The motivation for this work has been to develop a web platform for a game concept where the underlying code acts to enable users to customize their game markers and place orders for them. This report deals with the creation of this website that in itself is not a webstore in classical terms but rather offers a process of it’s own for users to pick the looks of a product, place it in a cart and pay for it. The website communicates with an external payment processor that has been implemented. The process also includes prepatory work and the handeling of graphics and both client and server-side coding. The report covers the programming of the website and speculates in its application of usability standards from evaluating of user tests. / Motivationen för detta arbete har varit att ta fram en webbplattform för ett spelkoncept där kodningen ligger till grund för användare att personanpassa sina spelmarkörer och beställa dem. Denna rapport avhandlar skapandet av denna webbsida som i sig skiljer sig från en webbutik i klassisk bemärkelse och istället erbjuder en egen process för att välja utseende på varor, placera dem i en kundkorg och betala för dem. Webbsidan kommunicerar med en extern betalningslösning som är implementerad på sidan. I arbetet ingår förutom förarbete även hanterandet av bildmaterial och både klient- och serverbaserad kodning. Rapporten täcker framtagandet av tekniken för webbsidan och spekulerar i dess anpassning till användarriktlinjer och olika användartyper efter utvärderingar baserade på genomförda tester.

Magister Commercii - MCom / This study investigates the financial, marketing as well as technological feasibility of turning the current University of Western Cape (UWC) Student ID Card into a more featured debit card called the "Hoop Kaarf'. This debit card will offer the functionality of access to secure UWC areas, the purchase and use of photocopying credits, the purchase of printing credits, taking out library books; and identification, as the old student card does. It will also have the added functionality of replacing cash when paying for vending machines, cafés, takeaways, shops, supermarkets and withdrawing money from ATMs, at UWC, within South Africa and outside the country.

In thesis, we propose a structured light vision system equipped with multi-cameras and multi-laser emitters for object height measurement or 3D reconstruction. The proposed method offers a better accuracy performance over a single camera system. The structured light method may fail the interference of reflection and scattering of light. We use U-Net to extract the laser region, obtain the laser stripe center after erosion and dilation, and finally reconstruct the point cloud corresponding to the laser stripe. Our experiments demonstrate that our structured light system with the U-Net can perform effectively and robustly in a complex environment.

Thesis (PhD (Agric)) -- Stellenbosch University, 2003. / ENGLISH ABSTRACT: Stripe (yellow) rust of wheat, caused by Puccinia striiformis f.sp. tritici, was first detected as a single introduction into South Africa in 1996. Two additional pathotypes have since been identified. Control of the disease may be achieved by use of genetic adult plant resistance (APR) as is present in the local cultivar 'Kariega'. The aim of this project was to understand the genetic basis of the APR in 'Kariega' to facilitate breeding of new varieties with genetic resistance to stripe rust. A partial linkage map of a 'Kariega X Avocet S' doubled haploid population covering all 21 wheat chromosomes was generated using 208 DNA markers, viz, 62 SSR, 133 AFLP, 3 RGA and 10 SRAP markers, and 4 alternative loci. The different marker techniques detected varying polymorphism, viz, overall SSR: 46%, AFLP: 7%, SRAP: 6% and RGA: 9%, and the markers produced low levels of missing data (4%) and segregation distortion (5%). A significant feature of the linkage map was the low polymorphism found in the D genome, viz, 19% of all mapped DNA markers, 11% of all AFLP markers and 30% of the total genome map distance. A region exhibiting significant segregation distortion was mapped to chromosome 4A and a seedling resistance gene for stem rust (Puccinia graminis f.sp . tritici), Sr26, mapped to chromosome 6A close to three SSR markers. The leaf tip necrosis gene, Ltn, which was also segregating in the population, mapped to chromosome 7D. Protocols for SRAP and RGA were optimised, and SRAP marker use in wheat genetic linkage studies is reported for the first time. The linkage map was used together with growth chamber and replicated field disease scores for QTL mapping. Chromosomes showing statistically significant QTL effects were then targeted with supplementary SSR markers for higher resolution mapping. The quality of disease resistance phenotypic data was confirmed by correlation analysis between the different scorers for reaction type (0.799±0.023) and for transformed percentage leaf area infected (0.942±0.007). Major QTL were consistently identified on chromosome 7D (explaining some 25-48% of the variation) and on chromosome 2B (21-46%) using transformed percentage leaf area infected and transformed reaction type scores (early and final) with interval mapping and modified interval mapping techniques. Both chromosomal regions have previously been identified in other studies and the 7D QTL is thought likely to be the previously mapped APR gene Yr 18. Minor QTL were identified on chromosomes lA and 4A with the QTL on 4A being more prominent at the early field scoring for both score types. A QTL evidently originating from 'Avocet S' was detected under growth chamber conditions but was not detected in the field, suggesting genotype-environment interaction and highlighting the need for modifications of growth chamber conditions to better simulate conditions in the field. The genetic basis of the APR to stripe rust exhibited by 'Kariega' was established by mapping of QTL controlling this trait. The linkage map constructed will be a valuable resource for future genetic studies and provides a facility for mapping other polymorphic traits in the parents of this population with a considerable saving in costs. / AFRIKAANSE OPSOMMING: Streep of geelroes van koring word veroorsaak deur Puccinia striiformis f. sp tritici, en is die eerste keer in 1996 in Suid-Afrika na introduksie van 'n enkele patotipe waargeneem. Twee verdere patotipes is sedertdien in Suid-Afrika gei"dentifiseer. Beheer van die siekte word veral moontlik gemaak deur die gebruik van genetiese volwasseplantweerstand soos gei"dentifiseer in die plaaslike kultivar 'Kariega'. Die doel van hierdie studie was om die genetiese grondslag van die streeproesweerstand te ontrafel ten einde die teling van nuwe bestande kultivars moontlik te maak. 'n Verdubbelde haplo1ede populasie uit die kruising 'Kariega X Avocet S' is aangewend om 'n gedeeltelike koppelingskaart vir die volle stel van 21 koring chromosome saam te stel. Die kaart het uit 208 DNA merkers, nl., 62 SSR, 133 AFLP, 3 RGA, 10 SRAP merkers en 4 ander lokusse bestaan. Totale polimorfisme wat deur die verskillende merkersisteme opgespoor is, was as volg: SSR: 46%, RGA: 9%, AFLP: 7% en SRAP: 6%. Die mate van ontbrekende data was gering (4%) asook die mate van segregasie distorsie (5%) van 'n enkele geval wat op chromosoom 4A gekarteer is. 'n Prominente kenmerk van die koppelingskaart is die relatiewe gebrek aan polimorfiese merkers op die D-genoom, nl., slegs 19% van alle DNA merkers en 11% van alle AFLP merkers wat slegs 30% van die totale genoom kaartafstand bestaan het. Die stamroes (Puccinia graminis f. sp. tritici) saailingweerstandsgeen, Sr26, karteer op chromosoom 6A naby drie SSR merkers. Die geen vir blaartipnekrose, Ltn, karteer op chromosoom 7D. Protokolle vir SRAP en RGA merkers is ge-optimiseer en gebruik van SRAP merkers in koppelings-analise word vir die eerste keer in koring gerapporteer. Die koppelingskaart is in kombinasie met groeikamerdata en gerepliseerde veldproefdata gebruik om die gene (QTL) vir volwasseplant streeproesweerstand te karteer. Chromosome met statisties betekenisvolle QTL is met aanvullende SSR merkers geteiken om die resolusie van kartering verder te verhoog. Die kwaliteit van fenotipiese data, soos in die proewe aangeteken, is bevestig deur korrelasies te bereken tussen lesings geneem deur onafhanklike plantpataloe (0.799 ± 0.023 vir reaksietipe en 0.942 ± 0.007 vir getransformeerde persentasie blaaroppervlakte besmet). Hoofeffek QTL vir die twee maatstawwe van weerstand is deur middel van die metodes van interval QTL kartering en gemodifiseerde interval QTL kartering konsekwent op chromosome 7D (25-48% van variasie verklaar) en 2B (21-46% van variasie verklaar) ge"identifiseer. In vorige studies is aangetoon dat beide chromosome 7D en 2B QTL vir volwasseplant streeproesweerstand dra. Die 7D QTL is waarskynlik die weerstandsgeen, Yr 18. QTL met klein effekte op weerstand is op chromosome lA en 4A ge"identifiseer. Die effek van laasgenoemde geen was meer prominent in die velddata in die vroee datum van weerstandsbeoordeling. Een QTL, afkomstig van 'Avocet S', is slegs onder groeikamertoestande identifiseerbaar. Dit dui op moontlike genotipe-omgewing wisselwerking en beklemtoon die noodsaaklikheid om aanpassings te maak in groeikamertoestande vir beter simulasie van veldproeftoestande. Die genetiese grondslag van volwasseplantweerstand teen streeproes in die kultivar 'Kariega' is deur QTL kartering bepaal. Die 'Kariega X Avocet S' koppelingskaart kan as 'n waardevolle basis dien vir toekomstige genetiese ontledings van ander polimorfiese kenmerke in die populasie.

<p>Recently the society has been turning from the use of paper-based technologies to plastic cards in certain spheres of our life. With the emergence and proliferation of high technologies we cannot content with the security provided bypaper only. Therefore the society has chosen plastic to protect its information because it offers far more security based not only on human perception but also on machine-readable elements. </p><p>The number of plastic cards in circulation in different spheres of our everyday life increases constantly. They replace money, documents and allow easy and safe access to some services. In spite of its security the plastic card however is subjected to fraud. </p><p>Plastic card fraud results in significant losses for the various industries. Since the first appearance of plastic cards methods of committing fraud have changed dramatically. Now there is a wide range of high technologies at the disposal of criminals as well as card manufacturers. </p><p>Therefore we have put the great emphasize of this work on the analysis of the most common card technologies in the Plastic Card World, the magnetic stripe and the chip, existing crimes and main means of their committing. And we also have revealed the weak and strong sides of the prevention techniques, which are currently in use.</p>

Recently the society has been turning from the use of paper-based technologies to plastic cards in certain spheres of our life. With the emergence and proliferation of high technologies we cannot content with the security provided bypaper only. Therefore the society has chosen plastic to protect its information because it offers far more security based not only on human perception but also on machine-readable elements. The number of plastic cards in circulation in different spheres of our everyday life increases constantly. They replace money, documents and allow easy and safe access to some services. In spite of its security the plastic card however is subjected to fraud. Plastic card fraud results in significant losses for the various industries. Since the first appearance of plastic cards methods of committing fraud have changed dramatically. Now there is a wide range of high technologies at the disposal of criminals as well as card manufacturers. Therefore we have put the great emphasize of this work on the analysis of the most common card technologies in the Plastic Card World, the magnetic stripe and the chip, existing crimes and main means of their committing. And we also have revealed the weak and strong sides of the prevention techniques, which are currently in use.

L’adaptation des pathogènes à leurs hôtes et aux variations climatiques, particulièrement à la température est étudiée sur l’agent pathogène biotrophe obligatoire responsable de la rouille jaune du blé, Puccinia striiformis f. sp. tritici (Pst) au Moyen Orient. Cette étude s’est déroulée au Liban et en Syrie situés dans le berceau de la région de domestication du blé. Des gènes de résistance spécifique ont été postulés au stade plantule pour 87 lignées élites du programme d’amélioration de l’ICARDA,28 cultivars Libanais, et 23 landraces Libanaises en utilisant 11 pathotypes français disponibles à l’INRA-BIOGER. Un seul gène et une combinaison de gènes ont été postulés dans les lignées elites. Neuf gènes de résistance ont été identifiés dans les lignées élites ; plus de génotypes résistants figuraient parmi les lignées issues du programme d’amélioration. Les landraces sont les plus sensibles mais ont montré une ségrégation de réaction résistance parmi les plants sensibles.Pour la structuration de population pathogène du Liban et de la Syrie, un échantillonnage a été fait dans les deux pays sur du blé tendre, du blé dur et des repousses durant 2010-2011. Six isolats Libanais et 48 isolats Syriens ont été pathotypés avec une gamme de 43 hôtes différentiels. 275 échantillons ont été génotypés avec 20 marqueurs SSR. La population était clonale malgré avec la présence de l’hôte secondaire Berberis sp. dans la région, toutefois un nombre élevé de 50 MLG est observé était pour une population clonale. La présence de la race invasive PstS1/PstS2 caractérise cette région. Le profil de virulence Vr2, 6, 7, 9, 27 est le plus fréquent et typique du groupe génétique Méditerranéen (Bahri et al., 2009). La virulence Vr8 n’est pas fixée dans la population malgré sa présence dans la race invasive décrite depuis l’an 2000 (Milus et al., 2009). L’adaptation de la rouille jaune à la température a été décrite par Milus et al. (2009) et Mboup et al. (2012). Notre étude d’adaptation à la température a été faite sur un échantillon de 26 isolats provenant de zones froides et chaudes avec 4 isolats de référence. Nous avons testé deux paramètres d’agressivité, efficacité d’infection et période de latence sous quatre différents régimes de température (Chaud versus froid pour période de rosée et période d’incubation). Les isolats diffèrent pour leur réponse aux variations de température. Quelques isolats montrent une efficacité d’infection et une courte période de latence sous les différents régimes, d’autres sont efficaces au froid mais pas au chaud et vice versa. Pour l’efficacité d’infection, il n’y a pas d’adaptation mais par contre pour la période de latence on montre une adaptation à la température des isolats de la zone chaude ayant une efficacité d’infection. La température chaude de rosée a retardé la période de latence mais ce phénomène a été moins marqué pour les isolats d’origine chaude quand c’est incubé au chaud. Cette étude a montré que la population est clonale avec un haut nombre de pathotypes. Le germplasme n’est pas diversifié avec des gènes de résistance contre la rouille jaune. L’adaptation de l’agent de la rouille jaune à la température parmi les isolats testés a été décrite pour la période de latence pour les isolats provenant d’origine chaude. / The adaptation of fungal pathogen to its hosts and to the climate variation, in particular to the temperature, was investigated on wheat stripe (yellow) rust, caused by the biotroph fungus Puccinia striiformis f. sp. tritici (Pst) in the Middle East, focusing on Lebanon and Syria. This disease is a major problem for the crop in the region. Specific resistance genes were postulated in 138 wheat genotypes including elite lines, grown varieties and local landraces, using an array of 11 French pathotypes. Resistance gene diversity for yellow rust in wheat elite lines was higher than in current, commercial varieties grown in Lebanon, with nine Yr genes detected singly or in combination. Some varieties were resistant to all tested pathotypes and might provide interesting sources of resistance. Most of the Lebanese landraces were susceptible but also heterogeneous by their number of plants susceptible and resistant to a specific pathotype in a same landrace.A field survey was conducted in Lebanon and Syria in 2010-2011 and 275 Pst isolates were collected. The pathogen population was genotyped with 20 microsatellite markers and was found to be clonal, although the alternate host Berberis libanotica is present in the region. The dominant multilocus genotype shared similarity with the new invasive strain PstS1/PstS2 dispersed worldwide since 2000. The population was clonal with 10 pathotypes detected in Lebanon and Syria. 50 MLGs were detected considered high for clonal population. The virulence profiles combining Vr2, Vr6, Vr7, Vr9, and Vr27 are typical of the Mediterranean area according to group (Bahri et al., 2009) and corresponded to the worldwide invasive pathotype described since 2000 (Milus et al., 2009). The Vr8 was not fixed in this population, whereas this virulence is frequent in the Mediterranean genetic group (Bahri et al., 2009).Recently Pst strains have been described for adaptation to warm temperature (Milus et al., 2009; Mboup et al., 2012). The question of temperature adaptation in this study was whether the strains adapted to warm temperature are found in few clones of invasive strains or if they are selected in different pathogen genotypes locally under specific climate conditions. We selected 26 Pst isolates from the Middle East, 13 isolates from warm and 13 isolates from cold areas. We assessed their infection efficiency and latent period under four temperature regimes (high and warm temperature for the spore penetration phase, and high and warm temperature for the latency period). The isolates differed for the thermal aptitude for infection efficiency and latent period, but no clear relationship was established between the climate of the origin location of the isolate and its thermal aptitude. Some isolates were able to infect at high temperature but had long latency at high temperature and vice versa, some isolates had low infection efficiency and short latent period at high temperature, and few isolates were efficient either at high temperature or cold temperature for infection efficiency. Latency period showed pattern of local adaptation. Warm dew temperatures retarded sporulation, but this effect was far less marked for isolates from warm climates when incubated under warm conditions.This study provides details about probable effective yellow rust genes present in different genotypes and the prevalent pathotypes in the region. Moreover, the thermal aptitude for infection efficiency and latent period of some isolates under contrasting temperature will help us to build a better integrated disease management in the highlight of global warming.

Showing the barriers in the city, which inhibit a human in its movement along the landscape. Bunch of barriers at one place can signify a problem for a human to overcome. Marking these places is also showed below the prize map which illustrates from the economic point of view the problem exists at that place and the place is not that permeable as it should be. After marking the problematic spaces and their overlook in the real world there follows a choice of one place and its particular solution. Verifying the reasons why overcoming especially this place, what are the reasons for connecting it, analysing the areas on both sides, pointing out the place of connection and the way to solve the pedestrian bridge and finding the conceptual principle which is the support for the construction principle.

Master of Science / Department of Entomology / C. Michael Smith / The Russian wheat aphid Diuraphis noxia (Kurdjumov) is a serious pest of world cereal grain crops, primarily barley and wheat. A phenotypic characteristic of D. noxia feeding, leaf rolling, creates a leaf pseudo gall which protects aphids, making it difficult to treat infested plants with insecticides or biological control agents. Therefore, the use of D. noxia-resistant crops is a desirable aphid management tactic. Because of the development of virulent D. noxia biotypes, the identification of new sources of barley and wheat resistance is necessary. Virus-induced gene silencing (VIGS) utilizes the plant defense system to silence viruses in inoculated plants. The accumulation of virus RNA in plants triggers the defense system to silence sequences homologous to the introduced virus and sequences of interest from a plant are inserted into the virus and silenced along with the virus. The VIGS method was tested to determine the ability of barley stripe mosaic virus (BSMV) to serve as a VIGS vector in wheat plants containing the Dnx gene for resistance to D. noxia. Dnx leaves with silenced BSMV virus yielded D. noxia populations that were significantly no different from populations produced on healthy Dnx leaves. Thus, BSMV silencing does not interfere with Dnx resistance. Several different methods were examined to determine how best to confine aphids to the silenced leaf, and a modified plastic straw cage was chosen as the optimum cage type. Microarray and gene expression data were analyzed to select two NBS-LRR type disease resistance protein genes - TaAffx.104814.1.S1_at and TaAffx.28897.1.S1 - (NBS-LRR1 and NBSLRR2), in order to assess their role in Dnx resistance. NBS-LRR1 and NBSLRR2 were silenced by inoculating leaves of Dnx plants with barley stripe mosaic virus (BSMV) containing sequences of each gene. Controls included Dnx and Dn0 plants inoculated with BSMV and non-BSMV inoculated plants. Aphids were allowed to feed on control and treatment plants to assess aphid population and mean weight of aphids surviving at the end of the experiment. There were no differences among treatments based on aphid population, but there were significant differences the mean weights of aphids reared on several different treatments.

Les phases alpha'-Fe8N1-x et alpha''-Fe16N2 ont un fort potentiel d’application, en raison de leur anisotropie magnétocristalline uniaxiale et de leur grande aimantation à saturation. Cependant, les valeurs annoncées pour ces propriétés magnétiques restent sujettes à discussion. Les recherches menées au cours de cette thèse de doctorat ont été initiées dans le but de clarifier cette situation. L’élaboration des échantillons a principalement consisté en l’implantation ionique d’azote dans des couches minces de fer alpha épitaxiées sur ZnSe/GaAs (001). Entre autres, les effets de la température de la cible et de la fluence sur la structure cristalline des échantillons ont été analysés par diffractométrie des rayons X. La présence d’une anisotropie magnétique perpendiculaire a été mise en évidence dans les couches minces contenant les phases alpha'-Fe8N1-x ou alpha''-Fe16N2. La constante d’anisotropie a été évaluée par magnétométrie à échantillon vibrant et résonance ferromagnétique. À l’occasion de ces recherches, des domaines en rubans faibles ont été observés par microscopie à force magnétique dans certaines couches minces en Fe-N. Ceux-ci sont particulièrement rectilignes et des dislocations coin se trouvent au sein de leur structure périodique. Des études ont alors été réalisées dans le but de contrôler avec précision la réorientation des domaines en rubans et le déplacement des dislocations magnétiques, à l’aide d’un champ magnétique. / The alpha'-Fe8N1-x and alpha''-Fe16N2 phases have a high potential of application, because of their uniaxial magnetocrystalline anisotropy and their large saturation magnetization. However, the values announced for these magnetic properties remain a subject of discussion. The research conducted during this PhD thesis was initiated in order to clarify this situation. Sample making consisted mainly of nitrogen ion implantation into alpha-Fe thin films, epitaxially grown on ZnSe/GaAs (001). Among others, the effects of target temperature and fluence on the crystal structure of the samples were analyzed by X-ray diffractometry. The presence of a perpendicular magnetic anisotropy was demonstrated in the thin films containing the alpha'-Fe8N1-x and alpha''-Fe16N2 phases. The anisotropy constant was evaluated by vibrating sample magnetometry and ferromagnetic resonance. In this research, weak stripe domains were observed by magnetic force microscopy in some Fe-N thin films. These are particularly straight and edge dislocations are found within their periodic structure. Studies were then carried out to precisely control the reorientation of the stripe domains and the displacement of the magnetic dislocations, using a magnetic field.