Darbo tikslas: Išanalizuoti sergamumą oro-lašinėmis infekcijomis Kauno mieste 2002 – 2007 metais ir nustatyti sąsajas tarp sergamumo oro – lašinėmis infekcijomis ir imunoprofilaktikos. Darbo uždaviniai: 1. Įvertinti oro - lašinių infekcijų (tymai, epideminis parotitas, skarlatina, raudonukė, virusinis meningitas, infekcinsu meningokokas, vėjaraupiai) pasiskirstymą įvairiose amžiaus grupėse Kauno mieste 2002-2007 metais. 2. Palyginti sergamumo kai kuriomis oro- lašinėmis infekcijomis kitimą Kauno mieste 2002 – 2007 metais. 3. Nustatyti sąsajas tarp sergamumo oro – lašinėmis infekcijomis ir imunoprofilaktikos. Metodika: Informacija apie sergamumą oro-lašinėmis infekcijomis gauta iš Kauno visuomenės sveikatos centro. Duomenys apie sergamumą oro-lašinėmis infekcijomis paimti iš Kauno visuomenės sveikatos centro kompiuterinės duomenų bazės ULISAS (užkrečiamųjų ligų informacijos ir statistinės apskaitos sistema). Taip pat buvo naudojamasi 2002-2007 metų mėnesinėmis, pusmetinėmis ir metinėmis atskaitomis. Duomenys apdoroti ir išanalizuoti naudojantis statistine programa SPSS 16. Skaičiuotas vidurkis, SD, PI. Duomenys pateikiami absoliučiais skaičiais ir procentais. Buvo skaičiuojami sergamumo rodikliai 10 000 gyventojų. Rezultatai: Sergamumo pagal amžiaus grupes analizė parodė, kad rečiausiai sirgo 13 – 18 metų vaikai, o dažniausiai – 3-6 metų. Susirgimai vėjaraupiais sudaro didžiausią dalį visų oro – lašinių infekcijų - 80,1 proc. (be ūmių virusinių kvėpavimo takų infekcijų ir... [toliau žr. visą tekstą] / Aim of the study. To analyse morbidity of air-born infections in Kaunas city in 2002–2007 and to assess links between morbidity of air-born infections and immunoprophylaxis. Tasks of the study: 1. To assess prevalence of air-born infections (measles, mumps, scarlatina, rubella, viral meningitis, infectious meningococcus, varicella) in various groups of age in 2002–2007 in Kaunas city. 2. To compare trends of some air-born infections in Kaunas city in 2002–2007. 3. To assess links between air-born infections’ morbidity and immunoprophylaxis. Method: Information about morbidity of air-born infections is got from Kaunas Public Health Center. Data of air-born infections’ morbidity is taken from data basis ULISAS (a system of communicable diseases information and statistical accounting). Also, the monthly, half-yearly and annual records of the years 2002–2007 were used. Data was archived and analyzed by using the statistical programme SPSS 16. An average, SD, PI were counted. Results are submitted in total numbers and percents. Morbidity was calculated to 10000 population. Results: The analysis according to the age groups showed, that children of 13–18 years old were ill most rarely and most frequently – children of 13–16 years old. Ailment of chickenpox makes the biggest part of air-born infections – 81 % (without Acute upper airway infections and influenza), ailments of rubella were registered least – 0,2 %. Of 10698 ill cases, 67 diseases (0,63 %) were registered after... [to full text]

In 1990 the Department of National Health and Population Development of South Africa launched a nationwide immunisation coverage campaign targetted mainly at measles. In order to measure the effect of the campaign on vaccination coverage rates for children pre- and post- campaign vaccination coverage surveys were performed using a modified EPI technique, stratified for race and urban/rural residence. The results in Natal/KwaZulu showed no significant changes in vaccination coverage rates as documented by Road-to-Health cards for any race, although the trend was towards a slight increase. The results bring into question the effectiveness of immunisation campaigns as a strategy for raising vaccination coverage levels, and having a sustained impact on the incidence of measles. Alternative strategies, such as the strengthening and expansion of existing primary health care services, and changes to the immunisation schedule for measles, should be considered. / Thesis (MMed.)-University of Natal, Durban, 1992.

Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are the most prevalent pathogens to induce mastitis. The pathogenesis of infections induced by E. coli is sophisticatedly modulated by lipopolysaccharide (LPS), LPS binding protein, membrane CD14 (mCD14), and soluble CD14 (sCD14). In the first study, administration of recombinant bovine sCD14 (rbosCDl4) significantly reduced the fatality of LPS challenged mice and the severity of mouse mastitis in terms of clinical signs, bacterial load, and TNF-alpha production. Before investigating the potential of this strategy in dairy cows, endogenous sCD14 in milk was characterized. Based on the data of 396 quarters, the milk concentration of sCD14 was 6.67 +/- 0.44 mug/ml. The stages of lactation affected the concentration of sCD14 in milk, which was higher in transitional milk (0--4 days postpartum). Milk sCD14 also increased during an intramammary LPS challenge, which paralleled with SCC increase. The protective effect of sCD14 on bovine E. coli mastitis was then investigated. It was shown that rbosCDl4 sensitized the mammary gland to recruit leukocytes in response to LPS. To prove that the early recruitment of leukocytes plays a role in preventing intramammary E. coli infections, E. coli mastitis was induced in 9 dairy cows with or without 100 mug rbosCD14. Quarters challenged with E. coli plus rbosCD14 had a more rapid recruitment of neutrophils, a faster clearance of bacteria, reduced concentrations of TNF-alpha and IL-8 in milk, and reduced clinical symptoms than quarters injected with saline. / For S. aureus mastitis, a newly designed trivalent whole-cell vaccine being composed of the most dominant serotypes (T5, T8, and T336) was evaluated. The vaccine was immunized with or without either one of the two adjuvants, aluminum hydroxide (ALUM) and Freund's incomplete adjuvant (FICA). The vaccine, with or without the presence of adjuvants, increased antigen-specific IgG1, IgG2, but not IgM, in serum. However, all formulations only had limited effects on lymphocyte subsets, interferon (IFN)-gamma mRNA expression, and neutrophil phagocytosis in comparison with the control. / Taken together, the results indicated that increasing the concentration of sCD14 in milk might be a potential strategy to prevent or reduce severity of E. coli mastitis. On the other hand, both ALUM and FICA did not augment the immune responses when formulated with trivalent vaccine. A more immunostimulatory adjuvant will be required to improve the efficacy of the novel trivalent vaccine against S. aureus mastitis.

BACKGROUND: According to the Centers for Disease Control and Prevention and the National Immunization Program, there is an increase in the re-emergence of past diseases. Even with mandatory vaccination practices in the United States, there are still a number of cases of vaccine-preventable diseases (VPDs) reported yearly. It is speculated that the re-emergence of VPDs is in part due to the increase in international travel as well as the influx of immigrants. One particular group of interest includes the Hispanic migrants coming from Central and South America where some of these diseases are endemic. OBJECTIVE: The purpose of this paper is to determine the extent of VPD cases in the border state of Arizona that may be attributed to Hispanic migrant influx using data from the MMWR: Summary of Notifiable Diseases reports for the United States and the ADHS data from all Arizona counties. RESULTS: Since 1995, rates of hepatitis B and pertussis have been increasing in Arizona and have become higher for non-Hispanics than Hispanics. In 2005, hepatitis B rates were 1.53* for the United States and 7.31* for Arizona; pertussis rates were 8.72* for the United States and 21.60* for Arizona. CONCLUSION: The results of this study's analysis show the need to improve immunization efforts within the non-Hispanic populations in all Arizona counties. (*Per 100,000 population)

JESSICA MASTRODOMENICO An Examination of the Socio-Demographic Characteristics Associated with Adult Vaccination Prevalence for Preventable Diseases in the United States Background: An estimated 50,000 adults in the United States (U.S.) die each year from one of 10 vaccine preventable diseases. For those who survive vaccine preventable infections, health care costs and loss of income become more significant. While children in the U.S. aged 0-2 exhibit vaccine prevalence rates of almost 90%, some adult vaccine prevalence rates in the U.S. population are reported to be nearly 30-40% less than the goals set forth by Healthy People 2010. The purpose of this study was to examine the associations between socio-demographic characteristics of U.S. adults and adult vaccination prevalence for pneumococcal, hepatitis A, hepatitis B, tetanus, and pertussis. Methods: Data from the 2008 National Health Interview Survey were assessed examining various health indicators and characteristics of non-institutionalized adults and children. The sample was restricted to adults ≥18 years of age. Odds ratios were calculated and multivariate logistic regression was also conducted. P-values of <0.05 and 95% confidence intervals were used to determine statistical significance. Results: There were 21781 total observations; 19.3% received the pneumococcal vaccine, 9.4% received the hepatitis A vaccine, 27.2% received the hepatitis B vaccine, 55.1% received the tetanus vaccine, and 15.2% received the pertussis vaccine. Of the socio-demographic characteristics examined, age, health insurance, marital status, and education were significant for either all five or at least four of the vaccines included in this study. As one might expect those who reported health insurance and those who had a higher level of education usually had a higher likelihood of vaccine receipt as compared to those without health insurance and those with less than a high school education. Age associations varied due to age-related recommendations for certain vaccines such as pneumococcal (recommended for adults ≥65). Compared to the married population (referent), marital status results varied, but for reasons unclear. Whites, the referent group, were the most likely to be vaccinated as compared to Blacks, Hispanics/Latinos, and Asians. Hispanics/Latinos typically had the lowest likelihood of vaccination in this examination. Conclusions: This study further explores the impact of socio-demographic disparities on vaccination status and adds new information to the literature regarding adult vaccination rates for preventable diseases. While research exists related to strengthening interventions such as patient reminder systems, those who do not see the same health care providers on a regular basis remain at risk for lower vaccination prevalence. It is important to better understand the role of social determinants of health, specifically in terms of vaccinations. Future research is needed to further characterize the association of socio-demographic factors with receipt of optional vaccines in adults.

Les virus Hendra (HeV) et Nipah (NiV) sont des virus émergents appartenant à la famille des Paramyxovirus et au genre des Hénipavirus. Chaque année, ils sont responsables de nombreuses épidémies touchant plusieurs espèces animales dont les hommes, avec une forte morbidité et mortalité. À ce jour, aucun vaccin ni traitement ne sont commercialisés. Ce projet porte sur le développement d'un vaccin génétique pour lutter contre une infection par les Hénipavirus. La stratégie suivie, repose sur l'injection in vivo de vecteurs recombinants dérivés du virus Adéno-Associé (AAVr) codant pour la glycoprotéine d'enveloppe G du virus NiV. Une première expérience réalisée chez la souris, a montré qu'une seule injection de vecteurs AAVr par voie IM permet le développement d'une réponse humorale contre la protéine G, forte et stable dans le temps. Afin de tester le pouvoir protecteur de ce vaccin, des hamsters ont été infectés par les Hénipavirus, compte tenu de leur grande sensibilité à ces infections. L'injection de vecteurs AAVr chez ces animaux a permis de protéger 100 % des animaux infectés par le virus NiV et 50 % des animaux infectés par le virus HeV. Cette étude apporte une nouvelle approche de vaccination et de nouvelles perspectives concernant l'utilisation des vecteurs AAVr pour lutter contre des infections virales émergentes.

La méningite épidémique est un problème de santé publique historiquement ancré dans la société nigérienne. Il y a plus d'un siècle que le phénomène est signalé au Niger, souvent considéré comme l'épicentre d'un espace appelé " ceinture de la méningite ". Au 21ème siècle les épidémies de méningite deviennent des moments de grande mobilisation politique et scientifique par les grands dérangements qu'elles occasionnent (plus de 3000 morts en 1995). Le caractère massif et la rapidité d'évolution de la maladie condamnant à mort le malade à peine 24 heures après son apparition, en fait l'une des affections les plus redoutées. Au début des années 1960, en envoyant un médecin militaire français, Léon Lapeyssonnie au chevet du Niger, l'Organisation Mondiale de la Santé a voulu faire de la méningite une des maladies transmissibles majeures à contrôler. En dégageant les séquences temporelles des épidémies de méningite sur un demi siècle, Léon Lapeyssonnie aura réussi à faire de la méningite non seulement "un problème spécifiquement africain", mais aussi une maladie tropicalisée, c'est-à-dire cantonnée au sein d'un espace écologique coupable s'étendant de l'ouest à l'est de l'Afrique et comprenant des coordonnées géographiques précises qui permettent d'associer les épidémies à un espace social. Une anthropologie du temps présent montre que le phénomène a évolué et que d'importants enjeux de santé publique, sociaux, politiques entourent la question du contrôle de cette maladie au Niger. Dominé par le paradigme de l'épidémiologie, le gouvernement des épidémies de méningite est émaillé de fortes controverses scientifiques depuis plus d'une décennie alors qu'on note aujourd'hui un tournant dans l'épidémiologie de la maladie. Que peut nous apprendre l'anthropologie sur la construction d'un phénomène pathologique aussi complexe que la méningite qui lie écologie, médecine et société? Cette thèse au croisement de l'anthropologie de la santé et de la sociologie des sciences, se propose de voir de quelle manière, la méningite a été construite comme un problème de santé publique au Niger. Je m'intéresse particulièrement à ce qui apparaît comme un paradoxe : la gestion d'une " maladie courante " comme un problème urgent. J'étudie ce phénomène sur trois terrains spécifiques : un laboratoire scientifique, un comité de gestion épidémique et une zone rurale en milieu haoussa.

Many mathematical models generally consist of either a continuous system like that of a system of differential equations, or a discrete system such as a discrete game theoretic model; however, there exist phenomena in which neither modeling approach alone is sufficient for capturing the behaviour of the intended real world system. This leads to the need to explore the use of combinations of such discrete and continuous processes, namely the use of mathematical modeling with what are known as hybrid dynamical systems. In what follows, we provide a blueprint for one approach to study several classes of equilibrium problems in non-equilibrium states through the direct use of hybrid dynamical systems. The motivation of our work stems from the fact that the real world is rarely, if ever, in a state of perfect equilibrium and that the behaviour of equilibrium problems in non-equilibrium states is just as complex and interesting (if not more so) than standard equilibrium solutions. Our approach consists of an association of classes of traffic equilibrium problems, noncooperative games, minimization problems, and complementarity problems to a class of hybrid dynamical system called projected dynamical systems. The purposed connection between equilibrium problems and projected dynamical system is made possible through mutual connections to the robust framework of variational inequalities. The results of our work include theoretical contributions such as showing how evolution solutions (non-equilibrium solutions) can be analyzed from a theoretical point of view and how they relate to equilibrium solutions; computational methods for tracking and visualizing evolution solutions and the development of numerical algorithms for simulation; and applications such as the effect of population vaccination decisions in the spread of infectious disease, dynamic traffic networks, dynamic vaccination games, and nonsmooth electrical circuits.

Francisella tularensis is one of a small group of bacteria recognized for their virulence and potential for use as biological weapons. In this study we utilize a novel approach to identify an immunologically prominent component of F. tularensis that appears to be a promising vaccine candidate. Francisella is an intracellular pathogen that infects cells of the reticuloendothelial system. Other bacteria, such as Brucella spp. have this part of their life cylce in common. However, while mice injected with Brucella spp. survive and produce antibodies to the bacteria which are immunologically reactive not only with Brucella spp. but, also with Francisella. When we vaccinated mice with a B. abortis O-linked polysaccharide (OPS) and then challenged them with 10 LD50F.tularensis LVS, 60% survived. Sera from Brucella OPS-primed/F.tularensis-challenged mice was used to identify immune reactive proteins from F. tularensis. A novel 52 kDa fraction was identified. While vaccination of mice with this partially purified fraction only provided 20% protection to F.tularensis challenged mice, both whole cell extracts and a partially purified soluble fraction (>30kDa) given to Brucella-vaccinated mice were 100% protective. The 52 kDa enriched fraction elicited a rudimentary cytokine burst of nitric oxide in a cell culture of J774.1 macrophages. The 52 kDa fraction was degraded by proteinase K and appeared to decrease in size to 36 kDa in the presence of DNAase, suggesting a possible protein and nucleic acid composition. The host response to F. tularensiss infection is complex, but given the ability of the 52 kDa component to protect against live vaccine challenge, and its apparent ability to elicit a cytokine burst, this component may have potential use in future vaccine production. / xii, 97 leaves ; 29 cm.

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