Little is known about the enzyme-substrate interactions occurring during the dechlorination of chloroform (CF) and 1,1,1-trichloroethane (1,1,1-TCA) by the enrichment culture containing Dehalobacters, hereafter called DHB-CF/MEL. Compound specific isotope analysis (CSIA) is used to investigate the factors which may affect the isotope fractionation observed for CF and 1,1,1-TCA dechlorination. This thesis reports the first isotope enrichment factors observed for CF biodegradation at -27.5‰ ± 0.9‰, thus providing fundamental information for comparing isotope enrichment factors observed during trichlorinated alkane degradation by DHB-CF/MEL. The thesis also reports how the presence of CF and 1,1,1-TCA influences isotope fractionation and explores the possible influence of substrate inhibition on isotope fractionation during 1,1,1-TCA dechlorination. The data suggests that substrate inhibition during 1,1,1-TCA dechlorination by DHB-CF/MEL may not affect carbon isotope fractionation. The results suggest that CSIA is a promising monitoring tool even for the simultaneous biodegradation of CF and 1,1,1-TCA at different 1,1,1-TCA starting concentration.

<p>Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli however, exogenous factors can induce apoptosis. Many anti-cancer drugs work by activating apoptosis in cancer cells. Natural substances have been found to have the ability to induce apoptosis in various tumour cells and these substances have been used as templates for the construction of&nbsp / novel lead compounds in anticancer treatment. On the other hand, alkylating agents such as cisplatin, cis- [PtCl2 (NH3) 2] have been widely used as antineoplastic agents for a&nbsp / wide variety of cancers including testicular, ovarian, neck and head cancers, amongst others. However, the use of cisplatin as an anticancer agent is limited due to toxicity and resistance problems. The aim of this present study was to screen the leaves of Rhus laevigata, a South African indigenous plant, for the presence of pro-apoptotic and&nbsp / anti-proliferative natural compounds and also to screen newly synthesised palladium based complexes (15 and 57) and a platinum based complex (58) for their antineoplastic&nbsp / activities tested against a panel of cell lines. Results. The results showed that crude methanol extracts from Rhus laevigata as well as the newly synthesised palladium based complexes (15 and 57) and a platinum based complex (58) induced apoptosis in the cell lines tested, as demonstrated by the externalization of phosphatidylserine, mitochondrial membrane permeabilization,caspase-3 activation, and DNA fragmentation. Caski (cervical cancer) and H157 (non small cell lung carcinoma) cell lines treated with the methanol extract from Rhus laevigata however, were more resistant to apoptosis induction. Among the metallocomplexes, complexes 15 and 57, palladium based complexes, were the most active. Conclusion: The methanol extract from the leaves of Rhus laevigata contain pro-apoptotic and antiproliferative natural compound(s), which need to be characterised and elucidated as they could provide the much-needed lead compounds in the fight against cancer. On the other hand the newly synthesized palladium complexes also need further evaluation to&nbsp / see if they can be used as anticancer agents that can overcome the problems associated with cisplatin.</p>

Depleting oil reserves and increased costs of the oil and gas recoveries have created the need to drill in challenging formations. When drilled through, shale formations in particular always generated a wide variety of problems if conventional water-based muds are used. Furthermore, the complexity and variations in shales have compounded the task of developing suitable drilling fluids. In light of these problems, the study of shale properties and their interactions with fluids will continue to be a muchneeded source of information in drilling industry. In this study a low molecular weight quaternary amine compound, which is provided by KarKim Drilling Fluids Inc., and its mixtures with sodium and potassium chloride is investigated in the aspects of capillary suction times, hot rolling recoveries, methylene blue capacities and one-dimensional free swelling properties in order to compare performances of salts and amine compound. For all test methods quaternary amine concentration from 1% to 6% by volume were studied and seen that less amount of quaternary amine compound is needed in order to obtain close shale recoveries and shale volume change potential if compared with sodium and potassium chloride. This prevents the usage of high concentration of chlorides and hence provides environmental sensitiveness. Furthermore, it was concluded that combining an amount of salt with quaternary amine compound gives the system enhanced shale inhibition properties.

This study was concerned with the recovery of aroma compounds from aqueous solutions by pervaporation using poly(ether-block-amide) (PEBA) membranes. Three model aroma compounds (i.e., propyl propionate, C6-aldehyde and benzaldehyde) were used in the study to represent ester, aldehyde and aromatic aroma compounds, respectively. The effects of process conditions (i.e., feed concentration and operating temperature) on the pervaporation performance (in terms of permeation flux and selectivity) for aroma-water separations were investigated. It was found that both the aroma permeation flux and the selectivity were affected significantly by the feed aroma concentration. The aroma permeability was in the order of propyl propionate > C6-aldehyde > benzaldehyde, and the membrane selectivity for aroma/water separation followed the order of C6-aldehyde > propyl propionate > benzaldehyde. In general, the aroma flux was found to be proportional to the aroma compound concentration in the solution. In the concentration range (390-3,200 ppm) tested, the effect of temperature on the permeation flux followed an Arrhenius type of relation. The solubility and diffusivity of the aroma compounds in PEBA membrane, which determine their permeabilities through the membrane, were determined from the pervaporation and sorption/desorption data. It was shown that the solubility of the aroma compounds in the PEBA membrane generally followed the Henry’s law where the sorption uptake was proportional to the feed aroma concentration. Among the three aroma compounds studied, benzaldehyde was found to have the highest solubility selectivity in the PEBA membrane, followed by C6-aldehyde and propyl propionate. The solubilities of pure propyl propionate and water in PEBA membrane were also estimated; the solubility of pure propyl propionate was around 130 times higher than that of pure water. This confirmed that PEBA was an excellent organophilic membrane. The diffusivity of the aroma compounds through PEBA membrane was affected by the feed aroma concentration. From steady state pervaporation and equilibrium sorption data, the diffusivity was calculated on the basis of solution-diffusion model, and the diffusivity was shown to be linearly dependent on the feed aroma concentration. On the other hand, from the sorption kinetics data obtained from the time-dependent sorption experiments, the diffusivity was shown to be affected by the feed aroma concentration exponentially. The main reason may be that the simple form of the solution-diffusion model is unable to precisely describe the mass transport through the membrane during pervaporation. As an alternative to pervaporation where the liquid feed is in contact with the membrane and the mass transport involves permeation and evaporation (thus the word “pervaporation”), evaporation-permeation (or evapermeation, where the feed liquid is not in direct contact with the membrane and the mass transfer involves evaporation and then permeation) was also studied for aroma compound separation from water. It was shown that evapermeation was no better than pervaporation in terms of permeation flux and selectivity. This again demonstrated that the state of the membrane and the location for liquid-vapor phase change were important to the mass transport through the membrane. For aroma recovery from dilute aqueous solutions, batch pervaporation is often preferred. Batch pervaporation coupled with permeate decantation and water phase recycle was studied parametrically. It was demonstrated that compared to the conventional pervaporation, the aroma recovery can be enhanced by recycling the water phase from the permeate decanter to the feed for further recovery. In addition, unlike the conventional batch operation where the product concentration starts to decrease beyond certain time, the modified batch pervaporation allows a longer period of operation without compromising the product purity.

The English translation of the Swedish compound fönsterbräda into windowsill, or the proper noun Danmark into Denmark makes perfect sense. But how about the compound fossilbränslefri as simply fossil fuel or the name Mälaren as Lake?  All four of these translations have been produced with the help of automatic machine translation. The aim of this paper is to present the expanding field of application of machine translation and some issues related to this type of translation. More specifically, the study has looked at Google Translate as one of the most commonly used machine translation systems online, and how it responds to the two linguistic categories that were selected for this small study: compound nouns and proper nouns. Besides analyzing these categories, two different text types were chosen: general information articles from a local authority website (Stockholm City) and patent texts, both of which belong to the expanding field of application of Google Translate. The results of the study show that in terms of compound nouns, neither of the text types proved to be significantly better suited for machine translation than the other and neither had an error rate below 20 %. Most of the errors related to words being erroneously omitted in the English output and words which were incorrectly translated in relation to context. As for proper nouns, the patent texts contained none and subsequently no error analysis could be made, whereas the general information articles included 76 proper nouns (out of a total word count of 810). The most prominent error related to the Swedish version not being maintained in the English output where it should have been, e.g. translating Abrahamsberg as Abraham rock. The errors in both of the linguistic categories had varying impact on the meaning of the texts, some of which distorted the meaning of the word completely, and some which were of minor importance. This factor, along with the fact that the reader of the text influences how the comprehension level of the text is perceived through their language and subject knowledge, makes it difficult to evaluate the full impact of the various errors. It can, however, be said that patent text could pose as a better option for machine translation than general information articles in relation to proper nouns, as this text type is likely to contain no or very few proper nouns.

Over the last years, the emission and on board diagnostics legislations for heavy duty trucks are getting more and more strict. An accurate engine model that is possible to execute in the engine control system enables both better diagnosis and lowered emissions by better control strategies. The objective of this thesis is to extend an existing mean value diesel engine model, to include turbo compound. The model should be physical, accurate, modular and it should be possible to execute in real time. The calibration procedure should be systematic, with some degree of automatization. Four different turbo compound models have been evaluated and two models were selected for further evaluation by integration with the existing model. The extended model showed to be quite insensitive to small errors in the compound turbine speed and hence, the small difference in accuracy of the tested models did not affect the other output signals significantly. The extended models had better accuracy and could be executed with longer step length than the existing model, despite that more complexity were added to the model. For example, the mean error of the intake manifold pressure at mixed driving was approximately 3.0%, compared to 5.8% for the existing model. The reasons for the improvements are probably the good performance of the added submodels and the systematic and partly automatized calibration procedure including optimization.

This study was concerned with the recovery of aroma compounds from aqueous solutions by pervaporation using poly(ether-block-amide) (PEBA) membranes. Three model aroma compounds (i.e., propyl propionate, C6-aldehyde and benzaldehyde) were used in the study to represent ester, aldehyde and aromatic aroma compounds, respectively. The effects of process conditions (i.e., feed concentration and operating temperature) on the pervaporation performance (in terms of permeation flux and selectivity) for aroma-water separations were investigated. It was found that both the aroma permeation flux and the selectivity were affected significantly by the feed aroma concentration. The aroma permeability was in the order of propyl propionate > C6-aldehyde > benzaldehyde, and the membrane selectivity for aroma/water separation followed the order of C6-aldehyde > propyl propionate > benzaldehyde. In general, the aroma flux was found to be proportional to the aroma compound concentration in the solution. In the concentration range (390-3,200 ppm) tested, the effect of temperature on the permeation flux followed an Arrhenius type of relation. The solubility and diffusivity of the aroma compounds in PEBA membrane, which determine their permeabilities through the membrane, were determined from the pervaporation and sorption/desorption data. It was shown that the solubility of the aroma compounds in the PEBA membrane generally followed the Henry’s law where the sorption uptake was proportional to the feed aroma concentration. Among the three aroma compounds studied, benzaldehyde was found to have the highest solubility selectivity in the PEBA membrane, followed by C6-aldehyde and propyl propionate. The solubilities of pure propyl propionate and water in PEBA membrane were also estimated; the solubility of pure propyl propionate was around 130 times higher than that of pure water. This confirmed that PEBA was an excellent organophilic membrane. The diffusivity of the aroma compounds through PEBA membrane was affected by the feed aroma concentration. From steady state pervaporation and equilibrium sorption data, the diffusivity was calculated on the basis of solution-diffusion model, and the diffusivity was shown to be linearly dependent on the feed aroma concentration. On the other hand, from the sorption kinetics data obtained from the time-dependent sorption experiments, the diffusivity was shown to be affected by the feed aroma concentration exponentially. The main reason may be that the simple form of the solution-diffusion model is unable to precisely describe the mass transport through the membrane during pervaporation. As an alternative to pervaporation where the liquid feed is in contact with the membrane and the mass transport involves permeation and evaporation (thus the word “pervaporation”), evaporation-permeation (or evapermeation, where the feed liquid is not in direct contact with the membrane and the mass transfer involves evaporation and then permeation) was also studied for aroma compound separation from water. It was shown that evapermeation was no better than pervaporation in terms of permeation flux and selectivity. This again demonstrated that the state of the membrane and the location for liquid-vapor phase change were important to the mass transport through the membrane. For aroma recovery from dilute aqueous solutions, batch pervaporation is often preferred. Batch pervaporation coupled with permeate decantation and water phase recycle was studied parametrically. It was demonstrated that compared to the conventional pervaporation, the aroma recovery can be enhanced by recycling the water phase from the permeate decanter to the feed for further recovery. In addition, unlike the conventional batch operation where the product concentration starts to decrease beyond certain time, the modified batch pervaporation allows a longer period of operation without compromising the product purity.

Dhana ya usarufishaji, kama Kamusi ya Isimu na Lugha inavyoeleza, ni ubadilishaji wa neno huru ama mofimu huru yenye maana ya kisemantiki na kuifanya mofimu funge na yenye maana ya kisarufi zaidi. Tunaposema kuhusu usarufishaji, mara nyingi tunamaanisha hasa ubadilishaji wa neno huru liwe mofimu. Miongoni mwa aina zote za maneno, ambazo zinaweza kusarufishwa, zile zinazotumika mara nyingi zaidi ni nomino na vitenzi. Ningetaka kujibu swali juu ya kazi ya kisarufi ya leksimu mwana: baada ya kuzichunguza maana zake za kisarufi inawezekana kutilia mkazo kwamba katika lugha ya Kiswahili tunashuhudia mwanzo wa kuibadilisha nomino huru mwana iwe kiambishi awali cha uundaji wa maneno mapya yanayotaja watu mbalimbali kutokana na kazi, shughuli zao, kuwepo katika vyama n.k. Inawezekana kwamba maneno ambatani yanayoanzia na mwana yanaunda ngeli maalum ya nomino (sawa na ngeli ya 1a/2a ya lugha nyingine za Kibantu).

In this study, a chlorinated-solvent contaminated groundwater site was used as the study site. Multivariate statistical analysis explains the huge and complicated current situation of the original data efficiently, concisely, and explicitly; it simplifies the original data into representative factors, or bases on the similarity between data to cluster and identify clustering outcome. The statistical software SPSS 12.0 was used to perform the multivariate statistical analysis to evaluate groundwater quality characteristics of this site. Results show that 20 analytical items of groundwater quality of the study site are simplified into seven major representative factors through factor analysis, including ¡§background¡¨, ¡§salt residual¡¨, ¡§hardness¡¨, ¡§ethylene chloride¡¨, ¡§alkalinity¡¨, ¡§organic pollutant¡¨, and ¡§chloroform¡¨. The factor score diagram was drawn according to the score of monitoring well on each factor and 89.6% of the variance could be obtained. This study used cluster analysis to cluster in two phrases, the groundwater quality monitoring wells were classified into seven clusters according to the similarity of monitored data nature and the differences between clusters. The groundwater quality characteristics and pollutant distributions of each cluster out this site were evaluated. The clustering result indicates that for the sixth cluster (where monitoring well SW-6 was the representative well), the average concentrations of chlorides such as 1,1-dichloroethylene, 1,1-dichloroethane, and cis-1,2-dichloroethylene were the highest among the clusters, indicating those the groundwater of nearby area might be polluted by chlorinated organic compounds. In addition, to evaluate whether the clustering of cluster analysis were appropriate or not, discriminant analysis is used to evaluate clustering accuracy, in which seven Fisher discriminant coefficient formulas that were exclusively suitable for this location were established. Then, the observed values were substituted to Fisher discriminant coefficient formula. Result shows that the monitoring well¡¦s clusters obtained from discriminant analysis were totally identical with the result of actual cluster analysis; the accuracy were 100%. After performing cross-validation analysis, the result shows that the accuracy were 80%, indicating the use of discriminant analysis (with forecasting function) to verify the clustering result of the cluster analysis was highly accurate. After analyzing the pollution condition of this site using time trend and space distribution, it were determined to conclude that trichloroethylene and 1,1-dichloroethylene were the major concerning pollutants; the pollutants appeared to be spreading on a large scale, so it was difficult to use the existing data to evaluate the pollution source. After assessing environmental medium characteristics and pollutant distribution of the site, this study suggests that the use of insitu bioremediation, which is cost-effective, can be applied as a remedial mothod.

In past years, legislative pressures (particularly in Japan and Europe) had forced the electronics industry to eliminate Pb from their end products and manufacturing processes. With respect to factors such as ease of converting existing tin-lead plating systems, ease of manufacture and compatibility with existing assembly methods, pure tin plating is seen by many in the industry as a potentially simple and cost effective alternative to SnPb-based systems. The problem of spontaneous tin whisker formation, a characteristic of pure tin, still needs to be addressed, as it can lead to device failure by shorting two terminals on electronic devices. This possibility gives rise to major reliability concerns. The study relates to an electronic component with pure tin deposit layer on the part for electric connection, wherein pure tin deposit layer is a fine grained tin deposit layer composed of grains with smaller size in the direction perpendicular to the deposit surface than in the direction parallel to the deposit surface. It is called irregular tin grain structure. It applies a process for plating an electronic component, so as to form a pure tin deposit layer on the part for electric connection, comprising the steps of: adjusting the composition of tin plating solution in which starter additive and brighter additive are included; moving the electronic component through the tin plating solution, so as to form a fine grained tin deposit layer on the part for electric connection. We performed a DoE by depositing different tin grain structures with variant thickness. After whisker test in high temperature/high humidity and room condition, we confirmed corrosion mechanism, intermetallic morphology, and different behaviour of tin atoms. To summarize the studies, as compared with the prior arts, irregular grain structure can validly inhibit the whisker growth.