O principal objetivo deste trabalho foi o de utilizar um indicador de sustentabilidade conhecido como BAF (Fator Biótopo de Área) no mapeamento de geótopos urbanos da bacia hidrográfica do Córrego Água Espraiada, (Estado de São Paulo SP capital - Brasil), em dois momentos históricos, 1958 e 2011. As unidades da paisagem estudadas enquadraram-se nos níveis de paisagem proposta por Bertrand. A partir dessa classificação realizou-se o mapeamento dos geótopos, e utilizando o BAF fez-se a análise dos coeficientes de impermeabilização das superfícies que cobrem as áreas estudadas. Utilizando essa metodologia é possível a identificação de geótopos incompatíveis à sustentabilidade e assim propor solucões. Essa abordagem pode ser utilizada como ferramenta para um melhor planejamento urbano brasileiro aliado a qualidade ambiental. / The main objective of this thesis it was to evaluate the use of the sustainability indicator named \"BAF\" (Biotope Area Factor) to mapping urban geotopes present in the drainage basin \"Aguas Espraiada\" (São Paulo-SP-Brazil) in two historical moments, 1958 and 2011. The landscape units studied were classified as geotopes as proposed by Bertrand. Considering this classification we mapped the geotopes, and using BAF we did the analysis of the surface waterproofing coefficients that covers the studied area. Using this methodology it is possible to identify the geotopes incompatible with sustainability and propose clever solutions. This approach can be used as a tool for a better Brazilian urban planning associated with environmental quality.

O principal objetivo deste trabalho foi o de utilizar um indicador de sustentabilidade conhecido como BAF (Fator Biótopo de Área) no mapeamento de geótopos urbanos da bacia hidrográfica do Córrego Água Espraiada, (Estado de São Paulo SP capital - Brasil), em dois momentos históricos, 1958 e 2011. As unidades da paisagem estudadas enquadraram-se nos níveis de paisagem proposta por Bertrand. A partir dessa classificação realizou-se o mapeamento dos geótopos, e utilizando o BAF fez-se a análise dos coeficientes de impermeabilização das superfícies que cobrem as áreas estudadas. Utilizando essa metodologia é possível a identificação de geótopos incompatíveis à sustentabilidade e assim propor solucões. Essa abordagem pode ser utilizada como ferramenta para um melhor planejamento urbano brasileiro aliado a qualidade ambiental. / The main objective of this thesis it was to evaluate the use of the sustainability indicator named \"BAF\" (Biotope Area Factor) to mapping urban geotopes present in the drainage basin \"Aguas Espraiada\" (São Paulo-SP-Brazil) in two historical moments, 1958 and 2011. The landscape units studied were classified as geotopes as proposed by Bertrand. Considering this classification we mapped the geotopes, and using BAF we did the analysis of the surface waterproofing coefficients that covers the studied area. Using this methodology it is possible to identify the geotopes incompatible with sustainability and propose clever solutions. This approach can be used as a tool for a better Brazilian urban planning associated with environmental quality.

No description available.

Coordination of gene expression within the cell requires the integrated actions of various multi-protein, gene regulatory complexes. The Mediator and BRG1 Associate Factor (BAF) complexes are large, dynamic regulatory cofactors (COF) that are made up of multiple different submodules, and play key roles in regulating gene expression. Gene-specific regulation requires that transcription factors (TFs) recruit these COF complexes to gene promoters. How separate subdomains in each complex interact with distinct sets of TFs in each cell remains an important question. In this study, to address this question, we sought to apply the nuclear extract protein-binding microarray (nextPBM) technology being developed in our lab to study interactions between TFs and subunits of the Mediator and BAF complexes. To facilitate this, we cloned, expressed and purified subdomains of proteins from the Mediator and BAF complexes. We then used the nextPBM technology to study the interactions of our subdomains with TFs in human macrophages. We identified several new interactions with TFs, and demonstrate the utility of this approach to student TF-COF interaction.

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-01-27T13:40:32Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação - Paulo Henrique Ramos Guimarães - 2014.pdf: 1517515 bytes, checksum: 862883b307a6231b2a7f63d38c9f03ea (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-01-28T11:19:18Z (GMT) No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação - Paulo Henrique Ramos Guimarães - 2014.pdf: 1517515 bytes, checksum: 862883b307a6231b2a7f63d38c9f03ea (MD5) / Made available in DSpace on 2015-01-28T11:19:18Z (GMT). No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação - Paulo Henrique Ramos Guimarães - 2014.pdf: 1517515 bytes, checksum: 862883b307a6231b2a7f63d38c9f03ea (MD5) Previous issue date: 2014-02-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Some spatial analysis methods have been applied in order to mitigate environmental variation. The objective of this study was to evaluate the efficiency of spatial statistical, through the method of Papadakis, relative to the analysis to augmented blocks Federer in the correction of environmental variation. Were evaluated 198 progenies S0:2 of rice and four witnesses for augmented blocks of Federer. Data set were taken in the grain yield (GY, kg ha- 1) and plant height (PH, cm). The data set were subjected to variance analyses and were estimated the genetic and phenotypic parameters. The different approaches (BAF and Papadakis) were compared as to their estimates of genetic and phenotypic parameters. The ranking of adjusted means in the two models analyzed was performed, and calculated the Spearman correlation. There have been improvements in the statistics that depict the experimental accuracy when the spatial analysis was, that affect the estimates of genetic and phenotypic parameters. The use of the Papadakis method yielded fewer iterations compared to BAF for the same value of . Was able to gain direct selection for the AP and PG characters when the Papadakis method was used. With the use of spatial analysis selection was less influenced by the effect of environmental variation. Finally it was found that the spatial analysis methods were effective in the removal of environmental effects highlighting the Papadakis method, indicating that it can provide improvement / Alguns métodos de análise espacial têm sido aplicados objetivando reduzir a variação ambiental. O objetivo deste trabalho foi avaliar a eficiência da análise espacial, por meio do método de Papadakis, em relação ao delineamento de blocos aumentados de Federer na correção da variação ambiental. Foram avaliadas 198 progênies S0:2 de arroz irrigado e quatro testemunhas no delineamento de blocos aumentados de Federer. Os caracteres avaliados foram: produção de grãos (PG, kg ha-1) e de altura de plantas (AP, cm). Foi efetuada análise de variância para os caracteres estudados e estimados os componentes de variância e os parâmetros genéticos e fenotípicos. As diferentes abordagens (BAF e Papadakis) foram comparadas quanto às suas estimativas de parâmetros genéticos e fenotípicos e correlação de Spearman. Houve melhorias nas estatísticas que retratam a precisão experimental quando a análise espacial foi utilizada, isto influenciou as estimativas de parâmetros genéticos e fenótipos. O uso do método de Papadakis apresentou a necessidade de uso de menor número de repetições em relação ao BAF para o mesmo valor de . Houve ganho de seleção direto para os caracteres AP e PG quando o método de Papadakis foi utilizado. Com o uso da análise espacial a seleção foi menos influenciada pelo efeito da variação ambiental. Por fim verificou-se que o método de Papadakis foi eficiente na remoção dos efeitos ambientais, indicando que o mesmo pode proporcionar melhoria na precisão experimental, o que torna o processo seletivo mais eficiente.

No description available.

The present dissertation aims to contribute to the to date more limited findings of studies examining the factor of starting age in second language (L2) phonological learning in a formal instruction setting. Thus, its main objective was to assess the effects of starting ages of 8, 11, 14, and 18+ years on segmental perception and production of English as a foreign language (FL) in a strictly instruction-classroom context. Native Spanish and Catalan speakers learning English through schooling solely, who also differed in the amount of formal exposure to the FL (200, 416 and 726 hours of formal instruction in English), comprised the subject population under examination. All subjects were part of a long-term research project on the age factor in the acquisition of English as an FL in a formal instruction context - the Barcelona Age Factor (BAF) project - conducted at the Universitat de Barcelona over a period of 9 years (1995-2004). For the current study, subjects performed a same-different (AX) discrimination task and a production (word imitation) task.Results showed that in a formal learning environment starting age of 8 led to a better perception of English sounds in the long run than starting ages of 11, 14, and 18+. However, starting age effects on English segmental production both in the short- and in the long-term were inconclusive, as assessed by native English listeners. Similarly, exposure effects yielded mixed results as to more accurate perception and production of FL sounds. By contrast, a rather conclusive finding was obtained concerning subjects' first language (L1) dominance, namely being a Spanish or Catalan dominant speaker did not result in significantly more accurate English sound perception or production. Furthermore, female subjects were reported to produce English segments at more native-like levels than male subjects at all starting ages and with various degrees of formal exposure. Finally, it should be mentioned that the starting age effects observed in the short- and long-term (as conceived in the large research project design) provide invaluable evidence for characterising rate of acquisition of target language (TL) segmental perception and production by Catalan and Spanish native speakers (NSs) in a formal instruction learning context. The current dissertation concludes by suggesting a number of implications for further research.

Submitted by JAQUELINE CARDOSO RIBEIRO null (jack_0811@hotmail.com) on 2016-09-03T18:07:20Z No. of bitstreams: 1 Dissertação Jaqueline Ribeiro_final_02_09_16.pdf: 4909806 bytes, checksum: 51cd2d5eea7b87d58fc6e979fbed6dfb (MD5) / Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2016-09-06T18:31:55Z (GMT) No. of bitstreams: 1 ribeiro_jc_me_bauru.pdf: 4909806 bytes, checksum: 51cd2d5eea7b87d58fc6e979fbed6dfb (MD5) / Made available in DSpace on 2016-09-06T18:31:55Z (GMT). No. of bitstreams: 1 ribeiro_jc_me_bauru.pdf: 4909806 bytes, checksum: 51cd2d5eea7b87d58fc6e979fbed6dfb (MD5) Previous issue date: 2016-07-20 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Esta pesquisa foi desenvolvida em uma Estação Compacta de Tratamento de Efluente (ECTE) composta por reator Upflow Anaerobic Sludge Blanket (UASB) seguido de Biofiltro Aerado Submerso (BAS), localizada no município de Uru-SP e foi conduzida em parceria com a Sabesp. As ECTEs podem ser uma ótima alternativa para a implantação de um sistema descentralizado que possa atender pequenas comunidades, no entanto, tais sistemas ainda são pouco utilizados para o tratamento municipal de efluentes sanitários e ainda há pouca experiência com relação à sua operação, portanto esta pesquisa teve como objetivo avaliar a eficiência de tal sistema e realizar seu acompanhamento operacional, e através dos resultados obtidos apresentar diretrizes para sua melhor operação, proporcionando um efluente final de melhor qualidade. Este acompanhamento foi realizado através de análises físico-químicas com duração de 16 semanas e foram feitas coletas semanais de cada etapa do tratamento e coletas mensais do perfil de lodo do reator UASB. Através deste acompanhamento foi possível estimar diretrizes para o volume e periodicidade de descarte de lodo uma vez que havia um histórico de problemas em relação a Sólidos Sedimentáveis (SS) na ECTE e apresentar sugestões de melhorias para o sistema em relação às questões de projeto, dimensionais e operacionais. Durante a pesquisa, o sistema mostrou-se bastante eficiente principalmente em relação às remoções de Demanda Bioquímica de Oxigênio (5 dias) (DBO5) e Demanda Química de Oxigênio (DQO) filtrada com valores médios de no mínimo 91% e 85% respectivamente. Também acredita-se que o monitoramento contribuiu para uma melhora geral do sistema, pois durante a sua realização foi obtida a Licença de Operação (LO) da ECTE que até então estava sendo operada com uma Licença de Operação a Título Precário (LOTP), o que mostra que tratamentos descentralizados, tais como ECTEs são boas alternativas para pequenos municípios e podem atender os padrões de eficiência exigidos pela legislação desde que sejam feitos um acompanhamento operacional e manutenção mínimos para o bom funcionamento do sistema. / This research was developed in a Compact Wastewater Treatment Plant (CWWTP) composed of Upflow Anaerobic Sludge Blanket (UASB) reactor followed by Submerged Aerated Biofilter (BAF), located in the municipality of Uru-SP and was conducted in partnership with Sabesp. The CWWTPs can be a great alternative to the implementation of a decentralized system that can serve small communities, however, such systems are still little used for municipal treatment of sewage and there is little experience regarding its operation, so this search aimed to evaluate the efficiency of such a system and perform its operational monitoring, and through the results provide guidelines for its best operation, providing a final effluent of better quality. This monitoring was carried out through physical-chemical analyzes during 16 weeks and weekly collections were made of each stage of the treatment and monthly collections of UASB reactor sludge profile. Through this monitoring it was possible to estimate guidelines for volume and intervals of sludge disposal since there was a history of issues regarding Sedimentable Solids (SS) in the CWWTP and to present suggestions for improvements in the system in relation to design issues, dimensional and operational. During the search, the system proved to be very efficient especially in relation to Biochemistry Demand Oxygen (5 days) (BOD5) and Chemical Oxygen Demand (COD) filtered removals with average values of at least 91% and 85% respectively. It is also believed that the monitoring has contributed to an overall improvement of the system, because during its performance the Operating License (OL) of the CWWTP was obtained ECTE, which until then was being operated with an Operating License to Precarious Title (OLPT), which shows that decentralized treatments, such as CWWTPs are good alternatives for small cities and can meet the efficiency standards required by law since an operational monitoring and minimum maintenance are done for the proper functioning of the system.

No description available.

(EN) Humans and cells in their bodies are exposed to various mutagens in their lifetime that cause DNA damage and mutations, which affect the biology and physiology of the target cell, and can lead to the expansion of an immortalized cell clone. Genome-wide massively parallel sequencing allows the identification of DNA mutations in the coding sequences (whole exome sequencing, WES), or even the entire genome of a tumour. Mutational signatures of individual mutagenic processes can be extracted from these data, as well as mutations in genes potentially important for cancer development ('cancer drivers', as opposed to 'passengers', which do not confer a comparative growth advantage to a cell clone). Many known mutational signatures do not yet have an attributed cause; and many known mutagens do not have an attributed signature. Similarly, it is estimated that many cancer driver genes remain to be identified. This Thesis proposes a system based on immortalization of mouse embryonic fibroblasts (MEF) upon mutagen treatment for modelling of mutational signatures and identification and testing of cancer driver genes and mutations. The signatures extracted from WES data of 25 immortalized MEF cell lines, which arose upon treatment with a variety of mutagens, showed that the assay recapitulates the...