The Chromosomal Passenger Complex (CPC) is a major regulator of mitosis composed of the catalytic subunit Aurora B, the inner centromere protein INCENP, Survivin and Borealin/Dasra B. The CPC controls aspects of mitosis, ranging from chromosome and spindle structure to the correction of kinetochore-microtubule attachment errors, regulation of mitotic progression and completion of cytokinesis (Carmena et al., 2012). Knocking down any one CPC component induces delocalization of the others and disrupts mitotic progression (Adams et al., 2001 ; Carvalho et al., 2003; Lens et al., 2003 ; Gassmann et al., 2004; Vader et al., 2006). Telophase Disc (TD-60), also known as RCC2, is a putative Guanine Exchange Factor (GEF) that has been suggested to be involved in completion of cytokinesis through GTPase activation (Mollinari et al., 2003). However, its mechanism of action is still unclear. Interestingly, TD-60 has a typical Chromosomal Passenger Complex (CPC) localization (Andreassen et al., 1991) and its down-regulation alters CPC localisation during early mitosis. However, it is not a member of the CPC immunoprecipitated from mitotic cells (Gassmann et al., 2004). Here, I improved human TD-60 recombinant protein production by expressing a synthetic cDNA in the baculovirus expression system. This allowed me to characterize TD-60-associated GEF activity in vitro and study its possible influence on core CPC activity in vivo. I tested purified human TD-60 against a broad selection of GTPase targets, representing each GTPase family, in an established GEF assay. My data demonstrated that TD-60 has consistent high GEF activity in vitro towards the Ras-like protein A, RalA. To understand if TD-60 links RalA GTPase function to the CPC in vivo, I performed TD-60 and RalA RNAi experiments in HeLa cells. Interestingly, both TD-60 and RalA-depleted cells exhibit destabilized kinetochore fibers, a similar defective prometaphase-like bipolar spindle structure, and an abnormal centromeric accumulation of the CPC in early mitosis. In order to confirm that phenotypes seen after TD-60 depletion were due to lack of RalA activation in vivo, I generated a constitutively active RalA mutant that I transfected into TD-60- deficient cells. Strikingly, the RalA Q72L active mutant (mimicking the GTP-bound form) rescued the abnormal bipolar spindle structure, corrected the defective kinetochore-microtubules attachments, and rescued the atypical CPC distributions observed at centromeres after TD-60 depletion. These results suggest that TD-60-associated RalA GEF activity stabilizes kinetochore-microtubule attachments in early mitosis and that, TD-60 links RalA GTPase function to the CPC during mitosis.

The chromosomal passenger complex (CPC: Aurora B-INCENP-Survivin-Borealin) is a key regulator of cell division whose localisation at centromeres is required for stable kinetochore-microtubule attachments and proper chromosomal segregation (Ruchaud et al. 2007; Carmena et al. 2012; van der Waal et al. 2012). Shugoshin1 (hSgo1) (via Borealin) and Histone H3 (via Survivin) have been implicated in centromeric targeting of CPC (Wang et al. 2010; Jeyaprakash et al. 2011; Tsukahara et al. 2010; Kawashima et al. 2010). Although the Survivin-Histone H3 pathway has been extensively studied, the intermolecular interactions dictating CPC-hSgo1 interactions remain unclear. My PhD work focused on characterising the molecular framework of the CPC-hSgo1 interaction using biochemical, biophysical and structural biology methods. I optimised and improved human CPC and hSgo1 recombinant protein production in an E. coli system. Post optimisation, I used Size-Exclusion Chromatography to successfully reconstitute the CPC-hSgo1 complex in vitro and further confirmed that hSgo1 possessing no modification or extra amino acids on its N-terminus can interact with Survivin and Borealin-Survivin-INCENP1-57. This suggested that the hSgo1 N-terminal tail interaction with Survivin is crucial for CPC-hSgo1 interaction. Furthermore, I conducted calorimetric binding studies to molecularly dissect the individual contributions of CPC components and their domains towards CPC-hSgo1 interaction. Towards this aim, I expressed and purified different versions of CPC and analysed their binding energetics with hSgo1. The results from these experiments clearly suggested the contribution of Borealin and INCENP towards CPC-hSgo1 interaction.

O trabalho busca interpretar as reflexões teóricas de Oduvaldo Vianna Filho sobre teatro e engajamento entre 1958 e 1974 à luz do desenrolar da história do país e dos volteios no desenvolvimento da cultura naquele período. Vianinha, além de participar ativamente de momentos-chave da história do teatro brasileiro, como o Teatro de Arena, o CPC da UNE e o Grupo Opinião, tem uma significativa produção reflexiva sobre este desenvolvimento do teatro dito participante. Seu trabalho especulativo é um dos mais expressivos documentos da história do teatro naqueles anos. Por meio da análise de dois artigos exemplares do pensamento do autor, a saber, Do Arena ao CPC e Um pouco de pessedismo não faz mal a ninguém, escritos em períodos absolutamente distintos, 1962 e 1968, respectivamente, separados pela bruta ruptura de 1964, o trabalho tenta observar a oscilação característica das ideias de Vianna como parte do desenvolvimento truncado e conservador do Brasil. / The study aims to interpret the theoretical reflections of Oduvaldo Vianna Filho about theater and engagement between 1958 and 1974 in the light of the progress of the country\'s history and the spins in the development of culture in that period. Vianinha, besides actively participating in key moments in the history of Brazilian theater, such as Teatro de Arena (Arena Theatre), the CPC (Popular Centre of Culture) of UNE (National Students Union) and Grupo Opinião (Group Opinion), also has a significant reflexive production on the development of the so called participant theater. His speculative work is one of the most significant documents in the history of theater in those years. Through the analysis of two exemplary articles of the author\'s thought, namely, Do Arena ao CPC (From Arena to CPC) and Um pouco de pessedismo não faz mal a ninguém (A little pessedismo does not hurt anyone), written in two completely different periods, 1962 and 1968, respectively, separated by the gross disruption of 1964, this work attempts to observe the oscillation characteristic of Vianna\'s ideas as part of the truncated and conservative development of Brazil.

Submitted by Aline Martins (1146629@mackenzie.br) on 2016-09-13T00:19:00Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) PATRICIA SATOMI NISHIMURA.pdf: 1352150 bytes, checksum: 276ec9409624dbcc612995b34cf49235 (MD5) / Approved for entry into archive by Paola Damato (repositorio@mackenzie.br) on 2016-09-20T14:55:59Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) PATRICIA SATOMI NISHIMURA.pdf: 1352150 bytes, checksum: 276ec9409624dbcc612995b34cf49235 (MD5) / Made available in DSpace on 2016-09-20T14:55:59Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) PATRICIA SATOMI NISHIMURA.pdf: 1352150 bytes, checksum: 276ec9409624dbcc612995b34cf49235 (MD5) Previous issue date: 2016-08-04 / In Brazil, according to CPC 12- Present value adjustment (2008), the use of information based on the present value allows correction judgments about already registered past events and it improves the way of present events recognition. This means that, at the initial time of operation, the adjustment to present value should be estimated, considering cash flows of the corresponding operation (value, date and all terms and contract conditions). In this context, the present study sought to map the process of implementing CPC 12 at Telhanorte, by examining the difficulties and benefits of this procedure. The effects of CPC 12 in the Balance Sheet (BS) and Statement Net Income (SNI) were simulated. The expectations of users and accounting information makers were tried to be described in relation to this implementation and possible changes in the behavior of the company vendors were found, arising from this new accounting procedure, since the change in the sales revenue measurement could affect the salespeople’s behavior at the selling time. This work is an interventional study, since the researcher involved in the company's daily and focuses on the application of theory in the organizational environment. To collect the information needed to process mapping questionnaires and interviews were used, and the processing of data was used content analysis. It was concluded that the application of CPC 12 in Telhanorte has impacts on the financial statements, and the company must analyze the favorable and unfavorable factors in this CPC implementation process to decide whether or not to apply this accounting pronouncement, since the impacts they found showed changes in management, tax and accounting aspects. / No Brasil, de acordo com o pronunciamento técnico CPC 12 - Ajuste a valor presente (2008), a utilização de informações com base no valor presente permite a correção de julgamentos acerca de eventos passados já registrados e melhora a forma de reconhecimento de eventos presentes. Isso significa que no momento inicial da operação deve ser calculado o ajuste a valor presente, considerando os fluxos de caixa da correspondente operação (valor, data e todos os termos e as condições contratados). Nesse contexto, este estudo buscou mapear o processo de implementação do CPC 12 na Telhanorte, examinando as dificuldades e os benefícios desse procedimento. Foram simulados os efeitos do CPC 12 no Balanço Patrimonial (BP) e na Demonstração do Resultado do Exercício (DRE). Procurou-se descrever as expectativas dos usuários e preparadores da informação contábil em relação a essa implementação e verificar se as possíveis mudanças no comportamento dos vendedores da empresa decorrente desse novo procedimento contábil, já que a mudança na mensuração das receitas de vendas poderia mudar o comportamento dos vendedores na hora de vender. Este trabalho é uma pesquisa intervencionista, já que o pesquisador intervém no cotidiano da empresa e tem foco na aplicação da teoria no ambiente organizacional. Para coletar as informações necessárias ao mapeamento dos processos foram utilizados questionários e entrevistas, e no tratamento dos dados foi utilizada análise de conteúdo. Concluiu-se que a aplicação do CPC 12 na Telhanorte tem impactos nas Demonstrações Contábeis, sendo que a empresa deve analisar os fatores favoráveis e desfavoráveis no processo de implementação desse CPC para decidir se deve ou não aplicar esse pronunciamento contábil, uma vez que os impactos encontrados demonstraram alterações nos aspectos gerenciais, fiscais e contábeis.

Le but de cette étude est d’exploiter les fonctionnalités des nano-Composites Polymères Conducteurs (CPC) imprimés en utilisant la technologie FDM (modélisation par dépôt de monofilament en fusion) pour le développement de textiles fonctionnels et intelligents. L’impression 3D présente un fort potentiel pour la création d’une nouvelle classe de nanocomposites multifonctionnels. Par conséquent, le développement et la caractérisation des polymères et nanocomposites fonctionnels et imprimables en 3D sont nécessaires afin d’utiliser l’impression 3D comme nouveau procédé de dépôt de ces matériaux sur textiles. Cette technique introduira des procédés de fonctionnalisation de textiles plus flexibles, économes en ressources et très rentables, par rapport aux procédés d'impression conventionnels tels que la sérigraphie et le jet d'encre. L’objectif est de développer une méthode de production intégrée et sur mesure pour des textiles intelligents et fonctionnels, afin d’éviter toute utilisation d'eau, d'énergie et de produits chimiques inutiles et de minimiser les déchets dans le but d’améliorer l'empreinte écologique et la productivité. La contribution apportée par cette thèse consiste en la création et la caractérisation de filaments CPC imprimables en 3D, le dépôt de polymères et de nanocomposites sur des tissus et l’étude des performances en termes de fonctionnalité des couches de CPC imprimées en 3D. Dans un premier temps, nous avons créé des filaments de CPC imprimables en 3D, notamment des nanotubes de carbone à parois multiples (MWNT) et du noir de carbone à haute structure (Ketjenblack) (KB), incorporés dans de l'acide polylactique (PLA) à l'aide d'un procédé de mélange à l'état fondu. Les propriétés morphologiques, électriques, thermiques et mécaniques des filaments et des couches imprimées en 3D ont été étudiées. Deuxièmement, nous avons déposé les polymères et les nanocomposites sur des tissus à l’aide d’une impression 3D et étudié leur adhérence aux tissus. Enfin, les performances des couches de CPC imprimées en 3D ont été analysées sous tension et force de compression appliquées. La variation de la valeur de la résistance correspondant à la charge appliquée permet d’évaluer l'efficacité des couches imprimées en tant que capteur de pression / force. Les résultats ont montré que les nanocomposites à base de PLA, y compris MWNT et KB, sont imprimables en 3D. Les modifications des propriétés morphologiques, électriques, thermiques et mécaniques des nanocomposites avant et après l’impression 3D nous permettent de mieux comprendre l’optimisation du procédé. De plus, différentes variables du procédé d’impression 3D ont un effet significatif sur la force d'adhérence des polymères et des nanocomposites déposés sur les tissus. Nous avons également développé des modèles statistiques fiables associés à ces résultats valables uniquement pour le polymère et le tissu de l’étude. Enfin, les résultats démontrent que les mélanges PLA/MWNT et PLA/KB sont de bonnes matières premières piézorésistives pour l’impression 3D. Elles peuvent être potentiellement utilisées dans l’électronique portable, la robotique molle et la fabrication de prothèses, où une conception complexe, multidirectionnelle et personnalisable est nécessaire. / The aim of this study is to get the benefit of functionalities of fused deposition modeling (FDM) 3D printed conductive polymer nanocomposites (CPC) for the development of functional and smart textiles. 3D printing holds strong potential for the formation of a new class of multifunctional nanocomposites. Therefore, development and characterization of 3D printable functional polymers and nanocomposites are needed to apply 3D printing as a novel process for the deposition of functional materials on fabrics. This method will introduce more flexible, resource-efficient and cost-effective textile functionalization processes than conventional printing process like screen and inkjet printing. The goal is to develop an integrated or tailored production process for smart and functional textiles which avoid unnecessary use of water, energy, chemicals and minimize the waste to improve ecological footprint and productivity. The contribution of this thesis is the creation and characterization of 3D printable CPC filaments, deposition of polymers and nanocomposites on fabrics, and investigation of the performance of the 3D printed CPC layers in terms of functionality. Firstly, the 3D printable CPC filaments were created including multi-walled carbon nanotubes (MWNT) and high-structured carbon black (Ketjenblack) (KB) incorporated into a biobased polymer, polylactic acid (PLA), using a melt mixing process. The morphological, electrical, thermal and mechanical properties of the 3D printer filaments and 3D printed layers were investigated. Secondly, the performance of the 3D printed CPC layers was analyzed under applied tension and compression force. The response for the corresponding resistance change versus applied load was characterized to investigate the performance of the printed layers in terms of functionality. Lastly, the polymers and nanocomposites were deposited on fabrics using 3D printing and the adhesion of the deposited layers onto the fabrics were investigated. The results showed that PLA-based nanocomposites including MWNT and KB are 3D printable. The changes in morphological, electrical, thermal, and mechanical properties of nanocomposites before and after 3D printing give us a great understanding of the process optimization. Moreover, the results demonstrate PLA/MWNT and PLA/KB as a good piezoresistive feedstock for 3D printing with potential applications in wearable electronics, soft robotics, and prosthetics, where complex design, multi-directionality, and customizability are demanded. Finally, different variables of the 3D printing process showed a significant effect on adhesion force of deposited polymers and nanocomposites onto fabrics which has been presented by the best-fitted model for the specific polymer and fabric.

No description available.

No description available.

O International Financial Reporting Interpretation Comittee 12 (IFRIC 12) foi o primeiro pronunciamento internacional sobre contratos de concessões. Pela complexidade do tema, demorou vários anos para ser publicado e aplicado, mas algumas discussões internacionais ainda persistem. No Brasil, o IFRIC 12 foi traduzido na Interpretação do Comitê de Pronunciamentos Contábeis 01 (ICPC 01). O objetivo deste trabalho foi estudar as diferentes formas de contratação das concessões rodoviárias e suas contabilizações. Para isso, foi feito um estudo de caso em uma holding de concessões brasileira, análise de conteúdo dos relatórios anuais de outras concessionárias concorrentes e dos contratos de concessões, além de entrevistas sobre outorgas onerosas na Agência Reguladora de Serviços Públicos Delegados de Transporte do Estado de São Paulo (ARTESP) e Associação Brasileira de Concessionárias de Rodoviárias (ABCR). As três modalidades principais de concessões rodoviárias: gratuita, onerosa e subsidiada geram diferenças significativas na estrutura patrimonial da empresa, afetando, de forma distinta, as contas de ativo intangível, ativo financeiro, outorgas fixas e variáveis, provisões de manutenção, além de receitas, podendo resultar em impactos variados no patrimônio líquido. O resultado indicou que ainda há oportunidades para melhorias na representação contábil dos direitos e obrigações das concessionárias. / The International Financial reporting Interpretation Committee 12 (IFRIC 12) was the first international pronouncement about concessions arrangements. The subject was discussed for many years until be published and applied, due to the complexity of the issue. However, the debate is not finished yet. In Brazil, the IFRIC 12 was translated on Interpretação do Comitê de Pronunciamentos Contábeis 01 (ICPC 01). The aim of this study was to study the different hiring forms of the road concessions and its accountings. So, a case study in a concession holding was made, compared with others concessionaires, besides interview about onerous grants at Agência Reguladora de Serviços Públicos Delegados de Transporte do Estado de São Paulo (ARTESP) and at Associação Brasileira de Concessionarias de Rodoviárias (ABCR). The three main forms of road concessions: free, onerous and subsidized can produce differences on the financial structure of the company, affecting differently, the intangible assets, financial assets, fixed and variable grants, provision of maintenance, and revenues accounts, impacting the equity. The result showed that the financial statement can be better.

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro / Esta dissertação apresenta a avaliação térmica, econômica e ambiental de um sistema de aquecimento solar (SHS) que é usado em uma usina de asfalto, através de simulação computacional com TRNSYS. O processo escolhido é o aquecimento do betume a partir da temperatura de armazenamento até a temperatura de mistura, usando óleo mineral como fluido de transferência de calor (HTF). Os componentes do sistema são o trocador de calor HTF-betume, o coletor concentrador solar parabólico composto (CPC), o aquecedor auxiliar e a bomba de circulação. A simulação no TRNSYS calcula os balanços de massa e energia no circuito fechado do HTF a cada hora. Dados horários do Ano Meteorológico Típico (TMY) do Rio de Janeiro foram utilizados para executar este trabalho. Em muitos casos, a temperatura do HTF ultrapassou 238C, mostrando que o CPC é apropriado para esta aplicação. Economia de combustível e emissões evitadas foram consideradas para as análises economica e ambiental. Este trabalho descreve as fontes renováveis de energia, os tipos de usinas de asfalto e de aquecedores de betume. Ele também mostra a fração brasileira de algumas destas fontes. Os resultados, portanto, mostram ser possível encorajar políticas públicas ambientalmente corretas para incentivar o uso de energia solar na indústria de asfalto. Além disso, este trabalho pode ajudar na redução da elevada emissão dos gases de efeito estufa a partir da utilização dos combustíveis fósseis nesta indústria. / This dissertation presents thermal, economic and environmental evaluation of a solar heating system (SHS) which is used in an asphalt plant from computational simulation with TRNSYS. The process chosen is the bitumen heating from the storage up to the mixing temperature, using mineral oil as heat transfer fluid (HTF). The system components are the HTF-bitumen heat exchanger, the compound parabolic concentration solar collector (CPC), the auxiliary heater and the circulation pump. The TRNSYS simulation computes the mass and energy balances in the HTF closed loop every hour. Rio de Janeiro typical meteorological year (TMY) hourly weather data was used in order to perform this paper. In many instances, HTF temperature has reached a temperature that more than 238C, showing that the CPC is suitable for this application. Fuel savings and avoided emissions were taken into account for economic and environmental analysis. In this work describes the renewable energy sources, the asphalt plant and bitumen heater types. It also shows the Brazilian portion of the some of these sources. The results, though, made it possible to address environmentally sound public policies to encourage solar energy use in the Asphalt Industry. Moreover, it will help in reducing the high emission of the green house gases from the use of the fossil fuels in this industry.

O International Financial Reporting Interpretation Comittee 12 (IFRIC 12) foi o primeiro pronunciamento internacional sobre contratos de concessões. Pela complexidade do tema, demorou vários anos para ser publicado e aplicado, mas algumas discussões internacionais ainda persistem. No Brasil, o IFRIC 12 foi traduzido na Interpretação do Comitê de Pronunciamentos Contábeis 01 (ICPC 01). O objetivo deste trabalho foi estudar as diferentes formas de contratação das concessões rodoviárias e suas contabilizações. Para isso, foi feito um estudo de caso em uma holding de concessões brasileira, análise de conteúdo dos relatórios anuais de outras concessionárias concorrentes e dos contratos de concessões, além de entrevistas sobre outorgas onerosas na Agência Reguladora de Serviços Públicos Delegados de Transporte do Estado de São Paulo (ARTESP) e Associação Brasileira de Concessionárias de Rodoviárias (ABCR). As três modalidades principais de concessões rodoviárias: gratuita, onerosa e subsidiada geram diferenças significativas na estrutura patrimonial da empresa, afetando, de forma distinta, as contas de ativo intangível, ativo financeiro, outorgas fixas e variáveis, provisões de manutenção, além de receitas, podendo resultar em impactos variados no patrimônio líquido. O resultado indicou que ainda há oportunidades para melhorias na representação contábil dos direitos e obrigações das concessionárias. / The International Financial reporting Interpretation Committee 12 (IFRIC 12) was the first international pronouncement about concessions arrangements. The subject was discussed for many years until be published and applied, due to the complexity of the issue. However, the debate is not finished yet. In Brazil, the IFRIC 12 was translated on Interpretação do Comitê de Pronunciamentos Contábeis 01 (ICPC 01). The aim of this study was to study the different hiring forms of the road concessions and its accountings. So, a case study in a concession holding was made, compared with others concessionaires, besides interview about onerous grants at Agência Reguladora de Serviços Públicos Delegados de Transporte do Estado de São Paulo (ARTESP) and at Associação Brasileira de Concessionarias de Rodoviárias (ABCR). The three main forms of road concessions: free, onerous and subsidized can produce differences on the financial structure of the company, affecting differently, the intangible assets, financial assets, fixed and variable grants, provision of maintenance, and revenues accounts, impacting the equity. The result showed that the financial statement can be better.