The objective of this research was to evaluate of three pasture-based systems (PS) with bermuda grass (BG) + forage peanut + 75Kg de N/ha; BG + common vetch + 75Kg of N/ha and BG + 150Kg de N/ha. BG was planted in 2006. The forage peanut was planted in September in BG stand with three seedlings/m²; common vetch was over seeding in BG, in respective PS. Lactating Holstein cows receiving 1% of body weight/day complementary concentrate feed, were used for evaluation. The experiment was carried out from May 2010 to May 2011. In the pre grazing cycle the forage mass (PFM) botanical and structural composition, daily dry matter accumulation rate (DMA) and stocking rate (SR) were evaluated. The experimental design used was completely randomized were performed, with three treatment (PS), two replicates (paddocks) in completely, split-plot time. Eleven grazing cycles were performed during the experimental period (360 days). To determine crude protein (CP), neutral detergent fiber (NDF), dry matter in situ digestibility (DMISD), organic matter in situ digestibility (OMISD) and total digestible nutrients (TDN) samples were collected by the hand-plucking method. The mean values of PFM, DMA, SR, were 2.5; 2.6 and 2.5 t/ha; 58; 65 and 62 Kg of DM/ha/day; 6.0; 7.6 and 6.9 animal units/ha/day for respective PS. The mean values of CP, NDF, DMISD, OMISD and TDN were; 16.4; 22.2 and 15.3%; 68.3; 65.7 and 66.3%; 68.5; 70.3 and 66.4%; 64.3; 63.2 3and 65.1%; 57.7; 58.2 and 57.8% for respective PS. Residual effects of forage legume (common vetch) were observed on DMA and bermuda grass leaf blade percentage. Similar result for PFM and SR were found between BG + common vetch + 75 kg of N/ha and BG + 150 kg of N/ha. Towards the nutritive value the PS mixed to Coastcross-1 plus common vetch showed a better performance. / O objetivo desta pesquisa foi avaliar três sistemas forrageiros (SF), constituídos por capim bermuda (CB) + amendoim forrageiro + 75 kg de N/ha; CB + ervilhaca + 75 Kg de N/ha e CB + 150 Kg de N/ha. O CB foi estabelecido em 2006. O amendoim forrageiro foi plantado, em setembro, na área já estabelecida com CB, com três mudas/m². A ervilhaca foi sobressemeáda, em maio, na área do CB, nos respectivos SF. Para avaliação foram usadas vacas da raça Holandesa em lactação que receberam concentrado à razão de 1% do peso corporal/dia. O experimento foi conduzido entre maio de 2010 e maio de 2011. Avaliou-se a massa de forragem de pré pastejo (MFP), as composições botânica e estrutural, a taxa de acúmulo diário de matéria seca (TAC) e a taxa de lotação (TL). O delineamento experimental utilizado foi o inteiramente casualizado com três tratamentos (SF), duas repetições (piquetes) em parcelas subdivididas no tempo. Para determinar o teor de proteína bruta (PB), fibra em detergente neutro (FDN), digestibilidade in situ da matéria seca (DISMS), digestibilidade in situ da matéria orgânica (DISMO) e nutrientes digestíveis totais (NDT) foram coletadas amostras de forragem pelo método de simulação de pastejo. No período experimental (360 dias), foram conduzidos 11 ciclos de pastejo. As médias para MFP, TAC e TL foram de 2,5; 2,6 e 2,5 t/ha; 58; 65 e 62 Kg de MS/ha/dia; 6,0; 7,6 e 6,9 UA/ha/dia para os respectivos SF. Os valores médios de PB, FDN, DISMS, DISMO e NDT foram de 16,4; 22,2 e 15,3%; 68,3; 65,7 e 66,3%; 68,5; 70,3 e 66,4%, 64,3; 63,2, e 65,1%; 57,7; 58,2 e 57,8%, respectivamente. Foi observado efeito residual da leguminosa forrageira (ervilhaca) na TAC da pastagem e no porcentual de lâmina foliar do capim bermuda. Resultados similares quanto a MFP e TL foram observados entre os SF constituídos por CB + ervilhaca + 75 kg de N/ha e CB + 150 Kg de N/ha. Com relação ao valor nutritivo, verificou-se melhor resultado no SF constituído de Coastcross-1 e ervilhaca.

O teste alérgico intradérmico (TID) é uma importante ferramenta no diagnóstico dos alérgenos implicados nas reações de hipersensibilidade mediada por IgE. O objetivo deste estudo foi avaliar o TID em eqüinos não alérgicos com extratos alergênicos de Cynodon dactylon, Lolium multiflorum, Paspalum notatum, Culex sp. e Aedes aegipty, e três concentrações de histamina, em dois volumes diferentes, e baseado nos resultados, verificar a acurácia do TID em eqüinos alérgicos. Foram realizados TID em 17 eqüinos não alérgicos com três concentrações de cada extrato (1:2000v/w, 1:4000v/w e 1:8000v/w) e três concentrações de histamina (0,1mg/ml, 0,05mg/ml e 0,025mg/ml), em dois volumes (0,1 e 0,05ml). Baseado nos resultados obtidos, foram realizados TID em seis eqüinos alérgicos utilizando os extratos na concentração de 1:2000v/w, a histamina na concentração de 0,025mg/ml, em um volume de 0,05ml. Nos eqüinos não alérgicos, nenhuma das três concentrações dos extratos testados nos dois volumes mostrou-se irritativa. Concentrações maiores de histamina provocaram halos maiores e levemente mais túrgidos, e quanto maior o volume injetado, maiores as reações Os TID realizados em eqüinos alérgicos mostraram confiabilidade dos resultados por evidenciar hipersensibilidade individual e não provocar reações irritativas. Em conclusão, as diferentes concentrações dos estratos testados em eqüinos não alérgicos não provocaram reações falso positivas (irritantes). O volume de 0,05ml é mais recomendado para realização dos TID pois as aplicações com 0,1ml produziram reações maiores, prejudicando a sensibilidade do teste. A concentração de 0,025mg/ml da solução de histamina provocou a formação de halos menores, permitindo maior acurácia do cut off no TID. Os extratos alergênicos testados em equinos alérgicos não provoraram reações em todos os animais, o que poderia ser considerado uma reação irritativa. Os equinos alérgicos apresentaram reações positivas diferentes, de acordo com a hipersensibilidade individual, demonstrando sensibilização alérgica verdadeira. / The intradermal test (IDT) is an important tool in the diagnosis of allergens involved in IgE-mediated hypersensitivity reactions. The aim of this study was to evaluate the IDT in nonallergic horses with allergenic extracts of Cynodon dactylon, Lolium multiflorum, Paspalum notatum, Culex sp. and Aedes aegypti, and three histamine concentrations, in two different volumes, and based on the results, verify the accuracy of IDT in allergic horses. IDT was performed on 17 nonallergic horses with three concentrations of each extract (1:2000v/w, 1:4000v/h and 18000v/w) and three histamine concentrations (0,1mg/ml, 0,05mg/ml and 0,025mg/ml) in two volumes (0,1 and 0,05ml). Based on the results obtained, IDT was performed in six allergic horses using extracts at the concentration of 1:2000v/w, histamine at a concentration of 0.025mg/ml, in a volume of 0,05ml. In nonallergic horses, none of the three concentrations of the extracts tested in the two volumes were irritant. Larger concentrations of histamine provoked larger and slightly more turgid wheals, and the larger the volume injected, the greater the reactions The IDT performed in allergic horses showed reliability of the results because they demonstrated individual hypersensitivity and did not provoke irritative reactions. In conclusion, the different concentrations of extracts tested in nonallergic horses did not provoke false positive (irritant) reactions. The volume of 0,05ml is more recommended for execution of IDT because the applications with 0,1ml produced larger reactions, impairing the sensitivity of the test. The concentration of 0,025mg/ml of the histamine solution caused the formation of smaller wheals, allowing a better accuracy of the cut off in the IDT. The allergenic extracts tested in allergic horses did not provoke reactions in all the animals, what could be considered an irritative reaction. Allergic horses presented different positive reactions, according to individual hypersensitivity, demonstrating true allergic sensitization.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / In Brazil, most soils used in the livestock presents some fertility limitations. One of the alternatives to improve the soils quality is the introduction of perennial species, including grassy and associated legumes and correction of the soil that it will hold the cultures forage crop. There s a lack of Information and researches on soil quality improvement using perennial forages. Therefore, the objective of this work was to evaluate different systems of forage cultures constituted by Pennisetum americanum, Arachis pintoi and Stylosanthes guianensis associated with Cynodon dactylon and both isolated species, with and without mineral fertilizer, and evaluations of the attributes of a Ultisols. In both treatments, Cynodon dactylon received 100 kg of nitrogen during a year. The crops of forage samples were accomplished at every 28 days, making botanical separation and drying the same. After the weighting to calculate the dry matter per area (ha) the samples were crushed for analyses of fiber of neutral detergent (FDN), fiber of acid detergent (FDA), rude protein (PB), calcium (Ca), magnesium (Mg), phosphorus (P) and potassium (K). The samples collecting were taken during January, March, April and October of 2007. The best results of nutritional values were found in the legumes and in the consortium, being with inferior quality the grassy ones. The dry matter present a great variation among the cuts and the forage systems. After the winter the Stylosanthes portions were lost, due to the frosts that happened during this period. This confirms that it not has an adaptation on our conditions of winter. After a year of implantation of the experiment was collected soil to analysis: density of the soil (DS), microporosity (Mip) and macroporosity (Map) porosity and total porosity of the soil (PT) and size of aggregates. In the chemical part, were analyzed total nitrogen (NT), total organic carbon (COT) and phosphorus (P) and potassium (K). DS presented variation between the treatments and the appraised layers, since 1,40 to 1,62 Mg m-3. In Mip and PT didn't present significant differences between the treatments and layers, but Map presented significant differences. In the aggregate size there was significant differences, and the geometric medium diameter (DMG) it varied from 1,41 to 3,21 mm. In the soil chemical attributes both maintained similarities, in other words, larger concentration in the first layer (0 to 5 cm). But some treatments standing out in larger concentration in the first layer, however maintain similarities in the deep layers, unless the potassium (K), that has a distribution more constantly in the evaluated layers. / No Brasil, a maioria dos solos utilizados para pecuária apresenta algumas limitações de fertilidade. Uma das alternativas para melhorar as condições desses solos é a introdução de espécies perenes, incluindo gramíneas e leguminosas consorciadas e correção do solo que comportará as culturas forrageiras. Informações e pesquisas sobre melhorias de solos com uso de espécies forrageiras perenes são escassas. Nesse sentido, o objetivo deste trabalho foi avaliar diferentes sistemas de culturas forrageiras constituídas de milheto, amendoim forrageiro e estilosantes consorciados com tifton 85 e ambas as espécies isoladas, com e sem adubação mineral, e avaliações dos atributos de um Argissolo vermelho. Em ambas as parcelas adubadas e não adubadas, o tifton 85 recebeu 100 kg de nitrogênio ano-1. As colheitas de amostras de forragem eram realizadas a cada 28 dias, realizando-se separação botânica e secagem da mesma. Feita a pesagem para calcular matéria seca (MS) por área (ha) realizava-se a moagem das amostras para análises de fibra de detergente neutro (FDN), fibra de detergente ácido (FDA), proteína bruta (PB), cálcio (Ca), magnésio (Mg), fósforo (P) e potássio (K). Realizaram-se coletas nos meses de janeiro, março, abril e outubro de 2007. Os melhores resultados de valores de PB, FDA e FDN, foram encontrados nas leguminosas e no consórcio, ficando com qualidade inferior o tifton 85 + 100 kg ha-1 de N. Quanto à produção de MS, variou muito entre os cortes e os sistemas forrageiros. Após o inverno as parcelas de estilosantes foram perdidas, devido às geadas que ocorreram no período de inverno. Isso confirma que o mesmo não tolera frio e não se adapta a nossas condições de inverno. Após um ano de implantação do experimento realizou-se coletas para análise dos atributos do solo, sendo: densidade do solo (DS), micro (Mip) e macro (Map) porosidade e porosidade total do solo (PT) e tamanho de agregados. Na parte química, analisou-se nitrogênio total (NT), carbono orgânico total (COT) e teores de fósforo (P) e potássio (K). A DS apresentou variação entre os tratamentos e as camadas avaliadas, desde 1,40 a 1,62 Mg m-3. Na Mip e PT não apresentou diferenças significativas entre os tratamentos e camadas, mas o Map apresentou diferenças significativas. Nos tamanhos dos agregados houve diferenças significativas, sendo que o diâmetro médio geométrico (DMG) variou de 1,41 a 3,21 mm. Nos atributos químicos do solo, como NT, COT, P e K ambos mantiveram semelhanças, ou seja, maior concentração na primeira camada (0 a 5 cm). Mas alguns tratamentos se destacaram em maior concentração na primeira camada, mantendo semelhanças nas camadas mais profundas, exceto o K que teve uma distribuição mais uniforme nas camadas avaliadas.

Low temperature injury is among the greatest challenges facing golf courses with ultradwarf bermudagrass (UDB) (Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy) putting greens in Virginia. This research focused on the impact of turf covers, fungicide programming, core aeration, and trinexapac-ethyl (TE) on UDB cold tolerance, winter quality, and cold de-acclimation (CD). Our results indicate that the use of turf covers significantly increased UDB canopy and soil temperatures when air temperatures were below -3.9°C. Air gaps under covers and the use of double turf covers increased soil and canopy temperatures compared to single covers alone in some instances, but results were inconsistent. Late fall and early winter fungicide applications of chlorothalonil and azoxystrobin improved UDB quality throughout winter dormancy and spring green up. The addition of a pigmented phosphonate significantly improved winter and spring UDB quality. The addition of acibenzolar-S-methyl to fungicide programs did not improve winter UDB quality or spring green up. Summer core aeration programs were evaluated for their impact on spring green up, turfgrass quality, surface firmness, and moisture retention. Spring UDB green up was improved incrementally as surface disruption increased. Treatments with 20%, 15%, and 10% surface disruption produced higher color vs treatments with lower surface disruption. Surface firmness and volumetric water content of UDB were impacted by construction method but were not significantly impacted by core aeration programs. Field research revealed that 'fall only' and 'fall and winter' TE applications improved UDB quality but only 'fall and winter' delayed UDB premature CD in early spring when UDB can be susceptible to low temperature injury. Growth chamber studies evaluated the impact of TE on UDB cold tolerance to -9.4°C x time duration. Regression analysis predicted a 50% mortality exposure point for UDB under TE treatments of 9.84 hours at -9.4°C (r2=0.836) compared to 11.38 hours at -9.4°C (r2=0.671) for non-treated UDB during cold acclimation. Winter and spring scenarios resulted in delayed CD under TE but no differences in cold tolerance when exposed to -9.4°C. Together, these results increase our understanding of the impact of management practices on UDB winter quality, CD, and low temperature injury. / Doctor of Philosophy / Ultradwarf bermudagrass putting greens are commonly found on golf courses in warm climates. These grasses thrive in heat and humidity but are susceptible to injury or death when exposed to cold temperatures. This research is focused on evaluating management practices that may impact bermudagrass' susceptibility to injury from cold temperature exposure. The cultural practices evaluated include turf covers, fungicide programming, core aeration, and the use of plant growth regulators to manipulate the turfgrasses own self defense mechanisms. Our results show that the use of turf covers significantly increased putting green canopy and soil temperatures when air temperatures were below -3.9°C. Air gaps under covers and the use of double turf covers increased soil and canopy temperatures compared to single covers alone in some instances, but results were inconsistent. Late fall and early winter fungicide applications of commonly-used fungicides improved putting green quality throughout winter dormancy and spring green up. The addition of a green-pigmented phosphonate fungicide significantly improved winter and spring putting green quality. The addition of a plant defense activator, acibenzolar-S-methyl to fungicide programs did not improve winter quality or spring green up. Summer core aeration programs were evaluated for their impact on spring green up, turfgrass quality, surface firmness, and moisture retention. Spring green up was improved incrementally as surface disruption increased. Treatments with 20%, 15%, and 10% surface disruption produced higher color vs treatments with lower surface disruption. Surface firmness and soil moisture content of the putting greens were impacted by construction method but were not significantly impacted by core aeration programs. Field research revealed that 'fall only' and 'fall and winter' plant growth regulator applications improved ultradwarf bermudagrass quality but only 'fall and winter' delayed premature green-up in early spring when the turfgrass can be susceptible to low temperature injury. Growth chamber studies revealed that plants treated with the growth regulator, trinexapac-ethyl were more sensitive to low-temperature exposure than non-treated plants. Together, these results increase our understanding of the impact of management practices on UDB winter quality, CD, and low temperature injury.

Goosegrass [Eleusine indica (L.) Gaertn.] and smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. ex Muhl.] are problematic weeds in bermudagrass and creeping bentgrass turf. Increased incidences of herbicide resistant weed populations and severe use restrictions on formerly available herbicides have increased need for selective, postemergence control options for these weeds in creeping bentgrass and bermudagrass turf. This weed management exigency has led turf managers to utilize less effective, more expensive, and more injurious options to manage goosegrass and smooth crabgrass. Although potentially injurious, topramezone can control these weeds, especially goosegrass, at low doses. Low-dose topramezone may also improve bermudagrass and creeping bentgrass response. An initial investigation of three 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibiting herbicides in different turf types showed that Kentucky bluegrass, perennial ryegrass, and tall fescue were highly tolerant to topramezone, while creeping bentgrass and bermudagrass could tolerate topramezone doses that may control grassy weeds. Further investigation suggested that frequent, low-dose topramezone applications or metribuzin admixtures could enhance weed control and may conserve turfgrass quality. A novel mixture of topramezone at 3.7 g ae ha-1 and metribuzin at 210 g ai ha-1 controlled goosegrass effectively and reduced bermudagrass foliar bleaching associated with topramezone 10-fold compared to higher doses of topramezone alone in 19 field and 2 greenhouse trials. In an attempt to further enhance bermudagrass tolerance to topramezone, post-treatment irrigation was applied at various timings. When bermudagrass turf was irrigated with 0.25-cm water at 15 or 30 minutes after herbicide treatment, bermudagrass injury was reduced to acceptable levels when following low-dose topramezone plus metribuzin but not when following high-dose topramezone alone. Goosegrass control was reduced significantly by post-treatment irrigation in all cases, while irrigation reduced goosegrass control by low-dose topramezone plus metribuzin to below-commercially-acceptable levels. Novel, low-dose, frequent application programs containing topramezone or siduron were developed for season-long crabgrass or goosegrass control on creeping bentgrass greens. Greens-height creeping bentgrass quality was preserved following five biweekly treatments of siduron at rates between 3,400 to 13,500 g ai ha-1 and topramezone at 3.1 g ha-1. Siduron programs controlled smooth crabgrass and suppressed goosegrass while topramezone programs controlled goosegrass and suppressed smooth crabgrass. In laboratory and controlled-environment experiments, goosegrass absorbed three times more 14C than bermudagrass within 48 hours of 14C-topramezone treatment. Bermudagrass also metabolized topramezone twice as fast as goosegrass. Metribuzin admixture reduced absorption by 25% in both species. When herbicides were placed exclusively on soil, foliage, or soil plus foliage, topramezone controlled goosegrass only when applied to foliage and phytotoxicity of both bermudagrass and goosegrass was greater from topramezone than from metribuzin. Metribuzin was shown to reduce 21-d cumulative clipping weight and tiller production of both species while topramezone caused foliar discoloration to newly emerging leaves and shoots with only marginal clipping weight reduction. These data suggest that selectivity between bermudagrass and goosegrass is largely due to differential absorption and metabolism that reduces bermudagrass exposure to topramezone. Post-treatment irrigation likely reduces topramezone rate load with a concomitant effect on plant phytotoxicity of both species. Metribuzin admixture decreases white discoloration of bermudagrass by decreased topramezone absorption rate and eliminating new foliar growth that is more susceptible to discoloration by topramezone. / Doctor of Philosophy / Goosegrass and smooth crabgrass are problematic weeds in bermudagrass and creeping bentgrass turf. Increased incidences of herbicide resistant weed populations and severe use restrictions on formerly available herbicides have increased need for selective, postemergence control options for these weeds in creeping bentgrass and bermudagrass turf. Although potentially injurious, topramezone (Pylex™) can control these weeds, especially goosegrass, at low doses. Low-dose Pylex™ may also improve bermudagrass and creeping bentgrass response. An initial investigation evaluating tembotrione (Laudis®), Pylex™, and mesotrione (Tenacity®) in different turfgrass species showed that Kentucky bluegrass, perennial ryegrass, and tall fescue were highly tolerant to Pylex™ at rates ranging from 0.75 to 2.25 fl. oz./A, while creeping bentgrass and bermudagrass were low to moderately tolerant to Pylex™. Further investigation suggested that frequent, low-dose (less than 0.25 fl. oz./A) Pylex™ applications or metribuzin (Sencor®) admixtures could enhance weed control and may conserve turfgrass quality. A novel mixture of Pylex™ at 0.15 fl. oz./A and Sencor® at 4 oz. wt./A controlled goosegrass effectively and reduced bermudagrass injury to near acceptable levels and significantly less than Pylex™ applied alone at 0.25 fl. oz/A. In an attempt to further enhance bermudagrass tolerance to Pylex™, post-treatment irrigation was applied at different timings. When bermudagrass turf was irrigated at 15 or 30 minutes after herbicide treatment, bermudagrass injury was reduced to acceptable levels when following Pylex™ at 0.25 fl. oz./A plus Sencor® at 4 oz but not when following Pylex™ applied alone at 0.5 fl. oz./A. Goosegrass control was reduced significantly by post-treatment irrigation in all cases, while irrigation reduced goosegrass control by low-dose Pylex™ plus Sencor® to below-commercially-acceptable levels. Novel, low-dose, frequent application programs containing Pylex™ or siduron (Tupersan®) were developed for season-long crabgrass or goosegrass control in creeping bentgrass greens. Greens-height creeping bentgrass quality was preserved following five biweekly treatments of Tupersan® at rates between 6 and 24 lb./A and Pylex™ at 0.125 fl. oz./A. Tupersan® programs controlled smooth crabgrass and suppressed goosegrass while Pylex™ programs controlled goosegrass and suppressed smooth crabgrass. The data from these studies indicate that utilizing low-dose Pylex™ in combination with Sencor® can impart acceptable bermudagrass safety while also controlling goosegrass effectively. For creeping bentgrass greens, the low-dose, frequent application of Tupersan® is the safest legal option for golf course superintendents to control smooth crabgrass effectively, while having some ability to suppress goosegrass.

A trial was conducted to evaluate forage production potential, forage intake and milk production in two perennial tropical grasses under continuous grazing of lactating cows, in the growth seasons of 2003/04 and 2004/05, in Palmeira das Missões/RS. Twelve tester animaIs were distributed in two paddocks per treatment, using "put-and-take" technique to adjust stocking rates. Evaluated treatments were Dwarf Elephantgrass (Pennisetum purpureum cv. Mott) and Tifton 85 (Cynodon dactylon x C. nlemfuensis) pastures. Under the proposed management criteria (4 % of leaf lamina allowance), Tifton 85 demonstrates to be less sensible to forage production and stocking rate reduction under adverse climate conditions, with relative stability of forage mass available. Its superiority in individual animal performance, in more favourable conditions (2003/04 growth season), when compared to Dwarf Elephantgrass, should be related to the allowance used. Maintaining low levels of leaf lamina on offer could be limiting to cows' individual performance, rebounding negatively over persistency of the pastures. The evaluated species presented different structures and forage densities in the most accessible strata to grazing by the animaIs. However, those differences didn't affect the chemical composition of these layers nor of its respective of its respective "hand-plucking" samples, causing similar forage intake. Higher individual animal production indices were found, indicating a little known potential for perennial tropical grasses in milk from pasture systems. / Para avaliar o potencial forrageiro, o consumo de forragem e a produção de leite em duas gramíneas perenes tropicais sob pastejo contínuo de vacas em lactação, foi conduzido um experimento nos anos agrícolas de 2003/04 e 2004/05, em Palmeira das Missões/RS. Foram utilizados 12 animais testes, distribuídos em dois piquetes por tratamento, adotando-se a técnica dos animais reguladores para ajustes da carga animal. Os tratamentos avaliados foram pastagens de Capim Elefante Anão (Pennisetum purpureum cv. Mott) e Tifton 85 (Cynodon dactylon x C. nlemfuensis). Sob o critério de manejo proposto (oferta de 4 % de lâminas foliares), o Tifton 85 demonstra ser menos sensível à redução da produção forrageira e capacidade de carga sob condições adversas de clima, com relativa manutenção da massa de forragem disponível. Sua superioridade em produção individual por animal, em condições mais favoráveis (ano agrícola 2003/04), quando comparado ao Capim Elefante Anão, deve estar relacionada à oferta utilizada. A manutenção de níveis reduzidos de oferta de lâminas foliares pode ter sido limitante ao desempenho individual das vacas, repercutindo também de forma negativa na persistência produtiva das pastagens. As espécies estudadas apresentaram distintas estruturas e densidades de forragem nos estratos mais acessíveis ao pastejo pelos animais. Contudo, tais diferenças estruturais não afetaram a composição química destas camadas ou das respectivas simulações de pastejo, acarretando em consumo de forragem similar. Foram registrados elevados índices de produção animal individual, indicando um potencial pouco conhecido das gramíneas tropicais em sistemas de produção de leite a pasto.

Field studies were conducted during 2006 and 2007 to evaluate control of saw greenbriar and southern dewberry by various pasture herbicides and to assess forage tolerance of Tifton 85 bermudagrass to these herbicides. Herbicides evaluated in each study included triclopyr, picloram, 2,4-D, fluroxypyr, dicamba, aminopyralid, metsulfuron methyl and various combinations of the above. Visual ratings were taken on each herbicide efficacy experiment. Visual evaluations of phytotoxicity, measurements of dry matter yield, and forage quality were quantified for each of the bermudagrass tolerance trials. Saw greenbriar was best controlled at approximately one year after treatment by triclopyr at 10.9% ae v/v with diesel as the carrier (88-98%), although the lower rate of triclopyr + diesel at 0.87% ae v/v + 5% v/v and triclopyr alone at 0.87% ae v/v provided 49 to 86% control. Triclopyr + fluroxypyr at 0.25% ai v/v + 0.086% ai v/v gave best control of southern dewberry in both years when applied as an individual plant treatment (IPT) six weeks after shredding. In general, shredding 45 days prior to herbicide application gave an advantage to southern dewberry control versus not shredding. In 2006, triclopyr + fluroxypyr (IPT) was the only treatment to decrease Tifton 85 dry matter yield at the first harvest, with no effect observed at the second harvest. In 2007, both broadcast treatments containing triclopyr + fluroxypyr and the IPT treatment of triclopyr decreased dry matter yield at the first harvest, with triclopyr (IPT) being the only treatment to lower dry matter yield at the second harvest. Field studies were also conducted in 2006 and 2007 to assess sharppod morningglory control in Roundup Ready Flex® and LibertyLink® cotton systems. Herbicides evaluated included glyphosate, glufosinate, prometryn, fluometuron, and diuron. Visual ratings of percent weed control and sharppod morningglory plant counts were taken to assess control. Prometryn at 1.8 kg ai ha⁻¹ and fluometuron at 1.8 kg ai ha⁻¹ provided significant preemergence control (33-81%) of seedling sharppod morningglory. All rates of glyphosate (1.06 and 1.54 kg ai ha⁻¹) and glufosinate (0.45 and 0.6 kg ai ha⁻¹) controlled sharppod morningglory from 55 to 100% at both application timings. The addition of diuron at 1.12 kg ai ha⁻¹ to glyphosate and glufosinate at the late season application enhanced sharppod morningglory control by 3 to 16%. Additionally, in both years, no reduction in cotton yield was observed in the morningglory infested treatment when compared to the weed free treatment.

As average global temperatures rise, cool-season C3 turfgrasses, such as the most commonly grown Kentucky bluegrass (Poa pratensis L.; KBG), struggle to tolerate extreme summer heat and increase their water consumption. Hybrid Bermudagrass (Cynodon dactylon [L.] Pers. × Cynodon transvaalensis Burtt Davy; HBG) is a warm-season C4 grass that may be increasingly suited for northern ecosystems traditionally classified as transition or cool-season climate zones. Glasshouse and field studies were conducted to compare HBG and KBG water use. The objective of the glasshouse study was to evaluate plant health and growth for two HBG cultivars (‘DT-1’ and ‘NorthBridge’) compared to a blend of KBG cultivars in all combinations of deficit, moderate, and high irrigation at optimum or short mowing height. The study was conducted in a glasshouse at Provo, UT, USA from 2020-2021. Grass was grown in pots arranged in a randomized complete block, full factorial design, with four replications of each treatment. The moderate KBG was also significantly different from both high and deficit for verdure and for the last half of NDVI. The objective of the field study was to evaluate two HBG cultivars (‘Tahoma 31’ and ‘Latitude 36’) compared to a blend of KBG cultivars for water loss and canopy health, temperature, and growth when subjected to deficit, moderate, and high irrigation. The study was arranged in a randomized complete block, full factorial design with three replications per treatment, and was conducted at Provo, UT, USA throughout the summer of 2021. In both the glasshouse and field trials, the deficit irrigated KBG consistently scored lower for NDVI and visual turf quality than all other treatments, including moderate and high KBG. This same trend was seen in the field study for percent cover. Although not observed in the glasshouse trial, it was observed in the field trial that the different irrigation levels of HBG resulted in no significant differences for any measurements but the HBG regularly scored better than KBG. The canopy temperatures of deficit irrigated KBG were also higher than all other treatments on most dates. The shoot mass, thatch mass, and total biomass of KBG were significantly less than either HBG cultivar. In the glasshouse trial it was observed that all deficit grasses were significantly lower than the other irrigation treatments and HBG had significantly deeper roots than KBG, although these results were not seen in the field trial. The data suggest that irrigation needs will be less for HBG than KBG and that HBG could provide a water-saving turfgrass alternative to KBG in semi-arid, cool-season regions with increasing water scarcity.

Bermudagrass (Cynodon spp.) breeding and cultivar development is hampered by limited information regarding its genetic and phenotypic diversity. A germplasm collection of 206 bermudagrass accessions from 29 countries was genotyped with high-throughput genotyping-by-sequencing technique. Genomic diversity in this diverse germplasm panel was assessed with multifaceted approaches including population structure, phylogenetic analysis, principal component analysis, and genetic diversity parameters. This study revealed substantial genetic variation in the Cynodon accessions, demonstrating the potential of this germplasm panel for further genetic studies and cultivar development in breeding programs. Another critical issue in turfgrass breeding is the lack of information regarding the genetic architecture of traits. Four agronomic traits leaf length, leaf width, internode distance and stem diameter were evaluated in a germplasm panel of common bermudagrass accessions. Then genome-wide association study was performed to dissect the genetic basis of the traits.

MSc (Botany) / Department of Botany / The use of disturbance in the management of Savanna is a common rangeland practice in South Africa. A two-season trial was established in June 2016 and December 2016 to evaluate the effects of disturbance of different intensities on wetland belts in the Nylsvley Nature Reserve. The disturbances were in three different belts of different grass types at different intensities. The experimental treatments consisted of two different intensities, namely; high (H) and low (L). The Cyperus amabilis belt was regarded as site 1, the Sporobolus africanus as site 2, and the Themeda triandra as site 3. Seventeen kites were contracted in each belt, two times 1 m × 1 m at high and low intensity, followed by two times 2 m × 2 m one at high intensity. The results showed that disturbance affects the topsoil by removing the soil which is rich in nutrients producing pioneer species for the first few months and then original species were seen inhibiting again. / NRF