Orientador: Adriana Zerlotti Mercadante / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-21T07:45:45Z (GMT). No. of bitstreams: 1 Xavier_AnaAugustaOdorissi_D.pdf: 4199257 bytes, checksum: b4a79d65bfa255c2e573658b211002e9 (MD5) Previous issue date: 2012 / Resumo: Apesar dos carotenoides estarem amplamente distribuídos nos alimentos de origem vegetal, a sua adição a produtos alimentícios de elevado consumo se torna cada vez mais frequente, visto as inúmeras evidências sobre os benefícios destes pigmentos para a saúde humana. Assim, esse trabalho teve por objetivo o desenvolvimento e a validação de métodos para extração e quantificação de carotenoides em iogurte desnatado e em queijo Prato, ambos adicionados de corante contendo luteína, a fim de verificar a estabilidade deste carotenoide durante o armazenamento refrigerado destes produtos na ausência e na presença de luz (1000 lux). Para o queijo Prato, também foi verificado o efeito do tipo de embalagem na estabilidade da luteína, empregando embalagens plásticas transparentes e pigmentadas de cor vermelha. Além disso, a bioacessibilidade in vitro da luteína adicionada ao leite e ao iogurte desnatado, semidesnatado e integral foi avaliada para estimar a fração de luteína adicionada que pode ser absorvida pelo organismo humano, após o consumo destes produtos. O método desenvolvido e validado para determinação da luteína em iogurte incluiu a extração dos carotenoides com tetraidrofurano em vortex, seguida de centrifugação, partição dos extratos para éter etílico/éter de petróleo (2:1) e quantificação espectrofotométrica dos carotenoides em etanol. O método apresentou linearidade na faixa estudada (1,41¿13,42 µg/g, coeficiente de determinação 0,9938),limites de detecção e quantificação de 0,42 µg/g e 1,28 µg/g, respectivamente, baixo desvio padrão relativo (3,4 %) e recuperação variando entre 95 e 103 %. Este método foi empregado para avaliar a estabilidade de luteína adicionada em iogurte desnatado, onde foi verificado que, na concentração adicionada (aproximadamente 10 µg luteína/g iogurte), o carotenoide manteve-se estável durante os 35 dias de armazenamento refrigerado, independentemente das condições de iluminação. O método validado para iogurte não foi eficiente para a extração de carotenoides de queijo Prato, e por isso, um novo método foi desenvolvido e validado. A extração dos carotenoides do queijo Prato foi realizada em almofariz, utilizando acetato de etila como solvente extrator e hyflosupercel como adjuvante de extração. O novo método desenvolvido para queijo Prato demonstrou linearidade na faixa de 0,05 a 1,15 mg/g, com coeficiente de determinação de 0,9984. Os limites de detecção e quantificação foram de 0,02 e 0,06 mg/g, respectivamente, o desvio padrão relativo de 2,85 % e os valores de recuperação variaram entre 93 e 98 %. Na aplicação deste método para monitorar a estabilidade de luteína durante o armazenamento do queijo Prato, constatou-se que não houve alteração dos níveis deste carotenoide durante 56 dias de armazenamento refrigerado. Os queijos armazenados na presença ou ausência de luz, embalados em embalagem transparente ou pigmentada, mantiveram seus níveis de luteína praticamente constantes e não apresentaram diferenças entre si. A análise da bioacessibilidade in vitro da luteína adicionada em leite e iogurte demonstrou que as amostras de leite semidesnatado e integral, assim como o iogurte integral, apresentaram índices de bioacessibilidade entre 19 e 21 %, semelhantes aos já relatados na literatura para alimentos naturais e formulados. Além disso, durante a digestão in vitro foi constatada a hidrólise de cerca de 85 % dos ésteres de luteína presentes na formulação do corante luteína, provavelmente devido à ação da lipase pancreática. Os resultados deste estudo mostram que os métodos desenvolvidos produziram resultados confiáveis na análise de luteína adicionada em iogurte desnatado e queijo Prato, e que os níveis de luteína se mantiveram constantes durante o armazenamento dos produtos, nas condições estudadas. Além disso, do ponto de vista de bioacessibilidade, entre o leite e iogurte adicionados de luteína, apenas aqueles com maiores teores de gordura (leite semidesnatado e integral e iogurte integral) mostraram-se bons veículos para adição de luteína.22:00 1/11/2012 / Abstract: Although carotenoids are widely found in foods from vegetable origin, their addition to food products of high consumption becomes increasingly common due to the abundant evidence about the benefits of these pigments to the human health. Thus, the aim of this work was to develop and validate methods for the extraction and quantification of carotenoids from skimmed yogurt and "Prato¿ cheese added lutein colorant in order to verify the stability of this carotenoid during refrigerated storage in the dark and in illuminated environment (1000 lux). The effect of packaging on the stability of lutein was also determined in the Prato cheese by using transparent and red pigmented plastic packaging. In addition, the in vitro bioaccessibility of lutein added to skimmed, semi-skimmed and whole milk and yogurt was evaluated to estimate the fraction of the added lutein that can be absorbed by the human body after the intake of these products. The method developed and validated for the determination of lutein in yogurt comprised the extraction of carotenoids with tetrahydrofuran by vortexing, followed by centrifugation, partition of the extract to diethyl ether/petroleum ether (2:1) and spectrophotometric quantification of the carotenoids in ethanol. The method was linear in the tested range (1.41 to 13.42 µg/g, coefficient of determination 0.9938), the limits of detection and quantification were 0.42 µg/g and 1.28 µg/g,respectively, the relative standard deviation (3.4 %) was low and the recovery ranged from 95 to 103 %. This method was employed to evaluate the stability of the yogurt with lutein colorant addition, and at the used concentration (ca. 10 mg lutein/g yogurt), the carotenoid was stable during the 35 days of refrigerated storage, regardless the illumination conditions. The method validated for yogurt was not efficient to extract the carotenoids from Prato cheese, and therefore a new method was developed and validated. The carotenoid extraction from Prato cheese was carried out in a mortar with ethyl acetate and hyflosupercel, as an extraction enhancer. The new method developed for Prato cheese was linear in the range from 0.05 to 1.15 mg/g, with a coefficient of determination of 0.9984. The limits of detection and quantification were 0.02 and 0.06 mg/g, respectively, the relative standard deviation was 2.85 % and recovery values ranged between 93 and 98 %.By applying this method to determine the stability of lutein during the storage of Prato cheese, no modifications were observed in the levels of carotenoids during the 56 days of storage. The lutein contents remained constant and no differences were observed regardless the illumination conditions or package type. The analysis of the in vitro bioaccessibility of lutein from milk and yogurt with addition of lutein colorant showed that semi-skimmed (21 %) and whole milk (19 %), and whole yogurt (21 %) had bioaccessibility values similar to those reported in the literature for natural and formulated foods. Furthermore, about 85% of the esters present in the lutein colorant were hydrolized during the in vitro digestion, probably due to the action of the pancreatic lipase. The results showed that the developed methods produce reliable results for the analysis of lutein added to skimmed yogurt and Prato cheese, and that lutein levels remained constant in these products during the storage, under the studied conditions. Moreover, regarding bioacessibility, only the milk and yogurt with lutein colorant samples with high fat content (whole and skimmed milk and whole yogurt) showed to be good vehicles for the addition of lutein / Doutorado / Doutora em Ciência de Alimentos

Microorganisms have traditionally been selected for fermentation of milk on their ability to grow in milk. However nowadays the trend is towards including probiotic bacteria, i.e. those bacteria that have been suggested to provide additional health benefits to the consumer. Probiotic microorganisms have beneficial effects when ingested such as lowered incidence of colon cancer, the suppression of putrefactive and pathogenic bacteria by competitive exclusion and the production of organic acids, diacetyl and bacteriocins and a hypocholesterolemic effect, to mention but a few. To be more effective, the proposed microorganisms must be of human origin. Lactobacillus acidophilus and Bifidobacterium bifidum, which are two of the most successful probiotic bacteria used commercially, are normal inhabitants of the intestine of many animals including humans. They must however maintain their viability and activity in the bio-product in which they are formulated to be available at the right level at the time of consumption. A number of factors affect their viability, including low pH, the type of culture used and availability of essential amino acids. The choice of ingredients in the mix is crucial since it affects a number of mix properties such as pH and availability of nutrients. The demand for low fat products is a very strong driving force on the market place. However, reducing the fat content of such complex products as frozen dairy desserts is very difficult, since fat forms an integral part of the product. Removing fat poses a number of challenges as to which other ingredients to add in its place. Microparticulated whey protein concentrates have been formulated by food technologists to mimic the functionalities of fat especially as far as creaminess is concerned. Little information is available on fat replacement in frozen yoghurt, in terms of both its effects on the survival of probiotic bacteria and on some important quality parameters. Likewise, little research, if any, has been done on the perception of strawberry flavour in fat-free ice-creams and similar products. The purpose of the present study was thus to provide information on fat replacement by a microparticulated whey protein concentrate (Simplesse® 500) in frozen yoghurts and its influence on the survival of 1. acidophilus and B. bifidum. Several quality parameters of the frozen yoghurts were also evaluated. Frozen yoghurts were prepared by inoculating the mix with an ABT (acidophilus, bifidum and thermophilus) culture. Four mixes were formulated to contain 10% (m/m) milk fat, 5% (m/m) milk fat, 5% (m/m) of the microparticulated whey protein concentrate (WPC) and 3.4% (m/m) of the microparticulated WPC respectively. Decreasing milk fat from 10% to 5%, together with the addition of more milk solids-not-fat, did not lead to a significant increase (p > 0.05) in the acidity of the mix. Likewise the buffering capacity in both alkaline and acidic conditions were not significantly different (p > 0.05). Substituting fat with Simplesse® 500 at 3.4% levelled to a significant decrease in the pH of the mix (p < 0.05), which was due to the presence of more weak acids. The addition of the microparticulated WPC also led to an increase in the buffering capacity of the mix, which was due to an increase in weak acids and other buffer systems present in milk such as the citrate and phosphate systems. The addition of more Simplesse® 500 did not result in a significantly higher acidity (p > 0.05) since whey proteins are only weak acids. The viability of all three the bacteria types present in the ABT culture did not seem to be related to the presence or absence of the microparticulated WPC. The numbers of Streptococcus salivarius subsp. thermophilus, 1. acidophilus and of B. bifidum did not differ significantly (p > 0.05) between the four yoghurt mixes after incubation, ageing, whipping and freezing and three weeks of storage. However, only S. salivarius subsp. thermophilus and 1. acidophilus increased in numbers during fermentation in all the yoghurt mixes and only S. salivarius subsp. thermophilus increased significantly (p < 0.05) as a result of ageing in all the yoghurt mixes. Although the addition of the microparticulated WPC led to an increase in the acidity of the yoghurt mixes it also led to an increase in the buffer capacity, which thus helped to maintain the numbers of S. salivarius subsp. thermophilus and 1. acidophilus at the same level as in the mixes containing milk fat. While S. salivarius subsp. thermophilus and 1. acidophilus grew to numbers greater than 107 cfu/g, B. bifidum did not grow at all in any of the yoghurt mixes and the results suggest that they might not be available at the right level to have any therapeutic benefits to the consumers. Decreasing fat content led to an obvious increase in coarseness of frozen yoghurts (p < 0.05). The increasing perceived coarseness could be related to the amount of ice nuclei formed during whipping and freezing. The addition of the microparticulated whey protein concentrate could have resulted in a decrease in the freezing point, low enough to lead to a decrease in the amount of ice nuclei formed as a result of whipping and freezing. Therefore, the amount of unfrozen water available to freeze during hardening increased, thereby leading to an increase in the size of ice crystals. Fat is also known to decrease the size of ice crystals and the presence of more fat could also have led to an increased perception of smoothness. Increasing fat content led to a decrease in the perception of strawberry flavour and an increased perception of an aftertaste (p < 0.05). Fat is known to bind lipophilic compounds as well as decrease the melting rate and mass transfer, both of which will decrease the release of flavour compounds. / Dissertation (MSc (Food Science))--University of Pretoria, 2006. / Food Science / unrestricted

Os objetivos desta pesquisa foram verificar a proporção entre Streptococcus salivarius subsp. thermophilus e Lactobacillus delbrueckii subsp. bulgaricus em iogurte natural, comercial e os efeitos do armazenamento (dentro do prazo de validade) sobre a proporção das bactérias lácticas componentes do produto, o pH, a acidez e a presença de microrganismos psicrófilos. As amostras de iogurte foram adquiridas por trinta dias consecutivos, num total de 30 amostras, de um mesmo lote, e submetidas às análises de pH, acidez titulável e contagem, em meio TPPY – Eriocromo-ágar, de Lactobacillus sp. e Streptococcus sp. (em UFC/mL). Os resultados mostraram que, durante o armazenamento, ocorreram variações nas características físico-químicas do iogurte, como pH que variou de 4,36, inicialmente, para 4,18, e acidez titulável que variou de 0,73% (em ácido láctico) para 1,17%. Streptococcus salivarius subsp. thermophilus e Lactobacillus delbrueckii subsp. bulgaricus foram encontrados na proporção de 2:1, respectivamente, diminuindo para 1:1 devido, provavelmente, às variações de pH e acidez titulável durante o armazenamento, por trinta dias, que corresponde ao prazo de validade. / The objectives of this research were to verify the proportion of Streptococcus salivarius subsp. thermophilus to Lactobacillus delbrueckii subsp. bulgaricus in commercial natural yogurt and evaluate the effect of the period of storage in some characteristics of the product, such as proportion of lactic bacteria, pH, acidity and presence of psychrophiles microorganisms. Yogurts from the same lot were analysed during a period of 30 consecutive days, within the best before date. One sample a day was analysed for pH, acidity and bacterial count (c.f.u./ml) of Streptococcus sp. and Lactobacillus sp. in broth TPPY-Eriochrome-agar. Results indicated that significant changes on physical-chemical characteristics of the yogurt occured during the storage of the product. The pH decreased from 4.36 to 4.18 and the acidity increased from 0.73% (lactic acid) to 1.17% during the period of storage. The proportion of Streptococcus salivarius subsp. thermophilus to Lactobacillus delbrueckii subsp. bulgaricus during the period of storage varied from 2:1 to 1:1, respectively, probably because of the variations of pH and acidity during these 30 days.

El presente trabajo de investigación busca validar la introducción de un nuevo yogurt Premium al mercado peruano, utilizando una base de contenido diferente al de la competencia y la utilización de un insumo poco conocido en Lima, el Cushuro, el cual es altamente nutritivo y no es muy utilizado de forma comercial. Hemos validado las diferentes posturas que teníamos relacionadas al yogurt, se llevó a cabo la elaboración del producto final y según las ventas obtenidas llegamos a proyectar que a partir del segundo año la empresa percibiría utilidad neta positiva, esto debido a la introducción de Cusure al Sector Retail. La propuesta de valor de la empresa es ofrecer al público un producto saludable, con insumos de calidad y nutritivos que puede ser consumido como complemento en el desayuno, un snack de media mañana o ser el postre del almuerzo el cual lo puedes encontrar en Tiendas Especializadas, Canal Online (Venta directa) y Ferias. / This research paper seeks to validate the introduction of a new Premium yogurt to the Peruvian market, using a different content base from the competition and the use of a little-known input in Lima, the Cushuro, which is highly nutritious and is not widely used commercially. We have validated the different positions we had related to yogurt, the elaboration of the final product was carried out and according to the sales obtained we came to project that from the second year the company would perceive positive net profit, this due to the introduction of Cusure to the Sector Retail The value proposition of the company is to offer the public a healthy product, with quality and nutritious inputs that can be consumed as a complement at breakfast, a mid-morning snack or be the dessert of lunch which you can find in Specialty Stores , Online Channel (Direct sale) and Fairs. / Trabajo de investigación

Objetivo: Estimar el contenido de polifenoles y la capacidad antioxidante y del café arábiga (Coffea arabica) orgánico y convencional en el proceso de elaboración de yogur aromatizado con café. Metodología: Fue un estudio de tipo observacional comparativo, transversal. El análisis y preparación de todas las muestras, pasado de café, confitura tipo jalea de café y yogur aromatizado con café, se realizaron en los laboratorios de la Universidad Peruana de Ciencias Aplicadas. La primera fase para la elaboración del producto fue el filtrado del café en donde se utilizaron dos tipos: café arábiga orgánico y café arábiga convencional; la siguiente fase fue la preparación de la confitura tipo jalea de café de ambos tipos, luego la preparación del yogur natural elaborado con cepas probióticas (S. salivarius subsp thermophilus, L. delbrueckii subsp bulgaricus, L. acidophilus, Bifidobacterium spp) y la última fase fue la mezcla de la confitura tipo jalea de café con el yogur natural en concentraciones de 15%, 19% y 23%. Se practicaron análisis para determinar las características fisicoquímicas del producto final: pH, acidez(%), humedad(%), capacidad de retención de agua (%WHC) y sinéresis (%STS). Resultados: El contenido de polifenoles y la capacidad antioxidante es directamente proporcional con la concentración de confitura de café que posee el yogur aromatizado con café. Conclusiones: El café pasado, la confitura tipo jalea de café y el yogur aromatizado con café en su versión convencional presentó mayor contenido de polifenoles y capacidad antioxidante que la versión orgánica. El yogur aromatizado con café orgánico al 23% fue el preferido por los consumidores en la evaluación sensorial. / Tesis

El presente trabajo tiene como objetivo el diseño de un proyecto de optimización del proceso productivo e incremento significativo de la calidad del producto de la planta elaboradora de yogur LAIVE S.A. El mencionado proyecto constituye uno de los puntos claves del planeamiento estratégico de la compañía definido por la alta dirección. La meta es duplicar las ventas entre los años 2006 y 2010. Para ello el producto debe ser de calidad, cumpliendo los requisitos del cliente, homogéneo y a un costo que permita que el negocio sea sostenible en el tiempo. El crecimiento acelerado de la producción de yogur acompañado del inevitable aumento de maquinarias, con presupuestos limitados y sumados a los cambios en los requisitos del consumidor final en los últimos años, han ido en desmedro del rendimiento y han generado sobre costos. El análisis realizado en el año 2007 de los problemas y sus causas ha presentado diversas posibilidades de mejora. Desde el punto de vista de calidad, cambiando parte del proceso y reordenando el layout de planta, se pueden obtener mejoras rápidas y de este modo cumplir con los requisitos del cliente. Estas mejoras de proceso y reordenamiento del layout mejorarán a su vez los rendimientos del producto generando rentabilidad y permitiendo sostenibilidad del negocio en el tiempo. Se pretende regresar a los rendimientos del año 2005 recuperando de esta forma entre 37.2 y 46.5 toneladas de producto al mes. Este estudio concluye que, para revertir la reducción de productividad observada, es necesario intervenir con el rediseño y puesta en práctica de un modelo de optimización del proceso productivo para la elaboración del yogur LAIVE. / Tesis

This diploma thesis deals with the determination of aroma compounds in fruit yoghurts. These compounds are crucial for flavour of these dairy products. Two types of fruit yoghurts were analyzed, creamy (8 % of fat) and light (less than 0,1 % of fat), with various fruit ingredients, produced in dairy Mlékárna Valašské Meziříčí, spol. s r.o. Analysis was carried out by gas chromatography with extraction of analytes by SPME - solid phase microextraction. Six groups of different aromatic compounds such as ketones, aldehydes, organic acids and their salts, alcohols and sulfides in different amounts were determined. These compounds have mostly microbiological origin (Streptococcus salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus are dairy yoghurt cultures usually used for yoghurt production), less amounts are present in cow's milk or in dairy additives. Yoghurts were simultaneously sensory evaluated using ranking test, scale test and pair test for determination of acceptability and flavour. The assessors were students and staffs faculty of chemistry VUT.

This diploma thesis deals with the questions of determination of aroma active compounds present in yoghurts. These compounds are crucial for flavour of these fermented dairy products. Four types of yoghurts were analyzed, creamy (10 % of fat), medium-fat and medium-fat Bio (3 % of fat) and light (0,1 % of fat), produced by Mlékárna Valašské Meziříčí, spol. s r.o. Analysis was carried out by gas chromatography with extraction of analytes by SPME - solid phase microextraction. Five groups of different aroma active compounds such as ketones, aldehydes, organic acids and their esters and alcohols in different amounts were determined. These compounds are mostly metabolic products of Streptococcus salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, dairy yoghurt cultures usually used for yoghurt production. Less amounts are present in cow's milk or in dairy additives. Yoghurts were simultaneously sensorially evaluated using ranking test and scale test for determination of acceptability and flavour. The assessors were students and staffs of faculty of chemistry BUT.

Ante la necesidad que nuestro equipo de trabajo capto sobre los consumidores de yogurt. Presentamos una alternativa diferente en la cual podamos tener un producto innovador en el mercado. La idea de negocio se planteó estableciendo un nicho de mercado en la cual nos dirigiremos a personas que consumen productos saludables. Esta idea de negocio va permitir captar a un público distinto al de otras grandes marcas. SoyFit está compuesto con productos naturales y con proveedores locales. Asimismo, cada ingrediente está seleccionado de forma que ofrece al público un producto de calidad. Además, el producto es realizado de manera artesanal y distribuido a bioferias, consumidores locales y gimnasios. En las próximas páginas del presente trabajo se detallara como fue el proceso de realización de nuestra idea de negocio. En cada página se presentara las validaciones, modelo negocio, pronósticos, fuentes, etc. Con el objetivo de que nuestro producto sea rentable a largo plazo. En síntesis, al finalizar las validaciones pudimos determinar que nuestro producto es viable y nos generara en los siguientes años un retorno económico junto con utilidades. Con ello se busca seguir creciendo nuestra idea de negocio y quizás en un futuro ofrecerlo en un mercado extranjero. / Given the need for our team to capture yogurt consumers. We present a different alternative in which we can have an innovative product in the market. The business idea was raised by establishing a market niche in which we will address people who consume healthy products. This business idea will allow to attract a different audience from other major brands. SoyFit is made up of natural products and local suppliers. Likewise, each ingredient is selected in a way that offers the public a quality product. In addition, the product is made by hand and distributed to bioferias, local consumers and gyms. In the next pages of this work we will detail how the process of realization of our business idea was. Validations, business model, forecasts, sources, etc. will be presented on each page. With the aim of making our product profitable in the long term. In summary, at the end of the validations we could determine that our product it is viable and will generate in the following years an economic return along with utilities. This seeks to continue growing our business idea and perhaps in the future offer it in a foreign market. / Trabajo de investigación

El presente proyecto es un producto natural para personas con un estilo de vida saludable e intolerantes a la lactosa, este cuenta con viabilidad en su desarrollo, debido a la investigación realizada, en el que se segmenta a la población de Lima Metropolitana de los niveles socioeconómicos A, B y C, donde se valida un alto interés por el producto y la falta de una bebida 100% natural deslactosada y con un sabor agradable en el mercado. Asimismo, se reconoció que las personas cuentan con poca disponibilidad de tiempo para preparar sus alimentos, debido a las actividades que realizan en el día, por lo que, recurren mayormente a productos prácticos en el momento de su alimentación. Además, existe una creciente demanda de alimentos saludables y naturales, debido al incremento de personas que adoptan un estilo de vida saludable. Por último, las personas intolerantes a la lactosa cuentan con pocas alternativas de productos alimenticios y de sabor agradables en el mercado. Para que este proyecto se lleve a cabo, se realizó un estudio de mercado que involucró, en primer lugar, al consumidor, proveedores y distribuidores. También se analizó el proceso de cadena de valor para el negocio, para lo cual se tomó en cuenta la aplicación de estrategias operacionales, de responsabilidad social, de marketing y de desarrollo financiero, que tienen por finalidad mantener la rentabilidad y viabilidad del proyecto a largo plazo en el mercado. De este modo, la inversión para ejecutar este proyecto sería de S/ 9,881.54, con el que se obtendrá una utilidad neta de S/ -18,869.00 en el primer año, -S/ 62,426.00 en el segundo año y -S/80,670.00 en el tercer año. / The present project is a natural product for people with a healthy lifestyle and lactose intolerant, it has a feasibility in its development, due to the research carried out, in which the population of Metropolitan Lima is segmented from socioeconomic levels A, B and C, which validates a high interest in the product and the lack of a 100% natural drink lactose and with a pleasant taste in the market. Likewise, it was recognized that people have little availability of time to prepare their food, due to the activities they carry out during the day, so they use mostly practical products at the time of feeding. In addition, there is a growing demand for healthy and natural foods, due to the increase in people adopting a healthy lifestyle. Finally, lactose intolerant people have few alternatives for food and taste products that are pleasant in the market. For this project to be carried out, a market study was carried out that involved, first, the consumer, suppliers and distributors. The value chain process for the business was also analyzed, for which the application of operational, social responsibility, marketing and financial development strategies was taken into account, which aim to maintain the profitability and viability of the project over the long term. term in the market. In this way, the investment to execute this project would be S /S/ 9,881.54, with which a net profit of -S/ 18,869.00 will be obtained in the first year, -S/ 62,426.00in the second year and -S/80,670.00 in the third year. / Trabajo de investigación