The DrrAB proteins of Streptomyces peucetius belong to the ABC family of ubiquitous membrane transporters. The DrrA and DrrB proteins together form a drug efflux pump that carries out the transport of the anticancer drug doxorubicin by carrying out ATP hydrolysis. The present study is the first where the intrinsic factors involved in the assembly of the DrrAB functional complex have been elucidated. The drrA and drrB genes in the wild type operon have overlapping stop and start codons (ATGA) which indicates translational coupling between the two genes. On insertion of a fortuitous stop codon in DrrA it was shown that the expression of DrrB is coupled to that of the upstream gene drrA. Furthermore, it was observed that a functional complex could be achieved only when the genes were maintained in cis in a translationally coupled manner. Translational regulation in DrrA was found to be involved in the control of optimal levels of DrrB. Inhibitory interactions within drrA sequence were speculated to cause translational arrest at the C terminus of DrrA. A novel assembly domain that forms the interface between DrrA containing the Nucleotide Binding Domain (NBD) and DrrB comprising the TransMembrane Domain (TMD) was found. Based on the data presented in this study a model is proposed for the biogenesis of the DrrAB drug pump. The model suggests that translational coupling between DrrA and DrrB is crucial for functional complex formation. Further, there is evidence of regulation of translation by attenuation in the intergenic region of drrA and drrB. The regulation seems to involve the last 30 nucleotides of the mRNA of drrA and some upstream sequences within drrA that cause translational arrest within the C terminus of DrrA. Since DrrB is translationally coupled to drrA, this translational arrest in conjunction with coupling causes lowering in the levels of DrrB. Finally, since the DrrA-DrrB interaction domain lies in the C terminus of DrrA, only the fully translated DrrA product will be competent to form a complex with DrrB. This interaction between the C terminus of DrrA and the N terminus of DrrB may be crucial for initial targeting of the complex to the membrane. The model is expected to serve as primer and open up an interesting yet insufficiently understood subject of membrane protein biogenesis.

This thesis is a study of Hamilton P. Bee and his role in the American Civil War. Bee was first a Texas brigadier general, in charge of a state militia district, and then a Confederate brigadier general, in command of the Western Sub-District of Texas. His duties included administration, diplomacy, and combat. As commander of the area from San Antonio to the Rio Grande, Bee had to cope with Unionist sentiment, regulation of trade – especially the control and impressment of cotton -- border unrest, relations with Mexico and France, and Federal invasion of the Rio Grande border and occupation of the coast. He also took part in the Confederate action against Nathaniel Banks's Red River Campaign into Louisiana in 1864 and participated in the battles of Mansfield, Pleasant Hill, and Monett's Ferry. Bee proved to be a competent administrator and an excellent diplomat, but no combat general. He ran his sub-district and the cotton trade about as well as anyone could have, and through his arrangements with the cotton owners was the only one who arrived at a viable method whereby the government obtained the needed cotton. His extradition agreement with the Mexicans and his dealings with them kept the peace on the border. But he had no experience in combat, and, when the Union forces invaded Texas at Brownsville, he panicked and ran. His actions in the battles in Louisiana showed him to be courageous and level-headed in the heat of battle, but were still indicative of his inexperience. After Richard Taylor accused him of letting Banks escape and relieved him of duty, Bee could do little to regain public confidence even though he was not at fault. He spent the remainder of the conflict in Texas and at war's end fled to Mexico to escape Unionist wrath. Since Bee was involved in all the major issues of the Trans-Mississippi, a study of his career offers a key to understanding the problems and difficulties in Texas and the failure of the area to live up to its potential to aid the Confederate war effort.

In 1949, Taiwan and Mainland China were segregated from each other and governed separately. Because of closed society and blocked information at that time, even though the two were very close to each other, there was no such trans-border crime ever happens. Following China¡¦s reforms and opening up of its policies in 1978, the number of displaced workers and jobless populace increased due to a series of reforms on economical systems and nation-operated business. The transformation of economic system had caused a big impact to politics and society of Mainland China, which successfully changed the control mechanism of the government on its society, causing social changes and restructuring. The gap between rich and poverty, urban and rural, was getting bigger. Day by day, jobless issue was getting more critical. Thus, trans-border crimes arose. Taiwan declared the ending of Martial Law on July 15, 1987. And in November of that same year, Taiwan opened up the visitations to Mainland China, Taiwanese investments in Mainland China, Cross-straits educations, interactions of barter trading, agriculture and technological personnel, making the interflow between Taiwan and Mainland China closer than ever. In virtue of similar consanguinity, culture, languages and customs, many people of Mainland China are yearned towards the wealthy and comfortable living in Taiwan. As the enactments of Taiwan and Mainland China are not well-integrated and that the politics of both are opposing to each other, common framework and control against crimes are hard to establish. With the continuous crackdown actions on crimes of Taiwan in 1984, mafias and gangsters took advantage of the non-extendable jurisdiction and absconded to Mainland China. They collaborated with the local illicit dealers and continued to engage with illegal affairs as to seek for extravagant profits. Trans-border crimes for the main purpose of economic benefits then came into existence. Most types of trans-border crimes between Taiwan and Mainland China are: illegal immigration, drug smuggling, firearm smuggling, prostitution out of fraud marriages, illegal working, absconding and hiding of criminals, the kidnapping in Taiwan and paying ransom in Mainland China, swindling over the cell phones. These crimes have become new issues which influence the public securities between the cross-straits. Due to the opposing condition of the cross-straits politics, discussion and controls about the affairs remain standstill up to now. There is no proper control strategy and systemized solution can be followed to resolve the criminal issues caused by the interflow between the cross-straits. Now that the government is opening the tourists from Mainland China to Taiwan, to prevent them from engaging in crimes after ¡§jumping out of the plane¡¨ and leaving tourist team, the governments of both borders must temporarily put aside their supremacy and dispute over unification issue. Instead, they must thoroughly think over the matter, discuss and develop specific practicable schemes against trans-border crimes. The main purpose of this research is to analyze the causes, purposes, types, methodology and severity of trans-border crimes of the cross-straits. The possible mode of cooperation on public security between the two borders is proposed. In order to maintain the public order and economic progress of the cross-straits, and to protect the lives and wealth safety of the people, both borders should confer with each other and enter agreement of cooperation on criminal jurisdictions.

Thèse (M.Sc.)--Université Laval, 2008. / Bibliogr.: f. 89-101. Publié aussi en version électronique dans la Collection Mémoires et thèses électroniques.

Thesis (Ph. D.)--University of Missouri--Columbia, 2001. / "December 2001." Typescript. Vita. Includes bibliographical references (leaves 228-272). Also available on the Internet.

The persistence of water conflicts in many arid regions is not simply a matter of water shortages, but rather the lack of equitable agreements that govern the allocation of disputed water resources to mitigate the adverse impacts of hostility and resentment. As such, equity is at the heart of many trans-boundary water disputes. Mindful of the dynamics and implications of inequitable water allocation on inter-state relationships and overall regional stability, this research aims at eliciting and developing theoretical criteria for equitable distribution of water (process equity) responsible for creating equitable outcomes and perception. This research makes the distinction between “process equity” and “outcome equity” and their impact on attaining and sustaining water security, peace, and hydro-stability. These parameters of equitable processes will be developed through a review of current literature addressing the issues of water equity in arid regions, coupled with case study analyses and cross-case comparisons and semi-structured interviews of key water negotiators. These key cases will be selected through a systematic screening methodology that analyzes nine pertinent cases. Collectively, employing these methods will yield in-depth analysis and findings applicable to other international water dispute cases in the context of arid regions. Proposing an alternative strategy that views water as a catalyst for peace and cooperation rather than conflict and altercation, this research further advocates for the development and adoption of an interdisciplinary, transformative approach to conflict resolution to advance water disputes to plausible and implementable agreements. Aiming to inform the theory and practice of hydro-diplomacy along disputed water resources, this approach encapsulates three key components, including rules of engagement, mechanisms of engagement, and neutral third-party mediation. Water satiety is identified as a major characteristic of equitable water allocation agreements that ensure the level of satisfaction of all involved stakeholders and the extent to which acceptable agreements, durable implementation, and sustainable relationships among co-riparians are attained and maintained. / text

The Rim Rock country, on the western edge of a vast Trans-Pecos Texas lava field, received thousands of feet of volcanic material during the Tertiary Period. Although pyroclastic rocks predominate, several flow rocks form the most distinctive stratigraphic markers. Dikes, sills, and laccoliths were emplaced following the cessation of extrusive igneous activity. The Vieja Group, comprising the oldest volcanic strata, has been tentatively assigned to the Chadronian Stage (Lower Oligocene). Late Tertiary block-faulting created an intermontane area which subsequently received thick bolson deposits. Recent climatic fluctuations have resulted in the development of gravel-capped terraces along the Rio Grande. / text

Within the next decade an inter provincial trail, called the Trans Canada Trail, will be developed to accommodate non-motorized traffic such as hikers, skiers, horsemen, and cyclists. Anticipating the need for rest stop facilities, this thesis seeks to explore the design potential of two isolated facilities. The focus of this exploration is the impact of landscape on the development of architectural form. While existing conditions found in each site were recorded and considered throughout the design process, a conscious effort was also made to build a site rather than site plan This position, first articulated by William Rees Morrish in his book Civilizing Terrain. acknowledges that not every piece of land can become an urban place. Instead, placemaking often requires willful acts of change in order to enhance or more effectively reveal its existing qualities. This position opposes the current attitude fostered by the environmental movement that all landscapes should remain untouched. The two sites selected were both located in western Canada: one on the eastern shore of Lake in southern British Columbia and the other in a farmer's field near Milk River in southern Alberta. This thesis traces the discoveries and attempts made to locate a center, develop an arrival sequence and insert an architectural form into the experience of the landscape. Both sites were explored simultaneously, reaching a similar level of resolution, at which point the British Columbia site was dropped and the prairie site was developed further. The Alberta site then became the developed body of the thesis.

In this study 21 trans-identified youth in Toronto and Ottawa, Ontario, Canada were interviewed about their experiences related to transition. Using the materialist research strategy of Institutional Ethnography, I explore the organization of trans-specific health care services in Ontario. I describe challenges participants had in relation to accessing care and describe key differences in how care is currently delivered in Ontario. The ways in which various politico-legal and medical forms of organization shape the provision of trans care in Ontario are explored in detail. This work provides an empirically grounded addition to the growing literature that seeks to make sense of trans marginalization and exclusion.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal