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Indutores de resistência e seus efeitos sobre Bipolaris sorokiniana e Azospirillum brasilense em trigo: custos adaptativos e ecológicoIurkiv, Luciana 12 September 2016 (has links)
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Previous issue date: 2016-09-12 / The induced resistance efficiency in the control of pathogens is recognized, however there are costs related to its use that are still little researched, as the case of fitness and ecological costs. This research aims to verify the efficiency of use of the acibenzolar-S-methyl (ASM), mannanoligosaccharide (MOS) and B. cereus inductors in the control of spot blotch wheat disease, and its relation with the induction of enzymes related to the plant defense, as well as the interference on gas exchanges, on the non-target microrganisms A. brasilense, and on the crop production. The experiment was conduced in green house growing at the Núcleo de Estações Experimentais of the Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon Campus. The experimental design was made in randomized block design in a factorial schema 4 x 2 x 2, with four replications. The first factor "Resistance Inductors" was constituted by ASM, MOS, B. cereus inductors and water (control). The second factor "Pathogen" was constituted by the presence or absence of B. sorokiniana pathogen. The third factor "PGPR" was made up by the presence or absence of the PGPR A. brasilense. The seeds were inoculated with A. brasilense 24 h before sowing. The treatment with the resistance inductors was done 25 days after the plant emergency and 24 h after it, the inoculation with the pathogen was done. Evaluations for the quantification of peroxidase enzyme, phenylalanine ammonia-lyase and β-1.3-glucanase enzymes activities were done at 0 h, 24 h, 48 h, 72 h and 96 h after treatment. Gas exchanges were evaluated at 0 h, 24 h, 48 h, 72 h, 96 h and 120 h after treatment. The quantification of diazotrophic was made in the implementation of the experiment, at the moment of the use of the resistance inductors and in the flowering. At the flowering, biometric evaluations were performed, and at the end of the cycle, production analysis were held. The use of resistance inductors was efficient in the control of spot blotch wheat disease. The treatment with inductors did not show any interference on the endophytic diazotrophic microrganism. Higher activity of peroxidases for the MOS treatment was observed, that presented high efficiency on
the control of the disease. No relation between the activities of phenylalanine ammonia lyase and β-1.3-glucanase and the control of spot blotch was observed. A higher tax of liquid assimilation of CO2 (A) in the absence of the pathogen and presence of the A. brasilense was observed, however, MOS reduced the “A”. The internal concentration of CO2 and the leaf transpiration showed as superior in the treatment with the absence of the pathogen. The stomatal conduce was affected by MOS treatment. The length of the flag leaf and leaf area were affected by ASM use. But, the total weight of grain did not suffer interference of the treatments, in spite of the weight of 100 grains to have been superior to ASM. / É reconhecida a eficiência da indução de resistência no controle de fitopatógenos, no entanto, existem custos relacionados à sua aplicação ainda pouco investigados, como é o caso dos custos adaptativos e ecológicos. No presente trabalho objetivou-se verificar a eficiência da aplicação dos indutores acibenzolar-S-metil (ASM), mananoligossacarídeo fosforilado (MOS) e Bacillus cereus no controle de mancha marrom em trigo, e sua relação com a indução de enzimas relacionadas a defesa, assim como a interferência sobre as trocas gasosas, sobre o microrganismo não alvo Azospirillum brasilense, e a produção da cultura. O experimento foi conduzido sob cultivo protegido no Núcleo de Estações Experimentais da Universidade Estadual do Oeste do Paraná, Campus de Marechal Cândido Rondon. O delineamento experimental foi em blocos ao acaso em esquema fatorial 4 x 2 x 2, com quatro repetições. O primeiro fator “Indutores de Resistência” foi constituído pelos indutores ASM, MOS, Bacillus cereus e a testemunha Água. O segundo fator “Patógeno” foi constituído pela presença ou ausência do patógeno Bipolaris sorokiniana. O terceiro fator “BPCV” constituiu-se pela presença ou ausência da bactéria promotora de crescimento vegetal A. brasilense. As sementes foram inoculadas com A. brasilense 24 h antes da semeadura. O tratamento com os indutores de resistência foi realizado 25 dias após a emergência e 24 horas após foi realizada a inoculação com o patógeno. Foram realizadas avaliações para quantificação das enzimas peroxidase, fenilalanina amônia-liase e β-1,3-glucanase às 0 h, 24 h, 48 h, 72 h e 96 horas após o tratamento. As trocas gasosas foram avaliadas às 0 h, 24 h, 48 h, 72 h, 96 h e 120 horas após o tratamento. A quantificação de diazotróficos foi realizada na implantação do experimento, no momento da aplicação dos indutores de resistência e no florescimento da cultura. No florescimento foram realizadas análises biométricas e no final do ciclo, análise dos componentes da produção. A aplicação dos indutores de resistência foi eficiente no controle da mancha marrom em trigo. Os tratamentos indutores não apresentaram interferência sobre os microrganismos diazotróficos
endofíticos. Observou-se maior atividade de peroxidases para o tratamento MOS o qual apresentou grande eficiência no controle da doença. Não observou-se relação entre a atividade de fenilalanina amônia-liase e β-1,3-glucanase e o controle de mancha marrom. Observou-se maior taxa de assimilação líquida de CO2 (A) na ausência do patógeno e presença de A. brasilense, porém MOS reduziu a A. A concentração interna de CO2 e a transpiração foliar apresentaram-se superiores nos tratamentos com ausência do patógeno. A condutância estomática foi afetada pelo tratamento MOS. O comprimento da folha bandeira e a área foliar foram afetados negativamente pela aplicação de ASM. Porém, a massa total de grãos não sofreu interferência dos tratamentos, apesar de a massa de 100 grãos ter sido superior para ASM.
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Detecção de Bacillus cereus em leite e avaliação da germinação de seus esporos à temperatura ambiente e sob refrigeração após processo de fervura / Detection of Bacillus cereus in milk and evaluation of the germination of its spores to the ambient and refrigeration temperatures after process of boilMilena Martinelli Watanuki 25 June 2008 (has links)
A análise microbiológica atua como ferramenta fundamental para a obtenção de dados sobre a qualidade, sanidade, higiene e segurança na produção de alimentos; desta forma, tem sido adotada na indústria alimentícia para o controle de qualidade. Por sua composição completa e balanceada, o leite é um substrato ideal para o desenvolvimento de diversos grupos de microrganismos. Com o objetivo de pesquisar bactérias da espécie Bacillus cereus em amostras de leite fluido, bem como a capacidade de germinação de esporos e a multiplicação dessa bactéria após processo de fervura, com manutenção das amostras à temperatura ambiente e à temperatura de refrigeração por períodos de 1, 2, 4, 6, 8, 10 e 12 horas, foram analisadas 75 amostras de leite, conforme as metodologias recomendadas por Silva et al. (2007). Destas, 46 amostras (61,3%) mostraram-se com algum grau de contaminação pela bactéria antes de serem submetidas à fervura. Por sua vez, as amostras mantidas à temperatura ambiente após a fervura, tiveram suas contagens bacterianas, principalmente a partir da 8a hora, superiores à contagem inicial, inclusive atingindo níveis capazes de desencadear uma toxinfecção alimentar, demonstrando a ocorrência da germinação dos esporos e a multiplicação das células vegetativas. Por outro lado, alíquotas dessas mesmas amostras mantidas sob refrigeração (7ºC) não atingiram populações preocupantes, enfatizando, desse modo, a importância da necessidade da refrigeração do leite após a fervura. / The microbiological analysis acts as basic tool for the attainment of data on the quality, health, hygiene and security in the food production, in such a way, she has been adopted in the nourishing industry for the quality control. For its complete and balanced composition, milk is an ideal substratum for the development of diverse groups of microorganisms. With the objective to search cereus bacteria of the Bacillus species in fluid milk samples, as well as the capacity of germination of spores and the multiplication of this bacterium after boil process, with maintenance of the samples to the ambient temperature and the temperature of refrigeration for periods of 1, 2, 4, 6, 8, 10 and 12 hours, 75 milk samples had been analyzed, as the methodologies recommended for Silva et al. (2007). Of these, 46 samples (61.3%) had revealed with some degree of contamination for the bacterium before being submitted to the boil. In turn, the samples kept to the ambient temperature after the boil, had its bacterial countings, mainly from 8a hour, superiors to the initial counting, also reaching levels capable to unchain an alimentary toxinfection, demonstrating to the occurrence of the germination of the spores and the multiplication of the vegetative cells. On the other hand, aliquot of these same samples kept under refrigeration (7ºC) had not reached preoccupying populations, emphasizing, in this manner, the importance of the necessity of the refrigeration of milk after the boil.
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Estudo de biofilmes e células planctônicas de Bacillus cereus frente a um sanificante à base de composto de quaternário de amônio utilizado na indústria de laticínios / Study of Bacillus cereus biofilmes and planktonic cells front to a quaternary ammonium based sanitizer used in the dairy industryAna Cláudia Ribeiro Rossi 11 August 2008 (has links)
O Bacillus cereus é um patógeno amplamente distribuído na natureza, relacionado a intoxicações alimentares e causador da coagulação doce do leite. A intoxicação pode ocorrer após a ingestão de alimentos nos quais a bactéria produziu a toxina. Em laticínios, o B. cereus é problemático por sua habilidade de formar esporos resistentes ao calor e sobreviver aos tratamentos térmicos, como a pasteurização e UHT. As espécies de Bacillus são freqüentemente isoladas de leite pasteurizado e UHT e evidências indicam que os biofilmes formados nas superfícies dos equipamentos de processamento são uma das principais fontes de contaminação. No biofilme, as células ou esporos aderidos, têm aumentada sua resistência e podem resistir ao processo de sanificação, se tornando focos de disseminação de bactérias no processo. A matriz polimérica do biofilme reage e neutraliza os agentes sanificantes, expondo as bactérias em seu interior a doses subletais, podendo levar a aquisição de resistência. Neste estudo, biofilmes de células vegetativas B. cereus foram desenvolvidos na superfície de aço inoxidável, tipo 304, com filme de condicionamento de leite. A adesão média das células foi de 1,4 x 105UFC/cm2. Também foram desenvolvidos biofilmes de esporos, com adesão média em aço inoxidável de 1,4 x 104UFC/cm2. Foi avaliada a resistência das células vegetativas e esporos de B. cereus em biofilmes, ao processo simulado de higienização por clean in place (CIP) realizado em laticínios. Após a limpeza a adesão das células vegetativas em aço inoxidável foi reduzida a aproximadamente 1CFU/cm2. Após a sanificação, os resultados permaneceram inalterados. A contaminação remanescente foi relacionada com a maior resistência das células nos biofilmes formados. Após a limpeza e sanificação a adesão média dos esporos foi de 8,9 x 101 UFC/cm2 e 3,3 x 101 UFC/cm2, respectivamente. Tendo em vista este resultado, foi observado que esporos de B. cereus são mais difíceis de remover de superfícies de aço inoxidável do que células vegetativas, com procedimentos CIP. Finalmente, foi realizada a exposição de células vegetativas de B. cereus a três concentrações subletais (3,9; 1,8 e 0,9µg/mL) de cloreto de alquil amidopropil dimetil benzil amônio para avaliação da adaptação pelo método da concentração inibitória mínima (CIM). A aquisição de resistência foi avaliada pela comparação do valor inicial de CIM (7,4µg/mL), com os valores finais, após 28 dias de exposição. Após o período de adaptação, o valor de CIM encontrado foi o dobro do original (14,9µg/mL) indicando aquisição de resistência ao sanificante pela cepa. Foi constatado ainda que o cultivo da cepa na menor concentração subletal apresentou resultados mais coerentes, sugerindo que exposição a doses subletais mais brandas resulta em uma maior resposta adaptativa. / Bacillus cereus is a pathogen widely distributed in the environment and a serious problem in the dairy industry because of its ability to form heat-resistant spores that can survive pasteurization and UHT treatments. This bacteria is responsible for foodborne diseases in man due to production of toxins that may cause gastroenteritis, diarrhoea and vomiting. In addition, B. cereus is responsible for spoilage of pasteurized milk and cream referred to as \"sweet curdling\". Bacillus strains are frequently isolated from pasteurized and UHT treated milk and evidence indicates that biofilm formed on processing equipment surfaces are major sources of milk contamination. In biofilms, adherent cells and spores acquire increased resistance and persist to the sanitization process, becoming sources of bacteria dissemination in food processing. The extracellular polymeric matrix neutralizes the sanitizing agents and exposing the bacteria in biofilms to sublethal concentrations can lead to an increased resistance. In this study, biofilms of B. cereus vegetative cells were developed in a 304 stainless steel (SS) surface with a milk conditioning film. The mean adhesion of cells on SS surface was 1.4 x 105CFU/cm2. Biofilms of B. cereus spores were also developed, and the mean adhesion found was 1.4 x 104CFU/cm2. The resistance of B. cereus vegetative cells and spores in biofilms to a simulated clean-in-place (CIP) procedure used in dairy industry was evaluated. After cleaning procedure, the population of vegetative cells in the SS surface was reduced to approximated 1 CFU/cm2. After sanitizing procedure, the cell count remained unaltered. The remaining contamination was related with the increased resistance of cells in the developed biofilms. After cleaning and sanitizing procedures, the mean of spore adhesion found was 8.9 x 101 CFU/cm2 and 3.3 x 101 CFU/cm2, respectively. These results show that B. cereus spores are more difficult to remove from SS surfaces than vegetative cells using CIP procedures. Finally, B. cereus vegetative cells were exposed to three different sublethal concentrations (3.9; 1.8 and 0.9µg/mL) of alkyl amidopropyl dimethyl benzyl ammoniun cloride, for evaluation of adaptation, using the Minimum Inhibitory Concentration (MIC) test. The acquired resistance was evaluated by comparing the initial MIC value (7.4µg/mL) with final MICs, after 28 days of exposure. After the adaptation period, with exception of an experiment where the MIC was threefold the original value (29.8µg/mL), in the other experiments the MICs found was double (14.9µg/mL) of the original MIC, indicating acquisition of resistance by the B. cereus strain. It was observed that the growth of cells in the mildest sublethal concentration (0.9µg/mL) resulted in more consistent effects, suggesting that the exposure to mildest sublethal concentrations results in higher adaptive responses.
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Eneterotoxigenic Bacillus cereus and Bacillus thuringiensis Spores in U.S. retail SpicesHariram, Upasana 18 March 2015 (has links)
Bacillus cereus is a ubiquitous organism and a potential foodborne pathogen that can cause two types of gastrointestinal diseases: emesis and diarrhea. The emetic syndrome is caused by a heat and acid stable peptide toxin that is pre-formed in food, while the diarrheal syndrome is associated to two 3-protein, heat labile enterotoxin complexes that are formed in the intestine after ingestion of the organism. There are many reports on the isolation and characterization of Bacillus cereus from various foods, however there are no studies on the levels, toxigenicity and physical characteristics of B. cereus isolated from U.S. retail spices. A huge part of spices sold in the U.S. are imported from developing nations. Developing nations lack hygienic practices during processing and packaging of spices, due to which there is a high chance of imported spices being contaminated with B. cereus. Therefore, the main objective of this thesis work was to characterize B. cereus spores from U.S. retail spices. Levels of aerobic spores and B. cereus spores were determined. B. cereus spores were further analyzed for their enterotoxigenic ability, growth characteristics and physical spore characteristics.
In the 247 spice samples analyzed 77 were found to contain B. cereus, while 11 were positive for B. thuringiensis. Eighty four of the 88 spices tested possessed either one of the enterotoxin genes. None of the isolates tested positive for the emetic toxin (ces) gene. Seventy five of the B. cereus isolates grew at 12 °C, although only two isolates grew well at 9 °C.
Seven selected diarrheal B. cereus spore strains had D95-values ranging from 0.64-3.53 min while the two emetic strains had D95-values of 7.04 min and 6.64 min. B. cereus grew well in pre-cooked rice. After 48 h, counts of 1.26 X 107 and 3.8 X 107 B. cereus/ 10 g were obtained in pre-cooked rice maintained at 17 °C and 20 °C respectively. At 12 °C, counts did not reach 104 CFU/ 10g even after 48 h of incubation. The aerobic mesophilic bacterial population and B. cereus population of 0.1% crushed pepper in pre-cooked rice over a period of 48h at temperature 20 °C and 17 °C were also analyzed. Counts of B. cereus in pepper rice samples reached a maximum of 1600 MPN/ 10 g and 1100 MPN/ 10 g at 20 °C and 17 °C respectively while the aerobic mesophilic counts per 10 g were 2.4 X 108 and 4.4 X 106 at these temperatures. The low B. cereus counts and high aerobic mesophilic population indicates competition of nutrients in cooked rice by background flora other than B. cereus.
The physical spore characteristics of five B. cereus and 3 B. thuringiensis strains were studied using transmission electron microscopy (TEM). Tubular, whip-like appendages were present in four B. cereus and two B. thuringiensis, while all seven isolates possessed exosporia.
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Reducing Sediment and Bacterial Contamination in Water Using Mucilage Extracted from the <em>Opuntia ficus-indica</em> CactusButtice, Audrey Lynn 30 March 2009 (has links)
Throughout the past decade an increased amount of attention has been drawn to the water contamination problems that affect the world. As a result, a variety of purification methods targeted at communities in developing countries have surfaced and, although all have contributed to the effort of improving water quality, few have been accepted and sustained for long term usage. Case studies indicate that the most beneficial methods are those which use indigenous resources, as they are both abundant and readily accepted by the communities. In an attempt to make a contribution to the search for water purification methods that can serve in both developed and developing countries, two fractions of mucilage gum, a Gelling (GE) and a Non-Gelling (NE) Extract, were obtained from the Opuntia ficus-indica cactus and tested as a flocculating agent against sediment and bacteria suspended in surrogate ion-rich waters. Diatonic ions are known to influence both cell binding and mucilage properties, causing CaCl2 to be tested as a flocculating agent alone and in conjunction with mucilage. Column tests were utilized to determine the settling rates of contaminant removal from the waters and the precipitated flocs were then evaluated. In columns employing Kaolin as a model for sediment removal, settling rates as high as 13.2 cm/min were observed using GE versus a control (suspensions with no treatment) settling at 0.5 cm/min. B. cereus tests displayed flocculation initiation up to 10 minutes faster than columns treated with calcium chloride (CaCl2) when using less than 10 ppm (GE) and 5 ppm (NE) of mucilage in addition to CaCl2. B. cereus removal rates between 95 and 98% have been observed in high concentration tests (> 108 cells/mL). Tests on E. coli flocculation differed slightly from those seen using B. cereus with control columns requiring 5 to 10 minutes longer to begin flocculation and mucilage treated columns displaying signs of flocculation much earlier. Mucilage is an ideal material for water purification and contaminant flocculation because it grows abundantly, is inexpensive and offers communities a sustainable technology.
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Pressure-Assisted Thermal Processing of Bacterial Spores: Influence of Selected Product and Packaging ParametersThammakulkrajang, Rarinthorn 28 September 2018 (has links)
No description available.
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Effects of high hydrostatic pressure processing on Bacillus cereus spores in fresh blue crab meat (Callinectes sapidus)Suklim, Kannapha 28 April 2006 (has links)
The Food and Drug Administration has recently expressed concern for the safety of seafood and seafood products. One of the concerns is the presence of Bacillus cereus in fresh blue crab meat. Bacillus cereus is a spore-forming pathogen whose spores survive the customary thermal treatments applied during cooking and pasteurization; therefore it could potentially present a health concern to consumers as the microorganism could increase to pathogenic levels.
The objectives of this study were to evaluate the effects of a post-processing method i.e. high hydrostatic pressure treatment on the quality of fresh crab meat and to evaluate the effectiveness of high pressures on the inactivation of B. cereus spores.
Fresh blue crab meat was pressurized at 300 and 550 MPa at 25° C for 5 min and stored at 4° C for 31 days to determine the pressurization effects on the microbiological, physical, and sensory quality of the meat. A pressure of 300 MPa caused a 1 log reduction in total aerobic plate count and a 3 day lag period, whereas 550 MPa inactivated 2 logs in total aerobic plate count with no evident lag phase. Physical and sensory qualities of pressurized crab meat were not statistically different from the untreated crab meat (P>0.05). A pressure of 300 MPa extended the shelf-life from 17 to over 24 days with the prevalence of Carnobacterium piscicola at the time of spoilage. Crab meat treated with 550 MPa was not rejected by sensory panels at day 31 and Enterococcus spp. was identified as the predominant microorganism.
High hydrostatic pressure (550 MPa at 40° C for 15 min) inactivated less than 1 log (0.66 log) of B. cereus spores inoculated in fresh crab meat. The meat essentially had a protective effect on pressure inactivation of the spores. During storage (31 days), surviving B. cereus was suppressed and outgrown by the other pressure resistant microflora at a storage temperature of 12° C. At 4° C, B. cereus could compete with the other pressure-resistant microflora and was isolated even at the end of the storage period (day 31); however, diarrheal toxin was not detected in any stored samples. / Ph. D.
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Einfluss verschiedener Probiotika (Bacillus cereus u. Saccharomyces cerevisiae) auf den in sacco Abbau und die Verdaulichkeit bei Schafen sowie die Mast- und Schlachtleistung von Jungbullen / Probiotika in der Wiederkäuerernährung / Effect of different probiotics (Bacillus cereus and Saccharomyces cerevisiae) on in sacco dry matter degradability in sheep, performance and slaughter of growing bulls / Probiotics in the nutrition of ruminantsGarza Cázares, José Fernando 12 July 2001 (has links)
No description available.
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Caracterização e potencial antioxidante do fruto da palma (Tacinga inamoena) e do mandacaru (Cereus jamacaru) / Characterization and palm fruit of the antioxidant potential (inamoena Tacinga) and mandacaru (Cereus jamacaru)Lima, Rydley Klapeyron Bezerra 24 February 2016 (has links)
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Previous issue date: 2016-02-24 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / There are several native and exotic fruit species of economic importance with potential for exploration in semi-arid region. The Tacinga inamoena and Cereus jamacaru are native plants of the Northeast region, which are distributed throughout most of the semi-arid, with great potential among the cacti. The study aimed to characterize the physical, chemical and antioxidant potential of the pulp and peel of fruits of palm and native mandacaru. The experiment was completely randomized, with 4 repetitions. The fruits of Tacinga inamoena and Cereus jamacaru were obtained from populations of plants that are found spontaneous and dispersed in the Caatinga, located in the cities of Angicos and Mossoró-RN. The fruits were harvested directly from the plant and taken to the Laboratory of Physiology and Technology of Fruit Postharvest of the Universidade Federal Rural do Semi-Árido (UFERSA) in Mossoró-RN, at which time it we determined: fresh fruit mass, fruit yield, longitudinal and transverse diameter, firmness, titratable acidity, relation SS/AT, pH, soluble solids, total and reducing sugars, vitamin C, yellow flavonoids, total carotenoids, total anthocyanins, polyphenols total extractable, betalains and antioxidant activity. The pulp of the fruits of Tacinga inamoena have high levels of phosphorus, potassium, calcium, magnesium and titratable acidity; they are also sources of vitamin C. The fruit pulp of Cereus jamacaru has high levels of calcium and potassium, low levels of acidity and high content of sugars and soluble solids/acidity ratio. The peel of fruits of Cereus jamacaru has high levels of calcium, magnesium and phosphorus. The pulp of fruits of Tacinga inamoena and the pulp and peel of the fruit Cereus jamacaru have significant levels of phenolic compounds and betalains; however, it has a low content of anthocyanins, carotenoids, flavonoids and antioxidant activity by ABTS method. With Basis on quality variables evaluated in this study, we say the fruits of Tacinga inamoena and Cereus jamacaru have the potential for commercialization in natura and industrialization / Diversas são as espécies frutíferas nativas e exóticas de importância econômica com potencial para exploração na região semiárida. A Tacinga inamoena e o Cereus jamacaru são plantas nativas da região Nordeste, que se encontram distribuída em quase todo o semiárido, com grande potencialidade entre as cactáceas. Assim, este trabalho teve como objetivo caracterizar quanto aos aspectos físicos, químicos e potencial antioxidante da polpa e casca dos frutos da palma e do mandacaru. O experimento adotado foi o inteiramente casualizado, com 4 repetições. Os frutos da Tacinga inamoena e Cereus jamacaru foram provenientes de populações de plantas que se encontram de forma espontânea e dispersa na Caatinga, localizados nos municípios de Angicos e Mossoró-RN. Os frutos foram colhidos diretamente na planta e conduzidos ao Laboratório de Fisiologia e Tecnologia Pós-colheita de Frutos da Universidade Federal Rural do Semiárido (UFERSA), em Mossoró-RN, ocasião em que se determinou: massa fresca, rendimento, diâmetro longitudinal e transversal, firmeza, acidez titulável (AT), sólidos solúveis (SS), relação SS/AT, pH, açúcares totais e redutores, vitamina C, flavonóides amarelos, carotenóides, antocianinas, polifenóis, betalaínas e atividade antioxidante. A polpa dos frutos da Tacinga inamoena tem elevados teores de fósforo, potássio, cálcio, magnésio e acidez titulável; também são fontes de vitamina C. A polpa dos frutos do Cereus jamacaru tem elevados teores de cálcio e potássio, baixo teores de acidez e elevado conteúdo de açúcares e da relação sólidos solúveis/acidez. A casca dos frutos do Cereus jamacaru tem elevados teores de cálcio, magnésio e fósforo. A polpa dos frutos de Tacinga inamoena e a polpa e casca do fruto de Cereus jamacaru tem significativo teores de compostos fenólicos e betalaínas; entretanto, tem baixo conteúdo de antocianinas, carotenoides, flavonoides e atividade antioxidante pelo método ABTS. Com base nas variáveis de qualidade avaliada no presente estudo, os frutos da Tacinga inamoena e do Cereus jamacaru tem potencial para comercialização na forma in natura e para industrialização
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Caracterização e potencial antioxidante do fruto da palma (Tacinga inamoena) e do mandacaru (Cereus jamacaru) / Characterization and palm fruit of the antioxidant potential (inamoena Tacinga) and mandacaru (Cereus jamacaru)Lima, Rydley Klapeyron Bezerra 24 February 2016 (has links)
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Previous issue date: 2016-02-24 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / There are several native and exotic fruit species of economic importance with potential for exploration in semi-arid region. The Tacinga inamoena and Cereus jamacaru are native plants of the Northeast region, which are distributed throughout most of the semi-arid, with great potential among the cacti. The study aimed to characterize the physical, chemical and antioxidant potential of the pulp and peel of fruits of palm and native mandacaru. The experiment was completely randomized, with 4 repetitions. The fruits of Tacinga inamoena and Cereus jamacaru were obtained from populations of plants that are found spontaneous and dispersed in the Caatinga, located in the cities of Angicos and Mossoró-RN. The fruits were harvested directly from the plant and taken to the Laboratory of Physiology and Technology of Fruit Postharvest of the Universidade Federal Rural do Semi-Árido (UFERSA) in Mossoró-RN, at which time it we determined: fresh fruit mass, fruit yield, longitudinal and transverse diameter, firmness, titratable acidity, relation SS/AT, pH, soluble solids, total and reducing sugars, vitamin C, yellow flavonoids, total carotenoids, total anthocyanins, polyphenols total extractable, betalains and antioxidant activity. The pulp of the fruits of Tacinga inamoena have high levels of phosphorus, potassium, calcium, magnesium and titratable acidity; they are also sources of vitamin C. The fruit pulp of Cereus jamacaru has high levels of calcium and potassium, low levels of acidity and high content of sugars and soluble solids/acidity ratio. The peel of fruits of Cereus jamacaru has high levels of calcium, magnesium and phosphorus. The pulp of fruits of Tacinga inamoena and the pulp and peel of the fruit Cereus jamacaru have significant levels of phenolic compounds and betalains; however, it has a low content of anthocyanins, carotenoids, flavonoids and antioxidant activity by ABTS method. With Basis on quality variables evaluated in this study, we say the fruits of Tacinga inamoena and Cereus jamacaru have the potential for commercialization in natura and industrialization / Diversas são as espécies frutíferas nativas e exóticas de importância econômica com potencial para exploração na região semiárida. A Tacinga inamoena e o Cereus jamacaru são plantas nativas da região Nordeste, que se encontram distribuída em quase todo o semiárido, com grande potencialidade entre as cactáceas. Assim, este trabalho teve como objetivo caracterizar quanto aos aspectos físicos, químicos e potencial antioxidante da polpa e casca dos frutos da palma e do mandacaru. O experimento adotado foi o inteiramente casualizado, com 4 repetições. Os frutos da Tacinga inamoena e Cereus jamacaru foram provenientes de populações de plantas que se encontram de forma espontânea e dispersa na Caatinga, localizados nos municípios de Angicos e Mossoró-RN. Os frutos foram colhidos diretamente na planta e conduzidos ao Laboratório de Fisiologia e Tecnologia Pós-colheita de Frutos da Universidade Federal Rural do Semiárido (UFERSA), em Mossoró-RN, ocasião em que se determinou: massa fresca, rendimento, diâmetro longitudinal e transversal, firmeza, acidez titulável (AT), sólidos solúveis (SS), relação SS/AT, pH, açúcares totais e redutores, vitamina C, flavonóides amarelos, carotenóides, antocianinas, polifenóis, betalaínas e atividade antioxidante. A polpa dos frutos da Tacinga inamoena tem elevados teores de fósforo, potássio, cálcio, magnésio e acidez titulável; também são fontes de vitamina C. A polpa dos frutos do Cereus jamacaru tem elevados teores de cálcio e potássio, baixo teores de acidez e elevado conteúdo de açúcares e da relação sólidos solúveis/acidez. A casca dos frutos do Cereus jamacaru tem elevados teores de cálcio, magnésio e fósforo. A polpa dos frutos de Tacinga inamoena e a polpa e casca do fruto de Cereus jamacaru tem significativo teores de compostos fenólicos e betalaínas; entretanto, tem baixo conteúdo de antocianinas, carotenoides, flavonoides e atividade antioxidante pelo método ABTS. Com base nas variáveis de qualidade avaliada no presente estudo, os frutos da Tacinga inamoena e do Cereus jamacaru tem potencial para comercialização na forma in natura e para industrialização
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