Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Foram conduzidos ensaios de avaliação de 17 genótipos de feijão-caupi de porte semi-ereto nos anos de 2007, 2008 e 2009, no período de julho a setembro de cada ano, com o objetivo de identificar aqueles com ampla adaptabilidade e estabilidade de produção no Estado de Roraima. O delineamento experimental utilizado foi o de Blocos Completos Casualizados, com 17 tratamentos (genótipos) e quatro repetições. Foram coletados dados de número de dias transcorridos da emergência das plantas até o início da floração, comprimento de cinco vagens, massa de cinco vagens, número de grãos de cinco vagens, peso de grãos de cinco vagens e peso de grãos na área útil da parcela, corrigida para umidade padrão de 13% e transformada para produtividade (kg ha-1). Com base nos dados coletados foram estimados o peso de 100 grãos e o índice de grãos. Os resultados foram submetidos à análise de variância individual e conjunta e, posteriormente, foram realizadas as análises de adaptabilidade e estabilidade de produção pelas metodologias propostas por: Eberhart e Russell (1966) e Annicchiarico (1992). Os melhores genótipos foram as cultivares BRS Cauamé, BRS Tumucumaque e BRS Guariba. Nenhuma linhagem experimental superou essas três cultivares quando consideradas todas as variáveis analisadas. / Trials have been conducted to evaluate 17 semi erect cowpea genotypes in the years 2007, 2008 and 2009, in the period from July to September of each year aiming identify those with wide adaptability and stability of production in Roraima State. The experimental design was a randomized complete block design with 17 treatments (genotypes) and four replications. The collected data were the number of days elapsed from plant emergence until the beginning of flowering, lodging, length of five pods, mass of five pods, number of grains of five pods, grains weight of five pods and grains weight in each plot, corrected to standard moisture of 13% and transformed to productivity (kg ha-1). Based on data colected were stimated weight of 100 grains and grain index. The results were submitted to individual and jointly analysis of variance and were later carried out the analysis of adaptability and stability of production by the methodologies proposed by Eberhart and Russel (1966) and Annicchiarico (1992). The best genotypes were BRS Cauamé, BRS Tumucumaque and BRS Guariba cultivars. No experimental lines overcame these three cultivars when considering all variables evaluated.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O feijão-caupi constitui-se uma das leguminosas de grande importância para o Estado de Roraima e seu cultivo pode ser encontrado em diversas áreas da região. É uma leguminosa que pode beneficiar da fixação biológica de nitrogênio, através da simbiose com bactérias do gênero Bradyrhizobium. Desta forma, objetivou-se neste trabalho caracterizar e avaliar a eficiência simbiótica de 41 estirpes de rizóbios isoladas de ambientes de mata e cerrado em Roraima. As estirpes foram caracterizadas morfologicamente e agrupadas de acordo com os perfis gerados, em seguida foram caracterizadas geneticamente através da amplificação e sequênciamento parcial do gene 16S rRNA. Utilizando o feijão-caupi como planta isca, foi conduzido um experimento em casa de vegetação para avaliação da eficiência simbiótica, do qual determinou-se a matéria seca da parte aérea, número e massa seca de nódulos. Deste experimento, oito estirpes que se mostraram mais eficientes foram testadas em campo. No campo dois experimentos foram conduzidos, um em área sem cultivo anterior com feijão-caupi e outro em área cultivada por dois anos. Dos experimentos de campo, aos 30 dias foram avaliados, massa seca e N-total da parte aérea, número e massa seca de nódulos e no final do ciclo determinou-se a produtividade de grãos. Do total de estirpes caracterizadas, a maioria apresentou crescimento lento (58%) e reação neutra (51 %) do meio de cultura, características que foram compatíveis com as do gênero Bradyrhizobium. A análise do sequênciamento parcial do gene 16S rRNA revelou que as estirpes obtidas de feijão-caupi apresentam elevada diversidade fenotípica e genotípica, com predominância de estirpes do gênero Bradyrhizobium e em menor proporção estirpes com características semelhantes ao gênero Rhizobium. Os resultados obtidos no ensaio de eficiência simbiótica mostrou que 20 estirpes foram eficientes, sendo todas pertencentes ao gênero Bradyrhizobium. Três estirpes (ERR 24, ERR40 e ERR 510) apresentam bons resultados para os atributos avaliados em condições de campo, apresentado valores satisfatórios quanto à eficiência simbiótica igual ou superior as estirpes atualmente recomendadas e ao controle nitrogenado, sendo indicadas como novos inoculantes. / The cowpea constitutes one of the legume plants of great importance to the State of Roraima and its cropping can be found in several areas of the region. It is a legume plant which can benefit from the biological fixation through symbiosis with bacteria of the genus Bradyrhizobium. In this way, it was aimed in this work to characterize and evaluate the symbiotic efficiency of strains of rhizobia isolated from Forest and cerrado (savannah-like vegetation) environment in Roraima. The strains were characterized morphologically and grouped together according to the profiles generated; next, they were characterized genetically through the amplification and partial sequencing of gene 16S rRNA. Utilizing the cowpea as a bait plant, an experiment was conducted in a greenhouse for evaluating of the symbiotic efficiency, from which the dry matter of the shoot, number and dry matter of nodules were determined. Of this experiment, eight strains which proved more efficient were tested in field. In the field, two experiments were conducted, one in an area with no previous cropping with cowpea and the other in an area cultivated for two years. Of the Field experiments, at 30 days, dry matter and total-N of the shoot, number and dry mass of nodules were evaluated and at the end of the cycle, grain yield was determined. Of the total of strains characterized, most of them presented slow growth (58%) and neutral reaction (51 %) of the culture medium, characteristics which were consistent with those of the genus Bradyrhizobium. The analysis of the partial sequencing of gene 16SrRNA revealed that the strains obtained of cowpea present a high phenotypic and genotypic diversity, with predominance of strains of the genus Bradyrhizobium ADN to a smaller extent strains with characteristics similar to the genus Rhizobium. The results obtained in the symbiotic efficiency assay showed that 20 strains were efficient, all of them belonging to the genus Bradyrhizobium. Three strains (ERR 24, ERR40 and ERR 510) present good results as regards the symbiotic efficiency for the attributes evaluated under field conditions, presenting satisfactory values concerning the symbiotic efficiency equal or superior to the strains currently recommended and to the nitrogen-containing control, they being recommend as new inoculants.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Objetivou-se com este trabalho avaliar a fitossociologia, o manejo da vegetação natural e sua influência agronômica do feijão caupi na savana de Roraima. / The objective of this study was to evaluate the phytosociology, the management of natural vegetation and its influence on the agronomic efficiency of cowpea in the savannah of Roraima.

MSCAGR (Plant Production) / Department of Plant Production / See the attached abstract below

M. Tech. (Department of Biotechnology, Faculty of Applied and Computer Sciences), Vaal University of Technology / Bambara groundnut (Vigna subterranea L. Verdc) is an underutilized crop in the African continent. It is a drought tolerant crop and fixes atmospheric nitrogen. Bambara groundnut is primarily grown for the protein content of its seeds and is mainly produced by small scale farmers at the subsistence level. However, despite its importance as a subsistence crop in many African countries, only local landraces of bambara groundnut are still cultivated. Mass selection of a few local varieties for the main agronomic characteristics has been carried out. All the bambara groundnut germplasm in South Africa has not been morphologically characterized. Although the protein of bambara groundnut is of good quality and is rich in lysine, there is no information on the characterisation of these proteins. The presence of antinutritional factors in the crop has also received little attention. This study focused on three major objectives including: (I) to assess the extent of morphological variations among thirty selected landraces of bambara groundnut, (II) to characterize the major seed proteins in these accessions using one dimensional gel electrophoresis, and (III) to determine the presence of any anti-nutritional factors in the seeds of the selected bambara groundnut landraces. 30 accessions of bambara groundnut were evaluated for their variability in agronomic and morphological traits. The field experiment was conducted at ARC-VOPI in Roodeplaat research farm during the 2014/2015 summer cropping season. The field trial was arranged as a complete randomized block design with 3 replications. 18 quantitative traits were recorded to estimate the level of genetic variability among accessions. 4 different methods were employed to extract seed proteins from 30 bambara groundnut accessions in order to ascertain the best method for protein extraction. These methods included: 10%-80% isopropanol, 10% trichloroacetic acid (TCA) in acetone solution, sonication and 2x Lammeli buffer extraction methods. The quick start Qubit® fluorometer protein kit was used to determine the protein concentration in each sample. The samples were then subjected to one dimensional gel electrophoresis. For antinutritional analysis, 5 factors (condensed tannins, free and phytic acid phosphate, polyphenol and trypsin contents) were used to determine the amount of antinutrient in 30 bambara seeds that were ground to a fine powdery flour. 3 replicates of all the samples were ground for each assay evaluated. The flour was then immediately extracted and used for the different assays. The analysis of variance revealed significant differences only in 10 of the 18 phenotypic traits that were evaluated. The UPGMA cluster analysis based on the quantitative traits produced vii four distinct groups of genotypes and a singleton. Genotypes SB11-1A, SB19-1A, SB12-3B and Bambara-12 were found to possess good vegetative characters and are recommended for use as suitable parents when breeding cultivars for fodder production. Desirable yield and yield-related traits were identified in B7-1, SB4-4C, SB19-1A, Bambara-12 and SB16-5A and are recommended as suitable parental lines for bambara groundnut grain production improvement. The quantitative characters therefore provided a useful measure of genetic variability among bambara genotypes and will enable the identification of potential parental materials for future breeding programmes in South Africa. Out of the 4 different seed protein extraction methods exploited for this study, the 2x Laemmli buffer extraction method produced the best result with clear protein bands. A unique feature from all extraction methods was the presence of a common protein band at ̴ 75 kDa. All extraction methods except 10 % TCA-Acetone resolved common banding patterns in all the bambara groundnut samples. This data suggests that there is very little or no intraspecific genetic diversity among the seed proteins of bambara groundnut accessions studied. There was wide variation in the content of the five antinutritional compounds among the thirty bambara groundnut accessions. The mean values for condensed tannin content ranged between 0.20 - 6.20 mg/g. Free phosphate recorded an overall mean of 1.71 mg/g while a range of 1.35 - 4.93 mg/g was observed by phytic acid phosphate (PAP). The polyphenol content had an overall mean of 0.39 mg/g and trypsin inhibitor (TIA) was quite variable among the bambara groundnut accessions ranging from 5.30 - 73.40 TIA/mg. Generally, higher levels of antinutrients were observed in this study compared to the other studies. The results obtained in this study led to a conclusion that although variations exits among the accessions studied, further research is required to verify the extent of morphological variations, the efficiency of protein extractions methods evaluated and the effects of these antinutrients in human and animal feeds.

Thesis (MTech (Food Technology))--Cape Peninsula University of Technology, 2015. / The aim of this study was to characterise the physicochemical, rheological, prebiotic and emulsion stabilising properties of four varieties (black-eye, brown-eye, brown and red) of Bambara groundnut (BGN) extracted using the modified wet milling method. A relatively high yield of BGN dietary fibres was obtained with soluble dietary fibres (SDFs) ranging from 15.4 to 17.1% and insoluble dietary fibres (IDFs) ranging from 12.0 to 15.6%. Black-eye and brown-eye dietary fibres showed superiority in terms of swelling capacities, water holding capacities, oil binding capacities, antioxidant properties as well as thermal stabilities than red and brown dietary fibres. In addition, black-eye and brown-eye dietary fibres were characterised by higher lightness (L*), redness (+a*), yellowness (+b*), chroma (C*) and hue. All four SDFs showed acceptable colour differences with ΔE < 8 ranging from 0.81 to 3.08. The hydrolysable polyphenolic (HPP) content of SDFs ranged from 6.89 to 20.86 mg/g GAE and that of IDFs ranged from 10.96 to 14.43 mg/g GAE. All four SDFs differed significantly (p < 0.05) in their HPP content. BGN IDFs were very low in tannins (< 2.2 mg/g).

Diante da necessidade de programas de melhoramento do feijão-caupi para condições de Cerrado, a escolha dos genitores é uma decisão crucial que pode ser auxiliada por ferramentas como análises de divergência genética e análises dialélicas. Sendo assim, o presente trabalho teve como objetivo selecionar genitores de feijão-caupi para programa de melhoramento em condições de Cerrado no Tocantins. Todos os experimentos foram realizados na estação experimental da Universidade Federal do Tocantins e as características avaliadas foram: dias para florescimento, dias para maturação das vagens, número de vagens por planta, número de grãos por vagem, comprimento de vagens, índice de grãos, massa de cem grãos e produtividade de grãos. Com a análise de divergência genética objetivou-se avaliar 24 genótipos de feijão-caupi em condições de Cerrado no Estado do Tocantins. As distâncias entre genótipos foram obtidas através da distância generalizada de Mahalanobis e os genótipos agrupados pelos métodos de Tocher e UPGMA. Foram identificadas diferentes combinações que podem gerar populações promissoras para serem utilizadas em programas de melhoramento visando à produtividade de grãos do feijão-caupi em condições de Cerrado no Tocantins. A produtividade de grãos (24,38%) e o número de grãos por vagem (16,23%) foram as características que mais contribuíram para a dissimilaridade dos genótipos avaliados. No estudo de análise dialélica objetivou-se avaliar a capacidade combinatória de feijão-caupi em condições de Cerrado no Tocantins. Foi utilizado esquema de dialelo parcial 5x3, sendo a análise realizada de acordo com o modelo proposto por Griffing (1956), adaptado a dialelo parcial por Geraldi & Miranda-Filho (1988). As combinações híbridas UFTfc-01 x UFTfc-17, UFTfc-12 x UFTfc-16, UFTfc-10 x UFTfc-05 e UFTfc-10 x UFTfc-16 apresentam potencial para gerar populações segregantes promissoras, visto que apresentaram elevadas estimativas de CEC (acima de 700 kg ha-1) para a produtividade e rendimentos médios superiores a 3.000 kg ha-1. Com as duas metodologias utilizadas observou-se a existência de variabilidade e o potencial de alguns genótipos para serem utilizados como genitores em programas de melhoramento no Sul do Estado do Tocantins. / Given the need for cowpea breeding programs for Cerrado conditions, the choice of the parents is a crucial decision that can be assisted by some tools as genetic divergence analysis and diallel analysis. Therefore, the aim of this study was to select the parents of cowpea for the breeding program under Cerrado conditions in Tocantins. All the experiments were performed at the experimental station of Federal University of Tocantins and the characteristics evaluated were: flowering days, days to pods maturation, number of pods per plant, number of grains per pod, pods length, grains index, mass of a hundred grains and grains yield. With the genetic divergence analysis intended to evaluate 24 genotypes of cowpea under Cerrado conditions in the State of Tocantins. The distances between genotypes were obtained by the generalized distance of Mahalanobis and the genotypes grouped by Tocher and UPGMA methods. Different combinations that can generate promising populations for being used in breeding programs aiming the grains productivity of cowpea under Cerrado conditions in Tocantins were identified. The grains productivity (24,38%) and the number of grains per pod (16,23%) were the characteristics that most contributed for the dissimilarity of the genotypes evaluated. In the study of the diallel analysis aimed to evaluate the combinatorial ability of cowpea genotypes under Cerrado conditions in Tocantins. The partial diallel scheme 5 x 3 was used, with the anlisys performed according to the model proposed by Griffing (1956), adapted for partial diallel by Geraldi & Miranda-Filho (1988). The hybrid combinations UFTfc-01 x UFTfc-17, UFTfc-12 x UFTfc-16, UFTfc-10 x UFTfc-05 and UFTfc-10 x UFTfc-16 present the potential to generate promising segregating populations, as presented high estimates of CEC ( above 700 kg ha-1) for productivity and average incomes greater than 3,000 kg ha-1. With the two methodologies used, observed the existence of variability and the potential of some genotypes to be used as parents in breeding programs in the South of the State of Tocantins.

Diante da necessidade de melhorar a produção de culturas anuais em regiões que enfrentam adversidades hídricas, a utilização dos hidrogéis surgem como condicionadores de água no solo, atuando como reserva de água para a planta, e disponibilizando a mesma em momentos de estresse hídrico, melhorando o desenvolvimento e produtividade das plantas. Desta forma, o estudo foi dividido em três capítulos, no primeiro avaliou-se a eficiência da utilização do hidrogel na retenção e disponibilização de água para o desenvolvimento e produção da cultura do feijão Caupi quando submetido a estresse hídrico. Foram avaliados 4 polímeros e 5 concentrações na cultura do feijão caupi, em casa de vegetação. O segundo e terceiro capítulos foram realizados em campo, avaliando-se 2 polímeros e cinco concentrações para a cultura do feijão caupi e soja safra 2015/2016. Conclui-se que o uso de polímeros mostrou-se, para as condições avaliadas, como potencial alternativo para redução de perdas de produtividade resultantes da ação do estresse hídrico. / Facing of the need to improve the production of annual crops in regions undergo through water adversities, the use of hydrogels appears as water conditioner in the soil, acting as a water reserve for the plant, and making it available in times of water stress, on the first chapter. Thus, the study was divided in three chapters. In chapter one efficiency of hydrogel utilization on the retention and availability of water for the development and production of the Caupi bean culture when submitted to water stress was evaluated, thereby 4 polymers and 5 concentrations in cowpea, under greenhouse conditions were evalvated. The second and third chapters were conducted on the field, evaluating 2 polymers and five concentrations for 2015/2016 cowpea and soybean crops. It is concluded that the use of polymers was shown, for the evaluated conditions as an alternative potential to reduce productivity losses resulting from the action of water stress.

O feijão-caupi, através da simbiose com bactérias do gênero Bradyrhizobium, pode obter N através do processo de fixação biológica do N2 (FBN), que é uma das formas de incrementar a produtividade de leguminosas, evitando-se custos com adubos nitrogenados solúveis. As deficiências nutricionais podem interferir na FBN, pois o fósforo, deficiente na maioria dos solos tropicais, tem efeito marcante sobre a atividade da nitrogenase. Com isto objetivou-se com este trabalho avaliar as doses de fósforo na cultura do feijão caupi em condições de casa de vegetação e em campo de cultivo com e sem o uso de inoculante. Os experimentos foram conduzidos, no ano agrícola 2014/15 na Universidade Federal do Tocantins, com a variedade de feijão caupi BRS Nova Era. Para a avaliação promoveu-se a inoculação de uma estirpe padrão já recomendada para a cultura do feijão caupi. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial (2x6) sendo o primeiro fator (Com e sem inoculação) e o segundo fator seis doses de fósforo (0, 30, 60, 90, 120 e 150 kg ha-1 de P2O5), totalizando 12 tratamentos, com três repetições em casa de vegetação e em blocos casualizados com quatro repetições em campo, safra 2014/15. As características avaliadas em casa de vegetação foram Massa Seca da Parte Aérea, Massa Seca das Raízes, Número de Nódulos, Massa Seca dos Nódulos e Acúmulo de fósforo na parte aérea. As características avaliadas em campo foram Florescimento, Clorofila total, Massa Seca das Raízes, Número de Nódulos, Massa Seca dos Nódulos, Massa Seca da Parte Aérea, peso de cem sementes, produtividade e Acúmulo de fósforo na parte aérea. A aplicação de fósforo juntamente com a inoculação proporcionou o acréscimo na massa seca da raiz da cultivar de feijão-caupi Nova Era. A dose de fósforo com inoculante que propiciou a máxima produção de massa seca parte aérea, massa seca da raiz e acúmulo de fósforo na parte aérea foi de 150 kg ha-1 de P2O5, em casa de vegetação. A adubação fosfatada teve influência positiva nas variáveis: número de nódulos e produtividade de grãos de feijão caupi para a região de Gurupi - TO; para as variáveis de número de nódulos e produtividade do feijão-caupi cv BRS Nova Era, observaram um melhor ajuste da equação linear, o que demonstra potencial de resposta pela cultura à adubação fosfatada. / The cowpea through symbiosis with the bacteria of the genus Bradyrhizobium can obtain N through the process of biological nitrogen fixation (BNF) that is one way to increase legumes productivity avoiding costs with soluble nitrogen fertilizers. Nutritional deficiencies can interfere on the BNF, because most of the tropical soils are deficient in phosphorus and phosphorus has effect on nitrogenase activity. This study aimed to evaluate phosphorus doses in cowpea under greenhouse and field conditions, with and without inoculants. The experiments were conducted during 2014/15 crop year at Federal University of Tocantins with the variety of cowpea BRS Nova Era. The inoculation with a standard strain already recommended for cowpea was done for posterior evaluation. An experimental design of randomized blocks in a 2 x 6 factorial arrangement was used, being the first factor (with and without inoculant) and the second factor six doses of phosphorus (0, 30, 60, 90, 120 e 150 kg ha-1 de P2O5), totalizing 12 treatments with three repetitions in greenhouse and a randomized blocks with four repetitions in the field, crop 2014/15. The characteristics evaluated in greenhouse conditions were: shoot dry mass, root dry mass, nodule number and dry mass and phosphorus accumulation in the shoot. The characteristics evaluated in field conditions were: flowering, total chlorophyll, root dry mass, nodule number, nodule dry mass, shoot dry mass, one hundred seeds weigh, productivity and phosphorus accumulation in the shoot. Inoculation and phosphorus application together increased root dry mass in the cowpea cultivar Nova Era. The dose of phosphorus with inoculant that reached the maximum production of shoot dry mass, root dry mass and phosphorus accumulation in the shoot was 150 kg ha-1 of P2O5, in the greenhouse. Phosphorus fertilization had a positive influence on the variables: nodule number and productivity of cowpea grains for the region of Gurupi-TO; for the variables of nodule number and productivity of cowpea, cv BRS Nova Era, was observed a better adjustment of linear equation that shows potential response of the culture to phosphorus fertilization.

O feijão caupi, através da simbiose com bactérias do gênero Bradyrhizobium, pode obter nitrogênio através do processo de fixação biológica do N2 (FBN). A nodulação e a FBN são influenciadas por fatores edafoclimáticos que podem trazer benefícios ou prejuízos ao processo. A disponibilidade de nutrientes está entre os principais fatores que influenciam a FBN e, dentre os principais nutrientes que influenciam tal processo, cita-se o fósforo.Objetivou-se com esse trabalho, avaliar o rendimento de cultivares de feijão caupi em função das estirpes fixadora de nitrogênio associada ou não a adubação fosfatada, no sul do estado do Tocantins em condições de casa de vegetação e em campo. Os experimentos foram conduzidos em casa de vegetação e a campo, um com a cultivar de feijão-caupi, BRS Nova Era e o outro BRS Sempre Verde, com 4 estirpes de rizóbio mais um tratamento sem bactéria, associadas ou não a 6 doses de fósforo.O delineamento utilizado foi em blocos casualizados, disposto em esquema fatorial 5 x 6. A campo os tratamentos foram dispostos em um delineamento experimental blocos casualizados sob arranjo fatorial 3 x 6, sendo o primeiro fator duas estirpes de rizóbio e um tratamento sem e o segundo fator seis doses de P2O5 com 4 repetições. As doses consistiram de 0, 30, 60, 90, 120 e 150 kg ha-1de P2O5, aplicadas na semeadura. As características avaliadas em casa de vegetação foram: altura de planta, diâmetro de colo, massa seca da parte aérea, massa seca das raízes, número de nódulos, massa seca dos nódulos. As características avaliadas em campo foram: clorofila total, massa seca da parte aérea, peso de cem sementes, produtividade e nitrogênio total na parte aérea. As estirpes estudadas associadas ou não as doses de fósforo proporcionaram acréscimo na altura de planta, diâmetro de colmo, massa seca da parte aérea, massa seca da raiz para as duas cultivares de feijão-caupi estudas BRS Nova Era e BRS Sempre Verde. Para os experimentos de campo a cv. BRS Sempre Verde a estirpe mais indicada é INPA 03-11B; para a cv. BRS Vinagre a estirpe BR 3299 foi mais eficiente. A dose de fósforo que condicionou a máxima eficiência agronômica variou de 70 a 110 kg ha-1. / Cowpea beans, through symbiosis with bacteria of the genus Bradyrhizobium, can obtain nitrogen through the biological fixation process of N2 (BNF). Nodulation and BNF are influenced by edaphoclimatic factors that can bring benefits or losses to the process. The availability of nutrients is among the main factors influencing BNF and, among the main nutrients that influence this process, phosphorus is cited. The objective of this work was to evaluate the yield of cowpea cultivars as a function of nitrogen fixing strains associated or not with phosphate fertilization, in the southern state of Tocantins under greenhouse conditions and in the field. The experiments were conducted in a greenhouse and in the field, one with the cowpea bean cultivar BRS Nova Era and the other BRS Sempre Verde with four strains of rhizobium plus a treatment without bacteria, associated or not To 6 doses of phosphorus. The experimental design was a randomized block design, arranged in a factorial scheme 5 x 6. In the field treatments were arranged in a randomized block design under a factorial arrangement 3 x 6, the first factor being two strains of rhizobia and A treatment without and the second factor six doses of P2O5 with 4 replicates. The doses consisted of 0, 30, 60, 90, 120 and 150 kg ha-1 of P2O5, applied at sowing. The characteristics evaluated in greenhouse were plant height, diameter of lap, dry mass of shoot, dry mass of roots, number of nodules, dry mass of nodules. The characteristics evaluated in the field were: total chlorophyll, shoot dry mass, weight of one hundred seeds, productivity and total nitrogen in shoot. The strains studied, whether or not associated with phosphorus doses, increased plant height, stem diameter, dry shoot mass, root dry mass for the two bean cultivars BRS Nova Era and BRS Sempre Verde. For the field-to-cv. BRS sempre verde the most indicated strain is INPA 03-11B; For cv. BRS Vinagre strain BR 3299 was more efficient. The dose of phosphorus that conditioned the maximum agronomic efficiency ranged from 70 to 110 kg ha-1.