Unidades climáticas são determinadas áreas da superfície terrestre que apresentam características climáticas relativamente homogêneas ao longo do tempo e do espaço. Sua existência, a priori, depende da relação única entre a superfície terrestre e a dinâmica atmosférica, quando a primeira exerce controle sobre a segunda, influenciando tais características. Essas características climáticas, sob a forma da variação temporal da temperatura, umidade, chuvas, entre outros, são espacialmente melhor percebidas de acordo com a escala de trabalho da pesquisa adotada e podem realçar a existência de ambientes diferenciados (daqueles conhecidos em escalas mais generalistas) - os climas locais e os topoclimas, que são a base climatológica mais complexa dos planos de ordenação territorial. O município de Ilhabela reflete as transformações socioeconômicas e ambientais que vem ocorrendo na região do Litoral Norte do estado de São Paulo, essencialmente no período posterior a 1970, com a inauguração da Rodovia Rio-Santos (BR101/SP55), que pressupõem, por conta do aporte migratório, transformações nas paisagens locais e regionais. Entretanto, a Ilha de São Sebastião (a maior e sede do município) resguarda 75% de seu território sob a forma de unidade de conservação Parque Estadual de Ilhabela (PEIb). Desta forma, a essência do estudo aqui proposto é a identificação das unidades climáticas naturais da ilha, por meio da análise da variação de temperatura, umidade relativa e precipitações identificadas em diferentes localidades deste ambiente ilhéu e avaliar possíveis influências da urbanização sobre este processo. O método utilizado nesta pesquisa está assentado na instalação de equipamentos sensores da temperatura do ar, umidade relativa e da precipitação líquida como os termo-higrômetros (eletrônicos) e os pluviômetros (totalizadores e registradores, manuais e eletrônicos, respectivamente), dentro do período de um ano. A análise dos dados considerou aspectos geográficos e climatológicos de ordem local, assim como aqueles de ordem regional (meteorológicos sinóticos) resultando em informações quantitativas e qualitativas, que sob a luz da análise rítmica e da heurística possibilitaram sua análise crítica. Os resultados, quando observados individualmente, representam a situação geoclimática dos sítios de instalação da instrumentação meteorológica; por outro lado, a variabilidade espacial dos atributos em sua relação com os sistemas atmosféricos atuantes, considerados então como representativos dos meso e topoclimas, permite a identificação, individualização e definição de unidades climáticas da ilha no período da coleta de dados. Foram então definidos 12 ambientes topoclimaticamente diferenciados com a clara percepção de heterogeneidade entre si, organizados sob 5 mesoclimas conforme a insolação, os fluxos de ar predominantes, a orientação geral das vertentes e das bacias hidrográficas e a variação dos atributos do clima. O setor norte: quente e pouco chuvoso. A vertente leste: mais ensolarada e úmida. O sul sudoeste: barlavento dos sistemas frontais. O setor central: urbano é sujeito a atividade convectiva intensa. / Climate units are certain areas of the Earth\'s surface that have relatively homogeneous climatic characteristics over time and space. Its existence, in principle, depends on the unique relationship between the ground surface and the atmospheric dynamics, where the first exercises control over the second influencing such features. These climatic characteristics in the form of temporal variation of temperature, humidity, rainfall, among others, are spatially better perceived according to the adopted research work schedule and can highlight the existence of different environments (those known in more general scales) - local climates and topoclimas, which are the most complex climatological basis of territorial development plans. The municipality of Ilhabela reflects the socioeconomic and environmental changes that have occurred in the North Coast region of the state of São Paulo, mainly in the period to 1970, with the inauguration of the Rio-Santos highway (BR101 / SP55), which require, due to the migratory contribution, changes in local and regional landscapes. However, Sao Sebastiao Island (the largest and county seat) protects 75% of its territory in the form of conservation unit - Ilhabela State Park (PEIb). Thus, the essence of the herein proposed study is to identify the natural climate units of the island, through the analysis of the variation of temperature, relative humidity and precipitation identified in different locations of the islet environment and to evaluate the possible influence of urbanization on this process. The method used in this research is seated in the installation of sensor equipment of the air temperature, relative humidity and liquid precipitation as the thermo-hygrometers (electronic) and rain gauges (totalizers and registers, manual and electronic, respectively) within the period of one year. Data analysis considered geographical and climatological aspects of local order, as well as those of regional order (synoptic weather) resulting in quantitative and qualitative information, which in the light of rhythmic analysis and heuristics enabled critical analysis. The results, when viewed individually, represent the geoclimática situation of installation sites of meteorological instrumentation; on the other hand, the spatial variability of the attributes in its relation to the active atmospheric systems, considered then as representative of meso and topoclimas allows the identification, individualization and definition of climate units of the island in the period of data collection. They were then set 12 topoclimaticamente different environments with a clear perception of heterogeneity among themselves, organized under 5 mesoclimas as heatstroke, prevailing air flows, the general orientation of the slopes and river basins and the change of climate attributes. The northern sector: hot and slightly rainy. The east side: more sunny and humid. The south southwest: windward of frontal system. The central sector: Urban is subject to intense convective activity.

No description available.

The pastoral problem being addressed has to do with the culture of consumerism and individualism that have influenced the culture of the Church. Within Western society, the “ism” culture, consisting of factors such as: hedonism, consumerism, materialism, secularism, relativism and individualism, has become very prominent. Christians often operate no differently than non-Christians, and this is problematic since Christians are called to be salt for the earth and light for the world (Matthew 5:13-16), a people set apart. In John 15:19, we are told that we were not made to be of this world, so we need to stop being influenced by the culture and instead allow the beauty of the Christian faith to influence the world. What is presented here is not just a hope to overcome consumerism and individualism, but an entire revamping of what it means to be Christian. It suggests the need for a culture change, which if Christians and church leadership focus on, has the power to solve other pastoral and ethical issues as well. Such a cultural change will lead to vibrancy among believers and will attract more people to the faith, as well.

No description available.

<p>The need to secure software systems is more important than ever. However, while a lot of work exists to design and implement secure systems, a fundamental weakness remains. Instead of implementing software with least privilege policies, developers create monolithic systems that allow any instruction near universal memory access. This dissertation attempts to rectify this fundamental weakness to software design through three different contributions.</p> <p>First, I address the monolithic software design problem by proposing and evaluating a novel compartmentalization enforcement mechanism called Hardware-Assisted Kernel Compartmentalization (HAKC). HAKC is capable of enforcing an arbitrary compartmentalization policy using features of the ARMv9 ISA, without the need of any extra virtualization or trusted software layer. I then introduce a method of determining an optimal compartmentalization policy based on user performance and security constraints called FlexC, which is tested using HAKC as the enforcement mechanism. The end result is a hardened, com-partmentalized kernel, customized to a user’s needs, which enforces a least privilege policy that minimizes overhead. Finally, as an avenue for further compartmentalization policy generation, I introduce a novel program analysis framework called IOVec Function Identifier (IOVFI), which foregoes the use of language processing and model learning, but instead uses program state changes as a unique function fingerprint. I show that IOVFI is a more stable and accurate function identifier than the state-of-the-art, even in the presence of differing compilation environments, purposeful obfuscations, and even architecture changes.</p>

Follicular dendritic cells (FDCs) reside in the lymphoid follicles of the secondary lymphoid tissues (sLTs). Following the infection of an individual with human immunodeficiency virus type 1 (HIV-1), viral particles are trapped in massive quantities on the surfaces of FDCs. HIV-1 viral compartments are cell types or tissues between which there is a restriction of virus flow. Compartmentalization of HIV-1 creates numerous sites within the body in which the virus can undergo independent evolution, giving rise to a more diverse total viral population. Given the sessile nature of the FDC, I hypothesized that contrary to common assumptions, FDC-trapped HIV-1 is compartmentalized between different sLTs. Furthermore, given that FDC-trapped HIV-1 represents the major source of virus in the host, I postulated that this compartmentalization would likely impact the diversity of HIV-1 associated with the sLTs. I isolated FDCs, macrophages, and T cells from various sLTs, and sequenced cloned HIV-1 associated with these three cell populations. I subjected the resulting DNA and cDNA sequence data to phylogenetic and other statistical analyses. In support of my hypothesis, I demonstrate that both HIV-1 gp120 and pol sequences cloned from FDCs are compartmentalized between different sLTs. This compartmentalization is even apparent between lymph nodes taken from the same lymph node chain. One of the apparent effects of this compartmentalization is to significantly increase the viral genetic diversity in multiple sLTs when compared with diversity in a single sLT. It also appears that the selective pressures on HIV-1 differ among the sLTs. In addition, when proviruses isolated from macrophages from different sLTs were compared, it was also evident that there is compartmentalization of HIV-1 associated with this cell type as well. Finally, I demonstrate that HIV-1 isolated from an unfractionated population of cells from a single sLT, may be an inadequate representation of the total viral population in that sLT. Taken together, my data suggest that the nature of HIV-1 in the sLTs may be more complex than currently appreciated.

No description available.

Using an integrated dataset comprising outcrop, core, GPR and LiDAR data, this study targets a high-quality outcrop "window" of the upper Cretaceous Blackhawk Formation in the eastern Wasatch Plateau in central Utah, spanning a fairly large spatial (~30 km2 area comprising eight contiguous, and vertical cliff faces) and temporal (~4 my) range. This research provides field-validation and -calibration of a wider range of fluvial heterogeneity: 1) large-scale heterogeneity (10’s of m vertically and 100’s of m laterally), 2) intermediate-scale heterogeneity (1’s of m vertically and 10’s of m laterally), and 3) small-scale heterogeneity (10’s of cm vertically and 1’s of m laterally). These sandbody- to facies-scale heterogeneities generate potential for stratigraphic compartmentalization for analogous fluvial reservoirs and prospects. Moreover, these results specifically constitute an outcrop analog to the producing tight-gas fluvial reservoirs of the adjacent hydrocarbon-prolific Uinta and Piceance Basins of Utah and Colorado, including the giant Jonah Field of Wyoming. 3D virtual outcrop model generated from LiDAR-integration has helped in avulsion-scale (~1's-10's kyr) to basin-fill scale (~100's kyr-1's myr) fluvial sandbody organization analysis down to channel-storey level. This high-resolution analysis has brought several intriguing insights. single-storey sandbodies are preferentially attendant to clustering organization, whereas multi-lateral sandbodies (i.e. channel-belt) show compensational-prone behavior. Sandbody organization is broadly compensational for the lower Blackhawk Formation, where the floodplain facies diversity is the highest. In contrast, floodplain diversity decreases stratigraphically upward such that the upper Blackhawk Formation shows the least heterogeneous floodplain with clustering-prone sandbody organization. In the quest of differentiating autogenic from allogenic signal in dynamic systems where their interplay is complexly intertwined, this study presents two incised-valley examples, where resultant fluvial organization has been interpreted, contrary to conventional wisdom, to be preferentially modulated by a dominant controlling mechanism of autogenic forcing. In filling these incised valley deposits, each of which is up to ~15-20 m thick, the dominating behavior of substrate coal compaction as an autogenic mechanism supplanted allogenic forcing (i.e. sea-level fluctuation).

La biologie des réactions redox est particulièrement difficile à étudier de par la compartimentation spatiale mais aussi cinétique des différents systèmes redox cellulaires. Les biosenseurs codés génétiquement, incluant la «redox-sensitive Yellow Fluorescent Protein» (rxYFP) sont une manière de contourner les limitations des méthodes conventionnelles de mesure du couple glutathion/glutathion disulfure (GSH/GSSG). Cette étude présente l’utilisation des biosenseurs rxYFP pour analyser les états redox dans des différents compartiments cellulaires, et leur dynamique en réponse au stress dans les cellules humaines. La rxYFP exprimée soit dans le cytosol, le noyau ou la matrice mitochondriale de cellules HeLa s’est révélée sensible aux changements de l’état redox intracellulaire provoqué par des traitements aussi bien réducteurs qu’oxydants. La rxYFP est capable de détecter des différences de l’état redox, entre les compartiments, mais aussi entre différentes lignées cellulaires. Les senseurs exprimés dans des kératinocytes humain de l’épiderme HEK001 ont réagi au stress induit par les UVA, de façon dose-dépendante, mais pas au stress induit par les UVB. De plus, ces senseurs ont pu détecter les changements redox induits par des faibles doses (30 µM) ainsi que par des doses modérées (100 µM) de peroxyde d’hydrogène (H2O2), de façon dynamique et spécifique des compartiments cellulaires. La rxYFP exprimé dans la matrice mitochondriale a montré une vitesse d’oxydation plus élevée que les senseurs rxYFP exprimés dans le cytosol ou le noyau, ce qui est attribuable à un pH local plus basique. De plus, la déplétion en GSH provoquée par un traitement au buthionine sulphoximine (BSO) a affecté spécifiquement le potentiel redox mitochondrial mais pas cytosolique ni nucléaire. Ces observations soutiennent l’idée que l’état redox du GSH mitochondrial est maintenu et régulé de façon indépendante par rapport à celui du cytosol ou du noyau. Nous avons également montré que dans les cellules humaines, les sondes rxYFP réagissent de façon prédominante avec le GSH/GSSG, puisque la déplétion en GSH ralentit la vitesse d’oxydation de la rxYFP en réponse à un traitement par H2O2. De plus, grâce à l’utilisation des sondes rxYFP et à l’analyse de l’état redox des antioxydants cellulaires, nous démontrons que l’oxydation des thiols se produit après l’activation des caspases au cours de l’apoptose induite par TRAIL. L’ensemble de nos données montrent la robustesse des senseurs rxYFP pour la mesure des changements d’état redox dans les cellules humaines. En complément d’autres senseurs redox ainsi que des méthodes conventionnelles de mesure des états redox, les senseurs rxYFP ciblés aux différents compartiments cellulaires sont un nouvel outil pour étudier l’homéostasie redox dans les cellules de mammifères, et permettent l’étude de l’état redox du glutathion et de la dynamique des changements redox avec une grande précision. / The kinetic and spatial separation of redox systems renders redox biology studies a particularly challenging field. Genetically encoded biosensors including the glutathione-specific redox-sensitive yellow fluorescent protein (rxYFP) may provide an alternative way to overcome the limitations of conventional glutathione/glutathione disulfide (GSH/GSSG) redox measurements. This study describes the use of rxYFP sensors for investigating compartment-specific steady redox states and their dynamics in response to stress in human cells. RxYFP expressed either in the cytosol, nucleus or mitochondrial matrix of HeLa cells was responsive to the intracellular redox state changes induced by reducing as well as oxidizing agents. Compartment-targeted rxYFP sensors were able to detect different steady state redox conditions between the cytosol, nucleus and mitochondrial matrix as well as between the cell lines. These sensors expressed in human epidermal keratinocytes HEK001 responded to stress induced by UVA radiation in a dose-dependent manner but not to UVB radiation. Furthermore, rxYFP sensors were able to sense dynamic and compartment-specific redox changes caused by low dose (30 µM) and moderate dose (100 M) hydrogen peroxide (H2O2). Mitochondrial matrix-targeted rxYFP displayed a greater dynamics of oxidation in response to a H2O2 challenge than the cytosol- and nucleus-targeted sensors, largely due to a more alkaline local pH environment. Similarly, the depletion of glutathione induced by buthionine sulphoximine (BSO) affected selectively mitochondrial redox potential without inducing changes in cytosol and nucleus. Furthermore, using rxYFP probes and cellular antioxidants redox state analysis, we show that oxidation of thiols occurs after activation of caspases during TRAIL-induced apoptosis. These observations support the view that mitochondrial glutathione redox state is maintained and regulated independently from that of the cytosol and nucleus. We also showed that in human cells the rxYFP probes react predominantly with glutathione since the glutathione depletion slows down the dynamics of rxYFP oxidation in response to H2O2. Taken together, our data show the robustness of the rxYFP sensors to measure compartmental redox changes in human cells. Complementary to existing redox sensors and conventional redox measurements, compartment-targeted rxYFP sensors provide a novel tool for examining mammalian cell redox homeostasis, permitting high resolution readout of steady glutathione state and dynamics of redox changes.

The United States is experiencing a severe shortage of Emergency Medical Service (EMS) paramedics. The job outlook for paramedics (EMT-P) for the years 2012-2022 is predicted to increase by 23%-33%, which is much faster than the 4% average increase of other first-response professions. The average tenure of paramedics is less than 4 years. There is a significant gap in the literature concerning paramedic personality traits and tenure. The primary objective of the current study is to provide empirical data on the personality traits possessed by long-term paramedics (5+ years), and compare them to those with shorter tenure (< 5 years). Using Allport's Trait Theory, I predicted that personality would affect paramedic longevity. The 6 personality traits tested were warmth, reasoning, emotional stability, liveliness, social boldness and openness to change. A socio-demographic questionnaire, determined the length of their EMS career, while the 16PF® Assessment, tested their personality traits. Using t tests, Mann-Whitney U tests, and a set of regression analyses, data were examined to determine if length of career and personality traits predicted paramedic tenure. The research sample consisted of long term paramedics and former paramedics. The results showed that of the 6 personality traits, only warmth was a significant predictor of paramedic tenure. A logistic regression showed for every additional point in warmth, the odds of leaving EMS prior to 5 years increased by a factor of 2.77. This study provides support for positive social change by helping EMS to learn how to increase recruitment and tenure. It also helps by advising EMS agencies to attend to the mental and emotional health of their paramedics by being aware of the level of their warmth personality trait.