Thesis (PhD (Agric))--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: During a survey in the greater Cape Flats Heterodera schachtii was found to be widespread on cauliflower, Brussels sprouts, beetroot and cabbage. The numbers present were above two eggs and juveniles per gram of soil, generally regarded as the economic threshhold level of infestation and requiring control. The damage potential of H schachtii on vegetables, as well as the ability of certain weeds to serve as a source of infection on subsequent crop plantings was studied under greenhouse conditions and resulted in a reduction of yield and root weight of crops. Population densities of H schachtii increased significantly under favourable hosts like cabbage where densities of 198 eggs and juveniles per gram of soil were reached. The most commonly occurring weeds maintained nematode development and increased their population densities. They can thus serve as alternative hosts in the absence of susceptible hosts and should be routinely controlled. The life cycle and biology of H schachtii was also studied. Penetration of plant tissue and subsequent development on vegetables, weeds and trap crops were observed. Penetration was successful on all crops tested reaching 37% and 52% at inoculum levels of 22 and 11 juveniles per gram of soil, respectively. Subsequent development of H schachtii on weeds and vegetables was similar, but in the case of cauliflower and black nightshade as hosts, their life cycle was shorter in comparison to other crops. The possible existence of varying susceptibility of crops to different populations of H schachtii was examined by comparing the rates of penetration in crops and reproduction of geographically isolated populations of H schachtii in the greater Cape Flats. When root penetration, virulence and juvenile emergence were examined, populations from Lynedoch and Philippi were distinct from the other populations. Subsequently, representative individuals of these populations were subjected to PCR-RFLP, but with these techniques real differences between the various populations could not be adequately detected. The environmental parameters such as soil texture, temperature and pH on H schachtii were investigated as to their influence on the root weight and yield of crops. Reductions in the yield of beetroot and cabbage were observed with soil temperatures ranging between 15 to 30°C. Migration and penetration of H schachtii juveniles declined with an increase in clay and silt content of the soil. Above a 34% silt and clay content of soil, no migration and penetration took place. Root penetration levels of 30% and higher were reached with pH varying between 4.5 and 7.4. This resulted in a significant reduction in yield of crops. Crop rotation is an essential component of non-chemical control. In the case of H schachtii, it required one host crop in four non-host rotational cycles to maintain the population of the nematode in the soilless than three eggs and juveniles per gram of soil. The inclusion of a trap crop reduced the population densities to below two eggs and juveniles per gram of soil. It therefore also forms an integral part of a control strategy. Solarization proved successful as a physical control method. Best results were obtained in summer with clear polyethylene which led to a 97% reduction of infective juveniles. This method can be applied during the late summer in the greater Cape Flats, just before the onset of winter. This may safeguard future spring plantings. The need for effective control strategies in order to reduce the numbers of H schachtii is of the utmost importance to ensure vegetable production in the future. Small-scale farmers should therefore be educated in this respect. / AFRIKAANSE OPSOMMING: Heterodera schachtii het wydverspreid in 'n opname in die groter Kaapse Vlakte voorgekom op beet, blomkool, Brusselse spruite en kopkool. Die nematode getalle by alle lokaliteite was bo die algemeen aanvaarbare ekonomiese drempelwaarde van twee eiers en larwes per gram grond wat beheer regverdig. Die skadepotensiaal van H schachtii op groente, sowel as die vermoë van sekere onkruide om as infeksie bronne te dien vir opvolgende gewasse, is in glashuise ondersoek en het tot 'n verlaging in opbrengs en wortelmassa by gashere gelei. Die populasie digthede van H schachtii het met die aanplant van geskikte gashere tot vlakke van 198 eiers en larvae per gram grond gestyg. Die mees algemeen voorkomende gasheeronkruide het nematode ontwikkeling in stand gehou en selfs tot 'n populasie verhoging gelei. Hierdie onkruide is 'n beperkende faktor vir die verbouing van groente aangesien die onkruide as alternatiewe gasheer kan dien in die afwesigheid van gashere en onkruidbeheer moet dus op 'n gereelde basis toegepas word. Die lewenssiklus en biologie van H schachtii is ondersoek deurdat die penetrasie van gasheer wortels en die daaropvolgende ontwikkeling op groente, onkruide en vanggewasse vergelyk is. Penetrasie, vyf dae na inokulasie, is met alle gashere verkry met 37% en 52% penetrasie met inokulum vlakke van 22 en 11 larwes per gram grond onderskeidelik. Daaropvolgende ontwikkeling van H schachtii was soortgelyk op groente en onkruide, maar blomkool en nastergal het as gashere 'n verkorte lewenssiklus tot gevolg gehad. Die moontlikheid van verskille in die virulensie van H schachtii is ondersoek deur die penetrasie van gewasse en reproduksie vlakke van nematodes van nege verskillende geografies geskeide populasies in the groter Kaapse Vlakte te vergelyk. Die Lynedoch en Philippi populasies het onderskeibare resultate gelewer ten opsigte van die populasies uit die ander lokaliteite, maar geen verskille kon met PKR-RFLP aangetoon word nie. Die invloed van omgewings parameters, grondtekstuur, temperatuur en pH, is op H schachtii ondersoek ten opsigte van opbrengste en wortelmassa van gewasse. Grondtemperature tussen 15°C - 30°C het tot die grootste daling in opbrengs gelei op kopkool en beet. Migrasie en penetrasie het afgeneem met 'n toename in klei en slik inhoud tot en met 'n klei en slik inhoud van 34%, waarna geen penetrasie en migrasie voorgekom het nie. Wortelpenetrasie van 30% en hoër het voorgekom by pH vlakke van tussen 4.5 - 7.4 met die gepaardgaande verlaging in opbrengs van gewasse. Afwisseling van gewasse is 'n essensiële metode van nie-chemiese beheer van nematode getalle in die grond. Die mees optimale rotasie ten opsigte van H schachtii beheer is met die aanplanting van een gasheer gewas in vier gewas aanplantings verkry. Die insluiting van 'n vanggewas in die gewas rotasie siklus het die nematode populasievlakke tot onder twee per gram grond laat daal. Solarisasie is suksesvol uitgevoer met deurskynende poli-etileen in die groter Kaapse Vlakte gedurende die somer met gevolglik 'n 97% vermindering van die getalle infektiewe nematodes. Effektiewe beheermaatreëls ten opsigte van H schachtii moet in die groter Kaapse Vlakte ingestel word om groente-produksie in hierdie gebied te verseker. Kleinboere moet in hierdie tegnieke opgelei word.

Field surveys were conducted in the Red River Valley (RRV) of North Dakota and Minnesota during 2016 and 2017 to determine the incidence, abundance, and distribution of plant-parasitic nematodes (PPNs) on sugarbeet. Seventy-two and 65 % of the fields surveyed were positive for PPNs in 2016 and 2017, respectively. The major genera of PPNs identified from sugarbeet production fields were Heterodera, Helicotylenchus, Tylenchorhynchus, Paratylenchus, Pratylenchus, Paratrichodorus, Hoplolaimus, and Xiphinema. Eight of PPNs were identified at the species level using species-specific PCR assays, and sequencing of the ribosomal rDNA gene. Stubby-root nematode, Paratrichodorus allius, is one of the important nematode pests for sugarbeet production worldwide. An experiment was conducted to determine the host status of sugarbeet and their rotational crops for P. allius under greenhouse conditions. The results from two experiments indicated sugarbeet and most rotational crops support the reproduction of P. allius. / Sugarbeet Research and Education Board (Minn.) / Sugarbeet Research and Education Board (N.D.) / American Crystal Sugar Company

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desaharifikacije melase i sprečavanje eventualnih nepoželjnih promena pri skladi&scaron;tenju melase. Stoga je predmet istraživanja ove disertacije redukovanje sadržaja nesaharoznih jedinjenja u melasi &scaron;ećerne repe upotrebom prirodnih adsorbenata mineralnog (različiti tipovi bentonita) i celuloznog (celuloza različitog stepena čistoće i modifikovani ekstrahovani rezanci &scaron;ećerne repe) porekla. Variranjem pH vrednosti sredine, suve materije melase i koncentracije primenjenog adsorbenta ispitan je sinergistički efekat uslova tretmana na stepen redukcije sadržaja nesaharoznih jedinjenja u melasi. Efikasnost sprovedenog tretmana utvrđena je na osnovu promena parametara kvaliteta melase i to boje, mutnoće, sadržaja saharoze, sadržaja suve materije i sadržaja pepela. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="font-size:10.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Times New Roman&quot;">Na osnovu rezultata uočava se da je pozitivan uticaj kombinovanog dejstva upotrebljenih adsorbenata i uslova tretmana najizraženiji kod mutnoće i boje melase &scaron;to potvrđuje afinitet adsorbenata ka zadržavanju i/ili vezivanju bojenih materija. Takođe, navedeni pozitivan uticaj izražen je u manjoj ili većoj meri u zavisnosti od tipa primenjenog adsorbenta ali i uslova gde je uglavnom dominantan uticaj pH vrednosti </span></p><p><!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML/> <o:AllowPNG/> </o:OfficeDocumentSettings></xml><![endif]--><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves/> <w:TrackFormatting/> <w:DoNotShowRevisions/> <w:DoNotPrintRevisions/> <w:DoNotShowMarkup/> <w:DoNotShowComments/> <w:DoNotShowInsertionsAndDeletions/> <w:DoNotShowPropertyChanges/> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF/> <w:LidThemeOther>EN-US</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> <w:SplitPgBreakAndParaMark/> <w:EnableOpenTypeKerning/> <w:DontFlipMirrorIndents/> <w:OverrideTableStyleHps/> </w:Compatibility> <m:mathPr> <m:mathFont m:val="Cambria Math"/> <m:brkBin m:val="before"/> <m:brkBinSub m:val="&#45;-"/> <m:smallFrac m:val="off"/> <m:dispDef/> <m:lMargin m:val="0"/> <m:rMargin m:val="0"/> <m:defJc m:val="centerGroup"/> <m:wrapIndent m:val="1440"/> <m:intLim m:val="subSup"/> <m:naryLim m:val="undOvr"/> </m:mathPr></w:WordDocument></xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" DefUnhideWhenUsed="true" DefSemiHidden="true" DefQFormat="false" DefPriority="99" LatentStyleCount="267"> <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Normal"/> <w:LsdException Locked="false" Priority="9" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="heading 1"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 2"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 3"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 4"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 5"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 6"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 7"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 8"/> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 9"/> <w:LsdException Locked="false" Priority="39" Name="toc 1"/> <w:LsdException Locked="false" Priority="39" Name="toc 2"/> <w:LsdException Locked="false" Priority="39" Name="toc 3"/> <w:LsdException Locked="false" Priority="39" Name="toc 4"/> <w:LsdException Locked="false" Priority="39" Name="toc 5"/> <w:LsdException Locked="false" Priority="39" Name="toc 6"/> <w:LsdException Locked="false" Priority="39" Name="toc 7"/> <w:LsdException Locked=&qu / <p>&nbsp;</p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="font-size:10.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-fareast-font-family:Calibri;mso-bidi-font-family:&quot;Times New Roman&quot;">The application of different treatments aiming to reduce the content of non-sugar compounds in sugar beet molasses is a potential method for molasses quality enhancement, facilitation of molasses desugarization process and prevention of any undesirable changes during molasses storage. Therefore, the objective of this dissertation is to reduce the content of non-sugar compounds in sugar beet molasses by using natural adsorbents of mineral (different types of bentonite) and cellulosic (cellulose of different purity and modified sugar beet pulp) origin. By varying treatment conditions such as pH, molasses dry substance and the applied adsorbent concentration, the synergistic effect of the treatment conditions on the reduction efficiency of non-sugar compounds content in molasses was examined. The treatment effectiveness was determined based on changes in molasses quality parameters, namely color, turbidity, sucrose content, dry substance content and ash content.</span></p><p><!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML/> <o:AllowPNG/> </o:OfficeDocumentSettings></xml><![endif]--></p><p><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves/> <w:TrackFormatting/> <w:DoNotShowRevisions/> <w:DoNotPrintRevisions/> <w:DoNotShowMarkup/> <w:DoNotShowComments/> <w:DoNotShowInsertionsAndDeletions/> <w:DoNotShowPropertyChanges/> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF/> <w:LidThemeOther>EN-US</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> <w:SplitPgBreakAndParaMark/> <w:EnableOpenTypeKerning/> <w:DontFlipMirrorIndents/> <w:OverrideTableStyleHps/> </w:Compatibility> <m:mathPr> <m:mathFont m:val="Cambria Math"/> <m:brkBin m:val="before"/> <m:brkBinSub m:val="&#45;-"/> <m:smallFrac m:val="off"/> <m:dispDef/> <m:lMargin m:val="0"/> <m:rMargin m:val="0"/> <m:defJc m:val="centerGroup"/> <m:wrapIndent m:val="1440"/> <m:intLim m:val="subSup"/> <m:naryLim m:val="undOvr"/> </m:mathPr></w:WordDocument></xml><![endif]--></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="font-size:10.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-fareast-font-family:Calibri;mso-bidi-font-family:&quot;Times New Roman&quot;">According to the obtained results, the positive influence of the combined effect of applied adsorbents and treatment conditions is most pronounced for molasses quality parameters turbidity and colour, which confirms the applied adsorbents affinity towards coloured compounds retention or binding. Also, the stated positive influence is expressed to a greater or lesser extent depending on the type of adsorbent used as well as the conditions applied, where the influence of pH is mainly dominant.</span></p><p><!--[if gte mso 10]><style> /* Style Definitions */ table.MsoNormalTable{mso-style-name:"Table Normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-priority:99;mso-style-parent:"";mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin-top:0cm;mso-para-margin-right:0cm;mso-para-margin-bottom:10.0pt;mso-para-margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri","sans-serif";mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-ansi-language:EN-US;mso-fareast-language:EN-US;}</style><![endif]--></p><p><!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML/> <o:AllowPNG/> 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La betterave sucrière est une plante bisannuelle dont le besoin de vernalisation est absolu. Ce processus correspond à l’acquisition de l’aptitude à la montaison et à la floraison et résulte d’une exposition prolongée à de basses températures. La durée de froid requise pour induire la montaison puis la floraison varie suivant les génotypes et reflète leur tolérance à la montaison, qui constitue donc un caractère agronomique essentiel. Cette thèse visait à (i) mettre en évidence un éventuel contrôle épigénétique (méthylation ADN) de l‘induction de la montaison chez des génotypes de betterave sucrière résistants ou sensibles à la montaison, (ii) identifier les séquences ciblées par des remaniements de méthylation de l’ADN et d’expression associés, et (iii) caractériser certaines séquences candidates en vue de leur utilisation comme marqueurs de la montaison. Nos travaux ont montré que l’amplitude et la cinétique des variations de méthylation de l’ADN observées au cours de la vernalisation semblent être des éléments critiques de l’induction et de la tolérance à la montaison. Par une approche ciblée, des séquences dont la méthylation de l’ADN est remaniée ont été identifiées. L’implication dans la transition florale de deux BvRNMT (RNA METHYLTRANSFERASES) et de la méthylation des ARN, tels que l’ARNm de BvFL1, un répresseur floral, a ainsi été mise en évidence chez la betterave sucrière. Enfin, grâce à une approche génomique, un réseau de gènes intégratif incluant la réponse à l’environnement, la signalisation hormonale et l’induction de la floraison a été identifié. La cinétique d’activation de ces gènes définirait le niveau de tolérance à la montaison des différents génotypes de betterave sucrière. / Sugar beet is a biennial plant with an absolute requirement of vernalization, corresponding to the acquisition of the competence to bolt and flower after a prolonged exposure to low temperatures. The cold duration needed to induce bolting and flowering varies depending on the genotypes, reflecting their bolting tolerance, which is an essential agronomic trait. This work aimed at (i) investigating a possible epigenetic control of bolting induction in bolting sensitive and bolting resistant sugar beet genotypes, (ii) identifying sequences targeted by DNA methylation and expression remodeling, and (iii) characterizing candidate sequences which could be used in marked-assisted selection for plant breeding. Our data suggest that the time course and amplitude of DNA methylation variations are critical points for the induction of sugar beet bolting and represent an epigenetic component of the genotypic bolting tolerance. In addition, we identified differentially methylated sequences exhibiting variations of gene-body DNA methylation and expression during cold exposure and/or between genotypes. Among them, two RNA METHYLCYTOSINE TRANSFERASES, in association with RNA methylation such as BvFL1 mRNA, a floral repressor, were shown to play a role in floral transition. Finally, using microarrays we identified an integrative network of genes including response to environment, phytohormone signalling and flowering induction. The activation kinetics of these genes could define the bolting tolerance level of sugar beet genotypes.

<p>Poznavanje genetičke divergentnosti predstavlja osnovni preduslov uspe&scaron;nog oplemenjivačkog programa. Pored poznavanja genetičke divergentnosti, u proizvodnji hibrida &scaron;ećerne repe je od izuzetne važnosti i poznavanje kombinacionih sposobnosti oplemenjivačkog materijala. Svako ukr&scaron;tanje inbred linija nema uvek za posledicu pojavu heterozisa, pa je stoga neophodno ispitati op&scaron;te i posebne kombinacione sposobnosti onih linija koje se planiraju koristiti kao roditeljske komponente hibrida. U ovom istraživanju ispitivane su kombinacione sposobnosti multigermnih opra&scaron;ivača, razvijenih u okviru četiri različita oplemenjivačka programa: programa oplemenjivanja &scaron;ećerne repe Instituta za ratarstvo i povrtarstvo, Novi Sad, i tri programa oplemenjivanja &scaron;ećerne repe istraživačkih stanica Ministarstva poljoprivrede SAD (Michigan, Salinas i Fort Collins). Opra&scaron;ivači su se razlikovali u stepenu homozigotnosti u zavisnosti od prisustva gena autofertilnosti, odnosno autosterilnosti. Kao testeri kori&scaron;ćene su dve citoplazmatski sterilne linije Instituta za ratarstvo i povrtarstvo, Novi Sad. Pored određivanja genetičke divergentnosti i kombinacionih sposobnosti cilj ovog istraživanja je bio utvrđivanje vrednosti kvantitativnih svojstava za najvažnija svojstva korena: masu korena, sadržaj &scaron;ećera, sadržaj suve materije, masu glave korena, obim korena, procenat iskori&scaron;ćenja, prinos kristalnog &scaron;ećera i na osnovu njih određivanje genetičke divergentnosti ispitivanih opra&scaron;ivača. Takođe na osnovu dobijenih vrednosti određen je efekat gena i način nasleđivanja za ispitivana kvantitativna svojstva.Opra&scaron;ivač FC220 se izdvojio kao potencijalno stabilan kombinator u<br />iii<br />obe godine istraživanja, beležeći pozitivne vrednosti op&scaron;tih kombinacionih sposobnosti za sva ispitivana svojstva. Sa druge strane opra&scaron;ivač EL53 je u obe godine istraživanja beležio negativne vrednosti op&scaron;tih kombinacionih sposobnosti za sva ispitivana svojstva. U pogledu načina nasleđivanja mase korena, obima korena i prinosa kristalnog &scaron;ećera superiornost su pokazali autofertilni polinatori u odnosu na populacije slobodne oplodnje. Veći stepen homozigotnosti i uniformnost F1 generacije doveli su do ispoljavanja efekta heterozisa kod hibridnih kombinacija gde su roditelji bili autofertilni polinatori. Na osnovu načina grupisanja multigermnih opra&scaron;ivača u zbirnom klasteru, potvrđena je negativna korelacija između mase korena i sadržaja &scaron;ećera. U okviru jedne grupe su se na&scaron;li opra&scaron;ivači sa velikom masom korena i nižim sadržajem &scaron;ećera, a u drugoj grupi sa malom masom korena i vi&scaron;im sadržajem &scaron;ećera. Analizom međusobnih odnosa multigermnih opra&scaron;ivača &scaron;ećerne repe pomoću SSR markera konstruisan je dendrogram u kome su opra&scaron;ivači podeljeni u četiri grupe, shodno centrima porekla iz kojih su dobijeni. Između genetičke udaljenosti određene pomoću podataka dobijenih SSR markerima i posebnih kombinacionih sposobnosti nisu ustanovljene korelacije.<br />Datum prihvatanja</p> / <p>Information about genetic diversity is basic requirement of every successful breeding program. Beside information about genetic diversity for development of sugar beet hybrids it is very important to know combining abilities of breeding material. Since not all crosses result with appearance of heterosis, it is necessary to test general and specific combining abilities of potential parental lines. In this research were used multigerm pollinators from four different breeding programs: sugar beet breeding program of Institute of Field and Vegetable Crops, Novi Sad, and three programs from different breeding station of the US Department of Agriculture (Michigan, Salinas and Fort Collins). Pollinators differed in the degree of homozygosity, depending on the presence of genes for autofertility or sterility. Testers used in this work were two cytoplasmic sterile lines of the Institute of Field and Vegetable Crops, Novi Sad. In addition to determining the genetic diversity and combining ability, objective of this study was to determine values of quantitative traits for sugar beet root traits: root weight, sugar content, dry matter content, root head weight, root circumference, extractable sugar content, crystal sugar yield and from them a genetic diversity of pollinators. Also on the basis of the obtained values it was determined gene effect and mode of inheritance of studied<br />vi<br />quantitative traits. Pollinator FC220 segregated as a potentially stable combiner, in both years, having positive values of general combining abilities for all traits. On the other hand pollinator EL53 in both years had negative values of general combining abilities for all traits. In terms of the mode of inheritance for root weight, root circumference and crystal sugar yield self-fertile pollinators showed superiority comparing to the population of open pollination. A higher level of homozygosity and uniformity of the F1 generation resulted in the expression of heterosis in hybrid combinations where the parents were self-fertile pollinators. Multigerm pollinators in aggregate cluster confirmed the negative correlation between root weight and sugar content. In one group were found pollinators with a large root mass and lower sugar content, while in the second group were pollinators with a small root mass and higher sugar content. Cluster analysis of multigerm sugar beet pollinators using SSR markers resulted in construction of dendrogram in which pollinators were divided into four groups, according to the centers of origin from which they were obtained. There was no correlation between genetic distance calculated from the data obtained by SSR markers and specific combining ability.</p>

Recent studies of labour have clearly established that the capitalist state is very involved in the recruitment, relocation and retention of migrant labour forces. Most of the literature tends to analyze migrant labour within the broader social, political and economic context of expanding capitalism. Consequently, studies tend to focus on how the use of migrant labour is profitable to capitalism because it is cheap and easy to exploit. Such studies, however, neglect the ways in which the state actually intervenes in the labour market in order to facilitate the flow of migrant workers to places of employment. Therefore, this thesis explores the relationship between the migration of labour, the state and the reserve army of labour through an analysis of the Native migrant work force in the sugar beet industry in southern Alberta.<p> Through the use of archival material, which includes various federal and provincial documents, annual reports of the Alberta Sugar Beet Growers' Association, newspapers and other materials, the circumstances underlying state intervention in the economy of the southern sugar beet industry became clear. While analyzing the structure of the sugar beet industry in southern Alberta, it was found that throughout much of the history of the sugar beet industry, farmers received low returns for their beet crops. Moreover, farmers also suffered financially from the high cost of machinery and, more recently, from the increased costs for fertilizer and chemical weed controls.<p> An examination of government documents on the FederalProvincial Agricultural Manpower Committee, whose mandate was to recruit workers and move them to areas of need in agricultural sectors throughout Canada, revealed that the federal part of the committee was represented by officials from the Department of Manpower and Immigration and, beginning in the early 1950s, officials from the Department of Indian Affairs, who represented Indians on reserves.<p> When the working conditions in sugar beet industry were examined, it was found that they were very poor for beet workers. In general, the weeding and hoeing of the sugar beets was difficult and the housing accommodations inadequate. Moreover, because of the low return on their beet crops and the high costs of machinery, fertilizer and weed control, the farmers had to keep the cost of labour as low as possible, which, meant paying low wages to beet workers. Moreover, it was found that throughout much the history of the sugar beet industry in southern Alberta, agricultural workers were unprotected by labour laws, which, was very conducive to reproducing conditions for cheap labour. Consequently, few wanted to work in the beet fields of southern Alberta if other employment could be found.<p> Prior to the 1950s the state recruited immigrant workers and even prisoners of war from internment camps to supply farmers with the needed labour for their beet crops. However, in the early 1950s unskilled immigrant labour could no longer be procured for beet work. It was at this time that the sugar beet industry, through the Federal-Provincial Agricultural Manpower Committee, turned to recruiting Natives, particulary northern Alberta and northern Saskatchewan reserve Indians, to perform their labour requirements. In order to maintain this needed work force, the state helped organize Native migratation to southern Alberta at the start of the beet season and also helped ensure that they stayed there for the duration of the needed period.

Recent studies of labour have clearly established that the capitalist state is very involved in the recruitment, relocation and retention of migrant labour forces. Most of the literature tends to analyze migrant labour within the broader social, political and economic context of expanding capitalism. Consequently, studies tend to focus on how the use of migrant labour is profitable to capitalism because it is cheap and easy to exploit. Such studies, however, neglect the ways in which the state actually intervenes in the labour market in order to facilitate the flow of migrant workers to places of employment. Therefore, this thesis explores the relationship between the migration of labour, the state and the reserve army of labour through an analysis of the Native migrant work force in the sugar beet industry in southern Alberta.<p> Through the use of archival material, which includes various federal and provincial documents, annual reports of the Alberta Sugar Beet Growers' Association, newspapers and other materials, the circumstances underlying state intervention in the economy of the southern sugar beet industry became clear. While analyzing the structure of the sugar beet industry in southern Alberta, it was found that throughout much of the history of the sugar beet industry, farmers received low returns for their beet crops. Moreover, farmers also suffered financially from the high cost of machinery and, more recently, from the increased costs for fertilizer and chemical weed controls.<p> An examination of government documents on the FederalProvincial Agricultural Manpower Committee, whose mandate was to recruit workers and move them to areas of need in agricultural sectors throughout Canada, revealed that the federal part of the committee was represented by officials from the Department of Manpower and Immigration and, beginning in the early 1950s, officials from the Department of Indian Affairs, who represented Indians on reserves.<p> When the working conditions in sugar beet industry were examined, it was found that they were very poor for beet workers. In general, the weeding and hoeing of the sugar beets was difficult and the housing accommodations inadequate. Moreover, because of the low return on their beet crops and the high costs of machinery, fertilizer and weed control, the farmers had to keep the cost of labour as low as possible, which, meant paying low wages to beet workers. Moreover, it was found that throughout much the history of the sugar beet industry in southern Alberta, agricultural workers were unprotected by labour laws, which, was very conducive to reproducing conditions for cheap labour. Consequently, few wanted to work in the beet fields of southern Alberta if other employment could be found.<p> Prior to the 1950s the state recruited immigrant workers and even prisoners of war from internment camps to supply farmers with the needed labour for their beet crops. However, in the early 1950s unskilled immigrant labour could no longer be procured for beet work. It was at this time that the sugar beet industry, through the Federal-Provincial Agricultural Manpower Committee, turned to recruiting Natives, particulary northern Alberta and northern Saskatchewan reserve Indians, to perform their labour requirements. In order to maintain this needed work force, the state helped organize Native migratation to southern Alberta at the start of the beet season and also helped ensure that they stayed there for the duration of the needed period.

In this study, bio-hydrogen generation potential of sugar-beet processing wastes (sugar-beet processing wastewater and beet-pulp) through dark fermentation was investigated. For this purpose, four different experimental set-ups were used. In the first set-up, sugar-beet processing wastewater was used along with four different cultures to investigate the effect of culture type on bio-hydrogen production. In addition, unseeded reactor was prepared to investigate bio-hydrogen production potential of indigenous microorganisms. The highest bio-hydrogen production yield (87.7 mL H2/g COD) was observed in the unseeded reactor. In the second set-up, beet-pulp was compared with sugar-beet processing wastewater in terms of bio-hydrogen generation potentials at an initial COD level of 4.5 g/L. In the third set-up, bio-hydrogen productivities of only beet-pulp and co-digestion of beet-pulp and sugar-beet processing wastewater at high COD values were investigated. The results of third set-up revealed that the reactor fed by 20 g/L COD beet-pulp provided the highest bio-hydrogen production yield (95.6 mL H2 /g COD). Finally, in the fourth set-up, the effects of five different pretreatment methods on solubilization of beet-pulp were investigated. Then, three out of five pretreatment methods were chosen to compare the corresponding bio-hydrogen productivities. Maximum bio-hydrogen production yield (115.6 mL H2/g COD) was observed in reactor which contained alkaline pretreated beet-pulp. Based on the results obtained in this study, it is postulated that, bio-hydrogen production from sugar-beet processing wastes by dark fermentation can not only enable waste minimization but also contribute to sustainability via valuable bio-based product formation from wastes, namely bio-hydrogen.

Biological hydrogen production through integration of dark and photo-fermentation by using biomass is a promising alternative for energy supply problems. The main purpose of this study was to investigate photobiological H2 production by the purple non-sulfur (PNS) bacteria Rb. capsulatus on dark fermentation effluent of sugar beet thick juice (DFESBTJ). Presence of NH4+ in effluents is an important parameter since NH4+ inhibit the nitrogenase enzyme activity. Therefore, the influence of different NH4+ concentrations in the DFESBTJ by removing using natural zeolite clinoptilolite on photofermentative H2 production were studied using Rb. capsulatus DSM1710 and Rb. capsulatus YO3 (hup-). Also, the effect of EtOH concentrations (between 6.25 and 200 mM) in the defined medium on H2 production were studied using both bacterial strains since EtOH is a possible by-product of dark fermentation process. The experiments were carried out in small scale bottle photobioreactors (PBRs) and outdoor panel PBR (4 L). H2 productivity of 1.12 mmol/Lc/h was attained over 15 days of operation for panel PBR. The results showed that the zeolite was effective in removing NH4+ from the DFESBTJ as its concentration decreased by 95% after treatment. In both bacterial strains, an increase in the maximum productivities and molar H2 yields was observed with the decrease in NH4+concentrations. There was no significant effect of EtOH on H2 production except the inhibition at 200 mM. The main conclusions were that both bacterial strains could effectively utilize the DFESBTJ for growth and H2 production, therefore facilitating the integration of the dark and photo-fermentation for sustainable biohydrogen production.