Considerable controversy exists in the world with respect to coastal quality. A multidisciplinary project was initiated to examine the health effects of bathing in sewage contaminated coastal waters, using a popular beach resort, Whitmore Bay, close to the cities of South Wales; and to explore ways of measuring public perception of coastal pollution at selected beaches in South Wales including Whitmore Bay, Langland Bay and Cefn Sidan. The research also investigated the regulatory framework responsible for the sustainability of coastal tourism and the effectiveness of beach award flags as marketing tools in the promotion of resorts. Current legislation addresses coastal pollution in terms of physical health criteria with little regard given to aesthetic quality of sea/landscape and psychological well-being of the beach user. It is necessary to overcome the dichotomised approach to beach management by crossing the boundaries between the physical and social sciences in order to take an holistic view of the coastal scene, accounting for environmental, political, economic and social aspects. An epidemiological/microbiological investigation was conducted at Whitmore Bay during the summer of 1995. Statistical modelling. using Linear Logisitic Regression, indicated swimmers to significantly increase their chance of contracting an illness in comparison to non-swimmers and also identifed non-water related factors to have a confounding effect; no interaction was observed. These findings were in congruence with other major studies. Beach questionnaires were distributed to elicit information on the activities, health and socio-demographic characteristics of the subjects during the day of the survey (n=1276). A telephone interview schedule was utilised 10 days post the beach survey to investigate the differential in illness rates between the cases and controls (n=585). Water sampling was carried out on the days of the health risk survey. Although, high counts of both Ecoli and faecal streptococci were recorded, reaching an average of 3400 and 440 per 100ml respectively, no dose response relationship was observed between morbidity rates and bacterial indicator density. A semi-structured questionnaire was employed to obtain data on beach user perception to coastal pollution and beach award schemes for both the 1995 and 1996 surveys. The 1995 questionnaire served a dual approach running simultaneously with the epidemiological-microbiological analysis (n=1276). The 1996 survey questionnaire was developed from the original 1995 questionnaire. and distributed at an additional two beaches in South Wales, Langland Bay and Cefn Sidan, (n=821). Results of both surveys showed that beach users were acutely aware of coastal pollution both land based and marine and suggested that public awareness of the different beach award schemes is low. Of the different types of award systems included on the questionnaire, the European Blue Flag Award gained highest recognition (26-30%), but even those that identified with it often had a misunderstanding of its true meaning. If consumers misinterpret the meaning of the flag which flies on a designated beach. then the designation of the beach will do little to offset consumers' concerns about health risks.

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Made available in DSpace on 2014-06-11T19:22:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-06-22Bitstream added on 2014-06-13T19:48:42Z : No. of bitstreams: 1 traficante_dp_me_jabo.pdf: 1568154 bytes, checksum: e9230b2e4ff60845b3fe6ffebb26eea6 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Universidade Estadual Paulista (UNESP) / O município de Botucatu está localizado na região centro-sul do Estado de São Paulo, a 230 km da capital, com altitudes variando de 400 a 950 metros. Essa geomorfologia propicia a formação de quedas d’água, cujo relevo chamado “Cuesta” é divisor de águas entre a Bacia do Rio Paranapanema e a Bacia do Rio Tietê. É na microbacia do Alto Rio Capivara (Bacia do Tietê) onde está situado o Parque Natural Municipal Cachoeira da Marta, Unidade de Conservação de Proteção Integral, conhecido como “Parque da Marta”, tendo como principal atrativo a Cachoeira da Marta (38 metros de queda d’água), sendo um local muito procurado para a prática de atividades de lazer e ecoturismo. A Cachoeira é abastecida pelas águas do Córrego do Roseira, tendo como principal afluente o Córrego do Canela, que ao se juntarem, formam o Rio Capivara. O presente estudo teve como objetivos avaliar as condições limnológicas de balneabilidade da Cachoeira da Marta em interface com as possíveis fontes potenciais de poluição para propor medidas de recuperação e conservação através da analise dos parâmetros físico-químicos (TºC da água, pH, Condutividade elétrica, Oxigênio dissolvido, Nitrogênio total e Fósforo total) e microbiológicos (Coliformes totais e termotolerantes). As coletas de água foram bimensais durante 12 meses, de Junho/2009 a Maio/2010, em 5 pontos amostrais diferentes, sendo 3 pontos (P1, P2 e P3) no Córrego do Roseira, 1 ponto na junção dos Córregos do Roseira e Canela (P4) e 1 ponto no Córrego do Canela (P5). Os P2, P3 e P4 se localizavam dento do Parque da Marta. Para a comparação das médias nos pontos amostrais foi realizada a analise de variância (ANOVA) seguidas do Teste de Tukey (Tukey’s Studentized Range – HSD) para as variáveis que apresentaram distribuição normal e o teste não paramétrico de Kruskal-Wallis para as variáveis... / Botucatu is a municipality located in the central south region of Sao Paulo State, 230 km away from de the capital at altitudes ranging from 400 to 950 meters. The landforms in this region favor the formation of waterfalls; the relief, called “Cuesta” is a water divisor for the watersheds of Paranapanema and Tiete Rivers. In the micro-watershed of Capivara Highest River (Tiete watershed) is located the Marta’s Waterfall Natural Park, a protected area, know as “Marta’s Park”, where the Marta’s waterfall (38 meters by heigh) is the principal atractive and have been searched for tourism and leisure activities. The waterfall is supply by the Roseira River and his principal tributary is the Canela River and when they to join up, they transformer in the Capivara River. This study aimed to evaluate the limnological conditions for the bathing water quality by the Marta’s Waterfall associated with the possible potential source by pollution and suggest conservation and recovery measures through the physicochemical (water temperature (ºC), pH, eletric condutivity, dissolved oxigen, total nitrogen, total phosporus) and microbiological parameters (total and thermotolerants coliforms). The water samples were bimonthly collected for 12 months, between June/2009 ande May/2010, in different sites over the streams , in 5 diffrents collections sites, 3 sites (P1, P2 e P3) are inside in Roseira River, 1 site in the joiing at Roseira and Canela Rivers (P4) e 1 site located in Canela River (P5). For comparison of medias in the collections sites was realized the variance analisys (ANOVA) followed by the Tukey’s Studentized Range (HSD) for the medias that showed normal distribution and the nonparametric Kruskal-Wallis test for the medias that doesn’t showed normal distribuition, both in the 5% significance level. In the collection sites 1 and 5 were founded medias for dissolved oxigen... (Complete abstract click electronic access below)

<p>The aim of this paper is to explain and compare non-compliance of two EU-directives, “the quality of bathing water” and “the working time directive”. This study answers the questions: Why didn’t Sweden implement the directive on bathing water quality and the working time directive correctly? Are the reasons for non-compliance the same or different in the two cases? The paper is designed as a case study and with an explanatory attempt we explain why the two directives weren’t implemented correctly in Sweden. The theoretical approach is based on both general- and EU-specific implementation theories. The reason why the directive on quality of bathing water wasn’t implemented correctly was firstly because of the differences between the Swedish political contents and the EU-directive’s, secondly the vague formulation that caused problems with understanding how to implement the directive. Third and lastly the implementation suffered from the lack of administrative resources. The working time directive had problems with the political contents and the resistance from the trade union and government, which caused delays with the implementation of the directive. By studying these two cases this paper concludes that there is only one point of similarity between the cases, i.e. the political contents.</p>

Orientador: Edmir Daniel Carvalho / Banca: Lucia Helena Sipauba Tavares / Banca: José Pedro Serra Valente / Resumo: O município de Botucatu está localizado na região centro-sul do Estado de São Paulo, a 230 km da capital, com altitudes variando de 400 a 950 metros. Essa geomorfologia propicia a formação de quedas d'água, cujo relevo chamado "Cuesta" é divisor de águas entre a Bacia do Rio Paranapanema e a Bacia do Rio Tietê. É na microbacia do Alto Rio Capivara (Bacia do Tietê) onde está situado o Parque Natural Municipal Cachoeira da Marta, Unidade de Conservação de Proteção Integral, conhecido como "Parque da Marta", tendo como principal atrativo a Cachoeira da Marta (38 metros de queda d'água), sendo um local muito procurado para a prática de atividades de lazer e ecoturismo. A Cachoeira é abastecida pelas águas do Córrego do Roseira, tendo como principal afluente o Córrego do Canela, que ao se juntarem, formam o Rio Capivara. O presente estudo teve como objetivos avaliar as condições limnológicas de balneabilidade da Cachoeira da Marta em interface com as possíveis fontes potenciais de poluição para propor medidas de recuperação e conservação através da analise dos parâmetros físico-químicos (TºC da água, pH, Condutividade elétrica, Oxigênio dissolvido, Nitrogênio total e Fósforo total) e microbiológicos (Coliformes totais e termotolerantes). As coletas de água foram bimensais durante 12 meses, de Junho/2009 a Maio/2010, em 5 pontos amostrais diferentes, sendo 3 pontos (P1, P2 e P3) no Córrego do Roseira, 1 ponto na junção dos Córregos do Roseira e Canela (P4) e 1 ponto no Córrego do Canela (P5). Os P2, P3 e P4 se localizavam dento do Parque da Marta. Para a comparação das médias nos pontos amostrais foi realizada a analise de variância (ANOVA) seguidas do Teste de Tukey (Tukey's Studentized Range - HSD) para as variáveis que apresentaram distribuição normal e o teste não paramétrico de Kruskal-Wallis para as variáveis... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Botucatu is a municipality located in the central south region of Sao Paulo State, 230 km away from de the capital at altitudes ranging from 400 to 950 meters. The landforms in this region favor the formation of waterfalls; the relief, called "Cuesta" is a water divisor for the watersheds of Paranapanema and Tiete Rivers. In the micro-watershed of Capivara Highest River (Tiete watershed) is located the Marta's Waterfall Natural Park, a protected area, know as "Marta's Park", where the Marta's waterfall (38 meters by heigh) is the principal atractive and have been searched for tourism and leisure activities. The waterfall is supply by the Roseira River and his principal tributary is the Canela River and when they to join up, they transformer in the Capivara River. This study aimed to evaluate the limnological conditions for the bathing water quality by the Marta's Waterfall associated with the possible potential source by pollution and suggest conservation and recovery measures through the physicochemical (water temperature (ºC), pH, eletric condutivity, dissolved oxigen, total nitrogen, total phosporus) and microbiological parameters (total and thermotolerants coliforms). The water samples were bimonthly collected for 12 months, between June/2009 ande May/2010, in different sites over the streams, in 5 diffrents collections sites, 3 sites (P1, P2 e P3) are inside in Roseira River, 1 site in the joiing at Roseira and Canela Rivers (P4) e 1 site located in Canela River (P5). For comparison of medias in the collections sites was realized the variance analisys (ANOVA) followed by the Tukey's Studentized Range (HSD) for the medias that showed normal distribution and the nonparametric Kruskal-Wallis test for the medias that doesn't showed normal distribuition, both in the 5% significance level. In the collection sites 1 and 5 were founded medias for dissolved oxigen... (Complete abstract click electronic access below) / Mestre

Orientador: Maurício Augusto Leite / Banca: Fernando Braz Tangerino Hernandez / Banca: Eduardo von Sperling / Resumo: O presente estudo teve por objetivo a determinação semanal da balneabilidade, mensal do índice de qualidade da água (IQA) e do bioensaio de toxicidade aguda do sedimento (Outubro de 2004 à outubro de 2005). Os pontos monitorados foram às praias Catarina (P1), Marina (P2) e Recanto das Águas (P3), que contam com um grande afluxo de pessoas em períodos de verão. O método para a avaliação do número de coliformes fecais e totais tanto na água quanto no sedimento foi o Chromocult. Para a balneabilidade, os resultados para a água evidenciaram que, segundo a Resolução CONAMA 274/00, as praias do reservatório oferecem uma qualidade de água de "Muito Boa" à "Excelente" em pelo menos 70% do ano. Onde sua qualidade está condicionada ao nível do reservatório, sendo que no mês de abril, com a elevação do nível da água, sua classificação foi imprópria nas semanas amostradas. Já a elevação no número de coliformes totais pode estar relacionada com a atividade de pecuária extensiva inserida no entorno do reservatório, que com a chegada das chuvas pode carrear esse material para o corpo d'água. Os resultados de coliformes fecais e totais para o sedimento mantiveram-se baixos em todas as estações analisadas durante o período amostrado. O índice de qualidade da água é composto das variáveis: temperatura... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The present study had for objective the weekly bathing water quality, montly determination of the Water Quality Index (WQI), and the acute toxicity bioassays in the sediment (from october 2004 to october 2005). The monitored points were Catarina (P1), Marina (P2) beaches and Recanto das Águas (P3), which has a great affluence of people in the summer. The method for the evaluation of the number of fecal and total coliforms in the water and sediment it was Chromocult. The bathing water quality results showed, according to Resolution CONAMA 274/00, that the beaches of the reservoir presented a bathing water quality varying from "Very Good" to "Excellent" in at least 70% of the year. Where your quality was conditioned to the level of the reservoir, in April, with the water level elevation, its classification was "Inappropriate". The number of total coliforms can be related to the extensive cattle breeding activity all around reservoir. With the rainy season, the material produced by such an activity can be carried to the body water. The fecal and total coliforms data on sediment remained low in all sampling stations. The water quality index (WQI) is composed of the variables: temperature, pH, turbidity, fecal coliforms, BOD5, dissolved oxygen percentage saturation, total nitrogen, total phosphorus and total solids... (Complete abstract click electronic access below) / Mestre

Made available in DSpace on 2014-06-11T19:29:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-08-22Bitstream added on 2014-06-13T19:26:42Z : No. of bitstreams: 1 vasilio_vaa_me_ilha.pdf: 1318052 bytes, checksum: 8b46e1ce70e2e6b147802e677b0544e9 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente estudo teve por objetivo a determinação semanal da balneabilidade, mensal do índice de qualidade da água (IQA) e do bioensaio de toxicidade aguda do sedimento (Outubro de 2004 à outubro de 2005). Os pontos monitorados foram às praias Catarina (P1), Marina (P2) e Recanto das Águas (P3), que contam com um grande afluxo de pessoas em períodos de verão. O método para a avaliação do número de coliformes fecais e totais tanto na água quanto no sedimento foi o Chromocult. Para a balneabilidade, os resultados para a água evidenciaram que, segundo a Resolução CONAMA 274/00, as praias do reservatório oferecem uma qualidade de água de Muito Boa à Excelente em pelo menos 70% do ano. Onde sua qualidade está condicionada ao nível do reservatório, sendo que no mês de abril, com a elevação do nível da água, sua classificação foi imprópria nas semanas amostradas. Já a elevação no número de coliformes totais pode estar relacionada com a atividade de pecuária extensiva inserida no entorno do reservatório, que com a chegada das chuvas pode carrear esse material para o corpo d'água. Os resultados de coliformes fecais e totais para o sedimento mantiveram-se baixos em todas as estações analisadas durante o período amostrado. O índice de qualidade da água é composto das variáveis: temperatura... / The present study had for objective the weekly bathing water quality, montly determination of the Water Quality Index (WQI), and the acute toxicity bioassays in the sediment (from october 2004 to october 2005). The monitored points were Catarina (P1), Marina (P2) beaches and Recanto das Águas (P3), which has a great affluence of people in the summer. The method for the evaluation of the number of fecal and total coliforms in the water and sediment it was Chromocult. The bathing water quality results showed, according to Resolution CONAMA 274/00, that the beaches of the reservoir presented a bathing water quality varying from Very Good to Excellent in at least 70% of the year. Where your quality was conditioned to the level of the reservoir, in April, with the water level elevation, its classification was Inappropriate. The number of total coliforms can be related to the extensive cattle breeding activity all around reservoir. With the rainy season, the material produced by such an activity can be carried to the body water. The fecal and total coliforms data on sediment remained low in all sampling stations. The water quality index (WQI) is composed of the variables: temperature, pH, turbidity, fecal coliforms, BOD5, dissolved oxygen percentage saturation, total nitrogen, total phosphorus and total solids... (Complete abstract click electronic access below)

Ett avloppsvatten innehåller olika föroreningar som påverkar recipientens vattenkvalitet. En förorening somreningsverken inte är optimerade för att reducera är mikroorganismer, som i recipienten påverkar bland annatbadvattenkvalitén. Syftet med examensarbetet var att ta reda på hur utvalda processteg i ett reningsverkpåverkar utgående halter av mikroorganismer, hur utsläppen av mikroorganismer kan övervakas och hur mikroorganismernapåverkar recipientens badvattenkvalitet. Examensarbetet har uppfyllt syftet genom att undersökahur biologiska reningssteg reducerar indikatororganismerna E.coli och intestinala enterokocker jämfört medslutreningsalternativen kontinuerligt sandfilter samt skivfilter. Provtagning genomfördes på två reningsverk ochresultatet analyserades för att besvara frågan. Andra kvalitetsparametrar i avloppsvattnet samt flöde, lufttemperaturoch nederbörd har jämförts med utgående koncentration av indikatororganismerna från reningsverk föratt undersöka om det finns några samband som går att använda för att uppskatta det mikrobiologiska utsläppet.För undersökningen har de statistiska metoderna Kendalls Tau och Partial Least Squares använts. En litteraturstudiepå hur implementering av läkemedelsrening med ozon kan desinficera avloppsvattnet, vilken doseringsom krävs och vad den bör baseras på har genomförts. Efter avloppsutsläpp i en recipient sprids mikroorganismernamed vattnets strömmar och mikroorganismerna dör normalt av med tiden. Denna avdödandeprocesskan beskrivas med en ekvation av första ordningens kinetik. Ekvationens känslighet för variation i variablernasolinstrålning, temperatur, siktdjup och tid har undersökts. Ekvationen har även använts för en fallstudie av avloppsutsläppvid bräddning och hur det påverkar en badplats nedströms. För fallstudien fanns information omflödestid, utspädning och nedbrytningsvariablerna att tillgå. Undersökningen visar att biologisk rening med biobädd reducerar indikatororganismerna effektivast av de undersöktareningsstegen. Sandfiltrets reduktionsförmåga var god men skivfiltret presterade sämre. Från provtagningenframkom också att koncentrationen av E.coli är konsekvent högre och reduceringen sämre än motsvarandeför intestinala enterokocker. Indikatororganismernas koncentration i utgående avloppsvatten är enligtkorrelationsanalysen slumpmässig och inget signifikant och starkt samband fanns till de undersökta parametrarna. På grund av detta är uppskattning av mikrobiologiska utsläpp med hjälp av mätning av andra parametrarinte tillförlitlig. Den genomförda litteraturgranskningen av ozondosering visar att dosen som krävs för desinfektionav avloppsvatten behöver anpassas för varje reningsverk samt att resultat från forskningen går isär omhur doseringen bör optimeras. I recipienten har siktdjupet störst betydelse för nedbrytningen och recipientensegenskaper är viktigare än utsläppets storlek för vattenkvalitén enligt den modell som använts. Fallstudien visadeatt även vid bräddning på reningsverket hinner mikroorganismerna spädas ut och brytas ner tillräckligt föratt badvattenkvalitén inte ska klassas som otjänlig vid närliggande badplats. / Depending on the efficiency of the processes used in a wastewater treatment plant, the recipient receives watercontaining various concentrations of microorganisms. These microorganisms might infect people using therecipient for recreational purposes. The thesis aims to investigate how some process choices affect the concentrationsof microorganisms in sewage and how the concentration can be monitored with the use of other waterquality measurements. Lastly the microbial degradation after discharge in a lake was investigated. Process choiceswere evaluated through sampling before and after two biological treatment steps, one sand filter facilityand one disc filter facility. The obtained results demonstrate that among the investigated alternatives, a biologicalstep using biofilm is the most efficient for removing microorganisms, followed by sand filtration and leastefficient with high variability was disc filtration. The literature review suggests that the use of ozonation forreducing pharmaceutical residues could be efficient in reducing microorganisms, but the required dosage is notuniformly established in previous research. Statistical analysis on water quality measurements with Kendall’sTau and Partial Least Squares did not appear to be a good substitute to use for monitoring and prediction ofbacterial concentrations. The water quality of the recipient, especially the transparency for UV-light, is moreimportant for maintaining good bathing water quality than the outflow concentration of microorganisms in thesewage. In a small case study, it was shown that an official bathing site is not at risk for acute microbiologicalcontamination even when extreme rainfalls causes overflow in the upstream waste water treatment plant.

Objective: National and supranational directives (EU-bathing water directiv, WHO directives) exist to examine the bathing-water quality with concern to public health criteria. E.coli, Intestinal enterococci and Enteroviruses serve as indicators for the contamination of a water with pathogenic bacteria and viruses. As an valuable indicators for the viral contamination bacteriophages are discussed. The aim of this work is the evaluation of somatic and F+ RNA coliphages as indicators of a contamination with enteric viruses considering a lignite mining lake as example. Approach: In weekly intervals, the concentration of bacterial and viral indicators, as well as the presence or absence of enteric viruses in the mining lake &amp;quot;Werbeliner See&amp;quot; (central German lignite mining area), in the flooding water and in the sewage plant Leipzig-Rosental, which significantly influences the flooding water, was examined. The paramters E.coli, Intestinal enterococci and spores of Clostridium perfringens serve as indicators for the hygienic state. As possible indicators of a viral contamination, somatic and F+ RNA coliphages were examined. Several hygienic relevant enteric viruses (Entero-, Noro-, Astro-, Adeno-, Rota- and Hepatitis A-viruses) were detected by means of adequate molecular biological methods and enumerated. Results: 1. The sewage plant Leipzig-Rosental eliminated fecal bacteria and bacteriophages very efficiently (about two logs), however the reduction of E.coli and enterococci was significant higher then the reduction of Cl.perfringens-spores and bacteriophages. The purification capacity benefits from the application of chemical precipitants (Fe (III) cloride / sulfate). 2. The reduction of genomes of Noro-, Astro- and Enteroviruses by the sewage plant was in the range of one to two logs. 3. All examined viruses could be detected in the inflow and the outflow of the sewage plant during the time of examination. 4. The concentration of fecal bacteria in the lake Werbeliner See was very low. The requirements by the EU-bathing water directive (76/160/EWG) with 2000 . (100 ml)-1 (E.coli, imperial value) and 100 . (100 ml)-1 (enterococci, guide value), respectively, were under-run clearly, also concerning the individual sampling days. 81 % and 92 % of the samples of the lake Werbeliner See (except the inflow site) were below the detection limit of 0,1 PFU . ml-1 for somatic and F+ RNA coliphages, respectively. 5. In contrast, DNA and RNA of all examined virus could be found in the lake. Quantitative PCR reveald a virus load between 0 und 105 Gen.equiv. . l-1 for Entero-, Noro- and Astroviruses. 6. The detection of infectious Enteroviruses on BGM cell lines yielded one positve sample (=1,2%). Since the concentration of Enterovirus genomes in the quantitative PCR was three to four log-steps lower then this of the Noro- and Astroviruses, one could specualte, that the concentration of infectious Noro- and Astroviruses in the lake was quite high. 7. The low rate of positive infectious Enteroviruses in the lake reflects the low concentration of bacteriophages. But the direct comparison of the concentration of bacteriophages with the concentration of infectious Enteroviruses in the flooding water casts doubt on the indicatior value of bacteriophages regarding Enteroviruses. In conclusion, this finding suggests that an exclusive indication of bacteriophages concerning a contamination with Enteroviruses is not possible. 8. The use of bacteriophages as indicators of a wider range of enteric viruses could attenuate this discrepancy by the epidemiological more frequent appearance of enteric viruses as a sum. 9. In this context bacteriophages could, due to their comparatively high resistence, have a key function in the assesment of the hygienic quality of a water sample. / Problemstellung: Um die hygienische Qualität von Badegewässern zu beurteilen existieren nationale und übernationale Richtlinien (EU-Badegewässerrichtlinie, WHO-Richtlinien). E.coli, Intestinale Enterokokken und Enteroviren dienen dabei als Indikatoren für die Belastung des Gewässers mit humanpathogenen Keimen bzw. Viren. Als Indikatoren für die virale Belastung sind in diesem Zusammenhang unter anderem Bakteriophagen im Gespräch. Gegenstand dieser Arbeit sind die Evaluierung von Somatischen und F+ RNA Coliphagen als Indikatoren für die Kontamination mit enteralen Viren am Beispiel eines Tagebausee sowie die Dokumentation der hygienischen Wasserqualität des Tagebausees unter Berücksichtigung des Flutungswassers. Vorgehensweise: In wöchentlichen Routineuntersuchungen wurden die Konzentrationen bakterieller und viraler Indikatoren, sowie die An- oder Abwesenheit enteraler Viren im Tagebausee &amp;quot;Werbeliner See&amp;quot; untersucht. Verschiedene, hygienische relevante enterale Viren (Entero-, Noro-, Astro-, Adeno-, Rotaviren, sowie Hepatitis A-Viren) wurden mittels geeigneter molekularbiologischer und zellkultureller Methoden detektiert und quantifiziert. Ergebnisse: 1. Die Kläranlage Leipzig-Rosental eliminierte sehr effizient (ca. zwei Größenordnungen) fäkale Bakterien und Bakteriophagen, wobei die Reduktion von E.coli und Enterokokken signifikant höher war als die von Cl.perfringens-Sporen und Bakteriophagen. Die Reinigungsleistung wird durch den Einsatz von chemischen Fällmitteln (Eisen-(III)-Chlorid / -Sulfat-Salze) begünstigt. 2. Noro-, Astro- und Enteroviren, für die quantitative Untersuchungen durchgeführt worden, konnten durch die Kläranlage um ein bis zwei Größenordnungen reduziert werden. 3. Alle untersuchten Viren konnten in Zu- und Ablauf der Kläranlage molekularbiologisch nachgewiesen werden. 4. Die Konzentration fäkaler Bakterien im Werbeliner See war sehr gering. Die Vorgaben der EU-Badegewässerrichtlinie (76/160/EWG) von 2000 . (100 ml)-1 (E.coli, Grenzwert) bzw. 100 . (100 ml)-1 (Enterokokken, Leitwert) wurden dabei, auch bezogen auf die einzelnen Probenahmetage, deutlich unterschritten. 81 % bzw. 92 % der Seeproben (außer Einleitungsstelle) lagen unterhalb der Nachweisgrenze von 0,1 PFU . ml-1 für Somatische- bzw. F+ RNA Coliphagen. 5. Im Kontrast dazu konnte DNA bzw. RNA aller untersuchten Viren im See gefunden werden. Die genomische Viruslast der mittels real-time-PCR detektierten Entero-, Noro- und Astroviren lag zwischen 0 und 105 Gen.äquiv. . l-1. 6. Der Nachweis infektiöser Enteroviren auf BGM-Zellen im Werbeliner See ergab ein einzelnes positives Ergebnis im Untersuchungszeitraum (= 1,2 %). Allerdings lag die Konzentration detektierter Enteroviren-Genome im Mittel drei bis vier Größenordnungen unter der Konzentration von Noro- und Astroviren. Es kann deshalb spekuliert werden, dass für diese Viren ebenfalls ein hoher Anteil infektiöser Proben zu erwarten gewesen wäre. 7. Die niedrige Nachweisrate infektiöser Enteroviren im Werbeliner See spiegelte sich in der sehr niedrigen Konzentration an Bakteriophagen wider. Allerdings lässt der direkte Vergleich der Bakteriophagenkonzentration mit dem Nachweis infektiöser Enteroviren im Flutungswasser Zweifel am Indikatorwert von Bakteriophagen für eine Kontamination mit Enteroviren aufkommen. Eine exklusive Indikation von Bakteriophagen bezüglich einer Kontamination mit Enteroviren scheint anhand dieser Befunde nicht möglich. 8. E.coli und Enterokokken wurden in Laborversuchen unter verschiedenen Versuchsbedingungen signifikant schneller inaktiviert als Somatische Coliphagen und Noroviren. Gleiches galt im Vergleich zu Cl. perfringens-Sporen, Astroviren und F+ RNA Coliphagen. 9. Bakteriophagen könnten deshalb auf Grund ihrer vergleichsweise hohen Resistenz eine wichtige Schlüsselfunktion für die Beurteilung der hygienischen Qualität einer Wasserprobe spielen.