<p>Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today. </p><p>This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. Proximity ligation converts information about the presence or co-localization of specific proteins to unique sets of nucleic acid sequences. For detection of target proteins or protein complexes the coincident binding by pairs or triplets of specific protein-binding reagents are required. Oligonucleotide-extensions attached to those binding reagents are joined by a DNA ligase and subsequently analyzed by standard molecular genetic techniques. The technique is shown to sensitively detect an assortment of proteins using different types of binders converted to proximity probes, including SELEX aptamers and mono- and polyclonal antibodies. I discuss factors important for using the technique to analyze many proteins simultaneously.</p><p>Quantification of target molecules requires precise amplification and detection. I show how rolling circle amplification, RCA, can be used for precise quantification of circular templates using modified molecular beacons with real-time detection. The combination of proximity-probe templated circularization and RCA results in a sensitive method with high selectivity, capable of visualizing individual immobilized proteins. This technique is used for localized detection of a set of individual proteins and protein complexes at sub-cellular resolution.</p>

Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today. This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. Proximity ligation converts information about the presence or co-localization of specific proteins to unique sets of nucleic acid sequences. For detection of target proteins or protein complexes the coincident binding by pairs or triplets of specific protein-binding reagents are required. Oligonucleotide-extensions attached to those binding reagents are joined by a DNA ligase and subsequently analyzed by standard molecular genetic techniques. The technique is shown to sensitively detect an assortment of proteins using different types of binders converted to proximity probes, including SELEX aptamers and mono- and polyclonal antibodies. I discuss factors important for using the technique to analyze many proteins simultaneously. Quantification of target molecules requires precise amplification and detection. I show how rolling circle amplification, RCA, can be used for precise quantification of circular templates using modified molecular beacons with real-time detection. The combination of proximity-probe templated circularization and RCA results in a sensitive method with high selectivity, capable of visualizing individual immobilized proteins. This technique is used for localized detection of a set of individual proteins and protein complexes at sub-cellular resolution.

The RNA polymerase T7 is utilized as a component of motor complexes in DNA nanotechnology due to its high promotor specificity, the lack of external transcription factors and its very high processivity, but there is no experience of its application on small double stranded DNA circles. Circular templates from 210 to 126 bp in circumference sharing a common promotor termination motif were synthesized and transcription was monitored at end point on gel and in real time with a 2’ O methyl RNA molecular beacon. The RNAP T7 was found to be able to utilize circular dsDNA templates down to 126 bp with moderate impact on transcription rate for saturated systems and rolling circle transcription products were evident with denaturizing PAGE gel electrophoresis for templates down to 167 bp.

The goal of the thesis is to design the star-based mesh topology by introducing multiple pan-coordinators (hub/switches) under a multipath-fading environment and to improve the data transaction rate of a network which usually gets worst when there is a single pan-coordinator for synchronization of devices in conventional mesh topology; also reduce the hop-count as least as possible. Most of the work has been done on NS-2 network simulator; therefore the research model which has been used here is a simulation model. Altogether 3 simulations have been done. The first scenario is done on a simplest mesh network with a single coordinator and a radio propagation model which has been used is two-ray ground reflection model. The second scenario simulation is similar to the first scenario but in-order to provide multi-path signal fading and highly congested environment the propagation model which has been used this time is shadowing model. The final simulation which has been done is of multiple-star based mesh topology it also uses the similar radio propagation model which has been defined for second scenario. An intensive performance measurement of all the three simulations has been done in terms of transactions made per-second, packet drop rate along with an analysis of packet drop. An hop-count is also measured between star and mesh topology. For multiple star based mesh topology it can be assumed if multiple stars with a routing capability can be used then nodes in a network will be synchronized or re-synchronized with least number of hops in the congested network with a near-by pan-coordinator (hub/switch). One of the major applications of this topology can be automobile manufacturing industry where alot of machines are installed in a congested network and monitoring of every area is mandatory for swift production.

The Mobile Electronic Dispensary System (MEDS) is an indoor medical dispensary system where robots locate and travel to patients within a grid in order to deliver medication or other medical supplies based on a predefined schedule. For older people or individuals with physical or mental disabilities, it is important to ensure that medications are taken as prescribed. Missing or mixing dosages can cause unwanted and even harmful consequences. As individuals grow older or battle disabilities, it is expected that adhering to their medicine regimen will be a daily challenge without the assistance of a fulltime caregiver. Therefore, to assist individuals in maintaining their independence, MEDS ensures the proper medicine is dispensed to the patient at the prescribed time and dosage. At the core of MEDS is a scheduler that maintains the medicines to be dispensed, including the times and dosages. Once a scheduled time arrives to deliver medicine to a patient, MEDS instructs the appropriate robot to wake up, locate the patient within a defined grid, and then travel to the patient and deliver the medicine. Upon receiving the delivery, the patient will accept the medicine physically and then update their mobile device, informing MEDS that the medicine was successfully delivered. At this time, the robot will return to its home base within the grid. The patients are within the confines of a building where GPS is not a viable solution to track items to pinpoint accuracy. Therefore, an indoor location based system with beacons and listeners are required in order to define a grid and enable robots to locate and travel to the patient. This paper defines and details the programs, database, algorithms, and hardware of MEDS using the Cricket Indoor Location System and iRobot Creates. / text

Since digital media entered the business domain, many different tools and platforms have transformed the nature of business communications. This transformation has not been easy, since the journey has been accompanied by challenges from the marketers’ side against the adoption of the new platforms into the firm’s communication channels. Business to business marketers have also been engaged in such challenges by maintaining a slow adoption, which has motivated the researchers to study the adoption of different means and tools of digital communication in a business context. Through this research, I contribute by exploring the adoption strategies of digital platforms in the B2B supply chain including B2B firms, retailers and end users. By digital media, I mainly refer to social media and beacon technology. First, I begin this thesis by identifying the adoption and integration strategies of social media and digital marketing into traditional marketing channels in a B2B context. In this part, I identify the B2B firms’ target audience and propose a model facilitating a B2B firm’s practical social media adoption strategies. Second, to explore the benefits of different social media content sharing approaches derived from information accessibility resulted in the prior study of this thesis, I introduce and examine Public and Gated-Content sharing approaches. Thereafter, assisted by Social Power Theory and Resource Dependence Theory, I examine the effect of Public and Gated-Content sharing approaches on the target audience’s willingness to interact with the firm. The findings of this study reveal that Gated-Content approach can help the firms build closer relationship with the target audience and engage them in a co-creation process. Third, by studying proximity marketing through the adoption of beacon technology in the retail context, I explore the current methods of usage, as well as the benefits and challenges of in-store proximity marketing adoption for content sharing purposes. I complete the thesis by presenting the different challenges of such adoption, which consist technical, human behavior, managerial perception, resource and privacy factors. Finally, I identify the need to integrate the physical aspect of place and location back again into the online digital communication channels within a retail context. / <p>QC 20171219</p>

This thesis deals with design of navigation system for autonomous mobile robots, which is based on the infrared light. The system is based on measuring the relavive angles using landmarks in the enviroment that make the robot can orient and recognize its absolute position in an environment in which it operates.

No description available.

Lost&Found Videothek is the concept for an interactive video library thought for users engaged in an exploratory research mode, in which content is organized according to a collaborative, subjective and implicit indexing system for the enhancement of serendipity. This idea was the result of a design-based research with a user-centered approach in the field of information retrieval. With the use of design methods such as heuristic UX evaluation, co-sketching, and other original design experiments, the project aimed at identifying the most valuable qualities of physical and digital libraries in order to integrate them within one retrieval system.

With the coming growth in Internet of Things (IoT) applications, we can expect environ-ments with many independent networks operating in nearby locations. Wireless Sensor Networks (WSN), which have become popular during the last few years, are the main type of networks used in IoT. The IEEE 802.15.4 protocol designed for low-rate wireless personal area networks has been widely adopted for this kind of network. Together with ZigBee, this protocol is gaining increasing interest from the industry, as they are con-sidered a universal solution for low-cost, low-power, wireless connected monitoring and control devices. Internetwork interference issues in IEEE 802.15.4 networks can be a ma-jor problem because of the extensive use of wireless channels. In this thesis, an in-depth simulation study of the internetwork interferences is performed using Castalia, a widely used network simulator. We focus on the beacon collision problem, as it has been proved to be the main cause of performance degradation for coexisting networks. We carry out a prestudy of the main node simulation parameters to setup the different scenarios. Then, we evaluate how the overlap of the active periods and the location of the nodes affect the network performance. We continue with a network coexistence analysis to study the inter-action of two networks of two nodes and their performance regarding the beacon reception rate. We show that there are significantly different operation regions, depending on the network location. Following this, a probabilistic analysis is carried out in order to obtain an average beacon reception rate depending on the size of the area considered. Finally, we discuss available beacon collisions avoidance methods, taking into account the detailed simulation results. Our conclusions have theoretical and practical implications for the design of wireless sensor networks, and for the evaluation of beacon collisions avoidance schemes. / Med den kommande tillväxten i sakernas Internet (IoT) applikationer är miljöer med många oberoende nätverk som verkar på närliggande ställen väntat. Trådlösa sensornätverk (WSN), som har blivit populära under de senaste åren, är den vanligaste typen av nät som används i sakernas Internet. IEEE 802.15.4 protokollen, konstruerad för låghastighet trådlösa personlig area nätverk, har fått stor spridning för WSNs. Tillsammans med ZigBee, får de en snabb ökat intresse från industrin, eftersom de betraktas som en universallösning för låg kostnad låg energi trådlös anslutning för övervaknings- och kontrollinstrument. Internätverk interferens i IEEE 802.15.4 nätverk kan vara ett stort problem på grund av den omfattande användningen av trådlösa kanaler. I denna avhandling är en djupgående simulation studie utfört med hjälp av Castalia, ett utbrett använt nätverk simulator. Vi fokuserar på beacon kollisionsproblem, eftersom det har visat sig vara den främsta orsaken till prestandaförsämring för samexisterande nätverk. Vi utför en förstudie av den viktigaste simulation parametrarna för att bestemma de olika scenarierna. Sedan utvärderar vi hur överlappningen av de aktiva perioderna och placeringen av noderna påverkar nätverkets prestanda. Vi fortsätter med en nätverksamexistens analys för att studera interaktionen mellan två nätverk av två noder, och deras prestanda avseende beacon mottagnings kvot. Vi visar att det finns betydligt olika operationsområdena, beroende på nätverksens placering. Därefter är en sannolikhetsanalys utfört för att erhålla en genomsnittlig beacon mottagnings kvot, beroende på storleken på betraktade områden. Slutligen diskuterar vi tillgängliga metoder för att undvika beacon kollision, med hänsyn till de detaljerade simuleringsresultaten. Våra slutsatser har teoretiska och praktiska kon-sekvenser för utformningen av trådlösa sensornätverk, och för utvärderingen a metoder för att undvika beacon kollision.