description of the mechanical behavior of the test section before, during and after construction provides critically needed understanding of the internal behavior and macro-scale performance of this pavement structure. Given the critical role of the unbound aggregate base and its proximity to the surface, a new field test was developed to characterize the stress-dependent stiffness of the as-built layer. A complementary numerical study that incorporates state-of-the-art concepts in constitutive modeling of unbound aggregates is used to analyze experimental results and to develop preliminary guidelines for inverted base pavement design. Simulation results show that an inverted pavement can deliver superior rutting resistance compared to a conventional flexible pavement structure with the same fatigue life. Furthermore, results show that an inverted base pavement structure can exceed the structural capacity of conventional flexible pavement designs for three typical road types both in rutting and fatigue while saving up to 40% of the initial construction costs.

Epithelial to mesenchymal transition (EMT), the transdifferentation of an epithelial cell into a mesenchymal fibroblast, is a cellular process necessary for embryonic development and wound healing. However, uncontrolled EMT can result in accumulation of myofibroblasts and excessive deposition of ECM, contributing to the pathological progression of fibrotic diseases such as pulmonary fibrosis. The ability to control EMT is important for development of novel therapeutics for fibrotic pathologies and for designing novel biomaterials for tissue engineering applications seeking to promote EMT for development of complex tissues. EMT is a highly orchestrated process involving the integration of biochemical signals from specific integrin-mediated interactions with extracellular matrix (ECM) proteins and soluble growth factors such as TGFβ. TGFβ, a potent inducer of EMT, is activated via cell contraction-mediated mechanical release of the growth factor from a macromolecular latency complex. Thus TGFβ activity and subsequent EMT may be influenced by the biochemical and biophysical state of the surrounding ECM. Based on these knowns, it was hypothesized that both changes in integrin engagement and increases in substrate rigidity would modulate EMT due to changes in epithelial cell contraction and TGFβ activation. Here we show that integrin-specific interactions with fibronectin (Fn) fragments displaying both the RGD and PHSRN binding sites facilitate cell binding through α5β1 and α3β1 integrins, and lead to maintenance of epithelial phenotype, while Fn fragments displaying only the RGD site facilitate cell binding through αv integrins and lead to EMT. An in depth investigation into α3β1 binding to Fn fragments indicates that binding is dependent on both the presence and orientation of the PHSRN site. Studies investigating the contribution of ECM stiffening on EMT responses show that increasingly rigid Fn substrates are sufficient to induce spontaneous EMT. Analysis of TGFβ-responsive genes implicate TGFβ-expression, activation or signaling as a mechanism for the observed EMT responses. Together these results suggest that the ECM micromechanical environment is a significant contributor to the onset of EMT responses and provide insights into the design of biomaterial-based microenvironments for the control of epithelial cell phenotype.

<p>In this work the influence of Under Sleeper Pads on the dynamic forces on a railway track is investigated. A special interest is devoted to the effect of using Under Sleeper Pads in a railway track with changing vertical stiffness. The contact force between wheel and rail and the ballast contact forces are examined. For the investigation a finite element model with the length of thirty sleepers is created and calculations are performed with the software LS-DYNA. Three different cases of varying vertical track stiffness are studied: the transition from an embankment to a bridge, a randomly varying track stiffness along the railway track and hanging sleepers.</p>

<p> The aim of this thesis was to evaluate the use of dynamic excitation as a method for stiffness and strength grading of wet side boards of narrow dimensions. The need for such an investigation has previously been identified in an ongoing research project in which the possibility to use side boards as lamellae in wet glued glulam beams is investigated.</p><p>     The assessment of the dynamic excitation method was carried out by means of experimental work and measurements.. The approach was quantitative in the sense that the data was collected through experiments performed on a rather large population and that the results were analyzed using statistical methods.</p><p>     To investigate the effect of moisture content on stiffness of narrow dimension, side boards, the stiffness was measured in three states:</p><ul><li>- in wet state, before splitting the boards,</li><li>- in wet state, after splitting the boards, and </li><li>- in dry state (splitted boards).</li></ul><p>     The conclusion, after calculations and analysis of all the results, was that the natural frequency and stiffness of wet boards could, with a high degree of reliability, be predicted by use of the dynamic excitation method. There was a strong correlation in stiffness between wet state split boards and dry state split boards, with a coefficient of determination of 0.93.</p>

<p>The field of inverse problems is an area in applied mathematics that is of great importance in several scientific and industrial applications. Since an inverse problem is typically founded on non-linear and ill-posed models it is a very difficult problem to solve. To find a regularized solution it is crucial to have <em>a priori</em> information about the solution. Therefore, general theories are not sufficient considering new applications.</p><p>In this thesis we consider the inverse problem to determine the beam bending stiffness from measurements of the transverse dynamic displacement. Of special interest is to localize parts with reduced bending stiffness. Driven by requirements in the wood-industry it is not enough considering time-efficient algorithms, the models must also be adapted to manage extremely short calculation times.</p><p>For the developing of efficient methods inverse problems based on the fourth order Euler-Bernoulli beam equation and the second order string equation are studied. Important results are the transformation of a nonlinear regularization problem to a linear one and a convex procedure for finding parts with reduced bending stiffness.</p>

Syfte – Syftet med examensarbetet är att minimera eller helt eliminera den utböjning som uppstår i Husqvarnas trimmer 536 LiR från handtag ner till motor och trimmerhuvud då en 5 kilos vikt hängs i handtaget. Metod – En konceptstudie har utförts på Husqvarnas trimmer 536 LiR för att undersöka och utvärdera hur problemet med trimmern ser ut i dagsläget och därifrån ta fram olika koncept för att minimera utböjningen. Arbetet har följt en Stage-Gate produktutvecklingsprocess med sju steg från att skriva kravspecifikation till att ta fram och testa prototyper. Koncept genererades med metoderna brainstorming och Reverse Engineering och sållades sedan med Pughs beslutsmatris. Finita elementmetoden användes för att utvärdera produkten och för att analysera vissa koncept. Avancerade prototyper skapades med selektiv lasersintring medan enklare modeller togs fram för hand. Prototyperna testades och jämfördes mot originalprodukten. Resultat – I detta arbete presenteras fem olika förslag för att minska utböjningen i trimmern; en förstyvning av plastkåporna på originalprodukten, tre tillbehör att fästa på den befintliga trimmern och ett förslag på materialbyte. Alla förslag gav positiva resultat med minskad utböjning, men Tillbehör 3 var det som visade sig minimera utböjningen mest och det enda som klarade av att möta syftet. Dock gav detta tillbehör för stort viktpåslag. Förstyvningen och materialbytet gav också bra resultat gällande utböjningen och ökade inte vikten för mycket. Implikationer – Om de förslag som presenteras här skulle implementeras och tas i produktion skulle det innebära vissa tillfälliga konsekvenser vid produktion av trimmern. Verktygen för plastdetaljerna skulle behöva ändras för förstyvningen och kanske också för materialbytet, medan det för de två första tillbehören skulle behöva tillverkas helt nya verktyg för formsprutning. För att implementera Tillbehör 3 skulle nya beräkningar behövas eftersom trimmerns egenfrekvens ändras. Det skulle dock vara billigare att tillverka detta tillbehör än de första två eftersom detta inte behöver formsprutas. Begränsningar – Flertalet av prototyperna framtagna i detta projekt är tillverkade med selektiv lasersintring och inte med formsprutning, som är tillverkningsmetoden för de nuvarande plastkåporna. Detta innebär att hållfasthetsegenskaperna inte är desamma som de skulle bli vid verklig produktion. Dessutom är materialen som används glasfiberfyllda, vilket innebär att hållfastheten kommer att påverkas ytterligare beroende på tillverkningsmetod. Glasfibrernas positioner och riktningar blir olika vid olika processer vilket ger upphov till olika egenskaper. / Purpose – The purpose of this thesis is to minimize or completely eliminate the flexure in Husqvarna’s brush cutter 536 LiR from the handle down to the engine and the blade when a weight of 5 kilogrammes is hanged from the handle. Method – A concept study has been made on Husqvarna’s brush cutter 536 LiR to examine and evaluate what the problem with the model is and from that to develop different concepts to minimize the flexure. The work has followed a Stage-Gate product development process with seven steps from defining the specification of requirements to developing and testing prototypes. Ideas of concepts were generated using the methods brainstorming and Reverse Engineering and were later sifted using Pugh’s matrix. Finite Element Analysis was used to evaluate the product and to analyse certain concepts. Advanced prototypes were created using selective laser-sintering whilst simpler models were created by hand. The prototypes were tested and compared to the original product. Findings – This project presents five different suggestions on how to minimize the flexure in the brush cutter; one stiffer version of the original plastic casings, three accessories to attach to the original product and a proposal for a change of material. All these suggestions gave positive results with a lesser flexure, but the third accessory, Tillbehör 3, was the one that showed the smallest flexure and the only one able to fulfil the purpose of the study. The weight of the accessory was, however, too big. The stiffer version of the original casings and the change of material also showed good results regarding flexure and did not increase the weight too much. Implications – If any of the proposals of this project were to be implemented in production, it would cause certain temporary consequences for the production of the brush cutter. The tools for the plastic components of the current brush cutter would need some changing in order to manufacture the stiffer version of the mouldings or to produce the different material, whilst for the first two accessories completely new tools for injection moulding would have to be developed. To implement the third accessory, new calculations would be needed since the eigenfrequency of the brush cutter would be changed. It would, however, be cheaper to produce this accessory compared to the first two, since the third one does not need to be manufactured using injection moulding. Limitations – Most of the prototypes in this work is manufactured using selective laser sintering and not injection moulding, which is the manufacturing method of the current plastic casings. This means that the properties of the materials will be different than they would be if they were manufactured for production. Furthermore the materials used in the casings of the brush cutter are filled to certain degree with glass fibres, meaning that the material properties will be even more different depending on manufacturing method. The position and direction of the glass fibres will be different depending on which process is used, giving different mechanical properties.

While amputee gait has been studied in great detail, the influence of prosthetic foot sagittal and coronal plane stiffness on amputee walking biomechanics is not well understood. In order to investigate the effects of sagittal and coronal plane foot stiffness on amputee walking, a framework for manipulating the stiffness of a prosthetic foot needs to be developed. The sagittal and coronal plane stiffness of a low profile carbon fiber prosthetic foot was manipulated through coupling with selective-laser-sintered prosthetic ankles. The carbon fiber foot provided an underlying non-linear stiffness profile while the ankle modified the overall stiffness of the ankle-foot combination. A design of experiments was performed to determine the effect of four prosthetic ankle dimensions (keel thickness, keel width, space between the ankle top and bottom faces, and the location of the pyramid connection) on ankle-foot sagittal and coronal plane stiffness. Ankles were manufactured using selective laser sintering and statically tested to determine stiffness. Two of the dimensions, space between the ankle top and bottom faces and the location of the pyramid connection, were found to have the largest influence on both sagittal and coronal plane stiffness. A third dimension, keel thickness, influenced only coronal plane stiffness. A number of prosthetic ankle-foot combinations were created that encompassed a range of sagittal and coronal plane stiffness levels that were lower than that of the low profile carbon fiber foot alone. To further test the effectiveness of the framework to manipulate sagittal and coronal plane stiffness, two ankle-foot combinations, one stiffer than the other in the sagittal and coronal planes, were used in a case study analyzing amputee walking biomechanics. Differences in stiffness were large enough to cause noticeable changes in amputee kinematics and kinetics during turning and straight-line walking. Future work will expand the range of ankle-foot stiffness levels that can be created using this framework. The framework will then be used to create ankle-foot combinations to investigate the effect of sagittal and coronal plane stiffness on gait mechanics in a large sample of unilateral transtibial amputees. / text

Evaluation of the effect of particle cementation on the stiffness of uniform sand was carried out by measuring compression wave velocities (VP) and shear wave velocities (VS) on both clean and artificially cemented specimens. Piezoelectric transducers (PT) were used to perform the majority of the measurements. Shear wave velocity (VS), shear moduli (G) and material damping ratio (D) of clean and artificially cemented specimens were also determined using resonant column (RC) testing. Linear (shearing strains ≤ 0.001%) and nonlinear (shearing strains > 0.001%) behavior of the specimens were evaluated in the resonant column tests. The sand selected for this investigation is commonly known as Hickory sand, from the Hickory formation, western Llano uplift, Texas. This material was selected for its grain geometry and gradation; it consists of uniformly graded sand with rounded particles. The sand specimens were artificially cemented with a solution of hydrated sodium silicate and water. Sodium silicate is an alkaline compound obtained from the reaction of sodium hydroxide and silica. All artificially cemented specimens and uncemented hickory sand specimens were formed by pluviation through air. The microstructure of the specimens was visually assessed with images obtained from both optical and scanning electron (SEM) microscopes. These images confirmed that the procedure used to form artificially cemented specimens provides cementation around the contacts while some grain-to-grain contact appears to be preserved. Seismic and drained strength measurements on Hickory sand specimens were obtained from different cement concentrations and compared with results from clean sand specimens. Among the findings of this investigation are: (1) the procedure to artificially cement sand specimens in the laboratory was successful, (2) the slopes (nP and nS) obtained from the relationships between compression and shear wave velocities with effective isotropic confining pressure in log-log scale decrease as the cement content increases, and (3) as increasing amounts of cement are added to the sand particles, the nonlinearity of the specimens increases up to certain amount of cement, after which the nonlinearity of the specimen decreases and tends towards rock-like behavior. / text

A multitude of biophysical signals, including chemical, mechanical, and contact guidance cues, are embedded within the extracellular matrix (ECM) to dictate cell behavior and determine cell fate. To understand the complexity of the cell-matrix interaction and how changes to the ECM contribute to the development of tissues or diseases, three-dimensional (3D), culture systems that can decouple the effects of these cues on cell behavior are required. This dissertation describes the development and characterization of approaches based on multiphoton excitation (MPE) to control the chemical, mechanical, and topographical presentation of micro-3D-printed (μ-3DP) protein hydrogels independently. Protein hydrogels were chemically functionalized via the MPE-induced conjugation of benzophenone-biotin without altering the physical properties of the matrix. Complex, immobilized patterns and chemical gradients were generated within protein hydrogels with a high degree of spatial resolution in all axes. Hydrogel surfaces were also labeled with adhesive moieties to promote localized Schwann cell adhesion and polarization. Laser shrinking, a method based on MPE to manipulate the topographical and mechanical presentation of protein hydrogels after fabrication, is also presented. Topographical features on an originally flat substrate are created with depths approaching 6 μm. The Young’s modulus of protein hydrogels can also be increased by 6-fold (~15 – ~90 kPa) using laser shrinking, and parameters can be adjusted to create continuous gradient profiles for studying durotaxis. At determined scan conditions, the two properties can be adjusted independently of each other. Most importantly, the physical properties of the hydrogels can be manipulated in situ to study the effects of dynamic changes to the substrates on cells. As a potential tool to monitor cellular responses to presented cues, fluorescent probes that detect nitric oxide are characterized. Collectively, these technologies represent a key advance in hydrogel tunability, as the platforms presented offer independent, dynamic, and spatiotemporal control of the chemical, mechanical, and topographical features of protein hydrogels. The introduced technologies expand the possibilities of protein hydrogels to clarify underlying factors of cell-matrix interactions that drive morphogenesis and pathogenesis, and are broadly applicable to a multitude of physiological systems. / text

Asphalt mixtures or asphalt concrete are used to pave about 93% of about 2.6 million miles paved roads and highways in the US. Asphalt concrete is a composite of aggregates and asphalt binder; asphalt binder works as a glue to bind the aggregate particles. The mechanical response of the asphalt binder is dependent on the time/rate of loading, temperature and age. An asphalt concrete mixture inherits most of these characteristics from the asphalt binder. Also the asphalt binder plays a critical role in providing the asphalt concrete the ability to resist tensile stresses and relaxing thermally induced stresses that can lead to fatigue and low temperature cracking, respectively. Hence, it is very important (but not sufficient) to ensure that asphalt binders used in the production of asphalt concrete are inherently resistant to cracking, rutting and other distresses that a pavement may undergo. Current binder specification (AASHTO M-320) to evaluate its fatigue cracking is based on the stiffness of the binder and not on its tensile strength. Also, measurements following current specifications are made on test specimens subjected to a uniaxial mode of loading that does not produce the same stress state in the binder as in the case of asphalt concrete. Another challenge in being able to produce binders with inherently superior performing characteristics is the fact that the asphalt binders produced in a refinery do not have a consistent chemical composition. The chemical composition of asphalt binder depends on the source and refining process of crude oil. There is a need to better quantify the tensile strength of asphalt binder and understand the relationship between the chemical composition of asphalt binders and its mechanical properties. The knowledge from this study can be used to engineer asphalt binders that have superior performance characteristics. The objective of this research was to quantify the tensile strength of asphalt binder, develop a metric for the tensile strength and identify the relationship between chemical composition and mechanical properties of asphalt binder. Laboratory tests were performed on binders of different grades using a poker chip geometry to simulate confined state by varying the film thickness, rate of loading and modes of loading. The chemical properties of asphalt binder were studied based on SARA fractionation. The findings from this research showed that the modified correspondence principles can unify and explain the rate and mode dependency of asphalt binder. This study also quantified the relationship between chemical composition, and rheological and mechanical properties of asphalt binder. Finally, a composite model was developed based on the individual properties of chemical fractions which could predict the dynamic modulus of the asphaltenes doped and resins doped binder. / text