Global ETD Search

21	Design of a Dissolved Oxygen Optical Sensing Device for Cell Growth and Metabolism Monitoring in Bioreactors Rosa, Raelyn K. 04 1900 (has links) <p>An electro-optical sensor module was designed to monitor the level of dissolved oxygen (DO) using the method of frequency domain ﬂuoroscopy. Frequency domain ﬂuoroscopy is an optical method that detects the concentration of an analyte by indirectly monitoring the ﬂuorescent lifetime decay. A planar ﬁlm containing oxygen sensitive ﬂuorophores interacts with a liquid solution, where the percent DO dictates the ﬂuorescent lifetime decay. Amplitude modulated LED emission is created using an electrically implemented oscillator, exciting the oxygen sensitive ﬂuorophores. The emission light from the ﬂuorophores is detected by a photodiode and conditioned. The timing characteristics of the excitation and emission light waveforms are interpreted by a microcontroller. Time delay values have been correlated to actual percent DO values experimentally, and appropriate data modeling has been implemented for calibration purposes. This design is appropriate for application in bioreactors, presenting a functional and cost effective design. Future research can be performed to extrapolate the microcontroller platform to host a pH module, cell number module and glucose module, providing sufﬁcient feedback to an automated bioreactor systems.</p> / Master of Applied Science (MASc) optical dissolved oxygen bioreactors design frequency domain fluoroscopy cost effective Biological Engineering Biomedical Biomedical devices and instrumentation Biotechnology Electrical and Electronics Electromagnetics and photonics Systems and integrative engineering Biological Engineering
22	Power Mobility Sensor Data Collection Verified through Standardized Pediatric Assessments Rodriguez-Velez, Ayshka Elise 01 January 2018 (has links) The collaboration between the School of Engineering and the Department of Physical Therapy at the University of North Florida has introduced the possibility of creating a new environment for pediatric physical therapy assessments. There are currently no methods for remotely monitoring children with impairments. However, with embedded sensor technology in the form of power mobility and accepted therapy assessment tools, remote monitoring can become a possibility. As a part of this work, a ride-on toy car was developed as a remote monitoring device and a case study with a child with a mobility impairment was used as a proof of concept. In this thesis, the background information on the project, the case study diagnosis and history, and the model used to develop this project are detailed. Thesis University of North Florida UNF Pediatrics Physical Therapy Electrical Engineering Engineering Biomedical Engineering Assistive Technology Biomedical Biomedical Devices and Instrumentation Electrical and Electronics Physical Therapy Systems and Integrative Engineering
23	Vision Beyond Optics: Standardization, Evaluation and Innovation for Fluorescence Microscopy in Life Sciences Huisman, Maximiliaan 01 April 2019 (has links) Fluorescence microscopy is an essential tool in biomedical sciences that allows specific molecules to be visualized in the complex and crowded environment of cells. The continuous introduction of new imaging techniques makes microscopes more powerful and versatile, but there is more than meets the eye. In addition to develop- ing new methods, we can work towards getting the most out of existing data and technologies. By harnessing unused potential, this work aims to increase the richness, reliability, and power of fluorescence microscopy data in three key ways: through standardization, evaluation and innovation. A universal standard makes it easier to assess, compare and analyze imaging data – from the level of a single laboratory to the broader life sciences community. We propose a data-standard for fluorescence microscopy that can increase the confidence in experimental results, facilitate the exchange of data, and maximize compatibility with current and future data analysis techniques. Cutting-edge imaging technologies often rely on sophisticated hardware and multi-layered algorithms for reconstruction and analysis. Consequently, the trustworthiness of new methods can be difficult to assess. To evaluate the reliability and limitations of complex methods, quantitative analyses – such as the one present here for the 3D SPEED method – are paramount. The limited resolution of optical microscopes prevents direct observation of macro- molecules like DNA and RNA. We present a multi-color, achromatic, cryogenic fluorescence microscope that has the potential to produce multi-color images with sub-nanometer precision. This innovation would move fluorescence imaging beyond the limitations of optics and into the world of molecular resolution. microscopy nanoscopy fluorescence cryogenic cryo-fluorescence cryoFM FM 3DSPEED SPEED standardization PSF PSF engineering achromatic dichroic multi-color super-resolution imaging imaging standard optics adaptive optics meta-data meta MetaMax calibration characterization back-projection image reconstruction pseudo-tomography model-based reconstruction chromatic aberrations cryogenic imaging 4D-PSF calibration tool Bioimaging and Biomedical Optics Biological Engineering Biomedical Devices and Instrumentation Biophysics Computer-Aided Engineering and Design Electrical and Electronics Electromagnetics and Photonics Electro-Mechanical Systems Engineering Physics Molecular Biology Nanoscience and Nanotechnology Numerical Analysis and Computation Optics Other Engineering Systems and Integrative Engineering

Page generated in 0.1342 seconds