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温度がチェリモヤの生殖器官に及ぼす影響 / Effects of Temperature on Cherimoya Reproductive Organs松田, 大志 23 March 2015 (has links)
Kyoto University (京都大学) / 0048 / 新制・課程博士 / 博士(農学) / 甲第19043号 / 農博第2121号 / 新制||農||1032 / 31994 / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 縄田 栄治, 教授 田中 千尋, 教授 北島 宣 / 学位規則第4条第1項該当
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Anatomical Study of the Greater Palatine Artery: Clinical Implications for Palatal Graft ProceduresCunningham, Nina Marie Karin 28 February 2016 (has links)
Introduction: The palate is a well-established donor site for obtaining graft tissue in periodontal plastic surgery procedures. However, proximity to the adjacent teeth on the lateral aspect and the greater palatine neurovascular bundle (GPB) on the medial aspect limit the amount of graft tissue that can be obtained from the palate. Previous studies have been concerned with the location of the greater palatine foramen as well as the greater palatine artery (GPA) and have established guidelines on how to estimate the distance between the teeth and the GPB. Traditionally, clinicians follow these guidelines and choose to avoid removing graft tissue in the area close to the GPB out of fear of possible complications such as hemorrhaging and paresthesias. Objectives: The purpose of the present investigation is to locate the position of the greater palatal artery (GPA) in relation to surrounding anatomical landmarks and determine if the tissue thickness covering the GPA is sufficient to permit gingival grafts to be obtained in the area close to the GPB. Materials and methods: Cadaver dissections were performed on a total of ten (n=10) cadaver hemifaces of which 7 were partially and 3 were completely edentulous. From the greater palatine foramen to the incisive foramen, the palatal tissues of the cadavers were dissected into vertical slices of 3 mm in width perpendicular to the median palatine raphe using a double bladed scalpel. On each tissue slice, the distance from the epithelial surface to the superior border of the vessel, the diameter of the vessel, the distance from the inferiorborder of the vessel to the palatal bone, the distance from median palatine raphe to the GPA and the distance from teeth or midline of the alveolar crest to the GPA were measured using both a periodontal probe and a digital caliper. The measurements were correlated to each other, the angle of the palatal vault, an estimate of the palatal depth and the head length of the cadavers. Results: The mean thickness of the tissue above the GPA was 4.30 ± 1.61 mm with a range of 1.92 – 8.72 mm. The tissue thickness decreased consistently from the 3rd molar to the canine area with the thickest mean tissue being in the 2nd molar region with 6.25 ± 1.09 mm and shallowest mean tissue thickness in the region of the lateral incisor with 2.92 ± 0.46 mm. The mean distance of the GPA from the median palatine raphe is 10.34 ± 3.41mm ranging from 13.77 ± 1.67 mm to 6.02 ± 0.83 mm with the greatest distance being from the 3rd molar region and smallest distance being from the lateral incisor area. No statistically significant correlations were found between the angel of the palatal vault, the estimate of the palatal depth and the head length. A significant correlation (R2=0.92) was found between the total palatal tissue thickness and tissue thickness above the GPA. Discussion: There was adequate gingival tissue above the GPA to harvest tissue for free gingival grafts of 1 - 1.5 mm in thickness in the entire palate. Donor tissue for 1.5 mm thick connective tissue grafts with a 1.5 mm epithelial flap could be obtained opposing the 1st molar and posterior to it staying above the GPA. Donor site for palatal grafts can be extended in a medial and posterior direction.A Formula (Tissue Thickness above the GPA = (Total Thickness of palatal tissue - 0.967) x 0.9) has been derived, which accurately locates the GPA based on the thickness of the palatal tissue. Unique to this study were measurements from the median palatine raphe, which will provide the clinician with a new landmark to more reliably locate the GPA at various locations on the palate. Conclusion: This descriptive pilot study on human cadavers provides a formula to locate the GPA within the palate using the total palatal tissue thickness and suggests that graft tissue can be harvested from the tissue above the GPA in the entire palate for FGGs and opposing to the 1st molar and posterior to it for CTGs not exceeding 3 mm in depth.
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Effects of Temperature on Cherimoya Reproductive Organs / 温度がチェリモヤの生殖器官に及ぼす影響Matsuda, Hiroshi 23 March 2015 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19043号 / 農博第2121号 / 新制||農||1032(附属図書館) / 学位論文||H27||N4925(農学部図書室) / 31994 / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 縄田 栄治, 教授 田中 千尋, 教授 北島 宣 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM
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Estudo anatômico do tronco encefálico por imagens de ressonância magnética de 3 Teslas e correlação com cortes histológicos / Anatomical study of brainstem magnetic resonance images of 3 Teslas and correlation with histological sectionsFreitas, Lincoln da Silva 21 March 2016 (has links)
O Tronco encefálico é uma estrutura singular do sistema nervoso central, pois nele passam tratos sensoriais ascendentes da medula espinal, tratos sensoriais da cabeça e do pescoço, os tratos descendentes motores originados no prosencéfalo, as vias ligadas a centros de movimento dos olhos, contemos núcleos dos nervos cranianos, e também está envolvido na regulação do nível de consciência através de projeções ao prosencéfalo oriundas da formação reticular. Tudo isto compactado em um espaço muito exíguo o que faz deste um local particularmente sensível às alterações patológicas, mesmo que pequenas, que acabam cursando com uma riqueza de sinais neurológicos devido a presença muito próxima das estruturas já citadas. Compreender a anatomia interna do tronco encefálico é essencial para o diagnóstico neurológico e a prática da medicina clínica. Por tudo que foi exposto, o tronco encefálico é um terreno fértil para o estudo através do diagnóstico por imagem, principalmente quando realizado por novas tecnologias, como exames em aparelho de ressonância magnética de alto campo (3 teslas). No entanto, pouco se sabe sobre as correlações existentes entre a microscopia e as imagens de ressonância magnética do tronco encefálico. Sendo assim, o objetivo deste estudo foi analisar e correlacionar as diversas estruturas encontradas no tronco encefálico, visualizadas em peças microscópicas de encéfalos humanos post mortem, com as imagens de ressonância magnética dos mesmos, antes da dissecção, mapeandoas e discernindo-as, contribuindo assim para diagnósticos mais precisos e topográficos das patologias que acometem o tronco encefálico, justificando o presente estudo. O estudo foi de caráter observacional exploratório e descritivo, adotando as seguintes técnicas para coleta da informações: os encéfalos humanos (n=3) foram submersos em recipiente contendo água e então lacrados de forma que o ar ambiente não entrasse no recipiente. As imagens de RM foram adquiridas em sequência gradiente echo (FFE) 2D em equipamento de campo 3T (PHILIPS ACHIEVA), com bobina de 8 canais de encéfalo. Foi utilizado TE=9,0 ms, TR=1000 ms e o ângulo de flip 90°; número de médias igual 10 e BW por pixel igual a 72 Hz/pixel. O Fator EPI utilizado foi igual a um e a resolução espacial de 0,219x0,219x2,0 mm3 com FOV de 210x210x90mm3. O tempo total de aquisição foi de 3 horas e 01 minuto e 96 segundos. Imagens histológicas utilizadas no presente estudo são do banco de dados do departamento de patologia da UNICAMP e foram comparadas às imagens obtidas na ressonância magnética. Demonstramos que foi possível a identificação das estruturas visíveis, histologicamente, nas imagens obtidas, com definição e resolução suficiente para a geração de um atlas de imagens de ressonância magnética de cortes do tronco encefálico / The Brainstem is a unique structure of the central nervous system, because in it pass ascending sensory tracts of the spinal cord, sensory tracts of head and neck, descending tracts originated in the forebrain, the pathways linked to eye movement centers, contains nuclei of cranial nerves, and is also involved in regulating the level of consciousness through projections to the forebrain that arise from the reticular formation. All these estructures are packed into a very small space which makes the brainstem a particularly sensitive place to pathological changes, that bring up a large amount of neurological signs due to very close packing of the aforementioned structures.Understanding the internal anatomy of the brainstem is essential for the neurological diagnosis and the clinical medicine practice. Thus, the brainstem is fertile ground for the study through diagnostic imaging, especially when performed by new technologies such as high-field (3 tesla) MRI machines. However, little is known about the correlation between the microscopy and magnetic resonance imaging of the brainstem. The aim of this study was to analyze and correlate the various structures found in the brainstem, viewed in microscopic slides of human brains post mortem, with the magnetic resonance imaging thereof, prior to dissection, mapping them and defining them, thus contributing to more accurate diagnoses and surveying of pathologies that affect the brainstem. Human brains (n = 3) were submerged in a container containing water, and then sealed so that the ambient air does not enter the container. MRI images were acquired in gradient echo sequence (FFE) 2D 3T field equipment (PHILIPS ACHIEVA) with coil 8- channel brain. It was used TE = 9.0 ms, TR = 1000 ms and flip angle 90°; number of averages equal to 10 and BW per pixel equal to 72 Hz/pixel. Factor PPE used was equal to one and the spatial resolution of FOV with 0,219x0,219x2,0 mm3 210x210x90mm3. The total acquisition time was 3 hours, 01 minute and 96 seconds. Histological images used in this study are from the pathology department of State University of Campinas (UNICAMP) database and compared to images obtained in MRI. We demonstrated that it was possible to identify histologically visible structures in images acquired with sufficient resolution and definition to generate an magnetic resonance imaging atlas of the brainstem sections
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Estudo anatômico do tronco encefálico por imagens de ressonância magnética de 3 Teslas e correlação com cortes histológicos / Anatomical study of brainstem magnetic resonance images of 3 Teslas and correlation with histological sectionsLincoln da Silva Freitas 21 March 2016 (has links)
O Tronco encefálico é uma estrutura singular do sistema nervoso central, pois nele passam tratos sensoriais ascendentes da medula espinal, tratos sensoriais da cabeça e do pescoço, os tratos descendentes motores originados no prosencéfalo, as vias ligadas a centros de movimento dos olhos, contemos núcleos dos nervos cranianos, e também está envolvido na regulação do nível de consciência através de projeções ao prosencéfalo oriundas da formação reticular. Tudo isto compactado em um espaço muito exíguo o que faz deste um local particularmente sensível às alterações patológicas, mesmo que pequenas, que acabam cursando com uma riqueza de sinais neurológicos devido a presença muito próxima das estruturas já citadas. Compreender a anatomia interna do tronco encefálico é essencial para o diagnóstico neurológico e a prática da medicina clínica. Por tudo que foi exposto, o tronco encefálico é um terreno fértil para o estudo através do diagnóstico por imagem, principalmente quando realizado por novas tecnologias, como exames em aparelho de ressonância magnética de alto campo (3 teslas). No entanto, pouco se sabe sobre as correlações existentes entre a microscopia e as imagens de ressonância magnética do tronco encefálico. Sendo assim, o objetivo deste estudo foi analisar e correlacionar as diversas estruturas encontradas no tronco encefálico, visualizadas em peças microscópicas de encéfalos humanos post mortem, com as imagens de ressonância magnética dos mesmos, antes da dissecção, mapeandoas e discernindo-as, contribuindo assim para diagnósticos mais precisos e topográficos das patologias que acometem o tronco encefálico, justificando o presente estudo. O estudo foi de caráter observacional exploratório e descritivo, adotando as seguintes técnicas para coleta da informações: os encéfalos humanos (n=3) foram submersos em recipiente contendo água e então lacrados de forma que o ar ambiente não entrasse no recipiente. As imagens de RM foram adquiridas em sequência gradiente echo (FFE) 2D em equipamento de campo 3T (PHILIPS ACHIEVA), com bobina de 8 canais de encéfalo. Foi utilizado TE=9,0 ms, TR=1000 ms e o ângulo de flip 90°; número de médias igual 10 e BW por pixel igual a 72 Hz/pixel. O Fator EPI utilizado foi igual a um e a resolução espacial de 0,219x0,219x2,0 mm3 com FOV de 210x210x90mm3. O tempo total de aquisição foi de 3 horas e 01 minuto e 96 segundos. Imagens histológicas utilizadas no presente estudo são do banco de dados do departamento de patologia da UNICAMP e foram comparadas às imagens obtidas na ressonância magnética. Demonstramos que foi possível a identificação das estruturas visíveis, histologicamente, nas imagens obtidas, com definição e resolução suficiente para a geração de um atlas de imagens de ressonância magnética de cortes do tronco encefálico / The Brainstem is a unique structure of the central nervous system, because in it pass ascending sensory tracts of the spinal cord, sensory tracts of head and neck, descending tracts originated in the forebrain, the pathways linked to eye movement centers, contains nuclei of cranial nerves, and is also involved in regulating the level of consciousness through projections to the forebrain that arise from the reticular formation. All these estructures are packed into a very small space which makes the brainstem a particularly sensitive place to pathological changes, that bring up a large amount of neurological signs due to very close packing of the aforementioned structures.Understanding the internal anatomy of the brainstem is essential for the neurological diagnosis and the clinical medicine practice. Thus, the brainstem is fertile ground for the study through diagnostic imaging, especially when performed by new technologies such as high-field (3 tesla) MRI machines. However, little is known about the correlation between the microscopy and magnetic resonance imaging of the brainstem. The aim of this study was to analyze and correlate the various structures found in the brainstem, viewed in microscopic slides of human brains post mortem, with the magnetic resonance imaging thereof, prior to dissection, mapping them and defining them, thus contributing to more accurate diagnoses and surveying of pathologies that affect the brainstem. Human brains (n = 3) were submerged in a container containing water, and then sealed so that the ambient air does not enter the container. MRI images were acquired in gradient echo sequence (FFE) 2D 3T field equipment (PHILIPS ACHIEVA) with coil 8- channel brain. It was used TE = 9.0 ms, TR = 1000 ms and flip angle 90°; number of averages equal to 10 and BW per pixel equal to 72 Hz/pixel. Factor PPE used was equal to one and the spatial resolution of FOV with 0,219x0,219x2,0 mm3 210x210x90mm3. The total acquisition time was 3 hours, 01 minute and 96 seconds. Histological images used in this study are from the pathology department of State University of Campinas (UNICAMP) database and compared to images obtained in MRI. We demonstrated that it was possible to identify histologically visible structures in images acquired with sufficient resolution and definition to generate an magnetic resonance imaging atlas of the brainstem sections
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Estudo da anatomia endoscópica do acesso transpterigóideo e da artéria carótida interna por via endonasal / Anatomical study of the endoscopic endonasal transpterygoid approach and the internal carotid arteryFortes, Felipe Sartor Guimarães 17 August 2011 (has links)
INTRODUÇÃO. Durante a última década, os avanços tecnológicos, o maior domínio da anatomia e das técnicas operatórias e de reconstrução da base do crânio por via endonasal propiciaram a expansão da cirurgia endoscópica para além dos limites da sela túrcica. O acesso transpterigóideo é uma etapa comum ao acesso as fossas cranianas média e posterior, e o conhecimento da anatomia endoscópica da artéria carótida interna (ACI) é um ponto fundamental e comum a estes acessos. OBJETIVO. Descrever a anatomia endoscópica relacionada ao acesso transpterigóideo e da ACI utilizando um modelo anatômico em cabeças cadavéricas frescas procurando definir os parâmetros seguintes: reparos anatômicos do acesso endoscópico a região supra e infrapetrosa, os limites para exposição endoscópica da ACI, os reparos anatômicos para localização dos seus segmentos lacerum, petroso e parafaríngeo. MÉTODOS. Foi realizado estudo anatômico em 20 espécimes (10 cabeças) de cadáveres frescos. As cabeças foram previamente preparadas com a injeção intravascular de silicone corado para enaltecer as estruturas vasculares (ACI, artéria maxilar e seus ramos e seio cavernoso). Em todos os casos foi realizado o acesso transpterigóideo às regiões supra e infrapetrosa, assim como a fossa infratemporal, e a dissecção endoscópica da ACI. RESULTADOS. A injeção de silicone corado no sistema arterial e venoso proporcionou modelo anatômico adequado para dissecção e documentação do acesso transpterigóideo assim como dos diferentes segmentos da ACI. A dissecção por etapas proporcionou exposição adequada da ACI do seu segmento cavernoso ao parafaríngeo distal. O acesso transpterigóideo forneceu exposição adequada do segmento lacerum da ACI e região suprapetrosa e seus principais reparos anatômicos foram o nervo vidiano, nervos maxilar e mandibular (V2, V3) e gânglio de Gasser. A exposição caudal da ACI (segmentos petroso e parafaríngeo) e região infrapetrosa requer maxilectomia medial para exposição e remoção de todo o processo pterigóideo, seguida pela ressecção da tuba auditiva e do tecido fibrocartilaginoso do forame lacerum. Os principais reparos anatômicos desta etapa foram a tuba auditiva e V3. CONCLUSÃO. Durante o acesso endoscópico à região suprapetrosa, o nervo vidiano, os ramos maxilar e mandibular do nervo trigêmeo (V2 e V3) e o gânglio de Gasser são reparos anatômicos para localização da artéria carótida interna e fossa craniana média. Durante o acesso endoscópico à região infrapetrosa, o tecido fibrocartilaginoso do forame lacerum, a tuba auditiva e o nervo mandibular (V3) são reparos anatômicos para identificação da artéria carótida interna. O acesso endoscópico endonasal por etapas permite acesso à artéria carótida interna extracraniana desde o segmento cavernoso até seu segmento parafaríngeo inferiormente ao nível do assoalho da cavidade nasal. O reparo anatômico para localização do segmento lacerum e porção horizontal do segmento petroso da ACI é o nervo vidiano; para as porções petrosa horizontal junto ao joelho posterior e canal carotídeo os reparos anatômicos são o nervo mandibular (V3), forame oval, tecido fibrocartilaginoso do forame lacerum e a tuba auditiva; para o segmento parafaríngeo os reparos anatômicos são o tronco posterior do nervo mandibular (V3) e a tuba auditiva / INTRODUCTION. During the last decade, the technological advances, the mastery of the anatomy and operative techniques and skull base reconstruction using endonasal approaches have propelled endoscopic surgery for beyond the limits of sella turcica. The transpterygoid approach is a common step of the endoscopic approach to the middle and posterior cranial fossa and knowledge of the endoscopic anatomy of the internal carotid artery (ICA) is a common and paramount point to these approaches. OBJECTIVE. Describe the endoscopic anatomy related to the transpterygoid approach and ICA using an anatomical model with fresh human cadaveric heads to define: anatomical landmarks related to the endoscopic supra and infrapetrous areas approach, limits for endoscopic exposure of the ICA, anatomical endoscopic landmarks to lacerum, petrous and parapharyngeal ICA segments. METHODS. An anatomical study was performed using 20 specimens (10 heads) of fresh cadaveric heads. The heads were previously prepared with the injection of colored silicone to enhance the vascular structures (ICA, maxillary artery and its branches and cavernous sinus). In all cases we performed the transpterygoid approach to the supra and infrapetrous areas, as well as the infratemporal fossa, and the endoscopic dissection of the ICA. RESULTS. The injection of colored silicone in the arterial and venous system provided an adequate anatomical model for dissection and documentation of the transpterygoid approach as well as the different segments of the ICA. A stepwise dissection provided adequate exposure of the ICA from its cavernous to the distal parapharyngeal segment. The transpterygoid approach provided adequate exposure of the lacerum segment of the ICA and suprapetrous area and the landmarks were the vidian nerve, maxillary and mandibular nerves (V2, V3) and the Gasserian ganglion. Exposure of the caudal ICA (petrous and parapharyngeal) and the infrapetrous area required a medial maxillectomy for exposure and resection of the entire pterygoid process, followed by the Eustachian tube and the fibrocartilaginous tissue of the foramen lacerum. The main anatomical landmarks to this step were V3 and the Eustachian tube. CONCLUSION. During the endoscopic approach to the suprapetrous area, the vidian nerve, the maxillary and mandibular divisions of the trigeminal nerve (V2, V3) and the Gasserian ganglion are the anatomical landmarks to the internal carotid artery and middle cranial fossa. During the endoscopic approach to the infrapetrous area, the fibrocartilaginous tissue of the foramen lacerum, the Eustachian tube and V3 are the landmarks to the internal carotid artery. A stepwise endoscopic endonasal approach provided access to the extracranial internal carotid artery from its cavernous to the parapharyngeal segment down to the level of the nasal fossa floor. The anatomical landmark to the lacerum and horizontal petrous segments of the internal carotid artery is the vidian nerve; to the horizontal petrous segment before the posterior bend and carotid canal, the anatomical landmarks are the mandibular nerve (V3), foramen ovale, fibrocartilaginous tissue of the foramen lacerum and the Eustachian tube; to the parapharyngeal segment, the anatomical landmarks are the posterior trunk of the mandibular nerve (V3) and the Eustachian tube
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Estudo da anatomia endoscópica do acesso transpterigóideo e da artéria carótida interna por via endonasal / Anatomical study of the endoscopic endonasal transpterygoid approach and the internal carotid arteryFelipe Sartor Guimarães Fortes 17 August 2011 (has links)
INTRODUÇÃO. Durante a última década, os avanços tecnológicos, o maior domínio da anatomia e das técnicas operatórias e de reconstrução da base do crânio por via endonasal propiciaram a expansão da cirurgia endoscópica para além dos limites da sela túrcica. O acesso transpterigóideo é uma etapa comum ao acesso as fossas cranianas média e posterior, e o conhecimento da anatomia endoscópica da artéria carótida interna (ACI) é um ponto fundamental e comum a estes acessos. OBJETIVO. Descrever a anatomia endoscópica relacionada ao acesso transpterigóideo e da ACI utilizando um modelo anatômico em cabeças cadavéricas frescas procurando definir os parâmetros seguintes: reparos anatômicos do acesso endoscópico a região supra e infrapetrosa, os limites para exposição endoscópica da ACI, os reparos anatômicos para localização dos seus segmentos lacerum, petroso e parafaríngeo. MÉTODOS. Foi realizado estudo anatômico em 20 espécimes (10 cabeças) de cadáveres frescos. As cabeças foram previamente preparadas com a injeção intravascular de silicone corado para enaltecer as estruturas vasculares (ACI, artéria maxilar e seus ramos e seio cavernoso). Em todos os casos foi realizado o acesso transpterigóideo às regiões supra e infrapetrosa, assim como a fossa infratemporal, e a dissecção endoscópica da ACI. RESULTADOS. A injeção de silicone corado no sistema arterial e venoso proporcionou modelo anatômico adequado para dissecção e documentação do acesso transpterigóideo assim como dos diferentes segmentos da ACI. A dissecção por etapas proporcionou exposição adequada da ACI do seu segmento cavernoso ao parafaríngeo distal. O acesso transpterigóideo forneceu exposição adequada do segmento lacerum da ACI e região suprapetrosa e seus principais reparos anatômicos foram o nervo vidiano, nervos maxilar e mandibular (V2, V3) e gânglio de Gasser. A exposição caudal da ACI (segmentos petroso e parafaríngeo) e região infrapetrosa requer maxilectomia medial para exposição e remoção de todo o processo pterigóideo, seguida pela ressecção da tuba auditiva e do tecido fibrocartilaginoso do forame lacerum. Os principais reparos anatômicos desta etapa foram a tuba auditiva e V3. CONCLUSÃO. Durante o acesso endoscópico à região suprapetrosa, o nervo vidiano, os ramos maxilar e mandibular do nervo trigêmeo (V2 e V3) e o gânglio de Gasser são reparos anatômicos para localização da artéria carótida interna e fossa craniana média. Durante o acesso endoscópico à região infrapetrosa, o tecido fibrocartilaginoso do forame lacerum, a tuba auditiva e o nervo mandibular (V3) são reparos anatômicos para identificação da artéria carótida interna. O acesso endoscópico endonasal por etapas permite acesso à artéria carótida interna extracraniana desde o segmento cavernoso até seu segmento parafaríngeo inferiormente ao nível do assoalho da cavidade nasal. O reparo anatômico para localização do segmento lacerum e porção horizontal do segmento petroso da ACI é o nervo vidiano; para as porções petrosa horizontal junto ao joelho posterior e canal carotídeo os reparos anatômicos são o nervo mandibular (V3), forame oval, tecido fibrocartilaginoso do forame lacerum e a tuba auditiva; para o segmento parafaríngeo os reparos anatômicos são o tronco posterior do nervo mandibular (V3) e a tuba auditiva / INTRODUCTION. During the last decade, the technological advances, the mastery of the anatomy and operative techniques and skull base reconstruction using endonasal approaches have propelled endoscopic surgery for beyond the limits of sella turcica. The transpterygoid approach is a common step of the endoscopic approach to the middle and posterior cranial fossa and knowledge of the endoscopic anatomy of the internal carotid artery (ICA) is a common and paramount point to these approaches. OBJECTIVE. Describe the endoscopic anatomy related to the transpterygoid approach and ICA using an anatomical model with fresh human cadaveric heads to define: anatomical landmarks related to the endoscopic supra and infrapetrous areas approach, limits for endoscopic exposure of the ICA, anatomical endoscopic landmarks to lacerum, petrous and parapharyngeal ICA segments. METHODS. An anatomical study was performed using 20 specimens (10 heads) of fresh cadaveric heads. The heads were previously prepared with the injection of colored silicone to enhance the vascular structures (ICA, maxillary artery and its branches and cavernous sinus). In all cases we performed the transpterygoid approach to the supra and infrapetrous areas, as well as the infratemporal fossa, and the endoscopic dissection of the ICA. RESULTS. The injection of colored silicone in the arterial and venous system provided an adequate anatomical model for dissection and documentation of the transpterygoid approach as well as the different segments of the ICA. A stepwise dissection provided adequate exposure of the ICA from its cavernous to the distal parapharyngeal segment. The transpterygoid approach provided adequate exposure of the lacerum segment of the ICA and suprapetrous area and the landmarks were the vidian nerve, maxillary and mandibular nerves (V2, V3) and the Gasserian ganglion. Exposure of the caudal ICA (petrous and parapharyngeal) and the infrapetrous area required a medial maxillectomy for exposure and resection of the entire pterygoid process, followed by the Eustachian tube and the fibrocartilaginous tissue of the foramen lacerum. The main anatomical landmarks to this step were V3 and the Eustachian tube. CONCLUSION. During the endoscopic approach to the suprapetrous area, the vidian nerve, the maxillary and mandibular divisions of the trigeminal nerve (V2, V3) and the Gasserian ganglion are the anatomical landmarks to the internal carotid artery and middle cranial fossa. During the endoscopic approach to the infrapetrous area, the fibrocartilaginous tissue of the foramen lacerum, the Eustachian tube and V3 are the landmarks to the internal carotid artery. A stepwise endoscopic endonasal approach provided access to the extracranial internal carotid artery from its cavernous to the parapharyngeal segment down to the level of the nasal fossa floor. The anatomical landmark to the lacerum and horizontal petrous segments of the internal carotid artery is the vidian nerve; to the horizontal petrous segment before the posterior bend and carotid canal, the anatomical landmarks are the mandibular nerve (V3), foramen ovale, fibrocartilaginous tissue of the foramen lacerum and the Eustachian tube; to the parapharyngeal segment, the anatomical landmarks are the posterior trunk of the mandibular nerve (V3) and the Eustachian tube
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Le lambeau-pontage épiploïque : une nouvelle technique de revascularisation pour le sauvetage de membre (étude anatomique, radiologique et expérientale) / Epiploic flow-through flap : a new method of limb salvage : Anatomical, radiological and experimental studySettembre, Nicla 05 September 2014 (has links)
L’incidence de l’ischémie critique est en augmentation depuis ces vingt dernières années. La revascularisation diminue le taux d’amputation. Les plaies ischémiques infectées avec l’exposition des tendons, des os ou des articulations, ne peuvent pas cicatriser avec la revascularisation et le débridement local. La chirurgie, associant un pontage veineux distal ou une recanalisation et un lambeau libre, permet de traiter les pertes de substances et présente un avantage hémodynamique en augmentant le débit du pontage grâce au lit vasculaire ajouté par le lambeau. Nous proposons une nouvelle technique chirurgicale basée sur l’utilisation d’une unité anatomique unique, le lambeau-pontage épiploïque (LPE). Il est composé d’un axe artériel, l’artère gastroépiploïque (AGE), qui procure le greffon, et du grand épiploon, utilisé comme lambeau irrigué par une ou plusieurs branches épiploïques. Le but de ce travail était d’analyser la faisabilité anatomique d’un LPE et de valider le scanner pour l’évaluation préopératoire de l’AGE. Nous avons également évalué les effets hémodynamiques de cette technique et analysé la première expérience clinique. 100 dissections anatomiques ont été réalisées afin de mesurer les diamètres et les longueurs de l’AGE droite et de ses branches, ainsi qu’une radiographie après injection de produit radio-opaque. Pour évaluer la faisabilité préopératoire, nous avons étudié 30 tomodensitométries. Nous avons également exploré les effets hémodynamiques dans le modèle porcin. Notre étude anatomique confirme la faisabilité d’un LPE. La longueur moyenne de l’AGE est de 24,5 cm. Le diamètre proximal moyen est de 3 mm et celui distal est de 1,5 mm. Les données de l’étude radiologique montrent que le scanner peut être utilisé pour le repérage préopératoire de l’AGE et la faisabilité d’un LPE. Les mesures hémodynamiques ont montré que, grâce au lambeau épiploïque, le débit sanguin du pontage augmente et que les résistances distales diminuent. Le LPE est une technique chirurgicale qui doit permettre de repousser les limites de sauvetage de membre dans les conditions de cette pathologie extrême, réalisant une revascularisation distale et une couverture simultanée des pertes de substances chez les patients atteints d’artériopathie. / The incidence of critical limb ischemia increases with the ageing of the population. Often, revascularization decreases the rate of amputation. In some cases, infected wounds with exposure of the tendons, bones or articulations will not heal only with revascularization and local debridement. Surgery combining a distal venous bypass or recanalisation and a free flap can treat those wounds, the vascular bed added by the flap improve the hemodynamic and increases the flow in the bypass. We proposed a new surgical method based on the use of a single anatomical unit, the epiploic flow-through flap (FTF), the gastroepiploic artery (GEA) as the vascular substitute and the greater omentum as the flap. The aim of this work was to analyze the anatomical feasibility of an epiploic BF, and to validate CT scan for preoperative assessment of the suitability of the GEA. We also aimed to evaluate the hemodynamic effects of this technique and to analyze the first clinical experience. 100 anatomical dissections were performed in order to measure the diameters and the lengths of GEA and its branches. An extensive X-ray study was also carried out with injection of a radiopaque product. To evaluate preoperative feasibility of the omental FTF, we studied radiological properties of the GEA on 30 routine CT scans. Finally, we also explored the hemodynamic behavior of this artery and its related flap in porcine models. Our anatomical study confirms the feasibility of a FTF. The average available length of GEA is 245 mm. The average proximal diameter is 3 mm and the distal one is 1.5 mm. Data of radiological study show that CT scan can be used to indicate GEA suitability for an epiploic FTF. The hemodynamic measures showed that thanks to the flap, the blood flow increase in the bypass. Ultimately,we report our first clinical application of the omental FTF for distal lower limb revascularization combined to wound coverage, with successful outcome. Epiploic FTF is a surgical technique, which allows distal revascularization and a simultaneous cover of the limb extremity. This technique can be useful in patients requiring a distal revascularization associated with the coverage of large the wounds
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