The project focuses on the urban design of an urban residential block, primary school and park in the newly emerging ecological district of Plaines du Loup in Lausanne, Switzerland. The proposal is based on the created master plan and regulatory plan from the TRIBU architectes, which sets out the basic rules for building regulations and approach in the area.

3 plattforms, one railway station, tracks, park and postindustrial romaticism in tracking area. Reconstruction old railway station, old structure vs. new structure. Centrum of sustainability in the virtuality park. (moving object on the tracks, posibility for exhibitions, educations, fun and relax.

Zuzana (23:46): Project is rekonstruction of building number 24 and new building on space of building number 25 (technical condition of this building doesn´t alowed any use) in area of former bata´s factory in Zlín. Big importancy of this area mirrors in way of redefining urbanism and character of reconstruction of n.24. New building recpect this situation with own volume and heigh, but look of the building shows its new presents and high-tech character of functions inside. Project creates a space for three parts of Business centre- teaching centres for actual companies, universal spaces to rent whith laboratories and offices and science centre for Tomas Bata University. Area contains also undergroun parking, restaurant and another retail spaces. Idea is to create atractiv space for work, interaction and relax-all very important factor for difficult psychical work.

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Virtual Reality (VR) is a growing frontier of immersive consumable content. It provides an additional layer of immersion, namely presence, which can be described as the feeling of being somewhere, in this case, inside a virtual environment, thus accepting it as real. We utilise existing game design, VR, and perception models and theories to create prototypes that test the effect of realism in the representation of the player avatar on the felt presence in VR. We consider the concepts of “game feel”, “presence”, “scales of realism” and the “Uncanny Valley” theory when building the framework for this study. Furthermore, we create three prototypes with increasing levels of visual polish and three test groups (N = 36 adults) are made to play through one of the prototypes each. Galvanic skin response (GSR) is used to measure arousal response, a measure used to quantify degrees of presence. Participants are instructed to interact with two stimuli that share the same behaviours, first a virtual toy car and then a virtual spider. The instructions for the interactions with each stimulus are identical. Results from t-tests and linear mixed-effects modelling (LMM) show indications of effectiveness of increased player avatar visual polish on producing higher arousal response readings, suggesting an increased sense of presence. Additionally, some behavioural patterns that emerged suggest a heightened sense of embodiment. We argue that the findings validate the implementation of higher visual fidelity in player avatars, in pursuit of impacting a subject’s level of arousal when exposed to a virtual spider. This has implications for game designers, psychotherapists, and researchers who aim to use VR to induce an immersive state in individuals. / Virtuell verklighet (engelska: “Virtual Reality”) är ett växande område av konsumerbara innehåll som medverkar till att användaren ska kunna försvinna bort i en artificiell verklighet. Den engelska termen “immersion” är välanvänd inom speldesign och syftar inom denna kontext på ett uppslukande av användarens uppmärksamhet när de interagerar med ett datorspel. Virtuell verklighet bidrar med ytterligare ett lager av “immersion” nämligen genom närvaro (engelska: “Presence”). Närvaro hänvisar till känslan att befinna sig på en viss plats, i detta fall i en virtuell miljö. Vi har nyttjat sedan tidigare kända modeller och teorier relaterade till speldesign, virtuell verklighet och kognitiva förmågor som utgångspunkt för att utveckla tre prototyper. Målet med dessa prototyper är att testa vilken effekt en mer realistisk representation av en användares virtuella kropp (engelska: “Player Avatar”) kan ha på den upplevda närvaron i en virtuell verklighet. De tre prototyperna har avsikten att öka den visuella detaljrikedomen (engelska: “Polish”) på en skala från en abstrakt representation av en mänsklig kropp till en mer realistisk och faktisk representation. Deltagande i experimenten bestod av N = 36 vuxna individer, uppdelade i tre testgrupper. Vi använder galvanisk hudrespons (“GSR”) för att mäta fysiologiska responser, vars data vi använder för att kvantifiera olika grader av närvaro. Deltagarna är instruerade att interagera med två olika stimuli, som innehar samma beteendemönster, först en virtuell leksaksbil och sedan en virtuell spindel. Resultaten från t-testerna och de linjära modellerna med blandade effekter (engelska: “Linear Mixed-Effect Modelling” eller “LMM”) gav indikationer på att en högre visuell trofasthet av deltagarens virtuella kropp resulterade i en större fysiologisk respons vilket kan tolkas som att deltagarna upplevde en större närvaro. Vidare upptäcktes vissa upprepade beteendemönster som kan tolkas som indicier på att deltagarna upplevde en högre nivå av förkroppsligande i korrelation till de mer visuellt imponerande prototyperna. Vi lägger fram argumentet att fynden i denna studie validerar implementationen av en högre visuell trofasthet i spelarnas virtuella kroppar med syftet att påverka deltagarens fysiologiska respons när de exponeras för en virtuell spindel. Detta har implikationer för speldesigners, psykoterapeuter och forskare som eftersträvar att inducera ett sorts uppslukande tillstånd bland individer.

The IEEE 802.11 based Wireless LAN (WLAN) has become increasingly ubiquitous in recent years. However, due to the broadcast nature of wireless communication, attackers can exploit the existing vulnerabilities in IEEE 802.11 to launch various types of attacks in wireless and wired networks. This thesis presents a statistical based hybrid Intrusion Detection System (IDS) for Rogue Access Point (RAP) detection, which employs distributed monitoring devices to monitor on 802.11 link layer activities and a centralized detection module at a gateway router to achieve higher accuracy in detection of rogue devices. This detection approach is scalable, non-intrusive and does not require any specialized hardware. It is designed to utilize the existing wireless LAN infrastructure and is independent of 802.11a/b/g/n. It works on passive monitoring of wired and wireless traffic, and hence is easy to manage and maintain. In addition, this approach requires monitoring a smaller number of packets for detection as compared to other detection approaches in a heterogeneous network comprised of wireless and wired subnets. Centralized detection is done at a gateway router by differentiating wired and wireless TCP traffic using Weighted Sequential Hypothesis Testing on inter-arrival time of TCP ACK-pairs. A decentralized module takes care of detection of MAC spoofing and totally relies on 802.11 beacon frames. Detection is done through analysis of the clock skew and the Received Signal Strength (RSS) as fingerprints using a naïve Bayes classifier to detect presence of rogue APs. Analysis of the system and extensive experiments in various scenarios on a real system have proven the efficiency and accuracy of the approach with few false positives/negatives and low computational and storage overhead. / Master of Science

L’implication des protéines tyrosines phosphatases (PTPs) dans la régulation de la signalisation et la médiation des fonctions cellulaires a été bien établie dans les dernières années. Cependant, les mécanismes moléculaires par lesquels les PTPs régulent les processus fondamentaux tels que l’angiogenèse demeurent méconnus. Il a été rapporté que l’expression de la PTP DEP-1 (Density-enhanced phosphatase 1) augmente avec la densité cellulaire et corrèle avec la déphosphorylation du récepteur VEGFR2. Cette déphosphorylation contribue à l’inhibition de contact dans les cellules endothéliales à confluence et diminue l’activité du VEGFR2 en déphosphorylant spécifiquement ses résidus catalytiques Y1054/1059. De plus, la plupart des voies de signalisation en aval du VEGFR2 sont diminuées sauf la voie Src-Gab1-AKT. DEP-1 déphosphoryle la Y529 de Src et contribue à la promotion de la survie dans les cellules endothéliales. L’objectif de cette thèse est de mieux définir le rôle de DEP-1 dans la régulation de l’activité de Src et les réponses biologiques dans les cellules endothéliales. Nous avons identifié les résidus Y1311 et Y1320 dans la queue C-terminale de DEP-1 comme sites majeurs de phosphorylation en réponse au VEGF. La phosphorylation de ces résidus est requise pour l’activation de Src et médie le remodelage des jonctions cellules-cellules dépendantes de Src. Ce remodelage induit la perméabilité, l’invasion et la formation de capillaires en réponse au VEGF. Nos résultats démontrent que la phosphorylation de DEP-1 sur résidu tyrosine est requise pour diriger la spécificité de DEP-1 vers son substrat Src. Les travaux révèlent pour la première fois un rôle positif de DEP-1 sur l’induction du programme angiogénique des cellules endothéliales. En plus de la phosphorylation sur tyrosine, DEP-1 est constitutivement phosphorylé sur la thréonine 1318 situé à proximité de la Y1320 en C-terminal. Cette localisation de la T1318 suggère que ce résidu pourrait être impliqué dans la régulation de la Y1320. En effet, nous avons observé que la T1318 de DEP-1 est phosphorylée potentiellement par CK2, et que cette phosphorylation régule la phosphorylation de DEP-1 sur tyrosine et sa capacité de lier et d’activer Src. En accord avec ces résultats, nos travaux révèlent que la surexpression du mutant DEP-1 T1318A diminue le remodelage des jonctions cellules-cellules et par conséquent la perméabilité. Nos résultats suggèrent donc que la T1318 de DEP-1 constitue un nouveau mécanisme de contrôle de la phosphorylation sur tyrosine et que ceci résulte en l’activation de Src et l’induction des fonctions biologiques des cellules endothéliales en réponse au VEGF. Suite à ces travaux dans les cellules endothéliales qui démontrent un rôle positif de DEP-1 dans la médiation des réponses angiogéniques, nous avons voulu approfondir nos connaissances sur l’implication potentielle de DEP-1 dans les cellules cancéreuses où l’activité de Src est requise pour la progression tumorale. Malgré le rôle connu de DEP-1 comme suppresseur tumoral dans différents types de cancer, nous avons émis l’hypothèse que DEP-1 pourrait promouvoir les fonctions biologiques dépendantes de Src telles que la migration et l’invasion dans les cellules cancéreuses. Ainsi, nous avons observé que l’expression de DEP-1 est plus élevée dans les lignées basales de cancer du sein qui sont plus invasives comparativement aux lignées luminales peu invasives. Dans les lignées basales, DEP-1 active Src, médie la motilité cellulaire dépendante de Src et régule la localisation des protéines impliquées dans l’organisation du cytosquelette. L’analyse d’un micro-étalage de tissu a révélé que l’expression de DEP-1 est associée avec une réduction tendencielle de survie des patients. Nos résultats proposent donc, un rôle de promoteur tumoral pour DEP-1 dans la progression du cancer du sein. Les travaux présentés dans cette thèse démontrent pour la première fois que DEP-1 peut agir comme promoteur des réponses angiogéniques et du phénotype pro-invasif des lignées basales du cancer du sein probablement du à sa capacité d’activer Src. Nos résultats suggèrent ainsi que l’expression de DEP-1 pourrait contribuer à la progression tumorale et la formation de métastases. Ces découvertes laissent donc entrevoir que DEP-1 représente une nouvelle cible thérapeutique potentielle pour contrer l’angiogenèse et le développement du cancer. / The implication of protein tyrosine phosphatases (PTPs) in the regulation of cell signalling events and the mediation of cellular functions in response to growth factors such as VEGF has been well-established in the last years. Nonetheless, molecular mechanisms by which PTPs regulate fundamental processes such as angiogenesis are not well-characterized. Expression of the PTP DEP-1 (Density-enhanced phosphatase 1) was reported to increase with cell density and was associated with VEGFR2 dephosphorylation contributing to cell contact inhibition in confluent endothelial cells. We previously demonstrated that DEP-1 attenuates VEGFR2 activity by dephosphorylation of its Y1054/1059 leading to decreased activation of major signalling pathways downstream of VEGFR2 with exception of the Src-Gab1-AKT pathway. Increasing Src activity due to DEP-1-mediated dephosphorylation of its Y529 promotes endothelial cell survival. The objective of this thesis was to gain a better understanding of the role of DEP-1 in the regulation of the Src activity and of biological responses in endothelial cells. We identified tyrosine Y1311 and Y1320 in the C-terminal tail of DEP-1 as major phosphorylation sites in response to VEGF. These residues are required for Src activation and mediate the Src-dependent remodelling of endothelial cell junctions inducing permeability, invasion and capillary formation upon VEGF stimulation. We showed that VEGF-induced DEP-1 tyrosine phosphorylation directs DEP-1 specificity towards its substrate Src. Our results thus highlighted for the first time the promoting role of DEP-1 on the angiogenic program in endothelial cells. In addition to tyrosine phosphorylation, DEP-1 is constitutively phosphorylated on a threonine residue (T1318) proximal to Y1320 in its C-terminal tail suggesting it might be involved in the regulation of Y1320. Indeed, we found that DEP-1 T1318 is phosphorylated, potentially by CK2, and regulates the tyrosine phosphorylation of DEP-1 and its ability to bind to and activate Src. Consistent with this, remodelling of endothelial cell junctions and permeability are impaired in endothelial cells expressing the DEP-1 T1318 mutant. Thus, DEP-1 phosphorylation on T1318 displays a regulatory control over DEP-1 tyrosine phosphorylation and subsequently Src activation and endothelial cell functions in response to VEGF. Our results demonstrating that DEP-1 promotes angiogenic cell responses in endothelial cells, prompted us to consider a possible involvement of DEP-1 in cancer cells, where Src activation has been linked to cancer progression. Thus, although, DEP-1 is believed to act as a tumour suppressor in different cancer types, we hypothesized that it might also promote Src-dependent functions such as migration and invasion in cancer cells. Interestingly, we found that DEP-1 is higher expressed in more invasive basal-like breast cancer cells than in luminal-like cell lines. Moreover, DEP-1 is implicated in the regulation of Src activity, Src-mediated cell motility and appropriate localization of proteins mediating cytoskeletal organization in basal-like breast cancer cell lines. To further support these results, analysis of a breast cancer tissue microarray revealed that DEP-1 expression is associated with a tendency towards reduced overall survival. Thus, our results provide first evidence for a tumour-promoting role of DEP-1 in breast cancer. Altogether, the work performed in the context of this thesis revealed that DEP-1 can similarly behave as a promoter of the angiogenic response and of the pro-invasive phenotype in basal-like breast cancer cell lines, most likely due to its ability to activate Src. This suggests for the first time that DEP-1 expression could contribute to tumour progression and the formation of metastases, and as such, represent a potential new target for anti-angiogenic and anti-cancer therapy.

This diploma thesis deals with the employment of disabled people in the Czech Republic. There is also a description of foreign systems of the employment of disabled people, concretely in the Federal Republic of Germany and in the United Kingdom. After the descriptive part of individual systems, there is their comparison and the choice of working elements from abroad which could improve the Czech system. Then, the thesis includes orientation questionnaire survey, which focuses on the level of foreknowledge and orientation of HR specialists in the area of the employment of disabled people in the Czech Republic.

Abscract The topic of the master thesis is focused on the visualization of the Czech language curriculum as a foreign language. It summarizes the findings of the importance of images in the teaching process and the principles of its use. Individual chapters are devoted to didactic functions of the image, the relationship between image and text and the use of image specifics in foreign language teaching. Furthermore, presents graphical and pictorial means by which are teaching materials visualized. The work also focuses on visual media and their use in the classroom. Theoretical knowledge is applied in the practical part, which deals with research of the visual part of Czech textbooks for foreigners. Research part examines the didactic functions of the image, use of visual aids and presentation of vocabulary, grammar and cultural studies. The thesis also partially covers the issue of signaling grammar and didactic use of film clips. The thesis main focus is on practical advices and examples of how to integrate visualization elements into foreign language teaching and is intended for experts and for foreign language teachers.

Title: The game analysis of the performance of the creative players in professional football. Objectives: The aim of the work is to analyse the success of creative players in professional football. The study of the literature to find out the requirements for the exercise of creative players and we will analyze the success by the facts on the basis of indirect observations of gaming performance in the selected master games. Findings will evaluate the application and subsequently we will formulate specific recommendations for the practice. Methods: We carry out analysis method of indirect observations with the help of videos of selected events and subsequent analysis. Results: Analysis of selected game actions in the attack phase of the game in the middle of the center ofensive player, and their success. Keywords: Football, the offensive phase of the game, individual game performance, creativity in football, the disposition of the players on the field, diagnostics in football.