Abstract Food hypersensitivities are becoming increasingly common worldwide. Previous studies indicate that cell mediated immunity has a role in delayed paediatric gastrointestinal food hypersensitivities, but the exact pathogenetic mechanisms are unknown. Cytotoxic activation of T-lymphocytes is known to play an important role in the pathogenesis of celiac disease (CD). The pathogenetic mechanisms of cow's milk protein sensitive enteropathy (CMSE) are largely unknown. CMSE is a non-IgE related type of food hypersensitivity with variable gastrointestinal symptoms but no visible mucosal abnormalities on light microscopy. The diagnosis is based on an open or blinded elimination/challenge test, as the endoscopic, histological and laboratory findings are generally non-specific. This thesis aims to characterize the role of lymphocyte cytotoxicity in the pathogenesis and diagnosis of CMSE in preschool and school aged children, including comparison with CD where the pathogenetic significance of cytotoxicity is well established. The study cohort consisted of 151 children, including 57 with untreated CMSE, 18 with treated CMSE, 24 with CD, and 52 controls. Using immunohistochemistry, the mucosal expressions of cytotoxic T cell-restricted intracellular antigen type 1 (TIA-1), perforin, granzyme A and B were analysed in the duodenal bulb and descending duodenum. Intraepithelial T-lymphocytes were labelled with CD3, alpha/beta and gamma/delta T cell receptor antigens. To determine the rates of overall and epithelial apoptosis as well as proliferation, the immunohistochemical TUNEL technique, M30 and Ki-67 antibodies were used. Serum levels of granzymes, CD30 and soluble Fas were studied using ELISA method. The number of intraepithelial lymphocytes with TIA-1, perforin and granzyme A containing granules was increased in CMSE. This increase was related to antigen challenge and not a constitutional abnormality. The cytotoxic reaction in CMSE differed from that in CD by being of lesser magnitude, concerning predominantly the descending duodenum and not showing signs of cytotoxicity related epithelial destruction. The serum levels of GrA, GrB and CD30 were increased in both CMSE and CD, correlating with the number of duodenal CD3+, alpha/beta and gamma/delta+ intraepithelial lymphocytes. The results strongly support the role of cell-mediated immunity in the pathogenesis of CMSE. Mucosal cytotoxic activation seems to be manifested by the release of cytoxicity related proteins in serum. This provides a new approach to the monitoring of intestinal immune activation which could help in diagnosis and in objectively monitored treatment response.

The diploma thesis deals with an automatic code generation for PLC from Siemens. The first part focuses on currently available tools for code generation and options of C# library Siemens TIA Openness. Furthermore, a design for the project structure in the TIA portal is created. Finally, a user application for automatic code generation in TIA portal v15 is designed and created, especially suitable for larger projects that could be divided into individual stations.

This master’s thesis aims to familiarize with the types of signals used in industrial automation technology. Below are the methods for their processing and transfering to the PLC control system It is described method of processing these signals using an industrial controller of the S7 - 300 and a program for creating visualizations SIMATIC WinCC V7.0. The second part of the master’s thesis deals with the implementation of the control software of a specific technological process.

TDP-43 est une protéine multifonctionnelle possédant des rôles dans la transcription, l'épissage des pré-ARNm, la stabilité et le transport des ARNm. TDP-43 interagit avec d'autres hnRNP, incluant hnRNP A2, via son extrémité C-terminale. Plusieurs membres de la famille des hnRNP étant impliqués dans la réponse au stress cellulaire, alors nous avons émis l’hypothèse que TDP-43 pouvait y participer aussi. Nos résultats démontrent que TDP-43 et hnRNP A2 sont localisés au niveau des granules de stress, à la suite d’un stress oxydatif, d’un choc thermique, et lors de l’exposition à la thapsigargine. TDP-43 contribue à la fois à l'assemblage et au maintien des granules de stress en réponse au stress oxydatif. TDP-43 régule aussi de façon différentielle les composants clés des granules de stress, notamment TIA-1 et G3BP. L'agrégation contrôlée de TIA-1 est perturbée en l'absence de TDP-43. En outre, TDP-43 régule le niveau d`ARNm de G3BP, un facteur de granule de stress de nucléation. La mutation associée à la sclérose latérale amyotrophique, TDP-43R361S, compromet la formation de granules de stress. Ainsi, la fonction cellulaire de TDP-43 s'étend au-delà de l’épissage; TDP-43 est aussi un composant de la réponse cellulaire au stress central et un acteur actif dans le stockage des ARNs. / TDP-43 is a multifunctional protein with roles in transcription, pre-mRNA splicing, mRNA stability and transport. TDP-43 interacts with other hnRNPs, including hnRNP A2, via its C-terminus and several hnRNP family members are involved in the cellular stress response. This relationship led us to investigate the role of TDP-43 in cellular stress. Our results demonstrate that TDP-43 and hnRNP A2 are localized to stress granules, following oxidative stress, heat shock, and exposure to thapsigargin. TDP-43 contributes to both the assembly and maintenance of stress granules in response to oxidative stress and differentially regulates key stress granules components including TIA-1 and G3BP. The controlled aggregation of TIA-1 is disrupted in the absence of TDP-43. In addition, TDP-43 regulates G3BP mRNA levels, a stress granule nucleating factor. A mutation associated with amyotrophic lateral sclerosis, TDP-43R361S, compromises stress granule formation. Thus, the cellular function of TDP-43 extends beyond splicing and places TDP-43 as a participant of the central cellular response to stress and an active player in RNA storage.

NO ROMANCE DE MARCEL PROUST Em busca do tempo perdidodesfilam ante os olhos do leitorum compêndio de seres enigmáticos, fragmentários, que oferecem em si, ao longo da obra, uma gama de novas imagens e possibilidades. Desse complexo conjunto de seres imprevisíveis, há um caso particular de uma personagem secundária. Figuraaparentemente dentro da narrativa como um ser anedótico, apenas para compor um ambiente cômico, pueril dentro de Combray, a cidadezinha prosaica de infância do herói do romance: a tia Léonie. O objetivo do trabalho que apresentamos não é somente analisar esse ser aparentemente risório, a eterna doente de Combray. Sobretudo, pretende-se mostrar dentro dopróprio romance proustiano que Léonie, como os tantos personagens desse trajeto rumo à descoberta da verdadeira vocação do herói, também colocará sua pequena pedra na composição monumental da obra por vir, pois, ao seu modo, também contribuirá para a composição daquele que renunciará à vida, se reservando apenas o essencial, para compor seu romance. / IN THE NOVEL OF MARCEL PROUST\'S \"In Search of Lost Time\", parade before the reader\'s eyes, a compendium of enigmatic, fragmentary beings, offering itself, throughout the work, a range of new images and possibilities. In this complex set of unpredictable beings, there is a particular case of a secondary character, apparently figuring in the narrative as being anecdotal, just to make a comic, childlike environment in Combray, the prosaic town of the novel heros childhood: Aunt Léonie. The objective of the present work is not only to analyze this seemingly be risorius, \"the eternal sick of Combray.\" Above all, it presents in the Proustian novel itself that Léonie, as so many characters that path towards the discovery of the true vocation of the hero, also put your little stone in monumental composition of the work to come, for, in his own way, also contribute for the composition that he would resign to life, reserve to itself only the essentials, in order to compose his novel.

This master's thesis deals with the design of a differential high-gain transimpedance amplifier in TSMC's 0.18 um mixed signal process that utilizes a DC photodiode current cancellation loop and a switching automatic gain control (AGC) with a bilinear gain curve. The amplifier is designed to satisfy the demands of Optical Coherence Tomography applications where the receiver is expected to measure the envelope power of an amplitude modulated sinusoidal optical signal that incorporates a large DC component. Methods of increasing dynamic range and gain linearity through the use of DC photodiode current cancellation and bilinear gain are explored. Effects of changing DC photodiode current on the overall system response is also demonstrated.

TDP-43 est une protéine multifonctionnelle possédant des rôles dans la transcription, l'épissage des pré-ARNm, la stabilité et le transport des ARNm. TDP-43 interagit avec d'autres hnRNP, incluant hnRNP A2, via son extrémité C-terminale. Plusieurs membres de la famille des hnRNP étant impliqués dans la réponse au stress cellulaire, alors nous avons émis l’hypothèse que TDP-43 pouvait y participer aussi. Nos résultats démontrent que TDP-43 et hnRNP A2 sont localisés au niveau des granules de stress, à la suite d’un stress oxydatif, d’un choc thermique, et lors de l’exposition à la thapsigargine. TDP-43 contribue à la fois à l'assemblage et au maintien des granules de stress en réponse au stress oxydatif. TDP-43 régule aussi de façon différentielle les composants clés des granules de stress, notamment TIA-1 et G3BP. L'agrégation contrôlée de TIA-1 est perturbée en l'absence de TDP-43. En outre, TDP-43 régule le niveau d`ARNm de G3BP, un facteur de granule de stress de nucléation. La mutation associée à la sclérose latérale amyotrophique, TDP-43R361S, compromet la formation de granules de stress. Ainsi, la fonction cellulaire de TDP-43 s'étend au-delà de l’épissage; TDP-43 est aussi un composant de la réponse cellulaire au stress central et un acteur actif dans le stockage des ARNs. / TDP-43 is a multifunctional protein with roles in transcription, pre-mRNA splicing, mRNA stability and transport. TDP-43 interacts with other hnRNPs, including hnRNP A2, via its C-terminus and several hnRNP family members are involved in the cellular stress response. This relationship led us to investigate the role of TDP-43 in cellular stress. Our results demonstrate that TDP-43 and hnRNP A2 are localized to stress granules, following oxidative stress, heat shock, and exposure to thapsigargin. TDP-43 contributes to both the assembly and maintenance of stress granules in response to oxidative stress and differentially regulates key stress granules components including TIA-1 and G3BP. The controlled aggregation of TIA-1 is disrupted in the absence of TDP-43. In addition, TDP-43 regulates G3BP mRNA levels, a stress granule nucleating factor. A mutation associated with amyotrophic lateral sclerosis, TDP-43R361S, compromises stress granule formation. Thus, the cellular function of TDP-43 extends beyond splicing and places TDP-43 as a participant of the central cellular response to stress and an active player in RNA storage.

In the last decades our knowledge concerning cardiovascular risk factors has grown rapidly through results from longitudinal studies. However, despite new treatment, in Western countries coronary heart disease remains the leading cause of death and stroke is still the leading cause of severe disability. The studies reported in these papers examine the relationships between stroke/transient ischaemic attack (TIA) and a number of different factors measured on two different occasions in men born in Uppsala 1920-1924 and are epidemiological in their character. The findings indicate that in addition to already established risk factors, indices of an unhealthy dietary fat intake and high serum lipoprotein(a) are independent predictors of stroke/TIA. Among different glucometabolic variables a low insulin sensitivity index derived from the euglycaemic insulin clamp and proinsulin carries a high predictive value for later stroke, independently of diabetes. Moreover, cognitive test performance measured with Trail Making Test B at age 70 is a strong and independent predictor of brain infarction, indicating that the risk is already increased in the subclinical phase of milder cognitive dysfunction. Performance at a pre-stroke Trail Making Test is also of predictive value for mortality after first-ever stroke/TIA, but none of the studied pre-stroke variables or cognitive tests was found to be related to dependency after an event. In summary these studies provide further knowledge about predictors of stroke and of mortality after first-ever stroke. They also indicate the possible importance of new markers of risk, such as the level of lipoprotein(a), profile of fatty acids in the diet, low insulin sensitivity derived from clamp investigations, level of proinsulin, and cognitive performance measured with Trail Making Tests.

NO ROMANCE DE MARCEL PROUST Em busca do tempo perdidodesfilam ante os olhos do leitorum compêndio de seres enigmáticos, fragmentários, que oferecem em si, ao longo da obra, uma gama de novas imagens e possibilidades. Desse complexo conjunto de seres imprevisíveis, há um caso particular de uma personagem secundária. Figuraaparentemente dentro da narrativa como um ser anedótico, apenas para compor um ambiente cômico, pueril dentro de Combray, a cidadezinha prosaica de infância do herói do romance: a tia Léonie. O objetivo do trabalho que apresentamos não é somente analisar esse ser aparentemente risório, a eterna doente de Combray. Sobretudo, pretende-se mostrar dentro dopróprio romance proustiano que Léonie, como os tantos personagens desse trajeto rumo à descoberta da verdadeira vocação do herói, também colocará sua pequena pedra na composição monumental da obra por vir, pois, ao seu modo, também contribuirá para a composição daquele que renunciará à vida, se reservando apenas o essencial, para compor seu romance. / IN THE NOVEL OF MARCEL PROUST\'S \"In Search of Lost Time\", parade before the reader\'s eyes, a compendium of enigmatic, fragmentary beings, offering itself, throughout the work, a range of new images and possibilities. In this complex set of unpredictable beings, there is a particular case of a secondary character, apparently figuring in the narrative as being anecdotal, just to make a comic, childlike environment in Combray, the prosaic town of the novel heros childhood: Aunt Léonie. The objective of the present work is not only to analyze this seemingly be risorius, \"the eternal sick of Combray.\" Above all, it presents in the Proustian novel itself that Léonie, as so many characters that path towards the discovery of the true vocation of the hero, also put your little stone in monumental composition of the work to come, for, in his own way, also contribute for the composition that he would resign to life, reserve to itself only the essentials, in order to compose his novel.

The thesis "Load testing of non-IT parts data centers" deals with the theoretical analysis of data centers with a focus on non-IT part and theoretical preparation for measuring stress tests focused mainly on cooling. The theory is followed by a practical part of the PEC measurement by CATEGORY a.s .. This sample measurement carried out at the company premises clearly outlines the importance of performing similar tests. The measurement is thoroughly evaluated and suggestions are made to improve the operation. The next part of the practical part contains simulation of sample measurement. This simulation serves as a convenient addition and comparison. Also this simulation, the importance of similar stress tests can be justified.