Captive husbandry programs in zoos have documented nesting behavior and have successfully hatched Manouria emys emys, but data on sex determining mechanisms and sex ratios are absent. A total of 30 M. e. emys eggs were artificially incubated at five different temperatures in constant humidity. Mean incubator temperatures were 24.99°C, 25.06°C, 27.18°C, 28.00°C, and 30.79°C. Incubation duration ranged from 60 days to 92 days, and hatching success was 50%. Sex determined by histology and laparoscopy resulted in male differentiation at low temperatures (24.99°C, 27.18°C) and female differentiation at high temperatures (30.79°C). Pivotal temperature was estimated to be 29.29°C. The following investigation into temperature-dependent sex determination (TSD), including its presence or absence, pattern, and pivotal temperature, has implications for studies of adaptive significance of reproductive behaviors and of chelonian phylogenetic history. Additionally, the proposed study can provide foundations for conservation management decisions, and for captive breeding programs.

This dissertation examines American attitudes towards death from the colonial era to the end of the nineteenth century. I begin with a close analysis of the thanatology of the Congregational church in New England, before demonstrating the lasting influence of Puritan thought on three later writers: Jonathan Edwards, Henry David Thoreau and Stephen Crane. In contrast to purely cultural studies of mortality in America (including those by Phillipe Ariès, David Stannard and Michael Steiner), my investigation discusses the philosophical difficulties that obstruct any attempt to speak about death. Building on Jacques Derrida’s work in Aporias (1993), I identify three logical impasses that interrupt Puritan writing on mortality: the indeterminacy, singularity and finality of death. While Edwards, Thoreau and Crane write in different circumstances and diverse genres, I argue that they are sensitive to these same three aporias when they discuss death. In this regard, they resist a broader post-Puritan tendency (in both scientific and sentimental texts) to minimize the uncertainties surrounding human mortality and approach death as a universal (rather than radically singular) phenomenon. While my study situates each of its authors in the cultural and intellectual contexts in which they worked, it also challenges the notion that it is possible to write a history of death. Speaking strictly, mankind’s relationship to death can never change. It is always, in fact, a non-relation. The very idea of death destabilizes our most fundamental historical and literary assumptions. Accordingly, my second chapter uses a deconstruction of Edwards’ theory of revivalism to argue that the New-England awakenings of the eighteenth century expressed the converts’ desire to renounce responsibility for their souls, rather than accept it. In my third chapter, I argue that those writings in which Thoreau registers what might seem to be a nihilistic fascination with dead and decaying bodies in fact express a sentimental desire for a peaceful death. Chapter four reads Stephen Crane’s poetry, fiction and journalism in the context of his Calvinist heritage, breaking down the distinction between his textual play with the concept of death and the Puritans’ “serious” attempts to come to terms with mortality.

This dissertation examines American attitudes towards death from the colonial era to the end of the nineteenth century. I begin with a close analysis of the thanatology of the Congregational church in New England, before demonstrating the lasting influence of Puritan thought on three later writers: Jonathan Edwards, Henry David Thoreau and Stephen Crane. In contrast to purely cultural studies of mortality in America (including those by Phillipe Ariès, David Stannard and Michael Steiner), my investigation discusses the philosophical difficulties that obstruct any attempt to speak about death. Building on Jacques Derrida’s work in Aporias (1993), I identify three logical impasses that interrupt Puritan writing on mortality: the indeterminacy, singularity and finality of death. While Edwards, Thoreau and Crane write in different circumstances and diverse genres, I argue that they are sensitive to these same three aporias when they discuss death. In this regard, they resist a broader post-Puritan tendency (in both scientific and sentimental texts) to minimize the uncertainties surrounding human mortality and approach death as a universal (rather than radically singular) phenomenon. While my study situates each of its authors in the cultural and intellectual contexts in which they worked, it also challenges the notion that it is possible to write a history of death. Speaking strictly, mankind’s relationship to death can never change. It is always, in fact, a non-relation. The very idea of death destabilizes our most fundamental historical and literary assumptions. Accordingly, my second chapter uses a deconstruction of Edwards’ theory of revivalism to argue that the New-England awakenings of the eighteenth century expressed the converts’ desire to renounce responsibility for their souls, rather than accept it. In my third chapter, I argue that those writings in which Thoreau registers what might seem to be a nihilistic fascination with dead and decaying bodies in fact express a sentimental desire for a peaceful death. Chapter four reads Stephen Crane’s poetry, fiction and journalism in the context of his Calvinist heritage, breaking down the distinction between his textual play with the concept of death and the Puritans’ “serious” attempts to come to terms with mortality.

El objetivo principal de esta tesis se ha centrado en el estudio de las diferencias entre ratas macho y hembra en la morfología, la función y la biogénesis mitocondrial del tejido adiposo marrón (TAM) y del hígado, mediante el análisis de distintas subpoblaciones mitocondriales obtenidas a través del fraccionamiento de la población mitocondrial total. Los resultados han puesto de manifiesto que las diferencias entre géneros a nivel mitocondrial tanto en el TAM y como en el hígado podrían ser atribuidas a la existencia de una subpoblación mitocondrial altamente diferenciada en las hembras, hecho que podría ser indicativo de un proceso de biogénesis mitocondrial distinto entre ambos géneros. Los resultados sugieren la existencia de un factor común a ambos tejidos que influiría en la regulación de dicho proceso. En este sentido, las hormonas sexuales podrían ser uno de los factores candidatos responsables de las diferencias observadas en el presente trabajo. / The main goal of this thesis has been focused on the study of gender differences in the mitochondrial morphology, function and biogenesis both in brown adipose tissue (BAT) and in liver, through the analysis of the several mitochondrial subpopulations isolated by means of the fractionation of the whole mitochondrial population. Results have reflected that the gender dimorphism stated in mitochondrial population both in BAT and in liver could be attributed to the existence to more highly differentiated mitochondria in female rats, which could be the result of a different mitochondrial biogenesis process between genders. Since the existence of a common factor which influences this process in both tissues could be hypothesized, sexual hormones could be one of the main factors responsible for the differences described in the present work

En observant le contexte architectural actuel, on note que les architectes s'intéressent davantage à faire valoir l'apparence de la surface de l'enveloppe extérieure de leurs bâtiments. Ce déplacement nous interpelle sur les raisons derrière ce type de changement au niveau conceptuel en architecture. L'examen de deux ouvrages récents nous révèle l'existence d'une tension entre l'aspect significatif et l'aspect matériel, ou pour le dire autrement, entre le signe et la matière. Notre objectif sera de faire l’histoire de cette dialectique afin de mieux saisir la nature de ce déplacement et comment cette opposition a pu déplacer la conception vers la surface et vers l'enveloppe extérieure. Notre étude montre que l'hypothèse de base de la dialectique du signe et de la matière se divise en trois parties. La première expose la dialectique existante, dans les années 1950 en Angleterre, du formalisme de Colin Rowe et Robert Slutzky et d'autre part, de l'image des matériaux trouvés comme tels des brutalistes promus par Reyner Banham. En deuxième partie, cette dialectique se développe aux États-Unis à partir des armées 1960 pour prendre une autre forme d'opposition entre le modèle sémiologique de Robert Venturi et de Denise Scott Brown et en contre partie le projet tectonique de Frampton basé sur son interprétation matériel et significatif de la construction. L'opposition actuelle entre les descriptions phénoménologiques de la surface et l'ambition d'une politicisation significative de l'enveloppe constitue le troisième moment de la dialectique du signe et de la matière. Avec chaque position, cette dialectique est reformulée par les modèles théoriques de chaque discours. Ainsi, l'introduction de la sémiologie a déplacé l'ambigüité perceptuelle par une ambigüité plus sémantique. Par la suite, les considérations phénoménologiques apportées à la matière ont introduit des notions sensorielles. La nouvelle opposition illustre une apparente incompatibilité de modèles et de sensibilités qui appelle une réconciliation dont 1'horizon n'est pas encore perceptible. Le développement de la dialectique du signe et de la matière à partir des années 1950 est donc l'explication que nous proposons au déplacement conceptuel du débat sur l'apparence et plus particulièrement au niveau de la surface et de l'enveloppe. ______________________________________________________________________________ MOTS-CLÉS DE L’AUTEUR : façade, surface, enveloppe, théorie, conception, dialectique, opposition, matière, signe, construction, représentation, Colin Rowe, Robert Slutzky, Kenneth Frampton, Reyner Banham, Robert Venturi, Denise Scott Brown, David Leatherbarrow, Alejandro Zaera Polo.

I present the results of a comprehensive assessment of companions to 454 solar-type stars within 25 pc. New observational aspects of this work include surveys for (1) very close companions with long-baseline interferometry at the Center for High Angular Resolution Astronomy (CHARA) Array, (2) close companions with speckle interferometry, and (3) wide proper motion companions identified by blinking multi-epoch archival images. I have also obtained and included unpublished results from extensive radial velocity monitoring programs. The many sources utilized enable a thorough evaluation of stellar and brown dwarf companions. The results presented here include eight new companion discoveries, four of which are wide common proper motion pairs discovered by blinking archival images, and four more are from the spectroscopic data. The overall observed fractions of single, double, triple, and higher order systems are 57%±3%, 33%±2%, 8%±1%, and 3%±1%, respectively, counting all stellar and brown dwarf companions. The incompleteness analysis indicates that only a few undiscovered companions remain in this well-studied sample, showing that a majority of the solar-type stars are single. Bluer, more massive stars are more likely to have companions than redder, less massive ones. I confirm earlier expectations that more active stars are more likely to have companions. A preliminary, but important indication is that brown dwarfs, like planets, prefer stars with higher metallicity, tentatively suggesting that brown dwarfs may form like planets when they are companions to stars. The period distribution is unimodal and roughly Gaussian with peak and median values of about 300 years. The period-eccentricity relation shows a roughly flat distribution beyond the circularization limit of about 12 days. The mass-ratio distribution shows a clear discontinuity near a value of one, indicating a preference for twins, which are not confined to short orbital periods, suggesting that stars form by multiple formation mechanisms. The ratio of planet hosts among single, binary, and multiple systems are statistically indistinguishable, suggesting that planets are as likely to form around single stars as they are around components of binary or multiple systems at sufficiently wide separations.

Preservation of large tracts of habitat is often recommended for long-term population viability of area-sensitive species. Large tracts may not always be available. Smaller patches, though not able to contain a viable population individually, may contribute to overall regional population viability if within the small patches pairs could successfully reproduce. By definition, area-sensitive species should have a minimum patch size threshold of habitat below which they will not likely reproduce. Two potential causes for positive relationships between patch size and production are inverse relationships between patch size and brood parasitism and patch size and food availability. My objectives were (1) to determine the minimum patch size thresholds of reproductive success for golden-cheeked warblers (Dendroica chrysoparia), black-and-white warblers (Mniotilta varia), and white-eyed vireos (Vireo griseus); (2) to determine whether thresholds for occupancy, territory establishment by males, or pairing success were indicative of thresholds of reproduction; (3) to determine whether the proportion of pairs fledging brown-headed cowbird (Molothrus ater) young was related to patch size, and (4) to determine the affects of patch size on food availability (i.e., arthropod abundance). The Vickery index of reproductive activity was used to determine reproductive activity of each male or pair and to quantify parasitism occurrences. I collected arthropods using branch clipping to assess the relationship between patch size and arthropod abundance. I found minimum patch size thresholds of reproductive success for golden-cheeked and black-and-white warblers, but not for white-eyed vireos. Minimum patch size of reproductive success was between 15 and 20.1 ha. Minimum patch size thresholds for occupancy, territory establishment by males, and pair formation were not consistent with thresholds for reproductive success. I found no relationships between patch size and cowbird parasitism or patch size and arthropod biomass. Conservation practices for target species based on thresholds of occupancy, territory establishment, or pair formation may not address issues of reproduction. The ability to identify thresholds of reproductive success for target species could be useful in conservation and management in multiple ways including setting goals for retention and restoration of a target species’ habitat patch size.

Thesis (Ed.D.)--University of West Florida, 2007. / Title from title page of source document. Document formatted into pages; contains 194 pages. Includes bibliographical references.

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