Thesis (MPH.) -- University of Limpopo, 2020 / Background: In South Africa, the Choice on Termination of Pregnancy Act (CTOP) (No. 92 of 1996) promotes a woman's reproductive right and choice to have an early, safe and legal abortion. Pregnancy termination among young women constitutes a public health problem particularly in South Africa where high prevalence of abortion has been recently recorded. HIV acquisition is increased two to four-fold during pregnancy, due to biological and behavioural factors including immunological changes, hormonal changes affecting the genital tract mucosa, higher frequency of unprotected sex and incident sexually transmitted infections (STIs) during pregnancy. There is a growing interest in exploring maternal mental health effects of unintended pregnancies. However, the evidence base from a small number of available studies is characterized by considerable variability, inconsistency and inconclusive findings. Therefore, the primary objective of this study was to investigate the prevalence of HIV and its association with termination of pregnancy at Seshego Zone 4 clinic in Limpopo Province. Methodology: A cross-section descriptive retrospective review study in which convenience sampling of the records of women who terminated pregnancies was used in this study. The key variable of interest in this study was HIV results and all patients records without evidence of HIV testing, and the associated results were excluded. A self-designed data extraction tool was used to extract the data from patients records and tool covered variables such as the age of the women, educational status, marital status, occupational status, year and month of termination of pregnancy, gestational age, parity, and gravidity, method of contraceptive used, HIV status, ARV and ARV regimens. Data analysis was done using the STATA statistical software version 12 for Windows (STATA Corporation, College Station, Texas). vi Results: The mean age was 24.98 years SD±14.4 and majority of women who terminated pregnancies were in the age group 20 – 24 years at 35.7% and the least number of women who terminated pregnancies were in the age groups ≥ 40 years and ≤ 14 years at 2.3% and 0.3% respectively. Majority of the women who terminated pregnancies had parity of 1 – 2 at 47.4% followed by parity of zero at 42.3% and 3 – 4 at 9.9%. Majority of the women who terminated pregnancies were in gravida 1 at 42.8% followed by those with gravida 2 at 27.1% and those who were pregnant between the 3rd and 4th time were 26.9%. There was a statistical significance difference (p<0.001) of the use of contraceptives by age groups and also in relation to parity and similarly to gravidity. The prevalence of HIV amongst women who terminated pregnancies in the current study was found to be 11.6% and this was high in 2018 at 10.5% followed by 2019, 2015 and 2016 at 10.3%, 9.2% and 9.1% respectively. The prevalence of HIV amongst women who terminated pregnancies increased with increasing level of education from 4.1% amongst women who had primary or no educational level the followed by 9,0% and 13.6% in women who had secondary and tertiary educational level respectively. The risk of women who terminate pregnancies being HIV positive in the current study increased significantly with increasing age as older women were 1.9 times more likely to be HIV positive as compared to younger ones (p=0.004) Conclusion: The findings of this study highlight the need to address the structural socio-economic drivers of the HIV epidemic among women of child-bearing age. Women of child-bearing age in this setting have large unmet reproductive health needs. Structural interventions, such as increasing contraceptive use which may be useful for reducing the burden of unplanned pregnancies. Key concepts Human immunodeficiency virus, Acquired immunodeficiency syndrome, Termination of pregnancy, Parity and Gravidity.

Original published work submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctorate of Philosophy in Virology. Johannesburg 2017. / Introduction: HIV-infected and HIV-exposed-uninfected children have a heightened susceptibility to some vaccine preventable disease. There is a paucity of data on immunogenicity of vaccines in these children, including HIV-infected children who are initiated on early antiretroviral therapy (ART). We evaluated the effect of maternal HIV-exposure and timing of ART in HIV-infected children on antibody responses to combined diphtheria-toxoid (DT) -tetanus-toxoid (TT)-whole cell pertussis (wP) and Haemophilus influenzae type b conjugate vaccine (HibCV); monovalent hepatitis B vaccine (HepB) and live-attenuated measles vaccine (MV). Methods: Samples obtained from children aged 6–12 weeks who had been enrolled into the CIPRA-SA study were analysed. Briefly, HIV-uninfected children born to HIV-uninfected (HIV-unexposed) and HIV-infected mothers (HEU). Additionally, we enrolled perinatally HIV-infected children with CD4+%≥25% randomized to deferred-ART (i.e. initiated when clinically or immunologically indicated per the then WHO recommended treatment criteria; ART-Def) or immediate-ART initiation (i.e. initiated on ART immediately upon confirmation of HIV-infection status at 4-10 weeks of age; ART-Immed). Children enrolled in the ART-Immed arm were further randomized to interrupt ART at one-year (ART/12m) or two-years of age (ART/24m). Additionally, a convenience sample of HIV-infected children with CD4+<25% initiated on immediate-ART was enrolled (ART-CD4+<25%). Children received a primary series of DTwP-HibCV/HepB at 6, 10 and 14 weeks of age; and MV at 40 weeks of age. Booster dose of DTwP and MV was given at 15-18 months of age. Sampling time-points were: prior to the first dose of vaccine, four weeks after the third dose (18 weeks age), 24 weeks after the third dose (39.3 weeks of age), at the time of the booster dose (15- 18 months age), two to four weeks after the booster dose and at 24 months of age. Samples were analysed for antibodies for DT, TT, PT, FHA, HepB measured by Luminex microbead-immunoassay; and MV antibodies were quantified by an indirect enzyme immunoassay. Results: Antibody kinetics and response to primary series of DTwP-HibCV/HepB: Pre-vaccination GMCs were higher in HIV-unexposed than HEU children for TT, but lower for HepB, DT and FHA. Post-vaccination, sero-conversion, sero-protection and GMCs were similar in HEU and HIV-unexposed children for all vaccines. Furthermore, GMCs were higher in HIV-unexposed for TT, DT, HepB and FHA than in ART-Immed children; and for TT, HepB and PT than in ART-Def children. Nevertheless, there was no difference in proportion of HIV-unexposed and HIV-infected children who developed sero-protective vaccine-specific antibody levels post-vaccination. The timing of ART initiation generally did not affect immune responses to vaccines between HIV-infected groups. Antibody kinetics and booster responses to DTwP-HibCV/HepB vaccines: Pre-booster GMCs were generally higher in HIV-unexposed than HIV-infected children for all vaccine epitopes. Post-booster and at 24 months of age the ART-Def group had lower GMCs (except to FHA), and were less likely to have sero-protective antibody levels compared to HIV-unexposed group. Also, post-booster and at 24 months of age, GMC were generally higher in HIV-unexposed than ART-Immed children, and a higher percentage of HIV-unexposed than ART-Immed children maintained antibody levels ≥1IU/ml to TT and DT at 24 months of age. The GMCs and percentage of children with sero-protective thresholds were similar pre-booster and at 24 months of age between HIV-unexposed and HEU children. Antibody kinetics and response to measles virus vaccine: At 7.3 weeks of age, the proportion with sero-protective titers was higher in HIV-unexposed (65.2%) compared to any HIV-infected group (range: 16.7% to 41.8%); but dropped to <17% in all Groups at age 19.6 weeks. Twenty-eight weeks following the first measles-vaccine, ART/12m were less likely to have sero-protective titers (79.3%) compared to HIV-unexposed (94.8%; p<0.001), ART-Def (95.7%; p=0.003) or ART/24m (92.1%; p=0.02). Although the proportion with sero-protective levels were similar between groups immediately post-booster dose, this was lower in HEU (79.6%; p=0.002) and ART/12m (80.3%; p=0.01) compared to HIV-unexposed (94.3%) 41-weeks later. Conclusion: Primary vaccination with DTwP-HibCV/HBV of HIV-infected children initiated on early-ART confers similar immunity compared to HIV-unexposed children. HIV-infected children had poor anamnestic responses, if ART was not initiated prior to primary vaccination. In contrast, the memory response and persistence of antibody to most vaccine epitopes were similar between HIV-unexposed and HEU children. Increased waning of vaccine induced immunity over a 24 month period in ART-Def, ART/12m and HEU children following MV booster-dose; indicating the need for further booster doses after two-years of age in these children. I recommend close monitoring of HEU children, as this group makes up most children born to HIV-infected mothers and what facets of the immune system have been impacted by maternal exposure to HIV. / MT2017

Patients infected with the human immunodeficiency virus (HIV+) are living longer and at heightened risk for developing cardiovascular events (CVEs). Commonly used prediction tools appear to misrepresent their CVE risk to varying degrees and in varying directions. Inclusion of markers of cellular infection, chronic immune activation and/or systemic inflammation into risk models might provide better predictive accuracy. Observational studies assessing the relationship of high-sensitivity C-reactive protein (hs-CRP) to CVE in HIV+ patients have reported inconsistent findings. This review of published studies attempted to determine if the available evidence supports its potential use in new models for stable, treated HIV+ patients. We searched the PubMed database using keywords and combinations of "HIV" AND "cardiovascular risk" AND "CRP". Papers presenting original analyses, associating hs-CRP concentration as an independent variable to hard cardiovascular outcomes (myocardial infarction and cardiovascular death), or to hard CVE as part of a composite endpoint, were included. Five observational studies met inclusion/exclusion criteria for review. Three papers identified an association between elevated hs-CRP and CVE, while two others failed to find any significant association. All reports were heterogeneous in terms of independent variables, controls, and designs. The larger and more rigorous studies, employing higher rates of confounder controls and more objective endpoints in their composites, showed positive associations. Though not conclusive, the preponderance of the evidence at this time supports CRP as a potentially valuable factor to be studied in prospective cardiovascular risk prediction investigations in HIV+ patients.

This descriptive research used the Health Belief Model (HBM) to assess HIV/AIDS knowledge, perceived severity, perceived susceptibility, and behavioral practices within the population throughout the State of Sonsonate, El Salvador. Geographic Information Systems (GIS) was also utilized to display the results of this population study. The study population was composed of 15-39 year olds consisting of a total sample size of 1,500 (250 participants from each of the six cities). The basis of this study was to evaluate where this population fit within the Health Belief Model (HBM) and also to practically represent the results pictorially. Also, this information was collected so that the government of El Salvador could see and understand where their educational deficits existed so that future programs could be established to alleviate these discrepancies. An instrument consisting of 65 items including demographics, knowledge, perceived severity, perceived susceptibility, and behavioral practices was used for the general population. The analysis of the data was two-fold using Geographic Information Systems (GIS) mapping and statistical analysis. GIS mapping was used to graphically pinpoint areas of knowledge deficit and misinformation about HIV/AIDS. Results were based on a general population of 1,454. Various indices were created to show the level of knowledge or frequency of safe behavioral practices. The Perceived Severity and Perceived Susceptibility sections were not aggregated into an index but rather treated as individual variables. An item analysis of the questionnaire found that on average the general population responded correctly to 78% of the knowledge questions. However, a total of 11 questions in the knowledge section had less 75% (n=385) of the general population answering correctly. Another 3 questions in the behavior section were also found to have less than 75% (n=385) indicating safe behavioral practices. Linear regression analyses were performed to explore correlations between the areas of demographics, knowledge level, perceived severity, and perceived susceptibility to safe behavior. GIS maps were created to pictorially show where this population's deficiencies were in regard to the HBM. This research helped to pinpoint where this population fits within the construct of the Health Belief Model so that future educational efforts can be targeted in geographic areas with the greatest need. This will help to move this sample toward behavioral change. / Ph. D.

人類免疫缺陷病毒 (HIV) 是人獲得性免疫缺陷綜合征 (愛滋病，AIDS) 的致病原，2010年全球有180萬人死於愛滋病，HIV/AIDS已成為全球健康的嚴重挑戰。人類免疫缺陷病毒與乙型肝炎病毒 (HBV) ，丙型肝炎病毒 (HCV) 的合併感染非常普遍，已演變成具有嚴重臨床後果的新健康問題。儘管對於人類免疫缺陷病毒的研究已有很大的進展，但由於受研究模型的限制，人體免疫系統對人類免疫缺陷病毒感染的應答，特別是對乙型肝炎病毒，丙型肝炎病毒與人類免疫缺陷病毒合併感染的免疫應答，仍值得進一步的闡明。 / 在本研究中，我們首先對深圳人類免疫缺陷病毒，乙型肝炎病毒，丙型肝炎病毒合併感染的流行情況進行研究。共選取914份人類免疫缺陷病毒感染者的血漿，經過對乙型肝炎病毒表面抗原 (HBsAg) 和抗丙型肝炎病毒抗體 (anti-HCV) 的檢測，發現10.9% (100/914) 的被檢測者是人類免疫缺陷病毒/乙型肝炎病毒合併感染，14.6% (133/914) 為人類免疫缺陷病毒/丙型肝炎病毒合併感染，3.7% (34/914) 為人類免疫缺陷病毒/乙型肝炎病毒/丙型肝炎病毒三重感染。多元邏輯回歸分析證明人類免疫缺陷病毒傳染的危險行為與合併感染顯著相關聯。大多數的人類免疫缺陷病毒/乙型肝炎病毒合併感染者都是通過性接觸感染人類免疫缺陷病毒，包括異性傳播與同性傳播 (95/100, 95%); 大多數的人類免疫缺陷病毒/丙型肝炎病毒合併感染者是靜脈注射吸毒者 (89/133, 66.9%); 人類免疫缺陷病毒/乙型肝炎病毒/丙型肝炎病毒三重感染者中，大多數是靜脈注射吸毒者 (28/34, 82.4%)。靜脈注射吸毒人群中，大部分是男性 (108/122, 88.5%)，約半數人的年齡介乎27至32歲 (56/122, 45.9%) 。有接近一半的經過血液和血液製品傳播人類免疫缺陷病毒的人是人類免疫缺陷病毒/丙型肝炎病毒合併感染者 (10/23, 43.5%) 。性別與人類免疫缺陷病毒感染的危險行為有顯著關係，大部份的靜脈注射吸毒者是男性。 / 進一步，我們利用酶聯免疫吸附測定法 (ELISA) 檢測深圳愛滋病陽性樣本血漿中白細胞介素27 (IL-27) 的濃度。結果顯示，對比健康參照者，人類免疫缺陷病毒單獨感染者，人類免疫缺陷病毒/乙型肝炎病毒合併感染者，人類免疫缺陷病毒/丙型肝炎病毒合併感染者的血漿IL-27濃度顯著升高。隨後我們進一步發現，人類免疫缺陷病毒單獨感染組，人類免疫缺陷病毒/乙型肝炎病毒，人類免疫缺陷病毒/乙型肝炎病毒/丙型肝炎病毒合併感染組之間的血漿IL-27濃度沒有顯著差異，而人類免疫缺陷病毒/丙型肝炎病毒合併感染組與人類免疫缺陷病毒/乙型肝炎病毒/丙型肝炎病毒三重感染組的血漿IL-27濃度差異顯著。我們還發現人類免疫缺陷病毒單獨感染組中，血漿IL-27濃度與CD4⁺ T 淋巴細胞數量顯著正相關 (r = 0.177, P = 0.034)。 / 我們進一步分析了人類免疫缺陷病毒和丙型肝炎病毒的病毒載量對血漿IL-27濃度的影響，發現HIV單獨感染組中人類免疫缺陷病毒載量與血漿IL-27濃度沒有顯著相關 (r = - 0.063, P = 0.679)，而人類免疫缺陷病毒/丙型肝炎病毒合併感染組中，人類免疫缺陷病毒載量與血漿IL-27濃度顯著正相關 (r = 0.362, P = 0.049)。人類免疫缺陷病毒/丙型肝炎病毒合併感染組中，人類免疫缺陷病毒載量與丙型肝炎病毒載量缺少顯著線性關聯 (r = - 0.072, P = 0.704)，而人類免疫缺陷病毒/丙型肝炎病毒合併感染組可根據人類免疫缺陷病毒與丙型肝炎病毒的病毒載量再細分成血漿IL-27濃度差異顯著的三組 (P = 0.014) ， 丙型肝炎病毒載量與血漿IL-27濃度缺少顯著關聯 (r = - 0.119, P = 0.530) 。 / 我們利用TaqMan®等位基因分型技術測定深圳男同性戀人群中IL-27 p28基因的單核苷酸多態性 (SNP)。結果顯示，人類免疫缺陷病毒感染組IL-27 p28 -964A/G 和4603G/A的基因型與健康男同性戀參照組的基因型沒有顯著差異， IL-27 p28 -964A/G 和4603G/A的等位基因比率也沒有顯著差異。結果也顯示，IL-27 p28 2905T/G的TG基因型可減少2.77倍的人類免疫缺陷病毒感染風險，等位基因G可減少2.72倍的人類免疫缺陷病毒感染風險。連鎖不平衡在IL-27 p28 -964A/G 和2905T/G 中存在 ( / 綜上所述， 在本研究中，我們首次調查了深圳人類免疫缺陷病毒，乙型肝炎病毒，丙型肝炎病毒合併感染的流行情況，並分析了合併感染的風險因素。 發現人類免疫缺陷病毒單獨感染者，人類免疫缺陷病毒/乙型肝炎病毒合併感染者， 及人類免疫缺陷病毒/丙型肝炎病毒合併感染者的血漿IL-27濃度比健康參照組顯著地升高；人類免疫缺陷病毒單獨感染組中，血漿IL-27濃度與CD4⁺ T淋巴細胞數量顯著正相關。人類免疫缺陷病毒/丙型肝炎病毒合併感染組中，人類免疫缺陷病毒載量與血漿IL-27濃度顯著正相關 (r = 0.362, P = 0.049)。分析深圳男同性戀人群IL-27 p28基因的單核苷酸多態性，發現IL-27 p28 2905T/G 與人類免疫缺陷病毒感染相關，GGG單型可降低男同性戀人群人類免疫缺陷病毒感染的風險。 / Human Immunodeficiency Virus (HIV) is the causative agent of Acquired Immunodeficiency Syndrome (AIDS); HIV/AIDS caused 1.8 million deaths world-widely in 2010 and became a major global health challenge. HIV co-infections with Hepatitis B virus (HBV), Hepatitis C virus (HCV) are common and have emerged into new health problems with severe clinical consequences. Since the discovery of HIV, massive progress in understanding of the pathogen has been achieved. Due to the restriction of research model, how human immune system responds to HIV infection, particularly, to HBV or HCV co-infections is still worthy further elucidation. / A cohort study was first conducted in Shenzhen regarding the seroprevalence of HBV, HCV infections among HIV-infected population. Totally 914 HIV positive individuals were recruited in the study and tested for HBsAg and anti-HCV antibodies. The results showed a 10.9% (100/914) HIV/HBV co-infection rate, 14.6% (133/914) HIV/HCV co-infection prevalence and 3.7% (34/914) HIV/HBV/HCV triple-infection prevalence. Multivariate logistic regression revealed that HIV transmission risk behavior was significantly associated with HIV, HBV, HCV co-infections. Most HIV/HBV co-infection cases got HIV through sexual contact including heterosexual and homosexual behaviors (95/100, 95%); while most HIV/HCV co-infection subjects were injection drug users (IDUs) (89/133, 66.9%). In the case of HIV/HBV/HCV triple-infection, IDUs accounted for a large ratio (28/34, 82.4%). Among IDUs, most of them were male (108/122, 88.5%) and nearly half were aged 27 to 32 years old (56/122, 45.9%). Near half people who got HIV through blood and blood products were HIV/HCV co-infected (10/23, 43.5%). Gender has a significant correlation with HIV risk behavior and most IDUs were male. / Next, we applied ELISA to test HIV positive clinical samples and proved that plasma interleukin-27 (IL-27) level was significantly elevated in HIV mono-infected, HIV/HBV co-infected and HIV/HCV co-infected subjects when compared with healthy controls. Later, we further revealed that plasma IL-27 titer was not significantly varied among HIV, HBV and HCV co-infections except between HIV/HCV co-infections and HIV/HBV/HCV triple-infections. We also observed a significant positive correlation between CD4⁺ T cell counts and plasma IL-27 titer within HIV mono-infected group (r = 0.177, P = 0.034). / We further analyzed the impact HIV and HCV viral loads on plasma IL-27 titer. We found there was no significant correlation between HIV viral load and IL-27 titer among HIV mono-infected individuals (r = - 0.063, P = 0.679); while a significant positive correlation was observed between HIV viral load and IL-27 titer in HIV/HCV co-infected individuals (r = 0.362, P = 0.049). In the case of HIV/HCV co-infection, there was no significant linear correlation between HIV and HCV viral loads (r = - 0.072, P = 0.704) but exist obvious subdivision of samples in terms of HIV and HCV viral loads with significant IL-27 titer variance (P = 0.014). No correlation was observed between HCV viral load and IL-27 titer (r = - 0.119, P = 0.530). / IL-27 p28 polymorphisms were genotyped with TaqMan® Allelic Discrimination Assay in Chinese men who have sex with men (MSM) population in Shenzhen and the results revealed that proportions of IL-27 p28 -964A/G and 4603G/A genotypes were not significantly different from the healthy controls; IL-27 p28 -964A/G and 4603G/A allele frequencies were similar between HIV positive MSM group and healthy control MSM group. Results also showed that for IL-27 p28 2905T/G polymorphism, TG genotype has a 2.77-fold decreased risk of HIV susceptibility and subjects with G allele has a 2.72-fold decreased risk of HIV susceptibility. Linkage disequilibrium (LD) coefficients were observed between IL-27 p28 -964A/G and 2905T/G ( / In conclusion, the seroprevalences of HBV and HCV infection among HIV positive population in Shenzhen were surveyed and risk factors associated with co-infections were analyzed. Plasma IL-27 titer was significantly elevated in HIV mono-infected, HIV/HBV co-infected and HIV/HCV co-infected individuals. IL-27 level was correlated with CD4⁺ T cell counts within HIV mono-infected people. A significant positive correlation was found between HIV viral load and IL-27 titer in HIV/HCV co-infected individuals (r = 0.362, P = 0.049). IL-27 p28 2905T/G was associated with individual susceptibility to HIV infection and haplotype GGG showed a protective role in restricting HIV infection in MSM population. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / He, Lai. / "October 2012." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 135-155). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese. / Abstract (English) --- p.iii / Abstract (Chinese) --- p.vi / Acknowledgements --- p.ix / Contents --- p.x / List of Tables --- p.xv / List of Figures --- p.xvi / Abbreviations --- p.xvii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Human Immunodeficiency Virus --- p.1 / Chapter 1.1.1 --- HIV virology --- p.1 / Chapter 1.1.1.1 --- HIV structure and genome organization --- p.1 / Chapter 1.1.1.2 --- HIV life cycle --- p.3 / Chapter 1.1.1.3 --- HIV genotypes --- p.5 / Chapter 1.1.2 --- HIV epidemiology --- p.6 / Chapter 1.1.2.1 --- Global HIV epidemiology --- p.6 / Chapter 1.1.2.2 --- HIV epidemiology in China --- p.9 / Chapter 1.1.3 --- HIV pathogenesis --- p.13 / Chapter 1.1.3.1 --- Natural history of HIV infection --- p.13 / Chapter 1.1.3.2 --- HIV transmission --- p.15 / Chapter 1.1.3.3 --- HIV tropism --- p.17 / Chapter 1.1.4 --- Immune responses to HIV infection --- p.19 / Chapter 1.1.4.1 --- Innate immune response --- p.19 / Chapter 1.1.4.2 --- Adaptive immune response --- p.21 / Chapter 1.1.5 --- Diagnosis --- p.24 / Chapter 1.1.6 --- HIV prevention --- p.25 / Chapter 1.1.7 --- Anti-HIV therapy --- p.25 / Chapter 1.1.8 --- Hepatitis B virus, Hepatitis C virus infection --- p.26 / Chapter 1.1.8.1 --- HBV infection natural history, diagnosis, disease progression and epidemiology --- p.26 / Chapter 1.1.8.2 --- HCV infection natural history, diagnosis, disease progression and epidemiology --- p.30 / Chapter 1.1.9 --- HIV, HBV, HCV co-infections --- p.32 / Chapter 1.2 --- Interleukin-27 --- p.36 / Chapter 1.2.1 --- Biology of IL-27 --- p.36 / Chapter 1.2.2 --- IL-27 on immune system --- p.37 / Chapter 1.2.3 --- IL-27 anti-tumor properties --- p.38 / Chapter 1.2.4 --- IL-27 antiviral features --- p.40 / Chapter 1.2.5 --- IL-27 with hepatitis --- p.41 / Chapter 1.3 --- Single-nucleotide polymorphisms (SNPs) --- p.42 / Chapter 1.3.1 --- Types of SNPs --- p.43 / Chapter 1.3.2 --- Functions of SNPs --- p.43 / Chapter 1.4 --- Objectives of the study --- p.45 / Chapter Chapter 2 --- Seroprevalence of HBV, HCV infection among HIV positive individuals in Shenzhen --- p.52 / Chapter 2.1 --- Introduction --- p.52 / Chapter 2.2 --- Materials and methods --- p.54 / Chapter 2.2.1 --- Study participants --- p.54 / Chapter 2.2.2 --- Measure of HBV, HCV seroprevalence --- p.55 / Chapter 2.2.3 --- Statistical analysis --- p.60 / Chapter 2.3 --- Results --- p.61 / Chapter 2.3.1 --- HIV infection in Shenzhen --- p.61 / Chapter 2.3.2 --- Seroprevalence of HBV, HCV infection among HIV positive individuals in Shenzhen --- p.61 / Chapter 2.4 --- Discussion --- p.65 / Chapter 2.4.1 --- HIV infection in Shenzhen --- p.65 / Chapter 2.4.2 --- HIV, HBV, HCV co-infections in Shenzhen --- p.68 / Chapter 2.4.3 --- Limitations of the study --- p.71 / Chapter Chapter 3 --- Upregulation of Interleukin-27 titer in HIV infected persons --- p.78 / Chapter 3.1 --- Introduction --- p.78 / Chapter 3.2 --- Materials and methods --- p.80 / Chapter 3.2.1 --- Study participants --- p.80 / Chapter 3.2.2 --- Measure of HIV, HBV, HCV infection --- p.80 / Chapter 3.2.3 --- Detection of IL-27 in plasma --- p.81 / Chapter 3.2.4 --- CD4 counting --- p.84 / Chapter 3.2.5 --- Statistical analysis --- p.84 / Chapter 3.3 --- Results --- p.84 / Chapter 3.3.1 --- Demographics of study participants --- p.84 / Chapter 3.3.2 --- Upregulation of IL-27 levels in HIV infected persons --- p.85 / Chapter 3.3.3 --- Correlation of plasma IL-27 titer with CD4⁺ T cell count --- p.86 / Chapter 3.4 --- Discussion --- p.86 / Chapter Chapter 4 --- Impact of HIV, HCV viral loads on Interleukin-27 titer among Antiretroviral Therapy- Naïve HIV positive Chinese --- p.95 / Chapter 4.1 --- Introduction --- p.95 / Chapter 4.2 --- Materials and methods --- p.96 / Chapter 4.2.1 --- Study participants --- p.97 / Chapter 4.2.2 --- HIV, HBV and HCV Serological assays --- p.97 / Chapter 4.2.3 --- CD4 counting --- p.97 / Chapter 4.2.4 --- Detection of plasma IL-27 --- p.98 / Chapter 4.2.5 --- Quantification of HIV, HCV viral loads --- p.98 / Chapter 4.2.6 --- Statistical analysis --- p.102 / Chapter 4.3 --- Results --- p.102 / Chapter 4.3.1 --- Demographics of study participants --- p.102 / Chapter 4.3.2 --- Plasma IL-27 was elevated in HIV-positive persons --- p.103 / Chapter 4.3.3 --- Correlation of IL-27 titer and CD4⁺ T cell count --- p.103 / Chapter 4.3.4 --- Correlation of HIV viral load and IL-27 titer --- p.104 / Chapter 4.3.5 --- Correlation of HCV viral load and IL-27 titer --- p.104 / Chapter 4.4 --- Discussion --- p.105 / Chapter Chapter 5 --- Association of Interleukin-27 polymorphisms with the susceptibility to HIV infection in a Chinese men who have sex with men population --- p.116 / Chapter 5.1 --- Introduction --- p.116 / Chapter 5.2 --- Materials and methods --- p.118 / Chapter 5.2.1 --- Study participants --- p.118 / Chapter 5.2.2 --- HIV screening --- p.118 / Chapter 5.2.3 --- Genomic DNA extraction --- p.119 / Chapter 5.2.4 --- IL-27 p28 -964A/G, 2905T/G and 4603G/A genotyping --- p.120 / Chapter 5.2.5 --- Statistical analysis --- p.121 / Chapter 5.3 --- Results --- p.122 / Chapter 5.3.1 --- Demographics of study participants --- p.122 / Chapter 5.3.2 --- IL-27 genotypes and allele frequencies in HIV MSM and healthy MSM controls --- p.122 / Chapter 5.3.3 --- LD analysis and haplotype analysis --- p.123 / Chapter 5.4 --- Discussion --- p.124 / Chapter Chapter 6 --- Summary and perspectives --- p.130 / Chapter 6.1 --- Summary --- p.130 / Chapter 6.2 --- Perspectives --- p.132 / Bibliography --- p.135

介紹.與確診多年的HIV陽性男男性接觸者（MSM）相比，新確診的HIV陽性男男性接觸者（N-MSM）傳播HIV的危險性更高，承受更多的心理健康問題，但愛滋病相關知識相對缺乏。目前的研究已經描述了N-MSM的心理健康和危險行為情況，但關於二者影響因素的調查卻甚少，且暫無關於N-MSM獨特相關因素，如疾病認知，安全套使用認知，安全使用的社會道德等的研究报告。目前，中國只有少量關於HIV陽性MSM的研究，無關於N-MSM的研究。 / 目的.本研究的目的包括： 1）描述中國N-MSM的心理健康和危險行為情況；2）驗證疾病認知量表在N-MSM人群的適應性；3）探討N-MSM疾病認知與心理健康（自殺意念，抑鬱，自我歧視和創傷後成長）的關係，並進一步研究在認知表徵與心理健康結果關係中，情感表徵的仲介作用與交互作用； 4）探索疾病認知與心理因素（抑鬱、自我歧視和創傷後成長）對自殺意念的獨立作用與交互作用；和5）研究愛滋病傳播相關危險行為的多方面因素。 / 對象與方法：本研究採用橫斷面研究設計，對中國成都共225名N-MSM進行了面對面訪談和電腦輔助移動電話訪談。根據Moss-Morris的方法，驗證疾病認知問卷的信度及效度； 使用單變量、多變量及分層的logistic和線性回歸等方法進行資料分析。 / 結果.在225名研究對象中，48%有自殺意念和抑鬱表現，35%自我歧視得分較高，17%有積極的創傷後成長。N-MSM的危險行為普遍存在：31%在確診感染後發生過非保護肛交性行為，40%認為在未來6個月意願與HIV陰性男性發生非保護肛交性行為，20%在感染後想過故意將HIV傳染給別人。經驗證，疾病認知問卷在N-MSM中具有良好的心理測量特徵。疾病認知的多個維度，如情感反應和治療控制等，與心理健康結果顯著相關。在認知表徵與心理健康結果的關係中，情感表徵表現出強烈的仲介作用。兩個疾病認知維度（情感反應與治療控制）和兩個心理因素（抑鬱和創傷後成長）對自殺意念有獨立作用，但無交互作用。影響N-MSM危險行為的多方面因素包括安全套相關認知，情感因素，心理健康和社會道德。 / 結論.本研究結果顯示中國N-MSM心理健康問題和危險行為是普遍存在的。疾病認知與心理健康結果的顯著聯繫，提示了可以通過改善N-MSM的疾病認知從而減少該人群的心理健康問題；在HIV領域中，這是一個新的研究方向。在對N-MSM危險行為的幹預研究中，需考慮影響N-MSM的獨特因素，如安全套相關認知，情感反應和社會責任等。 / Introduction.Newly diagnosed HIV positive men who have sex with men (N-MSM) comprise a special subgroup of HIV positive MSM, as they have higher risks of transmission via anal sex, suffer from more mental health problems, and possess less knowledge of HIV/AIDS than those who have been diagnosed for many years. Previous studies have investigated the prevalence of mental health and risk behaviours among N-MSMs, while few studies have explored the risk factors associated with these health-related outcomes, particularly the factors unique to N-MSMs, like illness perception, cognitions regarding condom use, social morality of condom use, etc. China provides only limited data about HIV positive MSM, and no such data is about N-MSMs. / Objectives.The aims of the study were to 1) investigate the characteristics of mental health and risk behaviours among N-MSMs in China; 2) validate the revised illness perception questionnaire (IPQ-R) modified for N-MSMs; 3) examine the effects of patients’ illness perception on their mental health outcomes and determine the mediating or moderating effects of emotional representation on the associations between cognitive representation and mental health outcomes; 4) examine the main effects and interaction effects of illness perception and psychological variables on suicidal ideation; and 5) investigate the multidimensional factors associated with risk behaviours related to HIV transmission. / Subjects and Methods.A cross-sectional study was conducted in Chengdu, China. A total of 225 N-MSMs participated in this study. Both face-to-face and computer-assisted mobile phone interviews were applied. The methodology by Moss-Morris was used to validate IPQ-R among N-MSMs. Univariate, multivariate, hierarchical logistic, and linear regression methods were used for data analysis. / Results.Of all participants, 48% had suicidal ideation and depression, and 36% reported self-stigma, but only 17% experienced posttraumatic growth (PTG). Risk behaviours were prevalent: 32% had had unprotected anal intercourse (UAI) since HIV diagnosis, 40% had the intention to have UAI with HIV negative men in the coming 6 months, and 20% had had thoughts of transmitting HIV to others purposely since HIV diagnosis. The modified IPQ-R for N-MSMs was validated and found to have acceptable psychometric properties. Most dimensions of illness perception (such as emotional response, treatment control and consequences) were significantly associated with mental health outcomes. Emotional representation had strong mediation effects rather than moderator effects on the associations between cognitive representation and mental health outcomes. Two illness perception dimensions (emotional response and treatment control) and two psychological variables (depression and PTG) showed independent effects on suicidal ideation, but not interaction effects on suicidal ideation. Multidimensional factors, such as variables related to cognitions of condom use, emotional affect (feelings of guilt regarding HIV transmission to others), mental health, and social morality (perceptions regarding responsibility for condom use), were associated with risk behaviours among N-MSMs. / Conclusions.The results show that mental health problems and risk behaviours are prevalent among Chinese N-MSM. Illness perception has a strong influence on mental health outcomes, indicating a new direction for the reduction of mental health problems via the modification of patients’ illness perception. Multidimensional factors are associated with risk behaviours related to HIV transmission, such as cognitions of condom use, emotional affection and social morality, which are unique to HIV infectors and should be considered in the development of intervention programs among N-MSMs. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Wu, Xiaobing. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2012. / Includes bibliographical references (leaves 257-276). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.i / Acknowledgements --- p.iii / List of Contents --- p.v / List of Tables --- p.xi / List of Figures --- p.xv / Abbreviations --- p.xvii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Background --- p.1 / Chapter 1.2.1 --- The importance of HIV positive men who have sex with men as a source of HIV transmission --- p.1 / Chapter 1.2.2 --- The importance of newly diagnosed HIV positive MSM (N-MSM) --- p.3 / Chapter 1.2.3 --- Knowledge gaps about N-MSM --- p.4 / Chapter 1.2.4 --- Illness perception as a relative new perceptive in HIV research --- p.5 / Chapter 1.2 --- Aims --- p.7 / Chapter 1.3 --- Structure of the thesis --- p.10 / Chapter 1.4 --- Summary --- p.15 / Chapter Chapter 2 --- Literature review --- p.16 / Chapter 2.1 --- HIV epidemic --- p.16 / Chapter 2.1.1 --- Global HIV/AIDS epidemic --- p.16 / Chapter 2.1.2 --- HIV/AIDS in China --- p.17 / Chapter 2.2 --- MSM and HIV --- p.22 / Chapter 2.2.1 --- HIV epidemic among MSM worldwide --- p.22 / Chapter 2.2.2 --- HIV prevalence and incidence among MSM in China --- p.24 / Chapter 2.2.3 --- Risk sexual behavior among HIV positive MSM and its determinants --- p.25 / Chapter 2.2.4 --- Mental health problems faced by HIV positive MSM --- p.31 / Chapter 2.2.5 --- Studies of PLWH in China --- p.38 / Chapter 2.3 --- Newly diagnosed HIV positive MSM (N-MSM) --- p.43 / Chapter 2.3.1 --- HIV transmission risk varies at different stages of HIV infection --- p.43 / Chapter 2.3.2 --- More risky sexual behavior among N-MSM --- p.45 / Chapter 2.3.3 --- More stress and lack of knowledge among N-MSM --- p.46 / Chapter 2.3.4 --- Definition of N-MSM --- p.47 / Chapter 2.3.5 --- Studies about N-MSM --- p.47 / Chapter 2.4 --- Self-regulation model and illness representation --- p.51 / Chapter 2.4.1 --- Self-regulation model --- p.51 / Chapter 2.4.2 --- Illness representation --- p.52 / Chapter 2.4.3 --- Application of illness representation in HIV research --- p.60 / Chapter 2.5 --- Audio computer-assisted self-interviewing (ACASI) --- p.61 / Chapter 2.5.1 --- Types of questionnaire deliver modes --- p.61 / Chapter 2.5.2 --- Comparisons of sexual behavior reporting between ACASI and other delivery modes --- p.63 / Chapter 2.5.3 --- Limitations of ACASI --- p.65 / Chapter Chapter 3 --- Subjects and Methods --- p.67 / Chapter 3.1 --- The study site --- p.67 / Chapter 3.2 --- Study population --- p.68 / Chapter 3.3 --- Recruitment of study participants --- p.68 / Chapter 3.4 --- Data collection procedure --- p.69 / Chapter 3.4.1 --- Face-to-face interview --- p.70 / Chapter 3.4.2 --- Computer-assisted mobile phone interview --- p.70 / Chapter 3.4.3 --- Service provision and incentives --- p.71 / Chapter 3.4.4 --- Sample size and the representativeness --- p.71 / Chapter 3.5 --- Quality control --- p.71 / Chapter 3.6 --- Establishment of the CAMP system --- p.72 / Chapter 3.7 --- Collaborator --- p.73 / Chapter 3.8 --- Measurements --- p.74 / Chapter 3.9 --- Statistical analysis --- p.83 / Chapter 3.10 --- Ethical approval --- p.85 / Chapter Chapter 4 --- Participant profiles --- p.86 / Chapter 4.1 --- Background --- p.86 / Chapter 4.2 --- Objectives --- p.87 / Chapter 4.3 --- Statistical analysis --- p.87 / Chapter 4.4 --- Results --- p.87 / Chapter 4.4.1 --- Background information --- p.87 / Chapter 4.4.2 --- Mental health outcomes --- p.88 / Chapter 4.4.3 --- HIV secondary transmission related behaviours --- p.90 / Chapter 4.4.4 --- Service utilization and quality of life --- p.94 / Chapter 4.5 --- Discussion --- p.95 / Chapter Chapter 5 --- Validation of the revised illness perception questionnaire among newly diagnosed HIV positive MSMs --- p.111 / Chapter 5.1 --- Background --- p.111 / Chapter 5.2 --- Objectives --- p.112 / Chapter 5.3 --- Modification and translation --- p.113 / Chapter 5.4 --- Statistical analysis --- p.114 / Chapter 5.5 --- Results --- p.116 / Chapter 5.5.1 --- Items of the main cognitive representation subscales --- p.116 / Chapter 5.5.2 --- Items of the emotional response subscale --- p.117 / Chapter 5.5.3 --- Items of the causal attribution subscale --- p.118 / Chapter 5.5.4 --- Examination of the identity subscale --- p.118 / Chapter 5.5.5 --- Descriptions of the derived subscales --- p.119 / Chapter 5.5.6 --- Inter-correlations among subscales --- p.119 / Chapter 5.5.7 --- External validity --- p.120 / Chapter 5.5.8 --- Associations between background variables and illness perception --- p.120 / Chapter 5.6 --- Discussion --- p.121 / Chapter Chapter 6 --- Relationships between illness perception and mental health outcomes among N-MSM --- p.132 / Chapter 6.1 --- Background --- p.132 / Chapter 6.2 --- Objectives --- p.134 / Chapter 6.3 --- Statistical analysis --- p.136 / Chapter 6.3.1 --- Analysis of the associations between illness perception and mental health outcomes --- p.136 / Chapter 6.3.2 --- Analysis of the mediation effects of emotional response on the associations between cognitive representation and health related outcomes --- p.137 / Chapter 6.3.3 --- Analysis of moderating effects of emotional response on the associations between cognitive representation and mental health outcomes --- p.139 / Chapter 6.4 --- Results --- p.140 / Chapter 6.4.1 --- Suicidal ideation --- p.140 / Chapter 6.4.2 --- Depression --- p.142 / Chapter 6.4.3 --- Self-stigma --- p.145 / Chapter 6.4.4 --- Posttraumatic growth --- p.147 / Chapter 6.5 --- Summary --- p.149 / Chapter 6.5.1 --- Associations between illness perception and health related outcomes --- p.149 / Chapter 6.5.2 --- Summary of the mediation effects of emotional response on the association between cognitive representation and health related outcomes --- p.150 / Chapter 6.5.3 --- Summary of the moderating effects of emotional response on the association between cognitive representation and health related outcomes --- p.150 / Chapter 6.6 --- Discussion --- p.151 / Chapter Chapter 7 --- The main and interaction effects of illness perception and other psychological variables on suicidal ideation among N-MSM --- p.177 / Chapter 7.1 --- Background --- p.177 / Chapter 7.2 --- Objectives and hypothesis --- p.179 / Chapter 7.3 --- Data analysis and results --- p.180 / Chapter 7.3.1 --- Associated factors of suicidal ideation in univariate analysis --- p.180 / Chapter 7.3.2 --- The associations between illness perception / psychological variables and suicidal ideation --- p.180 / Chapter 7.3.3 --- The independent effects of emotional response/treatment control and psychological variables on suicidal ideation --- p.182 / Chapter 7.3.4 --- Analysis of the interaction effects between illness perception and psychological variables onto suicidal ideation --- p.184 / Chapter 7.4 --- Discussion --- p.184 / Chapter Chapter 8 --- Risk behaviours related to HIV transmission and associated factors --- p.197 / Chapter 8.1 --- Background --- p.197 / Chapter 8.2 --- Objectives --- p.199 / Chapter 8.3 --- Statistical analysis --- p.202 / Chapter 8.4 --- Results --- p.203 / Chapter 8.4.1 --- Factors associated with having had UAI with men since HIV diagnosis --- p.203 / Chapter 8.4.2 --- Factors associated with intention to have UAI with HIV negative men in the coming 6 months --- p.205 / Chapter 8.4.3 --- Factors associated with having the thoughts of transmitting HIV to others purposively since HIV diagnosis --- p.206 / Chapter 8.5 --- Discussion --- p.207 / Chapter Chapter 9 --- Conclusions --- p.225 / Chapter 9.1 --- Summaries and interpretations of findings related to mental health --- p.225 / Chapter 9.1.1 --- Poor mental health status --- p.225 / Chapter 9.1.2 --- A validated revised illness perception questionnaire (IPQ-R) among N-MSM --- p.226 / Chapter 9.1.3 --- Strong associations between illness perception and mental health outcomes --- p.226 / Chapter 9.1.4 --- Significant associations between illness perception / other psychological variables and suicidal ideation --- p.227 / Chapter 9.2 --- Summaries and interpretations of findings related to risk behaviours --- p.228 / Chapter 9.2.1 --- Prevalent risk behaviours --- p.228 / Chapter 9.2.2 --- Factors affecting the risk behaviours related to HIV transmission and implications --- p.229 / Chapter 9.3 --- Service implications --- p.231 / Chapter 9.3.1 --- Intervention programs for reducing mental health problems --- p.231 / Chapter 9.3.2 --- Intervention programs for reducing risk behaviours related to HIV transmission --- p.233 / Chapter 9.3.3 --- Policy support in HIV prevention --- p.236 / Chapter 9.4 --- Study limitations --- p.238 / Chapter 9.5 --- Recommendations for future research --- p.239 / Chapter 9.5.1 --- Longitudinal studies --- p.239 / Chapter 9.5.2 --- Behavioural studies --- p.240 / Chapter 9.5.3 --- Intervention studies --- p.240 / Chapter 9.6 --- Conclusions --- p.241 / Chapter Appendix I --- Questionnaire for face-to-face interview --- p.243 / Chapter Appendix II --- Questionnaire for computer-assisted mobile phone interview --- p.254 / Reference --- p.257

Conclusions The results showed that depression is prevalent among Chinese PLWH and perceived discrimination is a source of stress. Perceived stress, social support and coping strategies had independent effects on mental health outcomes but there were no interaction effects. The Brief COPE was validated and had a structure different from the original, again supporting the importance of cultural variations. Our results hence provided useful instrumentations (PSSHIV and Brief COPE) as well as a good foundation for the development of theories and interventions specific to the needs of the PLWH in China. / Introduction Mental health issues are prevalent among People Living with HIV (PLWH). Instrumentation, conceptualization and theoretical models of stress-coping-mental health relationships among PLWH are important but theoretical and practical differences exist in the context of different cultures and quality of care services. / Objectives The aims of the study were 1) to develop a new Perceived Stress Scale for People Living with HIV (PSSHIV); 2) to validate the Brief COPE inventory among Chinese PLWH; 3) to investigate determinants of perceived stress and psychological well-being. / Results The newly developed PSSHIV and the rearranged version of the Brief COPE were validated and found to have acceptable psychometric properties. Of all participants, 63.3% showed moderate/severe depressive symptoms. 'Problem-solving and Acceptance' and 'Self-blame and Denial' were commonly used coping strategies. Socioeconomic status (SES), perceived discrimination, and optimism were significantly associated with overall stress and some of the subscales of PSSHIV. Perceived stress appeared to mediate the relationship between perceived discrimination and psychological well-being. In addition to perceived stress, social support and some particular coping strategies were associated with depression. Similar though not identical results were found using quality of life as the outcome. / Subject and Methods To achieve Aim 1, PLWH in two Chinese cities were interviewed for item generation and validation. Aims 2 to 4 were achieved through another survey conducted in the same cities. Participants were recruited from the registry of the Centers for Disease Control and Prevention and from users of hospital services and Methadone Maintenance Treatment clinics. Standard statistical methods were used for validation studies. / Su, Xiaoyou. / Adviser: Tak Fai Joseph Lau. / Source: Dissertation Abstracts International, Volume: 73-02, Section: B, page: . / Thesis (Ph.D.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 181-206). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

吳木欣. / "2008年7月". / "2008 nian 7 yue". / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 169-174). / Abstracts in Chinese and English. / Wu Muxin. / 目錄 / 引言 --- p.9 / Chapter 第一章 --- 文獻回顧 / Chapter 1 --- 何謂 MSM? --- p.11 / Chapter 2 --- 回顧亞洲男男性接觸者感染愛滋病之情況 --- p.11 / Chapter 3 --- 愛滋病感染率急升之原因 --- p.14 / Chapter 3.1 --- 香港同性戀者情況 --- p.17 / Chapter 4 --- 各地政府和NGOs之回應 --- p.19 / Chapter ■ --- 歧視與愛滋病的關連 --- p.20 / Chapter 4.1 --- 美國經驗´ؤ´ؤ活動取向之多元 --- p.21 / Chapter 4.2 --- 亞洲經驗一政府的不支持，非政府組織的無力 --- p.23 / Chapter 5 --- 回顧政府和香港非政府組織(NGOs)的角色 --- p.24 / Chapter 5.1 --- 香港政府採取的措施 --- p.24 / Chapter 5.1.1 --- ACA的建議政策角色 --- p.26 / Chapter 5.1.2 --- ATF的撥款機制角色 --- p.28 / Chapter 5.1.3 --- 非政府組織的工作 --- p.35 / Chapter 6 --- 是次研究的分析框架和概念 --- p.38 / Chapter 7 --- 研究重要之處 --- p.45 / Chapter 第二章 --- 研究方法 --- p.47 / Chapter 第三章 --- 誰是決策者 --- p.52 / Chapter 第四章 --- 主流組織的失敗一一反思一筆過撥款的弊端 --- p.73 / Chapter 第五章 --- 愛滋病非政府組織的無力 --- p.94 / Chapter 第六章 --- 草根組織的掙扎求存 --- p.103 / Chapter 第七章 --- 政府的割裂 --- p.115 / Chapter 第八章 --- 非政府組織的工作---愛滋病只是愛滋病嗎？ --- p.130 / Chapter 第九章 --- 總結 --- p.158 / 參考文獻 --- p.169 / 附件一 --- p.175 / 附件二 --- p.176

Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.

Ventricular dysfunction and dilated cardiomyopathy (DCM) develop among untreated HIV-infected people at much higher rates than among HIV-negative individuals, resulting in significant contributions to morbidity and mortality. Mechanisms underlying development of HIV-associated cardiomyopathy (HIVCM) are as yet poorly understood. The well-characterized simian immunodeficiency virus (SIV) model of HIV infection provides a unique context for HIVCM pathogenesis studies in that SIV-infected rhesus monkeys develop myocardial lesions and contractile dysfunction similar to those described in HIV-infected people, suggesting a shared disease mechanism. Lymphocytic myocarditis is a commonly reported finding in AIDS patients at autopsy and constitutes one of several conditions known to predispose to development of DCM, irrespective of HIV-infection status. As lymphocytic myocarditis also occurs with high frequency among SIV-infected rhesus monkeys, a retrospective analysis of rhesus monkey cardiac tissue collected at necropsy was performed to examine viral and cellular correlates of lymphocytic inflammation within myocardial tissue. One subpopulation of macrophages, which has been reported by other groups to be associated with an anti-inflammatory phenotype, was found to correlate inversely with lymphocytic infiltration and positively with numbers of virus infected cells, suggesting effects of an anti-inflammatory cytokine production profile. In contrast, the detrimental effects of inflammatory cytokines on myocardial structure and function are well-recognized and HIV infection in general is characterized by chronic immune activation and inflammatory cytokine dysregulation. To further investigate a role for myocardial cytokine production in development of HIVCM, a prospective study was conducted in which SIV-infected rhesus monkeys and uninfected controls were treated with recurrent administration of inactivated Mycobacterium aviumcomplex bacteria (MAC). SIV-infected, MAC-treated animals rapidly developed significant ventricular systolic dysfunction and chamber dilatation not seen in control groups, suggesting an exaggerated myocardial sensitivity to exogenous antigenic stimulation. Concurrent treatment with the TNFα antagonist etanercept completely abrogated development of these changes, strongly implicating a causative role for TNFα in evolution of the contractile dysfunction and chamber remodeling. Findings reported from the current studies suggest that characteristics of local myocardial macrophage populations and the myocardial tissue cytokine milieu may play more important roles than lymphocytic infiltration, cardiomyocyte damage, or viral proteins in the pathogenesis of HIVCM.