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Managing supply risks post pandemic : Understanding gaps in organizational decision-making and proposing a tool to manage differences / Hantering av risker i försörjningskedjan i en värld efter pandemin : Förståelse kring skillnader i beslutsfattande mellan olika organisatoriska nivåer och föreslag på att minska dessa skillnaderJoshi, Aron January 2023 (has links)
Effective supply chain risk management has become crucial for companies as supply chains have become more complex and integrated due to decades of globalization. The outbreak of the Covid-19 pandemic has highlighted the fragility of global supply chains and the necessity of manufacturing companies to have a robust supply chain in order to be resilient towards supply chain disruptions. The recent sourcing difficulties of critical components have been exacerbated due to many organizations utilizing tactical sourcing strategies which have relied on ''just in time'' principle in an effort to source as cost-efficiently as possible. However, this has created supply chains which lack the ability to manage idiosyncratic risks effectively. Supply chains which are based on the ''just in case'' principle have instead more redundancy and flexibility in the supply chain that allows them to better mitigate supply chain risk. Though the reduction in risk and strengthening of supply chain means a drop in operational efficiency. Therein lies the problem for companies when it comes to creating a supply chain; how to manage the trade-off between robustness and effectiveness. Businesses have therefore been forced to reconsider risk management strategies and practices in relation to sourcing and balancing of supply chain capabilities in order to manage disruptions in the supply chain and fluctuations in the business cycle. A case study has been conducted in order to analyze how the difference in approach towards supply chain risk management on tactical and operational level affects deployment of risk mitigation strategies in relation to supply management. The research has been conducted through a literature review and a series of semi-structured interviews at a case company involved with manufacturing. The findings from this study indicate a contrast in selection of risk mitigation strategies between those operating at tactical and operational level. Differences in approach between these groups in regard to decision-making therefore affects deployment of risk mitigation strategies. This may result in ineffective risk management as mitigation strategies might ultimately become negated and prove ineffective on an aggregated level due to conflict with decisions made on the tactical and operational level. In an effort to minimize differences which exit in approach towards risk mitigation in the organizational structure, a tool has been developed which allows for the comparison of various mitigation strategies and their impact on performance. Thereby helping guide businesses on which mitigation strategies to pursue in a way that there is minimal contradiction through focusing on addressing risks that have positive dependence rather than a negative one. Whilst also aiming to narrow the gap between in how differing decision levels perceive risk and risk management. / Effektive riskhantering av försörjningskedjor har blivit allt viktigare för företag då försörjningskedjor har ökat i komplexitet och blivit alltmer integrerade genom årtionden av globalisering. Utbrottet av Covid-19 har demonstrerat hur pass känsliga globala försörjningskedjor är samt behovet av tillverkningsföretag att utveckla robusta försörjningskedjor som kan motstå störningar. Svårigheter med inköp av kritiska komponenter har förvärrats av att många företag använt sig av taktiska inköpsstrategier vilka förlitar sig på ''precis i tid'' principen, som strävar att hantera inköp så pass kostnadseffektivt som möjligt. Dock har det skapat försörjningskedjor som saknar förmågan att hantera idiosynkratiska risker effektivt. Försörjningskedjor som istället använder sig av ''utifall att'' principen har större redundans och flexibilitet som gör att dessa försörjningskedjor kan mitigera risker mer effektivt. Dock leder den ökade riskreducerings förmågan till att den operationella effektiviteten reduceras. Däri ligger svårigheten för företag att skapa försörjningskedjor, hur hanterar man önskan om att ha operationell effektiva och robusta försörjningskedjor. Företag måste därför utvärdera sina riskhanterings- och inköpsstrategier i förhållande till hantering av försörjningskedjor samt balansering av försörjningskedjans förmågor så att störningar i försörjningskedjor och variationer i affärscykeln kan hanteras. En fallstudie har genomförts för att analysera hur skillnaden i tillvägagångssätt i förhållande till riskhantering av försörjningskedjor mellan den taktisk och operationella nivån i en organisation påverkar användandet av riskreducerande strategier i relation till säkerställande av material. Forskningen har genomförts genom en litteraturstudie samt en serie av semistrukturerade intervjuer hos ett tillverkningsföretag som deltagit i fallstudien. Resultaten i denna rapport indikerar att det råder en kontrast i val av riskreducerande strategier mellan de i den taktisk och operationella nivån i en organisation. Skillnader i tillvägagångssätt mellan dessa grupper i förhållande till beslutsfattande påverkar valet av riskreducerande strategier. Detta kan leda till ineffektiv riskhantering då det finns en chans att valda riskreducerande strategier kan motverkar varandra och därigenom bli verkningslösa på en aggregerad nivå på grund av besluts som fattats på den taktiska och operationella nivån står i kontrast till varandra. I ett försök att minska skillnader som existerat i den organisatoriska strukturen i förhållande till riskreducerande strategier så har ett verktyg utvecklats som möjliggör en jämförelse av flera riskreducerande strategier samt deras påverkan på förmågan. Därmed kan det hjälpa företag att välja riskreducerande strategier så att de inte motarbetar varandra genom att fokusera på risker som är positivt korrelerade istället för negativt korrelerade. Medans också minska gapet mellan hur olika beslutsfattande nivåer förhåller sig till risk och riskhantering.
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Identifying Synergies and Trade-offs between the Grand Ethiopian Renaissance Dam and the Sustainable Development Goals / Identifiering av Synergier och Målkonflikter mellan Grand Ethiopian Renaissance Dam och de Globala MålenThengius, Sandra, Preston, Olivia January 2018 (has links)
The Grand Ethiopian Renaissance Dam (GERD) is a large hydropower project currently under construction in Ethiopia. Supposedly it will benefit the people of Ethiopia through an increased access to electricity, but it is also associated with complex political relations between Ethiopia and the neighbouring countries Egypt and Sudan. The aim of this thesis is to analyse the GERD project with regard to the United Nations’ 17 Sustainable Development Goals and their 169 related targets, and to show what actions should be taken to improve the contribution of the GERD to sustainable development. A method developed for mapping synergies and trade-offs between targets in the SDGs was used. To identify how the GERD contributes to and/or hinders the fulfillment of the goals, published evidence was systematically searched. 83 synergies, 45 trade-offs and 16 instances when cooperation in the Nile River basin is needed were identified. By radically increasing the electricity access in Ethiopia and because of the dam itself, the GERD both potentially contributes to and hinders sustainable development in relation to environment, society and economy. Identified key actions to reinforce the synergies and minimise the trade-offs are to establish a secure and including cooperation between Ethiopia, Egypt and Sudan; to ensure increased transparency, public access to information and participatory processes; to investigate and carry out measures that benefit the ecology in the area; and to strengthen the positive outcomes of electrification through supplementary measures. / Grand Ethiopian Renaissance Dam (GERD) är ett stort vattenkraftprojekt som är under uppbyggnad i Etiopien. Genom att radikalt öka tillgången till elektricitet förväntas GERD bidra positivt till det etiopiska samhället, men dammen är också associerad med komplicerade politiska relationer mellan Etiopien och grannländerna Egypten och Sudan. Målet med rapporten är att analysera GERD-projektet i förhållande till FN:s 17 Globala mål och dess 169 tillhörande delmål, samt att visa vilka åtgärder som bör vidtas för att GERD i större utsträckning ska bidra till en hållbar utveckling. En metod som utvecklats för att kartlägga synergier och målkonflikter mellan olika delmål av de Globala målen användes. För att undersöka hur GERD bidrar till och/eller förhindrar uppfyllandet av delmålen gjordes en systematisk sökning av publicerad litteratur. 83 synergier, 45 målkonflikter och 16 fall där dessa hänger ihop med att samarbete krävs i regionen identifierades. Genom att öka tillgången till elektricitet, och på grund av dammen i sig, potentiellt både bidrar till och förhindrar GERD hållbar utveckling i förhållande till miljö, samhälle och ekonomi. Identifierade nyckelåtgärder för att förstärka synergierna och minimera målkonflikterna är att etablera ett bestående samarbete mellan Etiopien, Egypten och Sudan; att säkerställa ökad transparens, deltagandeprocesser och allmän tillgång till information; att utreda och utföra åtgärder som är till nytta för områdets ekologi; och att förstärka de positiva resultaten av elektrifiering genom kompletterande åtgärder.
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Trade-offs and Temporal Variation in Predator-Mediated Natural Selection and Sexual Selection on the Wings of the Damselfly Calopteryx splendensBoarman, McKaila J. S. 13 June 2017 (has links)
No description available.
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Phenotypic variation in host quality of pines for the European pine sawfly (Neodiprion sertifer)Chorbadjian, Rodrigo A. January 2008 (has links)
No description available.
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Consistency of demographic trade-offs across 13 (sub)tropical forestsKambach, Stephan, Condit, Richard, Aguilar, Salomon, Bruelheide, Helge, Bunyavejchewin, Sarayudh, Chang-Yang, Chia-Hao, Chen, Yu-Yun, Chuyong, George, Davies, Stuart J., Ediriweera, Sisira, Ewango, Corneille E.N., Fernando, Edwino S., Gunatilleke, Nimal, Gunatilleke, Savitri, Hubbell, Stephen P., Itoh, Akira, Kenfack, David, Kiratiprayoon, Somboon, Lin, Yi-Ching, Makana, Jean-Remy, Mohamad, Mohizah Bt., Pongpattananurak, Nantachai, Perez, Rolando, Rodriguez, Lillian Jennifer V., Sun, I-Fang, Tan, Sylvester, Thomas, Duncan, Thompson, Jill, Uriarte, Maria, Valencia, Renato, Wirth, Christian, Wright, S. Joseph, Wu, Shu-Hui, Yamakura, Takuo, Yao, Tze Leong, Zimmerman, Jess, Rüger, Nadja 04 January 2024 (has links)
1. Organisms of all species must balance their allocation to growth, survival and
recruitment. Among tree species, evolution has resulted in different life-history
strategies for partitioning resources to these key demographic processes.Life-history strategies in tropical forests have often been shown to align along
a trade-off between fast growth and high survival, that is, the well-known
fast–slow continuum. In addition, an orthogonal trade-off has been proposed
between tall stature—resulting from fast growth and high survival— and recruit-
ment success, that is, a stature−recruitment trade-off. However, it is not clear
whether these two independent dimensions of life-history variation structure
tropical forests worldwide.
2. We used data from 13 large-scale and long-term tropical forest monitoring plots
in three continents to explore the principal trade-offs in annual growth, sur-
vival and recruitment as well as tree stature. These forests included relatively
undisturbed forests as well as typhoon-disturbed forests. Life-history variation
in 12 forests was structured by two orthogonal trade-offs, the growth−survival
trade-off and the stature−recruitment trade- off. Pairwise Procrustes analysis
revealed a high similarity of demographic relationships among forests. The small
deviations were related to differences between African and Asian plots.
3. Synthesis. The fast–slow continuum and tree stature are two independent di-
mensions structuring many, but not all tropical tree communities. Our discovery
of the consistency of demographic trade-offs and life-history strategies across
different forest types from three continents substantially improves our ability to
predict tropical forest dynamics worldwide.
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Modélisations des mécanismes généraux d'assemblage des communautés pour simuler la dynamique spatio-temporelle de la biodiversité benthique / Models of general community assembly mechanisms simulating the spatial and temporal dynamics of benthic biodiversityAlexandridis, Nikolaos 28 March 2017 (has links)
Les macroinvertébrés benthiques entretiennent un ensemble complexe d’interactions. Les échelles spatiales et temporelles des processus formant la base de ces interactions ont traditionnellement limité leur étude empirique. Le premier chapitre du manuscrit tente une revue des outils de modélisation utilisés dans l’étude du benthos marin.Même si l’implémentation d’un modèle mécaniste semble s’ajuster aux communautés benthiques, son utilisation nécessite la création d’un nombre limité d’entités avec un rôle fonctionnel clair. Le second chapitre du manuscrit utilise l’hypothèse des groupes émergents, afin de faire cela via une procédure objective et testable. Le groupement est testé face aux postulats théoriques et les résultats supportent sa capacité à reproduire la diversité fonctionnelle dans l’estuaire de la Rance.Le manque de connaissances dans l’attribution des relations entre les composantes fonctionnelles reste important. Le troisième chapitre du manuscrit s’inscrit dans ce besoin, basé sur des théories écologiques qui prévoient l’existence de trade-offs fonctionnels opérant à grande et petite échelle. Dans un premier temps, ces éléments sont incorporés dans des modèles qualitatifs des groupes fonctionnels.Malgré l’intérêt du développement et de l’analyse de modèles qualitatifs, le but d’étudier la dynamique et le comportement spatialement explicite de la biodiversité ne peut être atteint que par un modèle avec ces mêmes caractéristiques. Le quatrième chapitre du manuscrit présente l’architecture d’un modèle individu-centré, en mettant l’accent sur le transfert des règles d’interactions des modèles qualitatifs vers un cadre dynamique et spatialement explicite. / Benthic macroinvertebrates are part of a complex network of interactions. The spatial and temporal scales of the processes that form the basis for these interactions have traditionally restricted their empirical investigation. The first chapter of the manuscript attempts a review of the modelling tools that have been employed for the study of the marine benthos.The implementation of a mechanistic modelling framework seems fitting, but it requires the derivation of a few model entities with a clear functional role. The second chapter of the manuscript employs the emergent group hypothesis to do that in a way that is objective and testable. The resulting grouping is tested against theoretical expectations and the results support its ability to represent functional diversity in the Rance estuary.The lack of knowledge for the attribution of relationships among functional components is still important. The third chapter of the manuscript addresses this issue based on ecological theories that predict the existence of functional trade-offs operating at both large and small spatial scales. In a first inception of the system, these elements are incorporated in the form of general rules of interaction into qualitative models of the functional groups.In spite of the interest in developing and analysing qualitative models, the goal of studying the dynamic and spatially explicit behaviour of benthic biodiversity can only be reached by a model with the same characteristics. The fourth chapter of the manuscript presents the architecture of an individual-based model, primarily transferring the rules of interaction from the qualitative models to a dynamic and spatially explicit framework.
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Causes of adaptive differences in age-dependent reproductive effortHouslay, Thomas M. January 2014 (has links)
Sexually selected ornaments are among the most spectacular traits in nature. Indeed, the extreme costs associated with producing sexual traits seem to play a crucial role in their evolution by enforcing honest levels of advertisement: only males with high levels of acquired resources (or high ‘condition’, as it is known in the literature) can afford to produce extravagant signals, a phenomenon which maintains signal reliability in a constant environment. In my thesis I examine many implications of this condition-dependent model of ornament and preference evolution for variation in age-dependent allocation to sexual signals and other life history traits. In Chapter 1, I review theoretical implications of condition-dependent signalling for life history and sexual selection theory. I note that a universal cost of expenditure in sexual advertisement is metabolic in nature: metabolites used to fund ornament expression are by definition unavailable to other life history traits that compete for a limited resource pool. This universal constraint on expenditure does more than maintain honesty (as noted above), however: the reliance of sexual displays on high levels of nutrient acquisition may help maintain genetic variation in sexual signals that would otherwise be eroded by strong mate choice, and without which the selective basis for good-genes choice would disappear. Three mechanisms in particular probably help to maintain genetic variation in acquisition. 1) Because acquiring resources and converting them efficiently to useful forms depends on the high function of many biochemical pathways, condition is undoubtedly highly polygenic, which slows the erosion of genetic variation under strong directional selection by females (especially in the presence of epistatic interactions). 2) The highly polygenic nature of condition also presents a large target for mutation, which continually restores variation at the loci under selection. 3) The many loci underlying condition may also be particularly sensitive to environmental heterogeneity in time or space. By favouring the most ornate males, females acquire high performing genes for their offspring, regardless of the precise allele combinations that have conferred the ability to acquire resources. Selection on specific alleles is liable to fluctuate over time or space whenever allelic performance is strongly context-specific. I close by noting the considerable challenges in advancing research on sexual selection and life history allocation, including the fact that two key processes central to life history (acquisition and allocation) are latent variables that interact in complexways and are intrinsically difficult to measure empirically. In the remainder of my thesis I conduct a series of experiments involving decorated crickets, Gryllodes sigillatus, which are useful models for studying life history because they enable precise measurement of male reproductive effort. Male G. sigillatus face important allocation decisions owing to the highly polyandrous nature of females, and the substantial costs involved in signalling and mating. Chapter 2 examines sex differences in age-dependent reproductive effort as a function of diet and development stage. I reared outbred crickets using four combinations of diet nutritional quality, and studied the effects of these combinations on male and female reproductive effort (calling effort in males and fecundity in females) and longevity. While I expected males to be more sensitive than females to variation in diet and developmental changes in its quality, I actually observed the opposite: males in all treatments increased calling effort over time, exhibiting consistently positive covariance between calling effort and longevity across treatments. By contrast, the relationships between female reproductive effort and longevity changed dramatically across treatments, and females who lived to intermediate ages had the highest fecundity. Although my results support sex-specific selection on life history allocation over time, a compelling additional explanation for my findings relates to the strategic role of calling for achieving male fitness. In the absence of positive feedback from potential mates, perhaps male allocation to sexual advertisement is careful and only increases gradually as a function of accumulating metabolic resources and increasing risk of intrinsic mortality. Alleles underlying condition are expected to be particularly sensitive to environmental heterogeneity. While this sensitivity may help maintain additive variation in male quality (which is essential for the sustenance of adaptive good-genes mate choice, as noted in Chapter 1), too much environmental sensitivity could also underiii mine the signal value of the male trait. For example, if there are strong genotypeby- environment interactions (GEIs) for sexual advertisement, in a rapidly changing environment females risk favouring a male whose alleles are no longer best suited to current conditions. This problem is particularly pressing for animals like crickets where males exhibit a behaviourally plastic sexual display (such as calling), and so may dynamically adjust signalling effort over time. In Chapter 3, I used inbred lines of decorated crickets to quantify age and diet dependent genetic variation in male signalling. I demonstrate that while genetic correlations across diets were quite strong for morphological traits, correlations between measures of the male sexual trait rapidly approached zero as I increased the distance in time (i.e., across widely spaced ages) or diet (i.e., comparing more dissimilar dietary histories) between samples. While extrapolating from my laboratory experiments to nature is difficult, my findings nevertheless cast doubt on the value of behaviourally dynamic signals (such as cricket calls) for reliably indicating genetic quality in realistically complex environments. In Chapter 4 I used physiological assays to evaluate factors affecting metabolite storage and use over time in decorated crickets. I manipulated the acquisition ability of all males using artificial diets that varied linearly in nutrient quality, and manipulated access to female mates over the course of the second week of adult life. By sacrificing crickets at key stages before and after manipulating the diet and social environment, I was able to estimate changes in stored metabolites, and relate these changes to calling effort and longevity. During the first week of adulthood (in the absence of females), higher diet quality significantly increased calling effort and storage of lipid, glycogen, and carbohydrate (but not protein). The presence of females increased both the probability of calling and the amount of calling during the second week, whereas diet quality only improved calling effort. By the end of the second week, calling effort had decreased, even by high quality males in the presence of females, suggesting a depletion of resources. Furthermore, the loss of condition during week 2 covaried with calling effort during the previous week irrespective of diet. Males who started the second week in high condition lost more glycogen and carbohydrate than rivals; meanwhile, lipid accumulation covaried positively with calling effort during week 2. The contrasting patterns of storage and use for lipids compared to the ‘quick-release’ metabolites (glycogen and carbohydrates) affirms starkly distinct functions for the different storage components, and underlines the importance of specific physiological measures in life history research. Finally, in the general discussion, I attempt to synthesise my thesis’s contributions to the study of life history trade-offs involving behavioural sexual displays.
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Unintended Consequences of Strategies Implemented in Canadian Healthcare Organizations to Reduce Wait Times for Elective Hip and Knee SurgeriesSabogal, Juan Carlos 12 1900 (has links)
Introduction: En réponse aux exigences du gouvernement fédéral en ce qui concerne les temps d'attente pour les chirurgies électives d’hanche et du genou, les Organismes Canadiens de santé ont adopté des stratégies de gestion pour les listes d'attente. Cependant, il n'existe pas actuellement aucune information disponible concernant les effets imprévus, positive ou négative, de ces stratégies.
Méthodologie: Un modèle qui a été construit est tombé en panne la gestion de la chirurgie d’hanche et du genou en différentes étapes, afin d'identifier les effets imprévus possibles pour chaque étape; le modèle a été validé auprès d'un panel d'experts. Cette étude a choisi quatre études de cas en fonction de leur durabilité: un cas qui a été durable, un cas qui a été modérément durable, et deux cas peu probable d'être durable. Dans cette étude qualitative, nous avons mené 31 entretiens semi-structurés entre Novembre 2010 et Juin 2011 avec les gestionnaires, les infirmières, les thérapeutes et les chirurgiens impliqués dans la gestion des stratégies du temps d’attente pour les chirurgies électives d’hanche et du genou. Les quatre cas ont été sélectionnés à partir de trois provinces / régions. Nous avons analysé les conséquences non intentionnelles aux niveaux systémique et organisationnelle en utilisant les stratégies dans chaque contexte.
Enregistrements des entrevues ont été transcrits mot à mot et soumis à l'analyse du cadre.
Résultats: Les effets négatifs sont la précarité des stratégies en raison du non-récurrente financement, l'anxiété chez les patients qui ne sont pas prêts pour la chirurgie, une redistribution du temps de chirurgie vers l’orthopédie au détriment des autres interventions chirurgicales, tensions entre les chirurgiens et entre les orthopédistes et anesthésistes, et la pression sur le personnel dans le bloc opératoire et postopératoire.
Conclusion: La stratégie d’implémentation aux niveaux national et local devrait prendre en compte les conséquences potentielles, positives et négatives. Il y a des conséquences inattendues à chaque niveau de l'organisation des soins de santé. Individuellement et collectivement, ces conséquences peuvent positivement et négativement affecter les résultats. Par conséquent, la planification de la santé doit analyser et prendre en compte les conséquences inattendues en termes de bonnes résultats inattendues, compromis et les conséquences négatives afin d'améliorer les résultats. / Introduction: In response to federal government requirements regarding wait times for elective hip and knee surgeries, Canadian healthcare organizations have adopted wait list management strategies. However, there is currently no information available regarding the unanticipated effects, positive or negative, of these strategies.
Methodology: A model was constructed that broke down the management of elective hip and knee surgery into different steps, in order to identify the unanticipated potential effects for each step; the model was validated with a panel of experts. This study chose four case studies based on their sustainability: one case that was sustainable, one case that was moderately sustainable, and two cases considered unlikely to be sustainable. In this qualitative study, we conducted 31 semi-structured interviews between November 2010 and June 2011 with managers, nurses, therapists and surgeons involved in wait time management strategies for hip and knee surgeries. The four cases were selected from three provinces/areas. We analyzed potential unintended consequences at the systemic and organizational levels of using these strategies in each setting. Interview recordings were transcribed verbatim and subjected to framework analysis.
Results: Negative effects were the strategies’ precariousness due to non-recurrent funding, anxiety in patients not ready for surgery, a redistribution of surgical time toward orthopaedics at the expense of other surgeries, tensions between surgeons and between orthopaedic surgeons and anaesthesiologists, and significant pressure on personnel in the operating suite and in post-operative care.
Conclusions: Strategy implementation at the national and local levels should take into consideration any potential consequences, positive and negative. There are unintended consequences at each level of healthcare organization. Individually and jointly, these consequences can positively and negatively affect outcomes. Therefore, health planning should analyze and take into account unintended consequences in terms of serendipities, trade-offs and negative consequences in order to improve results.
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Restoring the Mississippi River Delta in Louisiana Ecological Tradeoffs and Barriers to ActionMaulhardt, Alison 18 December 2015 (has links)
This study investigates the Louisiana 2012 Coastal Master Plan’s ability to reconcile conflicting economic and ecological demands on coastal resources. The Louisiana Coastal Master Plan was unique in combining flood control and coastal restoration under one authority. However, the objectives of flood control and coastal restoration can be in conflict. The plan was also unique in its approach of restoration from a working coast perspective. However, the objectives of ecological restoration and economic productivity do not always agree. By conducting semi-structured interviews with major coastal stakeholders, this research will explore how the planning process has accommodated the views and values of key stakeholder parties. This research aims to make more transparent the inherent environmental tradeoffs of restoration from a working coast perspective. A working coast is a compromise between economic and environmental stakeholder needs. The approach requires a balance of power to ensure that the projects selected best serve the needs of all parties. The study found that while there is industry buy in, mechanisms for mitigating economic externalities is lacking in the plan, corporate infrastructure benefits while wildlife resources are in decline.
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Evolution expérimentale et spécialisation dans le paysage adaptatif d'un gradient environnemental / Experimental evolution and specialization in the adaptive landscape of an environmental gradientHarmand, Noémie 21 June 2017 (has links)
De nos jours plus que jamais, il est nécessaire d’anticiper et de comprendre les réponses évolutives des organismes vivants, face à des habitats instables et hétérogènes. Mais à quel point cela est-il possible ? Reproduire l’ensemble du déroulé d’une trajectoire évolutive nécessite de pouvoir décrire, d’une part, le « matériel » disponible pour s’adapter (c’est-à-dire les effets phénotypiques associés à la variabilité génétique produite), d’autre part, comment agissent les forces évolutives, associées à un contexte écologique, pour aboutir à un certain « assemblage » de ce matériel. Dans sa version la plus simple, ce processus évolutif peut-être décrit par plusieurs cycles d’évènements de mutations-sélection conduisant à l’adaptation d’une population à son environnement. Cette dynamique correspond assez bien à celle qui est décrite par les populations bactériennes dans les expériences d’évolution contrôlées en laboratoire. Parallèlement, les modèles de paysages adaptatifs (phénotypiques), et en particulier le modèle géométrique de Fisher, sont des outils très puissants pour formuler des prédictions générales et quantitativement testables sur ces trajectoires évolutives. Cependant, ils restent très théoriques et ont été largement pensés dans un contexte écologique simplifié. Au cours de cette thèse, nous avons identifié les déterminants (mutationnels et sélectifs) des trajectoires évolutives à long terme de populations bactériennes s’adaptant dans différents contextes environnementaux. Une première partie des résultats est mise en lumière par la validation expérimentale et la reconstruction de la topographie du paysage adaptatif généré par différentes doses d’un antibiotique, le long d’un gradient. Une deuxième partie expérimentale vise à intégrer une composante biotique (une autre bactérie) à ce même contexte environnemental. Les processus évolutifs intervenant au cours d’une coévolution à long terme maintenue par sélection fréquence-dépendante, y sont étudiés. / Today more than ever, it is crucial to anticipate and understand the evolutionary responses of living organisms faced with heterogeneous and unstable habitats. But to what extent is this possible? To reproduce an entire evolutionary trajectory, we must first describe the “material” available for adaptation (e.g. the phenotypic effects associated with the existing and novel genetic variability), and second describe the way evolutionary forces, shaped by the ecological context, result in specific “assemblies” of this material. At its simplest, this evolutionary process can be described by several cycles of mutation-selection events, leading to the adaptation of a population to an environment. This process is reflected in the evolutionary trajectories of bacterial lineages undergoing controlled experimental evolution in the lab. Concurrently, adaptive (phenotypic) landscape models, and especially Fisher’s geometrical model of adaptation, are powerful tools to formulate general predictions, which can then be tested on such evolutionary trajectories. However, they remain highly theoretical, and are widely conceived in a simple ecological context. In this thesis, we identified the (mutational and selective) determinants of the evolutionary trajectories of bacterial lines adapting to various environmental contexts. A first set of results regards evolution along a gradient of antibiotic doses, and their relevance is highlighted by experimental validation and by the reconstruction of the underlying adaptive landscape. A second experimental part integrates a biotic component (another bacteria) to the same environmental context. The evolutionary processes acting throughout the resulting long-term coevolution – maintained by frequency-dependent selection – are studied.
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