Protocolos de sucesso para a maturação in vitro de oócitos de peixe são importantes, uma vez que é necessário para garantir uma fertilização bem sucedida, formação do zigoto, crescimento do embrião e seu completo desenvolvimento. Em algumas espécies, a eficiência deste processo ainda é muito baixa ou restrita a poucas substâncias que podem ser utilizadas. Assim, pesquisou-se a utilização de hormônios alternativos ao protocolo já existente para maturação in vitro de ovócitos de zebrafish. O objetivo foi avaliar a eficiência do extrato de hipófise de carpa (EHC), dos hormônios folículo estimulante (FSH) e luteinizante (LH) para fazer a maturação dos ovócitos estádio III de zebrafish. Os oócitos estádio III foram colocados em meio de cultivo Leibovitz modificado, suplementado com soro fetal bovino e adicionado o hormônio correspondente a seu tratamento (T1-controle; T2-16 μg/ml de EHC; T3- 32 μg/ml de EHC; T4- 48 μg/ml de EHC; T5- 64 μg/ml de EHC; T6- 80 μg/ml de EHC; T7- 0,5 μg/ml de FSH; T8- 0,5 μg/ml de LH e T9- 0,5 μg/ml de FSH e 0,5 μg/ml de LH). A taxa de maturação foi avaliada através da visualização da quebra da vesícula germinal (GVBD). Em todos os tratamentos houve maturação, embora o EHC tenha demonstrado taxas de maturação muito baixas (T2= 12,8%; T3=24,8%; T4=27%; T5=22,7%; T6=9,7%) e inferiores em relação a maior eficiência dos hormônios gonadotrópicos (T7=16%; T8=35%; T9=50%). Além disso foi possível verificar a viabilidade dos oócito através da fertilização in vitro do melhor tratamento (T9) com uma taxa de eclosão e desenvolvimento em larva de 60%. Os resultados da maturação in vitro utilizando estes indutores hormonais em oócitos estádio III de zebrafish mostraram-se promissores, e reforçam as perspectivas para o aprimoramento e uso desta técnica para produção in vitro de embriões viáveis. / Successful protocols for maturation of oocytes are important, as it is necessary for ensuring successful fertilization, zygote formation, embryo growth and full development. In some species the efficiency of in vitro maturation is still very low or is still restricted to a little amount of substances which can be used for the matter. Thus, we studied the use of alternative hormones to the existing protocol for in vitro maturation of zebrafish oocytes. The aim of this study was to evaluate the efficiency of the use of carp pituitary extract (CPE), the follicle stimulating hormone (FSH) and luteinizing hormone (LH) to oocyte maturation stage III of zebrafish. Oocytes stage III were placed in modified Leibovitz culture medium, suplemented with fetal bovine serum and added to the correnponding hormone treatment (T1-control; T2-16 g / ml of CHE; T3 32 g / ml of CHE, T4 - 48 g / ml of CHE; T5- 64 g / ml of CHE; T6- 80 g / ml of CHE; T7- 0.5 g / ml of FSH, T8 0.5 mg / ml of LH and T9- 0.5 g / ml of FSH and 0.5 mg / ml LH). The maturation rate was assessed by the germinal vesicle break down (GVBD). In all cases there was maturation, though the EHC has demonstrated fairly low maturation rate (T2= 12,8%; T3=24,8%; T4=27%; T5=22,7%; T6=9,7%) and lower in relation of the high efficiency presented by the gonadotropic hormones (T7=16%; T8=35%; T9=50%). In addition it was possible to verify the viability of the oocyte through IVF of the best treatment (T9) with a result of 60% of hatching and larvae development rate. The results of maturation in turn using this hormones in stage III oocytes of zebrafish proved promising, and enhance the prospects for improvement and use of this technique for in vitro production of viable embryos.

Asian carp have rapidly expanded their range through much of the Mississippi River Drainage over the past 10 to 15 years. Silver and bighead carp are now the dominant fish species present along several reaches of the Illinois River. The upper Illinois River and shipping canals entering Lake Michigan are of great concern as pathways for Asian carp to enter the Great Lakes. Knowledge of reproductive habitats and dispersal pathways for these species may be valuable for ongoing and future efforts to control these exotic invasives. Previous studies have successfully identified spawning areas of native riverine species via otolith microchemistry, but this technique has not yet been applied to Asian carps. Both stable isotope and trace element ratios have been found to differ significantly among the large rivers of the Mississippi River drainage, enabling identification of natal environment for individual fish. The primary objective of this study was to identify differences in natal river origin and floodplain habitat use through the incorporation of trace elements (Sr:Ca) and stable isotopes (δ18O and δ13C). Silver and bighead carp were collected via electrofishing and trammel netting along four reaches of the Illinois River from the Mississippi-Illinois River confluence at Grafton, IL to the upper segment of the Illinois River upstream of Starved Rock State Park. Sagittal otoliths were removed from both silver and bighead carp collected from each of the four reaches of the Illinois River for analysis of stable isotope ratios and trace element concentrations. Water samples were collected seasonally from the four reaches of the Illinois River and several of its associated floodplain lakes in addition to the Missouri, Upper Mississippi and Middle Mississippi Rivers to validate water signatures of the various river reaches. Results indicated the majority of adult Asian carp caught in the Illinois River originated from the Illinois. However, there was strong evidence indicating roughly twenty percent of captured adults were in fact immigrants from other sources; primarily the Middle Mississippi river and, to a lesser extent, the Missouri River. Stable isotope results indicated that Asian carps primarily used river channel rather than floodplain lake habitats during early life. The findings of this study suggest current Asian carp removal efforts should continue to be primarily directed within the Illinois River, however, the evidence of immigrant silver carp indicate expanding the control efforts into other rivers (Middle Mississippi River and Missouri River) will further support the control of Asian carp within the Illinois River.

The aim of this study was based on laboratory analysis of each parameter, an assessment of livestock interventions, current methods and techniques used in fishing enterprises. Experiments carried out on the market carp in ponds in the system Naděj and the hatcheries under the pond Svět where it was used mechanically and cooked cereals. After processing the fish fillets was done by measuring muscle textural profile. The highest average value of the hardness of the fish ponds was measured in control samples (25,008 ? 3,507 kg). Followed by samples of fish that were unprepared food whatsoever triticale (19,530 ? 2,842 kg). Fish feeding whole triticale heat-treated at 100 ° C (9,990 ? 1,506 kg) and heat-treated triticale mashed at 100 ° C (10,031 ? 1,696 kg) showed similar values of stiffness. The highest average value of the hardness of fish hatcheries was measured in samples of fish that were food whatsoever (20,420 ? 1,456 kg). In addition, followed in descending order of fish samples, which posed a triticale cooked at 100 ° C (11,198 ? 3,334 kg) of wheat at 100 ° C (10,286 ? 1,234 kg), barley at 100 ° C (8,564 ? 1,532 kg), and rye at 100 ° C (7,519 ? 1,993 kg).

The aim of my thesis was to verify the technology of rearing common carp (Cyprinius carpio) with increased levels of omega 3 fatty acids. The technology was tested in 2010 and 2011. A mixture of KP Len and cereal was used for the experiment in 2010. In 2011, the fish were divided into three groups The first group was fed a mixture of KP Len, the second a type of cereal, and the third was kept on natural feed. The technology was carried out under the conditions of the Czech fishery industry. During the entire growing period the course of growth was monitored as part of the framework of regular checks and the parameters of the quality of water was measured. The main descriptive characteristics was the fat content and composition of fatty acids. The combination of KP Len achieved a significantly higher content of PUFA than the group of fish receiving cereals in 2010 and in 2011. When comparing groups of fish fed a mixture of KP Len and those fed natural feed in 2011, no statistically significant differences in PUFA were detected. In 2010 the fish fed a mixture of KP Len reached the highest content of EPA and DHA. On the contrary, in 2011 the significantly highest content was detected in the muscle of fish kept on natural feed.

This thesis was focused on invasive fish species, especially prussian carp (Carassius auratus gibelio) and topmouth gudeon (Pseudorasbora parva) in the Sokolov area and their potential impact on native fish species population in the post-mining water reservoirs. The aim was to compare reservoirs with and without specific maintenance.

No description available.

The aim of this study was to test the influence of bleeding on the flesh quality of common carp. For the assessment of the influence of bleeding on the colour, spectrophotometrical measuring was used. The sensory quality was evaluated to an ISO 8529 standard by a trained staff member of the Institute of Aquaculture and Protection of Water. Both raw and cooked samples were evaluated. Microbiological analysis was done according to ISO 4833 standard and lipid oxidation measured by TBARS. The influence of the bleeding was observed in all colour parameters (L*, a* and b*). The average of L* values was 43.7 +- 1; 42.7 +- 1, a 43 +- 0.9 in bled group and 37.51 +- 0.54; 37.98 +- 0.79; 37.42 +- 0.6 in unbled. The average of a* values was -1.4 +- 0.2; -1.6 +- 0.2; -0.9 +- 0.4 in the bled group and 4.5 +- 2.3; 3.6 +- 1.6; 7.4 +- 1 in the unbled group. A significant difference (p < 0.05) between groups was observed in all days of study in the L* and a* parameter. In the b* parameter there was a difference observed just in the first nine days. A significant difference (p < 0.05) between the bled and the unbled groups was observed in all criteria on the raw sample on the 1st and 3rd days. Cooked samples were significantly different (p < 0.05) just on the 6th day of study. Microbiological results came up to 4 +- 0.2 log CFU.g-1 in the bled group and 6.3 +- 0.1 log CFU.g-1 in the unbled group. The influence of bleeding was shown in lipid oxidation too. At the end of the study TBARS values were 17.8 +- 8.8 ug.kg-1 of the sample in the bled group and 39.9 +- 6.4 ug.kg-1 of the sample in the unbled group. In both groups, exponential growth was shown. The influence of bleeding was observed in all parameters. It can be stated that the bleeding has a positive influence on the flesh quality of common carp. In order to convert these results into practice, further studies to optimize the bleeding process are needed.

Cryopreservation and transplantation of germ cells in fish provides a suitable tool for preserving genetic information. By method of surrogate reproduction, the offspring with characters of the chosen donor can be obtained. In this case of our commercially important species common carp. However, for the successful cryopreservation of the germ cells, a suitable protocol for each species must be established. Several cryoprotectants were tested. The best of them, Me2SO, regarding the viability of spermatogonia, was tested for its different concentrations depending also on the rate of freezing. Further testing, related to the effect of tissue size, incubation time and added sugar, was performed. The result of the assay identified best cryomedium composed of 2.5M dimethylsulfoxide, added sugar of 0.3M glucose, 1.5% BSA and 25nM Hepes dissolved in PBS. The most suitable size of tissue was 100 mg, incubation time was 30 min and coolig rate was -1 ° C/min. This protocol ensures the highest viability rate of cryopreserved spermatogonia of common carp. The second part of the work was to verify the success of the transplantation of cryopreserved and fresh spermatogonia into a suitably chosen recipient, the goldfish, which shares similar reproductive characteristics with carp, but also offers reduction of space requirements or resistance to koi-herpes virus. The transplanted germ cells colonized the germ line and started gametogenesis in 42.5% (cryopreserved spermatogonia) and 52.5% (fresh spermatogonia) goldfish recipients, which demonstrated that the transplantation of cryopreserved spermatogonia of common carp can be successfully achieved.

No description available.

Submitted by Deyse Queiroz (deysequeirozz@hotmail.com) on 2018-05-23T14:25:29Z No. of bitstreams: 1 LEONARDO DA SILVA ARAÚJO - DISSERTAÇÃO PPGSA PROFISSIONAL 2015..pdf: 591080 bytes, checksum: dde6ef5013fd4cf765a80f6b6ba9c760 (MD5) / Made available in DSpace on 2018-05-23T14:25:29Z (GMT). No. of bitstreams: 1 LEONARDO DA SILVA ARAÚJO - DISSERTAÇÃO PPGSA PROFISSIONAL 2015..pdf: 591080 bytes, checksum: dde6ef5013fd4cf765a80f6b6ba9c760 (MD5) Previous issue date: 2015 / A piscicultura é uma atividade desenvolvida no mundo há bastante tempo, na América do Sul o primeiro país a introduzir a piscicultura foi à Argentina, importando, em 1870, os primeiros reprodutores de Carpa comum (Cyprinus carpio) e Carpa Espelho (Cyprinus Carpic). Mais tarde foi introduzida, na região de Bariloche, a Truta arco-íris (Salmo irideus). Objetivou-se com este trabalho fazer um estudo sobre a sustentabilidade da piscicultura no município de Coremas PB. A metodologia utilizada foi através da aplicação de questionários semi estruturados aplicados diretamente com os piscicultores, foram aplicados 30 questionários. Para calcular a sustentabilidade da atividade foram calculados os índices Social Econômico, Tecnológico e Ambiental que foram compor o índice de Sustentabilidade da atividade. O índice social foi de 0,6387, o índice econômico foi de 0,7480, o índice tecnológico de 0,5623, o índice ambiental foi de 0,4733 e por fim o índice de sustentabilidade foi de 0,6053. O índice que mais contribuiu para compor a sustentabilidade foi o econômico, porem, todos os índices se encontram dentro do nível médio de sustentabilidade. Isso significa que a atividade piscicola desenvolvida no município de Coremas PB, necessita de mudanças no sentido de melhorar todos os índices apresentados na pesquisa a fim de alcançar a sustentabilidade tão almejada pelos que praticam essa atividade. / - Fish farming is an activity developed in the world for a long time in South America, the first country to introduce fish farming was Argentina, importing in 1870, the first common carp breeding (Cyprinus carpio) and Mirror Carp (Cyprinus Carpic). It was later introduced in the region of Bariloche, the rainbow trout (Salmo irideus). The objective of this work was to study the sustainability of fish farming in the municipality of Coremas PB. The methodology used was by applying semi-structured questionnaires directly with fish farmers, were applied 30 questionnaires. To calculate the sustainability of the Economic Social activity indices were calculated. Technological and Environmental Sustainability that were composing the index of activity. The social rate was 0.6387, the economic rate was 0.7480, the technological index of 0.5623, the environmental index was 0.4733 and finally the sustainability index was 0.6053. The index that most contributed to compose sustainability was economic; however, all indices are within the average level of sustainability. This means that the piscicola activity developed in the city of Coremas PB, needs changes to improve all indices presented in the survey in order to achieve sustainability as desired by practicing this activity.