Memoria para optar al título profesional de Ingeniero Agrónomo / Los bajos niveles de cuajado en cranberries han motivado el estudio de distintos aspectos relacionados con la biología reproductiva de la especie. Dichos estudios indican que el factor limitante que induciría estos bajos niveles de cuajado, sería la disponibilidad de carbohidratos durante el período que abarca desde inicio de floración al cuajado. El objetivo de este estudio fue evaluar el efecto del sombreamiento, y de aplicaciones de sacarosa en cinco distintos estados de floración sobre el cuajado y el rendimiento del cranberry var. Stevens e identificar el periodo en que la disponibilidad de carbohidratos es limitante en el cuajado de frutos. Con este fin se realizaron mediciones de carga frutal, rendimiento, repartición de biomasa vegetativa y calidad de frutos y jugo en una plantación adulta de cranberry en la Región de la Araucanía. Los resultados permitieron identificar un periodo común en que la aplicación de sombreamiento afectó negativamente el cuajado y el rendimiento del cranberry, mientras que la aplicación de sacarosa afectó positivamente estas variables. Este período corresponde al estado de final de plena flor e inicios de cuajado de frutos. La distribución de materia seca no mostró diferencias significativas para ninguno de los dos ensayos, mientras que las variables de calidad de fruta (peso individual y distribución de calibres) y la intensidad colorante del jugo solamente fueron afectadas en el caso del ensayo de sombreamiento. Se concluye que el período de fines de plena flor - inicios de cuajado de frutos, corresponde a un período crítico para el cultivo del cranberry var. “Stevens” durante el cual se determina la carga frutal de la planta. Bajo las condiciones del presente estudio, las aplicaciones de sacarosa durante dicho período aumentaron el cuajado y el rendimiento de este frutal sin afectar la calidad de los frutos y del jugo. / The purpose of this research was to analyze the effect of shading and sucrose sprays at five different flowering stages of the cranberry cultivar “Stevens” on fruit set and yield. Additionally the study aimed at identifying the period during which the plant’s carbohydrate availability is a limiting factor for fruit set. Fruit set, yield, vegetative biomass as well as fruit and juice quality measurements were conducted on a cranberry farm in the Araucanía Region of Chile. A common period during which fruit set and yield were negatively affected by shading and positively affected by sucrose application was identified. This period corresponded to end of full bloom and onset of fruits. Vegetative biomass distribution was not affected by neither shading nor sucrose applications whereas quality traits such as individual fruit weight and size distribution, as well as juice color intensity were only affected by shading. The period during which the plant carbohydrate status is crucial could be identified and, under the trial’s conditions, sucrose sprays during this period enhance fruit seat and yield of cranberry cv. Stevens without affecting fruit and juice quality.

Cranberry tipworm, Dasineura oxycoccana Johnson (a gall‐making fly), disrupts normal growth of cranberry (Vaccinium macrocarpon Aiton) by injuring the apical meristem of shoots or uprights. The impact of larval feeding injury on reproductive parameters of cranberry was determined, from one growing season to next, at upright (Maine and Massachusetts, 2008 ‐ 2009) and plot levels (Massachusetts, 2009 – 2010 and 2010 ‐ 2011). I also estimated the proportions of uprights injured because of tipworm feeding at several cranberry production sites (Massachusetts and Maine) and the proportions of uprights that produced flowers and fruits in the next growing season. Tipworm‐injured uprights tagged at the end of the growing season did not produce floral‐units (following year) across sites in both Massachusetts and Maine. There was significant variation among the sampled sites in the proportions of tipworm‐injured uprights and also in the proportions of uprights with flowers in the next growing season (Massachusetts and Maine). A trend was apparent wherein sites with higher tipworm injury levels had relatively lower flowering proportions in the next growing season. However, sites in Massachusetts did not differ in the proportions of uprights that set fruit and in a replicated study, significant reduction in tipworm injury at plot level (using insecticide) did not impact flower and fruit production in the next growing season. A two‐year field study was carried out at three different locations to determine the impact of tipworm feeding injury on the reproductive and vegetative growth of two cranberry cultivars (‘Howes’ and ‘Stevens’) in Massachusetts. Individual uprights of cranberry exhibited tolerance to natural (tipworm) and simulated apical meristem injury in the current growing season (fruit production) and results were corroborated by a greenhouse study. In the field study, weight of fruit was higher intipworm‐injured uprights as compared with intact control uprights at the sites with Howes. However, majority of injured uprights (tipworm and simulated) did not produce new growth from lateral buds (side‐shoots) before the onset of dormancy. In the next growing season, fewer injured uprights resumed growth and produced flowers as compared with intact uprights at two of the three sites.

Ph.D.

Nosocomial, or hospital acquired, infections, are ubiquitous within the modern clinical setting leading to over $5 billion annually of related healthcare costs in North America. All indwelling devices are highly susceptible to bacterial colonization where physico-chemical interactions between bacteria and biomaterial surfaces have been implicated as determinant factors in the fate of the initial adhesion processes. It has been proposed that by exploiting interference strategies within this critical step of infection the ability to create 'non-infective' biomaterials may be developed. / This thesis demonstrates the effectivity of North American cranberry (Vaccinium macrocarpon) derived proanthocyanidins in preventing the adhesion of pathogenic bacteria to biomaterial surfaces. Specifically, using a model of catheter associated urinary tract infection, significant reductions in initial adhesion of uropathogenic Escherichia coli and Enterococcus faecalis to PVC and PTFE were observed. With the application of colloidal theory, a mechanism of steric interference was determined as responsible for these effects. / The evidence presented implicates PAC as a molecule of interest for the development of novel biomaterials with increased resistance to bacteria colonization.

The growth and yield response of strawberry (Fragaria X annassa Duch.) and cranberry (Vaccinium macrocarpon Ait) to various growth inhibitors, but especially to paclobutrazol (PP333), and fertilizers were investigated in glasshouse and field studies. PP333 decreased vegetative growth in a rate-dependent manner in both strawberry and cranberry. PP333 delayed fruit ripening in strawberry and decreased fruit set and fruit size in cranberry. PP333 decreased pollen germination of strawberry. Soil residues of PP333 were biologically active when measured 11 weeks after application in the strawberry study and when measured after 50 weeks in a cranberry study. In a field study with cranberry, PP333 caused a decrease in vegetative growth and an increase in flowering in the next season after treatment. Two-dimensional partitioning was used to account for yield variation from treatment effects on adjusted yield variates. The truss number was the most important contributor to yield variation in the strawberry, but was not affected by treatments. PP333 made a substantial contribution to yield variation in strawberry through its effect on fruit development and ripening. In the cranberry, fruit set was the major contributor to yield variation and PP333 influenced yield substantially through its effect on fruit set. In glasshouse studies, PP333 decreased shoot elongation in cranberry within 3 weeks of application, and increased the number of branches on primary shoots. Buds were formed within 7 weeks of treatment and some of the buds contained flowers. Shoot growth was greater with a high rate of NPK fertilizer than with the low fertilizer rate and was greater in peaty soil than in sandy soil. While bud set was not modified by soil type or fertilizer rate, high NPK fertilizer decreased floral induction. Some effects of PP333 treatment were decreased by gibberellic acid, but generally these effects here not reversed. In a field study with the cranberry, PP333 increased flower bud set for flowering and non-flowering uprights but slightly decreased floral induction for non-flowering uprights. / Land and Food Systems, Faculty of / Graduate

No description available.

Sanding and pruning are two practices used in the cranberry industry for vine management and yield stimulation. This study compared the effects of varying levels of sanding and pruning in April 2006 on vine canopy characteristics and yield over the course of two growing seasons. Each practice was applied at four levels: sanding at four depths: control (0 cm), light (1.5 cm), moderate (3.0 cm), or heavy (4.5 cm) of sand; pruning at four numbers of passes with a commercial pruner: control (0 passes), light (1 pass), moderate (2 passes), and heavy (3 passes). Pruning levels had no affect on upright density over the two seasons whereas heavy sanding treatment decreased the number of uprights per unit area significantly. A linear increase in light penetration was observed for the first season only as intensities increased for both pruning and sanding. Number of fruiting uprights relative to total uprights decreased in the first year as intensity increased for sanding and pruning. This effect continued in the second year for sanding treatments. Yield and net returns averaged over the two years were greatest in lightly pruned plots, followed by lightly sanded plots. Moderate and heavy treatments were associated with lower yields and net returns than those for the controls.

Thesis (M.S.B.E.)--University of South Florida, 2009. / Title from PDF of title page. Document formatted into pages; contains 108 pages. Includes bibliographical references.

As a species domesticated only in the last century, agricultural cranberry plants (Vaccinium macrocarpon) remain little removed from their wild relatives. Thus, it is a potential model species for studies of the earliest stages of domestication; however, there is little available quantitative information on its wild population biology and ecology. As such information is vital to studies of the ecological changes occurring during domestication, the purpose of this study was to consolidate the relevant knowledge available and conduct a preliminary search for patterns in the invertebrate communities of wild bogs. The alpha diversity was found to be greater than the overall (gamma diversity), which is likely a result of the metric used and the fact that there was minimal overlap in rare species between bogs. In addition, alpha diversity was found to be significantly negatively correlated with bog age. Two pairs of species were found to be correlated with each other, Blunt-nosed (Limotettix vaccinia (Van Duzee)) and Sharp-nosed leafhopper (Scaphytopius sp.), along with fleabeetle (Sysena frontalis (F.)) and firebeetle (Cryptocephalus incertus (Oliv.). In addition, it was found that spiders are significantly more common in western bogs, while firebeetle is significantly more common in eastern bogs. However, this may be indicative of a correlation with bog age rather than a true correlation with geographic location. More work is needed to determine the true dynamics driving these findings, so that the information could eventually be used to improve the efficacy and decrease the environmental impact of pest management on agricultural bogs.

Il a été établi que la majorité des plantes vasculaires abritent des micro-organismes endophytes bactériens et fongiques, qui peuvent coloniser les tissus végétaux et former des associations allant du mutualisme à la pathogénèse. Les symbioses végétales mutualistes les plus communes impliquent les champignons endo-mycorhiziens arbusculaires (AMF). Ces champignons s’associent aux racines des plantes et leur permettent d’améliorer leur nutrition minérale, tandis qu’ils bénéficient des composés produits par l'hôte. Toutefois, les plantes de la famille Ericaceae s’engagent plutôt dans des associations mutualistes avec les champignons mycorhiziens éricoïdes (ErMF). Ces derniers sont morphologiquement et taxonomiquement mal définis, en apparence distribués aléatoirement parmi les espèces issues des grandes divisions taxonomiques des Ascomycota et Basidiomycota. En raison de cette incohérence taxonomique et de l'absence d'une histoire évolutive explicative, la diversité réelle de ces champignons est mal caractérisée. De ce fait, ce projet vise à étudier le microbiote associé à la plante Ericaceae Vaccinium macrocarpon Aït (canneberge), axant la recherche sur les angles morphologiques, génomiques et transcriptomiques des champignons de type ErMF et autres endophytes capables de contrôler la croissance des agents phytopathogènes et de stimuler la croissance des plantes. Notre première démarche présentée dans le chapitre 2 s’est focalisée sur la caractérisation du microbiote endophyte bactérien et fongique de la canneberge, une plante vivace principalement produite en Amérique du Nord, notamment au Québec. Nous avons isolé et identifié 180 micro-organismes à partir de plantes de cultivars variés, collectées de champs différents, et avons démontré l'existence d'une variabilité dans le microbiote selon les tissus, les cultivars, et même entre les champs d'une même ferme. Parmi les endophytes d’intérêt identifiés, l’isolat fongique Lachnum sp. EC5 a stimulé la croissance des cultivars de canneberge Stevens et Mullica Queen et a formé des structures intracellulaires similaires à celles des ErMF à l’intérieur des cellules racinaires de la canneberge. De plus, l’isolat EB37 identifié Bacillus velezensis s’est révélé être un puissant agent antifongique, montrant cependant une tolérance particulière au champignon Lachnum sp. EC5, lors des tests de confrontation. Ce volet sera détaillé avec plus de précision dans le chapitre 4. Le chapitre 3 a porté sur l’analyse génomique comparative de l’isolat fongique Lachnum sp. EC5 avec plusieurs espèces de champignons Leotiomycetes ErMF, saprophytes et pathogènes. Nous avons analysé le sécrétome protéique prédit de ces champignons et mis en évidence que les gènes codant pour les enzymes de dégradation des parois végétales ne sont pas corrélés au mode de vie fongique (mycorhizien, pathogène ou saprophyte). A l’inverse, 10 protéines effectrices de Lachnum sp. EC5 prédites pour cibler spécifiquement un compartiment intracellulaire chez les cellules végétales ont des similarités avec celles d’espèces mutualistes comme Meliniomyces variabilis et Oidiodendron maius. Aussi, la protéine effectrice putative Zn-MP, prédite pour cibler, potentiellement, les chloroplastes végétaux nous permet de proposer un rôle dans le renforcement de l’immunité végétale. Le chapitre 4 s’est intéressé aux mécanismes de régulation d'expression de gènes induits lors de l’interaction entre le champignon Lachnum sp. EC5 et la bactérie B. velezensis EB37. Ces mécanismes ont été comparés à ceux activés chez la bactérie en présence de champignons pathogènes. Nous avons démontré une physiologique cellulaire bactérienne distincte en présence de Lachnum sp. EC5, dénotée par une faible expression des gènes induits lors du stress nutritif associé aux processus de sporulation, de formation du biofilm, de secretion de CAZymes et de lipopeptides. Nous avons suggéré que la sous-régulation de ces mécanismes serait essentiellement explicable par une disponibilité plus importante en glucose ou en d’autres sources de carbone préférentielles pour la bactérie. En réponse, le champignon Lachnum sp. EC5 a vécu différents changements morphologiques. Il aurait détoxifié ses environnements intra et extra-cellulaires et surexprimé sa voie de production de carbone dépendante du cycle du glyoxylate, générant ainsi des conditions favorisant un contact physique entre les deux micro-organismes. En conclusion, nous avons argumenté et documenté que la définition des ErMF basée uniquement sur des critères morphologiques est mal adaptée à catégoriser ces champignons. Notre approche multidisciplinaire a mis en évidence la diversité du microbiote de la canneberge, a étendu la notion d’ErMF à d'autres champignons jusqu'ici exclus de ce groupe, et a souligné l'importance des associations interspécifiques sur l’interaction ErMF-plantes. Ces avancées permettront d’améliorer nos connaissances sur le microbiote des plantes éricacées contribuant, au développement de solutions environnementales éco-responsables pour l’industrie de la canneberge. / It has been established that the majority of vascular plants harbour bacterial and fungal endophytes that colonize plant tissues, and thus form associations that range from mutualism to pathogenesis. Mycorrhizal fungi are a particular class of endophytes that associate with plant roots and enhance plant mineral uptake. The most common type of mutualistic plant symbiosis involves arbuscular mycorrhizal fungi (AMF), whereas plants of the Ericaceae family instead engage in mutualistic associations with ericoid mycorrhizal fungi (ErMF). The ErMF group, in its current definition, includes both ascomycete and basidiomycete species, yet is morphologically, taxonomically and evolutionarily poorly defined, which implies that the group’s true diversity is not well understood. The objective of this project is to complement morphological information with genomic and transcriptomic data to better understand the role of ErMF in 1) controlling the negative effects of pathogenic infections, and 2) the potential plant growth stimulation for the Ericaceous plant Vaccinium macrocarpon Ait. Our first approach presented in Chapter 2 focused on the characterization of the bacterial and fungal endo-symbiotic microbiota of the Ericaceous plant, Vaccinium macrocarpon Ait (cranberry), a perennial plant mainly in North America, particularly in Quebec. We isolated ~180 distinct bacterial and fungal endophytes collected from roots, stems, and leaves of cranberry plants cultivated in Quebec, Canada. We show that the cranberry microbiome varies substantially between tissues, cultivars, and across fields of the same farm. Among the isolated endophytes, the fungus Lachnum sp. EC5 was found to promote the growth of cranberry cultivars Stevens and Mullica Queen, and to form intracellular structures resembling those other ErMF inside the cortical root cells. In addition, the bacterium Bacillus velezensis (EB37) has been found to be a potent antifungal agent. Interestingly, a confrontation test between EB37 and the fungus Lachnum sp. EC5 revealed a mutual tolerance, which we will describe later in chapter 4. In chapter 3, our project focused on the comparative genomic analysis of the fungus Lachnum sp. EC5 with several Leotiomycete ErMF, saprophytes and pathogens. We analyzed fungal secretomes and demonstrated that genes encoding plant cell wall degradation enzymes are conserved between the tested fungi which suggests that such proteins are not indicative of a particular fungal lifestyle. On the other hand, 10 effector proteins identified in Lachnum sp. EC5 were also only found in mutualistic fungi, such as Meliniomyces variabilis, Oidiodendron maius and have been reported to target the plant intracellular compartments. Also, the identification of the putative effector protein Zn-MP, specific to Lachnum sp. EC5 and predicted to target plant chloroplasts, suggest a role in the reinforcement of plant immunity. Chapter 4 focuses on the patterns of gene expression regulation induced in the biocontrol bacterium B. velezensis EB37 in interaction with the potentially mutualistic fungus Lachnum sp. EC5. These mechanisms were then compared to those activated when the bacterium is in the presence of pathogenic/saprophytic fungi. We demonstrated that in co-culture with Lachnum sp. EC5, EB37expresses fewer genes related to stress, and fewer related to the stationary phase which often involves production of bacterial biofilms and lipopeptides, such as mycosubtilin. We suggest that the lessened response to stress is related to an increased availability of glucose or other preferential sources of carbons for the bacterium. Conversely, Lachnum sp. EC5 in the presence of EB37 underwent morphological changes by a higher lateral branching., detoxified its external and internal environment by expressing both a catalase activity and efflux pumps, and overexpressed its glyoxylate cycle-dependent carbon production pathway, and thus promoting favourable conditions for close physical contact with the bacterium. In conclusion, we demonstrated that the morphological-based definitions are poorly adapted to the categorization of ErMF fungi. Our multidisciplinary approach highlighted the diversity of the cranberry microbiota, extended the notion of ErMF to other fungi hitherto excluded from this fungal group and underlined the importance of interspecific associations on the ErMF-plant interaction. These advances enhance our understanding of the Ericaceous plant microbiota and contributes to the development of sustainable solutions for the cranberry industry.