Many species from economically important genera remain rooting recalcitrant, prohibiting the commercialisation of many species in forestry and horticulture, and hindering genetic improvement by conventional breeding or recombinant DNA technology, where vegetative propagation is often used to preserve the genetic fidelity of elite progeny. Two cherry species (Prunus avium and P. padus) were used as models in this study to investigate the physiological and genetic manipulation of adventitious rooting. Mature trees are typically more difficult to propagate vegetatively than their juvenile counterparts. For some trees, micropropagation can circumvent certain effects of ageing and maturation, restoring shoot vigour and rooting, but the mechanism(s) involved have not been elucidated. During micropropagation, subculture interval was found not to be the predominant factor promoting the 'apparent rejuvenation' of mature P. avium tissue. 'Apparently rejuvenated' ex vitro and hedged (putatively) mature P. avium trees were treated with gibberellins predicted to have a range of structural related activities. GA, improved the rooting of cuttings from hedged (putatively) mature cherry, but not from ex vitro trees. Methodology to regenerate adventitious shoots from P. avium leaf explants was developed, (putative) transgenic P. padus plants were produced by an Agrobacterium tumefaciens mediated strategy. Auxin redistribution in planta is postulated to require a component of active transport; inhibition of the predominantly basipetal transport has profound effects on rooting. The putative function of the Arabidopsis thaliana AtAUX1 gene is that of a cellular auxin influx carrier, possibly, as described by the chemiosmotic hypothesis. This thesis examined the hypothesis that transformation with the AtAUX1 gene would enhance the delivery of the root-inducing signal to improve rooting of P. padus, a species which is rooting recalcitrant and more or less obligate on exogenous auxin for this process. However, all six, constitutively expressed, Cauliflower Mosaic Virus 35S promoter driven, 35S::AtAUX1, transgenic shoot lines had reduced rooting.

Made available in DSpace on 2014-06-11T19:26:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-05-26Bitstream added on 2014-06-13T20:55:00Z : No. of bitstreams: 1 adriano_e_me_botfca.pdf: 660248 bytes, checksum: b78236767080a845ff7e3cae1f588e01 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A aceroleira é uma planta de clima tropical que produz frutos ricos em vitamina C. Esta fruta é comercializada principalmente na forma de polpa congelada e fruto in natura. O objetivo deste estudo foi avaliar a fenologia, produção e qualidade dos frutos da aceroleira cv. Olivier. Para tanto foi implantado um experimento em pomar comercial no município de Junqueirópolis-SP, no ano agrícola de 2009/2010. Foram avaliados dois ciclos reprodutivos: setembro e janeiro. Para avaliação fenológica, em cada época foram marcados 40 botões florais em 10 plantas, totalizando 400 botões. O ciclo foi dividido em estádios fenológicos, sendo avaliada a duração dos estádios, taxa de frutificação e crescimento dos frutos. Na avaliação da produção os frutos maduros foram colhidos e pesados. Para análise qualitativa foram coletadas amostras de frutos e avaliados o peso médio de fruto, cor de fruto e as características químicas da polpa: teor de sólidos solúveis, pH, acidez titulável, açúcares redutores, ‘ratio’ e teor de ácido ascórbico. Os resultados obtidos permitiram concluir que a duração dos estádios é influenciada pela temperatura ambiente, sendo mais curto em condições de altas temperaturas. A elevada incidência de precipitações interfere negativamente na fixação dos frutos da aceroleira, prejudicando a produção. Também pode-se observar que o maior índice de abortamento ocorre no estádio de perda de pétala. Com relação ao desenvolvimento dos frutos, apesar da diferença na velocidade de crescimento e tamanho dos frutos, em ambas as avaliações pode-se observar que houve um mais acentuado nos primeiros 8 dias, seguindo depois um padrão linear. Para os dados de produção e qualidade dos frutos pode-se concluir que houve influência da época... / The barbados cherry tree is a tropical plant that produces fruits rich in vitamin C. This fruit is marketed mainly in the form of frozen pulp and fresh fruit. The present study aimed to evaluate the reproductive phenology, production and fruit quality of barbados cherry trees cv. Olivier at different times. Thus an experiment was deployed in the city of orchard Junqueirópolis-SP, in the agricultural year 2009/2010. Were evaluated two reproductive cycles: September and January. To evaluated of phenology in each times were marked 40 buds on 10 plants, totaling 400 buttons. The cycle was divided at different phenological stages, were evaluated for the duration of the stage, rate of fruit and fruit growth. Measuring the output ripe fruits were harvested and weighed. For qualitative analysis of fruit samples were collected and evaluated the average fruit weight, fruit color and chemical characteristics of pulp: soluble solids, pH, acidity, reducing sugars, 'ratio' and ascorbic acid. The results showed that the duration of the stages is influenced by temperature, being shorter in high temperature conditions. The high incidence of rainfall impacts negatively on fruit set of barbados cherry trees, hurting production. One can also observe that the highest rate of abortion occurs the stage of petal loss. In relation to fruit development, despite the difference in growth rate and fruit size in both assessments can be observed that there was a more pronounced during the first 8 days and then follows a linear pattern. For data production and fruit quality can be concluded that there was influence of the time, with production in September was higher, but the fruits harvested in January showed a higher average fruit weight. There were differences in pulp quality parameters of two times, with the fruits of the harvest in September... (Complete abstract click electronic access below)

Orientador: Sarita Leonel / Banca: Aloísio Costa Sampaio / Banca: Simone Rodrigues da Silva / Resumo: A aceroleira é uma planta de clima tropical que produz frutos ricos em vitamina C. Esta fruta é comercializada principalmente na forma de polpa congelada e fruto in natura. O objetivo deste estudo foi avaliar a fenologia, produção e qualidade dos frutos da aceroleira cv. Olivier. Para tanto foi implantado um experimento em pomar comercial no município de Junqueirópolis-SP, no ano agrícola de 2009/2010. Foram avaliados dois ciclos reprodutivos: setembro e janeiro. Para avaliação fenológica, em cada época foram marcados 40 botões florais em 10 plantas, totalizando 400 botões. O ciclo foi dividido em estádios fenológicos, sendo avaliada a duração dos estádios, taxa de frutificação e crescimento dos frutos. Na avaliação da produção os frutos maduros foram colhidos e pesados. Para análise qualitativa foram coletadas amostras de frutos e avaliados o peso médio de fruto, cor de fruto e as características químicas da polpa: teor de sólidos solúveis, pH, acidez titulável, açúcares redutores, 'ratio' e teor de ácido ascórbico. Os resultados obtidos permitiram concluir que a duração dos estádios é influenciada pela temperatura ambiente, sendo mais curto em condições de altas temperaturas. A elevada incidência de precipitações interfere negativamente na fixação dos frutos da aceroleira, prejudicando a produção. Também pode-se observar que o maior índice de abortamento ocorre no estádio de perda de pétala. Com relação ao desenvolvimento dos frutos, apesar da diferença na velocidade de crescimento e tamanho dos frutos, em ambas as avaliações pode-se observar que houve um mais acentuado nos primeiros 8 dias, seguindo depois um padrão linear. Para os dados de produção e qualidade dos frutos pode-se concluir que houve influência da época... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The barbados cherry tree is a tropical plant that produces fruits rich in vitamin C. This fruit is marketed mainly in the form of frozen pulp and fresh fruit. The present study aimed to evaluate the reproductive phenology, production and fruit quality of barbados cherry trees cv. Olivier at different times. Thus an experiment was deployed in the city of orchard Junqueirópolis-SP, in the agricultural year 2009/2010. Were evaluated two reproductive cycles: September and January. To evaluated of phenology in each times were marked 40 buds on 10 plants, totaling 400 buttons. The cycle was divided at different phenological stages, were evaluated for the duration of the stage, rate of fruit and fruit growth. Measuring the output ripe fruits were harvested and weighed. For qualitative analysis of fruit samples were collected and evaluated the average fruit weight, fruit color and chemical characteristics of pulp: soluble solids, pH, acidity, reducing sugars, 'ratio' and ascorbic acid. The results showed that the duration of the stages is influenced by temperature, being shorter in high temperature conditions. The high incidence of rainfall impacts negatively on fruit set of barbados cherry trees, hurting production. One can also observe that the highest rate of abortion occurs the stage of petal loss. In relation to fruit development, despite the difference in growth rate and fruit size in both assessments can be observed that there was a more pronounced during the first 8 days and then follows a linear pattern. For data production and fruit quality can be concluded that there was influence of the time, with production in September was higher, but the fruits harvested in January showed a higher average fruit weight. There were differences in pulp quality parameters of two times, with the fruits of the harvest in September... (Complete abstract click electronic access below) / Mestre

京都大学 / 新制・論文博士 / 博士(農学) / 乙第13547号 / 論農博第2912号 / 新制||農||1101(附属図書館) / 学位論文||R5||N5428(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 大村 和香子, 教授 井鷺 裕司, 教授 今井 友也 / 学位規則第4条第2項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

This master's thesis explores the potential for collaboration among diverse actors on the island of Gotland to achieve environmental sustainability. The study employs the concept of biomimicry, utilising selected natural phenomena as a source of inspiration. A theoretical framework and qualitative research methodology are employed to guide the process, with the aim of developing a strategy to enhance the collaborative state for environmental sustainability on Gotland. Drawing on principles derived from nature, the study investigates how biomimicry can provide solutions and inspire collaborative efforts for environmental sustainability. Central to the research is the exploration of trust-building mechanisms among diverse actors. Recognising that trust is a fundamental ingredient for effective collaboration, the study examines strategies that can foster trust within the context of environmental sustainability. By identifying barriers and enablers of trust, the thesis proposes actionable recommendations to enhance the trust-building process on Gotland, thereby fostering a conducive environment for collaboration. The proposed strategy aims to create a collaborative framework that fosters long-term partnerships, harnessing the diverse expertise and resources of each actor to address the multifaceted challenges of environmental sustainability on Gotland. In conclusion, this master's thesis underscores the value of applying biomimicry principles to foster collaboration for environmental sustainability on Gotland.

In her doctoral thesis the author would like to present the play Yoshitsune and the Thousand Cherry trees (Yoshitsune senbonzakura) as a unique piece of Japanese drama which has a great complexity and an outstanding place within the corpus of Japanese literature. Even if the play can be compared to Chushingura mono in its importance, Yoshitsune senbonzakura has not been widely translated to western languages. There are two exceptions, however: the English translation (Jones, Jr. 1993) and the German translation (Klopfenstein 1982). While comparing the two translations and using the most full original texts available (as preserved in Takeda Izumo and Namiki Sosuke Joruri Collection and Yuda Yoshio's Bunraku Joruri Collection), the author's goal is to present the play to Czech readers and its interpretation to Czech scholars. As for the flow and structure of the interpretation of Yoshitsune senbonzakura the author begins with the historical background of the legend of Minamoto Yoshitsune, capturing the life of Yoshitsune from the time of Heiji rebellion (1159) when he was born to his death in 1189. Next the author concentrates on the legend itself as it evolved within the course of Japanese literature. With a shift from the court literature of Heian period towards the battlefield stories and...

In her doctoral thesis the author would like to present the play Yoshitsune and the Thousand Cherry trees (Yoshitsune senbonzakura) as a unique piece of Japanese drama which has a great complexity and an outstanding place within the corpus of Japanese literature. Even if the play can be compared to Chushingura mono in its importance, Yoshitsune senbonzakura has not been widely translated to western languages. There are two exceptions, however: the English translation (Jones, Jr. 1993) and the German translation (Klopfenstein 1982). While comparing the two translations and using the most full original texts available (as preserved in Takeda Izumo and Namiki Sosuke Joruri Collection and Yuda Yoshio's Bunraku Joruri Collection), the author's goal is to present the play to Czech readers and its interpretation to Czech scholars. As for the flow and structure of the interpretation of Yoshitsune senbonzakura the author begins with an analysis of the historical background of the legend of Minamoto Yoshitsune, capturing the life of Yoshitsune from the time of Heiji rebellion (1159) when he was born to his death in 1189. Next the author concentrates on the legend itself as it evolved within the course of Japanese literature. With a shift from the court literature of Heian period towards the battlefield...