Made available in DSpace on 2014-06-11T19:33:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-04-28Bitstream added on 2014-06-13T21:06:43Z : No. of bitstreams: 1 carlin_sd_dr_jabo.pdf: 560339 bytes, checksum: 8b2e1669f83b62325f0519f3baf373d4 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / No presente trabalho foi verificado o ajuste osmótico no crescimento inicial de cana-de-açúcar, cv. IAC91-5155, sob efeito da interação dos estresses hídrico e ácido no solo. Para tanto, foram quantificados os teores de três solutos compatíveis, prolina, glicina betaína e trealose. Também foi estudada a atividade da nitrato redutase e os teores de clorofilas. O estudo de todas estas variáveis teve por objetivo propor a utilização destes compostos como indicadores fisiológicos de aclimatação da cana-deaçúcar sob efeito da interação de ambos os estresses. O experimento foi realizado em casa de vegetação com 29,7±4,3oC e 75±10 UR%. Foram utilizados três tratamentos de disponibilidade hídrica (capacidade de campo, CC): controle (70%), estresse moderado (55%) e estresse severo (40%) e três tratamentos de acidez no solo (saturação por bases, V%): controle (55%), média acidez (33%) e alta acidez (23%). O delineamento experimental utilizado foi o de blocos casualizados em esquema fatorial 3x3, com quatro repetições. Após 60 dias sob estresses foram determinados os teores dos solutos compatíveis, a atividade da nitrato redutase e os teores das clorofilas. Os resultados mostram que houve acúmulo dos três solutos compatíveis evidenciando que ocorreu ajuste osmótico. Prolina e glicina betaína são indicadores fisiológicos do efeito da interação de ambos os estresses enquanto trealose é indicadora apenas para o estresse hídrico. Também, a atividade da nitrato redutase pode ser utilizada como indicador do estresse hídrico e os teores das clorofilas a, b e total para os estresses hídrico ou ácido. A massa de matéria seca da parte aérea, o número de folhas e a área foliar das plantas jovens de cana-de-açúcar, ajustadas osmoticamente, são reduzidos pelos estresses. Os efeitos interativos de ambos... / In this present work was verified the osmotic adjustment in the initial plant growth sugarcane cv. IAC91-5155 under interaction effect of water and acid stress in the soil. For this, were quantified three compatible solutes content, as follows: proline, glycine betaine and trehalose. It was verified also the nitrate reductase activity as well the chlorophyll content. The study of all parameters aimed to propose the use components as physiological indicators to acclimation of sugarcane plants under interaction the both stress. The experiment was carried out in a greenhouse under 29.7±4.3oC and 75±10%. Three water availability treatments (Field Capacity – FC%): control (70%), moderate stress (55%), and extreme stress (40%) and three soil acidification treatment (Base Saturation – BS%): control (55%), average acidity (33%), and high acidity (23%) were used. The experimental design used was the randomized blocks in a 3x3 factorial scheme and four replicates. After sixty days under stress were determined compatible solutes, nitrate reductase and chlorophyll content. Our results showed that there was the accumulation of three compatible solutes exhibiting osmotic adjustment. Glycine betaine and proline are both physiologic indicators of stresses interaction effect whereas trehalose indicates only water stress. The nitrate reductase activity can be used as water stress indicator and chlorophylls a, b and total are indicators to water or acid stress. The dry mass of shoot part, leaves number and leaf area are significantly reduced by stress in young sugarcane plants adjusted osmotically. The interaction of stresses cause decrease of dry mass while water stress reduces leaves number and leaf area.

Made available in DSpace on 2014-06-11T19:33:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-10-01Bitstream added on 2014-06-13T21:06:47Z : No. of bitstreams: 1 queiroz_rjb_dr_jabo.pdf: 3752046 bytes, checksum: eebdc1beb2a1be4824cb3db842e096f9 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Nesta pesquisa foi avaliado o metabolismo de dois genótipos de milho (Zea mays L.) ao estresse hídrico e a correlação entre o teor de compostos responsáveis pela a aclimatação à limitação hídrica desses genótipos na germinação e no estádio vegetativo. Foram instalados dois experimentos. Inicialmente, um ensaio de germinação foi montado com o objetivo de verificar a tolerância de dois genótipos de milho, DKB 390 e DAS 2B710 ao déficit hídrico e de quantificar a prolina e a trealose nos tecidos endospermático e embrionário com intuito de descrever as suas funções fisiológicas na germinação. O segundo avaliou o ajustamento osmótico através das respostas bioquímicas e fisiológicas e moleculares de dois híbridos de milho sob duas disponibilidades hídricas no solo, em um latossolo vermelho. A partir desses resultados, verificou-se a tolerância destes híbridos a seca nesse estádio e o reflexo da expressão relativa de genes relacionados à síntese de solutos compatíveis em reposta à limitação hídrica no solo / The metabolism of maize genotypes (Zea mays L.) to soil water avaibility and the correlation between the content of compounds responsible for this acclimation to water limitation of these genotypes at the germination and the silking stage. Two experiments were carried out. First, the germination test was evaluated the tolerance of two genotypes of maize, DKB 390 and DAS 2B710 to water available and also to quantify the proline and trehalose in the endosperm and embryonary axis tissues with the aim of describe their physiological functions in germination. The second assay it was examined the osmotic adjustment through biochemical and physiological responses of two hybrids growing in two soil water availability. The results of that experiment, checking the degree of tolerance of these hybrids and the reflection of the gene expression related to synthesis of these compatible solutes in response to soil water availability

Tesis (magíster con mención en derecho penal) / El siguiente trabajo tiene por objeto analizar la problemática de la penalización de las ganancias ilícitas en Chile, el origen de la discusión y el desarrollo de los argumentos a favor y contra de la existencia de un delito tributario por la no tributación de dichos ingresos. A su vez, se revisarán las opiniones más relevantes para la solución de este conflicto, mediante la sanción del enriquecimiento ilícito por vías distintas a la tipificación de un ilícito tributario, verificando que este no es un tema pacífico para la doctrina y jurisprudencia nacional y que se encuentra lejos de estar completamente resuelto.

Orientadora: Durvalina Maria Mathias dos Santos / Banca: Raffaella Rossetto / Banca: Marcelo de Almeida Silva / Banca: Marcos Omir Marques / Banca: David Ariovaldo Banzatto / Resumo: No presente trabalho foi verificado o ajuste osmótico no crescimento inicial de cana-de-açúcar, cv. IAC91-5155, sob efeito da interação dos estresses hídrico e ácido no solo. Para tanto, foram quantificados os teores de três solutos compatíveis, prolina, glicina betaína e trealose. Também foi estudada a atividade da nitrato redutase e os teores de clorofilas. O estudo de todas estas variáveis teve por objetivo propor a utilização destes compostos como indicadores fisiológicos de aclimatação da cana-deaçúcar sob efeito da interação de ambos os estresses. O experimento foi realizado em casa de vegetação com 29,7±4,3oC e 75±10 UR%. Foram utilizados três tratamentos de disponibilidade hídrica (capacidade de campo, CC): controle (70%), estresse moderado (55%) e estresse severo (40%) e três tratamentos de acidez no solo (saturação por bases, V%): controle (55%), média acidez (33%) e alta acidez (23%). O delineamento experimental utilizado foi o de blocos casualizados em esquema fatorial 3x3, com quatro repetições. Após 60 dias sob estresses foram determinados os teores dos solutos compatíveis, a atividade da nitrato redutase e os teores das clorofilas. Os resultados mostram que houve acúmulo dos três solutos compatíveis evidenciando que ocorreu ajuste osmótico. Prolina e glicina betaína são indicadores fisiológicos do efeito da interação de ambos os estresses enquanto trealose é indicadora apenas para o estresse hídrico. Também, a atividade da nitrato redutase pode ser utilizada como indicador do estresse hídrico e os teores das clorofilas a, b e total para os estresses hídrico ou ácido. A massa de matéria seca da parte aérea, o número de folhas e a área foliar das plantas jovens de cana-de-açúcar, ajustadas osmoticamente, são reduzidos pelos estresses. Os efeitos interativos de ambos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: In this present work was verified the osmotic adjustment in the initial plant growth sugarcane cv. IAC91-5155 under interaction effect of water and acid stress in the soil. For this, were quantified three compatible solutes content, as follows: proline, glycine betaine and trehalose. It was verified also the nitrate reductase activity as well the chlorophyll content. The study of all parameters aimed to propose the use components as physiological indicators to acclimation of sugarcane plants under interaction the both stress. The experiment was carried out in a greenhouse under 29.7±4.3oC and 75±10%. Three water availability treatments (Field Capacity - FC%): control (70%), moderate stress (55%), and extreme stress (40%) and three soil acidification treatment (Base Saturation - BS%): control (55%), average acidity (33%), and high acidity (23%) were used. The experimental design used was the randomized blocks in a 3x3 factorial scheme and four replicates. After sixty days under stress were determined compatible solutes, nitrate reductase and chlorophyll content. Our results showed that there was the accumulation of three compatible solutes exhibiting osmotic adjustment. Glycine betaine and proline are both physiologic indicators of stresses interaction effect whereas trehalose indicates only water stress. The nitrate reductase activity can be used as water stress indicator and chlorophylls a, b and total are indicators to water or acid stress. The dry mass of shoot part, leaves number and leaf area are significantly reduced by stress in young sugarcane plants adjusted osmotically. The interaction of stresses cause decrease of dry mass while water stress reduces leaves number and leaf area. / Doutor

An MRI’s superior soft tissue contrast and ability to perform parametric scanning make it a powerful tool for use during medical procedures; from surgery to rehabilitation. However, the MRI’s strong static magnetic field, fast switching gradients and constrained space make accomplishing procedures within it difficult. Recent advances in the field of robotics have enabled the creation of devices capable of assisting medical practitioners in this environment. In this work, technologies to enable the use and control of robotic assistive devices for MRI interventions are presented. This includes a modular controller that is designed, built and used to control two surgical systems with minimal effect on image quality. Progressive improvements to an MRI conditional actuator including the construction of a first of a kind plastic piezoelectric resonant motor stator that improves the motor’s compatibility with the MRI is presented. Finally, control algorithms are evaluated for real-time functional MRI based control of a rehabilitation robot which includes the use of a robot for controlling brain activity of a subject in an online experiment.

DNA-encoded peptide libraries are the basis for in vitro selection methods that use various biological systems (phage display; yeast display; mRNA display). Despite the great success of these selection methods, their obvious disadvantage is the limited number of building blocks, which consist of only twenty proteinogenic amino acids. The involvement of other non- proteinogenic amino acids and other building blocks could significantly expand the range of possible applications of these selection methods. For example, the introduction of chemical modifications in amino acid side chains in such libraries would allow the effective study of post-translational modifications (phosphorylation, acylation, glycosylation, methylation, etc.) in living organisms. The aim of this work was to develop a method for preparation of a fully synthetic DNA encoded library of peptides. The basic steps for the preparation were the chemical synthesis of the peptide and associated enzymatic synthesis of encoding DNA. Compatibility of chemical reactions with DNA is essential for the synthesis of DNA-encoded peptide libraries. Because the final acidic deprotection of the side chains in the peptide is not compatible with DNA, two approaches have been tested to overcome this problem. The first was an attempt to develop finer...

An MRI’s superior soft tissue contrast and ability to perform parametric scanning make it a powerful tool for use during medical procedures; from surgery to rehabilitation. However, the MRI’s strong static magnetic field, fast switching gradients and constrained space make accomplishing procedures within it difficult. Recent advances in the field of robotics have enabled the creation of devices capable of assisting medical practitioners in this environment. In this work, technologies to enable the use and control of robotic assistive devices for MRI interventions are presented. This includes a modular controller that is designed, built and used to control two surgical systems with minimal effect on image quality. Progressive improvements to an MRI conditional actuator including the construction of a first of a kind plastic piezoelectric resonant motor stator that improves the motor’s compatibility with the MRI is presented. Finally, control algorithms are evaluated for real-time functional MRI based control of a rehabilitation robot which includes the use of a robot for controlling brain activity of a subject in an online experiment.

Hierarchical arrangement of biological composites such as nacre and bone containing high filler (ceramic) content results in high strength and toughness of the natural material. In this study we mimic the design of layered bone microstructure and fabricate an optimal multifunctional bio-nanocomposite having strength, toughness and corrosion resistance. Poly (butylene adipate-co-terephthalate) (PBAT), a biodegradable polymer was used as a substrate material with the reinforcement of LDH (Layered double hydroxide) as a nanofiller in different concentrations to achieve enhancement in mechanical properties as well as processing related thermostability. Corrosion resistance was increased by mimicking a layered structured which incorporated a tortuous diffusion path.

Medium Mn TRIP steels are amongst the most widely researched third generation advanced high strength steels (3G-AHSSs) as they are ideal candidates for automotive light-weighting applications owing to their superior strength and ductility balance. However, the thermal processing cycles of these steels need to be compatible with the industrial continuous galvanizing line (CGL) in order to successfully employ them in the automotive manufacturing industry. The main objective of the present research was to develop a CGL compatible thermal processing cycle for a prototype medium Mn steel that would produce significant volume fractions of chemically stable retained austenite and exhibit mechanical properties consistent with established 3G-AHSS targets. In that regard, the effects of intercritical annealing (IA) time and temperature and starting microstructure were determined in the first part of this research. The as-received tempered martensite (S-TM) and heat treated martensite (S-M) were the two different starting microstructures studied in this research. In this case, the overaging temperature (OT) treatment (460°C for 20s) was kept constant. It was found that high volume fractions (≥ 0.30) of retained austenite were achieved for S-M samples intercritically annealed at 675°C for shorter times (i.e. 60 to 120s) compared to S-TM samples. TEM analysis of the S-M samples showed that most of the retained austenite was present in a film type morphology, which is known to be more stable chemically and mechanically compared to the block type morphology. The tensile test results showed that although both the S-TM and S-M samples exhibited a high strength/ductility balance, the S-M samples, particularly the S-M 675°C + 120s samples, showed more potential in terms of CGL compatibility and achieving 3G-AHSS target mechanical properties. The effect of OT holding time was determined in the second part of this research. In that regard, the OT holding time was varied form 20s to 120s for selected S-TM and S-M samples. The S-TM 710°C samples with increased OT holding times (60s and 120s) had a significant increase in retained austenite volume fraction compared to the baseline 20s OT samples. However, the retained austenite volume fractions did not change for S-M samples regardless of OT holding time. It was also found that the mechanical properties of the annealed S-TM and S-M steels depended on the OT holding time. For the S-TM samples with > 120s IA holding times, longer OT holding times (60s and 120s) produced chemically unstable retained austenite which transformed rapidly at low strain resulting in low UTS × TE products. However, although longer OT holding times significantly increased the yield strength of the annealed S-M samples, the UTS × TE product decreased significantly owing to decreased retained austenite stability. Finally, based on the results of this research, it was concluded that the prototype medium Mn TRIP steel can achieve 3G-AHSS target mechanical properties using CGL-compatible thermal processing cycles. Moreover, depending on successful reactive wetting, it may be possible to perform both thermal processing and galvanizing of this steel in the industrial CGL. / Thesis / Master of Applied Science (MASc)

Made available in DSpace on 2016-04-29T13:30:47Z (GMT). No. of bitstreams: 1 Geny Rodrigues Valadao.pdf: 795641 bytes, checksum: 9338d7ad0cbf144399f62c4b332485a0 (MD5) Previous issue date: 2005-09-12 / ABSTRACT The present work has as objective to contribute for a reflection process on the psychological aspects implied in the process of protection of the Programa de Apoio ás Testemunhas, Vítimas e Familiares de Vítimas de Violência - PROVITA, emphasizing the change of identity, compatible and incompatible personality, the role of the psychologist and the freedom in the clandestinity. It was based on articles published by GAJOP and material obtained with the representative of Secretaria de Promoção e Defensa dos Direitos Humanos, in Brasilia. The objective of the study was focalized in psychological aspects of victims and witness and about challenge that they face to develop a new personal structure based on values of their new environment. From the referencial of the critical theory, it became evident the contradictions existent in the process of protection of PROVITA to reduce physical and psychological discomfort of the victims and witness, the suffering and the traumatic breaking lived by the individual. The research brings a set of information on the public politics of security that a few years ago nobody thought about it in Brazil. It evidences some problems that PROVITA will still face to become a space of contradiction and desorganization of the artifice of the social, economic politician system that command and propitiate total poverty and absence of social and political projects that guarantee the executation of the Human Rights. / RESUMO O presente trabalho tem como objetivo contribuir para um processo de reflexão sobre os aspectos psicológicos implicados no processo de proteção do Programa de Apoio às Testemunhas, Vítimas e Familiares de Vítimas de Violência PROVITA, destacando a mudança de identidade, personalidade compatível e incompatível, o papel do psicólogo e a liberdade na clandestinidade. Foi fundamentado em artigos publicados pelo GAJOP e material obtido com o representante da Secretaria de Promoção e Defesa dos Direitos Humanos, em Brasília. O objeto de estudo centrou-se nos aspectos psicológicos das vítimas e testemunhas, bem como nos desafios que enfrentam para desenvolverem uma nova estrutura pessoal referenciada em valores de seu novo ambiente. A partir do referencial da teoria crítica, procurou-se evidenciar as contradições existentes no processo de proteção do PROVITA para diminuir o mal-estar físico e psicológico das vítimas e testemunhas, o sofrimento e a violação traumática. A pesquisa traz um conjunto de informações sobre a política pública de segurança que há anos era impensável no Brasil. Evidencia alguns problemas que o PROVITA ainda terá que enfrentar para se tornar um espaço de desmistificação e desmantelamento das artimanhas do sistema social, político e econômico que impera, domina e propicia a pobreza e a ausência de projetos sociais e políticos que garantam a efetivação dos Direitos Humanos.