Made available in DSpace on 2016-03-07T19:21:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-11. Added 1 bitstream(s) on 2016-03-07T19:25:30Z : No. of bitstreams: 1 000858937.pdf: 237984 bytes, checksum: 61c941e361051e9de4cce1254082d23f (MD5) / O presente estudo avaliou se a concentração de P4 durante o desenvolvimento do folículo ovulatório em protocolo a base de P4/BE e com uma aplicação de GnRH em seu início interfere na prenhez à IATF em vacas Holandesas em lactação. A hipótese é que vacas com maior P4 no dia da aplicação da 1ª dose de PGF2α terão maior fertilidade. Para alterar a P4 os animais (n=594) foram distribuídos aleatoriamente em dois grupos para receberem um ou dois dispositivos intravaginais de P4 (CIDR®, Zoetis, SP, Brasil). O protocolo utilizado foi: no d-11 inserção de 1 CIDR (novo ou previamente usado por 9 dias) + aplicação de 2mg i.m. de BE (Gonadiol®, Zoetis, SP, Brasil) + 100mcg i.m. de GnRH (Cystorelin®, Merial, SP, Brasil); d-4, aplicação de 25mg i.m. de Dinoprost (Lutalyse®, Zoetis, SP, Brasil); d-2, aplicação de 25mg i.m. de Dinoprost + 1mg i.m. de ECP (ECP®, Zoetis, SP, Brasil) + retirada do dispositivo de P4; d0, IATF. Os animais do grupo 2CIDR receberam um CIDR adicional no d-11, o qual foi retirado no d-4. Nos d-11 (n= 117), d-4 (n= 351), d0 (n= 214) e d10 (n= 72) foram colhidas amostras de sangue para determinação da concentração de P4. A P/IA foi determinada através de US nos d32 (DG1) e d60 (DG2). Dados binários foram analisados pelo PROC GLIMMIX, e os dados contínuos pelo PROC MIXED do SAS (foi considerada diferença significativa se P<0,05 e tendência se P<0,1). A P4 não diferiu entre os tratamentos no d-11 (1CIDR= 4,2±0,4 ng/ml; 2CIDR= 4,5± 0,4ng/ml; P>0,1), e no d-4 (1CIDR= 3,5±0,2ng/ml; 2CIDR= 3,8±0,2 ng/ml; P>0,1), porém foi detectada interação entre tratamento e presença de CL no início do protocolo na P4 no d-4 (sem presença de CL e 1CIDR= 2,7±0,3ng/ml; 2CIDR= 3,6±0,3ng/ml, com presença de CL e 1CIDR= 4,3±0,3ng/ml; 2CIDR= 4,0±0,3ng/ml; P<0,05). Não houve diferença na prenhez a IATF e nas perdas gestacionais entre os tratamentos [DG1: 1CIDR= 20,0 ... / The present study evaluated if P4 concentration during ovulatory follicle development in a timed artificial insemination (TAI) protocol based on P4/E2 affects pregnancy per AI in lactating Holstein cows. Our hypothesis is that cows that present higher P4 concentration at PGF2α administration have also higher fertility. To alter P4, animals (n=594) were randomly assigned to receive one or two intravaginals devices of P4 (CIDR®, Zoetis, SP, Brazil). TAI protocol utilized was: d-11 intravaginal device of P4 (new or previously used by 9 days) + 2mg im EB (Gonadiol®, Zoetis, SP, Brazil) + 100mcg im GnRH (Cystorelin®, Merial, SP, Brazil); d-4, 25mg im Dinoprost (Lutalyse®, Zoetis, SP, Brazil); d-2, 25mg im Dinoprost + 1mg im ECP (ECP®, Zoetis, SP, Brazil) + CIDR removal; d0, TAI. Animals in the group 2CIDR received an adicional CIDR at d-11, which was removed at d-4. At d-11 (n=117), d-4 (n=351), d0 (n=214) and d10 (n=72), blood samples were taken from cows for P4 concentration measurements. Pregnancy per AI was determined by ultrasound at d32 (DG1) and d60 (DG2). The binomial data were analyzed using PROC GLIMMIX and continuous data using PROC MIXED of SAS. An effect was considered significant when P<0.05 and tendency when P<0.1. P4 did not differ among treatments at d-11 (1CIDR=4.2±0.4ng/ml; 2CIDR=4.5±0.4ng/ml; P>0.1), and at d-4 (1CIDR=3.5±0.2ng/ml; 2CIDR=3.8±0.2 ng/ml; P>0.1). An interaction was detected between treatment and CL presence at the beginning of TAI protocol in P4 at d-4 (without CL and 1CIDR=2.7±0.3ng/ml; 2CIDR= 3.6±0.3ng/ml, with CL and 1CIDR=4.3±0.3ng/ml; 2CIDR= 4.0±0.3ng/ml; P<0.05). There was no difference among treatments in pregnancy per AI and pregnancy loss between DG1 and DG2 [DG1: 1CIDR= 20,0% (81/310) vs. 2CIDR= 15,7% (65/284); DG2: 1CIDR= 18,2% (74/310) vs. 2CIDR= 13,8% (57/283); Pregnancy loss: 1CIDR= 14,1% (7/81) vs. 2CIDR= 11,5% (7/64); P>0,1]. 37,0% (28/63)...

Orientador: Flavia Thomaz Verechia Pereira / Banca: Cristiana Andrighetto / Banca: Vanessa Gomes Ueno / Resumo: A utilização da transgenia com a proteína fluorescente verde (GFP) como marcador de células de origem fetal nas placentas de clones bovinos servirá de modelo inédito para estudo morfofisiológico e imunológico da interação materno-fetal, visto que possibilitará o seu mapeamento, diferenciando as células fetais das maternas. Tal modelo terá aplicação direta, principalmente porque estes são animais que apresentam problemas em relação ao seu desenvolvimento. Com o auxílio deste modelo, pretende- se verificar o transporte de substâncias entre a mãe e o feto via endocitose, pela imunolocalização das proteínas chamadas de caveolinas. Para tanto foram utilizados 06 bovinos clonados e 30 bovinos de inseminação artificial (IA) com idade até 90 dias de gestação, os quais tiveram seu desenvolvimento interrompido mediante abate humanitário das receptoras e ovariosalpingohisterectomia, com posterior recuperação do útero gestante. Foram coletados os placentônios e o cório. Uma parte das amostras foi recortada e fixada, por imersão, em solução de parafolmaldeído a 4% ou formoldeído a 10% em tampão fosfato de sódio (PBS) a 0,1M pH 7.4, solução de Zamboni (4% de paraformoldeído, 15% de ácido pícrico, em tampão fosfato de sódio a 0,1M pH 7.4), metacarn (60% de metanol, 30% de clorofórmio, e 10% de ácido acético glacial), para verificação da morfologia e realização de imuno-histoquímica para as proteínas caveolinas -1 e -2 (CAV -1 e CAV-2). As caveolinas -1 foram localizadas nos vilos fetais e maternos, mas sua marcação mais forte foi observada no estroma endometrial. As caveolinas -2 tiveram marcação positiva no trofoblasto e membrana corioalantoide, e, especificamente em célula trofoblástica gigante binucleada. Sendo assim, os resultados mostram que a proteína CAV-1 teve uma maior expressão em relação a proteína CAV-2 e que as proteínas CAV-1 e -2 são parte da composição das cavéolas, sendo . / Abstract: The transgenic application of green fluorecent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these are animals that present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytocis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples will be cutted and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS) at 0,1M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 ( -1 CAV and CAV- 2). The caveolinas -1 were found in fetal and maternal villi , but its strongest staining was observed in the endometrial stroma . The caveolinas -2 had positive staining in trophoblast and chorioallantoic membrane , and specifically in giant trophoblastic binucleated cell . therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and ... / Mestre

Orientador: José Luiz Moraes Vasconcelos / Banca: João Carlos Pinheiro Ferreira / Banca: Mario Binelli / Resumo: O presente estudo avaliou se a concentração de P4 durante o desenvolvimento do folículo ovulatório em protocolo a base de P4/BE e com uma aplicação de GnRH em seu início interfere na prenhez à IATF em vacas Holandesas em lactação. A hipótese é que vacas com maior P4 no dia da aplicação da 1ª dose de PGF2α terão maior fertilidade. Para alterar a P4 os animais (n=594) foram distribuídos aleatoriamente em dois grupos para receberem um ou dois dispositivos intravaginais de P4 (CIDR®, Zoetis, SP, Brasil). O protocolo utilizado foi: no d-11 inserção de 1 CIDR (novo ou previamente usado por 9 dias) + aplicação de 2mg i.m. de BE (Gonadiol®, Zoetis, SP, Brasil) + 100mcg i.m. de GnRH (Cystorelin®, Merial, SP, Brasil); d-4, aplicação de 25mg i.m. de Dinoprost (Lutalyse®, Zoetis, SP, Brasil); d-2, aplicação de 25mg i.m. de Dinoprost + 1mg i.m. de ECP (ECP®, Zoetis, SP, Brasil) + retirada do dispositivo de P4; d0, IATF. Os animais do grupo 2CIDR receberam um CIDR adicional no d-11, o qual foi retirado no d-4. Nos d-11 (n= 117), d-4 (n= 351), d0 (n= 214) e d10 (n= 72) foram colhidas amostras de sangue para determinação da concentração de P4. A P/IA foi determinada através de US nos d32 (DG1) e d60 (DG2). Dados binários foram analisados pelo PROC GLIMMIX, e os dados contínuos pelo PROC MIXED do SAS (foi considerada diferença significativa se P<0,05 e tendência se P<0,1). A P4 não diferiu entre os tratamentos no d-11 (1CIDR= 4,2±0,4 ng/ml; 2CIDR= 4,5± 0,4ng/ml; P>0,1), e no d-4 (1CIDR= 3,5±0,2ng/ml; 2CIDR= 3,8±0,2 ng/ml; P>0,1), porém foi detectada interação entre tratamento e presença de CL no início do protocolo na P4 no d-4 (sem presença de CL e 1CIDR= 2,7±0,3ng/ml; 2CIDR= 3,6±0,3ng/ml, com presença de CL e 1CIDR= 4,3±0,3ng/ml; 2CIDR= 4,0±0,3ng/ml; P<0,05). Não houve diferença na prenhez a IATF e nas perdas gestacionais entre os tratamentos [DG1: 1CIDR= 20,0 ... / Abstract: The present study evaluated if P4 concentration during ovulatory follicle development in a timed artificial insemination (TAI) protocol based on P4/E2 affects pregnancy per AI in lactating Holstein cows. Our hypothesis is that cows that present higher P4 concentration at PGF2α administration have also higher fertility. To alter P4, animals (n=594) were randomly assigned to receive one or two intravaginals devices of P4 (CIDR®, Zoetis, SP, Brazil). TAI protocol utilized was: d-11 intravaginal device of P4 (new or previously used by 9 days) + 2mg im EB (Gonadiol®, Zoetis, SP, Brazil) + 100mcg im GnRH (Cystorelin®, Merial, SP, Brazil); d-4, 25mg im Dinoprost (Lutalyse®, Zoetis, SP, Brazil); d-2, 25mg im Dinoprost + 1mg im ECP (ECP®, Zoetis, SP, Brazil) + CIDR removal; d0, TAI. Animals in the group 2CIDR received an adicional CIDR at d-11, which was removed at d-4. At d-11 (n=117), d-4 (n=351), d0 (n=214) and d10 (n=72), blood samples were taken from cows for P4 concentration measurements. Pregnancy per AI was determined by ultrasound at d32 (DG1) and d60 (DG2). The binomial data were analyzed using PROC GLIMMIX and continuous data using PROC MIXED of SAS. An effect was considered significant when P<0.05 and tendency when P<0.1. P4 did not differ among treatments at d-11 (1CIDR=4.2±0.4ng/ml; 2CIDR=4.5±0.4ng/ml; P>0.1), and at d-4 (1CIDR=3.5±0.2ng/ml; 2CIDR=3.8±0.2 ng/ml; P>0.1). An interaction was detected between treatment and CL presence at the beginning of TAI protocol in P4 at d-4 (without CL and 1CIDR=2.7±0.3ng/ml; 2CIDR= 3.6±0.3ng/ml, with CL and 1CIDR=4.3±0.3ng/ml; 2CIDR= 4.0±0.3ng/ml; P<0.05). There was no difference among treatments in pregnancy per AI and pregnancy loss between DG1 and DG2 [DG1: 1CIDR= 20,0% (81/310) vs. 2CIDR= 15,7% (65/284); DG2: 1CIDR= 18,2% (74/310) vs. 2CIDR= 13,8% (57/283); Pregnancy loss: 1CIDR= 14,1% (7/81) vs. 2CIDR= 11,5% (7/64); P>0,1]. 37,0% (28/63)... / Mestre

This study was designed to: 1. Determine the effects of sperm dose and retrograde sperm blockage on mean number accessory sperm/ova. 2. Evaluate the relationship between mean number accessory sperm/ova and fertilization status/embryo quality. 3. Determine if mean number accessory sperm/ova or embryo quality are affected by semen quality. 4. Compare the percentage of morphologically normal accessory sperm with the percentage of normal cells in the inseminate. Using excised reproductive tracts, a French insemination rod housed in a 24-gauge Foley catheter was determined to be effective in blocking retrograde flow of semen following insemination. In a preliminary study, blocked vs conventional inseminations (control) were made using average quality frozen semen at 20 x 10⁶ sperm/dose. Although not different (P > .1), the mean number accessory sperm/ovum was 20 ± 40 (n= 24) and 13 ± 28 (n= 26) for the blocked and control methods, respectively. In Expt. 1, the conventional (control) and blocked system were again compared in a 2x2 factorial using low quality semen at 20 and 40 x 10⁶ sperm/dose. Mean number accessory sperm/ova was not affected by dose, blocking, nor the interaction. Embryo quality was negatively affected by blocking (P < .1), and unaffected by sperm dose. In Expt. 2, embryo quality and accessory sperm numbers were unaffected by a 40 x 10⁶ sperm dose of either average or below average quality semen. However, embryo quality tended to be improved by the average quality semen. Accessory sperm were significantly enriched with morphologically normal cells when compared with those in the inseminate (P < .01). Viable quality embryos (poor thru excellent) had the highest mean number accessory sperm/ovum (16.2 ± 28.9), most unfertilized ova (UFO) contained zero accessory sperm (.27 ± .83) and degenerates embryos were intermediate in number (5.4 ± 8.7). The relationship between embryo quality and accessory sperm number appears to vary in response to semen quality. / Master of Science

Two studies were conducted to investigate the role of the bovine cervix in filtering abnormal sperm. ln Study 1, semen containing high levels of abnormal sperm was vaginally deposited in 12 cows 80 hours after prostaglandin F₂α treatment. At slaughter, 4-, 8- or 12 hours post-insemination, sperm were flushed from the excised uteri with fixative. Pooled across times post-insemination, viability was greater for uterine vs inseminate sperm, based upon vital smears prepared from inseminate and uterine flush samples. Uterine levels of normal sperm, determined by differential interference contrast (DIC) microscopy, were greater than were inseminated. In Study 2, heifers were estrus synchronized in pairs using prostaglandin F₂α, then inseminated with semen containing high levels of abnormal sperm. In each pair, semen of high viability (Experiment 1, n=10) or low viability (Experiment 2, n=6) was deposited vaginally in one heifer and within the uterine corpus of the other. Using DIC microscopy, viability and morphology were coincidentally determined for sperm in samples from the fixed inseminate and the retrograde mucus, vaginal mucus, cervix, uterus and in vitro incubation of inseminate recovered and fixed 12 hours post-insemination. Uterine sperm quality did not differ between insemination sites, except lower uterine levels of live abnormal sperm alter intrauterine vs vaginal insemination of low viability semen probably due to disproportionately low viability of one abnormality. Sperm viability was enhanced and morphology unchanged in the uterus vs low viability inseminate, while sperm viability was unchanged and abnormal sperm subtly reduced in uterus vs high viability inseminate. Greater levels of live and live normal sperm were found deeper between cervical folds than at apical aspects of folds. Vaginal mucus sperm viability was lower compared to other tract locations and inseminate, especially after high viability insemination. Compared to inseminate viability, retrograde mucus sperm viability was high after vaginal insemination and low alter intrauterine insemination. Differential death of abnormal vs normal sperm neither with incubation of insemination in vitro nor, presumably, in vivo. Results show little evidence of cervical filtration based upon sperm morphology. Sperm retention in the female tract was predominantly related to sperm viability with only very subtle morphology effects. / Ph. D.

Call number: LD2668 .T4 1985 M52 / Master of Science

Made available in DSpace on 2014-06-11T19:35:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-19Bitstream added on 2014-06-13T20:46:10Z : No. of bitstreams: 1 oliveira_lz_dr_jabo.pdf: 1430091 bytes, checksum: 95930fc0477f477381f0a00200fbbae4 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Tem sido frequentemente demonstrado que variadas taxas de concepção podem ser obtidas de acordo com o sêmen utilizado, evidenciando uma importante variabilidade individual do reprodutor com relação à fertilidade. Além disso, as grandes dimensões dos rebanhos, associado ao intenso e crescente uso dos protocolos de IATF no Brasil, tem estimulado, cada vez mais, a prática do descongelamento simultâneo de diversas palhetas de sêmen em um mesmo descongelador. Entretanto, sob essas condições, o banho-maria do descongelador poderia atuar como ambiente de incubação para o sêmen, o que poderia influenciar a viabilidade do espermatozoide. Assim, o presente estudo teve por objetivos: 1) determinar o efeito da sequência de inseminação, após descongelamento simultâneo de diversas palhetas de sêmen, na taxa de concepção e na qualidade seminal; 2) investigar a fertilidade in vivo, bem como diversas características espermáticas in vitro, do sêmen de diferentes reprodutores utilizados no mesmo programa de IATF; 3) identificar, dentre as diversas análises laboratoriais realizadas, o grupo de características espermáticas consideradas importantes na predição da taxa de concepção. Para tal, o presente experimento foi realizado em duas etapas. A primeira etapa consistiu em um estudo a campo delineado para determinar a taxa de concepção após IATF de fêmeas pós-parto da raça Nelore (n=947), utilizando sêmen de três partidas de cada um dos três touros da raça Angus (n=9). A segunda etapa consistiu em um estudo laboratorial delineado para investigar diversas características espermáticas das doses de sêmen utilizadas no respectivo programa de IATF, utilizando as mesmas condições de descongelamento do estudo a campo. As seguintes análises laboratoriais foram realizadas: análise computadorizada do movimento... / It has been reported that different conception rates can be obtained according to the semen used, indicating a significant variability regarding sire fertility. Furthermore, the large size of reproductive herds associated with the intense use of Timed-AI protocols in Brazil, has stimulated the routine practice of simultaneous thawing of multiple semen straws in the same thawing-bath. However, under these conditions, the thawing-bath could act as an incubator environment for the semen, which could influence the sperm viability. Thus, this study aimed to: 1) determine the effect of sequence of insemination after simultaneous thawing of multiple semen straws on conception rate and semen quality; 2) investigate the in vivo fertility, as well as several in vitro sperm characteristics, from different sires used in the same Timed-AI program; 3) identify, among the several in vitro sperm variables evaluated in laboratory, the group of sperm characteristics considered important in the prediction of conception rate. Hence, this study was conducted in two experiments. The first experiment was a field study designed to determine conception rate after Timed-AI of suckled multiparous Nelore cows (n = 944) using three semen batches from three Angus bulls (n = 9). The second experiment was a laboratory study designed to investigate several sperm characteristics of semen used in the Timed-AI program, using the same thawing conditions applied in the field experiment. The following laboratory analyses were performed: computer assisted semen analysis, hypo-osmotic swelling test, sperm resistance after 2 hours of thermal incubation, sperm morphology, integrity of plasma and acrosomal membranes, plasma membrane stability, lipid peroxidation, sperm morphology and chromatin condensation. According to the present experiment, it was concluded that... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:28:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-20Bitstream added on 2014-06-13T19:57:45Z : No. of bitstreams: 1 perdigao_a_me_botfmvz.pdf: 431185 bytes, checksum: b1a2ca863017a5e14a6400e6da42dc9f (MD5) / O objetivo foi avaliar os efeitos da duração de protocolos de adaptação a dietas de alto teor de concentrado sobre o comportamento ingestivo, desempenho, características de carcaça e saúde ruminal de bovinos Nelore confinados. Machos não castrados da raça Nelore (n = 120), com 352,03 ± 19,61 kg de peso vivo (PV), foram mantidos por 84 dias em 24 baias. O delineamento experimental foi em blocos casualizado, com arranjo fatorial 2 × 2 (protocolos e durações), constituindo os seguintes tratamentos: adaptação em escada por 6 (E6) ou 9 dias (E9) e protocolo de restrição por 6 (R6) ou 9 dias (R9), sendo 6 baias por tratamento. Houve efeito de protocolo de adaptação no ganho de peso médio e ingestão de massa seca (IMS) em porcentagem do PV, sendo maiores na adaptação em escada no período final. Para incidência de rumenite, teve efeitos de duração e protocolo, sendo o protocolo R9 com maior lesão da parede ruminal. Na avaliação morfológica do epitélio ruminal foi encontrado efeito de duração na fase de adaptação para área média de papilas. Nas variáveis histológicas houve diferença entre as fases (adaptação e terminação) para altura, largura e espessura de queratina das papilas ruminais. No comportamento ingestivo, todos os itens avaliados apresentaram efeitos significativos, destacando-se, a IMS na adaptação e terminação, e variação de consumo na adaptação, em que o protocolo de escada proporcionou maior ingestão e menor variação. Contudo, na adaptação de Bovinos Nelore confinados alimentados com dietas de alto teor concentrado, recomenda-se o protocolo de escada com 9 dias de duração / The objective was to evaluate the effects of duration of protocols to adapt to diets high concentrate diets on feeding behavior, performance, carcass characteristics and rumen health of confined Nellore. Uncastrated male Nelore (n = 120), with 352.03 ± 19.61 kg, were kept for 84 days in 24 bays. The experimental design was randomized blocks, with 2 × 2 factorial arrangement (protocols and durations), constituting the following treatments: adaptation for in steps 6 (E6) or 9 days (E9) and protocol restriction for 6 (R6) or 9 days ( R9), being 6 pen per treatment. There was no effect of protocol adaptation in average weight gain and dry matter intake (DMI) as a percentage of BW, with greater adaptation ladder in the final period. Incidence of ruminites had significant effects and protocol, with the largest lesions R9 protocol with the rumen wall. In the morphology of the ruminal epithelium was not found in the effect of duration adaptation phase to average area of papillae. In histological variables was no difference between the phase (adaptation and finishing) for height, width and thickness of keratin of rumen papillae. In feeding behavior, all items presented significant effects, especially in DMI during adaptation and finishing, and variation in intake in the adaptation, where the step up protocol provided higher intake and lower variation. All in all, in the adaptation for feedlot Nellore cattle fed to high-concentrate diets, indicated the 9 days in steps

Made available in DSpace on 2014-06-11T19:28:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-18Bitstream added on 2014-06-13T18:48:18Z : No. of bitstreams: 1 sanchesjunior_cp_me_botfmvz.pdf: 521404 bytes, checksum: c19df2c844afe1ed0e76070de6937621 (MD5) / Objetivou-se com este estudo avaliar a utilização de dois dispositivos intravaginais de P4 (CIDR®, Zoetis) em vacas Holandesas em lactação sem CL e com P4 ≤1,0 ng/mL no início de um protocolo de sincronização da ovulação à base de E2/P4 para IATF (exp 1) ou TETF (exp 2) no sucesso da prenhez. Estes experimentos foram realizados em 9 fazendas, sendo 4 destinadas a IATF (n = 341) e 5, a TETF (n = 356). O protocolo utilizado nos experimentos foi: d-10 2mg IM BE (2,0mL de Estrogin, Farmavet, SP, Brasil) + CIDR (1,9g de P4, CIDR®, Zoetis, SP, Brasil); d-3 25mg IM Dinoprost Trometamina (5,0mL de Lutalyse®, Zoetis, SP, Brasil); d-2 remoção do CIDR + 1mg IM de Cipionato de Estradiol (0,5mL ECP®, Zoetis, SP, Brasil); d 0 IATF (exp1) ou d 7 TETF (exp 2). No d-10, os animais sem corpo lúteo à ultrassonografia (US) foram distribuídos aleatoriamente para receberem 1 CIDR (1CIDR) ou 2 CIDR (2CIDR). O diâmetro do folículo ovulatório [d0 (exp 1)] e presença de CL [d7 (exp 1 e 2)] foram avaliados por US. Amostras de sangue foram colhidas para dosagem de P4 nos dias -10, -3 e 7. Foram consideradas sincronizadas vacas com CL 7 dias após a IA (d7) ou com CL no momento da TE, consistente com a ovulação do folículo ovulatório; no exp 2, apenas vacas com CL (d7) que receberam embrião foram utilizadas. Diagnósticos de gestação foram realizados por US aos 32 e 60 d. Para avaliar as variáveis binomiais, foi utilizado o PROC GLIMMIX, e as contínuas, o PROC MIXED. Efeitos foram considerados significantes quando P ≤0,05 e tendência quando P >0,05 e P ≤0,10. Não houve efeito do tratamento na taxa de sincronização [1CIDR = 74,1% (329/451) vs. 2CIDR = 71,1% (298/423)]. A concentração de P4 no d-10 não diferiu entre os tratamentos (1CIDR = 0,59 ± 0,12 ng/mL vs. 2CIDR = 0,68 ± 0,12 ng/mL)... / The aim of this study was to evaluate the use of two progesterone (P4) intravaginal devices (CIDR ®, Zoetis) in lactating Holstein cows without CL with P4 ≤ 1.0 ng / mL at the start of a E2/P4 ovulation synchronization protocol for TAI (exp 1) or TET (exp 2) on pregnancy success. These experiments were conduced in 9 farms, 4 destined to TAI (n = 341) and 5 TET (n = 356). The protocol used in the experiments was: d-10 2 mg IM EB (2.0 mL Estrogin, Farmavet, SP, Brazil) + CIDR (1.9 g of P4, CIDR ®, Zoetis, SP, Brazil); d-3 25 mg dinoprost tromethamine IM (5.0 mL Lutalyse ®, Zoetis, SP, Brazil), d-2 CIDR removal + 1 mg estradiol cypionate IM (0.5 mL, ECP ®, Zoetis, SP, Brazil); TAI d0 (exp 1) or TET d7(exp 2). On d-10, animals without a corpus luteum detected by ultrasonography (US) were randomly divided into groups to receive 1 CIDR (1CIDR) or 2 CIDR (2CIDR). The ovulatory follicle diameter [d0 (exp 1)] and presence of CL [d7 (exp 1 and 2)] were evaluated by US. Blood samples were collected for P4 measurement on days -10, -3 and 7. Were considered synchronized cows when they had CL 7 days after AI (d7) or CL at the time of ET, consistent with ovulation of detected ovulatory follicle; In exp 2 only cows CL (d7) receiving embryo were used. Pregnancy diagnosis were performed using US on days 32 and 60. Was used to evaluate binomial variables PROC GLIMMIX and PROC MIXED for continuous. Effects were considered significant when P ≤ 0.05 and tendency when P> 0.05 and P ≤ 0.10. There was no effect of treatment on synchronization rate [1CIDR = 74.1% (329/451) vs. 2CIDR = 71.1% (298/423). P4 concentration on d-10 did not differ between treatments (1CIDR = 0.59 ± 0.12 ng/mL vs. 2CIDR = 0.68 ± 0.12 ng/mL). P4 concentration was higher for 2CIDR treatment (2.14 ± 0.31 ng / mL) compared to 1CIDR (1.71 ± 0.28 ng / mL) at d-3 (PGF moment)... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:29:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-13Bitstream added on 2014-06-13T20:38:41Z : No. of bitstreams: 1 ranieri_al_me_jabo.pdf: 416692 bytes, checksum: 8c5b1d1a825427d9d4b8b9ab52602486 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo deste trabalho foi avaliar o efeito do meloxicam sobre a taxa de concepção inicial (TCi), final (TCf) e perda gestacional (PG) de vacas Holandesas de alta produção submetidas à Transferência de Embrião (TE) Inseminação Artificial (IA) e Inseminação Artificial em Tempo Fixo (IATF). Na TE foram utilizadas, como receptoras de embrião, 492 vacas em lactação repetidoras de serviço. Na IA (n=312) e IATF (n=336) foram incluidas no estudo somente fêmeas recém paridas (com pelo menos 60 dias em lactação). Todas as fêmeas foram avaliadas e alocadas em um de dois grupos experimentais: Grupo Controle (GC) e Grupo Tratado (GT). O tratamento consistiu na administração i.m. de meloxicam injetável 2% (0,5mg/kg de peso) nos dias 7, 8 e 9 após a inovulação das receptoras de embrião, e nos dias 14, 15 e 16 da possível gestação das fêmeas submetidas a IA e IATF. Amostras de sangue foram colhidas nos D14 e D21 para posterior dosagem de progesterona (P4). O diagnóstico de gestação ocorreu aos 25-38 e 39-52 dias. O tratamento com o antiinflamatório não incrementou as TCi, TCf e tampouco diminuiu a PG em nenhuma das três biotecnologias. Contudo, observou-se que receptoras de embrião inovuladas com embriões congelados/descongelados do GT tiveram TCi e TCf menores que as do GC (GT= 25,8% v. GC=37,8%) .Não houve diferença na concentração sérica de progesterona de vacas do GC e do GT no D21. Contudo, houve efeito da estação do ano sobre as concentrações de P4 no D14 e D21 tanto na TE como na IA e IATF. Ainda, vacas gestantes apresentaram maiores concentrações de P4 que vacas não gestantes no D21. Conclui-se que o tratamento com meloxicam não incrementou as TCi, TCf, tampouco reduziu a PG de vacas Holandesas de alta produção / The aim of this study was to evaluate the effect of meloxicam on initial conception rate, final conception rate and pregnancy loss of high-producing Holstein cows submitted to embryo transfer (ET), artificial insemination (AI) and timed artificial insemination (TAI). On ET, 492 repeat breeders in milk were used as recipients. On AI (n=312) and TAI (n=336) only freshly calved cows (with at least 60 days in milk) were included in the study. The animals were evaluated and allocated in one of two experimental groups: Control Group (CG) and Treated Group (TG). The treatment consisted on administration of meloxicam 2% i.m. (0.5mg/kg of weight) on days 7, 8 and 9 after inovulation of embryo recipients, and on days 14, 15 and 16 of the possible pregnancy of females submmited to AI and TAI. Blood samples were collected on D14 and D21 for progesterone assay. The diagnosis of pregnancy was done on days 25-38 and 39-52. The treatment with the antiinflamatory did not increase initial conception rates and final conception rates, nor decreased pregnancy loss of animals in all three biotechnologies. However, recipients of TG inovulated with frozen/thawed embryos had lesser initial and final conception rates than CG (TG= 25.8% v. CG=37.8%) There was no difference on progesterone serum concentration of cows from TG and CG on D21. However, there was a season effect on P4 concentrations on D14 and 21 for ET, AI and TAI. Pregnant cows had higher P4 concentrations than non-pregnant cows. In conclusion, the treatment with meloxicam did not increase initial and final conception rates, neither decreased pregnancy loss of high producing Holstein cows