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Toxicological Analysis of Acaricides for Varroa Mite ManagementVu, Philene Dung 15 June 2016 (has links)
The varroa mite is a primary driver behind periodical losses of honey bee colonies. The mite requires bees for food and reproduction and, in turn, elicits physiological deficiencies and diseases that compromise bee colony health. The mite nervous system is a target site for existing acaricides. These acaricides not only have adverse health effects on bees, but resistance limits their use to reduce mites and diseases in bee colonies. Voltage-gated chloride channels are involved in the maintenance of nerve and muscle excitability in arthropod pests, which suggests that these channels might be exploited as targets for acaricides. Apistan® (the pyrethroid tau-fluvalinate), Checkmite+® (the organophosphate coumaphos), and Apivar® (the formamidine amitraz) are control products for mite management. The effectiveness of these chemistries has diminished as a result of the increasing incidence resistance in mite populations. I report a toxicological analysis of stilbene products against acaricide-susceptible and -resistant mites. My results find a significant increase in metabolic detoxification enzyme activities in acaricide-resistant mites compared to susceptible mites. Acetylcholinesterase of coumaphos-resistant mites was significantly less sensitive to the toxic coumaphos metabolite compared to susceptible mites, which suggests target-site insensitivity as a mechanism of acaricide resistance. The stilbene product DIDS had significantly higher field efficacy to acaricide-resistant mites compared to Apistan®- and CheckMite+®. These data suggest that DIDS, and other stilbene products, might serve as candidate chemistries to continue field efficacy testing of alternative acaricides for Apistan® and CheckMite+® resistant mites. / Master of Science in Life Sciences
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The costs and benefits of resistance and tolerance behaviors against Varroa mite (Varroa destructor Anderson and Trueman) in honey bee (Apis mellifera L.)Bahreini, Rassol 16 December 2014 (has links)
Managed honey bee colonies face severe winter losses in northern climates. In my studies, interactions between genotypes of bees (genetically selected stock and unselected stock) with different levels of resistance and tolerance to varroa mites were assessed under a variety of treatment combinations to quantify effects of queen pheromone, acaricide treatment, wintering method, ventilation condition and pathogen infection on the costs and benefits associated with mite removal and mite-tolerance behaviors. In most of the experiments, mite-resistance caused greater varroa mite mortality within selected stock relative to unselected stock. Artificial and natural sources of queen pheromone caused greater varroa mite mortality within honey bee colonies relative to queenless colonies. While mite resistance had significant benefits, I showed that when producers selected colonies containing some mite resistance traits, it was traits associated with mite-tolerance and not mite-resistance were maintained and contributed to wintering success. Tolerance was effective at two levels of mites as obtained by late autumn treatment of colonies with oxalic but treatment did not improve wintering performance of either stock. Selected stock showed greater colony size, survival and resulted in more viable colonies in spring in comparison to unselected stock with similar initial mite levels (0.16 mites per bee). Selected stock showed greater relative wintering success than unselected stock when wintered indoors than when wintered outdoors but indoor wintering improved colony survival in both stocks relative to outdoor wintering. Carbon dioxide level increased within the winter bee cluster when colonies were maintained under restricted-ventilation (mean 3.82±0.031%, range 0.43-8.44%) and restricted ventilation increased mite mortality by 138% relative to standard-ventilation (mean 1.29±0.031%, range 0.09-5.26%), but restricted-ventilation did not affect bee mortality in comparison to standard-ventilation. In a laboratory study, I showed that Nosema inoculation (with co-infections of N. ceranae and N. apis) suppressed the effectiveness of mite removal behavior within selected bees relative to unselected bees. N. ceranae was more abundant than N. apis. Bees with greater mite removal capacities had higher costs associated with varroa-resistance as indicated by greater bee mortality rates when inoculated with varroa but bee mortality was not affected in Nosema inoculated bees.
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Apiculture and Bee Health in Central SwedenLarne, Olof January 2014 (has links)
Pollination necessary for the agricultural crop production affects the functions of the ecosystems on earth. In landscapes where wild pollinators are decreasing, honey bees promote the maintenance of plant species, therefore honey bee losses are of great concern. Current honey bee colony losses (Apis mellifera) worldwide are caused by Colony collapse disorder, the mite Varroa destructor and pesticides. This results in the honey bees weakened immune defenses making them susceptible to different diseases. Studies show that long-term natural selection for coexistence, or resistance to Varroa mites by honey bees is possible, but further developments are needed for this application in managed beekeeping. Furthermore, lactic acid bacteria found in honey bees can play a crucial role by improving its immune response. At places where apicultural practices have led to decreased amounts of lactic acid bacteria in the bees, supplementary feeding is a possible treatment solution. The beekeepers' observations of mite reproduction dynamics and the overwintering of strong and healthy honey bees are needed to decrease Varroa treatment with synthetic chemicals. Based on this knowledge, a small survey of beekeepers in Örebro County, Sweden, was conducted in an attempt to determine the status of their bees during the last 5 years. The largest colony loss over the past 5 winters was predominantly in 2012-2013. Varroa mite infestations with disease symptoms were primarily found in the central region. Since the survey was small and time was limited it was only possible to make general conclusions. Deeper understanding of lactic acid bacteria in honey bee societies and their inhibition of different diseases are important for future research.
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Evaluation of the Fungi Beauveria bassiana, Metarhizium anisopliae, and Clonostachys rosea as Bio-control Agents against the Honey Bee Parasitic Mite, Varroa destructorSinia, Alice 08 1900 (has links)
Laboratory bioassay was used to determine the pathogenicity of nine isolates of fungi of the genera Metarhizium, Beauveria and Clonostachys to the parasitic mite, Varroa destructor. All nine isolates were pathogenic to V. destructor with Metarhizuim anisopliae UAMH 9198, Clonostachys rosea UAMH 9161 and Beauveria bassiana GHA being the most pathogenic within their respective species. Metarhizium anisopliae UAMH 9198 was more lethal to V. destructor than B. bassiana GHA and C. rosea UAMH 9161 with LC50 values of 1.6 x 10(5), 9.6 x 10(6) and 5.4 x 10(6) conidia/mL, respectively. Metarhizium anisopliae and B. bassiana significantly affected brood and adult honey bee survivorship and their immune responses. They were lethal to the bees with LC50s of 3.70 x 10(6) and 2.62 x 10()5 conidia/mL, respectively. The effect of temperature and thymol on conidia germination, production and colony growth of the fungal isolates was determined. Temperature significantly affected conidia germination, production and colony growth (P < 0.05) but thymol did not. Efficacy of the two most promising isolates (M. anisopliae UAMH 9198 and B. bassiana GHA) as potential bio-control agents against V. destructor in hives was evaluated. Fungal inocula were applied as dry formulation, with corn flour as carrier, using dispenser tray and dusting applications. Treatments were either applied alone or in combination with thymol to determine any synergistic effects. All treatments significantly increased mite mortality (P < 0.05), however, the mite control efficacy varied between fungal treatments and application methods. Combined treatments of fungi and thymol caused significantly higher mite mortality than single fungal treatments, which showed control levels of ≤61%. Significant differences in mite mortality were found between the two delivery methods with the differences depending on the fungal isolate. The results suggest that M. anisopliae UAMH 9198 would be a more effective bio-control agent for the management of V. destructor in honey bee colonies than the other isolates tested when dispensed continuously in hives using delivery methods such as a dispenser tray. However, future research is needed to improve delivery methods and investigate the effect of carriers used in the formulation on the efficacy of such entomopathogenic fungi. / Ontario Ministry of Agriculture and Food (OMAF), University of Guelph
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<i>Varroa</i> mite management among small-scale beekeepers: Characterizing factors that affect IPM adoption, and exploring drone brood removal as an IPM toolWhitehead, Hannah R. 23 May 2017 (has links)
No description available.
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