Osteoarthritis (OA) is a chronic joint disease characterized by irreversible cartilage degradation. MMP (matrix metalloproteinase) inhibitors represent a new approach to slowing OA progression by addressing cartilage degradation mechanisms. However, the success of preclinical studies failed to be translated into clinical application. One of the possible reasons is that the disease models in preclinical study can't reflect the biological complexity of human disease. Hydrogel-based cartilage constructs as in vitro models have shown promise as preclinical testing platforms due to their enhanced physiological relevance, improved prediction to human response, high-throughput drug screening, and ease of use. Metalloproteinase-13 (MMP-13) is thought to be a major contributor to the degradation of articular cartilage in OA by aggressively breaking down type II collagen. This study focused on testing MMP-13 inhibitors using a GelMA-alginate hydrogel-based OA model induced by cytokines interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α). The results demonstrated a significant inhibition of type II collagen breakdown by measuring C2C concentration using ELISA after treatment with MMP-13 inhibitors. Therefore, the study highlights the GelMA-alginate hydrogel-based OA model as an alternative to human-sourced cartilage explants for in vitro drug screening, which can improve the predictability and relevance of preclinical evaluations of MMP-13 inhibitors for osteoarthritis, thereby complementing existing 2D culture, cartilage explant, and animal model studies and addressing the translational gap observed in clinical trials.

There is an apparent lack of in-depth knowledge about the processes that may underlie the interaction between work and home life, and their relationships with employee health and well-being. Work and home has traditionally been considered as separate domains, but during the past decade of democracy, transformation developments (eg, Affirmative Action, Employment Equity) changed the nature of the labour market and economy. This facilitated the increase in the number of working single-parent, dual-earner families, and of women participating in the workforce, which in turned influenced the work-home interaction of employed individuals. Furthermore, various researchers regard burnout and engagement as important constructs to consider in the well-being of employees. The level of a person's wellbeing subsequently affects his/her functionality in both the work and home spheres. Thus, there is a need to identify ways which both the individual and the organisation can apply to increase personal well-being and the balance between work and home life. South Africa has 11 different national languages, and only 8,3% of the population actually speak English at home. Language differences should therefore be taken into account when administering questionnaires. Studies in South Africa generally report race, education, language, and understanding of English as the main factors which impact on construct and item comparability of psychometric tests. There is consequently an obvious need to translate research instruments before they are administered to individuals from different language groups. If language differences are not taken into account, invalid conclusions regarding the constructs under study could be made, with serious implications for culturally diverse settings such as in South Africa. The objectives of this research were to translate the Survey Work-Home Interaction Nijmegen (SWING), Maslach Burnout Inventory-General Survey (MBI-GS) and Utrecth Work Engagement Scale (UWES) into Afrikaans and Setswana, and to investigate the construct validity, construct equivalence and reliability of these instruments. Furthermore, differences between demographic groups regarding work-home interaction and well-being were investigated. Finally, a structural model was tested, which included job characteristics, negative and positive work-home interference (WHI) and well-being (burnout and engagement). A cross-sectional survey design was used. Random samples (N = 685) were taken from police stations in the North West province. Structural equation modelling (SEM) was used to determine the construct validity and equivalence of the measuring instruments and to test the structural model. The results indicated that work-home interaction can be described as a four-dimensional construct consisting of negative WHI, positive WHI, negative home-work interference (HWI), and positive HWI. This factor structure was equivalent across all three language groups and all the scales were reliable. A four-factor model was confirmed for burnout and included exhaustion, cognitive weariness, cynicism and professional efficacy. A one-factor model was found for engagement. Both translated instruments were found to be equivalent for the three language groups. Furthermore, a second order factor analysis revealed that the underlying structure of well-being consists of two negatively related and equivalent factors, namely burnout (exhaustion, cognitive weariness and cynicism) and an enlarged engagement construct (engagement and professional efficacy). Members reported more negative WHI than negative HWI, and more positive HWI than positive WHI. Statistically significant differences exist between demographic groups regarding work-home interaction based on language, gender, marital status. parental status and education. Statistically significant differences of wellness exist between demographic groups based on language and educational level. The results of the structural equation modelling revealed that job demands were directly and positively associated with negative WHI and burnout, while job resources were directly and positively associated with positive WHI and work engagement. This also indicates the partial mediating effect of WHI between job characteristics and wellness. In addition, a lack ofjob resources was associated with higher levels of burnout. Recommendations for future research were made. / Thesis (Ph.D. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2007.

Major changes that emphasise the importance of a balanced work and family life have occurred within organisations. Inability to manage this integration between the work and home domains can affect the performance of organisations and the wellbeing of their employees. In order to measure work-home interaction and wellbeing, it is important to use valid, equivalent and reliable instruments. However, there is a lack of empirical research when investigating work-home interaction, burnout and work engagement in the earthmoving equipment industry in South Africa. Furthermore, the earthmoving equipment industry is a multicultural environment and construct equivalence across subgroups therefore becomes important when measuring work-home interaction, burnout and work engagement across different cultural or language groups. It also seems relevant to consider differences in the work-home interaction and work wellness levels between important demographic groups in the earthmoving equipment industry. It appears as if burnout and engagement can be seen as part of the total wellness continuum; and that job characteristics may be related to burnout and engagement through negative and positive work-home interaction. However, little information was found that included these factors in a structural model for the earthmoving equipment industry. The objectives of this research were to test the psychometric properties of the Survey Work- Home Interaction - Nijmegen (SWING), the Maslach Burnout Inventory - General Survey (MBI-GS) and the Utrecht Work Engagement Scale (UWES); to determine if various demographic groups differ with regard to work-home interaction and wellbeing; and to test a structural model that includes job characteristics, work-home interaction and wellbeing for employees in the earthmoving industry. A cross-sectional survey design was used. Random samples (n = 528) were taken from employees in the earthmoving equipment industry in Gauteng, the Limpopo Province, Mpumalanga, the Northern Cape, the Western Cape, the Eastern Cape, Kwa-Zulu Natal and the North West Province. Structural equation modelling confirmed a four-factor model of work-home interaction which measures negative work-home interference, positive work-home interference, negative homework interference and positive home-work interference. This factor structure was equivalent across language, ethnicity, gender, education, marital status and parental status. All four factors were reliable. The participants reported more negative WHI than negative HWI, and more positive HWI than positive WHI. It was found that statistically significant differences that are based on age, gender, language, ethnicity, a partner's contribution to household income and the different business units exist between the demographic groups. The results confirmed a four-factor structure of burnout (exhaustion, cognitive weariness, cynicism and professional efficacy) and a two-factor structure of work engagement (including vigour and dedication). These structures were equivalent across language and education groups, and all scales were reliable. SEM analyses supported a two-factor structure for the wellness construct, consisting of burnout (exhaustion, cognitive weariness and cynicism) and engagement (vigour, dedication and professional efficacy), which was equivalent for the language and education groups. Statistically significant differences that are based on age, gender, language and the different business units were found between the demographic groups. Structural equation modelling showed that job demands are associated with negative WHI and consequently with burnout, providing support for a full-mediation effect of negative WHI. Job resources were associated with negative WHI and consequently with burnout, providing support for partial mediation of negative WHI between job resources and burnout. Job resources were also associated with positive WHI and consequently with engagement, providing support for partial mediation of positive WHI between job resources and engagement. Recommendations for the organisation and future research were made. / Thesis (Ph.D. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2007.

O objetivo principal deste trabalho é refletir sobre como é construída a relação entre definitude e Caso no hebraico por meio da análise do Construct State e do fenômeno da Marcação Diferencial de Objeto. Dessa forma, pretendemos com essa pesquisa suscitar reflexões sobre o fenômeno da Definitude Espraiada, sobre a Teoria do Caso, sobre a formação do Construct State e dos compounds na sintaxe e sobre a semântica do et. Utilizamos para a análise dos dados o arcabouço teórico da Morfologia Distribuída (Cf. HALLE; MARANTZ (1993), HALLE (1997) e MARANTZ (1997)), além das últimas observações feitas acerca do Programa Minimalista (Cf. CHOMSKY (1998, 2001)), ambos desenvolvimentos recentes da Teoria Gerativa. Assim sendo, de modo diferente de outras análises do Construct State, que não levam em consideração os compostos, nossa análise proporciona uma explicação para a formação, tanto do Construct State, quanto dos compounds, na sintaxe, focalizando a estrutura de cada uma dessas construções: o primeiro possuindo uma estrutura composta de duas raízes abstratas e o segundo constituído apenas por uma raiz. A estrutura dos compounds procura refletir a não composicionalidade entre os dois nomes que o formam. Já a estrutura do Construct State privilegia a composicionalidade dos membros do construto, a Definitude Espraiada e a não modificação direta do núcleo. Enfim, sugerimos que a relação entre Caso e definitude no hebraico seja uma relação formal e dependente. Formal, porque ela se expressa por meio dos traços dos nominais que devem ser valorados, checados, mantidos para a inserção de conteúdo fonológico e, até mesmo, inseridos tardiamente pelo Componente Morfológico. Dependente, porque sem a definitude, Caso não pode ser checado. / The main aim of this work is to investigate the relationship between definiteness and Case in Hebrew. This will be done by analyzing the Construct State and the phenomenon of Differential Object Marking. By doing so, we intend to shed some light on the phenomenon of Definiteness Spreading, on the Case Theory, on the Construct State and syntactic compounds and on the semantics of ´et. In order to analyze the data, we worked within the framework of Distributed Morphology (Cf. HALLE; MARANTZ (1993), HALLE (1997) e MARANTZ (1997)), considering, as well, the Minimalist Program (Cf. CHOMSKY (1998, 2001)), both of them, recent developments of Generative Theory. Due to this fact - and differently from other Construct State analysis that dont take the compounds into consideration our analysis provides an explanation for the formation of both the Construct State and the syntactic compounds. This is achieved by focusing on the structure of these constructions: the former is a structure derived from two abstract roots, and the later is formed by a single root. The structure of the compounds aims at reflecting the non-compositional interpretation, which is typical in these cases. The Construct State structure, on the other hand, emphasizes the compositionality in its interpretation, the Definiteness Spreading and the non-direct modification of the head. In other words, we suggest that the relationship between Case and definiteness in Hebrew is a formal and dependent one. It is formal because it is expressed by the features of the nominal that must be valued, checked, maintained for proper insertion of phonological content, and late-inserted by the Morphological Component; and it is dependent because Case cannot be checked without definiteness.

O objetivo principal deste trabalho é refletir sobre como é construída a relação entre definitude e Caso no hebraico por meio da análise do Construct State e do fenômeno da Marcação Diferencial de Objeto. Dessa forma, pretendemos com essa pesquisa suscitar reflexões sobre o fenômeno da Definitude Espraiada, sobre a Teoria do Caso, sobre a formação do Construct State e dos compounds na sintaxe e sobre a semântica do et. Utilizamos para a análise dos dados o arcabouço teórico da Morfologia Distribuída (Cf. HALLE; MARANTZ (1993), HALLE (1997) e MARANTZ (1997)), além das últimas observações feitas acerca do Programa Minimalista (Cf. CHOMSKY (1998, 2001)), ambos desenvolvimentos recentes da Teoria Gerativa. Assim sendo, de modo diferente de outras análises do Construct State, que não levam em consideração os compostos, nossa análise proporciona uma explicação para a formação, tanto do Construct State, quanto dos compounds, na sintaxe, focalizando a estrutura de cada uma dessas construções: o primeiro possuindo uma estrutura composta de duas raízes abstratas e o segundo constituído apenas por uma raiz. A estrutura dos compounds procura refletir a não composicionalidade entre os dois nomes que o formam. Já a estrutura do Construct State privilegia a composicionalidade dos membros do construto, a Definitude Espraiada e a não modificação direta do núcleo. Enfim, sugerimos que a relação entre Caso e definitude no hebraico seja uma relação formal e dependente. Formal, porque ela se expressa por meio dos traços dos nominais que devem ser valorados, checados, mantidos para a inserção de conteúdo fonológico e, até mesmo, inseridos tardiamente pelo Componente Morfológico. Dependente, porque sem a definitude, Caso não pode ser checado. / The main aim of this work is to investigate the relationship between definiteness and Case in Hebrew. This will be done by analyzing the Construct State and the phenomenon of Differential Object Marking. By doing so, we intend to shed some light on the phenomenon of Definiteness Spreading, on the Case Theory, on the Construct State and syntactic compounds and on the semantics of ´et. In order to analyze the data, we worked within the framework of Distributed Morphology (Cf. HALLE; MARANTZ (1993), HALLE (1997) e MARANTZ (1997)), considering, as well, the Minimalist Program (Cf. CHOMSKY (1998, 2001)), both of them, recent developments of Generative Theory. Due to this fact - and differently from other Construct State analysis that dont take the compounds into consideration our analysis provides an explanation for the formation of both the Construct State and the syntactic compounds. This is achieved by focusing on the structure of these constructions: the former is a structure derived from two abstract roots, and the later is formed by a single root. The structure of the compounds aims at reflecting the non-compositional interpretation, which is typical in these cases. The Construct State structure, on the other hand, emphasizes the compositionality in its interpretation, the Definiteness Spreading and the non-direct modification of the head. In other words, we suggest that the relationship between Case and definiteness in Hebrew is a formal and dependent one. It is formal because it is expressed by the features of the nominal that must be valued, checked, maintained for proper insertion of phonological content, and late-inserted by the Morphological Component; and it is dependent because Case cannot be checked without definiteness.

The Human Papillomavirus (HPV) is a common sexually transmitted infection (STI) and etiological agent of cervical cancer. It has been suggested that religiosity may promote a generalized disassociation between all STIs/STDs, including HPV, and personal relevance thus contributing to lower levels of HPV awareness and knowledge among certain groups. This study sought to identify the role of religiosity as a predictor of HPV awareness and knowledge among women in a Christian university. Religiosity was defined and measured using the Duke University Religion Index (DUREL). The schemata and social identity theories provided the theoretical framework for this study. A total of 173 women completed the modified survey instrument to assess level of HPV awareness and knowledge, attitudes toward premarital sex, and level of religiosity. Multiple linear regression analysis was used to test the hypotheses. Religiosity, as defined by the intrinsic religiosity subscale of the DUREL significantly contributed to HPV awareness (p = .002) and HPV knowledge (p = .036). The positive social change implications of this study include a better understanding of the role of religiosity in HPV awareness and knowledge; consideration of religiosity in dissemination of health information by informing public health policies and programs to ensure adequate and culturally relevant education and awareness about HPV transmission, HPV-related cancers, and HPV vaccination.

[Truncated abstract] At the commencement of this research project in February 2005, there was a paucity of empirical investigations that focused on understanding the psychological construct of mental toughness in sport (Bull, Shambrook, James, & Brooks, 2005; Fourie & Potgieter, 2001; Jones, Hanton, & Connaughton, 2002). Although impressive, the available literature did little in offering consensus in terms of a definition and operationalising the construct in a consistent manner as well as understanding those factors contributing to its measurement and development. The absence of theoretically guided research, in particular, was noted as a major limitation of this research. The potential significance of mental toughness for performance excellence combined with the conceptual confusion and lack of rigorous empirical research highlighted the need for further research on mental toughness in sport. Accordingly, the purpose of this thesis was to examine issues pertaining to the understanding, measurement, and development of mental toughness in sport, using personal construct psychology (Kelly, 1955/1991) as the guiding theoretical framework. Australian football was chosen as the context to explore these issues. In the opening empirical chapter, two qualitative manuscripts in which Australian football coaches’ perspectives on mental toughness and those factors contributing to its development are reported. Three central themes for understanding mental toughness in Australian football were generated: characteristics (self-belief, motivation, tough attitude, concentration and focus, resilience, handling pressure, personal values, emotional intelligence, sport intelligence, and physical toughness); situations (e.g., injuries, success); and behaviours (e.g., superior decision-makers, consistent performance). '...' In the final empirical chapter, two manuscripts in which the effectiveness of two different psychological skills training programs in enhancing mental toughness among youth-aged (15's) Australian footballers are reported. The first presents a quantitative analysis while the second presents a qualitative analysis. Multisource ratings (self, parent, and coach) of the AfMTI and self-reported resilience and flow indicated more positive changes in mental toughness, resilience, and flow than the control group. Similar patterns in the findings were evident across rating sources. Interviews with several players and one of their parents as well as the coaches generated their perceptions on the benefits of participating in the program (e.g., increased work ethic, tougher attitudes) and the processes by which the program had an effect (e.g., self-awareness; self-monitoring; self-regulation; and multi-perspective discussions) as well as suggestions for program improvement (e.g., parent and coach education programs). In summary, the results of the five studies presented in this thesis provide a comprehensive account of issues pertaining to the understanding, measurement, and development of mental toughness in Australian football. The findings are supportive of several aspects of previous research but also extend this line of inquiry in a number of ways. It is my hope that other researchers will be stimulated to engage in further research extending what is presented here and that practitioners will use this information to inform their professional endeavours.

There is an apparent lack of in-depth knowledge about the processes that may underlie the interaction between work and home life, and their relationships with employee health and well-being. Work and home has traditionally been considered as separate domains, but during the past decade of democracy, transformation developments (eg, Affirmative Action, Employment Equity) changed the nature of the labour market and economy. This facilitated the increase in the number of working single-parent, dual-earner families, and of women participating in the workforce, which in turned influenced the work-home interaction of employed individuals. Furthermore, various researchers regard burnout and engagement as important constructs to consider in the well-being of employees. The level of a person's wellbeing subsequently affects his/her functionality in both the work and home spheres. Thus, there is a need to identify ways which both the individual and the organisation can apply to increase personal well-being and the balance between work and home life. South Africa has 11 different national languages, and only 8,3% of the population actually speak English at home. Language differences should therefore be taken into account when administering questionnaires. Studies in South Africa generally report race, education, language, and understanding of English as the main factors which impact on construct and item comparability of psychometric tests. There is consequently an obvious need to translate research instruments before they are administered to individuals from different language groups. If language differences are not taken into account, invalid conclusions regarding the constructs under study could be made, with serious implications for culturally diverse settings such as in South Africa. The objectives of this research were to translate the Survey Work-Home Interaction Nijmegen (SWING), Maslach Burnout Inventory-General Survey (MBI-GS) and Utrecth Work Engagement Scale (UWES) into Afrikaans and Setswana, and to investigate the construct validity, construct equivalence and reliability of these instruments. Furthermore, differences between demographic groups regarding work-home interaction and well-being were investigated. Finally, a structural model was tested, which included job characteristics, negative and positive work-home interference (WHI) and well-being (burnout and engagement). A cross-sectional survey design was used. Random samples (N = 685) were taken from police stations in the North West province. Structural equation modelling (SEM) was used to determine the construct validity and equivalence of the measuring instruments and to test the structural model. The results indicated that work-home interaction can be described as a four-dimensional construct consisting of negative WHI, positive WHI, negative home-work interference (HWI), and positive HWI. This factor structure was equivalent across all three language groups and all the scales were reliable. A four-factor model was confirmed for burnout and included exhaustion, cognitive weariness, cynicism and professional efficacy. A one-factor model was found for engagement. Both translated instruments were found to be equivalent for the three language groups. Furthermore, a second order factor analysis revealed that the underlying structure of well-being consists of two negatively related and equivalent factors, namely burnout (exhaustion, cognitive weariness and cynicism) and an enlarged engagement construct (engagement and professional efficacy). Members reported more negative WHI than negative HWI, and more positive HWI than positive WHI. Statistically significant differences exist between demographic groups regarding work-home interaction based on language, gender, marital status. parental status and education. Statistically significant differences of wellness exist between demographic groups based on language and educational level. The results of the structural equation modelling revealed that job demands were directly and positively associated with negative WHI and burnout, while job resources were directly and positively associated with positive WHI and work engagement. This also indicates the partial mediating effect of WHI between job characteristics and wellness. In addition, a lack ofjob resources was associated with higher levels of burnout. Recommendations for future research were made. / Thesis (Ph.D. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2007.

Major changes that emphasise the importance of a balanced work and family life have occurred within organisations. Inability to manage this integration between the work and home domains can affect the performance of organisations and the wellbeing of their employees. In order to measure work-home interaction and wellbeing, it is important to use valid, equivalent and reliable instruments. However, there is a lack of empirical research when investigating work-home interaction, burnout and work engagement in the earthmoving equipment industry in South Africa. Furthermore, the earthmoving equipment industry is a multicultural environment and construct equivalence across subgroups therefore becomes important when measuring work-home interaction, burnout and work engagement across different cultural or language groups. It also seems relevant to consider differences in the work-home interaction and work wellness levels between important demographic groups in the earthmoving equipment industry. It appears as if burnout and engagement can be seen as part of the total wellness continuum; and that job characteristics may be related to burnout and engagement through negative and positive work-home interaction. However, little information was found that included these factors in a structural model for the earthmoving equipment industry. The objectives of this research were to test the psychometric properties of the Survey Work- Home Interaction - Nijmegen (SWING), the Maslach Burnout Inventory - General Survey (MBI-GS) and the Utrecht Work Engagement Scale (UWES); to determine if various demographic groups differ with regard to work-home interaction and wellbeing; and to test a structural model that includes job characteristics, work-home interaction and wellbeing for employees in the earthmoving industry. A cross-sectional survey design was used. Random samples (n = 528) were taken from employees in the earthmoving equipment industry in Gauteng, the Limpopo Province, Mpumalanga, the Northern Cape, the Western Cape, the Eastern Cape, Kwa-Zulu Natal and the North West Province. Structural equation modelling confirmed a four-factor model of work-home interaction which measures negative work-home interference, positive work-home interference, negative homework interference and positive home-work interference. This factor structure was equivalent across language, ethnicity, gender, education, marital status and parental status. All four factors were reliable. The participants reported more negative WHI than negative HWI, and more positive HWI than positive WHI. It was found that statistically significant differences that are based on age, gender, language, ethnicity, a partner's contribution to household income and the different business units exist between the demographic groups. The results confirmed a four-factor structure of burnout (exhaustion, cognitive weariness, cynicism and professional efficacy) and a two-factor structure of work engagement (including vigour and dedication). These structures were equivalent across language and education groups, and all scales were reliable. SEM analyses supported a two-factor structure for the wellness construct, consisting of burnout (exhaustion, cognitive weariness and cynicism) and engagement (vigour, dedication and professional efficacy), which was equivalent for the language and education groups. Statistically significant differences that are based on age, gender, language and the different business units were found between the demographic groups. Structural equation modelling showed that job demands are associated with negative WHI and consequently with burnout, providing support for a full-mediation effect of negative WHI. Job resources were associated with negative WHI and consequently with burnout, providing support for partial mediation of negative WHI between job resources and burnout. Job resources were also associated with positive WHI and consequently with engagement, providing support for partial mediation of positive WHI between job resources and engagement. Recommendations for the organisation and future research were made. / Thesis (Ph.D. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2007.

Thesis (Ph. D.)--Miami University, Dept. of Psychology, 2007. / Title from second page of PDF document. Includes bibliographical references (p. 156-158).