Made available in DSpace on 2014-06-11T19:33:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-11-27Bitstream added on 2014-06-13T18:45:21Z : No. of bitstreams: 1 nicodemo_d_dr_jabo.pdf: 529924 bytes, checksum: 436115f2f4f07ab45288d9c5ed7c0e35 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / O cultivo em estufas propicia a obtenção de produtos de excelente qualidade, porém, os agentes polinizadores que contribuem na maximização da produção não são adaptados a ambientes fechados. Utilizando três cultivares tipo Japonês e duas tipo Aodai de pepino (Cucumis sativus L.) foram realizados dois experimentos em três estufas localizadas na USP/Ribeirão Preto. Os objetivos foram estudar a biologia floral das cultivares no que se refere ao número de flores produzidas por planta, período de antese, porcentagem de açúcares do néctar, produção e viabilidade dos grãos de pólen, receptividade do estigma e atratividade de flores e, a importância das abelhas Jataí (Tetragonisca angustula), Iraí (Nannotrigona testaceicornis) e Africanizadas (Apis mellifera) quanto a freqüência das abelhas nas flores, tempo e tipo de coleta e produção de frutos quanto ao peso, comprimento e diâmetro. O manejo das abelhas africanizadas foi estudado e um modelo de núcleo com dois alvados desenvolvido. As flores das cultivares avaliadas têm antese de, aproximadamente, 10 horas. A viabilidade dos grãos de pólen e a receptividade do estigma não são limitantes na polinização de pepino Japonês e Aodai. Os índices de frutificação de pepino Japonês por partenocarpia são altos (78 %), porém há aumento de 19% quando ocorre polinização por abelhas. A cultivar Aodai depende dos insetos para produção de frutos, sendo que flores visitadas até às 10h30 originaram frutos mais pesados. As abelhas Africanizadas se adaptaram a colméia com dois alvados, visitando flores em parte do dia dentro da estufa, promovendo a polinização, e o restante fora, ambiente com maior oferta de recursos. / The cultivation in greenhouses allows obtaining products of excellent quality, however, the pollinators that contribute maximizing the production are not adapted to the indoor environment. Using three Japanese and two Aodai cultivars of cucumber (Cucumis sativus L.) were conducted two experiments in three greenhouses located in USP / Ribeirão Preto. The aims were to study the cultivars floral biology with regard to the number of flowers produced per plant, anthesis period, the percentage of sugars in nectar, production and viability of pollen grains, stigma receptivity and attractiveness of flowers, and the importance of Jataí (Tetragonisca angustula), Iraí (Nannotrigona testaceicornis) and Africanized honeybees (Apis mellifera) with regard to the frequency of bees in the flowers, time and type of collection and fruit weight, length and diameter. The management of Africanized bees has been studied and a hive with two entrances was developed. The anthesis period was approximately of 10 hours. The viability of pollen grains and the receptivity of stigma did not limit the pollination of Japanese cucumber and Aodai. The parthenocarpy fruit set of Japanese cucumber was high (78%), but there is an increase of 19% in fruit set when the flowers were pollinated by bees. The cultivar Aodai depends on the insects for production of fruits. Flowers visited until 10h30 originated the heaviest ones. Africanized bees adapted itself to the hive with two entrances, visiting flowers in part of the day inside the greenhouse, promoting pollination, and the rest outside, the environment with greater supply of resources.

Resumo: O cultivo em estufas propicia a obtenção de produtos de excelente qualidade, porém, os agentes polinizadores que contribuem na maximização da produção não são adaptados a ambientes fechados. Utilizando três cultivares tipo Japonês e duas tipo Aodai de pepino (Cucumis sativus L.) foram realizados dois experimentos em três estufas localizadas na USP/Ribeirão Preto. Os objetivos foram estudar a biologia floral das cultivares no que se refere ao número de flores produzidas por planta, período de antese, porcentagem de açúcares do néctar, produção e viabilidade dos grãos de pólen, receptividade do estigma e atratividade de flores e, a importância das abelhas Jataí (Tetragonisca angustula), Iraí (Nannotrigona testaceicornis) e Africanizadas (Apis mellifera) quanto a freqüência das abelhas nas flores, tempo e tipo de coleta e produção de frutos quanto ao peso, comprimento e diâmetro. O manejo das abelhas africanizadas foi estudado e um modelo de núcleo com dois alvados desenvolvido. As flores das cultivares avaliadas têm antese de, aproximadamente, 10 horas. A viabilidade dos grãos de pólen e a receptividade do estigma não são limitantes na polinização de pepino Japonês e Aodai. Os índices de frutificação de pepino Japonês por partenocarpia são altos (78 %), porém há aumento de 19% quando ocorre polinização por abelhas. A cultivar Aodai depende dos insetos para produção de frutos, sendo que flores visitadas até às 10h30 originaram frutos mais pesados. As abelhas Africanizadas se adaptaram a colméia com dois alvados, visitando flores em parte do dia dentro da estufa, promovendo a polinização, e o restante fora, ambiente com maior oferta de recursos. / Abstract: The cultivation in greenhouses allows obtaining products of excellent quality, however, the pollinators that contribute maximizing the production are not adapted to the indoor environment. Using three Japanese and two Aodai cultivars of cucumber (Cucumis sativus L.) were conducted two experiments in three greenhouses located in USP / Ribeirão Preto. The aims were to study the cultivars floral biology with regard to the number of flowers produced per plant, anthesis period, the percentage of sugars in nectar, production and viability of pollen grains, stigma receptivity and attractiveness of flowers, and the importance of Jataí (Tetragonisca angustula), Iraí (Nannotrigona testaceicornis) and Africanized honeybees (Apis mellifera) with regard to the frequency of bees in the flowers, time and type of collection and fruit weight, length and diameter. The management of Africanized bees has been studied and a hive with two entrances was developed. The anthesis period was approximately of 10 hours. The viability of pollen grains and the receptivity of stigma did not limit the pollination of Japanese cucumber and Aodai. The parthenocarpy fruit set of Japanese cucumber was high (78%), but there is an increase of 19% in fruit set when the flowers were pollinated by bees. The cultivar Aodai depends on the insects for production of fruits. Flowers visited until 10h30 originated the heaviest ones. Africanized bees adapted itself to the hive with two entrances, visiting flowers in part of the day inside the greenhouse, promoting pollination, and the rest outside, the environment with greater supply of resources. / Orientador: Euclides Braga Malheiros / Coorientador: David de Jong / Banca: Leomam Almeida Couto / Banca: Luis Carlos Marchini / Banca: Roque Takahashi / Banca: Jeffrey Frederico Lui / Doutor

Corn is one of the cereals most important, being cultivated in several countries in the world, has a fundamental role in human food and animal, and being it also indispensable in industry. However, the tendency to overcome the productivity of this is a constant challenge. Thus, the objectives of the present study were: characterize families S1, S2 and S3 from landrace corn as to phenological development, thermal demand and flowering synchrony; reveal effects of artificial defoliation in philological maturity on yield components of corn hybrids, in different plant populations. The study 1 (E1) was carried at the farm harvest 2015/2016 in the experimental area of the Universidade Federal de Santa Maria, Campus of Frederico Westphalen (UFSM-FW). For this, 34 segregating families were studied in different generations, belonging to from four populations of landrace corn (Planalto, Dente de ouro, Argentino amarelo and Argentino branco). For this, five commercial hybrids were used as controls. For the study 2 (E2) were used two simple hybrids DKB 240 VT PRO® e DKB 290 VT PRO®). The experiment was carried in the municipality of Redemption/RS, in the harvest 2014/2015, in a randomized block design in a factorial 2x2x2 (hybrids, defoliation and plant population). In E1, the families AAS1F1, DOS3F3, ABS2F2, ABS3F1, PLS1F1, PLS3F1, PLS3F2 and PLS3F3 had flowering synchrony. The duration of vegetative stages of families was relative to the quantity of leaves developed. The families with the high number of leaves demanded high thermal requirement to reach the flowering, indicating that more time is needed to develop a high number of source-organs to maintain a higher biomass. For the E2, the total defoliation in the physiological maturity negatively affects the yield components of the ear, reducing ear length (5.6), the kernel mass per ear (10.9), the ear mass (8.9) and the grain yield (9.5%). The increase in plant population has reduced the number kernel per row (14.4), the kernel weight (22), the kernel mass per ear (21.3), the thousand kernel weight (10.6), the kernel length (3.8), the kernel width (3.9) and grain yield (9.5%). Artificial defoliation in physiological maturity and high plant populations negatively influenced the main components of maize production. The DKB 290 VT PRO® hybrid presents superiority on kernel mass per ear (6.9), ear length (16.3) and kernel length (4%) in relation to DKB 240 VT PRO® hybrid. / O milho é um dos cereais mais importantes, sendo cultivado em diversos países no mundo, pois tem papel fundamental na alimentação humana e animal, sendo também indispensável para indústria. No entanto, a tendência de superar a produtividade deste cereal é um desafio constante. Dessa forma, os objetivos do presente trabalho foram: caracterizar famílias S1, S2 e S3 de milho crioulo quanto ao desenvolvimento fenológico, exigência térmica e sincronia floral; revelar os efeitos da desfolha artificial realizada durante a maturação filológica sobre os componentes de rendimento em híbridos simples de milho submetidos a diferentes densidades de semeadura. O estudo 1 (E1) foi conduzido na safra agrícola 2015/2016 na área experimental da Universidade Federal de Santa Maria, Campus de Frederico Westphalen (UFSM-FW). Para isto, foram estudadas 34 famílias segregantes em diferentes gerações, oriundas de quatro populações de milho crioulo (Planalto, Dente de Ouro, Argentino Amarelo e Argentino Branco). Para isso, cinco híbridos comerciais foram utilizados como testemunhas. Para o Estudo 2 (E2), foram utilizados dois híbridos simples (DKB 240 VT PRO® e DKB 290 VT PRO®). O experimento foi realizado no município Redentora/RS, na safra 2014/2015, em delineamento de blocos casualizados com esquema fatorial 2x2x2 (híbrido, desfolha e população de plantas). Em E1, as famílias AAS1F1, DOS3F3, ABS2F2, ABS3F1, PLS1F1, PLS3F1, PLS3F2 e PLS3F3 tiveram sincronia na floração. A duração dos estádios vegetativos das famílias foi relativo à quantidade de folhas desenvolvidas e as famílias com maior número de folhas demandaram maior exigência térmica para atingir a floração, indicando assim, que maior tempo é necessário para desenvolver maior número de órgãos fonte para atender uma maior biomassa. Para E2, a desfolha total na maturação fisiológica afeta negativamente os componentes de rendimento da espiga, sendo o comprimento de espiga (5,6), a massa de grão por espiga (10,9) e a massa de espiga (8,9) e o rendimento de grãos diminui (9,5%). O aumento na população de plantas reduziu o número de grãos por fileira (14,4), a massa de espiga (22), a massa de grãos por espiga (21,3), a massa de mil grãos (10,6), o comprimento de grãos (3,8), a largura de grãos (3,9) e o rendimento de grãos (9,5%). A desfolha artificial na maturação fisiológica e populações altas de plantas influenciaram negativamente os principais componentes de produção do milho. O híbrido DKB 290 VT PRO® foi superior com a massa de grãos por espiga (6.9), comprimento de espiga (16.3) e comprimento de grãos (4%) em relação ao híbrido DKB 240 VT PRO®.

No description available.

Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat. A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease. Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive. The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.

Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat. A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease. Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive. The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.