Thesis (Ph. D.)--Oregon State University, 2006. / Blank pages 143 and 159 not microfilmed. Includes bibliographical references. Also available online (PDF file) by a subscription to the set or by purchasing the individual file.

Thesis (Ph. D.)--Oregon State University, 2006. / Blank pages 143 and 159 not microfilmed. Includes bibliographical references.

Orientador: Edson Rosa Pimentel / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-06T17:20:03Z (GMT). No. of bitstreams: 1 Almeida_FernandaMartinsde_M.pdf: 1290451 bytes, checksum: c386eaff7996cd60bd0cc59338f8718f (MD5) Previous issue date: 2006 / Resumo: Os tendões servem para realizar a transferência de força dos músculos para os ossos, sendo capazes de suportar altas forças de tensão. Muitos trabalhos descreveram alterações nas propriedades estruturais, bioquímicas e biomecânicas dos tendões de animais que foram submetidos a exercícios prolongados, porém pouco se sabe sobre o que ocorre no tendão que passa por um processo de alongamento, um procedimento bastante comum em academias e clínicas de Fisioterapia. Assim, nosso trabalho teve como objetivo avaliar os aspectos morfológicos e bioquímicos dos tendões de ratos submetidos ao alongamento três e cinco vezes por semana. Os ratos tiveram seus músculos alongados por um período de 30 segundos intercalados com 30 segundos de relaxamento, com 10 repetições, 3 e 5 vezes por semana durante 21 dias. Os tendões foram removidos e utilizados para os procedimentos de morfologia e de bioquímica. Os tendões também foram submetidos ao ensaio mecânico sob tração a fim de avaliar suas propriedades biomecânicas. Nos cortes corados com AT, na entese dos tendões dos grupos alongados, observou-se um aumento na quantidade de células com morfologia arredondada. Já na região próxima à entese pode ser observado metacromasia mais intensa nos grupos alongados. Observações feitas nos cortes corados com HE, nas regiões de tensão, mostraram que as células apresentaram-se mais alinhadas. Nos grupos alongados, em ambas regiões ocorreu aumento na quantidade de células. Nos cortes que foram submetidos à Reação de Von Kossa, observou-se uma região calcificada em todos os grupos, porém esta apresentou uma MEC mais densa nos tendões dos grupos alongados três e cinco vezes. Análise do gel de SDS-PAGE revelou uma maior quantidade de colágeno nos grupos alongados e a presença do componente de 65 kDa nas regiões de tensão e compressão em todos os grupos. A quantidade de proteínas, de glicosaminoglicanos e de hidroxiprolina também foi superior nos animais alongados. O gel de agarose revelou a presença de dermatam sulfato nas regiões de tensão e de compressão e de condroitim sulfato somente nesta última. Durante o ensaio mecânico, os tendões dos grupos alongados suportaram valores de tensão máxima superiores, com deslocamentos semelhantes, sugerindo que estes tendões são mais resistentes à ruptura. Esses resultados mostraram que o estímulo do alongamento acarretou modificações nas características estruturais, bioquímicas e biomecânicas, confirmando o caráter adaptativo do tendão em resposta à aplicação dos procedimentos de alongamento / Abstract: The tendons are structures that transmit forces from the muscles to the bone, and are capable of supporting high tensile strenghts. Many studies have shown alterations in tendons of animals submitted to strenuous exercises, however, just a little is known about what happens in tendons when they are under a stretching program, a common procedure in academies and phisiotherapy institutes. So our objective was to evaluate the morphological, biochemical and biomechanical aspects of tendons submitted to stretching exercises. Rats had their muscles stretched for a period of 30 seconds with 30 seconds of resting, with 10 repetitions, three and five times a week during 21 days. The tendons were used for morphological and biochemical procedures. They were also submitted to mechanical tensile strain test and their mechanical properties were evaluated. Analysis of AT stained sections of enthesis from stretched tendons, showed a high amount of rounded cells. In the region next to enthesis, which passes close to the calcaneous, the metachromasy was more intense in stretched groups. In the tension region, in the HE stained sections, it was found a larger alignment of the cells in the stretched group. In both regions occurred an increase on the amount of cells. In the sections submitted to Von Kossa reaction, was observed, a calcified region in all groups, but the extracellular matrix were denser in stretched ones. Analysis of SDS-Page showed a high amount of collagen and the presence of a polidisperse component of 65 kDa in the tension and compression regions in all groups. The amount of proteins, glycosaminoglycans and hydroxiproline was higher in the tendons of three and five times stretched groups. The agarose gel revealed the presence of dermatan sulfate in the tension and compression regions and chondroitin sulfate only in this last one. During the mechanical tensile strain test, the stretched tendon supported high values of maximum stress with the same strain, when compared to the control group, suggesting that the stretched tendons were more resistant to the failure. These results show that the stimulus of stretching leads to alterations in the structural, biochemical and biomechanical characteristics, confirming the adaptative character of tendons, in response to the application of stretching exercises / Mestrado / Biologia Celular / Mestre em Biologia Celular e Estrutural

M.Tech. (Chiropractic) / Aim of this study was to compare which treatment either Proprioceptive Neuromuscular Facilitation or chiropractic manipulation, whether by itself or in combination, was more effective and beneficial in the treatment of mechanical neck pain. The effectiveness was measured by the use of a Visual Analogue Scale, Vernon- Mior Neck Pain and Disability Index questionnaire and the measurement of cervical spine range of motion using an analogous cervical spine range of motion inclinometer (CROM). The questionnaire and the measurements were taken prior to the treatments at the first, fourth and seventh consultations.Thirty participants who met the inclusion criteria were randomly placed into three groups of equal size (10 participants each). Group one received a Proprioceptive Neuromuscular Facilitation (PNF) stretching protocol to the cervical spine. Group two received a chiropractic manipulation to the cervical spine. Group three received a combination treatment, first receiving chiropractic manipulation and then a PNF stretching protocol to the cervical spine. Participants were treated six times out of a total of seven sessions, over a maximum of a three week period.Subjective data was collected at the beginning of the first, fourth and seventh consultations. This was done by means of a Visual Analogue Scale and a Vernon-Mior Neck Pain and Disability Index Questionnaire, in order to assess pain and disability levels. Objective data was collected by means of measuring cervical spine range of motion using a cervical range of motion (CROM) inclinometer. Analysis of collected data was performed by a statistician from STATKON, a department of the University of Johannesburg.Clinically significant improvements in group 1, group 2 and group 3 were noted over the duration of the study with reference to pain and disability. Statistically significant changes were seen in all three groups with reference to pain and disability. Group 3 was shown to improve the most with regard to pain and disability. Group 1 had statistically significant improvement with regard to cervical range of motion in the ranges of left and right rotation. Group 2 experienced a decrease range of cervical motion with regard to extension. As the study consisted of a small group of participants further, more extensive studies are needed...

This study examined the short-term training effects of two volumes of a dynamic warm up performed 4 days per week over a 3 1/2-week period. A total of 25 Division III wrestlers volunteered for the study. Three participants either dropped out or were unable to attend post-testing, resulting in 22 total participants completing the study. Groups were divided into control, low volume, and high volume groups. All participants completed pre and poststudy performance tests including the standing long jump, proagility, start-stop-cut, and 30- meter sprint. The low and high volume training groups each performed the same dynamic warm up prior to each pre-season captain's practice. The control group did not participate in an organized warm up. The low volume group performed one set of each warm up exercise, and the high volume group performing two sets of each warm up exercise. Data analysis indicated significant increases in performance for the standing long jump (p = .011) and start-stop-cut (p = .000) measures among the entire sample population. However, there was no significant difference between the groups in these measures. No significant results were found either for the sample as a whole or between groups for the proagility and 30-meter measures. The increased performance of all groups, including the control group, fails to provide evidence for the effectiveness of training with either warm up volume. Further research is needed to address limitations of this study to determine effectiveness of various warm up volumes.

O Teste de Sentar e Alcançar (TSA) é o teste mais utilizado para avaliação de flexibilidade dos isquiotibiais, contudo, alguns fatores influenciam o seu resultado como o comportamento da coluna vertebral. Esta pesquisa objetiva determinar a contribuição da articulação do quadril, da coluna lombar e da coluna torácica na execução do TSA em jovens brasileiros. Foi realizado um estudo observacional descritivo com uma amostra composta por 195 rapazes de 18 a 19 anos. Para identificar a contribuição dos segmentos corporais no movimento de flexão do tronco, realizou-se juntamente com o TSA uma análise cinemática angular por meio de Fotogrametria. Para tanto, foi desenvolvido um protocolo de avaliação de ângulos de referência que foram transformados em percentuais de contribuição dos segmentos. Os ângulos e percentuais foram classificados em dois padrões de referência do TSA, os quais permitiram identificar que quanto melhor a classificação no TSA, maior a utilização do quadril e menor a utilização da coluna torácica. A coluna lombar manteve-se estável durante o movimento. Os ângulos e percentuais foram classificados também nas categorias de IMC, revelando que indivíduos obesos utilizam mais o quadril para realizar o movimento, pois não conseguem fazer compensação com a coluna torácica. Com base nos resultados, foi possível criar uma tabela de classificação dos ângulos e percentuais, que permitiu identificar compensações e padrões de movimento conforme a condição das musculaturas envolvidas. Pôde-se concluir que as contribuições médias da coluna torácica, da coluna lombar e do quadril na realização do TSA são respectivamente: 46,014%, 12,676%, 41,309%. Entretanto, a medida do TSA propriamente dita não permite esse detalhamento, pois seu resultado faz referência à flexão total do tronco juntamente com a flexão do quadril. Assim, recomenda-se que a avaliação da flexão da coluna torácica, da coluna lombar e do quadril seja realizada separadamente, por fotogrametria, com base no protocolo e parâmetros médios estabelecidos neste estudo, por meio da classificação de seus valores nas categorias propostas. / The Sit and Reach Test (SR) is the most widely used assessment of hamstrings flexibility, however, some factors can influence their results as a behavior of the spine. This research aims to determine the contribution of the hip joint, of the lumbar and thoracic spine in the execution of SR in young Brazilians. The descriptive observational study subjects were 195 young men from 18 to 19 years. To identify the contribution of body segments in flexion of the trunk it was evaluated the SR together with angular kinematic analysis through of Photogrammetry. To that end, we developed a protocol for evaluating reference angles that were transformed into percentage contribution of the segments. The angles and percentages were classified into two reference standards of the SR, which identified that the best ranking in SR, greater use of hip and lower utilization of the thoracic spine. The lumbar spine was stable during movement. The angles and percentages were also classified in the categories of BMI, revealing that obese people use more hip to perform the movement, because they cannot make compensation with thoracic spine. Based on the results, it was possible to create a table of classification of angles and percentages, which allowed for the identification and compensation movement patterns in accordance with the condition of the muscles involved. It can be concluded the contributions of the thoracic spine, lumbar spine and hip in performing the SR are respectively 46.014%, 12.676%, 41.309%. However, only the measure of the SR does not permit this detail because its result refers to the total trunk flexion and hip flexion. Thus, it is recommended that evaluation of flexion of the thoracic spine, lumbar spine and hip joint are performed separately by photogrammetry, based on the average parameters and protocol established in this study, through the classification of their values in the proposed categories.

O Teste de Sentar e Alcançar (TSA) é o teste mais utilizado para avaliação de flexibilidade dos isquiotibiais, contudo, alguns fatores influenciam o seu resultado como o comportamento da coluna vertebral. Esta pesquisa objetiva determinar a contribuição da articulação do quadril, da coluna lombar e da coluna torácica na execução do TSA em jovens brasileiros. Foi realizado um estudo observacional descritivo com uma amostra composta por 195 rapazes de 18 a 19 anos. Para identificar a contribuição dos segmentos corporais no movimento de flexão do tronco, realizou-se juntamente com o TSA uma análise cinemática angular por meio de Fotogrametria. Para tanto, foi desenvolvido um protocolo de avaliação de ângulos de referência que foram transformados em percentuais de contribuição dos segmentos. Os ângulos e percentuais foram classificados em dois padrões de referência do TSA, os quais permitiram identificar que quanto melhor a classificação no TSA, maior a utilização do quadril e menor a utilização da coluna torácica. A coluna lombar manteve-se estável durante o movimento. Os ângulos e percentuais foram classificados também nas categorias de IMC, revelando que indivíduos obesos utilizam mais o quadril para realizar o movimento, pois não conseguem fazer compensação com a coluna torácica. Com base nos resultados, foi possível criar uma tabela de classificação dos ângulos e percentuais, que permitiu identificar compensações e padrões de movimento conforme a condição das musculaturas envolvidas. Pôde-se concluir que as contribuições médias da coluna torácica, da coluna lombar e do quadril na realização do TSA são respectivamente: 46,014%, 12,676%, 41,309%. Entretanto, a medida do TSA propriamente dita não permite esse detalhamento, pois seu resultado faz referência à flexão total do tronco juntamente com a flexão do quadril. Assim, recomenda-se que a avaliação da flexão da coluna torácica, da coluna lombar e do quadril seja realizada separadamente, por fotogrametria, com base no protocolo e parâmetros médios estabelecidos neste estudo, por meio da classificação de seus valores nas categorias propostas. / The Sit and Reach Test (SR) is the most widely used assessment of hamstrings flexibility, however, some factors can influence their results as a behavior of the spine. This research aims to determine the contribution of the hip joint, of the lumbar and thoracic spine in the execution of SR in young Brazilians. The descriptive observational study subjects were 195 young men from 18 to 19 years. To identify the contribution of body segments in flexion of the trunk it was evaluated the SR together with angular kinematic analysis through of Photogrammetry. To that end, we developed a protocol for evaluating reference angles that were transformed into percentage contribution of the segments. The angles and percentages were classified into two reference standards of the SR, which identified that the best ranking in SR, greater use of hip and lower utilization of the thoracic spine. The lumbar spine was stable during movement. The angles and percentages were also classified in the categories of BMI, revealing that obese people use more hip to perform the movement, because they cannot make compensation with thoracic spine. Based on the results, it was possible to create a table of classification of angles and percentages, which allowed for the identification and compensation movement patterns in accordance with the condition of the muscles involved. It can be concluded the contributions of the thoracic spine, lumbar spine and hip in performing the SR are respectively 46.014%, 12.676%, 41.309%. However, only the measure of the SR does not permit this detail because its result refers to the total trunk flexion and hip flexion. Thus, it is recommended that evaluation of flexion of the thoracic spine, lumbar spine and hip joint are performed separately by photogrammetry, based on the average parameters and protocol established in this study, through the classification of their values in the proposed categories.

Les étirements sont aujourd'hui une pratique courante dans les milieux sportifs et de réadaptation. Ils sont habituellement recommandés dans le but de contribuer à la prévention des blessures (Willson et al. 1991 ;Pope et al. 2000), à l’amélioration de la performance sportive lorsque celle-ci nécessite une amplitude articulaire importante (Heyters, 1985 ;Hortobagyi et al. 1985) et à la récupération de la mobilité articulaire dans le cadre d’un programme de réadaptation (Magnusson et al. 1996b). Ces méthodes, qui se sont développées ces dernières années, font classiquement appel à l’étirement passif et aux étirements neurofacilités (PNF), présentés initialement par Kabat (1958). Plus récemment, un intérêt particulier a été porté au travail musculaire excentrique. Des études ont montré que celui-ci permettait également d’augmenter l’amplitude articulaire (Willson et al. 1991 ;Nelson et Bandy, 2004). <p><p>Dans une première partie de ce travail, il nous a semblé intéressant de comparer les effets de l’étirement passif à ceux des étirements PNF par "contracté-relâché" et "contracté de l’antagoniste". Si les modalités d'application de chaque étirement sont différentes, elles présentent un intérêt majeur. De fait, elles permettent de faire varier les conditions d'allongement des différentes structures du système myotendineux, en modulant l'activité volontaire des musculatures agoniste ou antagoniste. En effet, si l’étirement passif s'effectue sans activation volontaire, l’étirement par "contracté-relâché" consiste à faire précéder l'étirement passif, d'une contraction volontaire maximale isométrique de la musculature agoniste. L’étirement par "contracté de l’antagoniste" associe à l'allongement de la musculature agoniste, une contraction volontaire maximale de la musculature antagoniste. Si de nombreuses études s'intéressent encore actuellement à caractériser leurs effets respectifs, elles ont déjà permis de montrer que ceux-ci avaient au moins deux origines distinctes, l'une neurophysiologique et l'autre mécanique (Taylor et al. 1990 ;Hutton, 1993). Au plan neurophysiologique, il est bien accepté que ces méthodes induisent une modulation de l'activité réflexe tonique facilitant le relâchement musculaire et par conséquent l’amplitude articulaire (Guissard et al. 1988 ;2001). Il est également bien admis que l’importance de ces effets sur la musculature est variable selon la méthode employée (Guissard et Duchateau, 2006). Au plan mécanique, des études menées chez l’animal ont montré que l’étirement passif modifie les caractéristiques viscoélastiques des tissus (Taylor et al. 1990), et de fait favorise l’allongement des tissus myotendineux (McHugh et al. 1992). Chez le sujet humain, plusieurs expérimentations ont montré que l’étirement par "contracté-relâché" permet d’obtenir un allongement myotendineux et un gain d’amplitude articulaire plus important que par étirement passif (Moore et Hutton, 1980). D’autres études ont montré que l’étirement par "contracté de l’antagoniste" permet de majorer encore les gains obtenus par l’étirement "contracté-relâché" (Osternig et al. 1990). Une première question posée dans ce travail est de savoir si la contribution des processus neurophysiologiques et mécaniques se traduit d’une manière spécifique sur le rapport de compliance des tissus de l’unité myotendineuse. Le développement de techniques d’investigation, telles que l’échographie, permet désormais d’observer le comportement de l’unité myotendineuse, d’une manière non invasive (Fukunaga et al. 1992 ;Herbert et Gandevia, 1995 ;Kuno et Fukunaga, 1995 ;Maganaris et al. 1998). Elle permet ainsi d’étudier les effets d’un étirement ou d’une contraction (Fukunaga et al. 1996) sur le rapport de compliance des structures musculaires et des tissus tendineux. <p> <p>S’il était intéressant d’étudier la spécificité de ces trois méthodes d’étirement classiques par rapport à leurs effets sur les tissus myotendineux, il nous paraissait pertinent d’observer celle d’un travail musculaire excentrique. En effet, la particularité de celui-ci est de soumettre l’unité myotendineuse préalablement activée, à un allongement. En réadaptation, le travail excentrique est généralement proposé dans le but d’améliorer plus rapidement la symptomatologie d’une tendinopathie (Stanish et al. 1986 ;Alfredson et al. 1998). Des études récentes ont indiqué qu’il peut également être proposé pour augmenter l'amplitude articulaire (Nelson et Bandy, 2004). Toutefois, ses effets sur les tissus myotendineux ne sont pas clairement définis dans la littérature. Chez l’animal, Heinemeier et al. (2007) ont comparé les effets d’un entraînement en contractions concentriques et excentriques sur les tissus de l’unité myotendineuse. Leurs résultats indiquent que si les tissus tendineux sont sensibles aux deux modes de contraction pour leurs effets favorisant la synthèse de collagène, les structures musculaires sont spécifiquement sensibles au mode excentrique. Chez le sujet humain, Crameri et al. (2004) ont observé une série de contractions excentriques d’intensité maximale augmente la synthèse de collagène au sein de l’ensemble des tissus de l’unité myotendineuse. Ces résultats montrent que les effets de ce travail ne se limitent pas aux tissus tendineux, tels que certains protocoles thérapeutiques le suggéraient, et que le tissu musculaire doit désormais être associé à la discussion des effets de ce travail musculaire. Dans ce contexte, la deuxième question que nous avons posée est de savoir si l’étirement de l’unité myotendineuse préalablement activée, telle que se caractérise une contraction excentrique, modifie le rapport de compliance des tissus myotendineux par rapport aux méthodes classiques. Autrement dit, si l’étirement de l’unité myotendineuse activée favorise spécifiquement l’allongement de l’un des tissus de l’unité myotendineuse. <p><p>Dans une troisième partie, il nous a paru intéressant d'étudier le comportement à l’étirement de l’unité myotendineuse, dont la compliance était modifiée suite à une désadaptation à long terme. L’hypertonie spastique, qui caractérise une majorité de sujets parétiques spastiques, est généralement caractérisée par une augmentation anormale des résistances opposées à l'étirement passif (Carey et Burghart, 1993). L’origine de ces résistances peut être attribuée à des adaptations tant neurophysiologiques (Pierrot-Deseilligny et Mazières, 1985) que mécaniques et structurelles des tissus de l'unité myotendineuse (Berger et al. 1984 ;Tardieu et al. 1989). Il n’existe pourtant pas encore dans la littérature de consensus les définissant clairement (Fridén et Lieber, 2003). En outre, l’étude récente de Lieber et Fridén (2002) a mis en évidence des modifications de l’architecture des muscles fléchisseurs du carpe. Contrairement à ce qui est généralement proposé au sujet de la longueur des fascicules spastiques (Tardieu et al. 1982), Lieber et Fridén (2002) n’ont pas mis en évidence de raccourcissement de ces derniers. Ainsi, l’hypothèse de Tardieu et al. (1982), selon laquelle le raccourcissement des fascicules est à l’origine de l’augmentation des résistances à l’allongement, ne peut plus être soutenue. Il nous paraissait ainsi intéressant d’associer, à l’observation du comportement des fascicules, celui des tissus tendineux, dans le but de déterminer si l’une de ces deux structures présente des caractéristiques particulières, susceptibles d’expliquer le développement plus important de ces résistances à l’allongement. La troisième question que nous avons posée dans ce travail est de savoir comment se caractérise, à l’étirement, le rapport de compliance des tissus musculaire et tendineux dans un contexte de spasticité. <p><p>Outre le fait que la réponse à ces différentes questions soit fondamentale pour mieux comprendre et spécifier la pratique des méthodes d’étirement, l’objectif de notre travail est non seulement de mettre en évidence le tissu dont l’allongement est spécifiquement favorisé par l’une ou l’autre méthode, mais également de contribuer à différencier celui qui limite l’étirement global du système myotendineux. Dans ce contexte, il sera intéressant de pouvoir recommander la pratique d’une méthode particulière, en fonction des besoins spécifiques de chacun. <p> / Doctorat en Sciences de la motricité / info:eu-repo/semantics/nonPublished