In the southern Appalachians, the dominant soil order, Ultisols, is highly susceptible to erosion. If left unmanaged these soils can develop into gully systems resulting in land degradation. This study examines gully development through sidewall mass-wasting events at a high temporal resolution using 30 minute time-lapse photography. Prior research at this site found significant mass wasting events occurring between weekly monitoring periods. By shortening the interval of observation to 30-minutes, a more accurate understanding of the frequency and intensity of these mass-wasting events, and their relation to meteorological factors, can be determined. Photographs of a gully (approximately 1.5 m deep by 3 m wide at the top) were captured every 30 minutes from 11/29/17 - 2/18/2018 with a WingScape outdoor time-lapse camera mounted on a plastic stake 3.16 m from the gully facing northwest and upstream into the gully channel. A total of n=1648 images were coded using presence/absence indices for six observed geomorphic processes: creep on NE facing sidewall, creep on SW facing sidewall, slump on NE facing sidewall, slump on SW facing sidewall, channel aggradation, and channel development. Precipitation and temperature data were collected every 5 minutes using a Davis Vantage Pro 2 weather station located 240 m from the gully, and were aggregated to various time intervals. Precipitation received in previous 0.5, 1, 1.5, 2, 3, 6, 12, 24, 36, 48, and 72 hours were calculated for each image. Two binary temperature variables were generated with values of “1” if temperature dropped below 0 °C (32 °F) during the prior 30 minutes or 24 hours, respectively, and “0” otherwise. Logistic regression models (forward conditional method) for the six geomorphic index variables were generated using the precipitation and temperature data. For creep on the NE facing sidewall, the significant independent variables are 3 hour and 72 hour prior rain, and freeze conditions in the previous 0.5 and 24 hours. On the SW facing sidewall, rain and temperature variables were also important for creep; rain in the previous 12 and 24 hours, and freeze conditions within the previous 24 hours were retained in the model. For slumping on both the NE and SW facing sidewall, recent and prolonged rain were important. Specifically, 1, 6, 12, and 24 hour rainfall were retained in both models, with the addition of 3 hour rainfall in the NE facing sidewall slump model. No temperature variables were retained. For channel aggradation (deposition of material in the channel), rain in the prior 12 and 72 hours, and freezing in the prior 24 hours were important, suggesting that freeze-thaw processes loosen the soil, and subsequent rain events carry material into the channel. When rain stops, the material is then deposited in the channel. Interestingly, no viable model could be developed for channel development (erosion) using these parameters. These results will be useful to quantify meteorological controls on gully erosion at short temporal scales.

This thesis deals with the introduction into the topic of time-lapse video creation. It focuses on cases where tripod is not used and therefore it is  necessary to eliminate incurred shortcomings. The main shortcomings are different position of individual frames, different brightness and color adjustment. The next topic describes which principles should be followed during the creation process. Thesis describes and implements methods for elimination of main shortcomings during process long time-lapse videos, which are recorded by hand. Thesis also precisely describes image registration, correction of brightness and colors. Thesis is also considers histograms comparison. Result of this work is application, which eliminates problems described above.

(v anglickém jazyce) Development of tooth primordia in mice is an important model for study of odontogenesis. Several dental rudiments develop during the mouse embryogenesis. These structures develop in functional teeth in their phylogenetically older relatives. Similarly, we can initiate growth of teeth from these germs in some mutant mice. In my diploma thesis we have focused on the importance of rudimentary structures with odontogenic potential in postnatal individuals. As a model of development, we have chosen a cell population originating from rudimentary primordia MS (mesial segment) that develops in diastema of the lower jaw during the embryonic day 12.5. Using the inducible Cre-lox technology we have marked the cells which are part of the signal domain of primordia at this time. As a marker of these cells we have used gene Shh. We have found out that these cells persist prenataly and also postnatally. Further we have isolated this cell area and we have tested it using a variety of methods. We have shown that in the cells of postnatal individual are expressed markers of stem cells (Sox2, Bmi1, Gli1) and also genes for major enamel matrix structural proteins: ameloblastin and amelogenin. The same stem cell markers are also expressed in vitro culture of the isolated cells. This cell population...

The diploma thesis deals with time-lapse electrical resistivity tomography (TL-ERT) of active slope deformations. Slope deformations represent one of the important land-forming processes. Frequently, they can cause considerable property damages and can endanger health and lives of inhabitants. Therefore, we can consider them as potentially dangerous so complete understanding of their dynamics, and their mechanisms of origin, is essential. Electrical resistivity tomography (ERT) then represents an effective geophysical tool for slope deformation investigation. Within diploma thesis, the evaluation of the several different time-series of the ERT measurements was performed. Based on findings of the one- year (August 1013 to July 2014) monitoring of resistivity changes by ERT, and also, based on daily and hourly recurrences of measured resistivity data, the optimum measuring interval has been determined, namely 12 hours. The most applicable electrode arrays and their combinations were suggested based on a testing of different electrode configurations. The results of detailed measurement with 1 m electrode spacing offered the idea of shortening of the total length of the present permanent TL-ERT profile as well as shortening the electrode spacing. These conclusions and proposed adjustments then resulted in...

Sea level rise concerns millions of people in coastal areas across the globe. One of the largest uncertainties to project future sea level rise is the frontal ablation (accounting for calving and submarine melt) at marine ice margins, around the Greenland and Antarctic Ice Sheet. High rates of frontal ablation have been observed to imply, through loss of the buttressing effect but not limited to it, increased mass loss from marine terminating glaciers and hence, associated sea level rise. This study focuses on calving processes at a freshwater lake in northern Sweden, which represents a simpler environment to study calving processes than the marine one, because impacts of tides, salinity, and circulation (all known to be relevant at marine ice-ocean boundaries) can be neglected. A multi-method approach to quantify and characterize calving events is presented here, exploring and analysing the underwater acoustic soundscape at a calving glacier front, in connection with optical, image-based methods such as time- lapse photography, and photogrammetry based on footage acquired by an uncrewed aerial vehicle (UAV). An acoustic detector is developed, tested and applied to data set acquired during 2020, and results indicate that the acoustic detector can be an important complement in the range of tools used to observe, and quantify, calving. Applied in remote locations, where continuous monitoring is difficult and where optical methods are of limited use, collecting acoustic data and monitoring calving by means of its acoustic signature could render insights previously not available (because of lacking data and methodology).

Der Tumorsuppressor p53 spielt eine wichtige Rolle bei der Aufrechterhaltung der zellulären Homöostase und verhindert die Bildung und Entwicklung von Krebs. Frühere Studien auf Einzelzellebene haben gezeigt, der p53 nach dem Auftreten von DNS Schäden in einer Serie gleichförmiger Pulse im Kern akkumuliert. Um den Einfluss von Temperatur, dem Zustand des NFκB Weges oder dem Vorhandensein von Wachstumsfaktoren auf die p53 Dynamiken nach DNS-Schäden zu untersuchen, wurden in dieser Arbeit A549 und MCF10A p53 Reporter-Zelllinien verwendet. Um Daten mit hoher zeitlicher und räumlicher Auflösung auf Einzelzellebene zu erhalten, wurde Zeitraffer-Fluoreszenzmikroskopie verwendet. Überraschenderweise zeigten A549 Zellen, die mit γ-Strahlung behandelt wurden, eine höhere p53 Akkumulation und ein verlängertes Zeitintervall zwischen den p53 Pulsen, wenn sie bei 30°C inkubiert wurden im Vergleich zu Zellen unter physiologischen Bedingungen. Hingegen zeigten Zellen bei 40°C eine höhere p53 Pulsfrequenz. Außerdem wurde die p53 Zielgen-Expression durch die Änderungen in der Dynamik beeinflusst. In den beiden Zelllinien, A549 und MCF10A, wurde die p53 Dynamik durch Inhibierung des NFκB Signalweges verändert, nicht aber durch dessen Aktivierung mittels TNF. So verlängert die Inhibierung des NFkB Signalweges das Zeitintervall zwischen p53 Pulsen, was sich auch in der Expression von p53 Zielgenen wiederspiegelt. Weiterhin konnte in MCF10A Zellen durch Experimente mit verschiedenen Medien-Bedingungen gezeigt werden, dass p53 Dynamiken vor allem durch die Anwesenheit von EGF und Hydrocortison geprägt werden. So führt EGF zu einem pulsierenden p53 Verhalten, während Hydrocortison alleine die p53 Antwort vollständig aufhebt.. Diese Studie macht deutlich, wie wichtig es ist, das Verhältnis von zellulärem Zustand und p53 Antwort systematisch zu untersuchen, um Krebstherapien wirksamer zu machen / The tumor suppressor p53 plays important roles in maintaining cellular homeostasis and in preventing the formation and development of cancer. Previous studies on p53 activation after DNA damage have reported that it shows a series of regular discrete pulses of protein accumulation over time at the single cell level. In this work A549 and MCF10A p53 reporters cell line were used to investigate how p53 dynamics after DNA damage were affected by changes in temperature, by changes in the state of the NFκB pathway and changes in the provided growth factors. Time-lapse florescent microscopy was used to obtain single cell data with high temporal and special resolution at the single cell level. Surprisingly A549 cells treated with γ-irradiation showed higher level of p53 accumulation and increased time between p53 pulses when imaged at 30°C than cells imaged under physiological conditions. Cells imaged at 40°C showed instead higher p53 pulse frequency. P53 target gene expression was also affected by these changes in dynamics. In both A549 and MCF10A cells, p53 dynamics were changed by NFκB pathways inhibition but not activation via TNFα. Upon inhibition of the NFκB pathway the timing between p53 pulses was increased leading to changes also in p53 target genes expression. In MCF10A cells experiments done under different medium conditions proved that p53 dynamic in this cell line was shaped mainly by the presence of EGF and hydrocortisone that are usual components of the media. EGF leads to a more pulsatile p53 behavior while hydrocortisone completely abrogates the p53 response. These discoveries pointed out the need to study more systematically the relationship between the p53 response to a given stress and the cellular state in order to make cancer therapies more effective.

This thesis analyses ice-rich mountain permafrost with regard to its genesis, distribution, deformation and interaction with other environmental factors. The processes influencing ground ice formation in ice-rich and ice-poor mountain permafrost are highlighted. Factors influencing the presence of ice-rich permafrost are identified and their individual or combined effect on frozen ground is determined. Based on these findings, a new permafrost distribution map of Switzerland was created, which specifies permafrost temperature and ice contents and considers rock glacier creep paths. The deformation of rock glaciers is investigated with newly developed monitoring systems and concepts. This enables a better understanding of the processes leading to rock glacier acceleration at different time scales.

This thesis analyses ice-rich mountain permafrost with regard to its genesis, distribution, deformation and interaction with other environmental factors. The processes influencing ground ice formation in ice-rich and ice-poor mountain permafrost are highlighted. Factors influencing the presence of ice-rich permafrost are identified and their individual or combined effect on frozen ground is determined. Based on these findings, a new permafrost distribution map of Switzerland was created, which specifies permafrost temperature and ice contents and considers rock glacier creep paths. The deformation of rock glaciers is investigated with newly developed monitoring systems and concepts. This enables a better understanding of the processes leading to rock glacier acceleration at different time scales.

Nous présentons dans la première partie du document une étude portant sur l'imagerie de temps de vie de fluorescence sur structures dynamiques dans le domaine de fréquence (FD FLIM). Une mesure en FD FLIM est définie par une série d'images présentant une variation d'intensité sinusoïdale. La variation d'un temps de vie se traduit par une variation dans la phase de la sinusoïde décrite par l'intensité. Notre étude comporte deux contributions principales: une modélisation du processus de formation de l'image et du bruit inhérent au système d'acquisition (capteur ICCD) ; une méthode robuste d'estimation du temps vie sur des structures mobiles et des vésicules intracellulaires. Nous présentons ensuite une étude en microscopie de fluorescence portant sur la quantification du transport hétérogène dans un environnement intracellulaire dense. Les transitions entre la diffusion Brownienne dans le cytoplasme et les transports actifs supportés par le cytosquelette sont en effet des scénarios très couramment observés dans des cellules vivantes. Nous montrons que les algorithmes classiques de suivi d'objets nécessaires dans ce contexte, ne sont pas conçus pour détecter les transitions entre ces deux types de mouvement. Nous proposons donc un nouvel algorithme, inspiré de l'algorithme u-track [Jaqaman et al., 2008], qui s'appuie sur plusieurs filtrages de Kalman adaptés à différents types de transport (Brownien, Dirigé ...), indépendamment pour chaque objet suivi. Nous illustrons sur séquences simulées et expérimentales (vimentine, virus) l'aptitude de notre algorithme à détecter des mouvements dirigés rares. / We propose in this manuscript a study of the instrumentation required for the quantification in frequency domain fluorescence lifetime imaging microscopy (FD FLIM). A FD FLIM measurement is defined as a series of images with sinusoidal intensity variations. The fluorescence lifetime is defined as the nanosecond-scale delay between excitation and emission of fluorescence. We propose two main contributions in the area: a modeling of the image process and noise introduced by the acquisition system (ICCD sensor); a robust statistical method for lifetime estimation on moving structures and intracellular vesicles. The second part presents a contribution to the tracking of multiple particles presenting heterogeneous transports in dense conditions. We focus here on the switching between confined diffusion in the cytosol and motor-mediated active transport in random directions. We show that current multiple model filtering and gating strategies fail at estimating unpredictable transitions between Brownian and directed displacements. We propose a new algorithm, based on the u-track algorithm [Jaqaman et al., 2008], based on a set of Kalman filters adapted to several motion types, for each tracked object. The algorithm has been evaluated on simulated and real data (vimentin, virus) data. We show that our method outperforms competing methods in the targeted scenario, but also on more homogeneous types of dynamics challenged by density.

Le cortex cérébral primate a subi des modifications majeures pendant l'évolution qui ont permis le développement de fonctions cognitives supérieures. Un accroissement massif a eu lieu avec l'extension spécifique des couches supragranulaires et une forte expansion tangentielle. Le cortex primate ne possède pas uniquement davantage de neurones, comparé au rongeur, mais aussi des différences qualitatives. Ceci suggère des différences qualitatives pendant le développement du cortex.Une zone proliférative corticale supplémentaire a été identifiée chez le singe macaque: la zone subventriculaire externe (OSVZ) supposée être impliquée dans l'expansion du cortex primate. Mais les propriétés des précurseurs de l'OSVZ restent mal connues. Des techniques de microscopie en temps réel et d'immunofluorescence ont permis de réaliser une description exhaustive des précurseurs de l'OSVZ et de leurs propriétés chez le singe macaque.Nos résultats mettent en évidence des différences primates/rongeurs majeures. Les observations en temps réel révèlent des capacités prolifératives bien plus importantes des précurseurs. Les précurseurs primates de l'OSVZ présentent des taux de prolifération variables pendant la corticogenèse liés à la cinétique du cycle cellulaire. Nos enregistrements ont permis la génération d'une grande base de données de propriétés et lignages de précurseurs et la mise en évidence d’une diversité morphologique inattendue. 5 types ont été identifiés. Impliqués dans des lignages complexes, chaque type a la capacité de s'auto-renouveler et de générer directement des neurones. Parallèlement, nous avons développé une méthode de classification non supervisée des précurseurs corticaux. Cette technique a identifié les mêmes 5 types de précurseurs.Les résultats de cette thèse apportent de nouveaux éléments dans la compréhension des spécificités de la corticogenèse primate qui contribuent à l'expansion corticale et au développement de capacités cognitives supérieures. / The primate cerebral cortex underwent major modifications during evolution that enabled the development of high cognitive functions. A massive enlargement occurred with the specific expansion of the supra granular layers and the apparition of new frontal areas. Not only quantitative differences are found compared to the rodent but also qualitative differences. This points to potential qualitative differences in primate cortical development. An extra proliferating zone had already been identified during macaque corticogenesis: the outer subventricular zone (OSVZ). This zone is assumed to play a key role in the expansion of the primate cortex but the cellular and functional properties of OSVZ precursors remain elusive. We used quantitative long-term time-lapse video-microscopy (TLV) and immunofluorescence in and ex vivo to perform a detailed and exhaustive description of OSVZ precursor types and proliferative abilities at different stages of macaque cortical development. Our results highlight major rodent/primate differences. TLV observations revealed a much higher proliferative potential of OSVZ compared to the rodent SVZ. We report variable rates of proliferation linked to cell-cycle duration in a stage-specific manner. TLV recordings allowed the formation of a large database of primate precursor properties and lineages. This dataset unravelled an unexpectedly high diversity of OSVZ precursor morphologies. Five precursor types were identified. Involved in complex lineages, each precursor type can self-renew and directly generate neurons. In a parallel approach, we developed an unbiased clustering tool to automatically classify cortical precursors. This technique returned the same five precursor types as the morphological categorization. The results of this PhD thesis provide new insights into primate specificities during corticogenesis that contribute to cortical expansion and to the development of higher cognitive abilities.