The process of photosynthesis begins with a capture of sunlight and its quick conversion into the chemical energy. Both these primary processes take place in a specially designed photosynthetic apparatus that is present in cells of all photosynthetic organisms. In green sulfur bacteria the apparatus consists of a massive light-harvesting antenna (chlorosome), intermediate antennas (baseplate complex and FMO proteins) and of the reaction center, where the conversion into the chemical energy occurs. The energy flow through the apparatus can be monitored by time-resolved spectroscopy techniques. Coherent two-dimensional electronic spectroscopy (2DES) in one of such techniques, which combines high temporal and spectral resolution, and therefore it is especially well suited for studying multichromophoric systems such as photosynthetic apparatus. This thesis describes the principles of the 2DES technique and outlines the basic facts about the photosynthetic apparatus of green sulfur bacterium Chlorobaculum tepidum. Finally, it summarizes the investigation of the photosynthetic machinery using 2DES. Results presented in this thesis provide new insights into the exciton diffusion and vibrational coherences within chlorosomes, excitonic structure of the baseplate and the overall energy flow through the entire...

The goal of diploma thesis was a comparison of potable water indicators in representative areas of the south Bohemia. Monitored period had been from January 2012 till December 2014. Monitored parameters were microbiological indicators (Escherichia coli, coliform microbes, number of colonies at 22°C, number of colonies at 36°C, organoleptic indicators (color, turbidity, smell, taste). Physical and chemical indicators (ammonium ions, nitrates, nitrites, chemical consumption of oxygen, chlorides, conductivity, manganese, pH, sulphates, calcium and magnesium, iron). From the total complex of 1165 data, there were 23 values over the limit (1,97%) of evaluation of all monitored quality indicators (microbiological, organoleptic, physical and chemical). In evaluation of microbiological indicators, there were 6 values (1,97%) over the limit, from total 304 data. In organoleptic indicators, there was 1 value (0,44%) over the limit, from total 227 data. In evaluation of physical and chemical indicators, there were 16 values (2,52%) over the limit, from total 634 data. In total, there are 23 values which don´t meet determined limits from The Regulation No. 252/2004 and EU directives. There are these values of limits - number of colonies at 22°C, number of colonies at 36°C, turbidity, manganese, calcium and iron.

Cílem této diplomové práce je provést analýzu vybraných ukazatelů kvality pitné vody a posouzení faktorů, které ji ovlivňují. V první části jsou zachyceny vybrané mikrobiologické ukazatele, tak jak je popisuje literatura, včetně platných norem a vyhlášek. Druhá část popisuje průběh provádění vlastní analýzy, z poskytnutých materiálů akreditovanou laboratoří společnosti AGRO-LA, spol. s r. o., až po vyhodnocení získaných výsledků.

Recent research shows, that gut microbiome can influence various functions of the organism and is able to communicate with the brain. The data also show that changes in the composition of gut microbiome can influence behavior and stress reactions and vice versa, psychological state of the organism can cause changes in gut microbiome. The aim of this master's thesis was to examine changes of HPA activation and local metabolism of glucocorticoids caused by stress in the presence or absence of gut microbiome. We therefore used germ-free mice and studied the effect of stress in pituitary, adrenal gland, colon and spleen. We found that, stress has different impact on gene expression in adrenal gland, colon and spleen in the presence or absence of gut microbiome. In contrast, there wasn't any significant effect of stress on pituitary in germ free mice and their conventionaly colonized counterparts.

The aim of the diploma thesis is to characterize the adsorption properties of probiotic bacteria. Characterization was performed using of designed and optimized spectrophotometric method of determination of adhesion of probiotic bacteria to mucin as the main viscoelastic component of mucus. In order to provide a more detailed description of the adhesion interactions involved in the adhesion of probiotic bacteria to mucin, the viability of selected probiotic bacterial strains Lactobacillus rhamnosus CCM 1825, Lactobacillus plantarum CCM 7039, Lactobacillus delbrueckii subsp. bulgaricus CCM 7190, Lactobacillus acidophilus CCM 4833, Lactobacillus casei CCM 4798, Bifidobacterium breve CCM 7825, Bacillus coagulans CCM 2658 and a potential probiotic strain of Lactobacillus zeae CCM 7069. Moreover, bacterial adhesion to carbohydrates was also determined for all the bacteria tested. The last proposed and optimized technique was dynamic and electrophoretic light scattering characterizing surface properties such as -potential, average size distribution and isoelectric point. From this method, information was obtained on aggregation of bacterial cells and on electrostatic interactions. The combination of these methods was used as a complex tool to characterize adhesion of the tested bacterial cultures as a very specific, sensitive and key parameter of a successful probiotic microorganism that is influence by the multiple effects.

This master thesis describes the design of heating in a new one-storey nursery school in Kuřim. The heating systém is designed as a low-temperature systém with a forced circulation of water and a horizontal piping. Three kinds of radiators are used in the design. The panel radiators, tube radiators and free-standing convectors. In this project there are two varinats of the heat source for buliding. The first variant is two gas condensing boilers and the second variant is two heat pumps of an air/water split design. The disign includes air heating in the unit of air conditioning and hot water preparation. The experimental part of the master thesis is focused on microbial microclimate in buildings and radiators contamination by microorganisms. It contains four different experiments, their realization, results and evaluation.

Antimicrobial substances, or bacteriocins are substances produced by probiotic lactic acid bacteria. They have a positive effect on the gastrointestinal tract of humans and are especially suitable for the food, but also the pharmaceutical industry. The aim of the thesis was the molecular identification of lactic acid bacteria of the Lactobacillus genus, species and their subsequent inclusion PCR method. Using the PCR method were tested also genes responsible for the production of bacteriocins. To confirm the production of bacteriocins has been selected the microbiological method, agarose droplet spot-test. In the present study also included the bioinformatics part to assess the specificity and non-specificity of the primers using in Primer-BLAST program.

The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...

The diploma thesis "Media terrorism via bacteria E.coli" puts the so-called cucumber affair from Summer 2011 into wider context and aims to discover the main attributes of contemporary risks and define the way they are presented in the media. Misguiding media releases and the inability to identify the source of the bloody diarrhea outbreak in Germany caused by E.coli bacteria have collectively had a significant impact on consumer behavior and economic stability on a global level. Most people are dependent on government and expert authorities due to the fact that they cannot recognize what represents a realistic threat and what serves as a basis for media sensation. This is why this thesis studies the social constructionist theory, basic media routines and practices as well as theoretical concepts that support the production of pseudo-events. A content analysis of selected Czech dailies and weeklies was executed in order to enable a comparison of the language that has been common for all "pandemics" in the 21st century.

The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.