In agreement with global patterns of climate change and increasing temperatures in the tropical oceans, the Northern Red Sea (NRS) has been warming over the last few decades. Using 18 years of remotely-sensed chlorophyll-a data (Chl-a, an index of phytoplankton biomass), we investigate the potential impacts of climate warming on phytoplankton abundance and phenology in the Northern Red Sea by exploring the mechanistic links with the regional physical environment. The results of the analysis reveal that, in accordance with other tropical ecosystems, phytoplankton biomass in the NRS will decrease in response to warmer climate scenarios. This is attributed to lower heat fluxes (heat loss to the atmosphere) during the bloom period, and enhanced vertical stratification, which prevents vertical mixing of nutrients into the euphotic layer. In addition, we show that during warmer conditions (when heat fluxes are weakened), the winter bloom initiates significantly later (by up to 10 weeks) and its duration is considerably reduced. The biological implications of alterations to phytoplankton phenology may include increased larval mortality of pelagic species, reduced recruitment, fisheries impacts and changes to community structure.

From the compelling need of school to become more attractive to the students, it seeks to stimulate the practices that approach the school contents to the reality, as an alternative to innovate the learning. From the premise that the teaching of algorithm in high school in the traditional way is a hard task in schools, the present work aims to verify the efficiency of the insertion of arduino hardware prototyping platform as Information and Communication Technology to support the process of teaching and learning in the classroom. This work, proposed in the Postgraduate Program in Educational Technologies in Network, in particular in the research line of Development of Educational Technologies in Network, suggests the insertion in traditional methods of teaching of this programmable platform of hardware capable of becoming a support, school help, complementation of class, in order to involve the student, through the exploration of his or her curiosity, associated to the contact and handling of the equipment, in the longing that it substitutes the role of spectator by the leading role in the process of teaching and learning. The educational product described in this work is about five objects of learning constructed from the Arduino hardware, to assist in the comprehension of covered contents in the subject of Programming I in the Technical Computing Course in the campus of IF Farroupilha in Santo Augusto. Each one of the OL (objects of learning) was built to the light of Bloom Taxonomy, which standardizes the language in which the objectives of learning are presented. The other methods used are the ones of qualitative approach and case study. Each object was presented to the students in different pedagogical moments in order to, from each experiment, present one of the contents that introduces the teaching of algorithms. The instruments to conduct the search were based on ethnographic techniques of audio and video recording, besides textual summary, where there was the pedagogical intervention of the researcher, presenting the experiments to the students, which were distributed in pairs or trios. The systemization of data was performed observing the Discursive Textual Analysis, having in mind the need of comprehension of the process. It was possible to realize that the use of experiments in the classroom aroused the interest in the students, in addition to a bigger degree of compromise in the attempt of the students to comply with what was proposed and understand what was happening in each situation problem. The experiments served as a support tool in the learning because the students engaged themselves in the task of discovering in group the correct words to comprehend the meaning of each experiment and systematize it through a textual description and subsequently a pseudocode. It is believed that because of the popularity of Arduino, facility of construction of experiments and because of the fact of being a free technology it is possible to characterize the objects of learning as a viable alternative as a didactic resource to the teaching of algorithms, taking advantage of its potential in public schools, usually lacking in resources. / A partir da necessidade premente da escola tornar-se mais atrativa aos alunos, busca-se estimular as práticas que aproximam os conteúdos escolares da realidade, como alternativa para inovar a aprendizagem. Partindo da premissa de que o ensino de algoritmos no nível médio da maneira tradicional é tarefa árdua nas escolas, o presente trabalho tem como objetivo verificar a eficiência da inserção da plataforma de prototipação de hardware Arduino como Tecnologia de Informação e Comunicação para apoio ao processo de ensino e aprendizagem em sala de aula. Este trabalho, proposto no Programa de Pós Graduação em Tecnologias Educacionais em Rede, mais especificamente na linha de pesquisa Desenvolvimento de Tecnologias Educacionais em Rede, sugere a inserção nos métodos tradicionais de ensino desta plataforma de hardware programável capaz de tornar-se um suporte, reforço ou complementação da aula, para que envolva o aluno, através da exploração de sua curiosidade, aliada ao contato e manuseio do equipamento, no anseio que substitua o papel de expectador pelo de protagonista do processo de ensino e aprendizagem. O produto educacional descrito neste trabalho trata-se de cinco objetos de aprendizagem construídos a partir da ferramenta Arduino, para auxiliar na compreensão de conteúdos abordados na disciplina de Programação I do curso Técnico em Informática no Campus do IF Farroupilha de Santo Augusto. Cada um dos OA foram construídos à luz da Taxonomia de Bloom, que padroniza a linguagem em que os objetivos da aprendizagem são apresentados. Os demais métodos utilizados são o de abordagem qualitativa e estudo de caso. Cada objeto foi apresentado aos alunos, em diferentes momentos pedagógicos, com o intuito de, a partir de cada experimento, apresentar um dos conteúdos que introduz o ensino de algoritmos. Os instrumentos para realização da pesquisa foram baseados em técnicas etnográficas de gravação de áudio e vídeo, além de resumo textual, onde houve a intervenção pedagógica do pesquisador, apresentando os experimentos para os alunos, que estavam distribuídos em duplas ou trios. A sistematização dos dados foi realizada observando a Análise Textual Discursiva, tendo em vista a necessidade de compreender o processo. Foi possível perceber que o uso dos experimentos em sala de aula despertou interesse nos alunos, além de um grau de comprometimento maior na tentativa dos alunos de cumprir o que foi proposto e entender o que se passava em cada situação problema. Os experimentos serviram como ferramenta de auxílio na aprendizagem, pois os alunos empenharam-se na tarefa de descobrir em grupo as palavras corretas para compreender o significado de cada experimento e sistematizá-lo através de uma descrição textual e posteriormente um pseudocódigo. Acredita-se que pela popularidade do Arduino, facilidade de construção dos experimentos e pelo fato de ser uma tecnologia livre, é possível caracterizar os objetos de aprendizagem como uma alternativa viável como recurso didático para o ensino de algoritmos, aproveitando seu potencial em escolas públicas, usualmente carente de recursos.

La métagénomique vise à étudier le contenu génétique et génomique d'un échantillon provenant d'un environnement naturel. Cette discipline récente s'attache à étudier les génomes de différents organismes provenant d'un même milieu. La métagénomique pose de nouvelles questions, tant d'un point de vue biologique qu'informatique. Les masses de données générées par les études métagénomiques et la complexité des milieux étudiés, nécessitent de développer de nouvelles structures de données et de nouveaux algorithmes dédiés. Parmi les différentes approches existantes en métagénomique, la métagénomique comparative consiste à comparer plusieurs métagénomes afin d'en connaître les divers degrés de similarité. Lorsque cette comparaison se base uniquement sur le contenu brut des échantillons, sans faire appel à des connaissances externes, on parle de métagénomique comparative de novo. L'objectif des travaux que nous proposons est de développer une méthode permettant d'extraire les séquences similaires de deux jeux de données métagénomiques, où chaque jeu peut être composé de centaines de millions de courtes séquences. La comparaison proposée consiste à identifier les séquences d'un premier jeu similaires à au moins une séquence d'un second jeu. Afin d'être rapide et économe en mémoire, l'implémentation de notre méthode a nécessité la conception d'une nouvelle structure d'indexation, basée sur le filtre de bloom. Le logiciel final, nommé Compareads, a une consommation mémoire faible (de l'ordre de quelques go) et peut calculer l'intersection de deux échantillons de 100 millions de séquences chacun en une dizaine d'heures. Notre méthode est une heuristique qui génère un faible taux de faux positifs. Le logiciel Compareads est dédié à l'analyse de grands jeux de données métagénomiques. À l'heure actuelle, il est le seul outil capable de comparer de tels jeux. Compareads a été appliqué sur plusieurs projets métagénomiques. Notre outil produit des résultats robustes, biologiquement exploitables et en accord avec diverses méthodes fondamentalement différentes. Il est actuellement utilisé de manière intensive sur les échantillons provenant de l'expédition tara oceans. Sur ce projet, notre méthode à permis de mettre en évidence que les grands systèmes océaniques influent sur la répartition globale des micro-organismes marins. / Metagenomics studies overall genomic information of multiple organisms coming from the same biotope. The information is generally provided by next generation sequencing technologies (NGS). Typical data are samples of short reads (i.e. reads of few hundred base pairs). To study such metagenomics information, we developed an original method for extracting similarities between two samples of reads. More precisely, this approach locates the set of common reads present in two samples. In order to fit with current memory capacities and to be time efficient, we used a modified Bloom filter data structure. Finding the common reads between multiple samples and crossing this information with the location of samples leads to visualize some biological processes like ubiquitous species or effect of water stream caring some species. Finally, the tool can also be used as a filter on metagenomics datas to remove for example only one specie. Our software, Compareads, is actually used on the Tara Oceans project where it shows that global dynamic of oceans seems to play a part on the dispersion of marine microorganisms.

No description available.

No description available.

Recent research in microbial ecology has focused on how aquatic bacterial communities are assembled. Only a few of these studies follow a “Gleasonian” approach where the roles of single bacterial populations are in focus. In this thesis, novel molecular tools were used to describe the distribution and evolutionary relationships of microbes in productive aquatic environments. Many new phylogenetic groups of bacteria were identified, likely representing bacterial populations restricted to productive freshwaters. I also addressed the dynamics and functional role of individual bacterial populations in eutrophic lakes and brackish environments with a focus on either biogeochemically significant or potentially pathogenic representatives. Flavobacteria blooms were observed, on occasions characterized by high heterotrophic production. In addition to high temporal dynamics microbial community composition and function differed on the spatial scale, as exemplified by free-living and Cyanobacteria-associated habitats. At the community scale, microbial processes, such as biomass production and substrate uptake could be predicted from the presence and absence of individual bacterial populations. I also studied the niches of potentially pathogenic Vibrio populations in various coastal waters. Using a novel culture-independent method, a V. cholerae population was detected along the entire Swedish coastline. Results from an environmental survey and a laboratory mesocosm experiment reveal that phytoplankton-derived dissolved organic matter enhance the growth of V. cholerae and other Vibrio spp. and hence create a largely overlooked niche for these heterotrophic bacteria. This thesis and future work on the role of individual bacterial populations will facilitate predictions of biogeochemical cycles and the distribution of bacteria in the context of global climate change and local eutrophication.

Gosse’s reputation, both during his lifetime and thereafter, was compromised by his propensity for error, a trait that Henry James famously described as ‘a genius for inaccuracy’. Though much of his biographical and critical writing justifies this criticism, my study of Gosse’s use of the device of allusion, mainly in his fictional writing, reveals a strategy of misprision that is creative and innovative. Since the concepts of Modernism and Postmodernism have changed the way in which texts are read, it is now time to re-read Gosse, and to explore the potential meaning of passages that would hitherto have been dismissed as error or exaggeration. Using Ziva Ben-Porat’s characterisation of allusion ‘as a device for the simultaneous activation of two texts’ as my methodology, I explore the complex and often subversive resonances of Gosse’s allusive practice. Allusion requires four participants: author, reader, the source text by the precursor, and the alluding text. Because a phrase does not ‘become’ an allusion until all four parties have been ‘activated’, many of Gosse’s allusions have for a long time lain dormant in the palimpsest of his writings. I argue that Gosse’s evangelical, tract-writing mother, rather than his father, exerted primary influence on him. I foreground the impact of her prohibition of fiction as the genesis for Gosse’s idiosyncratic vision, showing that its legacy was more bewildering, and ironically more creative, than has hitherto been recognised. Using the revisionary ratios of Bloom’s theory of the anxiety of influence, I establish a trajectory of charged interactions between the texts of Gosse as ephebe and those of his mother as precursor. Many hitherto puzzling and unresolved aspects of Gosse’s writing now make sense in the context of his ‘answering back’ the spectral Bowes. Although Gosse never fully extricates himself from his maternal precursor, he metaphorically orphans himself, and transfers his ephebe allegiance to a host of literary fosterfathers, constantly invoking them in his texts. He thus secures his ‘mental space’ through the covert mode of allusion, and the zenith of this practice is manifested in Father and Son. My thesis demonstrates the potential of allusion as a methodological tool in literary analysis. By his acts of re-reading, Gosse achieves the paradoxical act of simultaneously arresting and promoting a sense of cultural continuity. On the one hand, Gosse arrests tradition by fragmenting texts: by importing a phrase or a passage from a past work into his present text, he engenders textual instability in both. On the other hand, Gosse promotes cultural continuity by importing into his work fragments that serve as allusive bridges forging connections through space and time. I hope that this exploration of his practice will initiate a reassessment of Gosse’s role in relation to the allusive mode as employed by the early Modernists.

Few data are available on distribution, abundance, and ecology of scyphozoans in the Northern California Current (NCC). This thesis is divided into four chapters, each of which contributes to our understanding of a different stage of the scyphozoan life history. The first study describes the settlement preferences of Chrysaora fuscescens planulae in the laboratory. Planulae were found to respond to the interaction of substrate and orientation. Artificial substrates were identified as viable habitat for C. fuscescens. In the second chapter, a population of scyphistomae in Charleston, Oregon were identified to species-level using DNA barcoding techniques. The third and fourth chapters focus on the medusa stage of the life history. Using surveys mailed to fishers in the Pacific Region, this study provides baseline data on the types and magnitudes of economic damages caused by jellyfish on different fisheries and helps assess fishers' perceptions of jellyfish population trends in the NCC. This thesis includes previously unpublished co-authored material.

Eutrophication is a global problem affecting many inland and estuarine waters. Many wetlands on the Swan Coast Plain, in Western Australia, have undergone increasing nutrient enrichment since European settlement of the region in the 1850’s. Problems such as algal blooms and nuisance swarms of non-biting midges (Diptera; Chironomidae) are the consequence of nutrient enrichment in many of these wetlands. The restoration of these degraded wetlands, especially with respect to reducing nutrient enrichment, requires a range of comprehensive and effective techniques including catchment management, diversion or treatment of surface inputs and treatment of enriched sediments. Nitrogen and phosphorus, especially phosphorus, are not the only factors controlling algal biomass in water bodies, but they are the only elements that can be removed efficiently and economically. Internal P cycling from wetland sediments can initiate and sustain eutrophication and related algal blooms and nuisance midge problems even after external sources are diverted or reduced. The aim of this study was to identify an effective material to reduce sediment phosphorus release and thereby the phosphorus concentration of the water column. It was also important to determine the impact of the selected amendment material on phytoplankton and larval midge (chironomid) communities. A range of experiments at increasing scales, from bench-top, to microcosm to outdoor mesocosm experiments were designed to test three hypotheses: 1) Materials which have a high P sorption capacity, over a wide range of P solution concentrations, and low P release rate, are potentially suitable agents to reduce P in wetlands with enriched sediments by inactivating sediment P; 2) A reduction in the abundance of cyanobacteria caused by increasing the N:P ratio of an aquatic ecosystem results in a reduction in the density of nuisance species of Chironomidae. 3) Successful amendment of enriched sediments reduces P in the water column thereby reducing the total phytoplankton biomass and the related density of nuisance species of Chironomidae. The adsorption and desorption experiments were carried out under a range of pH values and P concentrations, with a number of materials including fly ash, red mud, precipitated calcium carbonate, crushed limestone and lime to determine the maximum adsorption capacity and affinity of these materials. A rang of P concentrations (0-1000 µg/L) simulated the P concentration of the water column in a range of wetlands of differing trophic status. Poor fits to the Langmuir equation occurred with both red mud and fly ash due to their high P content. A good fit occurred with lime, with a high P removal rate (90%-96%) over the same range. Fly ash and red mud were eliminated from further investigation due to the possibility that they might release phosphorus rather than absorb when P concentrations in surrounding environment were less than 300 µg/L or 200 µg/L respectively (concentrations which can occur in eutrophic systems). Among the three lime-based, redox-insensitive materials tested in the second mesocosm experiments, precipitated calcium carbonate (PCC) possessed the highest maximum adsorption capacity and lowest desorption rate under a range of pH values (6.2, 7.2 and 10) and P concentrations (0-12 000 µg/L), followed by crushed limestone and lime. The different maximum absorption capacities of the three materials appears to be mainly attributed to their particle size (surface area). Lime was chosen as the amendment material for further investigation because it was the only one of the three available in sufficient quantities within the timeframe of this study. Microcosm experiments showed that lime was effective in reducing sediment P release from intact sediment cores, and the ratio of TN:TP in the treatment cores increased over time compared to the control cores (in which TN: TP decreased slightly). In the first mesocosm experiment a significantly higher density of larval midges was found in the treatments than in the controls. The treatments were aimed to increase N:P ratio in the systems to reduce cyanobacteria and, subsequently, larval midge densities. However even though cyanobacteria were eliminated from the treatments, the nitrogen addition appeared to result in higher phytoplankton biomass overall, which fuelled an increase in larval midge densities. In the second mesocosm experiment, the addition of lime to enriched sediments resulted in a reduction in P in the water column. This reduction was accompanied by a reduction in total phytoplankton biomass, the absence of cyanobacteria, and a less abundant and more species - diverse chironomid fauna in the treatment mesocosms. Sediment P fractionation undertaken for both the microcosm and mesocosm experiments showed that most of the phosphorus adsorbed by lime was in the labile fraction (NH3Cl extractable P and NaOH extractable P). Phosphorus in the HCl extractable fraction was also found to be higher in the treatments due to the presence of inert mineral P in the lime than the formation of new hydroxyapatite from adsorbed P. The two mesocosm experiments suggested that larval midges were non-selective feeders, responding to total phytoplankton biomass, rather than the presence of cyanobacteria. Dissolved oxygen and predation also influenced larval midge densities. In summary, although lime appeared to be a useful material for reducing P release from enriched sediments under controlled laboratory conditions, the effect under field conditions was not as definitive. Further work is required to more fully determine the conditions under which sediment remediation may be used as a means of controlling sediment P release and associated high densities of larval chironomids.

<p>Att skapa rättvisa prov är något av det svåraste som finns för alla lärare. Denna rapport redogör för ett undervisningsförsök i att praktiskt använda Bloom´s reviderade taxonomi för att validera frågorna till ett prov, som byggs upp för och genomförs på dator. Undersöknings-gruppen utgörs av mina elever som läser kursen programmering A på gymnasienivå. En norsk undersökning har tidigare visat att pojkars provresultat höjs om proven utförs på dator, vilket också visade sig bli resultatet i min undersökning. Men till skillnad från den norska undersökningen så ökade också flickornas resultat i min undersökning. Med hjälp av denna teknik att skapa prov, skulle det vara enkelt och möjligt att skapa nationella datorbaserade prov inom flera olika kurser som skulle kunna vara till hjälp för lärare ute i landet att hitta rätt kunskapsnivå på kurserna.</p>