Tyrimo tikslas – išvystyti analitinę metodiką tinkamą geležies sulfato, folio rūgšties ir cianokobalamino įvertinimui jiems esant daugiakomponenčio vaistinio preparato sudėtyje. Uždaviniai: surinkti informaciją apie anemiją ir medicinoje vartojamus antianeminius preparatus; apžvelgti plačiausiai naudojamus folio rūgšties, cianokobalamino ir geležies tyrimo metodus; pasirinkta metodika atlikti daugiakomponenčio preparato Ferro-folgamma komponentų atskyrimą; pasirinktais metodais identifikuoti cianokobalaminą, folio rūgštį, geležies sulfate; atlikti folio rūgšties ir geležies kiekybinę analizę. Metodai: efektyvioji skysčių chromatografija, spektrofotometrija. Tyrimo objektas – vaistinis preparatas “Ferro-folgamma”, kurio vienoje kapsulėje yra 100 mg bevandenio dvivalentės geležies sulfato (atitinka 37 mg geležies), 5 mg folio rūgšties, 10 μg cianokobolamino. Tyrimo rezultatai. ESC su diodų matricos detektoriumi metodika atliktas folio rūgšties ir cianokobalaminio atskyrimas ir nustatymas. Vitaminų atskyrimas pasiektas naudojant 150×4.6 mm 3 µm ACE18 kolonėlę, judriąją fazę fluoracto rūgštį (0.1 proc.) ir acetonitrile, judrios fazės tekmės greičiui esant 1.0 ml/min. Folio rūgšties detekcija atlikta prie 287 nm ilgio bangos, nustatyta aptikimo riba – 0.04 µg/ml, kiekybinio nustatymo riba – 0.13 µg/ml. Cianokobalamino detekcija atlikta prie 360 nm bangos ilgio, nustatyta aptikimo riba – 0.474 µg/ml, kiekybinio nustatymo riba – 1.57 µg/ml. Spektrofotometriškai kiekybiškai nustatyta... [toliau žr. visą tekstą] / Aim – to develop an analytical method that would be suitable for evaluating iron sulphate, folic acid and cyanocobalamin in the multicomponental drug. Objectives: gather information about anemia and antianemic drugs used in medicine; review the most often used methods of folic acid, cyanocobalamin and iron; carry out the separation of “Ferro-folgamma” components; identify cyanocobalamin, folic acid and iron sulphate; carry out a quantitive determination of folic acid and iron. Methods: high-performance liquid chromatography, spectrophotometry. Object: preparation “Ferro-folgamma” containing 100 mg anhydrous iron sulphate (37 mg of iron), 5 mg folic acid and 10 μg cyanocobalamin. Results: Identification and determination of folic acid and cyanocobalamin in pharmaceutical preparation was developed using HPLC with photodiode array detector (PDA). Folic acid and cyanocobalamin were separated on a ACE18 column (150×4.6 mm, 3 µm particle size) with a gradient elution of mobile phase consisting of 0.1% trifluoroacetic acid and acetonitrile. The separation was achieved with a flow rate of 1.0 ml/min. Detection of folic acid was performed at 287 nm wavelength, limit of detection – 0.04 µg/ml, limit of quantification – 0.13 µg/ml. Detection of cyanocobalamin was performed at 360 nm wavelength, limit of detection – 0.474 µg/ml, limit of quantification – 1.57 µg/ml. The absorbance of the iron complex was measured spectrophotometrically at 423 nm. Conclusions: HPLC/PDA method for... [to full text]

Hexachloro-1,3-butadiene (HCBD) is a toxic aliphatic chlorinated hydrocarbon which is widely used as a fungicide, herbicide and heat transformer fluid. HCBD is resistant to microbial degradation and, therefore, persists in aquatic and soil environments worldwide. In this thesis, the ability of non-specific bacteria from various sources to dechlorinate HCBD in the presence of either acetate or lactate (as an electron donor) and cyanocobalamin (as an electron shuttle) under different conditions was investigated. Cultivating specific populations to reduce cyanocobalamin as a method to increase HCBD dechlorination rate was investigated. Also, the factors responsible for HCBD dechlorination and the stalling of dechlorination were studied. Lastly, redox potential measurement during the microbial reductive dechlorination of HCBD for online detection of ongoing dechlorination was evaluated. Findings from the Project „« Non-specific bacteria from activated sludge, anaerobic digested effluent from municipal waste, piggery waste and sheep rumen content are able to dechlorinate HCBD in the presence of cyanocobalamin to chlorine-free C4 gases in a biochemical reaction. „« Dechlorination was equated to the formation of completely dechlorinated end-products from HCBD dechlorination. „« Methanogens were found to be involved in HCBD dechlorination. „« Mediators rather than specific bacteria were responsible for the fast dechlorination rates. Results suggest that activated sludge may release synthesized mediators into the supernatant to enable enhanced HCBD dechlorination. „« HCBD dechlorination can be monitored using oxidation reduction potential (ORP). ORP has an effect on HCBD dechlorination rate. Scientific Significance/Novelty The most significant finding from this research is that it demonstrates chlorine-free end-products in contrast with other studies in literature (Booker and Pavlosthasis, 2000; Bosma et al., 1994) where dechlorination was equated with disappearance of HCBD into bacterial biomass and the detection of partially dechlorinated gases such as trichlorobutadiene. It also shows that, in contrast to literature where specific bacteria (i.e., pure strains/cultures) were commonly used for the dechlorination of polychlorinated hydrocarbons, results from this thesis show that non-specific bacteria were able to dechlorinate HCBD in the presence of cyanocobalamin at ratesƒx sufficiently high to be considered for bioremediation projects. Moreover, results demonstrate that ORP can be used to monitor HCBD dechlorination.

Made available in DSpace on 2014-08-20T13:32:54Z (GMT). No. of bitstreams: 1 dissertacao_rubens_alves_pereira.pdf: 581091 bytes, checksum: 129866c31d5af8b07ef3e33c3e342067 (MD5) Previous issue date: 2010-02-26 / The aim of this study was to determine the effects of intramuscular injection of Butaphosphan and Cyanocobalamin on the parameters of energy metabolism, protein, enzyme, mineral and livestock of dairy cows in the postpartum period. We used 52 animals of the Holstein breed. The experimental period ranged from birth to 150 days of lactation. These cows were divided into 3 groups: BC10 (n=18), who received 5 doses of 10mL of solution of Butaphosphan+Cyanocobalamin, BC20 (n=18), who received 5 doses of 20mL of Butaphosphan+Cyanocobalamin; and CG (n=16), used as a control group received 5 doses of 10mL of saline (NaCl 0.9%). Administrations were performed with an interval of 5 days, intramuscularly, and blood samples every 5 days. Evaluations were performed on body condition score (BCS) and productive performance during the postpartum period and measurements of metabolic parameters such as blood glucose, nonesterified fatty acid (NEFA), urea, calcium (Ca), phosphorus (P), magnesium (Mg), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT) and ketone (CC) in urine. The measurements of blood glucose were not changed during the study period. The blood levels of NEFA were higher (P<0.05) for the GC compared to BC10 and BC20, which also differed (P<0.05). Urea levels did not differ between groups (P>0.05). There were no differences (P>0.05) in the metabolites Ca, P, Ca: P, Mg and CC in the urine. The same finding was common to liver enzymes, AST and GGT. The animals in groups BC10 and BC20 had a milk production higher than the GC (P<0.05), but did not differ (P>0.05). The analysis of milk components were similar (P>0.05) between groups. These results demonstrated that the use of Butaphosphan and Cyanocobalamin in dairy cows during the postpartum period, is effective in alleviating the negative energy balance and improving the productive performance, without causing liver overload. / O objetivo deste estudo foi determinar os efeitos da administração de Butafosfan e Cianocobalamina sobre os parâmetros do metabolismo energético, protéico, enzimático, mineral, produção e composição do leite de vacas leiteiras durante o período pós-parto. Foram utilizados no experimento 52 vacas da raça Holandês, avaliadas do parto até 150 dias de lactação. Estas vacas foram divididas em 3 grupos experimentais: o BC10 (n=18), que recebeu 5 doses de 10mL de solução aquosa de Butafosfan+Cianocobalamina; o BC20 (n=18), que recebeu 5 doses de 20mL de solução aquosa de Butafosfan+Cianocobalamina; e o grupo controle (GC n=16), que recebeu 5 doses de 10mL de solução fisiológica (NaCl 0,9%). Foram realizadas administrações logo após o parto, com intervalo de 5 dias, por via intramuscular. Foi coletado sangue a cada 15 dias a partir do terceiro dia pós-parto para a determinação dos níveis de glicose, ácidos graxos não esterificados (NEFA), β-hidroxibutirato (BHBA), uréia, cálcio (Ca), fósforo (P), magnésio (Mg), aspartato aminotransferase (AST) e gama-glutamiltransferase (GGT). Foi coletada urina a cada 15 dias a partir do terceiro dia pós-parto para a determinação de corpos cetônicos (CC). Avaliou-se o escore de condição corporal (ECC) e produção leiteira. Todos os grupos diferiram entre si quanto aos níveis de NEFA, sendo maiores no GC. Os grupos BC10 e BC20 diferiram entre si, sendo superiores no BC10. Os níveis de BHBA foram maiores no GC em relação ao BC10 e BC20, os quais não diferiram entre si. Quanto às avaliações zootécnicas, os grupos BC10 e BC20 apresentaram uma produção leiteira maior que o GC, porém não diferiram entre si. As concentrações dos componentes do leite não foram alteradas até 150 dias da lactação. Estes resultados demonstraram que a solução de Butafosfan e Cianocobalamina, administradas em vacas leiteiras após o parto, reduz o balanço energético negativo e aumenta a produção de leite, sem sobrecarregas o fígado.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / A ExtraÃÃo do terceiro molar à um procedimento comum, freqÃentemente associado com dor de intensidade de moderada ou grave, e que afeta um nÃmero significante de pacientes, o que torna os estudos clÃnicos que avaliam a dor associada com a exodontia dos terceiros molares relativamente fÃceis de se executar. O objetivo deste estudo foi determinar a eficÃcia e a seguranÃa da combinaÃÃo terapÃutica de piroxicam, dexametasona, citrato de orfenadrina e cianocobalamina (RheumazinÂ) em comparaÃÃo ao piroxicam isoladamente (FeldeneÂ) em um modelo de cirurgia do terceiro molar inferior. Neste estudo, que foi do tipo randomizado e duplo cego, foram incluÃdos oitenta pacientes selecionados para a exodontia do terceiro molar inferior. Os pacientes receberam Rheumazin ou Feldene 30 min depois da extraÃÃo do terceiro molar inferior e, a partir daÃ, uma vez ao dia durante 4 dias sucessivos. A intensidade da dor foi determinada por meio de uma escala visual analÃgica e pela quantidade de medicaÃÃo de escape utilizada pelos pacientes (paracetamol). O edema facial foi avaliado atravÃs de medidas da face usando uma escala mÃtrica calibrada (mm). Ainda, foram tambÃm avaliados os efeitos adversos e o grau de satisfaÃÃo em relaÃÃo aos tratamentos, ambos relatados pelos pacientes. Embora os resultados tenham mostrado que nÃo houve diferenÃa estatÃstica no edema facial entre os dois grupos, Rheumazin reduziu a dor na 6a e 120a h de tratamento. Rheumazin tambÃm demonstrou um melhor perfil de seguranÃa, prevenindo efeitos adversos tais como nÃusea, indisposiÃÃo e dor epigÃstrica, dentre outros, quando comparado ao FeldeneÂ. Este achado torna o Rheumazin uma boa opÃÃo para o tratamento no pÃs-operatÃrio da remoÃÃo do terceiro molar inferior. / Third molar extraction is a common procedure with pain frequently moderate or severe in intensity, and with sufficient numbers of patients to make studies relatively easy to perform. The aim of this study was to determine the efficacy and the safety of the therapeutic combination of piroxicam, dexamethasone, orphenadrine citrate and cyanocobalamin (RheumazinÂ) in comparison to piroxicam alone (FeldeneÂ) in a mandibular third molar surgery model. Eighty patients selected for removal of the third molar were included in this study which was randomized and double blind. They received Rheumazin or Feldene po 30 min after tooth extraction and once daily for 4 consecutive days. Pain was determined by a visual analogue scale and by the need for escape analgesia (paracetamol). Facial swelling was evaluated using a tape measuring method. Record of adverse effects and patientâs satisfaction was also considered. Although the results showed that there was no statistically significant difference in facial swelling between the two groups, Rheumazin reduced pain at 6th and 120th h of treatment. Rheumazin also demonstrated a better safety profile preventing adverse effects including nausea, indisposition and epigastric pain, among others, when compared to FeldeneÂ. This fact makes Rheumazin a good choice for post-surgery treatment in third molar removal

Submitted by Adriana Martinez (amartinez@unoeste.br) on 2017-09-05T12:53:44Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Luci.pdf: 503217 bytes, checksum: bcb750cc3835332fed12d8730ecb4254 (MD5) / Made available in DSpace on 2017-09-05T12:53:44Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Luci.pdf: 503217 bytes, checksum: bcb750cc3835332fed12d8730ecb4254 (MD5) Previous issue date: 2017-06-22 / This work aims to study the effects of omega 3 and / or vitamin B12 administration on sperm and histomorphometric parameters of the reproductive organs of Wistar rats and their correlation with the temperature of these regions. Sixteen rats were divided into four groups (n = 4 per group) who received daily injections for 30 days, being: Control Group - saline solution; Group Omega three- fish oil (Equaliv®) 1g / kg; Group B12 - vitamin B12 (Monovin B12 ®) 3μg; And Group Omega + B12 - fish oil 1g / kg and vitamin B12 3μg. Analyzed sperm morphology and the histomorphometries were evaluated by the Motic Images Plus® version 2.0 program. On a daily basis, thermographic images of areas of the body (FLIR E40®, Sweden) were analyzed and analyzed by the Flir Tools 2.1® program. Data were submitted to analysis of variance and Tukey test at 5%. Omega 3 reduced the seminiferous epithelium and B12 minimized this deleterious effect (P ≤ 0.05). The surface temperature of the scrotum was higher in group B12 (P ≤0.05). There was a high positive correlation between scrotum surface temperature and distal cytoplasmic droplet percentage (P = 0.678). / Este trabalho tem como objetivo estudar os efeitos da administração do ômega três e/ou vitamina B12 sobre parâmetros espermáticos e histomorfométricos dos órgãos reprodutivos de ratos Wistar e sua correlação com a temperatura dessas regiões. Utilizou-se 16 ratos, divididos em quatro grupos (n=4 por grupo) que receberam injeções diárias por 30 dias, sendo: Grupo Controle – solução salina; Grupo Ômega três– óleo de peixe (Equaliv®) 1g/kg; Grupo B12 – vitamina B12 (Monovin B12®) 3µg; e Grupo Ômega + B12 – óleo de peixe 1g/kg e vitamina B12 3µg. Analisados morfologia espermática e as histomorfometrias foram avaliados pelo programa Motic Images Plus® versão 2.0. Diariamente, foram obtidas imagens termográficas de áreas do corpo (FLIR E40®, Suécia) e analisadas pelo programa Flir Tools 2.1®. Os dados foram submetidos à análise de variância e teste de Tukey a 5%. O ômega 3 reduziu o epitélio seminífero e a B12 minimizou esse efeito deletério (P ≤ 0,05). A temperatura da superfície do escroto foi superior no grupo B12 (P ≤0,05). Houve alta correlação positiva entre temperatura da superfície do escroto e porcentagem de gota citoplasmática distal (P = 0,678).